AT5G58100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015985
Physical Location & Seq
Forward (+)
23507337 .. 23515074
7738 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G58100.1

Sequence Viewer

Length: 2838 bp
ATGCGGAGATTCGGGGCTGGGAACAGATCCGTTTCCAAGCTCGTACTCACCATTTGTGTAGCTATCCTGTTCATACCATCTCTATCGTATGGAGCTTCACAAGGGAACCGGAAGACGGCGAAGTCTTCTGTGTTTTCCTTGTTCAATCTTAGGGATAAAAGCAGATTTTGGAGCGAGTCTGTTTTTCGTACTGATTTTGATGATTTGGAGTCTTCCGTTCACTCGAATTCTGGTGTGCTGAATTACACTAAGTCAGGAAATATTGCGAGTTACTTAGAGCTTATGGAAGTCGATTCTGTCTATCTTCCAGTGCCAGTTAATTTTATCTTCATAGGGTTTGAAGGAAAAGGGAACCAAGACTTCAAACTCCGTCCCGAGGAACTTGAGCGTTGGTTTAATAAACTTGATCACATGTTTGAACATACACGAGTTCCACAAATCAAAGAAGTTCTCAATCCCTTTTACAAGATTAACATTGAAAAGGAGGTGCAGCACCATCTTCCTATCATCAGTCGCGTGAACTACAACTTTTCTGTTCATGCTATACAAATGGGAGAAAAGGTGACATCAGTAATTGAACACGCAATCAAAGTTTTAGCTCGCAAAGATGATGTGGCTACCAATAAAGATGAGGAAAGTGCTCTACTGCAAGTGGATGCTGAGATGATGGAATTCATTTTCACTAGCCTTGTCGAATATTTTCATCTCGAGGATGCATACAACCTTTTTATTTTGAATCCCAAGCACGATAACAAAAAAGCCAAATATGGATACCGGAGAGGGTTTTCAGAGTCAGAGATATCTTATCTGAAAGAGAACAAGGAAATACTAAAAAATCTTCTTCAGTCTGGAAAACCGTCAGAAAATATTTTAGCATTTGATATGGTGAGGAAGCCATTGTACGACAGACATCCAATGCTGAAATTTTCCTGGACAAATGCAGAGGAGACTGATACGGCTGAGTGGTTTAATGCATGCCAGGATGCTCTTAACAAGTTGGAACAGCTATCTCTTGGTAAAGATGCAGCCGAACTCATTCAGAGTAAGGTTTTGCAGTTGCTTAGAGGAAAAAATGAAGATATGAAGGTGTTCCTAGAGAAGGATTTAAGAGCTGGAGACTTTAGCAATCTTAACGCAGAATGTCTGACTGATATCTGGATCGGAAAGGGCAGGTGGGCATTTATCGATTTAACTGCTGGCCCTTTTTCATGGGGACCTTCTGTCGGTGGGGAAGGAGTGCGAACCGAACTTAGTTTGCCAAATGTGGGAACGACTATTGGTGCAGTTGCAGAAATTTCTGAAGATGAAGCAGAAGACAAACTGCAAACTGCAATTCAGGATAAATTTTCTGTATTTGGAGAGAATGACCATCAGGCTGTTGACATTCTTCTAGCGGAGATTGATGTATATGAACTATTTGCTTTCAAGCACTGCAAGGGAAGGAAGGTGAAGCTTGCTCTTTGTGAAGAACTTGATGAAAGAATGCGGGATTTAAAGACCGAGCTCCAGTCATTTGACGGAGAGGAATATGATGAGACTCATAAGAGAAAAGCCATGGATGCACTAAGACGAATGGAGAGCTGGAACTTGTTTAGTGATGAACGTGAGGAGTTCCAAAATTACACAGTTGCACGCGATACTTTCCTTGCACATTTAGGTGCTACTCTCTGGGGATCCATGAGACATATAATATCCCCATCAGTTGCTGATGGTGCATTTCATCATTATGAGAAGATCTCTTTTCAGCTTGTCTTCATCACCCAAGAGAAAGTTCGACAAATCAAACAGCTACCGGTGGATCTCAAGGCCCTCATGGATGGGCTTTCTTCGCTGTTGTTACCTTCTCAGAAGCCTTTGTTCAGCCAGCATATGTTAACACTCTCTGAGGATCCTGCATTGGCTATGGCTTTTTCAGTGGCACGAAGGGCAGCAGCTGTCCCTCTATTACTTGTCAATGGAACTTACCGGAAGACTGTTCGTTCATATCTTGATTCATCAATCCTCCAGTACCAATTACAGAGGGTGAACGATCACACTTCCCTTAAAGGAGGTCATGCCCACAGCAGGTCCACTCTTGAAATTCCTATCTTTTGGCTCATCAGTGGTGATCCGCTACTGATTGACAAGCATTATCAAGCAAAGGCGCTTTCGAACATGGTTGTTGTTGTTCAGTCGGAGGCCTCTTCATGGGAGAGCCATTTGCAATGCAACGGGCGATCACTATTATGGGATCTGAGGAGCCCCGTGAAAGCAGCCATGGCTTCTGTTGCTGAACATCTAGCTGGATTGCTTCCTCTTCATCTTGTCTACAGTGTAGCTCATGAAAGTGCAATTGAGGACTGGACATGGTCAGTAGGTTGCAATCCCTTTTCCGTCACTTCTCAAGGTTGGCTCCTTTCACAGTTTCAATCTGACACAATTGCCCGGAGCTATATGATTACAGCGCTTGAAGAATCAATACAAGCTGTCAATTCTGGGATTCATCTTTTGCGGCTGGAGAGAACGAATAAAAAGACGTTTAAGCTCTTCCAATCAAGGGAACGTGAGCTGATGAACAAGTACAAGTACGTGGTTAGCCTGTGGAGACGACTTTCGAATGTTGCTGGAGAAACACGCTATGGGGATGCCATGAGATTTCTGCATACCTTAGAGGAAGCTACTAGCTCATTTGTAAGAGAAGTGAATGCAACAGTGGGTGTTTTGCACCCAATTCACTGCACAAAAGAGAGGAAGGTGAAAGTGGAAGTGGACATGACAACAATACCTGCCTTTATCATAGTACTGATTTTACTTTACGCTGTCTTAAGGCCAAGAGCTCCTAAGCCAAAGATCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

945

Amino Acids

107.67

Weight (kDa)

6.34

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7906 PF25483 372 - 420 2.8e-10 Domain of unknown function (DUF7906)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58100 AT5G58100
fragaria_vesca FvH4_4g03800
malus_domestica MD04G1001000.v1.1
prunus_persica Prupe.1G001000_v2.0.a1
pyrus_communis pycom04g00160
rosa_chinensis RchiOBHm_Chr4g0393651
rosa_laevigata RLG00000009738
rosa_multiflora Rmu_sc0010124.1_g000004
rosa_roxburghii Rroxscaffold_5G00338820
rosa_rugosa Rorug03G0371900.1
rosa_samantha Rh4AG048400 Rh4BG044800 Rh4CG051800
rosa_wichuraiana Rw4G003840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1161
AasI GACNNNNNNGTC 1 cut(s) 121
Acc36I ACCTGC 3 cut(s) 1161, 2055, 2773
AccI GTMKAC 1 cut(s) 2307
AccII CGCG 2 cut(s) 516, 1635
AciI CCGC 5 cut(s) 4, 1394, 1486, 2111, 2491
AclWI GGATC 9 cut(s) 21, 1166, 1668, 1681, 1806, 1883, 1896, 2102, 2238
AcsI RAATTY 6 cut(s) 226, 671, 923, 1293, 1343, 2079
AcuI CTGAAG 2 cut(s) 827, 1320
AfaI GTAC 7 cut(s) 45, 190, 902, 2009, 2561, 2567, 2781
AfeI AGCGCT 1 cut(s) 2445
AfiI CCNNNNNNNGG 8 cut(s) 115, 376, 1099, 1223, 1265, 2064, 2187, 2536
AflII CTTAAG 1 cut(s) 2803
AflIII ACRYGT 1 cut(s) 411
AgeI ACCGGT 1 cut(s) 1792
AhdI GACNNNNNGTC 1 cut(s) 1220
