MD04G1001000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015985
Physical Location & Seq
Forward (+)
113004 .. 133570
20567 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1001000.v1.1.491

Sequence Viewer

Length: 2856 bp
ATGGATTCTTGTCTTCGTCTTTGTTTTCGTCGGGGATCGCTTCCTCCTCTGCCTTCAATCATCCTCATGATTTCCACTTTTTTACTGTTCTTGGCTTCTGCATCTGCTGGATCTCCCTCTGGAATTGGTAAAAGCAGCAAGTCATCAGTCTTCTCTTTATTTAACCTTAAAGAGAAGAGTAGGTTTTGGAGTGAGGCTGTCATACGTGGTGATTTTGATGATTTGGAATCTTCCAGTCCCAGAAAAAAGGGTGTTGCCAACTTCACTAATGCAGGGAATATTGCAAATTATCTCAAGCTTCTGGAAGTTGATTCTATGTACCTCCCTGTCCCTGTGAATTTCATTTTTATTGGATTTGATGGGAAAGGAAATCAAGGTTTCAAGCTTCACCCTGAGGAACTTGAGCGGTGGTTTACAAAAATTGATCATATCTTTGAACATACACGGGTTCCCCAAATTGGAGAAGTCCTCACTCCGTTTTACAGGATTAGTGTTGATAAAGAACAACGACACCATCTTCCCATTGTCAGTCACATAAACTACAATTTTTCTGTTCATGCAATACAAATGGGTGAAAAGGTTACTTCAATATTTGAGAAAGCCATAGATGTTTTCTCTCGTCAGGATGATGCATATGGTAACAGGGATGGCAGTGGTGTTTTGTGGCAAGTAGATGTGGACATGATGGATGTTCTTTTCACTAGCCTTGTTGGATATCTTGAACTTGAAAATGCATATAATGTTTTCATCTTGAATCCCAAGCATGACTCAAAAAAAGCTAAATATGGTTACCGGAGAGGTTTATCGGAGTCAGAGATAAAATTTCTCAAAGAGAACAAGAATTTGCAAACCAAGATTCTTCAGTCGGGAAGCATTCCGGAAACTGTTCTTGCTCTTGATAAGATAAAGAGACCTTTGTACGAGAAGCATCCAATGGCAAAGTTTGCGTGGTCTGTAACTGAAGACACTGATACAGTGGAATGGTACAATGCATGCCAAGAGGCATTGAATAATGTGGCAAAGTTGTATCAAGGAAAGGAAACTGCGGAAATCGTGCAGAACAAAGTTTTACAGTTATTGAAAGGGAAGAATGAAGATATGAAGCTTCTTTTCAGTAAGGAACTAAAATCTGGAGATGCGAATGTTCTTCATGGTGAATGTCTTACAGATACATGGATTGGAAAGGAGAGGTGGGCTTTTATTGATTTAAGTGCAGGTCCTTTCTCGTGGGGACCAGCTGTTGGTGGGGAAGGTGTTCGGACAGAACTAAGTTCTCCCAATGTGCAAAAAACGATTGGTGCAGTTTCAGAAATTTCAGAAGATGAAGCAGAAGATCGCTTACAAGATGCTATTCAGGAAAAATTTGCTGTATTTGGTGATAAAGATCATCAGGCCATTGATATTCTTCTTGCGGAGATTGATATATACGAGCTTTTTGCTTTCAAACACTGCAAAGGCAGGAAAGTAAAGCTTGCTCTGTGTGAAGAACTTGATGAGAGAATGAGGGACTTGAAAAATGAGCTCCAATCATTTGAAGGTGAAGAATATGACGAAAGTCATAAGAGAAAGGCAATAGAGGCATTAAAGAGAATGGAGAATTGGAATTTATTCAGTGATACTCATGAGGAGTTTCAAAATTACACAGTTGCACGTGATACTTTTCTTTCACATTTAGGTGCAACTTTGTGGGGGTCTATGAGACACATCATATCACCTTCCATCGCAGATGGTGCATTTCATTATTATGATGAGATATCATTTCAGTTATTTTTCATCACACAGGAGAAAGTTGGACACATTAAACAGTTGCCTGTGGACCTCAAGGCTCTTATGGATGGACTTTCCTCTTTGTTGTTACCCTCTCAAAAACCTGCTTTCAGTCAGCACTTGTTACCACTTTCAGAAGATCCTGCTTTGGCAATGGCCTTTTCAGTGGCACGAAGAGCTGCAGCTGTACCACTGTTGCTAGTTAATGGAACTTACAGGAAATCTGTCCGTACCTATCTCGATTCCTCCATTGTTCAGCATCAGTTGCAGAGACTGAATGATCATGGCTCACTAAAAGGAAAGCTTGCCCATAGCCGGTCTACACTAGAAGTTCCAATTTTTTGGTTCATTCATGGTGAACCATTGTTGGTGGACAAGCATTATCAGGCAAAGGCTCTTTATGATATGGTTATTGTTGTTCAGTCAGAGCCTTCATCCTGGGAAAGCCATCTGCAATGTAATGGGCAGCCACTTTTGTGGGATTTGAGGAGGCCCATCAAAGCTGCTTTGGCTGCTGCTTCTGAACATCTAGCTGGTTTGCTTCCCCTTCATCTAGCTTACAGTCAAGCCCATGAAACTGCAATTGAGGACTGGATGTGGTCAGTCGGATGCAACCCGTATTCCATCACTTCTCAAGGCTGGAACATTTCACAATTTCAGTCTGATACAATTGCTCGGAGCTATATTATCACGACTCTGGAAGAGTCAATACAAATGGTCAATTCAGCCATTCATCTTCTAGTCATGGAGCATACAACTGAAAAAACCTTTGAACTTGTTCAGTCTCAGGAGGGCGAGCTAGTTAACAAGTATAATTATGTTGTCAGCCGGTGGAAAAGTATATCAACTGTTACGGGAGAATTGCGATATGCGGATGCCATGAGACTTCTATATACCTTAGAGGATGCCTCAAAAGGGTTTGTAGATAAAGTAAATACAACCATCGCTGTTCTCCACCCAATCCACTGCACAAGAGAGAGAAAAGTGCACGTGGTGTTTAATTTGAGTACGATTCCTGCTTACTTGGTTGTGCTTGGTGTACTGTATCTTGTATTAAGGCCAAGACGACCAAAGCCAAAAATCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

952

Amino Acids

107.83

Weight (kDa)

6.19

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7906 PF25483 379 - 431 2.8e-10 Domain of unknown function (DUF7906)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58100 AT5G58100
fragaria_vesca FvH4_4g03800
malus_domestica MD04G1001000.v1.1
prunus_persica Prupe.1G001000_v2.0.a1
pyrus_communis pycom04g00160
rosa_chinensis RchiOBHm_Chr4g0393651
rosa_laevigata RLG00000009738
rosa_multiflora Rmu_sc0010124.1_g000004
rosa_roxburghii Rroxscaffold_5G00338820
rosa_rugosa Rorug03G0371900.1
rosa_samantha Rh4AG048400 Rh4BG044800 Rh4CG051800
rosa_wichuraiana Rw4G003840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1205, 1879
AccB7I CCANNNNNTGG 1 cut(s) 1241
AccBSI CCGCTC 1 cut(s) 406
AccI GTMKAC 1 cut(s) 2087
AccIII TCCGGA 1 cut(s) 877
AciI CCGC 4 cut(s) 406, 1046, 1412, 2639
AclWI GGATC 3 cut(s) 43, 118, 1901
AcsI RAATTY 6 cut(s) 337, 821, 841, 1311, 1361, 1603
AcuI CTGAAG 2 cut(s) 845, 981
AcvI CACGTG 2 cut(s) 1652, 2758
AdeI CACNNNGTG 1 cut(s) 2761
AfaI GTAC 7 cut(s) 320, 920, 986, 1956, 1999, 2776, 2808
AfiI CCNNNNNNNGG 4 cut(s) 458, 1241, 2082, 2682
AjnI CCWGG 1 cut(s) 2204
AjuI GAANNNNNNNTTGG 2 cut(s) 1582, 1614
AleI CACNNNNGTG 1 cut(s) 2242
Alw21I GWGCWC 2 cut(s) 1524, 2757
Alw26I GTCTC 5 cut(s) 904, 1693, 2032, 2556, 2644
Alw44I GTGCAC 1 cut(s) 2753
AlwI GGATC 3 cut(s) 43, 118, 1901
AlwNI CAGNNNCTG 1 cut(s) 2041
