AT5G63850

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014916
Physical Location & Seq
Forward (+)
25550937 .. 25553656
2720 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G63850.1

Sequence Viewer

Length: 1401 bp
ATGGATGTTCCACGGCCAGCTTTCAAATGTTTTGATGACGATGGCCGGCTTAAACGTTCAGGGACGGTTTGGACCGCGAGTGCGCATATCATAACCGCCGTGATTGGATCTGGTGTTCTATCGCTTGCGTGGGCTATAGGTCAACTCGGTTGGATCGCAGGTCCTACAGTGATGTTGTTGTTCTCTTTTGTCACTTACTACTCTTCCACGCTTCTTAGCGACTGCTACAGAACCGGAGATCCTGTCTCTGGGAAGAGAAACTATACTTACATGGACGCTGTCCGATCAATCCTAGGTGGCTTTAGGTTCAAGATTTGTGGGCTGATTCAGTATTTGAATCTGTTTGGTATCACGGTCGGGTACACAATCGCAGCATCTATAAGTATGATGGCGATCAAGAGGTCCAACTGTTTCCACGAGAGCGGAGGGAAAAACCCGTGTCACATGTCGAGCAATCCATACATGATCATGTTTGGTGTGACCGAGATCTTGCTCTCTCAGATCAAAGATTTTGACCAGATTTGGTGGCTCTCCATTGTCGCTGCTATCATGTCCTTCACATACTCTGCAATCGGTTTAGCTCTCGGAATCATTCAAGTCGCGGCAAATGGAGTTGTCAAGGGAAGTCTCACCGGAATTAGCATCGGCGCAGTGACTCAGACCCAAAAAATATGGAGAACCTTTCAAGCACTTGGAGACATTGCCTTTGCTTATTCATACTCTGTTGTTCTTATTGAAATTCAGGACACTGTAAGATCTCCACCAGCAGAATCAAAAACGATGAAGATCGCCACAAGAATCAGCATCGCTGTTACAACGACATTTTACATGCTATGTGGTTGTATGGGCTATGCCGCCTTCGGAGATAAAGCACCGGGAAACCTCTTAACCGGTTTTGGTTTCTACAATCCGTTTTGGCTCCTTGACGTGGCTAACGCTGCCATAGTTATCCACCTTGTAGGAGCTTATCAAGTCTTTGCTCAGCCCATCTTCGCCTTTATTGAGAAACAAGCTGCCGCTAGGTTTCCCGACAGTGACTTGGTGACCAAGGAATACGAAATCCGAATCCCTGGTTTTAGGTCACCGTACAAAGTCAACGTTTTCAGAGCAGTTTACCGAAGCGGGTTTGTGGTTTTGACCACTGTGATATCCATGCTTATGCCGTTTTTCAACGACGTCGTAGGGATTTTAGGTGCGTTAGGGTTTTGGCCTTTGACGGTTTACTTTCCGGTGGAGATGTATATAAGACAGAGGAAGGTTGAGAGATGGAGTATGAAGTGGGTTTGTCTGCAGATGTTGAGCTGTGGTTGTTTGATGATCACGTTGGTCGCCGGAGTTGGCTCCATCGCCGGAGTAATGCTAGACCTTAAGGTTTACAAGCCGTTCAAGACTACTTACTAA

Protein Analysis

466

Amino Acids

51.43

Weight (kDa)

9.21

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 20 - 453 3.2e-123 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09220 AT5G63850
fragaria_vesca FvH4_2g01330
malus_domestica MD05G1120400.v1.1
prunus_persica Prupe.4G277700_v2.0.a1 Prupe.8G164200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0252251
rosa_laevigata RLG00000015401
rosa_multiflora Rmu_sc0006252.1_g000018 Rmu_sc0009799.1_g000021
rosa_roxburghii Rroxscaffold_7G00216210