AjnI CCWGG 2 cut(s) 929, 978
AjuI GAANNNNNNNTTGG 2 cut(s) 1755, 1787
Alw21I GWGCWC 3 cut(s) 643, 1506, 2818
Alw26I GTCTC 5 cut(s) 941, 1110, 1529, 1675, 2578
AlwI GGATC 9 cut(s) 21, 1166, 1668, 1681, 1806, 1883, 1896, 2102, 2238
AlwNI CAGNNNCTG 2 cut(s) 1706, 1934
Ama87I CYCGRG 2 cut(s) 374, 707
Aor51HI AGCGCT 1 cut(s) 2445
AoxI GGCC 4 cut(s) 1198, 1806, 2178, 2807
ApeKI GCWGC 5 cut(s) 490, 1025, 1928, 1931, 2252
ApoI RAATTY 6 cut(s) 226, 671, 923, 1293, 1343, 2079
AsiGI ACCGGT 1 cut(s) 1792
Asp700I GAANNNNTTC 3 cut(s) 699, 1035, 1088
AspLEI GCGC 2 cut(s) 2146, 2446
AspS9I GGNCC 4 cut(s) 1199, 1214, 1807, 2067
AsuC2I CCSGG 1 cut(s) 2425
AsuHPI GGTGA 8 cut(s) 40, 574, 898, 1459, 1750, 2035, 2117, 2746
AsuII TTCGAA 2 cut(s) 2150, 2594
AvaI CYCGRG 2 cut(s) 374, 707
AvaII GGWCC 2 cut(s) 1214, 2067
BamHI GGATCC 2 cut(s) 1673, 1888
BanII GRGCYC 3 cut(s) 1506, 2243, 2818
BarI GAAGNNNNNNTAC 4 cut(s) 884, 916, 2442, 2474
BauI CACGAG 1 cut(s) 426
BbsI GAAGAC 6 cut(s) 117, 119, 204, 1320, 1744, 1976
Bbv12I GWGCWC 3 cut(s) 643, 1506, 2818
BbvI GCAGC 5 cut(s) 502, 1037, 1940, 1943, 2264
BccI CCATC 7 cut(s) 85, 504, 661, 1377, 1703, 1705, 1811
BceAI ACGGC 2 cut(s) 132, 972
BcgI CGANNNNNNTGC 2 cut(s) 1175, 1209
BciT130I CCWGG 2 cut(s) 931, 980
BciVI GTATCC 1 cut(s) 764
BclI TGATCA 1 cut(s) 406
BcnI CCSGG 1 cut(s) 2425
BcoDI GTCTC 5 cut(s) 941, 1110, 1529, 1675, 2578
BfaI CTAG 5 cut(s) 684, 1094, 1391, 2279, 2661
BfmI CTRYAG 1 cut(s) 2308
BfoI RGCGCY 2 cut(s) 2147, 2447
BfrI CTTAAG 1 cut(s) 2803
BfuAI ACCTGC 3 cut(s) 1161, 2055, 2773
BfuI GTATCC 1 cut(s) 764
BglII AGATCT 1 cut(s) 1734
BisI GCNGC 6 cut(s) 491, 1026, 1929, 1932, 2253, 2492
BlsI GCNGC 6 cut(s) 492, 1027, 1930, 1933, 2254, 2493
BmcAI AGTACT 1 cut(s) 2781
Bme1390I CCNGG 3 cut(s) 931, 980, 2425
Bme18I GGWCC 2 cut(s) 1214, 2067
BmeRI GACNNNNNGTC 1 cut(s) 1220
BmeT110I CYCGRG 2 cut(s) 374, 707
BmgT120I GGNCC 4 cut(s) 1199, 1214, 1807, 2067
BmiI GGNNCC 7 cut(s) 107, 353, 1215, 1675, 1890, 2240, 2393
BmrFI CCNGG 3 cut(s) 931, 980, 2425
BmsI GCATC 6 cut(s) 646, 703, 973, 1012, 1549, 2614
BpiI GAAGAC 6 cut(s) 117, 119, 204, 1320, 1744, 1976
BplI GAGNNNNNCTC 2 cut(s) 1721, 1753
BpmI CTGGAG 5 cut(s) 1134, 1490, 1988, 2516, 2625
