RLG00000018903

protein import into mitochondrial matrix

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012425
Physical Location & Seq
Forward (+)
42523061 .. 42523635
575 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018903

Sequence Viewer

Length: 489 bp
ATGAAGGCCGAAGACGTCTTCGCATCTCTGGCAATTCCGTTGATGAAGCTCGTAGGTCTCAAGGCGATGGAGATGGCGACGAAGGGCCGGTTGACCTCCTTCATCATCCAAATAGATTCCGATTTAAGTTCTCAATATCAGAATTTCGGAGCCAGATCTGGACCATCGGGTCGGTCAGATCGGAACCAACAGTGTGCGAAGATAGAGATGTATGCCGCCAAAGCGGCAATGGCCTGCAATTTGGTAATTGAGGAGCAAAAAGATCAGCTAATGCAATTGGTTCATATCCTGAAGCAAATCGAGTCCCAAGTGAACAATCAGCAGAGCCACATGGTCGAAACCCTAGCCAATCGGCAGAGCCACATGGTCAAAACCCTAGCCAATCGCCGCCATTCCCTTTGCAAATTCTTCCAGAGAGCGATCGCCTACTTATCCACCATTGACCGCATCGACCACCAGGGCACCAGGGATTCGAGGTGGTGGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

18.46

Weight (kDa)

9.33

Isoelectric Point (pI)

