Prupe.4G200600_v2.0.a1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0013269
Physical Location & Seq
Reverse (-)
12470219 .. 12473243
3025 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G200600.2

Sequence Viewer

Length: 1296 bp
ATGCCAACAGTGTGGTTCTCTCTGAAGAAATCTCTACACTGCAAATCAGAGCGATCAGAAGTTCATGATCCAAAGTCAAGGAGGCAGTTGAGCACAATCTTGACAAGAAAGGGAGGGAGGTCAGGCTGTTCAAGGTCTATAGCAAACCTCAAGGATGTGATTCATGGAAGCAAGAGACATTTGGAAAGGCCACCAAGTAATTGCAGCCCAAGATCTATAGGGAGCAGTGAGTTTCTGAACCCAATAACCCATGAAGTAATTTTGAGCAACTCGAGGTGTGAGCTCAAAATCACAAGCCTTGGTGAAGGAATTGGGTCTGGAAACATCAACAATGGTGGTGGTGGTTCAACATTTGTGGATACTCTAAGGCCAGGCACACCTGGCCCTGGTGGTCAATCAACAGTGCACTATTTTAATCCATCTTTTAGGACCTCATCAACTCCTCCAAGGAAAAGTCCTTTTCTTGCATCTGCAGATAATACAGAAGGGTCAGGGAAAGGTTCAGCTTTTCCTGGTAGTGGTGGAATTCGTCAGAGCAATAGGGTCTCCTTTGACACAGTCGCAGAGAACTCTCATGGTTCTTCTGCAATAACTTGTCACAAATGTGGTGAGCATTTTACCAAATGGGAAGCTGCTGAAGCACACCATCTCTCTAAACATGCTGTTACTGAACTTATGGAAGGTGACTCATCTAGAAAAATTGTCGAAATAATATGCCGGACAAGCTGGTTGAAATCCGAGAACAATTGTGGCCGGATTGAGAGAGTGTTGAAAGTCCACAACATGCAAAGAACTCTTGCTCGATTCGAAGAATATCGAGAGATGGTGAAAATGAAAGCCAGCAAACTCCCTAAGAAACACCCTAGGTGCATTGCTGATGGGAATGAGCTTTTGAGGTTTTATGGCACCACTGTGGCCTGCTCCCTTGGTCTAAATAGTTCCTCAAGTCTCTGTGTATCAGAAAAATGCTGTGTTTGTAGGATCATAAGAAATGGGTTCTCTGCAAAGAAGGAGCTCAAGGAAGGGGTAGGTGTTTTTACAACTTCAACAAGTGGAAGAGCTTTTGAGTCCATTCAAATATTAGAAGAAGATCCCTCCATAAGGAAAGTTTTGATAGTCTGCAGAGTAATTGCTGGGAGGGTTCATAAGCCTTTAGAGAATATACAAGAAATTGCCGGCCAAACTGGGTTTGATTCTTTGGCTGGAAAAATGGGTCTTTACTCAAATATTGAAGAGCTTTATCTGCTCAATCCTCGAGCTCTCCTTCCTTGCTTTGTGGTCATCTGCAAACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

432

Amino Acids

47.13

Weight (kDa)

9.4

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54630
fragaria_vesca FvH4_3g21630 FvH4_3g21630
malus_domestica MD03G1214000.v1.1 MD11G1229000.v1.1
prunus_persica Prupe.4G200600_v2.0.a1 Prupe.4G200600_v2.0.a1
pyrus_communis pycom11g20330
rosa_chinensis RchiOBHm_Chr5g0037081
rosa_laevigata RLG00000033750
rosa_multiflora Rmu_ssc0000260.1_g000002
rosa_roxburghii Rroxscaffold_1G00043870
rosa_rugosa Rorug05G0160300
rosa_samantha Rh5AG252400 Rh5BG254200 Rh5CG286800 Rh5DG262700
rosa_wichuraiana Rw5G023290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 905
AclWI GGATC 3 cut(s) 62, 989, 1085
AcoI YGGCCR 2 cut(s) 751, 1177
AcsI RAATTY 1 cut(s) 525
AcuI CTGAAG 2 cut(s) 44, 657
AfiI CCNNNNNNNGG 3 cut(s) 386, 518, 1101
AgsI TTSAA 7 cut(s) 132, 348, 733, 772, 1047, 1076, 1232