Aor13HI TCCGGA 1 cut(s) 877
AoxI GGCC 4 cut(s) 1392, 1923, 2258, 2825
ApaLI GTGCAC 1 cut(s) 2753
ApeKI GCWGC 7 cut(s) 135, 1946, 1949, 2233, 2270, 2279, 2282
ApoI RAATTY 6 cut(s) 337, 821, 841, 1311, 1361, 1603
Asp700I GAANNNNTTC 4 cut(s) 885, 1254, 1607, 2544
AspS9I GGNCC 4 cut(s) 1217, 1232, 1816, 2259
AsuHPI GGTGA 8 cut(s) 221, 380, 584, 1166, 1388, 1550, 1704, 2135
AvaII GGWCC 3 cut(s) 1217, 1232, 1816
AxyI CCTNAGG 1 cut(s) 393
BaeGI GKGCMC 1 cut(s) 2757
BanII GRGCYC 1 cut(s) 1524
BarI GAAGNNNNNNTAC 4 cut(s) 1323, 1355, 2460, 2492
BauI CACGAG 1 cut(s) 1225
BbrPI CACGTG 2 cut(s) 1652, 2758
BbsI GAAGAC 3 cut(s) 5, 142, 969
Bbv12I GWGCWC 2 cut(s) 1524, 2757
BbvI GCAGC 7 cut(s) 147, 1933, 1961, 2245, 2257, 2266, 2269
BcgI CGANNNNNNTGC 2 cut(s) 1418, 1452
BciT130I CCWGG 1 cut(s) 2206
BclI TGATCA 2 cut(s) 424, 2047
BcoDI GTCTC 5 cut(s) 904, 1693, 2032, 2556, 2644
BfaI CTAG 7 cut(s) 702, 1967, 2093, 2297, 2321, 2507, 2567
BfmI CTRYAG 1 cut(s) 1947
BfuAI ACCTGC 2 cut(s) 1205, 1879
BisI GCNGC 7 cut(s) 136, 1947, 1950, 2234, 2271, 2280, 2283
BlsI GCNGC 7 cut(s) 137, 1948, 1951, 2235, 2272, 2281, 2284
Bme1390I CCNGG 1 cut(s) 2206
Bme18I GGWCC 3 cut(s) 1217, 1232, 1816
BmgT120I GGNCC 4 cut(s) 1217, 1232, 1816, 2259
BmiI GGNNCC 2 cut(s) 450, 1233
BmrFI CCNGG 1 cut(s) 2206
BmsI GCATC 9 cut(s) 110, 619, 937, 1126, 1336, 2035, 2366, 2632, 2662
BoxI GACNNNNGTC 1 cut(s) 1554
BpiI GAAGAC 3 cut(s) 5, 142, 969
BplI GAGNNNNNCTC 4 cut(s) 453, 485, 2660, 2692
BpmI CTGGAG 1 cut(s) 1152
BpuEI CTTGAG 4 cut(s) 278, 422, 1805, 2385
BsaAI YACGTR 3 cut(s) 206, 1652, 2758
BsaBI GATNNNNATC 1 cut(s) 1383
BsaI GGTCTC 1 cut(s) 904
BsaJI CCNNGG 1 cut(s) 2205
BsaWI WCCGGW 2 cut(s) 792, 877
Bsc4I CCNNNNNNNGG 4 cut(s) 458, 1241, 2082, 2682
Bse118I RCCGGY 2 cut(s) 2082, 2595
Bse1I ACTGG 2 cut(s) 234, 2363
Bse21I CCTNAGG 1 cut(s) 393
Bse3DI GCAATG 2 cut(s) 1926, 2228
Bse8I GATNNNNATC 1 cut(s) 1383
BseAI TCCGGA 1 cut(s) 877
BseBI CCWGG 1 cut(s) 2206
BseDI CCNNGG 1 cut(s) 2205
BseJI GATNNNNATC 1 cut(s) 1383
BseLI CCNNNNNNNGG 4 cut(s) 458, 1241, 2082, 2682
BseMI GCAATG 2 cut(s) 1926, 2228
BseMII CTCAG 2 cut(s) 384, 2567
BseNI ACTGG 2 cut(s) 234, 2363
BseRI GAGGAG 3 cut(s) 36, 1640, 2269
BseSI GKGCMC 1 cut(s) 2757
BseXI GCAGC 7 cut(s) 147, 1933, 1961, 2245, 2257, 2266, 2269
