AT5G59790

Domain of unknown function (DUF966)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008643
Physical Location & Seq
Forward (+)
24090645 .. 24093168
2524 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G59790.1

Sequence Viewer

Length: 1272 bp
ATGAGTTCAAGAGTGTTCAGAGCAACACCAGATAACAACTATTTGGTCCCCAGGAGATCCAAAGACCAACAAGACACCAGTCCCGACCGTAACCGAATCTGGTCTGAGCCTCGTCTTAAACCCGTCGTTAACCGGAAAGTCCCAGTCGTCTATTATCTCTGCCGGAATGGTCAGCTTGACCATCCTCATTTCATCGAAGTCACTCTCTCTTCCCACGATGGTCTTTATCTCAAAGATGTGATAAACCGCTTGAATGATCTAAGAGGGAAAGGCATGGCAAGTCTCTACTCTTGGTCTTCGAAACGGAGCTACAAAAATGGATTTGTCTGGCACGATTTATCGGAGGACGATTTCATCTTCCCTGTTCAAGGTCAAGAATATGTTCTCAAAGGCTCTGAGGTTCTTGATTCTTGTCTTATCTCAAACCCAAGAAGCCTCCTCGAAACGTCGTCGTTTAGAGACCCAAGATCTTTAAATCCAGATAAAAACTCCGGCGATGATATCCCGGCGGTGATAAATCGACGGAGGAACCAGTCTTGGAGCTCTATCGATCTCAGCGAGTACAAAGTGTACAAAGCAACCGAGTCAAGCGCCGAGTCAACTCAAAGACTCGCTGCGGACGCTTCTACTCAGACTGACGATAGACGACGGAGGAGAAGACCGGCGAAAGAGGAAATAGAAGAGGTGAAAAGTCCGGCGAGTTATGAGAATCAGAGTACAGAGCTGAGCAGAGACGAGATATCTCCTCCACCGTCGGATTCAAGTCCCGAGACATTAGAGAATCTGATCAAAGCCGATGGACGGCTGATATTGCGTCCTAGCGAAAGCAGCACCGATCATCGGACGGTCGAGAGTTTATCCAGCGGAAGAATGAGAGCTTCGGCTGTGTTAATGCAGCTGATATCGTGTGGTACGATGTCGTTTAAAGAATGTGGGCCTGTTTTGTTGAAAGATCAAGGGCTGGCGTTGAACGGGCGTAGCGGGTGTACGATAACGCGAGGAGCAGAGGATAATGGTGAGGAGAGGGTAGATAAGGAATTAAAGAGTTTTGGAAGGGTACAATTGGAAGATAAGGAGTATTTTAGCGGCAGTTTGATTGAGACCAAGAAGGAGCTTGTTCCTGCTTTGAAAAGATCTTCTTCTTACAATGCTGACAGGTGTTCAAGAATGGGACCAACGACGGAGAAGGATGAGGAAGAAGCGGTTCGAGCTAAGTGCATTCCGAGAAAACCAAAGCCGGTGGCTAAAAGGAACAATGGTGGGCAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

423

Amino Acids

47.78

Weight (kDa)

8.68

Isoelectric Point (pI)