rosa_rugosa Rorug05G0506400
rosa_samantha Rh6AG020200 Rh6BG015800 Rh6CG013000
rosa_wichuraiana Rw6G001660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1179
Acc16I TGCGCA 1 cut(s) 84
Acc36I ACCTGC 1 cut(s) 149
AccBSI CCGCTC 1 cut(s) 423
AccII CGCG 2 cut(s) 77, 602
AciI CCGC 7 cut(s) 75, 96, 423, 602, 855, 1017, 1122
AclI AACGTT 2 cut(s) 55, 1098
AclWI GGATC 3 cut(s) 115, 161, 233
AcoI YGGCCR 2 cut(s) 14, 43
AcsI RAATTY 1 cut(s) 738
AcyI GRCGYC 1 cut(s) 1176
AfaI GTAC 2 cut(s) 362, 1088
AfiI CCNNNNNNNGG 2 cut(s) 248, 928
AflII CTTAAG 1 cut(s) 1367
AflIII ACRYGT 1 cut(s) 444
AgeI ACCGGT 1 cut(s) 890
AgsI TTSAA 8 cut(s) 25, 310, 337, 596, 686, 737, 1171, 1387
AjiI CACGTC 1 cut(s) 928
AjnI CCWGG 1 cut(s) 1069
AloI GAACNNNNNNTCC 4 cut(s) 99, 131, 223, 255
AluBI AGCT 5 cut(s) 20, 581, 965, 1013, 1302
AluI AGCT 5 cut(s) 20, 581, 965, 1013, 1302
Alw26I GTCTC 3 cut(s) 250, 632, 690
AlwI GGATC 3 cut(s) 115, 161, 233
AoxI GGCC 3 cut(s) 14, 43, 1208
ApeKI GCWGC 4 cut(s) 371, 542, 938, 1013
ApoI RAATTY 1 cut(s) 738
ArsI GACNNNNNNTTYG 2 cut(s) 689, 721
AsiGI ACCGGT 1 cut(s) 890
AspA2I CCTAGG 1 cut(s) 292
AspLEI GCGC 2 cut(s) 85, 650
AspS9I GGNCC 3 cut(s) 72, 161, 402
AsuC2I CCSGG 1 cut(s) 876
AsuHPI GGTGA 3 cut(s) 622, 1054, 1074
AvaII GGWCC 3 cut(s) 72, 161, 402
AvrII CCTAGG 1 cut(s) 292
BauI CACGAG 1 cut(s) 416
BbvI GCAGC 4 cut(s) 383, 529, 925, 1000
BccI CCATC 5 cut(s) 35, 382, 995, 1260, 1352
BceAI ACGGC 4 cut(s) 29, 83, 1147, 1366
BciT130I CCWGG 1 cut(s) 1071
BclI TGATCA 2 cut(s) 465, 1317
BcnI CCSGG 1 cut(s) 876
BcoDI GTCTC 3 cut(s) 250, 632, 690
BfaI CTAG 3 cut(s) 293, 1020, 1361
BfmI CTRYAG 4 cut(s) 135, 165, 226, 1289
BfrI CTTAAG 1 cut(s) 1367
BfuAI ACCTGC 1 cut(s) 149
BglII AGATCT 2 cut(s) 486, 755
BisI GCNGC 7 cut(s) 372, 543, 603, 855, 939, 1014, 1017
BlnI CCTAGG 1 cut(s) 292
BlpI GCTNAGC 1 cut(s) 981
BlsI GCNGC 7 cut(s) 373, 544, 604, 856, 940, 1015, 1018
Bme1390I CCNGG 2 cut(s) 876, 1071
Bme18I GGWCC 3 cut(s) 72, 161, 402
BmgBI CACGTC 1 cut(s) 928
BmgT120I GGNCC 3 cut(s) 72, 161, 402
BmiI GGNNCC 2 cut(s) 920, 1342
BmrFI CCNGG 2 cut(s) 876, 1071
BmsI GCATC 3 cut(s) 383, 651, 813
Bpu1102I GCTNAGC 1 cut(s) 981