BsgI GTGCAG 4 cut(s) 1076, 1233, 1320, 2719
BshFI GGCC 4 cut(s) 1394, 1925, 2260, 2827
BsiHKAI GWGCWC 2 cut(s) 1524, 2757
BsiSI CCGG 4 cut(s) 793, 878, 2083, 2596
BslFI GGGAC 4 cut(s) 222, 314, 1245, 1520
BslI CCNNNNNNNGG 4 cut(s) 458, 1241, 2082, 2682
BsmAI GTCTC 5 cut(s) 904, 1693, 2032, 2556, 2644
BsmFI GGGAC 4 cut(s) 222, 314, 1245, 1520
BsmI GAATGC 1 cut(s) 873
BsnI GGCC 4 cut(s) 1394, 1925, 2260, 2827
Bso31I GGTCTC 1 cut(s) 904
Bsp1286I GDGCHC 2 cut(s) 1524, 2757
Bsp13I TCCGGA 1 cut(s) 877
Bsp143I GATC 7 cut(s) 35, 110, 424, 1333, 1384, 1906, 2047
BspACI CCGC 4 cut(s) 406, 1046, 1412, 2639
BspANI GGCC 4 cut(s) 1394, 1925, 2260, 2827
BspCNI CTCAG 2 cut(s) 385, 2566
BspEI TCCGGA 1 cut(s) 877
BspHI TCATGA 2 cut(s) 66, 1621
BspLI GGNNCC 2 cut(s) 450, 1233
BspMAI CTGCAG 1 cut(s) 1951
BspMI ACCTGC 2 cut(s) 1205, 1879
BspPI GGATC 3 cut(s) 43, 118, 1901
BspQI GCTCTTC 1 cut(s) 1936
BspTNI GGTCTC 1 cut(s) 904
BsrBI CCGCTC 1 cut(s) 406
BsrDI GCAATG 2 cut(s) 1926, 2228
BsrFI RCCGGY 2 cut(s) 2082, 2595
BsrI ACTGG 2 cut(s) 234, 2363
BssAI RCCGGY 2 cut(s) 2082, 2595
BssECI CCNNGG 1 cut(s) 2205
BssMI GATC 7 cut(s) 35, 110, 424, 1333, 1384, 1906, 2047
BssSI CACGAG 1 cut(s) 1225
Bst2BI CACGAG 1 cut(s) 1225
Bst2UI CCWGG 1 cut(s) 2206
Bst6I CTCTTC 3 cut(s) 170, 1936, 2463
BstAPI GCANNNNNTGC 3 cut(s) 944, 1730, 2032
BstBAI YACGTR 3 cut(s) 206, 1652, 2758
BstC8I GCNNGC 4 cut(s) 994, 1473, 2073, 2564
BstDEI CTNAG 4 cut(s) 393, 1268, 2553, 2665
BstEII GGTNACC 1 cut(s) 788
BstKTI GATC 7 cut(s) 38, 113, 427, 1336, 1387, 1909, 2050
BstMAI GTCTC 5 cut(s) 904, 1693, 2032, 2556, 2644
BstMBI GATC 7 cut(s) 35, 110, 424, 1333, 1384, 1906, 2047
BstMWI GCNNNNNNNGC 7 cut(s) 944, 1577, 1730, 1943, 2032, 2276, 2279
BstNI CCWGG 1 cut(s) 2206
BstNSI RCATGY 1 cut(s) 996
BstPAI GACNNNNGTC 1 cut(s) 1554
BstPI GGTNACC 1 cut(s) 788
BstSCI CCNGG 1 cut(s) 2204
BstSFI CTRYAG 1 cut(s) 1947
BstSLI GKGCMC 1 cut(s) 2757
BstV1I GCAGC 7 cut(s) 147, 1933, 1961, 2245, 2257, 2266, 2269
BstV2I GAAGAC 3 cut(s) 5, 142, 969
BstX2I RGATCY 2 cut(s) 110, 1906
BstXI CCANNNNNNTGG 2 cut(s) 2109, 2244
BstYI RGATCY 2 cut(s) 110, 1906
Bsu36I CCTNAGG 1 cut(s) 393
BsuRI GGCC 4 cut(s) 1394, 1925, 2260, 2827
BtgZI GCGATG 2 cut(s) 1705, 2695
BtsI GCAGTG 3 cut(s) 658, 1447, 2731
