Prupe.8G164200_v2.0.a1

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0014916
Physical Location & Seq
Reverse (-)
17116084 .. 17120089
4006 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G164200.2

Sequence Viewer

Length: 1536 bp
ATGTTGCAAAGAAGTAGAACTCTTCCAAGCAGAATCCACCATGGAGCTGTGGAAGAGAGGCATGACATCAGGCACTACTTGCAAGTGGAAGTCCAACCCAAAGTCACTGAGTCTGAAGCCATAAACCCTCAGTCCAGCTATTCCAAATGCTTTGATGATGATGGTCGCCTGAAGAGAACAGGAAATTTTTGGACTTCAGCGTCACATATTATCACAGCAGTTATAGGATCTGGTGTCCTTTCCTTAGCATGGGCAATAGCTCAGCTTGGTTGGGTTGCAGGACCATCTGTCCTGCTGCTTTTTGCCCTTGTTAATCTCTACACTTCCAATCTCCTAGCAATGTGCTACCGGTCCGGTGACCCTGTCACAGGACAGAGAAATTATACCTACATGGATGCTATCAAGGCCAACTTAGGAGGAAGAAAGGTCATGTTATGTGGGTTGGTCCAGTACTTCAATCTATTTGGTGTAGCAATTGGTTACACCATTGCCTCATCTGTCAGCATGATGGCAATAAAGAGATCAAACTGTTACCATAAAAGTGGAGGGAAAGATCCATGCCACATGTCAAGCAATGGGTACATGATAACATTTGGAATCATAGAGGTGATATTTTCTCAGATCCCAGATTTTAATCAAGTATGGTGGCTCTCAATAGTAGCTGCCATTATGTCCTTCACATACTCCTCTGTTGGCCTCGGACTTGGCATTGGCAAAGTTGTAGGAAATGGGGGATTTAAAGGAAGCCTACTGGGTATCAGCATAGGGACAGTAACCCATGCAGGAACAGTTACTCCTACACAAAAGATGTGGAGGAGCATGCAAGCTCTTGGAGCTATTGCCTTTGCTTACTCTTATTCTTTGGTTCTCATTGAAATTCAGGATACAATAAGATCTCCTCCTGCAGAACATAAGACCATGAAGAAGGCAACTGTATTCAGCATAGCTCTGACCACAGTGTTCTATCTATTCTGTGGTTGCTTTGGGTATGCAGCCTTTGGTGACCTGGCCCCAGGCAACCTCTTGACTGGCTTTGGATTCTACAAACCTTACTGGCTACTAGATATTGCCAATGTTGCTATTGTTGTCCACCTTGTTGGTGCATTTCAGGTCTACTGCCAGCCTTTATTTGCATTTGTGGAGAAATGGAGCGCACAGAGGTGGCCAAAGAGGGACTTTGTAACTGCAGAATATGACATACCAATCCCTTTCTATGGCGTCTACCAACTCAACCTGTTTCGCTTAGTATGGAGGACCATATTTGTCATGATAACAACCCTCATATCCATGCTGCTGCCTTTCTTCAATGATGTGGTTGGAATTCTTGGAGCCTTTGGGTTCTGGCCATTGACAGTTTACTTCCCAGTTGAGATGTACATTGCTCAACAGAAGATTGGGAAATGGAGCAGCAGATGGGTTGGGCTTAAGATGCTTAGCATCAGTTGTCTCCTTGTCACCATAGCTGCTGCAGTCGGTTCTGTGGCTGGTGTGGTTCTGGATCTCAAGACTTATAAACCCTTTAAAACTAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

512

Amino Acids

56.58

Weight (kDa)

9.3

Isoelectric Point (pI)

