Rh4CG051800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0015985
Physical Location & Seq
Reverse (-)
10263935 .. 10267633
3699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG051800.1

Sequence Viewer

Length: 2859 bp
ATGGATCGCCGTCCCCTTCCTCGCCGTCGCCGGCCGTGCTTCATCGCCGCCGTCATCATTTCCAGTCTTCTACTCGTCTTCCTACCTCAATCGGCGTCCGGATCTCCCTCCAGAACTCGCAGACCCACCACCAAGCCCTCATCGGTCTTCTCTCTCTTCAACCTCAAGCAGAAGTCCCGCTTCTGGAGCGAGTCCGTCATCCGCAACGACTTCGCCGACCTGGAGTCTCCGGCCGCCGCGAAAACCACCTTCGCCAACTTCACCGCCGCAGGTAACGTCGCAAATTACCTAAAACTCCTTCCGATTGAGTCTATGTACCTGCCGGTCCCCGTGAATTTCATCTTCGTTGGATTCGACGGGAACGGGAACCAGGAGTTCAAGCTTCACGCTGAGGAGCTTGAGCGGTGGTTCACGAAAATTGACCATGTATTTGCGCACGCTAGGGTTCCCCAAATTGGGGAAACCCTAACCCCGTTTTACAAGATTAGTGTAGATAAGGAGCAGAGGCATCATCTTCCTCTGGTGAGTCACGTGAAATACAATCTTTCTGTTCATGCGATTCAAATGGGGGAGAAGGTGACCTCGATTTTCGAGAAGGCTGTGAGTGTTTTGGCTCGAAAAGATGAGGTGAGTGGTGATGGAGATGTTGCTTTGTGGCAAGTAGATGTGGATATGATGGATGTGCTTTTCAGTAGCCTTGTTGGGTATCTTGAGATTGGCAATGCATATAACATTTTCGTGTTGAATCCCAAGCGAGACTCGAAGAGGGTTAAGTATGGTTACCGGAGAGGGCTGTCGGATTCGGAAATGAGGTATCTGAAGGCGAATGTGAGTGTGATGCAGAGCAAGATTGTTGAGTCGGCGGGGAGGGTTCCGGATACCGTGGCTGCGCTTGATAAGATTAAGGGTAAGAGGCCGTTGTATGAGAAGCATCCGATGGCAAAGTTTGCGTGGTCTGTGACGGAAGATACTGATACGGTGGAGTGGTACAATGCGTGTGAACAAGCGCTGGACAATGTGGAGAAGTTGTATCAAGGGAAGGAAACTGGGGATGTAATTCAGAACAAAGTCGTACAGTTATTGAATGGGAAGCATGAGGATATGAAGCTTCTTTACAATAAGGGATTGAAATCTGGAGACTTTAGTGATCTTCATGCTGAATGTCTTACAGATACATGGATTGGAAAGGAGAGGTGGGCATTTATTGACTTGAGTGCAGGCCCTTTCTCATGGGGACCAGCTGTTGGTGGGGAAGGTGTGCGGACAGAACTAAGTATTCCCAATGTACAAAAAACAATTGGTGCAGTTTCAGAAATTACAGAGGATGAAGCTGAAGATCGCTTGCAAGATGCTATTCAGGAAAAGTTTGCAGTATTTGGTGATAAAGATCATCAGGCTATTGATATTCTTCTAGCAGAGATTGATATATATGAGCTTTTTGCTTTCAAACATTGCAAAGGAAGGAAAGTAAAGCTTGCGCTTTGTGAAGAACTTGATGAGAGAATGAGGGATTTGAAGAATGAGCTCCAATCATTTGAAGGTGAAGAATATGATGATAGTCATAAGAAAAAGGCTGTAGATGCATTAAAGCGAATGGAGAATTGGAATTTGTTTAGTGATACTCAGGAGGAATTTCAGAATTACACAGTTGCACGTGATACTTTTCTTTCGCATTTAGGTGCAACATTGTGGGGGTCTATGAGACACATTATATCACCTTCCGTAGCAGATGGTGCATTCCATCATTATGATACAATATCGTTTCAGTTGTTTTTCATCACACAGGAGAAAGTTAGACATATTAAACAATTGCCTGTTGATCTCAAGGCTCTCCAGCACGGACTCTCCTCATTGTTGTTACCATCTCAGACACCCGCTTTCAGTCAGCATATGCTACCACTTTCTGAGGATCCTGCTTTGGCAATGGCCTTTTCGGTGGCACGAAGAGCGGCAGCTGTCCCACTATTGCTTGTTAATGGAACTTACAGGAAAACTGTTCGTGCCTATCTTGATTCCTCCATTGTCCAGTATCAGTTGCAGAGACTGAATGATCAAGGCTCTTTAAAAGGGAAGCTTGCCCACAGCCGATCTACACTGGAAGTTCCAATCTTTTGGTTCATCCATGGTGAACCGTTGTTAGTTGACAAGCATTATCAGGCAAAGGCTCTTTCTGATATGGTTATTGTTGTTCAGTCAGATACTTCATCATGGGAAAGCCACTTGCAGTGTAATGGGCAGCCACTTTTCTGGGATTTGAGGAGGCCCATAAAAGCTGCATTGGCTGCTGCTTCTGAACATCTTGCTGGTTTGCTACCCCTTCATTTAGCTTACAGTCATGCCCGTGAAACTGCAATTGAGGACTGGATGTGGTCAGTTGGGTGCGACCCTCATTCCATTACTTCTCAAGGCTGGACCCTTTCACAATTTCAGTCTGATACAATTGCTCGGAGCTATATCATCACAACTCTAGACGAGTCAATACAGAGTGTCAATTCAGCCATTCATCTTCTAGGCTTGGAGCGTACAACTGAAAAAACCTTTCGACTTGTTCTGTCCGAAGAGCGGGATCTACTTAACAAGTACAACTATGTTGTCAGTCTGTGGAAAAGGATTTCCACTACTACTGGAGAATTGCGATATGCAGATGCCATGAGAATGCTACATACCCTAGAGGATGCATCAAAACAGTTTGTTGATCAAGTAAATGCAACTATAGCCAATCTCCACCCAATCCACTGTACAAAAGAGAGGAGAGTACATGTGGAGTATAATTTGTCTACAATTCCAGCTTTCTTGGTTGTCCTGGGTGTTCTCTATCTTGTGTTAAGGCCGAGACGACCAAAGCCAAAAGTTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

952

Amino Acids

107.91

Weight (kDa)

6.73

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7906 PF25483 351 - 433 4.4e-12 Domain of unknown function (DUF7906)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58100 AT5G58100
fragaria_vesca FvH4_4g03800
malus_domestica MD04G1001000.v1.1
prunus_persica Prupe.1G001000_v2.0.a1
pyrus_communis pycom04g00160
rosa_chinensis RchiOBHm_Chr4g0393651
rosa_laevigata RLG00000009738
rosa_multiflora Rmu_sc0010124.1_g000004
rosa_roxburghii Rroxscaffold_5G00338820
rosa_rugosa Rorug03G0371900.1
rosa_samantha Rh4AG048400 Rh4BG044800 Rh4CG051800
rosa_wichuraiana Rw4G003840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 435
Acc36I ACCTGC 2 cut(s) 260, 327
AccB7I CCANNNNNTGG 1 cut(s) 1244
AccBSI CCGCTC 3 cut(s) 403, 1949, 2563
AccI GTMKAC 1 cut(s) 2777
AccII CGCG 1 cut(s) 239
AccIII TCCGGA 2 cut(s) 98, 874
AclWI GGATC 5 cut(s) 12, 109, 1904, 1917, 2574
AcoI YGGCCR 2 cut(s) 32, 231
AcsI RAATTY 3 cut(s) 334, 1606, 1631
AcuI CTGAAG 2 cut(s) 839, 1353
AcvI CACGTG 2 cut(s) 532, 1655
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 8 cut(s) 317, 989, 1074, 1287, 2524, 2582, 2740, 2757
AfeI AGCGCT 1 cut(s) 1008
AfiI CCNNNNNNNGG 6 cut(s) 183, 455, 456, 457, 1244, 2562