BtsIMutI CAGTG 8 cut(s) 658, 966, 981, 1447, 1618, 1938, 1958, 2731
BveI ACCTGC 2 cut(s) 1205, 1879
Cac8I GCNNGC 4 cut(s) 994, 1473, 2073, 2564
CaiI CAGNNNCTG 1 cut(s) 2041
CciI TCATGA 2 cut(s) 66, 1621
Cfr10I RCCGGY 2 cut(s) 2082, 2595
Cfr13I GGNCC 4 cut(s) 1217, 1232, 1816, 2259
Csp6I GTAC 7 cut(s) 319, 919, 985, 1955, 1998, 2775, 2807
CviQI GTAC 7 cut(s) 319, 919, 985, 1955, 1998, 2775, 2807
DdeI CTNAG 4 cut(s) 393, 1268, 2553, 2665
DpnI GATC 7 cut(s) 37, 112, 426, 1335, 1386, 1908, 2049
DpnII GATC 7 cut(s) 35, 110, 424, 1333, 1384, 1906, 2047
DraIII CACNNNGTG 1 cut(s) 2761
Eam1104I CTCTTC 3 cut(s) 170, 1936, 2463
EarI CTCTTC 3 cut(s) 170, 1936, 2463
Ecl136II GAGCTC 1 cut(s) 1522
Eco24I GRGCYC 1 cut(s) 1524
Eco31I GGTCTC 1 cut(s) 904
Eco32I GATATC 2 cut(s) 716, 1755
Eco47I GGWCC 3 cut(s) 1217, 1232, 1816
Eco53kI GAGCTC 1 cut(s) 1522
Eco57I CTGAAG 2 cut(s) 845, 981
Eco72I CACGTG 2 cut(s) 1652, 2758
Eco81I CCTNAGG 1 cut(s) 393
Eco91I GGTNACC 1 cut(s) 788
EcoICRI GAGCTC 1 cut(s) 1522
EcoO109I RGGNCCY 1 cut(s) 1217
EcoO65I GGTNACC 1 cut(s) 788
EcoRII CCWGG 1 cut(s) 2204
EcoRV GATATC 2 cut(s) 716, 1755
EcoT22I ATGCAT 3 cut(s) 634, 736, 994
EcoT38I GRGCYC 1 cut(s) 1524
FalI AAGNNNNNCTT 4 cut(s) 1455, 1487, 2055, 2087
FaqI GGGAC 4 cut(s) 222, 314, 1245, 1520
FauNDI CATATG 1 cut(s) 634
FbaI TGATCA 2 cut(s) 424, 2047
FblI GTMKAC 1 cut(s) 2087
Fnu4HI GCNGC 7 cut(s) 136, 1947, 1950, 2234, 2271, 2280, 2283
FriOI GRGCYC 1 cut(s) 1524
Fsp4HI GCNGC 7 cut(s) 136, 1947, 1950, 2234, 2271, 2280, 2283
FspBI CTAG 7 cut(s) 702, 1967, 2093, 2297, 2321, 2507, 2567
GluI GCNGC 7 cut(s) 136, 1947, 1950, 2234, 2271, 2280, 2283
GsuI CTGGAG 1 cut(s) 1152
HaeIII GGCC 4 cut(s) 1394, 1925, 2260, 2827
HapII CCGG 4 cut(s) 793, 878, 2083, 2596
HincII GTYRAC 1 cut(s) 2572
HindII GTYRAC 1 cut(s) 2572
HindIII AAGCTT 5 cut(s) 296, 383, 1103, 1469, 2069
HpaI GTTAAC 1 cut(s) 2572
HpaII CCGG 4 cut(s) 793, 878, 2083, 2596
HphI GGTGA 8 cut(s) 221, 380, 584, 1166, 1388, 1550, 1704, 2135
Hpy166II GTNNAC 9 cut(s) 414, 679, 1816, 2088, 2126, 2140, 2572, 2755, 2807
Hpy8I GTNNAC 9 cut(s) 414, 679, 1816, 2088, 2126, 2140, 2572, 2755, 2807
Hpy99I CGWCG 1 cut(s) 33
HpyAV CCTTC 6 cut(s) 63, 1244, 1529, 1725, 2208, 2324
HpyCH4IV ACGT 3 cut(s) 205, 1651, 2757
HpyF10VI GCNNNNNNNGC 7 cut(s) 944, 1577, 1730, 1943, 2032, 2276, 2279