66.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOK PF06136 47 - 135 4.9e-50 SOSEKI protein DIX-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 997
AciI CCGC 7 cut(s) 247, 509, 617, 864, 981, 1086, 1202
AclWI GGATC 1 cut(s) 51
AfaI GTAC 6 cut(s) 563, 572, 718, 913, 988, 1059
AfiI CCNNNNNNNGG 3 cut(s) 368, 801, 840
AgsI TTSAA 8 cut(s) 9, 253, 368, 762, 949, 970, 1129, 1164
AjnI CCWGG 1 cut(s) 50
AluBI AGCT 8 cut(s) 175, 309, 543, 724, 878, 898, 1114, 1211
AluI AGCT 8 cut(s) 175, 309, 543, 724, 878, 898, 1114, 1211
Alw21I GWGCWC 1 cut(s) 545
Alw26I GTCTC 5 cut(s) 287, 453, 726, 764, 1094
AlwI GGATC 1 cut(s) 51
Ama87I CYCGRG 1 cut(s) 767
AoxI GGCC 1 cut(s) 935
ApeKI GCWGC 4 cut(s) 614, 828, 895, 1264
Asp700I GAANNNNTTC 2 cut(s) 381, 1203
AspLEI GCGC 1 cut(s) 593
AspS9I GGNCC 3 cut(s) 46, 935, 1172
AsuC2I CCSGG 1 cut(s) 506
AsuHPI GGTGA 3 cut(s) 523, 697, 1028
AsuII TTCGAA 1 cut(s) 299
AvaI CYCGRG 1 cut(s) 767
AvaII GGWCC 2 cut(s) 46, 1172
BanII GRGCYC 1 cut(s) 545
BbsI GAAGAC 2 cut(s) 288, 664
Bbv12I GWGCWC 1 cut(s) 545
BbvI GCAGC 3 cut(s) 601, 840, 907
BccI CCATC 3 cut(s) 189, 212, 791
BceAI ACGGC 1 cut(s) 818
BcgI CGANNNNNNTGC 2 cut(s) 1197, 1231
BciT130I CCWGG 1 cut(s) 52
BclI TGATCA 1 cut(s) 786
BcnI CCSGG 1 cut(s) 506
BcoDI GTCTC 5 cut(s) 287, 453, 726, 764, 1094
BfaI CTAG 1 cut(s) 819
BfoI RGCGCY 1 cut(s) 594
BglII AGATCT 2 cut(s) 467, 1133
BisI GCNGC 5 cut(s) 615, 829, 896, 1087, 1265
BlpI GCTNAGC 1 cut(s) 725
BlsI GCNGC 5 cut(s) 616, 830, 897, 1088, 1266
Bme1390I CCNGG 2 cut(s) 52, 506
Bme18I GGWCC 2 cut(s) 46, 1172
BmeT110I CYCGRG 1 cut(s) 767
BmgT120I GGNCC 3 cut(s) 46, 935, 1172
BmiI GGNNCC 3 cut(s) 48, 530, 1173
BmrFI CCNGG 2 cut(s) 52, 506
BmrI ACTGGG 1 cut(s) 137
BmuI ACTGGG 1 cut(s) 137
BoxI GACNNNNGTC 1 cut(s) 78
BpiI GAAGAC 2 cut(s) 288, 664
Bpu1102I GCTNAGC 1 cut(s) 725
Bpu14I TTCGAA 1 cut(s) 299
BpuMI CCSGG 1 cut(s) 506
Bsa29I ATCGAT 1 cut(s) 549
BsaI GGTCTC 2 cut(s) 453, 1094
BsaJI CCNNGG 1 cut(s) 50
BsaWI WCCGGW 1 cut(s) 132
Bsc4I CCNNNNNNNGG 3 cut(s) 368, 801, 840
Bse118I RCCGGY 2 cut(s) 661, 1237
Bse1I ACTGG 3 cut(s) 78, 143, 532
BseBI CCWGG 1 cut(s) 52
BseCI ATCGAT 1 cut(s) 549
BseDI CCNNGG 1 cut(s) 50
BseGI GGATG 2 cut(s) 181, 1195
BseLI CCNNNNNNNGG 3 cut(s) 368, 801, 840
BseMII CTCAG 5 cut(s) 96, 387, 568, 644, 716
BseNI ACTGG 3 cut(s) 78, 143, 532
BseRI GAGGAG 5 cut(s) 428, 667, 735, 1014, 1034
BseXI GCAGC 3 cut(s) 601, 840, 907
Bsh1236I CGCG 1 cut(s) 997
Bsh1285I CGRYCG 2 cut(s) 88, 849
BshFI GGCC 1 cut(s) 937
BshVI ATCGAT 1 cut(s) 549
