pycom13g21330

Macrophage migration inhibitory factor homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011457
Physical Location & Seq
Reverse (-)
17873752 .. 17874763
1012 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g21330.1

Sequence Viewer

Length: 345 bp
ATGCCGACGTTGAATCTGTTTACCAACTTGCCGGTGGACGGAGTTGTGGCCTCTGACATCCTCAAGGACGCCACCAAAGCCGTTTCTAAGATCATTGGCAAACCCGAGTCCTATGTGATGATTTTGCTCAACGGGTCTGTGCCAATTGCATTTGCTGGCACCGAAGAGCCTGCTGCGTATGGAGAATTGATCTCCATTGGGGGCATTGGACCAGGTGTTAACGGAAAACTGAGTTCAACCATTGCAGAAATTCTTGAAACTAAGCTCTCCATTGATAGCTCCCGCTTCTATATCAAATTTTATGACGTTGAGCGGTCCTTCGGGTTCAACGGCTCAACATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

115

Amino Acids

12.09

Weight (kDa)

4.79

Isoelectric Point (pI)

41.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 2 - 114 4e-23 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 158
AccBSI CCGCTC 1 cut(s) 313
AciI CCGC 2 cut(s) 283, 313
AcsI RAATTY 2 cut(s) 249, 296
AcyI GRCGYC 1 cut(s) 69
AfiI CCNNNNNNNGG 1 cut(s) 38
AgsI TTSAA 4 cut(s) 13, 237, 257, 328
AjnI CCWGG 1 cut(s) 211
AluBI AGCT 2 cut(s) 265, 279
AluI AGCT 2 cut(s) 265, 279
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 1 cut(s) 173
ApoI RAATTY 2 cut(s) 249, 296
AspS9I GGNCC 2 cut(s) 209, 315
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 2 cut(s) 209, 315
BanI GGYRCC 1 cut(s) 158
BbvI GCAGC 1 cut(s) 160
BceAI ACGGC 1 cut(s) 65
BcgI CGANNNNNNTGC 2 cut(s) 152, 186
BciT130I CCWGG 1 cut(s) 213
BisI GCNGC 1 cut(s) 174
BlsI GCNGC 1 cut(s) 175
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 2 cut(s) 209, 315
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 2 cut(s) 209, 315
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 47
BsaHI GRCGYC 1 cut(s) 69
Bsc4I CCNNNNNNNGG 1 cut(s) 38
Bse118I RCCGGY 1 cut(s) 31
Bse3DI GCAATG 1 cut(s) 240
BseBI CCWGG 1 cut(s) 213
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 38
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 221
BseXI GCAGC 1 cut(s) 160
BshFI GGCC 1 cut(s) 50
BshNI GGYRCC 1 cut(s) 158
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 38
BsnI GGCC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 104
Bsp143I GATC 2 cut(s) 90, 189
BspACI CCGC 2 cut(s) 283, 313
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 1 cut(s) 222
BspLI GGNNCC 1 cut(s) 160
BspQI GCTCTTC 1 cut(s) 159
BspT107I GGYRCC 1 cut(s) 158
BsrBI CCGCTC 1 cut(s) 313
BsrDI GCAATG 1 cut(s) 240
BsrFI RCCGGY 1 cut(s) 31
BssAI RCCGGY 1 cut(s) 31
BssMI GATC 2 cut(s) 90, 189
BssNI GRCGYC 1 cut(s) 69
Bst2UI CCWGG 1 cut(s) 213
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 69
BstC8I GCNNGC 2 cut(s) 157, 171
BstDEI CTNAG 3 cut(s) 87, 230, 261
BstF5I GGATG 1 cut(s) 57
BstKTI GATC 2 cut(s) 93, 192
BstMBI GATC 2 cut(s) 90, 189
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstNI CCWGG 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 211
BstV1I GCAGC 1 cut(s) 160