HpyF3I CTNAG 4 cut(s) 393, 1268, 2553, 2665
HpySE526I ACGT 3 cut(s) 205, 1651, 2757
Kpn2I TCCGGA 1 cut(s) 877
Ksp22I TGATCA 2 cut(s) 424, 2047
KspAI GTTAAC 1 cut(s) 2572
Kzo9I GATC 7 cut(s) 35, 110, 424, 1333, 1384, 1906, 2047
LguI GCTCTTC 1 cut(s) 1936
LmnI GCTCC 3 cut(s) 1527, 2445, 2515
Lsp1109I GCAGC 7 cut(s) 147, 1933, 1961, 2245, 2257, 2266, 2269
LweI GCATC 9 cut(s) 110, 619, 937, 1126, 1336, 2035, 2366, 2632, 2662
MaeI CTAG 7 cut(s) 702, 1967, 2093, 2297, 2321, 2507, 2567
MaeII ACGT 3 cut(s) 205, 1651, 2757
MaeIII GTNAC 8 cut(s) 530, 580, 638, 788, 955, 1854, 1890, 2617
MalI GATC 7 cut(s) 37, 112, 426, 1335, 1386, 1908, 2049
MbiI CCGCTC 1 cut(s) 406
MboI GATC 7 cut(s) 35, 110, 424, 1333, 1384, 1906, 2047
MfeI CAATTG 3 cut(s) 2349, 2436, 2851
MflI RGATCY 2 cut(s) 110, 1906
MhlI GDGCHC 2 cut(s) 1524, 2757
MlyI GAGTC 4 cut(s) 761, 818, 2455, 2480
MmeI TCCRAC 3 cut(s) 691, 1771, 2353
Mph1103I ATGCAT 3 cut(s) 634, 736, 994
MroI TCCGGA 1 cut(s) 877
MroXI GAANNNNTTC 4 cut(s) 885, 1254, 1607, 2544
MseI TTAA 9 cut(s) 162, 168, 1208, 1583, 1800, 1971, 2571, 2766, 2822
MslI CAYNNNNRTG 4 cut(s) 65, 1674, 1743, 2242
MspA1I CMGCKG 2 cut(s) 1238, 1952
MspI CCGG 4 cut(s) 793, 878, 2083, 2596
MspR9I CCNGG 1 cut(s) 2206
MunI CAATTG 3 cut(s) 2349, 2436, 2851
Mva1269I GAATGC 1 cut(s) 873
MvaI CCWGG 1 cut(s) 2206
MwoI GCNNNNNNNGC 7 cut(s) 944, 1577, 1730, 1943, 2032, 2276, 2279
NdeI CATATG 1 cut(s) 634
NdeII GATC 7 cut(s) 35, 110, 424, 1333, 1384, 1906, 2047
NlaIV GGNNCC 2 cut(s) 450, 1233
NmuCI GTSAC 1 cut(s) 530
NsiI ATGCAT 3 cut(s) 634, 736, 994
NspI RCATGY 1 cut(s) 996
OliI CACNNNNGTG 1 cut(s) 2242
PaeI GCATGC 1 cut(s) 996
PagI TCATGA 2 cut(s) 66, 1621
PciSI GCTCTTC 1 cut(s) 1936
PctI GAATGC 1 cut(s) 873
PdmI GAANNNNTTC 4 cut(s) 885, 1254, 1607, 2544
PfeI GAWTC 7 cut(s) 5, 227, 311, 754, 856, 2009, 2779
PflMI CCANNNNNTGG 1 cut(s) 1241
PkrI GCNGC 7 cut(s) 137, 1948, 1951, 2235, 2272, 2281, 2284
PleI GAGTC 4 cut(s) 761, 817, 2455, 2479
PmaCI CACGTG 2 cut(s) 1652, 2758
PmlI CACGTG 2 cut(s) 1652, 2758
PpsI GAGTC 4 cut(s) 761, 817, 2455, 2479
Ppu21I YACGTR 3 cut(s) 206, 1652, 2758
PpuMI RGGWCCY 1 cut(s) 1217
PshAI GACNNNNGTC 1 cut(s) 1554
Psp124BI GAGCTC 1 cut(s) 1524
Psp5II RGGWCCY 1 cut(s) 1217
Psp6I CCWGG 1 cut(s) 2204
