Rroxscaffold_1G00043870

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013269
Physical Location & Seq
Forward (+)
62551734 .. 62554549
2816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00043870.1

Sequence Viewer

Length: 1119 bp
ATGGAGAGGCCCCCAAGTAACTGCAGCCCAAGATCCATAGGGAGCAGTGAGTTCCTCAACCCAATAACCCATGAAGTCATTTTGAGCAACTCAAGGTGTGAGCTCAAAATCACTAGCTTTGATATTGGCAATGGCCACAGCAACAATGGAGGTGGGAATAGCAGTAATGGTGGTTCAACATTTGCAGATACCCTAAAGCCAGGCACACCTGGGCCTGGAGGCCACCCCACAATGCACTATTTCAACCCTTCTTTTAGGACCTCAGCCACTACTCCTCCAAGGAAGAGCCCTTTTCGGGTTTCTGCAGATACAGAAGGCTCTGGAAATTTTAAGGGTTCTTCAGGTTTGAATTCAGCTAGTGGGATTGGACTTCGCCAAAGCAATAGGGTCTCTTTTGATGGTTCTACCTCAATTACTTGTCACAAATGTGGTGAGCAGTTTACAAAATGGGAAGCTGCTGAAGCACACCATCTCTCTAAACATGCTGTTACTGAACTTATGGAAGGTGACTCGTCTAGAAAAATTGTTGAGATAATATGCCGGACAAGCTGGTTGAAATCCGAGAACAATTGTGGAAGGATTGAGAGAGTGTTGAAAGTGCACAACATGCAAAGAACTCTTGCTCGATTCGAAGAATATCGAGAGATGGTGAAGATCAAAGCCAGCAAACTCCCTAAGAAACACCCAAGGTGCATTGCTGATGGGAATGAACTTTTGAGGTTTTATGGGACTACAGTGGCCTGCTCTCTCGGCCTAAATAGTTCCTCAAGTCTTTGTGTATCAGAAAAATGCTGTGTTTGTAGGATTATAAGAAATGGGTTCTGTGCAAAGAAGGAGCTCAAGGAAGGTGTAGGTGTTTTCACAACTTCCACAAGCGCAAGAGCCTATGAATGCATTGAAATATTGGAAGAGGATCCATGTATTAGGAAAGCTTTGATAGTCTGCAGAGTAATTGCTGGGAGGGTTCATAAGCCTTTGGAGAACATAAAAGAAATTGCTGGACAAACTGGTTTTGATTCTTTGGCTGGAAAAATGGGTCTTTACTCAAATATTGAAGAGCTTTATCTGCTCAATCCTAGAGCTCTCCTTCCTTGCTTTGTGGTAATCTGCAAACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

372

Amino Acids

40.61

Weight (kDa)