Bpu10I CCTNAGC 1 cut(s) 2820
Bpu14I TTCGAA 2 cut(s) 2150, 2594
BpuEI CTTGAG 3 cut(s) 404, 1787, 2367
BpuMI CCSGG 1 cut(s) 2425
Bsa29I ATCGAT 1 cut(s) 1185
BsaAI YACGTR 1 cut(s) 2569
BsaJI CCNNGG 3 cut(s) 375, 1554, 2256
BsaWI WCCGGW 4 cut(s) 108, 774, 1792, 1965
BsaXI ACNNNNNCTCC 2 cut(s) 546, 576
Bsc4I CCNNNNNNNGG 8 cut(s) 115, 376, 1099, 1223, 1265, 2064, 2187, 2536
Bse118I RCCGGY 1 cut(s) 1792
Bse1I ACTGG 5 cut(s) 308, 314, 1507, 2005, 2345
Bse3DI GCAATG 1 cut(s) 2210
BseBI CCWGG 2 cut(s) 931, 980
BseCI ATCGAT 1 cut(s) 1185
BseDI CCNNGG 3 cut(s) 375, 1554, 2256
BseGI GGATG 7 cut(s) 661, 718, 910, 988, 1564, 1822, 2629
BseLI CCNNNNNNNGG 8 cut(s) 115, 376, 1099, 1223, 1265, 2064, 2187, 2536
BseMI GCAATG 1 cut(s) 2210
BseMII CTCAG 5 cut(s) 651, 951, 1859, 1875, 2225
BseNI ACTGG 5 cut(s) 308, 314, 1507, 2005, 2345
BseRI GAGGAG 3 cut(s) 959, 1622, 2251
BseXI GCAGC 5 cut(s) 502, 1037, 1940, 1943, 2264
BseYI CCCAGC 1 cut(s) 17
BsgI GTGCAG 3 cut(s) 509, 1302, 2701
Bsh1236I CGCG 2 cut(s) 516, 1635
BshFI GGCC 4 cut(s) 1200, 1808, 2180, 2809
BshTI ACCGGT 1 cut(s) 1792
BshVI ATCGAT 1 cut(s) 1185
BsiHKAI GWGCWC 3 cut(s) 643, 1506, 2818
BsiHKCI CYCGRG 2 cut(s) 374, 707
BsiSI CCGG 5 cut(s) 109, 775, 1793, 1966, 2425
BslFI GGGAC 3 cut(s) 357, 1227, 1922
BslI CCNNNNNNNGG 8 cut(s) 115, 376, 1099, 1223, 1265, 2064, 2187, 2536
BsmAI GTCTC 5 cut(s) 941, 1110, 1529, 1675, 2578
BsmBI CGTCTC 1 cut(s) 2578
BsmFI GGGAC 3 cut(s) 357, 1227, 1922
BsmI GAATGC 2 cut(s) 1488, 2689
BsnI GGCC 4 cut(s) 1200, 1808, 2180, 2809
BsoBI CYCGRG 2 cut(s) 374, 707
Bsp119I TTCGAA 2 cut(s) 2150, 2594
Bsp1286I GDGCHC 4 cut(s) 643, 1506, 2243, 2818
Bsp19I CCATGG 2 cut(s) 1554, 2256
BspACI CCGC 5 cut(s) 4, 1394, 1486, 2111, 2491
BspANI GGCC 4 cut(s) 1200, 1808, 2180, 2809
BspCNI CTCAG 5 cut(s) 652, 952, 1858, 1876, 2226
BspDI ATCGAT 1 cut(s) 1185
BspFNI CGCG 2 cut(s) 516, 1635
BspHI TCATGA 1 cut(s) 2320
BspLI GGNNCC 7 cut(s) 107, 353, 1215, 1675, 1890, 2240, 2393
BspMI ACCTGC 3 cut(s) 1161, 2055, 2773
BspPI GGATC 9 cut(s) 21, 1166, 1668, 1681, 1806, 1883, 1896, 2102, 2238
BspQI GCTCTTC 1 cut(s) 2531
BspT104I TTCGAA 2 cut(s) 2150, 2594
BspTI CTTAAG 1 cut(s) 2803
BsrDI GCAATG 1 cut(s) 2210
BsrFI RCCGGY 1 cut(s) 1792
