FvH4_4g07201

Macrophage migration inhibitory factor homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011457
Physical Location & Seq
Reverse (-)
6627500 .. 6628468
969 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g07201.t1

Sequence Viewer

Length: 348 bp
ATGCCGACGCTGAACCTGTTCACGAATGTTCCGGTCGACGCAGTGATAGTCTCCGACATTCTCAGGGACGCAACCAAAGCCGTCGCCAAAATCATTGGCAAACCCGAGTCCTATGTGATGATTTTGCTCAATGGGAGCGTGCCGATTGCGTTTGCGGGCACGGAAGAGCCGGCTGCGTATGGGGAATTGATCTCCATTGGGGGCATTGGACCCAGTGTTAATGGGAAGCTGAGTTCAACTATTGCAGAGATTCTGGAAACCAAGCTTTCCATAGAAAGCTCTCGCTTCTACATCAAATTCTATGACGTTCAGCGGCCATTCTTTGGCTTTAATGGCTCAACATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

116

Amino Acids

12.35

Weight (kDa)

4.99

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 2 - 115 3.6e-24 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 323
AccI GTMKAC 1 cut(s) 36
AciI CCGC 2 cut(s) 155, 313
AcoI YGGCCR 1 cut(s) 314
AcsI RAATTY 1 cut(s) 296
AfiI CCNNNNNNNGG 1 cut(s) 323
AgsI TTSAA 1 cut(s) 237
AloI GAACNNNNNNTCC 2 cut(s) 217, 249
AluBI AGCT 3 cut(s) 229, 265, 279
AluI AGCT 3 cut(s) 229, 265, 279
Alw26I GTCTC 1 cut(s) 55
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 1 cut(s) 314
ApeKI GCWGC 1 cut(s) 173
ApoI RAATTY 1 cut(s) 296
Asp700I GAANNNNTTC 1 cut(s) 17
AspS9I GGNCC 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 209
BaeGI GKGCMC 1 cut(s) 161
BbvI GCAGC 1 cut(s) 160
BceAI ACGGC 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 55
BisI GCNGC 2 cut(s) 174, 314
BlsI GCNGC 2 cut(s) 175, 315
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 211
BmrI ACTGGG 1 cut(s) 207
BmuI ACTGGG 1 cut(s) 207
BsaWI WCCGGW 1 cut(s) 31
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 1 cut(s) 213
BseLI CCNNNNNNNGG 1 cut(s) 323
BseMII CTCAG 2 cut(s) 76, 221
BseNI ACTGG 1 cut(s) 213
BseSI GKGCMC 1 cut(s) 161
BseXI GCAGC 1 cut(s) 160
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 1 cut(s) 316
BsiEI CGRYCG 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 2 cut(s) 32, 170
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 1 cut(s) 323
BsmAI GTCTC 1 cut(s) 55
BsmFI GGGAC 1 cut(s) 80
BsnI GGCC 1 cut(s) 316
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 161
Bsp143I GATC 1 cut(s) 189
BspACI CCGC 2 cut(s) 155, 313
BspANI GGCC 1 cut(s) 316
BspCNI CTCAG 2 cut(s) 75, 222
BspLI GGNNCC 1 cut(s) 211
BspQI GCTCTTC 1 cut(s) 159
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 1 cut(s) 213
BssAI RCCGGY 1 cut(s) 169
BssMI GATC 1 cut(s) 189
Bst6I CTCTTC 1 cut(s) 159
BstC8I GCNNGC 3 cut(s) 140, 157, 171
BstDEI CTNAG 2 cut(s) 62, 230
BstKTI GATC 1 cut(s) 192
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 1 cut(s) 189
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 2 cut(s) 77, 333
BstSLI GKGCMC 1 cut(s) 161
BstV1I GCAGC 1 cut(s) 160
BsuRI GGCC 1 cut(s) 316
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 2 cut(s) 48, 220
Cac8I GCNNGC 3 cut(s) 140, 157, 171