8.95

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54630
fragaria_vesca FvH4_3g21630 FvH4_3g21630
malus_domestica MD03G1214000.v1.1 MD11G1229000.v1.1
prunus_persica Prupe.4G200600_v2.0.a1 Prupe.4G200600_v2.0.a1
pyrus_communis pycom11g20330
rosa_chinensis RchiOBHm_Chr5g0037081
rosa_laevigata RLG00000033750
rosa_multiflora Rmu_ssc0000260.1_g000002
rosa_roxburghii Rroxscaffold_1G00043870
rosa_rugosa Rorug05G0160300
rosa_samantha Rh5AG252400 Rh5BG254200 Rh5CG286800 Rh5DG262700
rosa_wichuraiana Rw5G023290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 809
AclWI GGATC 3 cut(s) 27, 908, 921
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 2 cut(s) 325, 349
AcuI CTGAAG 2 cut(s) 324, 480
AfiI CCNNNNNNNGG 2 cut(s) 215, 295
AgsI TTSAA 7 cut(s) 177, 244, 349, 556, 595, 899, 1055
AjnI CCWGG 3 cut(s) 199, 208, 214
AleI CACNNNNGTG 1 cut(s) 426
AluBI AGCT 9 cut(s) 103, 117, 356, 455, 549, 838, 932, 1060, 1082
AluI AGCT 9 cut(s) 103, 117, 356, 455, 549, 838, 932, 1060, 1082
Alw21I GWGCWC 4 cut(s) 105, 603, 840, 1084
Alw26I GTCTC 1 cut(s) 394
Alw44I GTGCAC 1 cut(s) 599
AlwI GGATC 3 cut(s) 27, 908, 921
AoxI GGCC 6 cut(s) 8, 133, 212, 220, 738, 751
ApaLI GTGCAC 1 cut(s) 599
ApeKI GCWGC 2 cut(s) 24, 455
ApoI RAATTY 2 cut(s) 325, 349
AspLEI GCGC 1 cut(s) 878
AspS9I GGNCC 3 cut(s) 9, 212, 258
AsuHPI GGTGA 3 cut(s) 443, 518, 661
AsuII TTCGAA 1 cut(s) 630
AvaII GGWCC 1 cut(s) 258
BaeGI GKGCMC 1 cut(s) 603
BalI TGGCCA 1 cut(s) 135
BamHI GGATCC 1 cut(s) 913
BanII GRGCYC 4 cut(s) 105, 290, 840, 1084
Bbv12I GWGCWC 4 cut(s) 105, 603, 840, 1084
BbvCI CCTCAGC 1 cut(s) 262
BbvI GCAGC 2 cut(s) 36, 442
BccI CCATC 4 cut(s) 392, 477, 640, 695
BciT130I CCWGG 3 cut(s) 201, 210, 216
BcoDI GTCTC 1 cut(s) 394
BfaI CTAG 4 cut(s) 114, 357, 516, 1077
BfmI CTRYAG 4 cut(s) 22, 303, 732, 943
BisI GCNGC 2 cut(s) 25, 456
BlsI GCNGC 2 cut(s) 26, 457
Bme1390I CCNGG 3 cut(s) 201, 210, 216
Bme18I GGWCC 1 cut(s) 258
BmgT120I GGNCC 3 cut(s) 9, 212, 258
BmiI GGNNCC 2 cut(s) 11, 915
BmrFI CCNGG 3 cut(s) 201, 210, 216
BpmI CTGGAG 1 cut(s) 237
Bpu10I CCTNAGC 1 cut(s) 262
Bpu14I TTCGAA 1 cut(s) 630
BpuEI CTTGAG 3 cut(s) 76, 751, 824
BsaI GGTCTC 1 cut(s) 394
BsaJI CCNNGG 3 cut(s) 209, 278, 686
BsaXI ACNNNNNCTCC 2 cut(s) 259, 289
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 295
Bse1I ACTGG 1 cut(s) 1012
Bse3DI GCAATG 2 cut(s) 136, 693