BpuMI CCSGG 1 cut(s) 876
BsaBI GATNNNNATC 2 cut(s) 392, 785
BsaHI GRCGYC 1 cut(s) 1176
BsaJI CCNNGG 4 cut(s) 11, 292, 1047, 1069
BsaWI WCCGGW 4 cut(s) 233, 632, 890, 1228
BsaXI ACNNNNNCTCC 2 cut(s) 228, 258
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 928
Bse118I RCCGGY 2 cut(s) 45, 890
Bse3DI GCAATG 1 cut(s) 699
Bse8I GATNNNNATC 2 cut(s) 392, 785
BseBI CCWGG 1 cut(s) 1071
BseDI CCNNGG 4 cut(s) 11, 292, 1047, 1069
BseGI GGATG 1 cut(s) 10
BseJI GATNNNNATC 2 cut(s) 392, 785
BseLI CCNNNNNNNGG 2 cut(s) 248, 928
BseMI GCAATG 1 cut(s) 699
BseMII CTCAG 3 cut(s) 512, 671, 995
BseXI GCAGC 4 cut(s) 383, 529, 925, 1000
Bsh1236I CGCG 2 cut(s) 77, 602
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 3 cut(s) 16, 45, 1210
BshTI ACCGGT 1 cut(s) 890
BsiEI CGRYCG 1 cut(s) 357
BsiSI CCGG 8 cut(s) 46, 234, 633, 875, 891, 1229, 1332, 1350
BslFI GGGAC 1 cut(s) 76
BslI CCNNNNNNNGG 2 cut(s) 248, 928
BsmAI GTCTC 3 cut(s) 250, 632, 690
BsmFI GGGAC 1 cut(s) 76
BsnI GGCC 3 cut(s) 16, 45, 1210
Bsp1720I GCTNAGC 1 cut(s) 981
BspACI CCGC 7 cut(s) 75, 96, 423, 602, 855, 1017, 1122
BspANI GGCC 3 cut(s) 16, 45, 1210
BspCNI CTCAG 3 cut(s) 511, 670, 994
BspFNI CGCG 2 cut(s) 77, 602
BspLI GGNNCC 2 cut(s) 920, 1342
BspMAI CTGCAG 1 cut(s) 1293
BspMI ACCTGC 1 cut(s) 149
BspPI GGATC 3 cut(s) 115, 161, 233
BspTI CTTAAG 1 cut(s) 1367
BsrBI CCGCTC 1 cut(s) 423
BsrDI GCAATG 1 cut(s) 699
BsrFI RCCGGY 2 cut(s) 45, 890
BssAI RCCGGY 2 cut(s) 45, 890
BssECI CCNNGG 4 cut(s) 11, 292, 1047, 1069
BssNI GRCGYC 1 cut(s) 1176
BssSI CACGAG 1 cut(s) 416
BssT1I CCWWGG 2 cut(s) 292, 1047
Bst2BI CACGAG 1 cut(s) 416
Bst2UI CCWGG 1 cut(s) 1071
Bst4CI ACNGT 9 cut(s) 67, 169, 355, 410, 751, 1034, 1086, 1144, 1219
Bst6I CTCTTC 2 cut(s) 208, 248
BstACI GRCGYC 1 cut(s) 1176
BstAFI CTTAAG 1 cut(s) 1367
BstC8I GCNNGC 3 cut(s) 18, 47, 126
BstDEI CTNAG 4 cut(s) 215, 498, 657, 981
BstDSI CCRYGG 1 cut(s) 11
BstEII GGTNACC 2 cut(s) 1042, 1080
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 2 cut(s) 77, 602
BstHHI GCGC 2 cut(s) 85, 650
BstMAI GTCTC 3 cut(s) 250, 632, 690
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 1 cut(s) 938