BsiEI CGRYCG 2 cut(s) 88, 849
BsiHKAI GWGCWC 1 cut(s) 545
BsiHKCI CYCGRG 1 cut(s) 767
BsiSI CCGG 7 cut(s) 133, 163, 492, 506, 662, 695, 1238
BslFI GGGAC 5 cut(s) 32, 66, 125, 750, 1185
BslI CCNNNNNNNGG 3 cut(s) 368, 801, 840
BsmAI GTCTC 5 cut(s) 287, 453, 726, 764, 1094
BsmBI CGTCTC 1 cut(s) 726
BsmFI GGGAC 5 cut(s) 32, 66, 125, 750, 1185
BsmI GAATGC 1 cut(s) 1218
BsnI GGCC 1 cut(s) 937
Bso31I GGTCTC 2 cut(s) 453, 1094
BsoBI CYCGRG 1 cut(s) 767
Bsp119I TTCGAA 1 cut(s) 299
Bsp1286I GDGCHC 1 cut(s) 545
Bsp1407I TGTACA 1 cut(s) 570
Bsp143I GATC 8 cut(s) 56, 256, 467, 550, 786, 835, 952, 1133
Bsp1720I GCTNAGC 1 cut(s) 725
BspACI CCGC 7 cut(s) 247, 509, 617, 864, 981, 1086, 1202
BspANI GGCC 1 cut(s) 937
BspCNI CTCAG 5 cut(s) 97, 388, 567, 643, 717
BspDI ATCGAT 1 cut(s) 549
BspFNI CGCG 1 cut(s) 997
BspLI GGNNCC 3 cut(s) 48, 530, 1173
BspPI GGATC 1 cut(s) 51
BspT104I TTCGAA 1 cut(s) 299
BspTNI GGTCTC 2 cut(s) 453, 1094
BsrFI RCCGGY 2 cut(s) 661, 1237
BsrGI TGTACA 1 cut(s) 570
BsrI ACTGG 3 cut(s) 78, 143, 532
BssAI RCCGGY 2 cut(s) 661, 1237
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 8 cut(s) 56, 256, 467, 550, 786, 835, 952, 1133
Bst2UI CCWGG 1 cut(s) 52
Bst4CI ACNGT 3 cut(s) 89, 753, 847
Bst6I CTCTTC 2 cut(s) 214, 675
BstAUI TGTACA 1 cut(s) 570
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 963
BstDEI CTNAG 7 cut(s) 105, 260, 396, 554, 630, 725, 1212
BstENI CCTNNNNNAGG 1 cut(s) 366
BstF5I GGATG 2 cut(s) 181, 1195
BstFNI CGCG 1 cut(s) 997
BstH2I RGCGCY 1 cut(s) 594
BstHHI GCGC 1 cut(s) 593
BstKTI GATC 8 cut(s) 59, 259, 470, 553, 789, 838, 955, 1136
BstMAI GTCTC 5 cut(s) 287, 453, 726, 764, 1094
BstMBI GATC 8 cut(s) 56, 256, 467, 550, 786, 835, 952, 1133
BstMCI CGRYCG 2 cut(s) 88, 849
BstMWI GCNNNNNNNGC 4 cut(s) 620, 811, 828, 1208
BstNI CCWGG 1 cut(s) 52
BstPAI GACNNNNGTC 1 cut(s) 78
BstSCI CCNGG 2 cut(s) 50, 504
BstUI CGCG 1 cut(s) 997
BstV1I GCAGC 3 cut(s) 601, 840, 907
BstV2I GAAGAC 2 cut(s) 288, 664
BstX2I RGATCY 3 cut(s) 56, 467, 1133
BstYI RGATCY 3 cut(s) 56, 467, 1133
Bsu15I ATCGAT 1 cut(s) 549
BsuRI GGCC 1 cut(s) 937
BsuTUI ATCGAT 1 cut(s) 549
BtgZI GCGATG 1 cut(s) 510
BtsCI GGATG 2 cut(s) 181, 1195
Cac8I GCNNGC 1 cut(s) 963
CfoI GCGC 1 cut(s) 593
Cfr10I RCCGGY 2 cut(s) 661, 1237
Cfr13I GGNCC 3 cut(s) 46, 935, 1172
ClaI ATCGAT 1 cut(s) 549
CseI GACGC 2 cut(s) 629, 803
Csp6I GTAC 6 cut(s) 562, 571, 717, 912, 987, 1058
CspCI CAANNNNNGTGG 2 cut(s) 1221, 1256
CviAII CATG 1 cut(s) 274
CviQI GTAC 6 cut(s) 562, 571, 717, 912, 987, 1058