AflIII ACRYGT 1 cut(s) 2758
AgsI TTSAA 9 cut(s) 160, 379, 563, 745, 1084, 1129, 1447, 1516, 1538
AhdI GACNNNNNGTC 1 cut(s) 223
AjnI CCWGG 3 cut(s) 219, 369, 2802
AjuI GAANNNNNNNTTGG 2 cut(s) 1585, 1617
Alw21I GWGCWC 1 cut(s) 1527
Alw26I GTCTC 6 cut(s) 231, 750, 1131, 1696, 2035, 2827
AlwI GGATC 5 cut(s) 12, 109, 1904, 1917, 2574
AlwNI CAGNNNCTG 1 cut(s) 2044
Aor13HI TCCGGA 2 cut(s) 98, 874
Aor51HI AGCGCT 1 cut(s) 1008
AoxI GGCC 7 cut(s) 32, 231, 914, 1219, 1926, 2261, 2828
ApeKI GCWGC 6 cut(s) 887, 1952, 2236, 2273, 2282, 2285
ApoI RAATTY 3 cut(s) 334, 1606, 1631
ArsI GACNNNNNNTTYG 2 cut(s) 413, 445
AspLEI GCGC 4 cut(s) 436, 892, 1009, 1480
AspS9I GGNCC 5 cut(s) 325, 1220, 1235, 2262, 2412
AsuHPI GGTGA 9 cut(s) 253, 535, 589, 640, 647, 1391, 1553, 1707, 2138
AvaII GGWCC 3 cut(s) 325, 1235, 2412
BamHI GGATCC 1 cut(s) 1909
BanII GRGCYC 1 cut(s) 1527
BarI GAAGNNNNNNTAC 2 cut(s) 1097, 1129
BbrPI CACGTG 2 cut(s) 532, 1655
BbsI GAAGAC 3 cut(s) 59, 70, 139
Bbv12I GWGCWC 1 cut(s) 1527
BbvCI CCTCAGC 1 cut(s) 390
BbvI GCAGC 6 cut(s) 874, 1964, 2248, 2260, 2269, 2272
BccI CCATC 6 cut(s) 632, 670, 931, 1724, 1749, 1870
BceAI ACGGC 4 cut(s) 9, 19, 35, 901
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BciT130I CCWGG 3 cut(s) 221, 371, 2804
BciVI GTATCC 1 cut(s) 871
BclI TGATCA 2 cut(s) 2050, 2695
BcoDI GTCTC 6 cut(s) 231, 750, 1131, 1696, 2035, 2827
BfaI CTAG 5 cut(s) 441, 1412, 2468, 2510, 2669
BfmI CTRYAG 2 cut(s) 1575, 2712
BfoI RGCGCY 1 cut(s) 1010
BfuAI ACCTGC 2 cut(s) 260, 327
BfuI GTATCC 1 cut(s) 871
Bme1390I CCNGG 3 cut(s) 221, 371, 2804
Bme18I GGWCC 3 cut(s) 325, 1235, 2412
BmeRI GACNNNNNGTC 1 cut(s) 223
BmgT120I GGNCC 5 cut(s) 325, 1220, 1235, 2262, 2412
BmiI GGNNCC 7 cut(s) 327, 368, 447, 873, 1236, 1911, 2414
BmrFI CCNGG 3 cut(s) 221, 371, 2804
BmrI ACTGGG 1 cut(s) 1056
BmsI GCATC 8 cut(s) 517, 828, 940, 1339, 1570, 2635, 2665, 2687
BmuI ACTGGG 1 cut(s) 1056
BpiI GAAGAC 3 cut(s) 59, 70, 139
BpmI CTGGAG 6 cut(s) 94, 205, 242, 1155, 1817, 2646
Bpu10I CCTNAGC 1 cut(s) 390
BpuEI CTTGAG 6 cut(s) 149, 419, 731, 1231, 1808, 2388
BsaAI YACGTR 2 cut(s) 532, 1655
BsaBI GATNNNNATC 2 cut(s) 1129, 1386
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 3 cut(s) 882, 2122, 2803