AjnI CCWGG 4 cut(s) 370, 379, 385, 511
AleI CACNNNNGTG 2 cut(s) 603, 911
AluBI AGCT 9 cut(s) 283, 506, 632, 726, 889, 1015, 1061, 1237, 1259
AluI AGCT 9 cut(s) 283, 506, 632, 726, 889, 1015, 1061, 1237, 1259
Alw21I GWGCWC 5 cut(s) 95, 285, 408, 1017, 1261
Alw26I GTCTC 3 cut(s) 169, 550, 953
Alw44I GTGCAC 1 cut(s) 404
AlwI GGATC 3 cut(s) 62, 989, 1085
Ama87I CYCGRG 2 cut(s) 271, 1254
AoxI GGCC 6 cut(s) 188, 368, 382, 751, 915, 1177
ApaLI GTGCAC 1 cut(s) 404
ApeKI GCWGC 2 cut(s) 204, 632
ApoI RAATTY 1 cut(s) 525
AspA2I CCTAGG 1 cut(s) 863
AspS9I GGNCC 2 cut(s) 383, 429
AsuHPI GGTGA 4 cut(s) 314, 620, 695, 838
AsuII TTCGAA 1 cut(s) 807
AvaI CYCGRG 2 cut(s) 271, 1254
AvaII GGWCC 1 cut(s) 429
AvrII CCTAGG 1 cut(s) 863
BaeGI GKGCMC 1 cut(s) 408
BanI GGYRCC 1 cut(s) 905
BanII GRGCYC 3 cut(s) 285, 1017, 1261
Bbv12I GWGCWC 5 cut(s) 95, 285, 408, 1017, 1261
BbvI GCAGC 2 cut(s) 216, 619
BccI CCATC 4 cut(s) 427, 654, 817, 872
BciT130I CCWGG 4 cut(s) 372, 381, 387, 513
BciVI GTATCC 1 cut(s) 352
BcoDI GTCTC 3 cut(s) 169, 550, 953
BfaI CTAG 2 cut(s) 693, 864
BfmI CTRYAG 4 cut(s) 138, 216, 471, 1120
BfuI GTATCC 1 cut(s) 352
BglII AGATCT 1 cut(s) 212
BisI GCNGC 2 cut(s) 205, 633
BlnI CCTAGG 1 cut(s) 863
BlsI GCNGC 2 cut(s) 206, 634
Bme1390I CCNGG 4 cut(s) 372, 381, 387, 513
Bme18I GGWCC 1 cut(s) 429
BmeT110I CYCGRG 2 cut(s) 271, 1254
BmgT120I GGNCC 2 cut(s) 383, 429
BmiI GGNNCC 1 cut(s) 907
BmrFI CCNGG 4 cut(s) 372, 381, 387, 513
BmrI ACTGGG 1 cut(s) 1194
BmsI GCATC 1 cut(s) 476
BmuI ACTGGG 1 cut(s) 1194
Bpu14I TTCGAA 1 cut(s) 807
BpuEI CTTGAG 3 cut(s) 134, 928, 1001
BsaI GGTCTC 1 cut(s) 550
BsaJI CCNNGG 5 cut(s) 298, 385, 446, 863, 925
Bsc4I CCNNNNNNNGG 3 cut(s) 386, 518, 1101
Bse118I RCCGGY 1 cut(s) 1175
Bse1I ACTGG 1 cut(s) 1189
Bse3DI GCAATG 1 cut(s) 870
BseBI CCWGG 4 cut(s) 372, 381, 387, 513
BseDI CCNNGG 5 cut(s) 298, 385, 446, 863, 925
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 3 cut(s) 386, 518, 1101
BseMI GCAATG 1 cut(s) 870
BseNI ACTGG 1 cut(s) 1189
BseRI GAGGAG 1 cut(s) 432
BseSI GKGCMC 1 cut(s) 408
BseXI GCAGC 2 cut(s) 216, 619
BseYI CCCAGC 1 cut(s) 1133
BshFI GGCC 6 cut(s) 190, 370, 384, 753, 917, 1179
BshNI GGYRCC 1 cut(s) 905
BsiHKAI GWGCWC 5 cut(s) 95, 285, 408, 1017, 1261
BsiHKCI CYCGRG 2 cut(s) 271, 1254
BsiSI CCGG 3 cut(s) 718, 754, 1176
BslI CCNNNNNNNGG 3 cut(s) 386, 518, 1101
BsmAI GTCTC 3 cut(s) 169, 550, 953