BseBI CCWGG 3 cut(s) 201, 210, 216
BseDI CCNNGG 3 cut(s) 209, 278, 686
BseLI CCNNNNNNNGG 2 cut(s) 215, 295
BseMI GCAATG 2 cut(s) 136, 693
BseMII CTCAG 1 cut(s) 276
BseNI ACTGG 1 cut(s) 1012
BseRI GAGGAG 1 cut(s) 264
BseSI GKGCMC 1 cut(s) 603
BseXI GCAGC 2 cut(s) 36, 442
BseYI CCCAGC 1 cut(s) 956
BshFI GGCC 6 cut(s) 10, 135, 214, 222, 740, 753
BsiHKAI GWGCWC 4 cut(s) 105, 603, 840, 1084
BsiSI CCGG 1 cut(s) 541
BslFI GGGAC 1 cut(s) 742
BslI CCNNNNNNNGG 2 cut(s) 215, 295
BsmAI GTCTC 1 cut(s) 394
BsmFI GGGAC 1 cut(s) 742
BsmI GAATGC 1 cut(s) 896
BsnI GGCC 6 cut(s) 10, 135, 214, 222, 740, 753
Bso31I GGTCTC 1 cut(s) 394
Bsp119I TTCGAA 1 cut(s) 630
Bsp1286I GDGCHC 5 cut(s) 105, 290, 603, 840, 1084
Bsp143I GATC 3 cut(s) 32, 654, 913
BspANI GGCC 6 cut(s) 10, 135, 214, 222, 740, 753
BspCNI CTCAG 1 cut(s) 275
BspLI GGNNCC 2 cut(s) 11, 915
BspMAI CTGCAG 3 cut(s) 26, 307, 947
BspPI GGATC 3 cut(s) 27, 908, 921
BspQI GCTCTTC 2 cut(s) 278, 1050
BspT104I TTCGAA 1 cut(s) 630
BspTNI GGTCTC 1 cut(s) 394
BsrDI GCAATG 2 cut(s) 136, 693
BsrI ACTGG 1 cut(s) 1012
BssECI CCNNGG 3 cut(s) 209, 278, 686
BssMI GATC 3 cut(s) 32, 654, 913
BssT1I CCWWGG 2 cut(s) 278, 686
Bst2UI CCWGG 3 cut(s) 201, 210, 216
Bst4CI ACNGT 1 cut(s) 736
Bst6I CTCTTC 3 cut(s) 278, 903, 1050
BstAPI GCANNNNNTGC 1 cut(s) 607
BstBI TTCGAA 1 cut(s) 630
BstC8I GCNNGC 2 cut(s) 664, 742
BstDEI CTNAG 2 cut(s) 262, 675
BstHHI GCGC 1 cut(s) 878
BstKTI GATC 3 cut(s) 35, 657, 916
BstMAI GTCTC 1 cut(s) 394
BstMBI GATC 3 cut(s) 32, 654, 913
BstMWI GCNNNNNNNGC 5 cut(s) 461, 546, 607, 750, 1066
BstNI CCWGG 3 cut(s) 201, 210, 216
BstNSI RCATGY 2 cut(s) 485, 610
BstSCI CCNGG 3 cut(s) 199, 208, 214
BstSFI CTRYAG 4 cut(s) 22, 303, 732, 943
BstSLI GKGCMC 1 cut(s) 603
BstV1I GCAGC 2 cut(s) 36, 442
BstX2I RGATCY 2 cut(s) 32, 913
BstYI RGATCY 2 cut(s) 32, 913
BsuRI GGCC 6 cut(s) 10, 135, 214, 222, 740, 753
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 2 cut(s) 52, 741
Cac8I GCNNGC 2 cut(s) 664, 742
CfoI GCGC 1 cut(s) 878
Cfr13I GGNCC 3 cut(s) 9, 212, 258
CspCI CAANNNNNGTGG 2 cut(s) 133, 168
CviAII CATG 4 cut(s) 71, 482, 607, 918
DdeI CTNAG 2 cut(s) 262, 675
DpnI GATC 3 cut(s) 34, 656, 915
DpnII GATC 3 cut(s) 32, 654, 913
EaeI YGGCCR 1 cut(s) 133
Eam1104I CTCTTC 3 cut(s) 278, 903, 1050
EarI CTCTTC 3 cut(s) 278, 903, 1050
Ecl136II GAGCTC 3 cut(s) 103, 838, 1082
