pycom06g13050

Protein of unknown function (DUF3339)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0019129
Physical Location & Seq
Reverse (-)
18123142 .. 18123740
599 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g13050.1

Sequence Viewer

Length: 378 bp
ATGCAGTGCACTTGTCCGACAATTTATGTTCCCAACCTCCTATCTCTTCACAAACTTCCAAAACCCATCCACTTTCCTCTCAGATCTTCCAAACCCGTCTATAACTTTCTCTTCCTCCTCCTCCTCCTCCTCGCTGATCTGGTTTTGAAGAGAAGAGCAGGTGCAAAAATGGCAGACTGGGGTCCGGTGGTGATAGCGGTGGTGCTGTTTGTGCTGTTGACACCCGGGCTTTTGTTTCAGATACCCGGACGTGGGAGGTTGGTGGAGTTCGGGAACATGCAGACCAGCGCCGCTTCCATCGTGGTGCACGCCATCATCTACTTTGGCCTCCTCACCATCTTCCTTATTGCCATTGGAGTTCACATCTATGCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3339

Protein Analysis

126

Amino Acids

13.77

Weight (kDa)

9.94

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3339 PF11820 57 - 123 1e-35 Protein of unknown function (DUF3339)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48660 AT5G63500
malus_domestica MD06G1140200.v1.1
prunus_persica Prupe.5G149500_v2.0.a1
pyrus_communis pycom06g13050 pycom14g12930
rosa_rugosa Rorug06G0500800
rosa_wichuraiana Rw7G009180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 149
Acc36I ACCTGC 1 cut(s) 149
AciI CCGC 2 cut(s) 197, 291
AfiI CCNNNNNNNGG 2 cut(s) 251, 252
AgsI TTSAA 1 cut(s) 148
AjiI CACGTC 1 cut(s) 251
Alw21I GWGCWC 2 cut(s) 11, 309
Alw44I GTGCAC 2 cut(s) 7, 305
Ama87I CYCGRG 1 cut(s) 224
AoxI GGCC 1 cut(s) 325
ApaLI GTGCAC 2 cut(s) 7, 305
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 182
AsuC2I CCSGG 3 cut(s) 225, 226, 246
AsuHPI GGTGA 2 cut(s) 202, 325
AvaI CYCGRG 1 cut(s) 224
AvaII GGWCC 1 cut(s) 182
BaeGI GKGCMC 2 cut(s) 11, 309
Bbv12I GWGCWC 2 cut(s) 11, 309
BccI CCATC 4 cut(s) 74, 305, 320, 344
BcnI CCSGG 3 cut(s) 225, 226, 246
BfoI RGCGCY 1 cut(s) 291
BfuAI ACCTGC 1 cut(s) 149
BglII AGATCT 1 cut(s) 83
BisI GCNGC 1 cut(s) 291
BlsI GCNGC 1 cut(s) 292
Bme1390I CCNGG 3 cut(s) 225, 226, 246
Bme18I GGWCC 1 cut(s) 182
BmeT110I CYCGRG 1 cut(s) 224
BmgBI CACGTC 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 182
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 3 cut(s) 225, 226, 246
BmrI ACTGGG 1 cut(s) 187
BmuI ACTGGG 1 cut(s) 187
BoxI GACNNNNGTC 1 cut(s) 180
BpuMI CCSGG 3 cut(s) 225, 226, 246
BsaJI CCNNGG 1 cut(s) 224
BsaWI WCCGGW 1 cut(s) 184
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 252
Bse1I ACTGG 1 cut(s) 182
BseDI CCNNGG 1 cut(s) 224
BseGI GGATG 1 cut(s) 66
BseLI CCNNNNNNNGG 2 cut(s) 251, 252
BseMII CTCAG 1 cut(s) 94
BseNI ACTGG 1 cut(s) 182
BseRI GAGGAG 6 cut(s) 107, 110, 113, 116, 119, 320
BseSI GKGCMC 2 cut(s) 11, 309
BshFI GGCC 1 cut(s) 327
BsiHKAI GWGCWC 2 cut(s) 11, 309
BsiHKCI CYCGRG 1 cut(s) 224
BsiSI CCGG 3 cut(s) 185, 225, 246
BslI CCNNNNNNNGG 2 cut(s) 251, 252
BsnI GGCC 1 cut(s) 327
BsoBI CYCGRG 1 cut(s) 224
Bsp1286I GDGCHC 2 cut(s) 11, 309
Bsp143I GATC 2 cut(s) 83, 136
BspACI CCGC 2 cut(s) 197, 291
BspANI GGCC 1 cut(s) 327
BspCNI CTCAG 1 cut(s) 93
BspLI GGNNCC 1 cut(s) 183
BspMI ACCTGC 1 cut(s) 149
BspQI GCTCTTC 1 cut(s) 148
BsrI ACTGG 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 224
BssMI GATC 2 cut(s) 83, 136
Bst6I CTCTTC 4 cut(s) 51, 116, 143, 148
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 1 cut(s) 80
BstF5I GGATG 1 cut(s) 66