BsaWI WCCGGW 3 cut(s) 98, 783, 874
BsaXI ACNNNNNCTCC 4 cut(s) 1013, 1043, 1829, 1859
Bsc4I CCNNNNNNNGG 6 cut(s) 183, 455, 456, 457, 1244, 2562
Bse118I RCCGGY 2 cut(s) 30, 322
Bse1I ACTGG 6 cut(s) 63, 1051, 2026, 2100, 2366, 2629
Bse3DI GCAATG 3 cut(s) 727, 1450, 1929
Bse8I GATNNNNATC 2 cut(s) 1129, 1386
BseAI TCCGGA 2 cut(s) 98, 874
BseBI CCWGG 3 cut(s) 221, 371, 2804
BseDI CCNNGG 3 cut(s) 882, 2122, 2803
BseGI GGATG 8 cut(s) 198, 685, 931, 1057, 1330, 2118, 2370, 2680
BseJI GATNNNNATC 2 cut(s) 1129, 1386
BseLI CCNNNNNNNGG 6 cut(s) 183, 455, 456, 457, 1244, 2562
BseMI GCAATG 3 cut(s) 727, 1450, 1929
BseMII CTCAG 4 cut(s) 381, 1637, 1880, 1896
BseNI ACTGG 6 cut(s) 63, 1051, 2026, 2100, 2366, 2629
BseRI GAGGAG 4 cut(s) 407, 1837, 2272, 2764
BseX3I CGGCCG 2 cut(s) 32, 231
BseXI GCAGC 6 cut(s) 874, 1964, 2248, 2260, 2269, 2272
BsgI GTGCAG 2 cut(s) 1236, 1323
Bsh1236I CGCG 1 cut(s) 239
Bsh1285I CGRYCG 2 cut(s) 35, 234
BshFI GGCC 7 cut(s) 34, 233, 916, 1221, 1928, 2263, 2830
BsiEI CGRYCG 2 cut(s) 35, 234
BsiHKAI GWGCWC 1 cut(s) 1527
BsiSI CCGG 6 cut(s) 31, 99, 230, 323, 784, 875
BslFI GGGAC 4 cut(s) 160, 311, 1248, 1943
BslI CCNNNNNNNGG 6 cut(s) 183, 455, 456, 457, 1244, 2562
BsmAI GTCTC 6 cut(s) 231, 750, 1131, 1696, 2035, 2827
BsmBI CGTCTC 1 cut(s) 2827
BsmFI GGGAC 4 cut(s) 160, 311, 1248, 1943
BsmI GAATGC 2 cut(s) 1736, 2661
BsnI GGCC 7 cut(s) 34, 233, 916, 1221, 1928, 2263, 2830
Bsp1286I GDGCHC 1 cut(s) 1527
Bsp13I TCCGGA 2 cut(s) 98, 874
Bsp1407I TGTACA 2 cut(s) 1285, 2738
Bsp19I CCATGG 1 cut(s) 2122
BspANI GGCC 7 cut(s) 34, 233, 916, 1221, 1928, 2263, 2830
BspCNI CTCAG 4 cut(s) 382, 1636, 1879, 1897
BspEI TCCGGA 2 cut(s) 98, 874
BspFNI CGCG 1 cut(s) 239
BspLI GGNNCC 7 cut(s) 327, 368, 447, 873, 1236, 1911, 2414
BspMI ACCTGC 2 cut(s) 260, 327
BspPI GGATC 5 cut(s) 12, 109, 1904, 1917, 2574
BspQI GCTCTTC 2 cut(s) 1939, 2553
BsrBI CCGCTC 3 cut(s) 403, 1949, 2563
BsrDI GCAATG 3 cut(s) 727, 1450, 1929
BsrFI RCCGGY 2 cut(s) 30, 322
BsrGI TGTACA 2 cut(s) 1285, 2738
BsrI ACTGG 6 cut(s) 63, 1051, 2026, 2100, 2366, 2629
BssAI RCCGGY 2 cut(s) 30, 322
BssECI CCNNGG 3 cut(s) 882, 2122, 2803
BssNI GRCGYC 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 2122