58.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 168
AatII GACGTC 1 cut(s) 18
AccB1I GGYRCC 1 cut(s) 461
AciI CCGC 4 cut(s) 216, 224, 388, 445
AcsI RAATTY 2 cut(s) 142, 404
AcuI CTGAAG 1 cut(s) 311
AcyI GRCGYC 1 cut(s) 15
AjnI CCWGG 2 cut(s) 456, 464
AluBI AGCT 2 cut(s) 49, 268
AluI AGCT 2 cut(s) 49, 268
Alw26I GTCTC 1 cut(s) 62
AoxI GGCC 3 cut(s) 6, 85, 231
ApoI RAATTY 2 cut(s) 142, 404
AsiSI GCGATCGC 1 cut(s) 423
AspS9I GGNCC 2 cut(s) 85, 161
AvaII GGWCC 1 cut(s) 161
BaeGI GKGCMC 1 cut(s) 464
BanI GGYRCC 1 cut(s) 461
BbsI GAAGAC 2 cut(s) 10, 18
BccI CCATC 3 cut(s) 61, 67, 172
BciT130I CCWGG 2 cut(s) 458, 466
BcoDI GTCTC 1 cut(s) 62
BfaI CTAG 2 cut(s) 344, 377
BglI GCCNNNNNGGC 1 cut(s) 224
BglII AGATCT 1 cut(s) 155
BisI GCNGC 3 cut(s) 216, 225, 388
BlsI GCNGC 3 cut(s) 217, 226, 389
Bme1390I CCNGG 2 cut(s) 458, 466
Bme18I GGWCC 1 cut(s) 161
BmgT120I GGNCC 2 cut(s) 85, 161
BmiI GGNNCC 3 cut(s) 151, 185, 463
BmrFI CCNGG 2 cut(s) 458, 466
BmsI GCATC 2 cut(s) 32, 456
BpiI GAAGAC 2 cut(s) 10, 18
BpuEI CTTGAG 1 cut(s) 44
BsaHI GRCGYC 1 cut(s) 15
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 2 cut(s) 457, 465
Bse118I RCCGGY 1 cut(s) 87
Bse3DI GCAATG 1 cut(s) 234
BseBI CCWGG 2 cut(s) 458, 466
BseDI CCNNGG 2 cut(s) 457, 465
BseGI GGATG 1 cut(s) 105
BseMI GCAATG 1 cut(s) 234
BseRI GAGGAG 1 cut(s) 266
BseSI GKGCMC 1 cut(s) 464
Bsh1285I CGRYCG 1 cut(s) 423
BshFI GGCC 3 cut(s) 8, 87, 233
BshNI GGYRCC 1 cut(s) 461
BsiEI CGRYCG 1 cut(s) 423
BsiSI CCGG 1 cut(s) 88
BslFI GGGAC 1 cut(s) 289
BsmAI GTCTC 1 cut(s) 62
BsmFI GGGAC 1 cut(s) 289
BsnI GGCC 3 cut(s) 8, 87, 233
Bso31I GGTCTC 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 464
Bsp143I GATC 4 cut(s) 155, 178, 262, 420
BspACI CCGC 4 cut(s) 216, 224, 388, 445
BspANI GGCC 3 cut(s) 8, 87, 233
BspLI GGNNCC 3 cut(s) 151, 185, 463
BspT107I GGYRCC 1 cut(s) 461
BspTNI GGTCTC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 234
BsrFI RCCGGY 1 cut(s) 87
BssAI RCCGGY 1 cut(s) 87
BssECI CCNNGG 2 cut(s) 457, 465
BssMI GATC 4 cut(s) 155, 178, 262, 420
BssNI GRCGYC 1 cut(s) 15
Bst2UI CCWGG 2 cut(s) 458, 466
Bst4CI ACNGT 1 cut(s) 192
BstACI GRCGYC 1 cut(s) 15
BstC8I GCNNGC 1 cut(s) 235
BstF5I GGATG 1 cut(s) 105
BstKTI GATC 4 cut(s) 158, 181, 265, 423
BstMAI GTCTC 1 cut(s) 62
BstMBI GATC 4 cut(s) 155, 178, 262, 420
BstMCI CGRYCG 1 cut(s) 423
BstMWI GCNNNNNNNGC 4 cut(s) 29, 221, 224, 230
BstNI CCWGG 2 cut(s) 458, 466
BstSCI CCNGG 2 cut(s) 456, 464
BstSLI GKGCMC 1 cut(s) 464
BstV2I GAAGAC 2 cut(s) 10, 18
BstX2I RGATCY 1 cut(s) 155
BstYI RGATCY 1 cut(s) 155
BsuRI GGCC 3 cut(s) 8, 87, 233
BtgZI GCGATG 1 cut(s) 80
BtsCI GGATG 1 cut(s) 105
BtsIMutI CAGTG 1 cut(s) 197
Cac8I GCNNGC 1 cut(s) 235
Cfr10I RCCGGY 1 cut(s) 87
Cfr13I GGNCC 2 cut(s) 85, 161
CviAII CATG 2 cut(s) 331, 364
DpnI GATC 4 cut(s) 157, 180, 264, 422
DpnII GATC 4 cut(s) 155, 178, 262, 420
DrdI GACNNNNNNGTC 1 cut(s) 168
DseDI GACNNNNNNGTC 1 cut(s) 168
Eco31I GGTCTC 1 cut(s) 62
Eco47I GGWCC 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 311
EcoRII CCWGG 2 cut(s) 456, 464
FaeI CATG 2 cut(s) 334, 367
FaiI YATR 4 cut(s) 213, 285, 332, 365
FaqI GGGAC 1 cut(s) 289
FatI CATG 2 cut(s) 330, 363
