MD05G1120400.v1.1

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014916
Physical Location & Seq
Reverse (-)
24217384 .. 24222371
4988 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1120400.v1.1.491

Sequence Viewer

Length: 1545 bp
ATGAAGCCAGTGTTGCCAAGAAGTAGAACTCTTCCCAGCAGAATCCACCATGGAGCTGTGGAAGAGAGGCATGACATCAGGCACTATCTGCAAGTGGAAGTCCAACCCAAAGTTAGTGAGTCCAAAGCCATAAACCCTCAGTCCAACTATTCCACATGCTTTGACGATGATGGTCGCTTGAAGAGAACAGGAACATTTTGGACTTCAGCATCACATATAATAACAGCGGTTATAGGATCTGGAGTCCTTTCCTTAGCATGGGCAATAGCACAGCTTGGTTGGGTTGCAGGACCAACTGTCCTCCTGCTCTTTGCCTTTGTCAATCTCTATACCTCCAATCTCCTAGCAATGTGTTACCGGACCGGCAACCCCATCACCGGACAGAGAAACTATACCTACATGGATGCTGTCAAGGCCAACTTAGGAGGAAGGAAGGTCATGATGTGTGGGTTGGTGCAGTACTTGAATTTATTTGGTGTAGCAATTGGGTACACAATTGCATCATCTGTCAGCATGATGGCAATAAAGAGATCAAATTGTTACCATAGAAGTGGAGGGAGAGATCCATGCCACATGTCAAGCAATGGGTATATGATAACATTTGGTATCATAGAGGTGATATTTTCTCAAATCCCAGATTTTAATCAAGTGTGGTGGCTCTCAATAGTAGCAGCAATTATGTCATTTACTTATTCTTCTGTTGGCCTTGGACTTGGCATTGACAAAGTTGCAGGAAATGGGAGCTTTAAAGGGAGCCTATTGGGTATTAGCATAGGGACCGTAACCAAGAGTGGAACAGTGACTTCTACACAAAAGATATGGAGAAGTATGCAAGCTCTTGGAGCTATTGCCTTTGCTTACTCTTATTCTTTGGTCCTCATTGAAATTCAGGATACAATAAGATCTCCACCCGTAGAACATAAGACAATGAAGAAGGCAACTGTATTCAGCATAGCTATCACCACAGTGTTCTATCTACTTTGTGGATGCTTTGGGTATGCAGCCTTTGGTGACTTGGCCCCAGGAAACCTCTTGACTGGCTTTGGATTTTACAACCCCTACTGGCTACTAGACATTGCCAATGTTGCAATTGTTGTCCACCTTGTTGGTGCATTTCAGGTCTTTTGTCAACCATTATTTGCATTTGTAGAAAAATGGAGCGCAAAGAGGTGGCCAAACAGCGACTTTGTGACAGCAGAGTATGAGATACCGATCCCCTTCTACGGCGTCTACCAACTCAACCTCTTCCGCTTGGTATGGAGGACAATATTCGTCATAATAACAACCCTCATATCCATGCTCCTGCCCTTCTTCAACGACGTCGTTGGAATACTTGGAGCCTTTGGATTTTGGCCACTCACAGTTTACTTCCCAGTCGAGATGTACATTGCTCAGGAGAAGATTCGGAAGTGGAGCAGCAAATGGGTTGGACTTCAGATGCTCAGTGTAAGTTGTCTTTTCGTCTCCATAGCTGCTGCTGTTGGCTCTGTGGCTGGTGTGGTTCTTGATCTCCAGACTTATAAGCCCTTTAAAACTAGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

515

Amino Acids

56.94

Weight (kDa)