Bst2UI CCWGG 3 cut(s) 221, 371, 2804
Bst4CI ACNGT 9 cut(s) 883, 979, 1077, 1648, 1996, 2133, 2333, 2688, 2738
Bst6I CTCTTC 4 cut(s) 161, 758, 1939, 2553
BstACI GRCGYC 1 cut(s) 95
BstAPI GCANNNNNTGC 3 cut(s) 947, 1733, 2282
BstAUI TGTACA 2 cut(s) 1285, 2738
BstBAI YACGTR 2 cut(s) 532, 1655
BstC8I GCNNGC 6 cut(s) 32, 438, 1219, 1343, 1476, 2076
BstDEI CTNAG 5 cut(s) 390, 1271, 1623, 1866, 1905
BstDSI CCRYGG 2 cut(s) 882, 2122
BstEII GGTNACC 2 cut(s) 577, 779
BstF5I GGATG 8 cut(s) 198, 685, 931, 1057, 1330, 2118, 2370, 2680
BstFNI CGCG 1 cut(s) 239
BstH2I RGCGCY 1 cut(s) 1010
BstHHI GCGC 4 cut(s) 436, 892, 1009, 1480
BstMAI GTCTC 6 cut(s) 231, 750, 1131, 1696, 2035, 2827
BstMCI CGRYCG 2 cut(s) 35, 234
BstMWI GCNNNNNNNGC 9 cut(s) 36, 186, 947, 1580, 1733, 1946, 2279, 2282, 2714
BstNI CCWGG 3 cut(s) 221, 371, 2804
BstNSI RCATGY 1 cut(s) 2762
BstPI GGTNACC 2 cut(s) 577, 779
BstSCI CCNGG 3 cut(s) 219, 369, 2802
BstSFI CTRYAG 2 cut(s) 1575, 2712
BstUI CGCG 1 cut(s) 239
BstV1I GCAGC 6 cut(s) 874, 1964, 2248, 2260, 2269, 2272
BstV2I GAAGAC 3 cut(s) 59, 70, 139
BstX2I RGATCY 3 cut(s) 101, 1909, 2566
BstXI CCANNNNNNTGG 2 cut(s) 2112, 2247
BstYI RGATCY 3 cut(s) 101, 1909, 2566
BstZI CGGCCG 2 cut(s) 32, 231
BsuI GTATCC 1 cut(s) 871
BsuRI GGCC 7 cut(s) 34, 233, 916, 1221, 1928, 2263, 2830
BtgI CCRYGG 2 cut(s) 882, 2122
BtgZI GCGATG 1 cut(s) 28
BtsCI GGATG 8 cut(s) 198, 685, 931, 1057, 1330, 2118, 2370, 2680
BtsI GCAGTG 1 cut(s) 2231
BtsIMutI CAGTG 3 cut(s) 2093, 2231, 2734
BveI ACCTGC 2 cut(s) 260, 327
Cac8I GCNNGC 6 cut(s) 32, 438, 1219, 1343, 1476, 2076
CaiI CAGNNNCTG 1 cut(s) 2044
CfoI GCGC 4 cut(s) 436, 892, 1009, 1480
Cfr10I RCCGGY 2 cut(s) 30, 322
Cfr13I GGNCC 5 cut(s) 325, 1220, 1235, 2262, 2412
CseI GACGC 1 cut(s) 84
Csp6I GTAC 8 cut(s) 316, 988, 1073, 1286, 2523, 2581, 2739, 2756
CviQI GTAC 8 cut(s) 316, 988, 1073, 1286, 2523, 2581, 2739, 2756
DdeI CTNAG 5 cut(s) 390, 1271, 1623, 1866, 1905
DraI TTTAAA 1 cut(s) 2064
DriI GACNNNNNGTC 1 cut(s) 223
EaeI YGGCCR 2 cut(s) 32, 231
EagI CGGCCG 2 cut(s) 32, 231
Eam1104I CTCTTC 4 cut(s) 161, 758, 1939, 2553
Eam1105I GACNNNNNGTC 1 cut(s) 223
EarI CTCTTC 4 cut(s) 161, 758, 1939, 2553
