Prupe.1G074700_v2.0.a1

Macrophage migration inhibitory factor homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0011457
Physical Location & Seq
Reverse (-)
5362774 .. 5365595
2822 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G074700.2

Sequence Viewer

Length: 369 bp
ATGCCGACGTTGAATTTGTTCACAAATCTGCCAGTGGATGCAGTGGTGGCCTCTGACATTCTCAAGGACGCCACCAAAGCCGTTTCTAAAATCATTGGCAAACCCGAGTCTTATGTGATGATTTTGCTGAACGGGTCAGTGCCTATGGCATTTGCGGGCACGGAAGAACCGGCAGCCTACGGAGAATTAATCTCCATTGGGGGCATTGGACCGAGTGTTAATGGAAAACTGAGTTCAACTATTGCAGAAATTCTGGAAACCAAGCTTTCCATCGATAGCTCCCGCTTCTATATCAAATTTTATGACGTTGAGAGTGTAGAAGGGTCACGGCCACTTCGGGCTGAAGTGTGGCTCCTCACTTGGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

123

Amino Acids

13.12

Weight (kDa)

4.73

Isoelectric Point (pI)

44.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 155, 283
AcoI YGGCCR 1 cut(s) 329
AcsI RAATTY 3 cut(s) 13, 249, 296
AcuI CTGAAG 1 cut(s) 363
AcyI GRCGYC 1 cut(s) 69
AgsI TTSAA 2 cut(s) 13, 237
AluBI AGCT 3 cut(s) 265, 279, 366
AluI AGCT 3 cut(s) 265, 279, 366
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 2 cut(s) 48, 329
ApeKI GCWGC 1 cut(s) 173
ApoI RAATTY 3 cut(s) 13, 249, 296
ArsI GACNNNNNNTTYG 1 cut(s) 30
AseI ATTAAT 1 cut(s) 188
Asp700I GAANNNNTTC 1 cut(s) 17
AspS9I GGNCC 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 209
BaeGI GKGCMC 1 cut(s) 161
BbvI GCAGC 1 cut(s) 185
BccI CCATC 1 cut(s) 278
BceAI ACGGC 2 cut(s) 65, 344
BisI GCNGC 1 cut(s) 174
BlsI GCNGC 1 cut(s) 175
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 353
BmsI GCATC 1 cut(s) 28
BpuEI CTTGAG 1 cut(s) 47
Bsa29I ATCGAT 1 cut(s) 273
BsaHI GRCGYC 1 cut(s) 69
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 1 cut(s) 32
BseCI ATCGAT 1 cut(s) 273
BseGI GGATG 1 cut(s) 43
BseMII CTCAG 1 cut(s) 221
BseNI ACTGG 1 cut(s) 32
BseRI GAGGAG 1 cut(s) 344
BseSI GKGCMC 1 cut(s) 161
BseXI GCAGC 1 cut(s) 185
BshFI GGCC 2 cut(s) 50, 331
BshVI ATCGAT 1 cut(s) 273
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 1 cut(s) 170
BsnI GGCC 2 cut(s) 50, 331
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 161
BspACI CCGC 2 cut(s) 155, 283
BspANI GGCC 2 cut(s) 50, 331
BspCNI CTCAG 1 cut(s) 222
BspDI ATCGAT 1 cut(s) 273
BspLI GGNNCC 1 cut(s) 353
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 1 cut(s) 32
BssAI RCCGGY 1 cut(s) 169
BssNI GRCGYC 1 cut(s) 69
BstACI GRCGYC 1 cut(s) 69
BstC8I GCNNGC 1 cut(s) 157
BstDEI CTNAG 1 cut(s) 230
BstF5I GGATG 1 cut(s) 43
BstMWI GCNNNNNNNGC 2 cut(s) 47, 77
BstSLI GKGCMC 1 cut(s) 161
BstV1I GCAGC 1 cut(s) 185
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 2 cut(s) 50, 331
BsuTUI ATCGAT 1 cut(s) 273
BtsCI GGATG 1 cut(s) 43