9.27

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 62 - 501 2.1e-116 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09220 AT5G63850
fragaria_vesca FvH4_2g01330
malus_domestica MD05G1120400.v1.1
prunus_persica Prupe.4G277700_v2.0.a1 Prupe.8G164200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0252251
rosa_laevigata RLG00000015401
rosa_multiflora Rmu_sc0006252.1_g000018 Rmu_sc0009799.1_g000021
rosa_roxburghii Rroxscaffold_7G00216210
rosa_rugosa Rorug05G0506400
rosa_samantha Rh6AG020200 Rh6BG015800 Rh6CG013000
rosa_wichuraiana Rw6G001660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1521
AatII GACGTC 1 cut(s) 1323
AccI GTMKAC 1 cut(s) 1230
AciI CCGC 2 cut(s) 227, 1249
AclWI GGATC 3 cut(s) 244, 557, 1207
AcoI YGGCCR 2 cut(s) 1172, 1352
AcsI RAATTY 2 cut(s) 466, 885
AcuI CTGAAG 2 cut(s) 189, 1418
AcyI GRCGYC 2 cut(s) 1227, 1320
AfaI GTAC 3 cut(s) 461, 491, 1385
AfiI CCNNNNNNNGG 3 cut(s) 258, 377, 1223
AflIII ACRYGT 1 cut(s) 573
AgsI TTSAA 4 cut(s) 181, 466, 884, 1315
AhdI GACNNNNNGTC 1 cut(s) 296
AhlI ACTAGT 1 cut(s) 1535
AjnI CCWGG 1 cut(s) 1021
AleI CACNNNNGTG 1 cut(s) 965
AluBI AGCT 7 cut(s) 56, 274, 744, 836, 845, 956, 1472
AluI AGCT 7 cut(s) 56, 274, 744, 836, 845, 956, 1472
Alw26I GTCTC 1 cut(s) 1468
AlwI GGATC 3 cut(s) 244, 557, 1207
AoxI GGCC 5 cut(s) 414, 703, 1017, 1172, 1352
ApeKI GCWGC 5 cut(s) 671, 1001, 1416, 1472, 1475
ApoI RAATTY 2 cut(s) 466, 885
AspLEI GCGC 1 cut(s) 1163
AspS9I GGNCC 5 cut(s) 290, 360, 777, 874, 1018
AsuHPI GGTGA 4 cut(s) 367, 628, 952, 1022
AvaII GGWCC 4 cut(s) 290, 360, 777, 874
BalI TGGCCA 2 cut(s) 1174, 1354
BbvI GCAGC 5 cut(s) 683, 1013, 1428, 1459, 1462
BccI CCATC 3 cut(s) 164, 380, 511
BceAI ACGGC 1 cut(s) 1240
BciT130I CCWGG 1 cut(s) 1023
BciVI GTATCC 1 cut(s) 886
BcoDI GTCTC 1 cut(s) 1468
BcuI ACTAGT 1 cut(s) 1535
BfaI CTAG 3 cut(s) 344, 1070, 1536
BfuI GTATCC 1 cut(s) 886
BglII AGATCT 1 cut(s) 902
BisI GCNGC 5 cut(s) 672, 1002, 1417, 1473, 1476
BlsI GCNGC 5 cut(s) 673, 1003, 1418, 1474, 1477
BmcAI AGTACT 1 cut(s) 461
Bme1390I CCNGG 1 cut(s) 1023
Bme18I GGWCC 4 cut(s) 290, 360, 777, 874
BmeRI GACNNNNNGTC 1 cut(s) 296
BmgT120I GGNCC 5 cut(s) 290, 360, 777, 874, 1018
BmiI GGNNCC 4 cut(s) 755, 778, 1020, 1339
BmrFI CCNGG 1 cut(s) 1023
BmrI ACTGGG 1 cut(s) 1367
BmsI GCATC 5 cut(s) 218, 394, 509, 977, 1428
BmuI ACTGGG 1 cut(s) 1367
BpmI CTGGAG 2 cut(s) 261, 1496
Bpu10I CCTNAGC 2 cut(s) 253, 1392
BsaBI GATNNNNATC 2 cut(s) 642, 1211
BsaHI GRCGYC 2 cut(s) 1227, 1320
BsaJI CCNNGG 3 cut(s) 49, 706, 1021
BsaWI WCCGGW 2 cut(s) 357, 377
Bsc4I CCNNNNNNNGG 3 cut(s) 258, 377, 1223
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 4 cut(s) 8, 1042, 1067, 1373