Eco130I CCWWGG 2 cut(s) 278, 686
Eco24I GRGCYC 4 cut(s) 105, 290, 840, 1084
Eco31I GGTCTC 1 cut(s) 394
Eco47I GGWCC 1 cut(s) 258
Eco53kI GAGCTC 3 cut(s) 103, 838, 1082
Eco57I CTGAAG 2 cut(s) 324, 480
EcoICRI GAGCTC 3 cut(s) 103, 838, 1082
EcoO109I RGGNCCY 2 cut(s) 9, 258
EcoRI GAATTC 1 cut(s) 349
EcoRII CCWGG 3 cut(s) 199, 208, 214
EcoT14I CCWWGG 2 cut(s) 278, 686
EcoT22I ATGCAT 1 cut(s) 896
EcoT38I GRGCYC 4 cut(s) 105, 290, 840, 1084
ErhI CCWWGG 2 cut(s) 278, 686
FaeI CATG 4 cut(s) 74, 485, 610, 921
FaqI GGGAC 1 cut(s) 742
FatI CATG 4 cut(s) 70, 481, 606, 917
Fnu4HI GCNGC 2 cut(s) 25, 456
FriOI GRGCYC 4 cut(s) 105, 290, 840, 1084
Fsp4HI GCNGC 2 cut(s) 25, 456
FspBI CTAG 4 cut(s) 114, 357, 516, 1077
GlaI GCGC 1 cut(s) 877
GluI GCNGC 2 cut(s) 25, 456
GsaI CCCAGC 1 cut(s) 960
GsuI CTGGAG 1 cut(s) 237
HaeIII GGCC 6 cut(s) 10, 135, 214, 222, 740, 753
HapII CCGG 1 cut(s) 541
HhaI GCGC 1 cut(s) 878
Hin1II CATG 4 cut(s) 74, 485, 610, 921
Hin6I GCGC 1 cut(s) 876
HinP1I GCGC 1 cut(s) 876
HindIII AAGCTT 1 cut(s) 930
HinfI GANTC 3 cut(s) 509, 627, 1016
HpaII CCGG 1 cut(s) 541
HphI GGTGA 3 cut(s) 443, 518, 661
Hpy166II GTNNAC 2 cut(s) 441, 601
Hpy188I TCNGA 2 cut(s) 562, 784
Hpy188III TCNNGA 3 cut(s) 321, 516, 641
Hpy8I GTNNAC 2 cut(s) 441, 601
HpyAV CCTTC 7 cut(s) 258, 308, 497, 570, 826, 839, 1097
HpyCH4III ACNGT 1 cut(s) 736
HpyF10VI GCNNNNNNNGC 5 cut(s) 461, 546, 607, 750, 1066
HpyF3I CTNAG 2 cut(s) 262, 675
Hsp92II CATG 4 cut(s) 74, 485, 610, 921
HspAI GCGC 1 cut(s) 876
Kzo9I GATC 3 cut(s) 32, 654, 913
LguI GCTCTTC 2 cut(s) 278, 1050
LmnI GCTCC 2 cut(s) 42, 835
Lsp1109I GCAGC 2 cut(s) 36, 442
MaeI CTAG 4 cut(s) 114, 357, 516, 1077
MaeIII GTNAC 4 cut(s) 17, 419, 487, 506
MalI GATC 3 cut(s) 34, 656, 915
MboI GATC 3 cut(s) 32, 654, 913
MboII GAAGA 6 cut(s) 295, 330, 644, 664, 920, 1067
MfeI CAATTG 1 cut(s) 568
MflI RGATCY 2 cut(s) 32, 913
MhlI GDGCHC 5 cut(s) 105, 290, 603, 840, 1084
MlsI TGGCCA 1 cut(s) 135
MluCI AATT 7 cut(s) 325, 349, 411, 522, 568, 951, 993
MluNI TGGCCA 1 cut(s) 135
MlyI GAGTC 1 cut(s) 503
Mox20I TGGCCA 1 cut(s) 135
Mph1103I ATGCAT 1 cut(s) 896
MscI TGGCCA 1 cut(s) 135
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 426
Msp20I TGGCCA 1 cut(s) 135
MspI CCGG 1 cut(s) 541
MspR9I CCNGG 3 cut(s) 201, 210, 216
MunI CAATTG 1 cut(s) 568
Mva1269I GAATGC 1 cut(s) 896
MvaI CCWGG 3 cut(s) 201, 210, 216