Ecl136II GAGCTC 1 cut(s) 1525
EclXI CGGCCG 2 cut(s) 32, 231
Eco130I CCWWGG 1 cut(s) 2122
Eco24I GRGCYC 1 cut(s) 1527
Eco47I GGWCC 3 cut(s) 325, 1235, 2412
Eco47III AGCGCT 1 cut(s) 1008
Eco52I CGGCCG 2 cut(s) 32, 231
Eco53kI GAGCTC 1 cut(s) 1525
Eco57I CTGAAG 2 cut(s) 839, 1353
Eco72I CACGTG 2 cut(s) 532, 1655
Eco91I GGTNACC 2 cut(s) 577, 779
EcoICRI GAGCTC 1 cut(s) 1525
EcoO109I RGGNCCY 1 cut(s) 1220
EcoO65I GGTNACC 2 cut(s) 577, 779
EcoRII CCWGG 3 cut(s) 219, 369, 2802
EcoT14I CCWWGG 1 cut(s) 2122
EcoT22I ATGCAT 3 cut(s) 727, 1585, 2680
EcoT38I GRGCYC 1 cut(s) 1527
ErhI CCWWGG 1 cut(s) 2122
Esp3I CGTCTC 1 cut(s) 2827
FalI AAGNNNNNCTT 6 cut(s) 164, 196, 1458, 1490, 2058, 2090
FaqI GGGAC 4 cut(s) 160, 311, 1248, 1943
FauI CCCGC 4 cut(s) 185, 856, 1882, 2556
FauNDI CATATG 1 cut(s) 1890
FbaI TGATCA 2 cut(s) 2050, 2695
FblI GTMKAC 1 cut(s) 2777
FokI GGATG 8 cut(s) 185, 692, 918, 1064, 1337, 2105, 2377, 2687
FriOI GRGCYC 1 cut(s) 1527
FspBI CTAG 5 cut(s) 441, 1412, 2468, 2510, 2669
FspI TGCGCA 1 cut(s) 435
GlaI GCGC 4 cut(s) 435, 891, 1008, 1479
GsuI CTGGAG 6 cut(s) 94, 205, 242, 1155, 1817, 2646
HaeII RGCGCY 1 cut(s) 1010
HaeIII GGCC 7 cut(s) 34, 233, 916, 1221, 1928, 2263, 2830
HapII CCGG 6 cut(s) 31, 99, 230, 323, 784, 875
HgaI GACGC 1 cut(s) 84
HhaI GCGC 4 cut(s) 436, 892, 1009, 1480
Hin1I GRCGYC 1 cut(s) 95
Hin6I GCGC 4 cut(s) 434, 890, 1007, 1478
HinP1I GCGC 4 cut(s) 434, 890, 1007, 1478
HincII GTYRAC 2 cut(s) 2143, 2854
HindII GTYRAC 2 cut(s) 2143, 2854
HindIII AAGCTT 4 cut(s) 380, 1106, 1472, 2072
HpaI GTTAAC 1 cut(s) 2854
HpaII CCGG 6 cut(s) 31, 99, 230, 323, 784, 875
HphI GGTGA 9 cut(s) 253, 535, 589, 640, 647, 1391, 1553, 1707, 2138
Hpy166II GTNNAC 6 cut(s) 411, 1001, 2129, 2143, 2778, 2854
Hpy8I GTNNAC 6 cut(s) 411, 1001, 2129, 2143, 2778, 2854
Hpy99I CGWCG 3 cut(s) 30, 281, 359
HpyCH4III ACNGT 9 cut(s) 883, 979, 1077, 1648, 1996, 2133, 2333, 2688, 2738
HpyCH4IV ACGT 3 cut(s) 276, 531, 1654
HpyF10VI GCNNNNNNNGC 9 cut(s) 36, 186, 947, 1580, 1733, 1946, 2279, 2282, 2714
HpyF3I CTNAG 5 cut(s) 390, 1271, 1623, 1866, 1905
HpySE526I ACGT 3 cut(s) 276, 531, 1654
Hsp92I GRCGYC 1 cut(s) 95
HspAI GCGC 4 cut(s) 434, 890, 1007, 1478
Kpn2I TCCGGA 2 cut(s) 98, 874