BsrI ACTGG 5 cut(s) 308, 314, 1507, 2005, 2345
BssAI RCCGGY 1 cut(s) 1792
BssECI CCNNGG 3 cut(s) 375, 1554, 2256
BssSI CACGAG 1 cut(s) 426
BssT1I CCWWGG 2 cut(s) 1554, 2256
Bst2BI CACGAG 1 cut(s) 426
Bst2UI CCWGG 2 cut(s) 931, 980
Bst4CI ACNGT 6 cut(s) 858, 1627, 1975, 2312, 2403, 2692
Bst6I CTCTTC 3 cut(s) 2188, 2301, 2531
BstAFI CTTAAG 1 cut(s) 2803
BstBAI YACGTR 1 cut(s) 2569
BstBI TTCGAA 2 cut(s) 2150, 2594
BstC8I GCNNGC 6 cut(s) 601, 976, 1198, 1455, 1633, 1865
BstDSI CCRYGG 2 cut(s) 1554, 2256
BstENI CCTNNNNNAGG 1 cut(s) 1097
BstF5I GGATG 7 cut(s) 661, 718, 910, 988, 1564, 1822, 2629
BstFNI CGCG 2 cut(s) 516, 1635
BstH2I RGCGCY 2 cut(s) 2147, 2447
BstHHI GCGC 2 cut(s) 2146, 2446
BstMAI GTCTC 5 cut(s) 941, 1110, 1529, 1675, 2578
BstMWI GCNNNNNNNGC 6 cut(s) 1559, 1712, 1828, 1925, 2258, 2267
BstNI CCWGG 2 cut(s) 931, 980
BstNSI RCATGY 2 cut(s) 415, 978
BstSCI CCNGG 3 cut(s) 929, 978, 2423
BstSFI CTRYAG 1 cut(s) 2308
BstUI CGCG 2 cut(s) 516, 1635
BstV1I GCAGC 5 cut(s) 502, 1037, 1940, 1943, 2264
BstV2I GAAGAC 6 cut(s) 117, 119, 204, 1320, 1744, 1976
BstX2I RGATCY 6 cut(s) 26, 1673, 1734, 1798, 1888, 2230
BstYI RGATCY 6 cut(s) 26, 1673, 1734, 1798, 1888, 2230
Bsu15I ATCGAT 1 cut(s) 1185
BsuI GTATCC 1 cut(s) 764
BsuRI GGCC 4 cut(s) 1200, 1808, 2180, 2809
BsuTUI ATCGAT 1 cut(s) 1185
BtgI CCRYGG 2 cut(s) 1554, 2256
BtsCI GGATG 7 cut(s) 661, 718, 910, 988, 1564, 1822, 2629
BtsI GCAGTG 2 cut(s) 1429, 2713
BtsIMutI CAGTG 7 cut(s) 315, 1429, 1920, 2107, 2317, 2697, 2713
BveI ACCTGC 3 cut(s) 1161, 2055, 2773
Cac8I GCNNGC 6 cut(s) 601, 976, 1198, 1455, 1633, 1865
CaiI CAGNNNCTG 2 cut(s) 1706, 1934
CciI TCATGA 1 cut(s) 2320
CfoI GCGC 2 cut(s) 2146, 2446
Cfr10I RCCGGY 1 cut(s) 1792
Cfr13I GGNCC 4 cut(s) 1199, 1214, 1807, 2067
ClaI ATCGAT 1 cut(s) 1185
Csp6I GTAC 7 cut(s) 44, 189, 901, 2008, 2560, 2566, 2780
CspAI ACCGGT 1 cut(s) 1792
CviQI GTAC 7 cut(s) 44, 189, 901, 2008, 2560, 2566, 2780
DraI TTTAAA 1 cut(s) 1494
DrdI GACNNNNNNGTC 1 cut(s) 121
DriI GACNNNNNGTC 1 cut(s) 1220
DseDI GACNNNNNNGTC 1 cut(s) 121
Eam1104I CTCTTC 3 cut(s) 2188, 2301, 2531
Eam1105I GACNNNNNGTC 1 cut(s) 1220
EarI CTCTTC 3 cut(s) 2188, 2301, 2531
Ecl136II GAGCTC 2 cut(s) 1504, 2816