Bse3DI GCAATG 4 cut(s) 354, 589, 1074, 1386
Bse8I GATNNNNATC 2 cut(s) 642, 1211
BseBI CCWGG 1 cut(s) 1023
BseDI CCNNGG 3 cut(s) 49, 706, 1021
BseGI GGATG 2 cut(s) 409, 992
BseJI GATNNNNATC 2 cut(s) 642, 1211
BseLI CCNNNNNNNGG 3 cut(s) 258, 377, 1223
BseMI GCAATG 4 cut(s) 354, 589, 1074, 1386
BseMII CTCAG 3 cut(s) 152, 1406, 1456
BseNI ACTGG 4 cut(s) 8, 1042, 1067, 1373
BseXI GCAGC 5 cut(s) 683, 1013, 1428, 1459, 1462
BseYI CCCAGC 1 cut(s) 35
BsgI GTGCAG 1 cut(s) 476
BshFI GGCC 5 cut(s) 416, 705, 1019, 1174, 1354
BsiSI CCGG 3 cut(s) 358, 363, 378
BslFI GGGAC 1 cut(s) 790
BslI CCNNNNNNNGG 3 cut(s) 258, 377, 1223
BsmAI GTCTC 1 cut(s) 1468
BsmBI CGTCTC 1 cut(s) 1468
BsmFI GGGAC 1 cut(s) 790
BsnI GGCC 5 cut(s) 416, 705, 1019, 1174, 1354
Bsp1407I TGTACA 1 cut(s) 1383
Bsp143I GATC 6 cut(s) 236, 530, 562, 902, 1212, 1507
Bsp19I CCATGG 1 cut(s) 49
BspACI CCGC 2 cut(s) 227, 1249
BspANI GGCC 5 cut(s) 416, 705, 1019, 1174, 1354
BspCNI CTCAG 3 cut(s) 151, 1405, 1455
BspHI TCATGA 1 cut(s) 438
BspLI GGNNCC 4 cut(s) 755, 778, 1020, 1339
BspPI GGATC 3 cut(s) 244, 557, 1207
BsrDI GCAATG 4 cut(s) 354, 589, 1074, 1386
BsrFI RCCGGY 1 cut(s) 362
BsrGI TGTACA 1 cut(s) 1383
BsrI ACTGG 4 cut(s) 8, 1042, 1067, 1373
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 3 cut(s) 49, 706, 1021
BssMI GATC 6 cut(s) 236, 530, 562, 902, 1212, 1507
BssNI GRCGYC 2 cut(s) 1227, 1320
BssT1I CCWWGG 2 cut(s) 49, 706
Bst2UI CCWGG 1 cut(s) 1023
Bst4CI ACNGT 6 cut(s) 298, 781, 799, 943, 967, 1363
Bst6I CTCTTC 4 cut(s) 36, 57, 176, 1250
BstACI GRCGYC 2 cut(s) 1227, 1320
BstAPI GCANNNNNTGC 1 cut(s) 88
BstAUI TGTACA 1 cut(s) 1383
BstC8I GCNNGC 1 cut(s) 834
BstDEI CTNAG 5 cut(s) 138, 253, 421, 1392, 1442
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 2 cut(s) 409, 992
BstHHI GCGC 1 cut(s) 1163
BstKTI GATC 6 cut(s) 239, 533, 565, 905, 1215, 1510
BstMAI GTCTC 1 cut(s) 1468
BstMBI GATC 6 cut(s) 236, 530, 562, 902, 1212, 1507
BstMWI GCNNNNNNNGC 5 cut(s) 13, 88, 413, 842, 1085
BstNI CCWGG 1 cut(s) 1023
BstNSI RCATGY 2 cut(s) 159, 577
BstSCI CCNGG 1 cut(s) 1021
BstV1I GCAGC 5 cut(s) 683, 1013, 1428, 1459, 1462
BstX2I RGATCY 3 cut(s) 236, 562, 902
BstXI CCANNNNNNTGG 2 cut(s) 551, 1106
BstYI RGATCY 3 cut(s) 236, 562, 902
BsuI GTATCC 1 cut(s) 886
BsuRI GGCC 5 cut(s) 416, 705, 1019, 1174, 1354
BtgI CCRYGG 1 cut(s) 49
BtsCI GGATG 2 cut(s) 409, 992
BtsIMutI CAGTG 4 cut(s) 15, 804, 972, 1450
Cac8I GCNNGC 1 cut(s) 834
CciI TCATGA 1 cut(s) 438
CfoI GCGC 1 cut(s) 1163
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 5 cut(s) 290, 360, 777, 874, 1018
CpoI CGGWCCG 1 cut(s) 360
CseI GACGC 1 cut(s) 1216
Csp6I GTAC 3 cut(s) 460, 490, 1384
CspCI CAANNNNNGTGG 4 cut(s) 142, 177, 635, 670
