AT5G57170

macrophage migration inhibitory factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011457
Physical Location & Seq
Reverse (-)
23161877 .. 23163290
1414 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57170.2

Sequence Viewer

Length: 348 bp
ATGCCCACTTTGAATCTCTTCACTAACATACCAGTCGACGCCGTCACTTGCTCAGACATCCTCAAGGACGCCACTAAGGCCGTCGCTAAAATCATCGGCAAACCTGAATCCTATGTGATGATACTGCTTAACAGTGGAGTGCCCATTGCATTTGCCGGTACCGAGGAACCTGCTGCATATGGAGAATTGATATCTATTGGGGGATTAGGACCTGGCGTAAACGGGAAGCTTAGCGAGACGATATCTGAGATTCTCCAAATTAAGCTCTCCATAGACAGCTCTCGCTTTTATATCAAATTCTACGATTCTCCGCGACCTTTCTTCGGTTACAATGGATCAACTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

115

Amino Acids

12.32

Weight (kDa)

4.96

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 2 - 103 1.6e-20 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 178
Acc65I GGTACC 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 158
AccI GTMKAC 1 cut(s) 36
AccII CGCG 1 cut(s) 313
AciI CCGC 1 cut(s) 311
AclWI GGATC 1 cut(s) 343
AcsI RAATTY 1 cut(s) 296
AcyI GRCGYC 2 cut(s) 39, 69
AfaI GTAC 1 cut(s) 160
AfiI CCNNNNNNNGG 1 cut(s) 323
AgsI TTSAA 1 cut(s) 13
AjnI CCWGG 1 cut(s) 211
AluBI AGCT 3 cut(s) 229, 265, 279
AluI AGCT 3 cut(s) 229, 265, 279
Alw26I GTCTC 1 cut(s) 230
AlwI GGATC 1 cut(s) 343
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 1 cut(s) 173
ApoI RAATTY 1 cut(s) 296
Asp700I GAANNNNTTC 1 cut(s) 17
Asp718I GGTACC 1 cut(s) 158
AspS9I GGNCC 1 cut(s) 209
AvaII GGWCC 1 cut(s) 209
BaeGI GKGCMC 1 cut(s) 144
BanI GGYRCC 1 cut(s) 158
BbvI GCAGC 1 cut(s) 160
BceAI ACGGC 2 cut(s) 26, 65
BcgI CGANNNNNNTGC 2 cut(s) 152, 186
BciT130I CCWGG 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 230
BfuAI ACCTGC 1 cut(s) 178
BglI GCCNNNNNGGC 1 cut(s) 77
BisI GCNGC 1 cut(s) 174
BlpI GCTNAGC 1 cut(s) 230
BlsI GCNGC 1 cut(s) 175
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 2 cut(s) 160, 168
BmrFI CCNGG 1 cut(s) 213
Bpu1102I GCTNAGC 1 cut(s) 230
BpuEI CTTGAG 1 cut(s) 47
BsaHI GRCGYC 2 cut(s) 39, 69
BsaJI CCNNGG 1 cut(s) 162
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 32
Bse3DI GCAATG 1 cut(s) 144
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 162
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 323
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 2 cut(s) 66, 237
BseNI ACTGG 1 cut(s) 32
BseSI GKGCMC 1 cut(s) 144
BseXI GCAGC 1 cut(s) 160
Bsh1236I CGCG 1 cut(s) 313
BshFI GGCC 1 cut(s) 80
BshNI GGYRCC 1 cut(s) 158
BsiSI CCGG 1 cut(s) 156
BslI CCNNNNNNNGG 1 cut(s) 323
BsmAI GTCTC 1 cut(s) 230
BsmBI CGTCTC 1 cut(s) 230
BsnI GGCC 1 cut(s) 80
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 1 cut(s) 335
Bsp1720I GCTNAGC 1 cut(s) 230
BspACI CCGC 1 cut(s) 311
BspANI GGCC 1 cut(s) 80
BspCNI CTCAG 2 cut(s) 65, 238
BspFNI CGCG 1 cut(s) 313
BspLI GGNNCC 2 cut(s) 160, 168
BspMI ACCTGC 1 cut(s) 178
BspPI GGATC 1 cut(s) 343
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 144
BsrFI RCCGGY 1 cut(s) 155
BsrI ACTGG 1 cut(s) 32
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 1 cut(s) 335
BssNI GRCGYC 2 cut(s) 39, 69
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 1 cut(s) 134
Bst6I CTCTTC 1 cut(s) 23
BstACI GRCGYC 2 cut(s) 39, 69
BstDEI CTNAG 4 cut(s) 52, 75, 230, 246
BstF5I GGATG 1 cut(s) 57
BstFNI CGCG 1 cut(s) 313
BstKTI GATC 1 cut(s) 338
BstMAI GTCTC 1 cut(s) 230
BstMBI GATC 1 cut(s) 335