Eco130I CCWWGG 2 cut(s) 1554, 2256
Eco147I AGGCCT 1 cut(s) 2180
Eco24I GRGCYC 3 cut(s) 1506, 2243, 2818
Eco32I GATATC 2 cut(s) 801, 1153
Eco47I GGWCC 2 cut(s) 1214, 2067
Eco47III AGCGCT 1 cut(s) 2445
Eco53kI GAGCTC 2 cut(s) 1504, 2816
Eco57I CTGAAG 2 cut(s) 827, 1320
Eco88I CYCGRG 2 cut(s) 374, 707
EcoICRI GAGCTC 2 cut(s) 1504, 2816
EcoNI CCTNNNNNAGG 1 cut(s) 1097
EcoO109I RGGNCCY 2 cut(s) 1214, 1807
EcoRI GAATTC 2 cut(s) 226, 671
EcoRII CCWGG 2 cut(s) 929, 978
EcoRV GATATC 2 cut(s) 801, 1153
EcoT14I CCWWGG 2 cut(s) 1554, 2256
EcoT22I ATGCAT 2 cut(s) 718, 976
EcoT38I GRGCYC 3 cut(s) 1506, 2243, 2818
ErhI CCWWGG 2 cut(s) 1554, 2256
Esp3I CGTCTC 1 cut(s) 2578
FaqI GGGAC 3 cut(s) 357, 1227, 1922
FauI CCCGC 1 cut(s) 1479
FauNDI CATATG 1 cut(s) 1869
FbaI TGATCA 1 cut(s) 406
FblI GTMKAC 1 cut(s) 2307
Fnu4HI GCNGC 6 cut(s) 491, 1026, 1929, 1932, 2253, 2492
FokI GGATG 7 cut(s) 668, 725, 897, 995, 1571, 1829, 2636
FriOI GRGCYC 3 cut(s) 1506, 2243, 2818
Fsp4HI GCNGC 6 cut(s) 491, 1026, 1929, 1932, 2253, 2492
FspBI CTAG 5 cut(s) 684, 1094, 1391, 2279, 2661
GlaI GCGC 2 cut(s) 2145, 2445
GluI GCNGC 6 cut(s) 491, 1026, 1929, 1932, 2253, 2492
GsaI CCCAGC 1 cut(s) 21
GsuI CTGGAG 5 cut(s) 1134, 1490, 1988, 2516, 2625
HaeII RGCGCY 2 cut(s) 2147, 2447
HaeIII GGCC 4 cut(s) 1200, 1808, 2180, 2809
HapII CCGG 5 cut(s) 109, 775, 1793, 1966, 2425
HhaI GCGC 2 cut(s) 2146, 2446
Hin6I GCGC 2 cut(s) 2144, 2444
HinP1I GCGC 2 cut(s) 2144, 2444
HincII GTYRAC 2 cut(s) 1381, 1875
HindII GTYRAC 2 cut(s) 1381, 1875
HindIII AAGCTT 1 cut(s) 1451
HpaI GTTAAC 1 cut(s) 1875
HpaII CCGG 5 cut(s) 109, 775, 1793, 1966, 2425
HphI GGTGA 8 cut(s) 40, 574, 898, 1459, 1750, 2035, 2117, 2746
Hpy166II GTNNAC 8 cut(s) 220, 520, 1381, 1875, 2026, 2070, 2308, 2749
Hpy188III TCNNGA 9 cut(s) 255, 374, 707, 849, 1156, 1337, 1988, 2075, 2321
Hpy8I GTNNAC 8 cut(s) 220, 520, 1381, 1875, 2026, 2070, 2308, 2749
HpyCH4III ACNGT 6 cut(s) 858, 1627, 1975, 2312, 2403, 2692
HpyCH4IV ACGT 4 cut(s) 1603, 2516, 2542, 2568
HpyF10VI GCNNNNNNNGC 6 cut(s) 1559, 1712, 1828, 1925, 2258, 2267
HpySE526I ACGT 4 cut(s) 1603, 2516, 2542, 2568
HspAI GCGC 2 cut(s) 2144, 2444
Ksp22I TGATCA 1 cut(s) 406
KspAI GTTAAC 1 cut(s) 1875
LguI GCTCTTC 1 cut(s) 2531