BstNI CCWGG 1 cut(s) 1071
BstNSI RCATGY 2 cut(s) 448, 832
BstPI GGTNACC 2 cut(s) 1042, 1080
BstSCI CCNGG 2 cut(s) 874, 1069
BstSFI CTRYAG 4 cut(s) 135, 165, 226, 1289
BstUI CGCG 2 cut(s) 77, 602
BstV1I GCAGC 4 cut(s) 383, 529, 925, 1000
BstX2I RGATCY 4 cut(s) 107, 238, 486, 755
BstYI RGATCY 4 cut(s) 107, 238, 486, 755
BsuRI GGCC 3 cut(s) 16, 45, 1210
BtgI CCRYGG 1 cut(s) 11
BtgZI GCGATG 2 cut(s) 790, 1330
BtrI CACGTC 1 cut(s) 928
BtsCI GGATG 1 cut(s) 10
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 5 cut(s) 174, 657, 747, 1039, 1140
BveI ACCTGC 1 cut(s) 149
Cac8I GCNNGC 3 cut(s) 18, 47, 126
CfoI GCGC 2 cut(s) 85, 650
Cfr10I RCCGGY 2 cut(s) 45, 890
Cfr13I GGNCC 3 cut(s) 72, 161, 402
CseI GACGC 1 cut(s) 284
Csp6I GTAC 2 cut(s) 361, 1087
CspAI ACCGGT 1 cut(s) 890
CspCI CAANNNNNGTGG 2 cut(s) 298, 333
CviAII CATG 7 cut(s) 271, 445, 463, 469, 550, 829, 1153
CviQI GTAC 2 cut(s) 361, 1087
DdeI CTNAG 4 cut(s) 215, 498, 657, 981
EaeI YGGCCR 2 cut(s) 14, 43
Eam1104I CTCTTC 2 cut(s) 208, 248
EarI CTCTTC 2 cut(s) 208, 248
Eco130I CCWWGG 2 cut(s) 292, 1047
Eco32I GATATC 1 cut(s) 1149
Eco47I GGWCC 3 cut(s) 72, 161, 402
Eco91I GGTNACC 2 cut(s) 1042, 1080
EcoO109I RGGNCCY 1 cut(s) 161
EcoO65I GGTNACC 2 cut(s) 1042, 1080
EcoRII CCWGG 1 cut(s) 1069
EcoRV GATATC 1 cut(s) 1149
EcoT14I CCWWGG 2 cut(s) 292, 1047
ErhI CCWWGG 2 cut(s) 292, 1047
FaeI CATG 7 cut(s) 274, 448, 466, 472, 553, 832, 1156
FaqI GGGAC 1 cut(s) 76
FatI CATG 7 cut(s) 270, 444, 462, 468, 549, 828, 1152
FauI CCCGC 1 cut(s) 1115
FbaI TGATCA 2 cut(s) 465, 1317
Fnu4HI GCNGC 7 cut(s) 372, 543, 603, 855, 939, 1014, 1017
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 7 cut(s) 372, 543, 603, 855, 939, 1014, 1017
FspAI RTGCGCAY 1 cut(s) 84
FspBI CTAG 3 cut(s) 293, 1020, 1361
FspI TGCGCA 1 cut(s) 84
GlaI GCGC 2 cut(s) 84, 649
GluI GCNGC 7 cut(s) 372, 543, 603, 855, 939, 1014, 1017
HaeIII GGCC 3 cut(s) 16, 45, 1210
HapII CCGG 8 cut(s) 46, 234, 633, 875, 891, 1229, 1332, 1350
HgaI GACGC 1 cut(s) 284
HhaI GCGC 2 cut(s) 85, 650
Hin1I GRCGYC 1 cut(s) 1176
Hin1II CATG 7 cut(s) 274, 448, 466, 472, 553, 832, 1156
Hin6I GCGC 2 cut(s) 83, 648
HinP1I GCGC 2 cut(s) 83, 648