BsnI GGCC 6 cut(s) 190, 370, 384, 753, 917, 1179
Bso31I GGTCTC 1 cut(s) 550
BsoBI CYCGRG 2 cut(s) 271, 1254
Bsp119I TTCGAA 1 cut(s) 807
Bsp1286I GDGCHC 5 cut(s) 95, 285, 408, 1017, 1261
Bsp143I GATC 5 cut(s) 53, 67, 212, 981, 1090
BspANI GGCC 6 cut(s) 190, 370, 384, 753, 917, 1179
BspHI TCATGA 1 cut(s) 64
BspLI GGNNCC 1 cut(s) 907
BspMAI CTGCAG 2 cut(s) 475, 1124
BspPI GGATC 3 cut(s) 62, 989, 1085
BspQI GCTCTTC 2 cut(s) 1051, 1227
BspT104I TTCGAA 1 cut(s) 807
BspT107I GGYRCC 1 cut(s) 905
BspTNI GGTCTC 1 cut(s) 550
BsrDI GCAATG 1 cut(s) 870
BsrFI RCCGGY 1 cut(s) 1175
BsrI ACTGG 1 cut(s) 1189
BssAI RCCGGY 1 cut(s) 1175
BssECI CCNNGG 5 cut(s) 298, 385, 446, 863, 925
BssMI GATC 5 cut(s) 53, 67, 212, 981, 1090
BssT1I CCWWGG 4 cut(s) 298, 446, 863, 925
Bst2UI CCWGG 4 cut(s) 372, 381, 387, 513
Bst4CI ACNGT 4 cut(s) 10, 403, 559, 913
Bst6I CTCTTC 2 cut(s) 1051, 1227
BstBI TTCGAA 1 cut(s) 807
BstC8I GCNNGC 3 cut(s) 841, 919, 1177
BstDEI CTNAG 2 cut(s) 365, 852
BstENI CCTNNNNNAGG 1 cut(s) 1099
BstF5I GGATG 1 cut(s) 160
BstKTI GATC 5 cut(s) 56, 70, 215, 984, 1093
BstMAI GTCTC 3 cut(s) 169, 550, 953
BstMBI GATC 5 cut(s) 53, 67, 212, 981, 1090
BstMWI GCNNNNNNNGC 4 cut(s) 381, 638, 723, 1243
BstNI CCWGG 4 cut(s) 372, 381, 387, 513
BstNSI RCATGY 2 cut(s) 662, 787
BstSCI CCNGG 4 cut(s) 370, 379, 385, 511
BstSFI CTRYAG 4 cut(s) 138, 216, 471, 1120
BstSLI GKGCMC 1 cut(s) 408
BstV1I GCAGC 2 cut(s) 216, 619
BstX2I RGATCY 2 cut(s) 212, 1090
BstXI CCANNNNNNTGG 1 cut(s) 12
BstYI RGATCY 2 cut(s) 212, 1090
BsuI GTATCC 1 cut(s) 352
BsuRI GGCC 6 cut(s) 190, 370, 384, 753, 917, 1179
BtsCI GGATG 1 cut(s) 160
BtsI GCAGTG 2 cut(s) 37, 232
BtsIMutI CAGTG 5 cut(s) 15, 37, 232, 408, 909
Cac8I GCNNGC 3 cut(s) 841, 919, 1177
CciI TCATGA 1 cut(s) 64
Cfr10I RCCGGY 1 cut(s) 1175
Cfr13I GGNCC 2 cut(s) 383, 429
CspCI CAANNNNNGTGG 6 cut(s) 316, 319, 336, 351, 354, 371
CviAII CATG 6 cut(s) 65, 164, 251, 575, 659, 784
DdeI CTNAG 2 cut(s) 365, 852
DpnI GATC 5 cut(s) 55, 69, 214, 983, 1092
DpnII GATC 5 cut(s) 53, 67, 212, 981, 1090
EaeI YGGCCR 2 cut(s) 751, 1177
Eam1104I CTCTTC 2 cut(s) 1051, 1227
EarI CTCTTC 2 cut(s) 1051, 1227
Ecl136II GAGCTC 3 cut(s) 283, 1015, 1259
Eco130I CCWWGG 4 cut(s) 298, 446, 863, 925
Eco24I GRGCYC 3 cut(s) 285, 1017, 1261
Eco31I GGTCTC 1 cut(s) 550
Eco47I GGWCC 1 cut(s) 429
Eco53kI GAGCTC 3 cut(s) 283, 1015, 1259
Eco57I CTGAAG 2 cut(s) 44, 657
Eco88I CYCGRG 2 cut(s) 271, 1254