LmnI GCTCC 7 cut(s) 92, 171, 1509, 2238, 2397, 2427, 2821
Lsp1109I GCAGC 5 cut(s) 502, 1037, 1940, 1943, 2264
LweI GCATC 6 cut(s) 646, 703, 973, 1012, 1549, 2614
MaeI CTAG 5 cut(s) 684, 1094, 1391, 2279, 2661
MaeII ACGT 4 cut(s) 1603, 2516, 2542, 2568
MaeIII GTNAC 4 cut(s) 269, 562, 1836, 2374
MfeI CAATTG 2 cut(s) 2331, 2418
MflI RGATCY 6 cut(s) 26, 1673, 1734, 1798, 1888, 2230
MhlI GDGCHC 4 cut(s) 643, 1506, 2243, 2818
MlyI GAGTC 4 cut(s) 185, 218, 800, 1531
MmeI TCCRAC 2 cut(s) 978, 2154
Mph1103I ATGCAT 2 cut(s) 718, 976
MroXI GAANNNNTTC 3 cut(s) 699, 1035, 1088
MslI CAYNNNNRTG 4 cut(s) 1656, 1725, 2224, 2325
MspA1I CMGCKG 1 cut(s) 1934
MspCI CTTAAG 1 cut(s) 2803
MspI CCGG 5 cut(s) 109, 775, 1793, 1966, 2425
MspR9I CCNGG 3 cut(s) 931, 980, 2425
MunI CAATTG 2 cut(s) 2331, 2418
Mva1269I GAATGC 2 cut(s) 1488, 2689
MvaI CCWGG 2 cut(s) 931, 980
MvnI CGCG 2 cut(s) 516, 1635
MwoI GCNNNNNNNGC 6 cut(s) 1559, 1712, 1828, 1925, 2258, 2267
NciI CCSGG 1 cut(s) 2425
NcoI CCATGG 2 cut(s) 1554, 2256
NdeI CATATG 1 cut(s) 1869
NlaIV GGNNCC 7 cut(s) 107, 353, 1215, 1675, 1890, 2240, 2393
NmuCI GTSAC 2 cut(s) 562, 2374
NsiI ATGCAT 2 cut(s) 718, 976
NspI RCATGY 2 cut(s) 415, 978
NspV TTCGAA 2 cut(s) 2150, 2594
PaeI GCATGC 1 cut(s) 978
PaeR7I CTCGAG 1 cut(s) 707
PagI TCATGA 1 cut(s) 2320
PaqCI CACCTGC 1 cut(s) 1161
PceI AGGCCT 1 cut(s) 2180
PciI ACATGT 1 cut(s) 411
PciSI GCTCTTC 1 cut(s) 2531
PctI GAATGC 2 cut(s) 1488, 2689
PdmI GAANNNNTTC 3 cut(s) 699, 1035, 1088
PfeI GAWTC 6 cut(s) 9, 293, 736, 1991, 2453, 2479
PflFI GACNNNGTC 1 cut(s) 2347
PfoI TCCNGGA 1 cut(s) 929
PinAI ACCGGT 1 cut(s) 1792
PkrI GCNGC 6 cut(s) 492, 1027, 1930, 1933, 2254, 2493
PleI GAGTC 4 cut(s) 184, 217, 799, 1531
PpsI GAGTC 4 cut(s) 184, 217, 799, 1531
Ppu21I YACGTR 1 cut(s) 2569
PpuMI RGGWCCY 1 cut(s) 1214
PscI ACATGT 1 cut(s) 411
Psp124BI GAGCTC 2 cut(s) 1506, 2818
Psp5II RGGWCCY 1 cut(s) 1214
Psp6I CCWGG 2 cut(s) 929, 978
PspFI CCCAGC 1 cut(s) 17
PspGI CCWGG 2 cut(s) 929, 978
PspN4I GGNNCC 7 cut(s) 107, 353, 1215, 1675, 1890, 2240, 2393
PspPI GGNCC 4 cut(s) 1199, 1214, 1807, 2067
PspPPI RGGWCCY 1 cut(s) 1214
PstNI CAGNNNCTG 2 cut(s) 1706, 1934
PsuI RGATCY 6 cut(s) 26, 1673, 1734, 1798, 1888, 2230
