AT5G61865

protein import into mitochondrial matrix

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012425
Physical Location & Seq
Forward (+)
24847310 .. 24850118
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G61865.1

Sequence Viewer

Length: 1254 bp
ATGGAGTTCCCGCCAAGAGCGTTTTATCCACGGAGATTCCAGGCCTCTCCGGCGCAATACACGGCGGAGGAAAGTGCTGCAGCAGTATCGGCGCCAGATCTCCGAGATAAGCTCGCGACACTGGTCGATCGCACCAAGAAAGTGAAAATGGCGTACCATCAACTCCAATCGCAGATCGCGTTCGGCCTTGTCGAAGCAGGTGAAGTTTTCGATTCTCTGGCGATCCCTTTGATGAAGTTGGTTGGGCTGAAAACGTCGGAGATGGAGAGTGAAGGGAGACACTCCGCCTTTATTTTCAATACTGAAAGGTATCATCATATGGATACTGCACGGTCTGGTGATCTGAACATTCAAATTCGCAGTTCAAAGGAAGAAAACTATGCTGCTAAAGCCGAAACAGCGGGGAAAGAGATCTTGCACAAGCATAAGGGGCAGCTGAGACAGTTAGTTCATATGCTTAAGCACATTGAAACACAGGTGAATTCACATCGGGAAGATATTCTGCAGATGGTTGACGATCGTAGAACCTTCTTCCAAGAATTCATCCAGAAGTCTCTTTATTACCTAAGCTCGGTTCACAGCCAAAACCATGACACTTTCCCTGTCACAGTGAAGCTCCTTCGCGTACTATTTAATAACATCAACGAGGTGCTTGGTTCAGTAGATACTGGAGTCAATGATTTTATGCAGGCTTTAGCAAAGAATATGTGTCACCCGATGACCAAGTACGTAGGCAACTTGGCTGCTGAAATAAAGCATGGCCCTTGTGTGCAACTGATGAATGTAGTGAATGAGATGGAGAGATCAAACTCAGATACAAGGAGAGAGCTACAAGATGCTAGAGAAAGGATTAGACTTGCAGAAGAGACAAAGATCAACGCTTTATCAAAGCTGAAAAAAGCTGAAGATCAAGTTCAGAGGATGACAACATCTGCCAAATTTCTTCTACCCGCGAGTCAGAAGAAACAAGCTGAACATTCTATTACCGGCAAGCGTATAGGCACAGAGGGAAGCCGAGAACACGAAGAGAAACTACTTTGGGAACTACTGAGCAAAAGGAGAAAGCAACAAGAGCCAGAGAGTCCAATGGGACCTAAAGAACTCATCAGACAATCAGACACAAAGCAGAAGACATTGGTGTACAATCGCCGAAGGACTAGGAGCCAAGCACTGTTAAGTTCTCAAACCGCACGCCCTGATGCTTTGATCCCACTTGGTTTGTCACCGTCTGCTAGAACAGTAACCTGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

417

Amino Acids

47.69

Weight (kDa)

9.54

Isoelectric Point (pI)