CspI CGGWCCG 1 cut(s) 360
CviQI GTAC 3 cut(s) 460, 490, 1384
DdeI CTNAG 5 cut(s) 138, 253, 421, 1392, 1442
DpnI GATC 6 cut(s) 238, 532, 564, 904, 1214, 1509
DpnII GATC 6 cut(s) 236, 530, 562, 902, 1212, 1507
DraI TTTAAA 2 cut(s) 748, 1531
DriI GACNNNNNGTC 1 cut(s) 296
EaeI YGGCCR 2 cut(s) 1172, 1352
Eam1104I CTCTTC 4 cut(s) 36, 57, 176, 1250
Eam1105I GACNNNNNGTC 1 cut(s) 296
EarI CTCTTC 4 cut(s) 36, 57, 176, 1250
Eco130I CCWWGG 2 cut(s) 49, 706
Eco47I GGWCC 4 cut(s) 290, 360, 777, 874
Eco57I CTGAAG 2 cut(s) 189, 1418
EcoRII CCWGG 1 cut(s) 1021
EcoT14I CCWWGG 2 cut(s) 49, 706
ErhI CCWWGG 2 cut(s) 49, 706
Esp3I CGTCTC 1 cut(s) 1468
FalI AAGNNNNNCTT 2 cut(s) 404, 436
FaqI GGGAC 1 cut(s) 790
FblI GTMKAC 1 cut(s) 1230
Fnu4HI GCNGC 5 cut(s) 672, 1002, 1417, 1473, 1476
FokI GGATG 2 cut(s) 416, 999
Fsp4HI GCNGC 5 cut(s) 672, 1002, 1417, 1473, 1476
FspBI CTAG 3 cut(s) 344, 1070, 1536
GlaI GCGC 1 cut(s) 1162
GluI GCNGC 5 cut(s) 672, 1002, 1417, 1473, 1476
GsaI CCCAGC 1 cut(s) 39
GsuI CTGGAG 2 cut(s) 261, 1496
HaeIII GGCC 5 cut(s) 416, 705, 1019, 1174, 1354
HapII CCGG 3 cut(s) 358, 363, 378
HgaI GACGC 1 cut(s) 1216
HhaI GCGC 1 cut(s) 1163
Hin1I GRCGYC 2 cut(s) 1227, 1320
Hin6I GCGC 1 cut(s) 1161
HinP1I GCGC 1 cut(s) 1161
HincII GTYRAC 1 cut(s) 1130
HindII GTYRAC 1 cut(s) 1130
HinfI GANTC 4 cut(s) 42, 119, 243, 1402
HpaII CCGG 3 cut(s) 358, 363, 378
HphI GGTGA 4 cut(s) 367, 628, 952, 1022
Hpy166II GTNNAC 5 cut(s) 492, 1099, 1130, 1231, 1366
Hpy188I TCNGA 2 cut(s) 1407, 1437
Hpy188III TCNNGA 8 cut(s) 240, 439, 890, 1033, 1378, 1394, 1505, 1513
Hpy8I GTNNAC 5 cut(s) 492, 1099, 1130, 1231, 1366
Hpy99I CGWCG 2 cut(s) 1322, 1325
HpyAV CCTTC 5 cut(s) 423, 427, 928, 1228, 1318
HpyCH4III ACNGT 6 cut(s) 298, 781, 799, 943, 967, 1363
HpyCH4IV ACGT 1 cut(s) 1320
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 88, 413, 842, 1085
HpyF3I CTNAG 5 cut(s) 138, 253, 421, 1392, 1442
HpySE526I ACGT 1 cut(s) 1320
Hsp92I GRCGYC 2 cut(s) 1227, 1320
HspAI GCGC 1 cut(s) 1161
Kzo9I GATC 6 cut(s) 236, 530, 562, 902, 1212, 1507
LmnI GCTCC 8 cut(s) 53, 741, 753, 842, 1158, 1305, 1337, 1413
Lsp1109I GCAGC 5 cut(s) 683, 1013, 1428, 1459, 1462
LweI GCATC 5 cut(s) 218, 394, 509, 977, 1428
MaeI CTAG 3 cut(s) 344, 1070, 1536
MaeII ACGT 1 cut(s) 1320
MaeIII GTNAC 7 cut(s) 353, 539, 781, 799, 1010, 1189, 1538
MalI GATC 6 cut(s) 238, 532, 564, 904, 1214, 1509
MboI GATC 6 cut(s) 236, 530, 562, 902, 1212, 1507
MboII GAAGA 8 cut(s) 23, 74, 193, 687, 943, 1237, 1303, 1411
MfeI CAATTG 3 cut(s) 483, 495, 1089
MflI RGATCY 3 cut(s) 236, 562, 902
MlsI TGGCCA 2 cut(s) 1174, 1354
MluCI AATT 7 cut(s) 466, 483, 495, 535, 675, 885, 1089
MluNI TGGCCA 2 cut(s) 1174, 1354