HincII GTYRAC 2 cut(s) 143, 1096
HindII GTYRAC 2 cut(s) 143, 1096
HinfI GANTC 7 cut(s) 325, 337, 588, 655, 770, 798, 1065
HpaII CCGG 8 cut(s) 46, 234, 633, 875, 891, 1229, 1332, 1350
HphI GGTGA 3 cut(s) 622, 1054, 1074
Hpy166II GTNNAC 6 cut(s) 143, 363, 1096, 1114, 1222, 1375
Hpy188I TCNGA 7 cut(s) 284, 501, 587, 660, 863, 1064, 1106
Hpy188III TCNNGA 5 cut(s) 310, 397, 743, 1028, 1387
Hpy8I GTNNAC 6 cut(s) 143, 363, 1096, 1114, 1222, 1375
Hpy99I CGWCG 2 cut(s) 1178, 1181
HpyAV CCTTC 3 cut(s) 565, 868, 1249
HpyCH4III ACNGT 9 cut(s) 67, 169, 355, 410, 751, 1034, 1086, 1144, 1219
HpyCH4IV ACGT 5 cut(s) 55, 927, 1098, 1176, 1322
HpyCH4V TGCA 2 cut(s) 569, 1291
HpyF10VI GCNNNNNNNGC 1 cut(s) 938
HpyF3I CTNAG 4 cut(s) 215, 498, 657, 981
HpySE526I ACGT 5 cut(s) 55, 927, 1098, 1176, 1322
Hsp92I GRCGYC 1 cut(s) 1176
Hsp92II CATG 7 cut(s) 274, 448, 466, 472, 553, 832, 1156
HspAI GCGC 2 cut(s) 83, 648
KroI GCCGGC 1 cut(s) 45
KroNI GCCGGC 1 cut(s) 47
Ksp22I TGATCA 2 cut(s) 465, 1317
LmnI GCTCC 3 cut(s) 924, 962, 1346
Lsp1109I GCAGC 4 cut(s) 383, 529, 925, 1000
LweI GCATC 3 cut(s) 383, 651, 813
MaeI CTAG 3 cut(s) 293, 1020, 1361
MaeII ACGT 5 cut(s) 55, 927, 1098, 1176, 1322
MaeIII GTNAC 8 cut(s) 190, 440, 478, 652, 811, 1034, 1042, 1080
MbiI CCGCTC 1 cut(s) 423
MboII GAAGA 4 cut(s) 195, 265, 796, 982
MflI RGATCY 4 cut(s) 107, 238, 486, 755
MluCI AATT 2 cut(s) 636, 738
MlyI GAGTC 1 cut(s) 649
MmeI TCCRAC 2 cut(s) 131, 429
MnlI CCTC 4 cut(s) 393, 419, 893, 1245
MroNI GCCGGC 1 cut(s) 45
MseI TTAA 3 cut(s) 51, 887, 1368
MslI CAYNNNNRTG 2 cut(s) 467, 1157
MspCI CTTAAG 1 cut(s) 1367
MspI CCGG 8 cut(s) 46, 234, 633, 875, 891, 1229, 1332, 1350
MspR9I CCNGG 2 cut(s) 876, 1071
MvaI CCWGG 1 cut(s) 1071
MvnI CGCG 2 cut(s) 77, 602
MwoI GCNNNNNNNGC 1 cut(s) 938
NaeI GCCGGC 1 cut(s) 47
NciI CCSGG 1 cut(s) 876
NgoMIV GCCGGC 1 cut(s) 45
NlaIII CATG 7 cut(s) 274, 448, 466, 472, 553, 832, 1156
NlaIV GGNNCC 2 cut(s) 920, 1342
NmuCI GTSAC 7 cut(s) 190, 440, 478, 652, 1034, 1042, 1080
NsbI TGCGCA 1 cut(s) 84
NspI RCATGY 2 cut(s) 448, 832
PciI ACATGT 1 cut(s) 444
PdiI GCCGGC 1 cut(s) 47
PfeI GAWTC 6 cut(s) 325, 337, 588, 770, 798, 1065