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 3 cut(s) 39, 48, 144
Cac8I GCNNGC 1 cut(s) 157
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 209
ClaI ATCGAT 1 cut(s) 273
CseI GACGC 1 cut(s) 77
CviJI RGCY 9 cut(s) 50, 80, 176, 265, 279, 331, 341, 352, 366
CviKI_1 RGCY 9 cut(s) 50, 80, 176, 265, 279, 331, 341, 352, 366
DdeI CTNAG 1 cut(s) 230
EaeI YGGCCR 1 cut(s) 329
Eco47I GGWCC 1 cut(s) 209
Eco57I CTGAAG 1 cut(s) 363
Eco88I CYCGRG 1 cut(s) 104
FaiI YATR 4 cut(s) 114, 146, 291, 303
FauI CCCGC 2 cut(s) 148, 290
Fnu4HI GCNGC 1 cut(s) 174
FokI GGATG 1 cut(s) 50
Fsp4HI GCNGC 1 cut(s) 174
GluI GCNGC 1 cut(s) 174
HaeIII GGCC 2 cut(s) 50, 331
HapII CCGG 1 cut(s) 170
HgaI GACGC 1 cut(s) 77
Hin1I GRCGYC 1 cut(s) 69
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 1 cut(s) 107
HpaII CCGG 1 cut(s) 170
Hpy166II GTNNAC 1 cut(s) 21
Hpy188I TCNGA 1 cut(s) 55
Hpy188III TCNNGA 1 cut(s) 254
Hpy8I GTNNAC 1 cut(s) 21
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 314
HpyCH4IV ACGT 2 cut(s) 8, 306
HpyCH4V TGCA 2 cut(s) 41, 245
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 77
HpyF3I CTNAG 1 cut(s) 230
HpySE526I ACGT 2 cut(s) 8, 306
Hsp92I GRCGYC 1 cut(s) 69
LmnI GCTCC 3 cut(s) 284, 357, 363
LpnPI CCDG 3 cut(s) 45, 183, 239
Lsp1109I GCAGC 1 cut(s) 185
LweI GCATC 1 cut(s) 28
MaeII ACGT 2 cut(s) 8, 306
MaeIII GTNAC 1 cut(s) 324
MboII GAAGA 1 cut(s) 176
MhlI GDGCHC 1 cut(s) 161
MluCI AATT 4 cut(s) 13, 185, 249, 296
MlyI GAGTC 1 cut(s) 116
MnlI CCTC 2 cut(s) 61, 365
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 2 cut(s) 188, 219
MspI CCGG 1 cut(s) 170
MwoI GCNNNNNNNGC 2 cut(s) 47, 77
NlaIV GGNNCC 1 cut(s) 353
NmuCI GTSAC 1 cut(s) 324
PdmI GAANNNNTTC 1 cut(s) 17
PkrI GCNGC 1 cut(s) 175
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
PshBI ATTAAT 1 cut(s) 188
PspN4I GGNNCC 1 cut(s) 353
PspPI GGNCC 1 cut(s) 209
SaqAI TTAA 2 cut(s) 188, 219
SatI GCNGC 1 cut(s) 174
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 1 cut(s) 116
SduI GDGCHC 1 cut(s) 161
SetI ASST 5 cut(s) 11, 267, 281, 309, 368
SfaNI GCATC 1 cut(s) 28
SinI GGWCC 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 4 cut(s) 13, 185, 249, 296
SsiI CCGC 2 cut(s) 155, 283
TaiI ACGT 2 cut(s) 11, 309
TaqI TCGA 1 cut(s) 273
TaqII GACCGA 1 cut(s) 226
TasI AATT 4 cut(s) 13, 185, 249, 296
Tru1I TTAA 2 cut(s) 188, 219
Tru9I TTAA 2 cut(s) 188, 219
TscAI CASTG 3 cut(s) 39, 48, 144
TseFI GTSAC 1 cut(s) 324
TseI GCWGC 1 cut(s) 173
Tsp45I GTSAC 1 cut(s) 324
TspGWI ACGGA 2 cut(s) 176, 195
TspRI CASTG 3 cut(s) 39, 48, 144
VpaK11BI GGWCC 1 cut(s) 209
VspI ATTAAT 1 cut(s) 188
XapI RAATTY 3 cut(s) 13, 249, 296
XmnI GAANNNNTTC 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.