BsuRI GGCC 1 cut(s) 50
BtsCI GGATG 1 cut(s) 57
Cac8I GCNNGC 2 cut(s) 157, 171
Cfr10I RCCGGY 1 cut(s) 31
Cfr13I GGNCC 2 cut(s) 209, 315
CseI GACGC 1 cut(s) 77
CsiI ACCWGGT 1 cut(s) 211
CviJI RGCY 6 cut(s) 50, 80, 169, 265, 279, 333
CviKI_1 RGCY 6 cut(s) 50, 80, 169, 265, 279, 333
DdeI CTNAG 3 cut(s) 87, 230, 261
DpnI GATC 2 cut(s) 92, 191
DpnII GATC 2 cut(s) 90, 189
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco47I GGWCC 2 cut(s) 209, 315
Eco88I CYCGRG 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 211
FaiI YATR 4 cut(s) 114, 180, 291, 303
FauI CCCGC 1 cut(s) 290
Fnu4HI GCNGC 1 cut(s) 174
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 1 cut(s) 174
GluI GCNGC 1 cut(s) 174
HaeIII GGCC 1 cut(s) 50
HapII CCGG 1 cut(s) 32
HgaI GACGC 1 cut(s) 77
Hin1I GRCGYC 1 cut(s) 69
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 2 cut(s) 13, 107
HpaI GTTAAC 1 cut(s) 220
HpaII CCGG 1 cut(s) 32
Hpy166II GTNNAC 3 cut(s) 21, 37, 220
Hpy188I TCNGA 1 cut(s) 55
Hpy188III TCNNGA 1 cut(s) 254
Hpy8I GTNNAC 3 cut(s) 21, 37, 220
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 328
HpyCH4IV ACGT 2 cut(s) 8, 306
HpyCH4V TGCA 2 cut(s) 149, 245
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpyF3I CTNAG 3 cut(s) 87, 230, 261
HpySE526I ACGT 2 cut(s) 8, 306
Hsp92I GRCGYC 1 cut(s) 69
KspAI GTTAAC 1 cut(s) 220
Kzo9I GATC 2 cut(s) 90, 189
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 1 cut(s) 284
LpnPI CCDG 5 cut(s) 45, 141, 183, 198, 225
Lsp1109I GCAGC 1 cut(s) 160
MabI ACCWGGT 1 cut(s) 211
MaeII ACGT 2 cut(s) 8, 306
MalI GATC 2 cut(s) 92, 191
MbiI CCGCTC 1 cut(s) 313
MboI GATC 2 cut(s) 90, 189
MboII GAAGA 1 cut(s) 176
MfeI CAATTG 1 cut(s) 144
MluCI AATT 4 cut(s) 144, 185, 249, 296
MlyI GAGTC 1 cut(s) 116
MnlI CCTC 2 cut(s) 61, 71
MseI TTAA 1 cut(s) 219
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 1 cut(s) 213
MunI CAATTG 1 cut(s) 144
MvaI CCWGG 1 cut(s) 213
MwoI GCNNNNNNNGC 1 cut(s) 77
NdeII GATC 2 cut(s) 90, 189
NlaIV GGNNCC 1 cut(s) 160
PciSI GCTCTTC 1 cut(s) 159
PfeI GAWTC 1 cut(s) 13
PkrI GCNGC 1 cut(s) 175
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 2 cut(s) 209, 315
SapI GCTCTTC 1 cut(s) 159
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 1 cut(s) 174
Sau3AI GATC 2 cut(s) 90, 189
Sau96I GGNCC 2 cut(s) 209, 315
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 1 cut(s) 213
SetI ASST 5 cut(s) 11, 217, 267, 281, 309
SexAI ACCWGGT 1 cut(s) 211
SinI GGWCC 2 cut(s) 209, 315
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 4 cut(s) 144, 185, 249, 296
SsiI CCGC 2 cut(s) 283, 313
StyD4I CCNGG 1 cut(s) 211
TaiI ACGT 2 cut(s) 11, 309
TasI AATT 4 cut(s) 144, 185, 249, 296
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TseI GCWGC 1 cut(s) 173
TspGWI ACGGA 2 cut(s) 54, 237
VpaK11BI GGWCC 2 cut(s) 209, 315
XapI RAATTY 2 cut(s) 249, 296
XcmI CCANNNNNNNNNTGG 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.