PflFI GACNNNGTC 1 cut(s) 278
PinAI ACCGGT 1 cut(s) 890
PkrI GCNGC 7 cut(s) 373, 544, 604, 856, 940, 1015, 1018
PleI GAGTC 1 cut(s) 649
PpsI GAGTC 1 cut(s) 649
PpuMI RGGWCCY 1 cut(s) 161
PscI ACATGT 1 cut(s) 444
Psp1406I AACGTT 2 cut(s) 55, 1098
Psp5II RGGWCCY 1 cut(s) 161
Psp6I CCWGG 1 cut(s) 1069
PspEI GGTNACC 2 cut(s) 1042, 1080
PspGI CCWGG 1 cut(s) 1069
PspN4I GGNNCC 2 cut(s) 920, 1342
PspPI GGNCC 3 cut(s) 72, 161, 402
PspPPI RGGWCCY 1 cut(s) 161
PstI CTGCAG 1 cut(s) 1293
PsuI RGATCY 4 cut(s) 107, 238, 486, 755
PsyI GACNNNGTC 1 cut(s) 278
RsaI GTAC 2 cut(s) 362, 1088
RsaNI GTAC 2 cut(s) 361, 1087
RseI CAYNNNNRTG 2 cut(s) 467, 1157
SaqAI TTAA 3 cut(s) 51, 887, 1368
SatI GCNGC 7 cut(s) 372, 543, 603, 855, 939, 1014, 1017
Sau96I GGNCC 3 cut(s) 72, 161, 402
SchI GAGTC 1 cut(s) 649
ScrFI CCNGG 2 cut(s) 876, 1071
SfaNI GCATC 3 cut(s) 383, 651, 813
SfcI CTRYAG 4 cut(s) 135, 165, 226, 1289
SinI GGWCC 3 cut(s) 72, 161, 402
SmiMI CAYNNNNRTG 2 cut(s) 467, 1157
SmlI CTYRAG 1 cut(s) 1367
SmoI CTYRAG 1 cut(s) 1367
Sse9I AATT 2 cut(s) 636, 738
SsiI CCGC 7 cut(s) 75, 96, 423, 602, 855, 1017, 1122
SspMI CTAG 3 cut(s) 293, 1020, 1361
StyD4I CCNGG 2 cut(s) 874, 1069
StyI CCWWGG 2 cut(s) 292, 1047
TaaI ACNGT 9 cut(s) 67, 169, 355, 410, 751, 1034, 1086, 1144, 1219
TaiI ACGT 5 cut(s) 58, 930, 1101, 1179, 1325
TaqI TCGA 1 cut(s) 449
TaqII GACCGA 1 cut(s) 497
TasI AATT 2 cut(s) 636, 738
TauI GCSGC 3 cut(s) 605, 857, 1019
TfiI GAWTC 6 cut(s) 325, 337, 588, 770, 798, 1065
Tru1I TTAA 3 cut(s) 51, 887, 1368
Tru9I TTAA 3 cut(s) 51, 887, 1368
TscAI CASTG 5 cut(s) 174, 657, 754, 1039, 1147
TseFI GTSAC 7 cut(s) 190, 440, 478, 652, 1034, 1042, 1080
TseI GCWGC 4 cut(s) 371, 542, 938, 1013
Tsp45I GTSAC 7 cut(s) 190, 440, 478, 652, 1034, 1042, 1080
TspDTI ATGAA 3 cut(s) 705, 797, 1289
TspGWI ACGGA 1 cut(s) 900
TspRI CASTG 5 cut(s) 174, 657, 754, 1039, 1147
Tth111I GACNNNGTC 1 cut(s) 278
Vha464I CTTAAG 1 cut(s) 1367
VpaK11BI GGWCC 3 cut(s) 72, 161, 402
XapI RAATTY 1 cut(s) 738
XceI RCATGY 2 cut(s) 448, 832
XmaJI CCTAGG 1 cut(s) 292
XspI CTAG 3 cut(s) 293, 1020, 1361
ZraI GACGTC 1 cut(s) 1177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.