KroI GCCGGC 1 cut(s) 30
KroNI GCCGGC 1 cut(s) 32
Ksp22I TGATCA 2 cut(s) 2050, 2695
KspAI GTTAAC 1 cut(s) 2854
LguI GCTCTTC 2 cut(s) 1939, 2553
LmnI GCTCC 6 cut(s) 186, 394, 499, 1530, 2448, 2518
Lsp1109I GCAGC 6 cut(s) 874, 1964, 2248, 2260, 2269, 2272
LweI GCATC 8 cut(s) 517, 828, 940, 1339, 1570, 2635, 2665, 2687
MaeI CTAG 5 cut(s) 441, 1412, 2468, 2510, 2669
MaeII ACGT 3 cut(s) 276, 531, 1654
MaeIII GTNAC 6 cut(s) 272, 527, 577, 779, 958, 1857
MbiI CCGCTC 3 cut(s) 403, 1949, 2563
MfeI CAATTG 4 cut(s) 1296, 1808, 2352, 2439
MflI RGATCY 3 cut(s) 101, 1909, 2566
MhlI GDGCHC 1 cut(s) 1527
MlyI GAGTC 8 cut(s) 200, 233, 317, 535, 752, 866, 1836, 2483
MmeI TCCRAC 2 cut(s) 328, 777
Mph1103I ATGCAT 3 cut(s) 727, 1585, 2680
MroI TCCGGA 2 cut(s) 98, 874
MroNI GCCGGC 1 cut(s) 30
MseI TTAA 9 cut(s) 771, 903, 1586, 1803, 1974, 2063, 2574, 2825, 2853
MslI CAYNNNNRTG 5 cut(s) 737, 1677, 1746, 2340, 2654
MspA1I CMGCKG 2 cut(s) 1241, 1955
MspI CCGG 6 cut(s) 31, 99, 230, 323, 784, 875
MspR9I CCNGG 3 cut(s) 221, 371, 2804
MunI CAATTG 4 cut(s) 1296, 1808, 2352, 2439
Mva1269I GAATGC 2 cut(s) 1736, 2661
MvaI CCWGG 3 cut(s) 221, 371, 2804
MvnI CGCG 1 cut(s) 239
MwoI GCNNNNNNNGC 9 cut(s) 36, 186, 947, 1580, 1733, 1946, 2279, 2282, 2714
NaeI GCCGGC 1 cut(s) 32
NcoI CCATGG 1 cut(s) 2122
NdeI CATATG 1 cut(s) 1890
NgoMIV GCCGGC 1 cut(s) 30
NlaIV GGNNCC 7 cut(s) 327, 368, 447, 873, 1236, 1911, 2414
NmeAIII GCCGAG 1 cut(s) 2856
NmuCI GTSAC 3 cut(s) 527, 577, 958
NsbI TGCGCA 1 cut(s) 435
NsiI ATGCAT 3 cut(s) 727, 1585, 2680
NspI RCATGY 1 cut(s) 2762
PciI ACATGT 1 cut(s) 2758
PciSI GCTCTTC 2 cut(s) 1939, 2553
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 351
PctI GAATGC 2 cut(s) 1736, 2661
PdiI GCCGGC 1 cut(s) 32
PfeI GAWTC 5 cut(s) 351, 559, 745, 800, 2012
PflMI CCANNNNNTGG 1 cut(s) 1244
PleI GAGTC 8 cut(s) 199, 232, 316, 534, 752, 865, 1836, 2482
PmaCI CACGTG 2 cut(s) 532, 1655
PmlI CACGTG 2 cut(s) 532, 1655
PpsI GAGTC 8 cut(s) 199, 232, 316, 534, 752, 865, 1836, 2482
Ppu21I YACGTR 2 cut(s) 532, 1655
PscI ACATGT 1 cut(s) 2758
Psp124BI GAGCTC 1 cut(s) 1527
Psp6I CCWGG 3 cut(s) 219, 369, 2802
PspCI CACGTG 2 cut(s) 532, 1655
PspEI GGTNACC 2 cut(s) 577, 779
