AT5G63500

Protein of unknown function (DUF3339)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0019129
Physical Location & Seq
Forward (+)
25423370 .. 25423880
511 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G63500.1

Sequence Viewer

Length: 210 bp
ATGGCGGATTGGGGCCCAGTGTTGATCGCCGTGATCCTCTTCGTAGTTCTATCGCCGGGGCTTCTTTTCCAGATTCCGGCGGGAGGTCGAGTTGTCGAATTCGGTAATATGCAGACGAGTGGCGCTTCGATTCTCGTTCACGCCATCATTTTCTTTGGTCTCATCACTATCTTTACCATCGCCATAAACGTCCACATCTATTCCGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3339

Protein Analysis

69

Amino Acids

7.33

Weight (kDa)

5.95

Isoelectric Point (pI)

30.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3339 PF11820 1 - 67 3.6e-35 Protein of unknown function (DUF3339)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0019129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48660 AT5G63500
malus_domestica MD06G1140200.v1.1
prunus_persica Prupe.5G149500_v2.0.a1
pyrus_communis pycom06g13050 pycom14g12930
rosa_rugosa Rorug06G0500800
rosa_wichuraiana Rw7G009180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 80
AclWI GGATC 1 cut(s) 28
AcsI RAATTY 1 cut(s) 98
AfiI CCNNNNNNNGG 2 cut(s) 76, 83
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 1 cut(s) 28
AoxI GGCC 1 cut(s) 13
ApaI GGGCCC 1 cut(s) 17
ApoI RAATTY 1 cut(s) 98
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 2 cut(s) 13, 14
AsuC2I CCSGG 1 cut(s) 57
BaeGI GKGCMC 1 cut(s) 17
BanII GRGCYC 1 cut(s) 17
BccI CCATC 2 cut(s) 152, 185
BceAI ACGGC 1 cut(s) 14
BcnI CCSGG 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 164
BfoI RGCGCY 1 cut(s) 126
Bme1390I CCNGG 1 cut(s) 57
BmgT120I GGNCC 2 cut(s) 13, 14
BmiI GGNNCC 2 cut(s) 14, 15
BmrFI CCNGG 1 cut(s) 57
BmrI ACTGGG 1 cut(s) 11
BmuI ACTGGG 1 cut(s) 11
BpuMI CCSGG 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 56
BsaWI WCCGGW 1 cut(s) 203
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 83
Bse1I ACTGG 1 cut(s) 17
BseDI CCNNGG 1 cut(s) 56
BseLI CCNNNNNNNGG 2 cut(s) 76, 83
BseNI ACTGG 1 cut(s) 17
BseSI GKGCMC 1 cut(s) 17
BshFI GGCC 1 cut(s) 15
BsiSI CCGG 3 cut(s) 56, 77, 204
BslI CCNNNNNNNGG 2 cut(s) 76, 83
BsmAI GTCTC 1 cut(s) 164
BsnI GGCC 1 cut(s) 15
Bso31I GGTCTC 1 cut(s) 164
Bsp120I GGGCCC 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 2 cut(s) 24, 33
BspACI CCGC 2 cut(s) 5, 80
BspANI GGCC 1 cut(s) 15
BspLI GGNNCC 2 cut(s) 14, 15
BspPI GGATC 1 cut(s) 28
BspTNI GGTCTC 1 cut(s) 164
BsrI ACTGG 1 cut(s) 17
BssECI CCNNGG 1 cut(s) 56
BssMI GATC 2 cut(s) 24, 33
Bst6I CTCTTC 1 cut(s) 44
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 2 cut(s) 27, 36
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 2 cut(s) 24, 33
BstSCI CCNGG 1 cut(s) 55
BstSLI GKGCMC 1 cut(s) 17
BsuRI GGCC 1 cut(s) 15
BtgZI GCGATG 1 cut(s) 163
BtsIMutI CAGTG 1 cut(s) 24
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 2 cut(s) 13, 14
CviJI RGCY 2 cut(s) 15, 61
CviKI_1 RGCY 2 cut(s) 15, 61
DpnI GATC 2 cut(s) 26, 35
DpnII GATC 2 cut(s) 24, 33
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 17
Eco31I GGTCTC 1 cut(s) 164
EcoO109I RGGNCCY 1 cut(s) 13
EcoRI GAATTC 1 cut(s) 98
EcoT38I GRGCYC 1 cut(s) 17
FaiI YATR 2 cut(s) 110, 185
FauI CCCGC 1 cut(s) 73
FriOI GRGCYC 1 cut(s) 17
GlaI GCGC 1 cut(s) 124
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 1 cut(s) 15
HapII CCGG 3 cut(s) 56, 77, 204
HhaI GCGC 1 cut(s) 125
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HinfI GANTC 2 cut(s) 73, 130
HpaII CCGG 3 cut(s) 56, 77, 204
Hpy166II GTNNAC 2 cut(s) 139, 193
Hpy188III TCNNGA 1 cut(s) 70
Hpy8I GTNNAC 2 cut(s) 139, 193
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 1 cut(s) 112
HpySE526I ACGT 1 cut(s) 189
HspAI GCGC 1 cut(s) 123
Kzo9I GATC 2 cut(s) 24, 33
LpnPI CCDG 4 cut(s) 30, 69, 83, 90
MaeII ACGT 1 cut(s) 189
MalI GATC 2 cut(s) 26, 35
MboI GATC 2 cut(s) 24, 33
MboII GAAGA 1 cut(s) 31
MhlI GDGCHC 1 cut(s) 17
MluCI AATT 1 cut(s) 98
MnlI CCTC 2 cut(s) 47, 77
MseI TTAA 1 cut(s) 208
MspI CCGG 3 cut(s) 56, 77, 204
MspR9I CCNGG 1 cut(s) 57
NciI CCSGG 1 cut(s) 57
NdeII GATC 2 cut(s) 24, 33
NlaIV GGNNCC 2 cut(s) 14, 15
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PfeI GAWTC 2 cut(s) 73, 130
PspN4I GGNNCC 2 cut(s) 14, 15
PspOMI GGGCCC 1 cut(s) 13
PspPI GGNCC 2 cut(s) 13, 14
SaqAI TTAA 1 cut(s) 208
Sau3AI GATC 2 cut(s) 24, 33
Sau96I GGNCC 2 cut(s) 13, 14
ScrFI CCNGG 1 cut(s) 57
SduI GDGCHC 1 cut(s) 17
SetI ASST 2 cut(s) 88, 192
Sse9I AATT 1 cut(s) 98
SsiI CCGC 2 cut(s) 5, 80
StyD4I CCNGG 1 cut(s) 55
TaiI ACGT 1 cut(s) 192
TaqI TCGA 3 cut(s) 88, 96, 128
TasI AATT 1 cut(s) 98
TfiI GAWTC 2 cut(s) 73, 130
Tru1I TTAA 1 cut(s) 208
Tru9I TTAA 1 cut(s) 208
TscAI CASTG 1 cut(s) 24
TspRI CASTG 1 cut(s) 24
XapI RAATTY 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.