55.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 188
Acc36I ACCTGC 1 cut(s) 188
AccB1I GGYRCC 1 cut(s) 91
AccII CGCG 4 cut(s) 116, 179, 624, 953
AciI CCGC 6 cut(s) 11, 65, 285, 401, 951, 1188
AclWI GGATC 2 cut(s) 217, 1201
AcsI RAATTY 4 cut(s) 354, 481, 539, 938
AcuI CTGAAG 1 cut(s) 924
AcyI GRCGYC 1 cut(s) 92
AfaI GTAC 4 cut(s) 155, 627, 728, 1142
AfiI CCNNNNNNNGG 1 cut(s) 571
AflII CTTAAG 1 cut(s) 458
AgsI TTSAA 4 cut(s) 298, 353, 366, 470
AjnI CCWGG 2 cut(s) 39, 1244
AluBI AGCT 8 cut(s) 112, 436, 570, 616, 829, 892, 902, 971
AluI AGCT 8 cut(s) 112, 436, 570, 616, 829, 892, 902, 971
Alw26I GTCTC 4 cut(s) 271, 433, 558, 860
AlwI GGATC 2 cut(s) 217, 1201
AoxI GGCC 3 cut(s) 42, 184, 760
ApeKI GCWGC 5 cut(s) 77, 80, 383, 433, 743
ApoI RAATTY 4 cut(s) 354, 481, 539, 938
Asp700I GAANNNNTTC 1 cut(s) 498
AspLEI GCGC 2 cut(s) 55, 94
AspS9I GGNCC 2 cut(s) 761, 1091
AsuHPI GGTGA 5 cut(s) 212, 350, 490, 704, 1215
AvaII GGWCC 1 cut(s) 1091
BaeI ACNNNNGTAYC 2 cut(s) 657, 690
BanI GGYRCC 1 cut(s) 91
BarI GAAGNNNNNNTAC 2 cut(s) 1017, 1049
BbsI GAAGAC 1 cut(s) 1136
BbvI GCAGC 5 cut(s) 64, 92, 370, 445, 730
BccI CCATC 4 cut(s) 165, 256, 502, 790
BceAI ACGGC 1 cut(s) 78
BcgI CGANNNNNNTGC 2 cut(s) 69, 103
BciT130I CCWGG 2 cut(s) 41, 1246
BciVI GTATCC 1 cut(s) 316
BcoDI GTCTC 4 cut(s) 271, 433, 558, 860
BfaI CTAG 3 cut(s) 840, 1158, 1233
BfmI CTRYAG 2 cut(s) 78, 503
BfoI RGCGCY 1 cut(s) 95
BfrI CTTAAG 1 cut(s) 458
BfuAI ACCTGC 1 cut(s) 188
BfuI GTATCC 1 cut(s) 316
BglI GCCNNNNNGGC 1 cut(s) 50
BglII AGATCT 2 cut(s) 97, 411
BisI GCNGC 5 cut(s) 78, 81, 384, 434, 744
BlsI GCNGC 5 cut(s) 79, 82, 385, 435, 745
Bme1390I CCNGG 2 cut(s) 41, 1246
Bme18I GGWCC 1 cut(s) 1091
BmgT120I GGNCC 2 cut(s) 761, 1091
BmiI GGNNCC 3 cut(s) 93, 1092, 1163
BmrFI CCNGG 2 cut(s) 41, 1246
BmsI GCATC 2 cut(s) 826, 1189
BoxI GACNNNNGTC 1 cut(s) 122
BpiI GAAGAC 1 cut(s) 1136
BplI GAGNNNNNCTC 2 cut(s) 96, 128
BpmI CTGGAG 1 cut(s) 690
Bpu10I CCTNAGC 1 cut(s) 566
BsaAI YACGTR 1 cut(s) 730
BsaHI GRCGYC 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 29
Bsc4I CCNNNNNNNGG 1 cut(s) 571
Bse118I RCCGGY 1 cut(s) 986
Bse1I ACTGG 2 cut(s) 126, 673
BseBI CCWGG 2 cut(s) 41, 1246
BseDI CCNNGG 1 cut(s) 29
BseGI GGATG 2 cut(s) 543, 927
BseLI CCNNNNNNNGG 1 cut(s) 571
BseMII CTCAG 3 cut(s) 428, 825, 1040
BseNI ACTGG 2 cut(s) 126, 673
BseXI GCAGC 5 cut(s) 64, 92, 370, 445, 730
BsgI GTGCAG 1 cut(s) 312
Bsh1236I CGCG 4 cut(s) 116, 179, 624, 953