DdeI CTNAG 7 cut(s) 105, 260, 396, 554, 630, 725, 1212
DpnI GATC 8 cut(s) 58, 258, 469, 552, 788, 837, 954, 1135
DpnII GATC 8 cut(s) 56, 256, 467, 550, 786, 835, 952, 1133
DraI TTTAAA 2 cut(s) 474, 925
Eam1104I CTCTTC 2 cut(s) 214, 675
EarI CTCTTC 2 cut(s) 214, 675
Ecl136II GAGCTC 1 cut(s) 543
Eco24I GRGCYC 1 cut(s) 545
Eco31I GGTCTC 2 cut(s) 453, 1094
Eco32I GATATC 3 cut(s) 502, 741, 903
Eco47I GGWCC 2 cut(s) 46, 1172
Eco53kI GAGCTC 1 cut(s) 543
Eco88I CYCGRG 1 cut(s) 767
EcoICRI GAGCTC 1 cut(s) 543
EcoNI CCTNNNNNAGG 1 cut(s) 366
EcoRII CCWGG 1 cut(s) 50
EcoRV GATATC 3 cut(s) 502, 741, 903
EcoT38I GRGCYC 1 cut(s) 545
Esp3I CGTCTC 1 cut(s) 726
FaeI CATG 1 cut(s) 277
FaiI YATR 3 cut(s) 275, 381, 705
FalI AAGNNNNNCTT 2 cut(s) 1123, 1155
FaqI GGGAC 5 cut(s) 32, 66, 125, 750, 1185
FatI CATG 1 cut(s) 273
FauI CCCGC 1 cut(s) 974
FbaI TGATCA 1 cut(s) 786
Fnu4HI GCNGC 5 cut(s) 615, 829, 896, 1087, 1265
FokI GGATG 2 cut(s) 168, 1202
FriOI GRGCYC 1 cut(s) 545
Fsp4HI GCNGC 5 cut(s) 615, 829, 896, 1087, 1265
FspBI CTAG 1 cut(s) 819
GlaI GCGC 1 cut(s) 592
GluI GCNGC 5 cut(s) 615, 829, 896, 1087, 1265
HaeII RGCGCY 1 cut(s) 594
HaeIII GGCC 1 cut(s) 937
HapII CCGG 7 cut(s) 133, 163, 492, 506, 662, 695, 1238
HgaI GACGC 2 cut(s) 629, 803
HhaI GCGC 1 cut(s) 593
Hin1II CATG 1 cut(s) 277
Hin6I GCGC 1 cut(s) 591
HinP1I GCGC 1 cut(s) 591
HincII GTYRAC 2 cut(s) 130, 600
HindII GTYRAC 2 cut(s) 130, 600
HinfI GANTC 8 cut(s) 96, 407, 584, 596, 609, 709, 758, 781
HpaI GTTAAC 1 cut(s) 130
HpaII CCGG 7 cut(s) 133, 163, 492, 506, 662, 695, 1238
HphI GGTGA 3 cut(s) 523, 697, 1028
Hpy166II GTNNAC 4 cut(s) 130, 571, 600, 987
Hpy188III TCNNGA 8 cut(s) 9, 83, 374, 404, 479, 767, 850, 1164
Hpy8I GTNNAC 4 cut(s) 130, 571, 600, 987
Hpy99I CGWCG 7 cut(s) 128, 451, 454, 525, 651, 757, 1183
HpyAV CCTTC 3 cut(s) 1047, 1102, 1180
HpyCH4III ACNGT 3 cut(s) 89, 753, 847
HpyCH4IV ACGT 1 cut(s) 446
HpyCH4V TGCA 2 cut(s) 895, 1218
HpyF10VI GCNNNNNNNGC 4 cut(s) 620, 811, 828, 1208
HpyF3I CTNAG 7 cut(s) 105, 260, 396, 554, 630, 725, 1212
HpySE526I ACGT 1 cut(s) 446
Hsp92II CATG 1 cut(s) 277
HspAI GCGC 1 cut(s) 591
Ksp22I TGATCA 1 cut(s) 786
KspAI GTTAAC 1 cut(s) 130
Kzo9I GATC 8 cut(s) 56, 256, 467, 550, 786, 835, 952, 1133
LmnI GCTCC 4 cut(s) 306, 540, 1001, 1111
Lsp1109I GCAGC 3 cut(s) 601, 840, 907
MaeI CTAG 1 cut(s) 819
MaeII ACGT 1 cut(s) 446
MaeIII GTNAC 2 cut(s) 89, 199
MalI GATC 8 cut(s) 58, 258, 469, 552, 788, 837, 954, 1135
MboI GATC 8 cut(s) 56, 256, 467, 550, 786, 835, 952, 1133
MfeI CAATTG 1 cut(s) 1061
MflI RGATCY 3 cut(s) 56, 467, 1133