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 209
CseI GACGC 3 cut(s) 16, 47, 77
CviJI RGCY 9 cut(s) 80, 169, 173, 229, 265, 279, 316, 327, 336
CviKI_1 RGCY 9 cut(s) 80, 169, 173, 229, 265, 279, 316, 327, 336
DdeI CTNAG 2 cut(s) 62, 230
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
EaeI YGGCCR 1 cut(s) 314
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 104
FaiI YATR 4 cut(s) 114, 180, 272, 303
FaqI GGGAC 1 cut(s) 80
FauI CCCGC 1 cut(s) 148
FblI GTMKAC 1 cut(s) 36
Fnu4HI GCNGC 2 cut(s) 174, 314
Fsp4HI GCNGC 2 cut(s) 174, 314
GluI GCNGC 2 cut(s) 174, 314
HaeIII GGCC 1 cut(s) 316
HapII CCGG 2 cut(s) 32, 170
HgaI GACGC 3 cut(s) 16, 47, 77
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 2 cut(s) 107, 250
HpaII CCGG 2 cut(s) 32, 170
Hpy166II GTNNAC 2 cut(s) 21, 37
Hpy188I TCNGA 2 cut(s) 55, 347
Hpy188III TCNNGA 2 cut(s) 22, 254
Hpy8I GTNNAC 2 cut(s) 21, 37
Hpy99I CGWCG 3 cut(s) 10, 41, 86
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 1 cut(s) 245
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 333
HpyF3I CTNAG 2 cut(s) 62, 230
HpySE526I ACGT 1 cut(s) 306
KroI GCCGGC 1 cut(s) 169
KroNI GCCGGC 1 cut(s) 171
Kzo9I GATC 1 cut(s) 189
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 1 cut(s) 135
LpnPI CCDG 6 cut(s) 29, 45, 49, 183, 226, 239
Lsp1109I GCAGC 1 cut(s) 160
MaeII ACGT 1 cut(s) 306
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MboII GAAGA 1 cut(s) 176
MhlI GDGCHC 1 cut(s) 161
MluCI AATT 2 cut(s) 185, 296
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 78
MroNI GCCGGC 1 cut(s) 169
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 2 cut(s) 219, 330
MspA1I CMGCKG 1 cut(s) 313
MspI CCGG 2 cut(s) 32, 170
MwoI GCNNNNNNNGC 2 cut(s) 77, 333
NaeI GCCGGC 1 cut(s) 171
NdeII GATC 1 cut(s) 189
NgoMIV GCCGGC 1 cut(s) 169
NlaIV GGNNCC 1 cut(s) 211
PciSI GCTCTTC 1 cut(s) 159
PdiI GCCGGC 1 cut(s) 171
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 1 cut(s) 250
PflMI CCANNNNNTGG 1 cut(s) 323
PkrI GCNGC 2 cut(s) 175, 315
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 1 cut(s) 209
SalI GTCGAC 1 cut(s) 35
SapI GCTCTTC 1 cut(s) 159
SaqAI TTAA 2 cut(s) 219, 330
SatI GCNGC 2 cut(s) 174, 314
Sau3AI GATC 1 cut(s) 189
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 1 cut(s) 116
SduI GDGCHC 1 cut(s) 161
SetI ASST 5 cut(s) 18, 231, 267, 281, 309
SinI GGWCC 1 cut(s) 209
Sse9I AATT 2 cut(s) 185, 296
SsiI CCGC 2 cut(s) 155, 313
TaiI ACGT 1 cut(s) 309
TaqI TCGA 1 cut(s) 36
TasI AATT 2 cut(s) 185, 296
TauI GCSGC 1 cut(s) 316
TfiI GAWTC 1 cut(s) 250
Tru1I TTAA 2 cut(s) 219, 330
Tru9I TTAA 2 cut(s) 219, 330
TscAI CASTG 2 cut(s) 48, 220
TseI GCWGC 1 cut(s) 173
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 2 cut(s) 48, 220
Van91I CCANNNNNTGG 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 209
XapI RAATTY 1 cut(s) 296
XmiI GTMKAC 1 cut(s) 36
XmnI GAANNNNTTC 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.