PsyI GACNNNGTC 1 cut(s) 2347
PvuII CAGCTG 1 cut(s) 1934
RsaI GTAC 7 cut(s) 45, 190, 902, 2009, 2561, 2567, 2781
RsaNI GTAC 7 cut(s) 44, 189, 901, 2008, 2560, 2566, 2780
RseI CAYNNNNRTG 4 cut(s) 1656, 1725, 2224, 2325
SacI GAGCTC 2 cut(s) 1506, 2818
SapI GCTCTTC 1 cut(s) 2531
SatI GCNGC 6 cut(s) 491, 1026, 1929, 1932, 2253, 2492
Sau96I GGNCC 4 cut(s) 1199, 1214, 1807, 2067
ScaI AGTACT 1 cut(s) 2781
SchI GAGTC 4 cut(s) 185, 218, 800, 1531
ScrFI CCNGG 3 cut(s) 931, 980, 2425
SduI GDGCHC 4 cut(s) 643, 1506, 2243, 2818
SfaNI GCATC 6 cut(s) 646, 703, 973, 1012, 1549, 2614
SfcI CTRYAG 1 cut(s) 2308
Sfr274I CTCGAG 1 cut(s) 707
SfuI TTCGAA 2 cut(s) 2150, 2594
SinI GGWCC 2 cut(s) 1214, 2067
SlaI CTCGAG 1 cut(s) 707
SmiMI CAYNNNNRTG 4 cut(s) 1656, 1725, 2224, 2325
SmlI CTYRAG 5 cut(s) 383, 707, 1802, 2382, 2803
SmoI CTYRAG 5 cut(s) 383, 707, 1802, 2382, 2803
SphI GCATGC 1 cut(s) 978
SseBI AGGCCT 1 cut(s) 2180
SsiI CCGC 5 cut(s) 4, 1394, 1486, 2111, 2491
SspI AATATT 3 cut(s) 262, 698, 868
SspMI CTAG 5 cut(s) 684, 1094, 1391, 2279, 2661
SstI GAGCTC 2 cut(s) 1506, 2818
StuI AGGCCT 1 cut(s) 2180
StyD4I CCNGG 3 cut(s) 929, 978, 2423
StyI CCWWGG 2 cut(s) 1554, 2256
TaaI ACNGT 6 cut(s) 858, 1627, 1975, 2312, 2403, 2692
TaiI ACGT 4 cut(s) 1606, 2519, 2545, 2571
TaqI TCGA 8 cut(s) 224, 291, 693, 708, 1185, 1774, 2150, 2594
TaqII GACCGA 1 cut(s) 1514
TatI WGTACW 2 cut(s) 2559, 2779
TauI GCSGC 1 cut(s) 2494
TfiI GAWTC 6 cut(s) 9, 293, 736, 1991, 2453, 2479
TscAI CASTG 7 cut(s) 315, 1436, 1920, 2107, 2317, 2697, 2720
TseFI GTSAC 2 cut(s) 562, 2374
TseI GCWGC 5 cut(s) 490, 1025, 1928, 1931, 2252
Tsp45I GTSAC 2 cut(s) 562, 2374
TspGWI ACGGA 5 cut(s) 19, 205, 359, 1533, 2362
TspRI CASTG 7 cut(s) 315, 1436, 1920, 2107, 2317, 2697, 2720
Tth111I GACNNNGTC 1 cut(s) 2347
Vha464I CTTAAG 1 cut(s) 2803
VpaK11BI GGWCC 2 cut(s) 1214, 2067
XagI CCTNNNNNAGG 1 cut(s) 1097
XapI RAATTY 6 cut(s) 226, 671, 923, 1293, 1343, 2079
XceI RCATGY 2 cut(s) 415, 978
XhoI CTCGAG 1 cut(s) 707
XmiI GTMKAC 1 cut(s) 2307
XmnI GAANNNNTTC 3 cut(s) 699, 1035, 1088
XspI CTAG 5 cut(s) 684, 1094, 1391, 2279, 2661
ZrmI AGTACT 1 cut(s) 2781
Zsp2I ATGCAT 2 cut(s) 718, 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.