MwoI GCNNNNNNNGC 5 cut(s) 461, 546, 607, 750, 1066
NdeII GATC 3 cut(s) 32, 654, 913
NlaIII CATG 4 cut(s) 74, 485, 610, 921
NlaIV GGNNCC 2 cut(s) 11, 915
NmeAIII GCCGAG 1 cut(s) 729
NmuCI GTSAC 2 cut(s) 419, 506
NsiI ATGCAT 1 cut(s) 896
NspI RCATGY 2 cut(s) 485, 610
NspV TTCGAA 1 cut(s) 630
OliI CACNNNNGTG 1 cut(s) 426
PciSI GCTCTTC 2 cut(s) 278, 1050
PctI GAATGC 1 cut(s) 896
PfeI GAWTC 2 cut(s) 627, 1016
PkrI GCNGC 2 cut(s) 26, 457
PleI GAGTC 1 cut(s) 503
PpsI GAGTC 1 cut(s) 503
PpuMI RGGWCCY 1 cut(s) 258
PsiI TTATAA 1 cut(s) 809
Psp124BI GAGCTC 3 cut(s) 105, 840, 1084
Psp5II RGGWCCY 1 cut(s) 258
Psp6I CCWGG 3 cut(s) 199, 208, 214
PspFI CCCAGC 1 cut(s) 956
PspGI CCWGG 3 cut(s) 199, 208, 214
PspN4I GGNNCC 2 cut(s) 11, 915
PspPI GGNCC 3 cut(s) 9, 212, 258
PspPPI RGGWCCY 1 cut(s) 258
PsrI GAACNNNNNNTAC 2 cut(s) 157, 189
PstI CTGCAG 3 cut(s) 26, 307, 947
PsuI RGATCY 2 cut(s) 32, 913
RseI CAYNNNNRTG 1 cut(s) 426
SacI GAGCTC 3 cut(s) 105, 840, 1084
SapI GCTCTTC 2 cut(s) 278, 1050
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 2 cut(s) 25, 456
Sau3AI GATC 3 cut(s) 32, 654, 913
Sau96I GGNCC 3 cut(s) 9, 212, 258
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 3 cut(s) 201, 210, 216
SduI GDGCHC 5 cut(s) 105, 290, 603, 840, 1084
SfcI CTRYAG 4 cut(s) 22, 303, 732, 943
SfuI TTCGAA 1 cut(s) 630
SinI GGWCC 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 426
SmlI CTYRAG 3 cut(s) 91, 766, 839
SmoI CTYRAG 3 cut(s) 91, 766, 839
Sse9I AATT 7 cut(s) 325, 349, 411, 522, 568, 951, 993
SspI AATATT 2 cut(s) 903, 1051
SspMI CTAG 4 cut(s) 114, 357, 516, 1077
SstI GAGCTC 3 cut(s) 105, 840, 1084
StyD4I CCNGG 3 cut(s) 199, 208, 214
StyI CCWWGG 2 cut(s) 278, 686
TaaI ACNGT 1 cut(s) 736
TaqI TCGA 3 cut(s) 625, 630, 640
TasI AATT 7 cut(s) 325, 349, 411, 522, 568, 951, 993
TfiI GAWTC 2 cut(s) 627, 1016
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 2 cut(s) 52, 741
TseFI GTSAC 2 cut(s) 419, 506
TseI GCWGC 2 cut(s) 24, 455
Tsp45I GTSAC 2 cut(s) 419, 506
TspDTI ATGAA 4 cut(s) 87, 723, 903, 956
TspRI CASTG 2 cut(s) 52, 741
VneI GTGCAC 1 cut(s) 599
VpaK11BI GGWCC 1 cut(s) 258
XapI RAATTY 2 cut(s) 325, 349
XbaI TCTAGA 1 cut(s) 515
XceI RCATGY 2 cut(s) 485, 610
XcmI CCANNNNNNNNNTGG 1 cut(s) 143
XspI CTAG 4 cut(s) 114, 357, 516, 1077
Zsp2I ATGCAT 1 cut(s) 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.