PspGI CCWGG 3 cut(s) 219, 369, 2802
PspN4I GGNNCC 7 cut(s) 327, 368, 447, 873, 1236, 1911, 2414
PspPI GGNCC 5 cut(s) 325, 1220, 1235, 2262, 2412
PstNI CAGNNNCTG 1 cut(s) 2044
PsuI RGATCY 3 cut(s) 101, 1909, 2566
PvuII CAGCTG 2 cut(s) 1241, 1955
RsaI GTAC 8 cut(s) 317, 989, 1074, 1287, 2524, 2582, 2740, 2757
RsaNI GTAC 8 cut(s) 316, 988, 1073, 1286, 2523, 2581, 2739, 2756
RseI CAYNNNNRTG 5 cut(s) 737, 1677, 1746, 2340, 2654
SacI GAGCTC 1 cut(s) 1527
SapI GCTCTTC 2 cut(s) 1939, 2553
SaqAI TTAA 9 cut(s) 771, 903, 1586, 1803, 1974, 2063, 2574, 2825, 2853
Sau96I GGNCC 5 cut(s) 325, 1220, 1235, 2262, 2412
SchI GAGTC 8 cut(s) 200, 233, 317, 535, 752, 866, 1836, 2483
ScrFI CCNGG 3 cut(s) 221, 371, 2804
SduI GDGCHC 1 cut(s) 1527
SfaNI GCATC 8 cut(s) 517, 828, 940, 1339, 1570, 2635, 2665, 2687
SfcI CTRYAG 2 cut(s) 1575, 2712
SinI GGWCC 3 cut(s) 325, 1235, 2412
SmiMI CAYNNNNRTG 5 cut(s) 737, 1677, 1746, 2340, 2654
SmlI CTYRAG 6 cut(s) 164, 398, 710, 1210, 1823, 2403
SmoI CTYRAG 6 cut(s) 164, 398, 710, 1210, 1823, 2403
SspMI CTAG 5 cut(s) 441, 1412, 2468, 2510, 2669
SstI GAGCTC 1 cut(s) 1527
StyD4I CCNGG 3 cut(s) 219, 369, 2802
StyI CCWWGG 1 cut(s) 2122
TaaI ACNGT 9 cut(s) 883, 979, 1077, 1648, 1996, 2133, 2333, 2688, 2738
TaiI ACGT 3 cut(s) 279, 534, 1657
TaqI TCGA 6 cut(s) 354, 584, 591, 616, 761, 2542
TaqII GACCGA 1 cut(s) 133
TatI WGTACW 4 cut(s) 1285, 2580, 2738, 2755
TauI GCSGC 5 cut(s) 50, 236, 239, 269, 1952
TfiI GAWTC 5 cut(s) 351, 559, 745, 800, 2012
Tru1I TTAA 9 cut(s) 771, 903, 1586, 1803, 1974, 2063, 2574, 2825, 2853
Tru9I TTAA 9 cut(s) 771, 903, 1586, 1803, 1974, 2063, 2574, 2825, 2853
TscAI CASTG 3 cut(s) 2100, 2231, 2741
TseFI GTSAC 3 cut(s) 527, 577, 958
TseI GCWGC 6 cut(s) 887, 1952, 2236, 2273, 2282, 2285
Tsp45I GTSAC 3 cut(s) 527, 577, 958
TspGWI ACGGA 4 cut(s) 184, 977, 1711, 1854
TspRI CASTG 3 cut(s) 2100, 2231, 2741
Van91I CCANNNNNTGG 1 cut(s) 1244
VpaK11BI GGWCC 3 cut(s) 325, 1235, 2412
XapI RAATTY 3 cut(s) 334, 1606, 1631
XbaI TCTAGA 1 cut(s) 2467
XceI RCATGY 1 cut(s) 2762
XmiI GTMKAC 1 cut(s) 2777
XspI CTAG 5 cut(s) 441, 1412, 2468, 2510, 2669
Zsp2I ATGCAT 3 cut(s) 727, 1585, 2680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.