Bsh1285I CGRYCG 2 cut(s) 130, 520
BshFI GGCC 3 cut(s) 44, 186, 762
BshNI GGYRCC 1 cut(s) 91
BsiEI CGRYCG 2 cut(s) 130, 520
BsiSI CCGG 2 cut(s) 50, 987
BslFI GGGAC 1 cut(s) 1104
BslI CCNNNNNNNGG 1 cut(s) 571
BsmAI GTCTC 4 cut(s) 271, 433, 558, 860
BsmFI GGGAC 1 cut(s) 1104
BsnI GGCC 3 cut(s) 44, 186, 762
Bsp1407I TGTACA 1 cut(s) 1140
Bsp68I TCGCGA 1 cut(s) 116
BspACI CCGC 6 cut(s) 11, 65, 285, 401, 951, 1188
BspANI GGCC 3 cut(s) 44, 186, 762
BspCNI CTCAG 3 cut(s) 429, 824, 1041
BspFNI CGCG 4 cut(s) 116, 179, 624, 953
BspLI GGNNCC 3 cut(s) 93, 1092, 1163
BspMAI CTGCAG 2 cut(s) 82, 507
BspMI ACCTGC 1 cut(s) 188
BspPI GGATC 2 cut(s) 217, 1201
BspT107I GGYRCC 1 cut(s) 91
BspTI CTTAAG 1 cut(s) 458
BsrFI RCCGGY 1 cut(s) 986
BsrGI TGTACA 1 cut(s) 1140
BsrI ACTGG 2 cut(s) 126, 673
BssAI RCCGGY 1 cut(s) 986
BssECI CCNNGG 1 cut(s) 29
BssNI GRCGYC 1 cut(s) 92
Bst2UI CCWGG 2 cut(s) 41, 1246
Bst4CI ACNGT 6 cut(s) 333, 444, 610, 1173, 1227, 1240
Bst6I CTCTTC 2 cut(s) 858, 1020
BstACI GRCGYC 1 cut(s) 92
BstAFI CTTAAG 1 cut(s) 458
BstAUI TGTACA 1 cut(s) 1140
BstBAI YACGTR 1 cut(s) 730
BstC8I GCNNGC 4 cut(s) 114, 690, 992, 1192
BstDEI CTNAG 4 cut(s) 437, 566, 811, 1049
BstDSI CCRYGG 1 cut(s) 29
BstF5I GGATG 2 cut(s) 543, 927
BstFNI CGCG 4 cut(s) 116, 179, 624, 953
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 2 cut(s) 55, 94
BstMAI GTCTC 4 cut(s) 271, 433, 558, 860
BstMCI CGRYCG 2 cut(s) 130, 520
BstMWI GCNNNNNNNGC 6 cut(s) 50, 89, 389, 398, 430, 1072
BstNI CCWGG 2 cut(s) 41, 1246
BstPAI GACNNNNGTC 1 cut(s) 122
BstSCI CCNGG 2 cut(s) 39, 1244
BstSFI CTRYAG 2 cut(s) 78, 503
BstSNI TACGTA 1 cut(s) 730
BstUI CGCG 4 cut(s) 116, 179, 624, 953
BstV1I GCAGC 5 cut(s) 64, 92, 370, 445, 730
BstV2I GAAGAC 1 cut(s) 1136
BstX2I RGATCY 2 cut(s) 97, 411
BstYI RGATCY 2 cut(s) 97, 411
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 3 cut(s) 44, 186, 762
BtgI CCRYGG 1 cut(s) 29
BtsCI GGATG 2 cut(s) 543, 927
BtsIMutI CAGTG 3 cut(s) 119, 615, 1169
BtuMI TCGCGA 1 cut(s) 116
BveI ACCTGC 1 cut(s) 188
Cac8I GCNNGC 4 cut(s) 114, 690, 992, 1192
CfoI GCGC 2 cut(s) 55, 94
Cfr10I RCCGGY 1 cut(s) 986
Cfr13I GGNCC 2 cut(s) 761, 1091
Csp6I GTAC 4 cut(s) 154, 626, 727, 1141
CspCI CAANNNNNGTGG 2 cut(s) 1200, 1235
CviAII CATG 2 cut(s) 590, 758
CviQI GTAC 4 cut(s) 154, 626, 727, 1141
DdeI CTNAG 4 cut(s) 437, 566, 811, 1049
DinI GGCGCC 1 cut(s) 93
Eam1104I CTCTTC 2 cut(s) 858, 1020
EarI CTCTTC 2 cut(s) 858, 1020
EciI GGCGGA 2 cut(s) 80, 274