MlyI GAGTC 2 cut(s) 128, 252
MmeI TCCRAC 4 cut(s) 127, 168, 1306, 1408
Mox20I TGGCCA 2 cut(s) 1174, 1354
MscI TGGCCA 2 cut(s) 1174, 1354
MseI TTAA 3 cut(s) 642, 747, 1530
MslI CAYNNNNRTG 4 cut(s) 549, 614, 965, 1295
Msp20I TGGCCA 2 cut(s) 1174, 1354
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 3 cut(s) 358, 363, 378
MspR9I CCNGG 1 cut(s) 1023
MunI CAATTG 3 cut(s) 483, 495, 1089
MvaI CCWGG 1 cut(s) 1023
MwoI GCNNNNNNNGC 5 cut(s) 13, 88, 413, 842, 1085
NcoI CCATGG 1 cut(s) 49
NdeII GATC 6 cut(s) 236, 530, 562, 902, 1212, 1507
NlaIV GGNNCC 4 cut(s) 755, 778, 1020, 1339
NmuCI GTSAC 3 cut(s) 799, 1010, 1189
NspI RCATGY 2 cut(s) 159, 577
OliI CACNNNNGTG 1 cut(s) 965
PagI TCATGA 1 cut(s) 438
PciI ACATGT 1 cut(s) 573
PfeI GAWTC 2 cut(s) 42, 1402
PkrI GCNGC 5 cut(s) 673, 1003, 1418, 1474, 1477
PleI GAGTC 2 cut(s) 127, 251
PpsI GAGTC 2 cut(s) 127, 251
PscI ACATGT 1 cut(s) 573
PsiI TTATAA 1 cut(s) 1521
Psp6I CCWGG 1 cut(s) 1021
PspFI CCCAGC 1 cut(s) 35
PspGI CCWGG 1 cut(s) 1021
PspN4I GGNNCC 4 cut(s) 755, 778, 1020, 1339
PspPI GGNCC 5 cut(s) 290, 360, 777, 874, 1018
PsuI RGATCY 3 cut(s) 236, 562, 902
RsaI GTAC 3 cut(s) 461, 491, 1385
RsaNI GTAC 3 cut(s) 460, 490, 1384
RseI CAYNNNNRTG 4 cut(s) 549, 614, 965, 1295
Rsr2I CGGWCCG 1 cut(s) 360
RsrII CGGWCCG 1 cut(s) 360
SaqAI TTAA 3 cut(s) 642, 747, 1530
SatI GCNGC 5 cut(s) 672, 1002, 1417, 1473, 1476
Sau3AI GATC 6 cut(s) 236, 530, 562, 902, 1212, 1507
Sau96I GGNCC 5 cut(s) 290, 360, 777, 874, 1018
ScaI AGTACT 1 cut(s) 461
SchI GAGTC 2 cut(s) 128, 252
ScrFI CCNGG 1 cut(s) 1023
SfaNI GCATC 5 cut(s) 218, 394, 509, 977, 1428
SinI GGWCC 4 cut(s) 290, 360, 777, 874
SmiMI CAYNNNNRTG 4 cut(s) 549, 614, 965, 1295
SpeI ACTAGT 1 cut(s) 1535
Sse9I AATT 7 cut(s) 466, 483, 495, 535, 675, 885, 1089
SsiI CCGC 2 cut(s) 227, 1249
SspI AATATT 1 cut(s) 1269
SspMI CTAG 3 cut(s) 344, 1070, 1536
StyD4I CCNGG 1 cut(s) 1021
StyI CCWWGG 2 cut(s) 49, 706
TaaI ACNGT 6 cut(s) 298, 781, 799, 943, 967, 1363
TaiI ACGT 1 cut(s) 1323
TaqI TCGA 1 cut(s) 1377
TasI AATT 7 cut(s) 466, 483, 495, 535, 675, 885, 1089
TatI WGTACW 2 cut(s) 459, 1383
TfiI GAWTC 2 cut(s) 42, 1402
Tru1I TTAA 3 cut(s) 642, 747, 1530
Tru9I TTAA 3 cut(s) 642, 747, 1530
TscAI CASTG 4 cut(s) 15, 804, 972, 1450
TseFI GTSAC 3 cut(s) 799, 1010, 1189
TseI GCWGC 5 cut(s) 671, 1001, 1416, 1472, 1475
Tsp45I GTSAC 3 cut(s) 799, 1010, 1189
TspDTI ATGAA 2 cut(s) 17, 944
TspRI CASTG 4 cut(s) 15, 804, 972, 1450
VpaK11BI GGWCC 4 cut(s) 290, 360, 777, 874
XapI RAATTY 2 cut(s) 466, 885
XceI RCATGY 2 cut(s) 159, 577
XmiI GTMKAC 1 cut(s) 1230
XspI CTAG 3 cut(s) 344, 1070, 1536
ZraI GACGTC 1 cut(s) 1321
ZrmI AGTACT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.