EcoICRI GAGCTC 3 cut(s) 283, 1015, 1259
EcoNI CCTNNNNNAGG 1 cut(s) 1099
EcoO109I RGGNCCY 1 cut(s) 429
EcoRI GAATTC 1 cut(s) 525
EcoRII CCWGG 4 cut(s) 370, 379, 385, 511
EcoT14I CCWWGG 4 cut(s) 298, 446, 863, 925
EcoT38I GRGCYC 3 cut(s) 285, 1017, 1261
ErhI CCWWGG 4 cut(s) 298, 446, 863, 925
FaeI CATG 6 cut(s) 68, 167, 254, 578, 662, 787
FatI CATG 6 cut(s) 64, 163, 250, 574, 658, 783
Fnu4HI GCNGC 2 cut(s) 205, 633
FokI GGATG 1 cut(s) 167
FriOI GRGCYC 3 cut(s) 285, 1017, 1261
Fsp4HI GCNGC 2 cut(s) 205, 633
FspBI CTAG 2 cut(s) 693, 864
GluI GCNGC 2 cut(s) 205, 633
GsaI CCCAGC 1 cut(s) 1137
HaeIII GGCC 6 cut(s) 190, 370, 384, 753, 917, 1179
HapII CCGG 3 cut(s) 718, 754, 1176
Hin1II CATG 6 cut(s) 68, 167, 254, 578, 662, 787
HinfI GANTC 5 cut(s) 160, 686, 804, 1067, 1193
HpaII CCGG 3 cut(s) 718, 754, 1176
HphI GGTGA 4 cut(s) 314, 620, 695, 838
Hpy166II GTNNAC 2 cut(s) 406, 778
Hpy188I TCNGA 7 cut(s) 24, 49, 58, 237, 534, 739, 961
Hpy188III TCNNGA 5 cut(s) 65, 100, 318, 693, 818
Hpy8I GTNNAC 2 cut(s) 406, 778
HpyAV CCTTC 6 cut(s) 299, 479, 674, 1003, 1016, 1274
HpyCH4III ACNGT 4 cut(s) 10, 403, 559, 913
HpyF10VI GCNNNNNNNGC 4 cut(s) 381, 638, 723, 1243
HpyF3I CTNAG 2 cut(s) 365, 852
Hsp92II CATG 6 cut(s) 68, 167, 254, 578, 662, 787
KroI GCCGGC 1 cut(s) 1175
KroNI GCCGGC 1 cut(s) 1177
Kzo9I GATC 5 cut(s) 53, 67, 212, 981, 1090
LguI GCTCTTC 2 cut(s) 1051, 1227
LmnI GCTCC 3 cut(s) 222, 926, 1012
Lsp1109I GCAGC 2 cut(s) 216, 619
LweI GCATC 1 cut(s) 476
MaeI CTAG 2 cut(s) 693, 864
MaeIII GTNAC 3 cut(s) 596, 664, 683
MalI GATC 5 cut(s) 55, 69, 214, 983, 1092
MboI GATC 5 cut(s) 53, 67, 212, 981, 1090
MboII GAAGA 7 cut(s) 37, 573, 821, 1068, 1097, 1100, 1244
MfeI CAATTG 1 cut(s) 745
MflI RGATCY 2 cut(s) 212, 1090
MhlI GDGCHC 5 cut(s) 95, 285, 408, 1017, 1261
MluCI AATT 8 cut(s) 199, 258, 309, 525, 699, 745, 1128, 1170
MlyI GAGTC 2 cut(s) 680, 1076
MroNI GCCGGC 1 cut(s) 1175
MseI TTAA 1 cut(s) 414
MslI CAYNNNNRTG 2 cut(s) 603, 911
MspI CCGG 3 cut(s) 718, 754, 1176
MspR9I CCNGG 4 cut(s) 372, 381, 387, 513
MunI CAATTG 1 cut(s) 745
MvaI CCWGG 4 cut(s) 372, 381, 387, 513
MwoI GCNNNNNNNGC 4 cut(s) 381, 638, 723, 1243
NaeI GCCGGC 1 cut(s) 1177
NdeII GATC 5 cut(s) 53, 67, 212, 981, 1090
NgoMIV GCCGGC 1 cut(s) 1175
NlaIII CATG 6 cut(s) 68, 167, 254, 578, 662, 787
NlaIV GGNNCC 1 cut(s) 907
NmuCI GTSAC 2 cut(s) 596, 683
NspI RCATGY 2 cut(s) 662, 787
NspV TTCGAA 1 cut(s) 807