Eco105I TACGTA 1 cut(s) 730
Eco147I AGGCCT 1 cut(s) 44
Eco47I GGWCC 1 cut(s) 1091
Eco57I CTGAAG 1 cut(s) 924
EcoO109I RGGNCCY 1 cut(s) 1091
EcoRI GAATTC 2 cut(s) 481, 539
EcoRII CCWGG 2 cut(s) 39, 1244
EgeI GGCGCC 1 cut(s) 93
EheI GGCGCC 1 cut(s) 93
FaeI CATG 2 cut(s) 593, 761
FaqI GGGAC 1 cut(s) 1104
FatI CATG 2 cut(s) 589, 757
FauI CCCGC 3 cut(s) 18, 394, 958
FauNDI CATATG 2 cut(s) 318, 453
Fnu4HI GCNGC 5 cut(s) 78, 81, 384, 434, 744
FokI GGATG 2 cut(s) 530, 934
Fsp4HI GCNGC 5 cut(s) 78, 81, 384, 434, 744
FspBI CTAG 3 cut(s) 840, 1158, 1233
GlaI GCGC 2 cut(s) 54, 93
GluI GCNGC 5 cut(s) 78, 81, 384, 434, 744
GsuI CTGGAG 1 cut(s) 690
HaeII RGCGCY 1 cut(s) 95
HaeIII GGCC 3 cut(s) 44, 186, 762
HapII CCGG 2 cut(s) 50, 987
HhaI GCGC 2 cut(s) 55, 94
Hin1I GRCGYC 1 cut(s) 92
Hin1II CATG 2 cut(s) 593, 761
Hin6I GCGC 2 cut(s) 53, 92
HinP1I GCGC 2 cut(s) 53, 92
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HinfI GANTC 5 cut(s) 36, 212, 672, 955, 1081
HpaII CCGG 2 cut(s) 50, 987
HphI GGTGA 5 cut(s) 212, 350, 490, 704, 1215
Hpy166II GTNNAC 3 cut(s) 514, 577, 1141
Hpy188I TCNGA 8 cut(s) 104, 259, 345, 814, 918, 960, 1109, 1117
Hpy188III TCNNGA 3 cut(s) 115, 491, 547
Hpy8I GTNNAC 3 cut(s) 514, 577, 1141
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 4 cut(s) 266, 538, 629, 1146
HpyCH4III ACNGT 6 cut(s) 333, 444, 610, 1173, 1227, 1240
HpyCH4IV ACGT 2 cut(s) 254, 729
HpyCH4V TGCA 7 cut(s) 80, 329, 418, 505, 688, 772, 860
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 89, 389, 398, 430, 1072
HpyF3I CTNAG 4 cut(s) 437, 566, 811, 1049
HpySE526I ACGT 2 cut(s) 254, 729
Hsp92I GRCGYC 1 cut(s) 92
Hsp92II CATG 2 cut(s) 593, 761
HspAI GCGC 2 cut(s) 53, 92
KasI GGCGCC 1 cut(s) 91
LmnI GCTCC 2 cut(s) 621, 1161
Lsp1109I GCAGC 5 cut(s) 64, 92, 370, 445, 730
LweI GCATC 2 cut(s) 826, 1189
MaeI CTAG 3 cut(s) 840, 1158, 1233
MaeII ACGT 2 cut(s) 254, 729
MaeIII GTNAC 4 cut(s) 604, 710, 1221, 1240
MboII GAAGA 9 cut(s) 383, 506, 523, 875, 917, 935, 973, 1037, 1141
MflI RGATCY 2 cut(s) 97, 411
MluCI AATT 4 cut(s) 354, 481, 539, 938
Mly113I GGCGCC 1 cut(s) 92
MlyI GAGTC 3 cut(s) 681, 964, 1090
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 6 cut(s) 55, 61, 640, 912, 1000, 1242
MroXI GAANNNNTTC 1 cut(s) 498
MseI TTAA 3 cut(s) 459, 633, 1175
MspA1I CMGCKG 2 cut(s) 401, 436
MspCI CTTAAG 1 cut(s) 458
MspI CCGG 2 cut(s) 50, 987
MspR9I CCNGG 2 cut(s) 41, 1246
MvaI CCWGG 2 cut(s) 41, 1246
MvnI CGCG 4 cut(s) 116, 179, 624, 953