PspCI CACGTG 2 cut(s) 1652, 2758
PspEI GGTNACC 1 cut(s) 788
PspGI CCWGG 1 cut(s) 2204
PspN4I GGNNCC 2 cut(s) 450, 1233
PspPI GGNCC 4 cut(s) 1217, 1232, 1816, 2259
PspPPI RGGWCCY 1 cut(s) 1217
PstI CTGCAG 1 cut(s) 1951
PstNI CAGNNNCTG 1 cut(s) 2041
PsuI RGATCY 2 cut(s) 110, 1906
PvuII CAGCTG 2 cut(s) 1238, 1952
RsaI GTAC 7 cut(s) 320, 920, 986, 1956, 1999, 2776, 2808
RsaNI GTAC 7 cut(s) 319, 919, 985, 1955, 1998, 2775, 2807
RseI CAYNNNNRTG 4 cut(s) 65, 1674, 1743, 2242
SacI GAGCTC 1 cut(s) 1524
SapI GCTCTTC 1 cut(s) 1936
SaqAI TTAA 9 cut(s) 162, 168, 1208, 1583, 1800, 1971, 2571, 2766, 2822
SatI GCNGC 7 cut(s) 136, 1947, 1950, 2234, 2271, 2280, 2283
Sau3AI GATC 7 cut(s) 35, 110, 424, 1333, 1384, 1906, 2047
Sau96I GGNCC 4 cut(s) 1217, 1232, 1816, 2259
SchI GAGTC 4 cut(s) 761, 818, 2455, 2480
ScrFI CCNGG 1 cut(s) 2206
SduI GDGCHC 2 cut(s) 1524, 2757
SfaNI GCATC 9 cut(s) 110, 619, 937, 1126, 1336, 2035, 2366, 2632, 2662
SfcI CTRYAG 1 cut(s) 1947
SinI GGWCC 3 cut(s) 1217, 1232, 1816
SmiMI CAYNNNNRTG 4 cut(s) 65, 1674, 1743, 2242
SmlI CTYRAG 4 cut(s) 293, 401, 1820, 2400
SmoI CTYRAG 4 cut(s) 293, 401, 1820, 2400
SphI GCATGC 1 cut(s) 996
SsiI CCGC 4 cut(s) 406, 1046, 1412, 2639
SspI AATATT 2 cut(s) 280, 591
SspMI CTAG 7 cut(s) 702, 1967, 2093, 2297, 2321, 2507, 2567
SstI GAGCTC 1 cut(s) 1524
StyD4I CCNGG 1 cut(s) 2204
TaiI ACGT 3 cut(s) 208, 1654, 2760
TaqI TCGA 1 cut(s) 2007
TatI WGTACW 1 cut(s) 2806
TfiI GAWTC 7 cut(s) 5, 227, 311, 754, 856, 2009, 2779
Tru1I TTAA 9 cut(s) 162, 168, 1208, 1583, 1800, 1971, 2571, 2766, 2822
Tru9I TTAA 9 cut(s) 162, 168, 1208, 1583, 1800, 1971, 2571, 2766, 2822
TscAI CASTG 8 cut(s) 658, 973, 981, 1454, 1618, 1938, 1965, 2738
TseFI GTSAC 1 cut(s) 530
TseI GCWGC 7 cut(s) 135, 1946, 1949, 2233, 2270, 2279, 2282
Tsp45I GTSAC 1 cut(s) 530
TspGWI ACGGA 2 cut(s) 465, 1985
TspRI CASTG 8 cut(s) 658, 973, 981, 1454, 1618, 1938, 1965, 2738
Van91I CCANNNNNTGG 1 cut(s) 1241
VneI GTGCAC 1 cut(s) 2753
VpaK11BI GGWCC 3 cut(s) 1217, 1232, 1816
XapI RAATTY 6 cut(s) 337, 821, 841, 1311, 1361, 1603
XceI RCATGY 1 cut(s) 996
XmiI GTMKAC 1 cut(s) 2087
XmnI GAANNNNTTC 4 cut(s) 885, 1254, 1607, 2544
XspI CTAG 7 cut(s) 702, 1967, 2093, 2297, 2321, 2507, 2567
Zsp2I ATGCAT 3 cut(s) 634, 736, 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.