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstNI CCWGG 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 211
BstSLI GKGCMC 1 cut(s) 144
BstUI CGCG 1 cut(s) 313
BstV1I GCAGC 1 cut(s) 160
BsuRI GGCC 1 cut(s) 80
BtsCI GGATG 1 cut(s) 57
BtsIMutI CAGTG 1 cut(s) 139
BveI ACCTGC 1 cut(s) 178
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 209
CseI GACGC 2 cut(s) 47, 77
Csp6I GTAC 1 cut(s) 159
CviJI RGCY 4 cut(s) 80, 229, 265, 279
CviKI_1 RGCY 4 cut(s) 80, 229, 265, 279
CviQI GTAC 1 cut(s) 159
DdeI CTNAG 4 cut(s) 52, 75, 230, 246
DpnI GATC 1 cut(s) 337
DpnII GATC 1 cut(s) 335
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
Eco32I GATATC 2 cut(s) 192, 243
Eco47I GGWCC 1 cut(s) 209
EcoO109I RGGNCCY 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 211
EcoRV GATATC 2 cut(s) 192, 243
Esp3I CGTCTC 1 cut(s) 230
FaiI YATR 6 cut(s) 29, 114, 178, 180, 272, 291
FauNDI CATATG 1 cut(s) 178
FblI GTMKAC 1 cut(s) 36
Fnu4HI GCNGC 1 cut(s) 174
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 1 cut(s) 174
GluI GCNGC 1 cut(s) 174
HaeIII GGCC 1 cut(s) 80
HapII CCGG 1 cut(s) 156
HgaI GACGC 2 cut(s) 47, 77
Hin1I GRCGYC 2 cut(s) 39, 69
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 4 cut(s) 13, 107, 250, 305
HpaII CCGG 1 cut(s) 156
Hpy166II GTNNAC 2 cut(s) 37, 220
Hpy188I TCNGA 3 cut(s) 55, 247, 347
Hpy8I GTNNAC 2 cut(s) 37, 220
Hpy99I CGWCG 2 cut(s) 41, 86
HpyCH4III ACNGT 1 cut(s) 134
HpyCH4V TGCA 2 cut(s) 149, 176
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpyF3I CTNAG 4 cut(s) 52, 75, 230, 246
Hsp92I GRCGYC 2 cut(s) 39, 69
KpnI GGTACC 1 cut(s) 162
Kzo9I GATC 1 cut(s) 335
LpnPI CCDG 6 cut(s) 45, 117, 169, 183, 198, 225
Lsp1109I GCAGC 1 cut(s) 160
MaeIII GTNAC 2 cut(s) 43, 326
MalI GATC 1 cut(s) 337
MboI GATC 1 cut(s) 335
MboII GAAGA 2 cut(s) 10, 313
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 3 cut(s) 185, 258, 296
MnlI CCTC 2 cut(s) 71, 157
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 2 cut(s) 129, 261
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 213
MvnI CGCG 1 cut(s) 313
MwoI GCNNNNNNNGC 1 cut(s) 77
NdeI CATATG 1 cut(s) 178
NdeII GATC 1 cut(s) 335
NlaIV GGNNCC 2 cut(s) 160, 168
NmuCI GTSAC 1 cut(s) 43
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 4 cut(s) 13, 107, 250, 305
PflFI GACNNNGTC 1 cut(s) 41
PkrI GCNGC 1 cut(s) 175
PpuMI RGGWCCY 1 cut(s) 209
Psp5II RGGWCCY 1 cut(s) 209
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 2 cut(s) 160, 168
PspPI GGNCC 1 cut(s) 209
PspPPI RGGWCCY 1 cut(s) 209
PsyI GACNNNGTC 1 cut(s) 41
RsaI GTAC 1 cut(s) 160
RsaNI GTAC 1 cut(s) 159
SalI GTCGAC 1 cut(s) 35
SaqAI TTAA 2 cut(s) 129, 261
SatI GCNGC 1 cut(s) 174
Sau3AI GATC 1 cut(s) 335
Sau96I GGNCC 1 cut(s) 209
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 1 cut(s) 144
SetI ASST 7 cut(s) 106, 172, 214, 231, 267, 281, 319
SinI GGWCC 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 3 cut(s) 185, 258, 296
SsiI CCGC 1 cut(s) 311
StyD4I CCNGG 1 cut(s) 211
TaaI ACNGT 1 cut(s) 134
TaqI TCGA 1 cut(s) 36
TasI AATT 3 cut(s) 185, 258, 296
TfiI GAWTC 4 cut(s) 13, 107, 250, 305
Tru1I TTAA 2 cut(s) 129, 261
Tru9I TTAA 2 cut(s) 129, 261
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 1 cut(s) 173
Tsp45I GTSAC 1 cut(s) 43
TspRI CASTG 1 cut(s) 139
Tth111I GACNNNGTC 1 cut(s) 41
VpaK11BI GGWCC 1 cut(s) 209
XapI RAATTY 1 cut(s) 296
XmiI GTMKAC 1 cut(s) 36
XmnI GAANNNNTTC 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.