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 2 cut(s) 86, 139
BstMBI GATC 2 cut(s) 83, 136
BstMWI GCNNNNNNNGC 2 cut(s) 170, 211
BstNSI RCATGY 1 cut(s) 280
BstPAI GACNNNNGTC 1 cut(s) 180
BstSCI CCNGG 3 cut(s) 223, 224, 244
BstSLI GKGCMC 2 cut(s) 11, 309
BstX2I RGATCY 1 cut(s) 83
BstYI RGATCY 1 cut(s) 83
BsuRI GGCC 1 cut(s) 327
BtrI CACGTC 1 cut(s) 251
BtsCI GGATG 1 cut(s) 66
BtsI GCAGTG 1 cut(s) 11
BtsIMutI CAGTG 1 cut(s) 11
BveI ACCTGC 1 cut(s) 149
Cac8I GCNNGC 1 cut(s) 309
CfoI GCGC 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 182
Cfr9I CCCGGG 1 cut(s) 224
CviAII CATG 1 cut(s) 277
CviJI RGCY 2 cut(s) 229, 327
CviKI_1 RGCY 2 cut(s) 229, 327
DdeI CTNAG 1 cut(s) 80
DpnI GATC 2 cut(s) 85, 138
DpnII GATC 2 cut(s) 83, 136
Eam1104I CTCTTC 4 cut(s) 51, 116, 143, 148
EarI CTCTTC 4 cut(s) 51, 116, 143, 148
Eco47I GGWCC 1 cut(s) 182
Eco88I CYCGRG 1 cut(s) 224
FaeI CATG 1 cut(s) 280
FaiI YATR 4 cut(s) 27, 102, 278, 369
FatI CATG 1 cut(s) 276
Fnu4HI GCNGC 1 cut(s) 291
FokI GGATG 1 cut(s) 53
Fsp4HI GCNGC 1 cut(s) 291
GlaI GCGC 1 cut(s) 289
GluI GCNGC 1 cut(s) 291
HaeII RGCGCY 1 cut(s) 291
HaeIII GGCC 1 cut(s) 327
HapII CCGG 3 cut(s) 185, 225, 246
HhaI GCGC 1 cut(s) 290
Hin1II CATG 1 cut(s) 280
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HincII GTYRAC 1 cut(s) 219
HindII GTYRAC 1 cut(s) 219
HpaII CCGG 3 cut(s) 185, 225, 246
HphI GGTGA 2 cut(s) 202, 325
Hpy166II GTNNAC 4 cut(s) 9, 219, 307, 361
Hpy188I TCNGA 3 cut(s) 18, 83, 240
Hpy188III TCNNGA 1 cut(s) 271
Hpy8I GTNNAC 4 cut(s) 9, 219, 307, 361
HpyCH4IV ACGT 1 cut(s) 250
HpyCH4V TGCA 5 cut(s) 4, 9, 164, 280, 307
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 211
HpyF3I CTNAG 1 cut(s) 80
HpySE526I ACGT 1 cut(s) 250
Hsp92II CATG 1 cut(s) 280
HspAI GCGC 1 cut(s) 288
Kzo9I GATC 2 cut(s) 83, 136
LguI GCTCTTC 1 cut(s) 148
LpnPI CCDG 8 cut(s) 125, 144, 163, 198, 238, 259, 298, 357
MaeII ACGT 1 cut(s) 250
MalI GATC 2 cut(s) 85, 138
MboI GATC 2 cut(s) 83, 136
MboII GAAGA 6 cut(s) 38, 78, 103, 160, 165, 331
MflI RGATCY 1 cut(s) 83
MhlI GDGCHC 2 cut(s) 11, 309
MluCI AATT 1 cut(s) 21
MmeI TCCRAC 1 cut(s) 41
MseI TTAA 1 cut(s) 376
MslI CAYNNNNRTG 2 cut(s) 302, 366
MspI CCGG 3 cut(s) 185, 225, 246
MspR9I CCNGG 3 cut(s) 225, 226, 246
MwoI GCNNNNNNNGC 2 cut(s) 170, 211
NciI CCSGG 3 cut(s) 225, 226, 246
NdeII GATC 2 cut(s) 83, 136
NlaIII CATG 1 cut(s) 280
NlaIV GGNNCC 1 cut(s) 183
NspI RCATGY 1 cut(s) 280
PaqCI CACCTGC 1 cut(s) 149
PciSI GCTCTTC 1 cut(s) 148
PkrI GCNGC 1 cut(s) 292
PshAI GACNNNNGTC 1 cut(s) 180
PspN4I GGNNCC 1 cut(s) 183
PspPI GGNCC 1 cut(s) 182
PsuI RGATCY 1 cut(s) 83
RseI CAYNNNNRTG 2 cut(s) 302, 366
SapI GCTCTTC 1 cut(s) 148
SaqAI TTAA 1 cut(s) 376
SatI GCNGC 1 cut(s) 291
Sau3AI GATC 2 cut(s) 83, 136
Sau96I GGNCC 1 cut(s) 182
ScrFI CCNGG 3 cut(s) 225, 226, 246
SduI GDGCHC 2 cut(s) 11, 309
SetI ASST 4 cut(s) 39, 163, 253, 260
SinI GGWCC 1 cut(s) 182
SmaI CCCGGG 1 cut(s) 226
SmiMI CAYNNNNRTG 2 cut(s) 302, 366
Sse9I AATT 1 cut(s) 21
SsiI CCGC 2 cut(s) 197, 291
StyD4I CCNGG 3 cut(s) 223, 224, 244
TaiI ACGT 1 cut(s) 253
TasI AATT 1 cut(s) 21
TauI GCSGC 1 cut(s) 293
Tru1I TTAA 1 cut(s) 376
Tru9I TTAA 1 cut(s) 376
TspMI CCCGGG 1 cut(s) 224
VneI GTGCAC 2 cut(s) 7, 305
VpaK11BI GGWCC 1 cut(s) 182
XceI RCATGY 1 cut(s) 280
XmaI CCCGGG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.