Fnu4HI GCNGC 3 cut(s) 216, 225, 388
FokI GGATG 1 cut(s) 92
Fsp4HI GCNGC 3 cut(s) 216, 225, 388
FspBI CTAG 2 cut(s) 344, 377
GluI GCNGC 3 cut(s) 216, 225, 388
HaeIII GGCC 3 cut(s) 8, 87, 233
HapII CCGG 1 cut(s) 88
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 2 cut(s) 334, 367
HincII GTYRAC 1 cut(s) 93
HindII GTYRAC 1 cut(s) 93
HinfI GANTC 3 cut(s) 116, 302, 470
HpaII CCGG 1 cut(s) 88
Hpy166II GTNNAC 2 cut(s) 93, 313
Hpy188I TCNGA 5 cut(s) 121, 141, 149, 178, 183
Hpy188III TCNNGA 3 cut(s) 159, 289, 412
Hpy8I GTNNAC 2 cut(s) 93, 313
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 2 cut(s) 76, 109
HpyCH4III ACNGT 1 cut(s) 192
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 3 cut(s) 237, 274, 402
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 221, 224, 230
HpySE526I ACGT 1 cut(s) 15
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 2 cut(s) 334, 367
Kzo9I GATC 4 cut(s) 155, 178, 262, 420
LmnI GCTCC 2 cut(s) 149, 253
LweI GCATC 2 cut(s) 32, 456
MaeI CTAG 2 cut(s) 344, 377
MaeII ACGT 1 cut(s) 15
MalI GATC 4 cut(s) 157, 180, 264, 422
MboI GATC 4 cut(s) 155, 178, 262, 420
MboII GAAGA 4 cut(s) 10, 23, 211, 400
MfeI CAATTG 1 cut(s) 275
MflI RGATCY 1 cut(s) 155
MhlI GDGCHC 1 cut(s) 464
MluCI AATT 6 cut(s) 33, 142, 238, 246, 275, 404
MlyI GAGTC 1 cut(s) 311
MnlI CCTC 3 cut(s) 106, 244, 468
MseI TTAA 1 cut(s) 125
MspI CCGG 1 cut(s) 88
MspR9I CCNGG 2 cut(s) 458, 466
MunI CAATTG 1 cut(s) 275
MvaI CCWGG 2 cut(s) 458, 466
MwoI GCNNNNNNNGC 4 cut(s) 29, 221, 224, 230
NdeII GATC 4 cut(s) 155, 178, 262, 420
NlaIII CATG 2 cut(s) 334, 367
NlaIV GGNNCC 3 cut(s) 151, 185, 463
PfeI GAWTC 2 cut(s) 116, 470
PkrI GCNGC 3 cut(s) 217, 226, 389
Ple19I CGATCG 1 cut(s) 423
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
Psp6I CCWGG 2 cut(s) 456, 464
PspGI CCWGG 2 cut(s) 456, 464
PspN4I GGNNCC 3 cut(s) 151, 185, 463
PspPI GGNCC 2 cut(s) 85, 161
PsuI RGATCY 1 cut(s) 155
PvuI CGATCG 1 cut(s) 423
RgaI GCGATCGC 1 cut(s) 423
SaqAI TTAA 1 cut(s) 125
SatI GCNGC 3 cut(s) 216, 225, 388
Sau3AI GATC 4 cut(s) 155, 178, 262, 420
Sau96I GGNCC 2 cut(s) 85, 161
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 2 cut(s) 458, 466
SduI GDGCHC 1 cut(s) 464
SetI ASST 6 cut(s) 18, 51, 58, 98, 270, 479
SfaAI GCGATCGC 1 cut(s) 423
SfaNI GCATC 2 cut(s) 32, 456
SgfI GCGATCGC 1 cut(s) 423
SinI GGWCC 1 cut(s) 161
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 6 cut(s) 33, 142, 238, 246, 275, 404
SsiI CCGC 4 cut(s) 216, 224, 388, 445
SspMI CTAG 2 cut(s) 344, 377
StyD4I CCNGG 2 cut(s) 456, 464
TaaI ACNGT 1 cut(s) 192
TaiI ACGT 1 cut(s) 18
TaqI TCGA 4 cut(s) 300, 336, 450, 473
TaqII GACCGA 1 cut(s) 162
TasI AATT 6 cut(s) 33, 142, 238, 246, 275, 404
TauI GCSGC 3 cut(s) 218, 227, 390
TfiI GAWTC 2 cut(s) 116, 470
Tru1I TTAA 1 cut(s) 125
Tru9I TTAA 1 cut(s) 125
TscAI CASTG 1 cut(s) 197
TspDTI ATGAA 4 cut(s) 17, 59, 91, 272
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 1 cut(s) 197
VpaK11BI GGWCC 1 cut(s) 161
XapI RAATTY 2 cut(s) 142, 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 226
XspI CTAG 2 cut(s) 344, 377
ZraI GACGTC 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.