MD16G1248200.v1.1

Macrophage migration inhibitory factor homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011457
Physical Location & Seq
Reverse (-)
27715916 .. 27717741
1826 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1248200.v1.1.491

Sequence Viewer

Length: 327 bp
ATGCCGACATTGAATCTGTTTACGGACGTGCCGGTGGACGGAGTGGTGGCCTCTGACATCCTCAAGGACGCCACCAAAGCCGTTTCTAAAATCATTGGCAAACCCGAGTCCTATGTGATGATTTTGCTCAACGGGACTCCGTCGATTGCATTTGCCAGTACAGAAGAGCCAGCTGTGTATGGAGAACTGATCTCCATTGGGGGCATTGGACCGGGTGTTAACGGCAAACTGAGTTCAACCATTGCAGAAGTTCTGGAAACTAAGCTCTCCATTGATAGCTCCCGCTTGTATGTCAAATTTTACGACGTTGAGGGGGAAGAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

109

Amino Acids

11.42

Weight (kDa)

4.49

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 2 - 105 4.5e-18 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 283
AcsI RAATTY 1 cut(s) 296
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 1 cut(s) 160
AfiI CCNNNNNNNGG 1 cut(s) 38
AgsI TTSAA 2 cut(s) 13, 237
AjiI CACGTC 1 cut(s) 28
AluBI AGCT 3 cut(s) 173, 265, 279
AluI AGCT 3 cut(s) 173, 265, 279
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 1 cut(s) 48
ApoI RAATTY 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 209
AsuC2I CCSGG 1 cut(s) 213
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 209
BceAI ACGGC 2 cut(s) 65, 238
BcnI CCSGG 1 cut(s) 213
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 104
BmgBI CACGTC 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 209
BmrFI CCNGG 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 213
BsaHI GRCGYC 1 cut(s) 69
Bsc4I CCNNNNNNNGG 1 cut(s) 38
Bse118I RCCGGY 1 cut(s) 31
Bse1I ACTGG 1 cut(s) 156
Bse3DI GCAATG 1 cut(s) 240
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 38
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 221
BseNI ACTGG 1 cut(s) 156
BshFI GGCC 1 cut(s) 50
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 2 cut(s) 32, 212
BslFI GGGAC 1 cut(s) 148
BslI CCNNNNNNNGG 1 cut(s) 38
BsmFI GGGAC 1 cut(s) 148
BsnI GGCC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 104
Bsp143I GATC 1 cut(s) 189
BspACI CCGC 1 cut(s) 283
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 1 cut(s) 222
BspQI GCTCTTC 1 cut(s) 159
BsrDI GCAATG 1 cut(s) 240
BsrFI RCCGGY 1 cut(s) 31
BsrI ACTGG 1 cut(s) 156
BssAI RCCGGY 1 cut(s) 31
BssMI GATC 1 cut(s) 189
BssNI GRCGYC 1 cut(s) 69
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 69
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 2 cut(s) 230, 261
BstF5I GGATG 1 cut(s) 57
BstKTI GATC 1 cut(s) 192
BstMBI GATC 1 cut(s) 189
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 211
BsuRI GGCC 1 cut(s) 50
BtrI CACGTC 1 cut(s) 28
BtsCI GGATG 1 cut(s) 57
Cac8I GCNNGC 1 cut(s) 171
Cfr10I RCCGGY 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 209
CseI GACGC 1 cut(s) 77
Csp6I GTAC 1 cut(s) 159
CviJI RGCY 6 cut(s) 50, 80, 169, 173, 265, 279
CviKI_1 RGCY 6 cut(s) 50, 80, 169, 173, 265, 279
CviQI GTAC 1 cut(s) 159
DdeI CTNAG 2 cut(s) 230, 261
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 104
FaiI YATR 3 cut(s) 114, 180, 291
FaqI GGGAC 1 cut(s) 148
FauI CCCGC 1 cut(s) 290
FokI GGATG 1 cut(s) 44
HaeIII GGCC 1 cut(s) 50
HapII CCGG 2 cut(s) 32, 212
HgaI GACGC 1 cut(s) 77
Hin1I GRCGYC 1 cut(s) 69
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 3 cut(s) 13, 107, 136
HpaI GTTAAC 1 cut(s) 220
HpaII CCGG 2 cut(s) 32, 212
Hpy166II GTNNAC 3 cut(s) 21, 37, 220
Hpy188I TCNGA 1 cut(s) 55
Hpy188III TCNNGA 1 cut(s) 254
Hpy8I GTNNAC 3 cut(s) 21, 37, 220
Hpy99I CGWCG 2 cut(s) 145, 308
HpyCH4IV ACGT 2 cut(s) 27, 306
HpyCH4V TGCA 2 cut(s) 149, 245
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpyF3I CTNAG 2 cut(s) 230, 261
HpySE526I ACGT 2 cut(s) 27, 306
Hsp92I GRCGYC 1 cut(s) 69
KspAI GTTAAC 1 cut(s) 220
Kzo9I GATC 1 cut(s) 189
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 1 cut(s) 284
LpnPI CCDG 5 cut(s) 45, 169, 183, 225, 239
MaeII ACGT 2 cut(s) 27, 306
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MboII GAAGA 1 cut(s) 176
MluCI AATT 1 cut(s) 296
MlyI GAGTC 2 cut(s) 116, 130
MnlI CCTC 3 cut(s) 61, 71, 304
MseI TTAA 1 cut(s) 219
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 2 cut(s) 32, 212
MspR9I CCNGG 1 cut(s) 213
MwoI GCNNNNNNNGC 1 cut(s) 77
NciI CCSGG 1 cut(s) 213
NdeII GATC 1 cut(s) 189
PciSI GCTCTTC 1 cut(s) 159
PfeI GAWTC 1 cut(s) 13
PflFI GACNNNGTC 1 cut(s) 139
PleI GAGTC 2 cut(s) 115, 130
PpsI GAGTC 2 cut(s) 115, 130
PspPI GGNCC 1 cut(s) 209
PsyI GACNNNGTC 1 cut(s) 139
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 1 cut(s) 160
RsaNI GTAC 1 cut(s) 159
SapI GCTCTTC 1 cut(s) 159
SaqAI TTAA 1 cut(s) 219
Sau3AI GATC 1 cut(s) 189
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 2 cut(s) 116, 130
ScrFI CCNGG 1 cut(s) 213
SetI ASST 5 cut(s) 30, 175, 267, 281, 309
SinI GGWCC 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 1 cut(s) 296
SsiI CCGC 1 cut(s) 283
StyD4I CCNGG 1 cut(s) 211
TaiI ACGT 2 cut(s) 30, 309
TaqI TCGA 1 cut(s) 143
TasI AATT 1 cut(s) 296
TatI WGTACW 1 cut(s) 158
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TspGWI ACGGA 3 cut(s) 38, 54, 129
Tth111I GACNNNGTC 1 cut(s) 139
VpaK11BI GGWCC 1 cut(s) 209
XapI RAATTY 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.