MwoI GCNNNNNNNGC 6 cut(s) 50, 89, 389, 398, 430, 1072
NarI GGCGCC 1 cut(s) 92
NdeI CATATG 2 cut(s) 318, 453
NlaIII CATG 2 cut(s) 593, 761
NlaIV GGNNCC 3 cut(s) 93, 1092, 1163
NmeAIII GCCGAG 1 cut(s) 1040
NmuCI GTSAC 3 cut(s) 604, 710, 1221
NruI TCGCGA 1 cut(s) 116
PaqCI CACCTGC 1 cut(s) 188
PceI AGGCCT 1 cut(s) 44
PcsI WCGNNNNNNNCGW 2 cut(s) 176, 189
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 2 cut(s) 36, 212
PkrI GCNGC 5 cut(s) 79, 82, 385, 435, 745
Ple19I CGATCG 2 cut(s) 130, 520
PleI GAGTC 3 cut(s) 680, 963, 1089
PluTI GGCGCC 1 cut(s) 95
PpsI GAGTC 3 cut(s) 680, 963, 1089
Ppu21I YACGTR 1 cut(s) 730
PpuMI RGGWCCY 1 cut(s) 1091
PshAI GACNNNNGTC 1 cut(s) 122
Psp5II RGGWCCY 1 cut(s) 1091
Psp6I CCWGG 2 cut(s) 39, 1244
PspGI CCWGG 2 cut(s) 39, 1244
PspN4I GGNNCC 3 cut(s) 93, 1092, 1163
PspPI GGNCC 2 cut(s) 761, 1091
PspPPI RGGWCCY 1 cut(s) 1091
PstI CTGCAG 2 cut(s) 82, 507
PsuI RGATCY 2 cut(s) 97, 411
PvuI CGATCG 2 cut(s) 130, 520
PvuII CAGCTG 1 cut(s) 436
RruI TCGCGA 1 cut(s) 116
RsaI GTAC 4 cut(s) 155, 627, 728, 1142
RsaNI GTAC 4 cut(s) 154, 626, 727, 1141
SaqAI TTAA 3 cut(s) 459, 633, 1175
SatI GCNGC 5 cut(s) 78, 81, 384, 434, 744
Sau96I GGNCC 2 cut(s) 761, 1091
SchI GAGTC 3 cut(s) 681, 964, 1090
ScrFI CCNGG 2 cut(s) 41, 1246
SfaNI GCATC 2 cut(s) 826, 1189
SfcI CTRYAG 2 cut(s) 78, 503
SfoI GGCGCC 1 cut(s) 93
SinI GGWCC 1 cut(s) 1091
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
SnaBI TACGTA 1 cut(s) 730
Sse9I AATT 4 cut(s) 354, 481, 539, 938
SseBI AGGCCT 1 cut(s) 44
SsiI CCGC 6 cut(s) 11, 65, 285, 401, 951, 1188
SspDI GGCGCC 1 cut(s) 91
SspMI CTAG 3 cut(s) 840, 1158, 1233
StuI AGGCCT 1 cut(s) 44
StyD4I CCNGG 2 cut(s) 39, 1244
TaaI ACNGT 6 cut(s) 333, 444, 610, 1173, 1227, 1240
TaiI ACGT 2 cut(s) 257, 732
TaqI TCGA 3 cut(s) 126, 192, 210
TasI AATT 4 cut(s) 354, 481, 539, 938
TatI WGTACW 1 cut(s) 1140
TfiI GAWTC 2 cut(s) 36, 212
Tru1I TTAA 3 cut(s) 459, 633, 1175
Tru9I TTAA 3 cut(s) 459, 633, 1175
TscAI CASTG 3 cut(s) 126, 615, 1176
TseFI GTSAC 3 cut(s) 604, 710, 1221
TseI GCWGC 5 cut(s) 77, 80, 383, 433, 743
Tsp45I GTSAC 3 cut(s) 604, 710, 1221
TspDTI ATGAA 4 cut(s) 248, 440, 532, 794
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 3 cut(s) 126, 615, 1176
Vha464I CTTAAG 1 cut(s) 458
VpaK11BI GGWCC 1 cut(s) 1091
XapI RAATTY 4 cut(s) 354, 481, 539, 938
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 3 cut(s) 840, 1158, 1233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.