OliI CACNNNNGTG 2 cut(s) 603, 911
PaeR7I CTCGAG 2 cut(s) 271, 1254
PagI TCATGA 1 cut(s) 64
PciSI GCTCTTC 2 cut(s) 1051, 1227
PdiI GCCGGC 1 cut(s) 1177
PfeI GAWTC 3 cut(s) 160, 804, 1193
PflFI GACNNNGTC 1 cut(s) 557
PkrI GCNGC 2 cut(s) 206, 634
PleI GAGTC 2 cut(s) 680, 1075
PpsI GAGTC 2 cut(s) 680, 1075
PpuMI RGGWCCY 1 cut(s) 429
Psp124BI GAGCTC 3 cut(s) 285, 1017, 1261
Psp5II RGGWCCY 1 cut(s) 429
Psp6I CCWGG 4 cut(s) 370, 379, 385, 511
PspFI CCCAGC 1 cut(s) 1133
PspGI CCWGG 4 cut(s) 370, 379, 385, 511
PspN4I GGNNCC 1 cut(s) 907
PspPI GGNCC 2 cut(s) 383, 429
PspPPI RGGWCCY 1 cut(s) 429
PspXI VCTCGAGB 2 cut(s) 271, 1254
PstI CTGCAG 2 cut(s) 475, 1124
PsuI RGATCY 2 cut(s) 212, 1090
PsyI GACNNNGTC 1 cut(s) 557
RseI CAYNNNNRTG 2 cut(s) 603, 911
SacI GAGCTC 3 cut(s) 285, 1017, 1261
SapI GCTCTTC 2 cut(s) 1051, 1227
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 2 cut(s) 205, 633
Sau3AI GATC 5 cut(s) 53, 67, 212, 981, 1090
Sau96I GGNCC 2 cut(s) 383, 429
SchI GAGTC 2 cut(s) 680, 1076
ScrFI CCNGG 4 cut(s) 372, 381, 387, 513
SduI GDGCHC 5 cut(s) 95, 285, 408, 1017, 1261
SfaNI GCATC 1 cut(s) 476
SfcI CTRYAG 4 cut(s) 138, 216, 471, 1120
Sfr274I CTCGAG 2 cut(s) 271, 1254
SfuI TTCGAA 1 cut(s) 807
SinI GGWCC 1 cut(s) 429
SlaI CTCGAG 2 cut(s) 271, 1254
SmiMI CAYNNNNRTG 2 cut(s) 603, 911
SmlI CTYRAG 5 cut(s) 149, 271, 943, 1016, 1254
SmoI CTYRAG 5 cut(s) 149, 271, 943, 1016, 1254
Sse9I AATT 8 cut(s) 199, 258, 309, 525, 699, 745, 1128, 1170
SspI AATATT 2 cut(s) 1080, 1228
SspMI CTAG 2 cut(s) 693, 864
SstI GAGCTC 3 cut(s) 285, 1017, 1261
StyD4I CCNGG 4 cut(s) 370, 379, 385, 511
StyI CCWWGG 4 cut(s) 298, 446, 863, 925
TaaI ACNGT 4 cut(s) 10, 403, 559, 913
TaqI TCGA 6 cut(s) 272, 705, 802, 807, 817, 1255
TasI AATT 8 cut(s) 199, 258, 309, 525, 699, 745, 1128, 1170
TfiI GAWTC 3 cut(s) 160, 804, 1193
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 5 cut(s) 15, 44, 232, 408, 916
TseFI GTSAC 2 cut(s) 596, 683
TseI GCWGC 2 cut(s) 204, 632
Tsp45I GTSAC 2 cut(s) 596, 683
TspDTI ATGAA 5 cut(s) 53, 152, 267, 848, 1133
TspRI CASTG 5 cut(s) 15, 44, 232, 408, 916
Tth111I GACNNNGTC 1 cut(s) 557
VneI GTGCAC 1 cut(s) 404
VpaK11BI GGWCC 1 cut(s) 429
XagI CCTNNNNNAGG 1 cut(s) 1099
XapI RAATTY 1 cut(s) 525
XbaI TCTAGA 1 cut(s) 692
XceI RCATGY 2 cut(s) 662, 787
XhoI CTCGAG 2 cut(s) 271, 1254
XmaJI CCTAGG 1 cut(s) 863
XspI CTAG 2 cut(s) 693, 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.