MhlI GDGCHC 1 cut(s) 545
MluCI AATT 2 cut(s) 1037, 1061
MlyI GAGTC 3 cut(s) 593, 603, 605
MmeI TCCRAC 1 cut(s) 735
MroXI GAANNNNTTC 2 cut(s) 381, 1203
MseI TTAA 6 cut(s) 117, 129, 473, 890, 924, 1040
MspA1I CMGCKG 2 cut(s) 864, 898
MspI CCGG 7 cut(s) 133, 163, 492, 506, 662, 695, 1238
MspR9I CCNGG 2 cut(s) 52, 506
MunI CAATTG 1 cut(s) 1061
Mva1269I GAATGC 1 cut(s) 1218
MvaI CCWGG 1 cut(s) 52
MvnI CGCG 1 cut(s) 997
MwoI GCNNNNNNNGC 4 cut(s) 620, 811, 828, 1208
NciI CCSGG 1 cut(s) 506
NdeII GATC 8 cut(s) 56, 256, 467, 550, 786, 835, 952, 1133
NlaIII CATG 1 cut(s) 277
NlaIV GGNNCC 3 cut(s) 48, 530, 1173
NmeAIII GCCGAG 1 cut(s) 619
NmuCI GTSAC 1 cut(s) 199
NspV TTCGAA 1 cut(s) 299
PcsI WCGNNNNNNNCGW 2 cut(s) 555, 911
PctI GAATGC 1 cut(s) 1218
PdmI GAANNNNTTC 2 cut(s) 381, 1203
PfeI GAWTC 5 cut(s) 96, 407, 709, 758, 781
PkrI GCNGC 5 cut(s) 616, 830, 897, 1088, 1266
PleI GAGTC 3 cut(s) 592, 603, 604
PpsI GAGTC 3 cut(s) 592, 603, 604
PshAI GACNNNNGTC 1 cut(s) 78
Psp124BI GAGCTC 1 cut(s) 545
Psp6I CCWGG 1 cut(s) 50
PspGI CCWGG 1 cut(s) 50
PspN4I GGNNCC 3 cut(s) 48, 530, 1173
PspPI GGNCC 3 cut(s) 46, 935, 1172
PsuI RGATCY 3 cut(s) 56, 467, 1133
PvuII CAGCTG 1 cut(s) 898
RsaI GTAC 6 cut(s) 563, 572, 718, 913, 988, 1059
RsaNI GTAC 6 cut(s) 562, 571, 717, 912, 987, 1058
SacI GAGCTC 1 cut(s) 545
SaqAI TTAA 6 cut(s) 117, 129, 473, 890, 924, 1040
SatI GCNGC 5 cut(s) 615, 829, 896, 1087, 1265
Sau3AI GATC 8 cut(s) 56, 256, 467, 550, 786, 835, 952, 1133
Sau96I GGNCC 3 cut(s) 46, 935, 1172
SchI GAGTC 3 cut(s) 593, 603, 605
ScrFI CCNGG 2 cut(s) 52, 506
SduI GDGCHC 1 cut(s) 545
SfuI TTCGAA 1 cut(s) 299
SinI GGWCC 2 cut(s) 46, 1172
Sse9I AATT 2 cut(s) 1037, 1061
SsiI CCGC 7 cut(s) 247, 509, 617, 864, 981, 1086, 1202
SspMI CTAG 1 cut(s) 819
SstI GAGCTC 1 cut(s) 545
StyD4I CCNGG 2 cut(s) 50, 504
TaaI ACNGT 3 cut(s) 89, 753, 847
TaiI ACGT 1 cut(s) 449
TaqI TCGA 7 cut(s) 195, 299, 441, 520, 549, 849, 1207
TasI AATT 2 cut(s) 1037, 1061
TatI WGTACW 3 cut(s) 561, 570, 716
TauI GCSGC 1 cut(s) 1089
TfiI GAWTC 5 cut(s) 96, 407, 709, 758, 781
Tru1I TTAA 6 cut(s) 117, 129, 473, 890, 924, 1040
Tru9I TTAA 6 cut(s) 117, 129, 473, 890, 924, 1040
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 4 cut(s) 614, 828, 895, 1264
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 2 cut(s) 181, 343
TspGWI ACGGA 4 cut(s) 319, 538, 664, 1196
VpaK11BI GGWCC 2 cut(s) 46, 1172
XagI CCTNNNNNAGG 1 cut(s) 366
XmnI GAANNNNTTC 2 cut(s) 381, 1203
XspI CTAG 1 cut(s) 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.