37.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09220 AT5G63850
fragaria_vesca FvH4_2g01330
malus_domestica MD05G1120400.v1.1
prunus_persica Prupe.4G277700_v2.0.a1 Prupe.8G164200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0252251
rosa_laevigata RLG00000015401
rosa_multiflora Rmu_sc0006252.1_g000018 Rmu_sc0009799.1_g000021
rosa_roxburghii Rroxscaffold_7G00216210
rosa_rugosa Rorug05G0506400
rosa_samantha Rh6AG020200 Rh6BG015800 Rh6CG013000
rosa_wichuraiana Rw6G001660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1512
AasI GACNNNNNNGTC 1 cut(s) 199
AccI GTMKAC 2 cut(s) 1113, 1221
AclWI GGATC 4 cut(s) 235, 548, 616, 1506
AcoI YGGCCR 2 cut(s) 1163, 1343
AcsI RAATTY 3 cut(s) 184, 876, 1320
AcuI CTGAAG 3 cut(s) 135, 180, 191
AcyI GRCGYC 1 cut(s) 1218
AfaI GTAC 3 cut(s) 452, 581, 1376
AfiI CCNNNNNNNGG 3 cut(s) 249, 368, 1214
AflII CTTAAG 1 cut(s) 1424
AflIII ACRYGT 1 cut(s) 564
AgeI ACCGGT 1 cut(s) 348
AgsI TTSAA 3 cut(s) 457, 875, 1306
AhdI GACNNNNNGTC 1 cut(s) 287
AjnI CCWGG 2 cut(s) 1005, 1012
AleI CACNNNNGTG 1 cut(s) 1159
AloI GAACNNNNNNTCC 2 cut(s) 778, 810
Alw26I GTCTC 1 cut(s) 1451
AlwI GGATC 4 cut(s) 235, 548, 616, 1506
AoxI GGCC 5 cut(s) 405, 694, 1008, 1163, 1343
ApeKI GCWGC 8 cut(s) 295, 662, 992, 1291, 1294, 1407, 1463, 1466
ApoI RAATTY 3 cut(s) 184, 876, 1320
AsiGI ACCGGT 1 cut(s) 348
AspLEI GCGC 1 cut(s) 1154
AspS9I GGNCC 5 cut(s) 281, 351, 445, 1009, 1254
AsuHPI GGTGA 4 cut(s) 368, 619, 1013, 1447
AvaII GGWCC 4 cut(s) 281, 351, 445, 1254
BalI TGGCCA 2 cut(s) 1165, 1345
BbvI GCAGC 8 cut(s) 282, 649, 1004, 1278, 1281, 1419, 1450, 1453
BccI CCATC 4 cut(s) 155, 292, 502, 1407
BciT130I CCWGG 2 cut(s) 1007, 1014
BciVI GTATCC 1 cut(s) 877
BcoDI GTCTC 1 cut(s) 1451
BfaI CTAG 3 cut(s) 335, 1061, 1527
BfmI CTRYAG 3 cut(s) 903, 1185, 1467
BfrI CTTAAG 1 cut(s) 1424
BfuI GTATCC 1 cut(s) 877
BglII AGATCT 1 cut(s) 893
BisI GCNGC 8 cut(s) 296, 663, 993, 1292, 1295, 1408, 1464, 1467
BlpI GCTNAGC 2 cut(s) 261, 1433
BlsI GCNGC 8 cut(s) 297, 664, 994, 1293, 1296, 1409, 1465, 1468
BmcAI AGTACT 1 cut(s) 452
Bme1390I CCNGG 2 cut(s) 1007, 1014
Bme18I GGWCC 4 cut(s) 281, 351, 445, 1254
BmeRI GACNNNNNGTC 1 cut(s) 287
BmgT120I GGNCC 5 cut(s) 281, 351, 445, 1009, 1254
BmiI GGNNCC 2 cut(s) 1011, 1330
BmrFI CCNGG 2 cut(s) 1007, 1014
BmrI ACTGGG 2 cut(s) 761, 1358
BmsI GCATC 3 cut(s) 385, 1419, 1446
BmuI ACTGGG 2 cut(s) 761, 1358
Bpu10I CCTNAGC 1 cut(s) 244
Bpu1102I GCTNAGC 2 cut(s) 261, 1433
BpuEI CTTGAG 1 cut(s) 1487
BsaBI GATNNNNATC 1 cut(s) 633
BsaHI GRCGYC 1 cut(s) 1218
BsaJI CCNNGG 3 cut(s) 40, 697, 1012
BsaWI WCCGGW 2 cut(s) 348, 353
BsaXI ACNNNNNCTCC 2 cut(s) 778, 808
Bsc4I CCNNNNNNNGG 3 cut(s) 249, 368, 1214
Bse118I RCCGGY 1 cut(s) 348
Bse1I ACTGG 5 cut(s) 448, 756, 1033, 1058, 1364
Bse3DI GCAATG 4 cut(s) 345, 486, 580, 1377
Bse8I GATNNNNATC 1 cut(s) 633
BseBI CCWGG 2 cut(s) 1007, 1014
BseDI CCNNGG 3 cut(s) 40, 697, 1012
BseGI GGATG 1 cut(s) 400
BseJI GATNNNNATC 1 cut(s) 633
BseLI CCNNNNNNNGG 3 cut(s) 249, 368, 1214
BseMI GCAATG 4 cut(s) 345, 486, 580, 1377
BseMII CTCAG 4 cut(s) 99, 143, 275, 632
BseNI ACTGG 5 cut(s) 448, 756, 1033, 1058, 1364
BseRI GAGGAG 3 cut(s) 676, 829, 888
BseXI GCAGC 8 cut(s) 282, 649, 1004, 1278, 1281, 1419, 1450, 1453
BshFI GGCC 5 cut(s) 407, 696, 1010, 1165, 1345
BshTI ACCGGT 1 cut(s) 348
BsiSI CCGG 2 cut(s) 349, 354
BslFI GGGAC 2 cut(s) 781, 1187
BslI CCNNNNNNNGG 3 cut(s) 249, 368, 1214
BsmAI GTCTC 1 cut(s) 1451
BsmFI GGGAC 2 cut(s) 781, 1187
BsnI GGCC 5 cut(s) 407, 696, 1010, 1165, 1345
Bsp1407I TGTACA 1 cut(s) 1374
Bsp143I GATC 6 cut(s) 227, 521, 553, 621, 893, 1498
Bsp1720I GCTNAGC 2 cut(s) 261, 1433
Bsp19I CCATGG 1 cut(s) 40
BspANI GGCC 5 cut(s) 407, 696, 1010, 1165, 1345
BspCNI CTCAG 4 cut(s) 100, 142, 274, 631
BspHI TCATGA 1 cut(s) 1266
BspLI GGNNCC 2 cut(s) 1011, 1330
BspMAI CTGCAG 3 cut(s) 907, 1189, 1471
BspPI GGATC 4 cut(s) 235, 548, 616, 1506
BspTI CTTAAG 1 cut(s) 1424
BsrDI GCAATG 4 cut(s) 345, 486, 580, 1377
BsrFI RCCGGY 1 cut(s) 348
BsrGI TGTACA 1 cut(s) 1374
BsrI ACTGG 5 cut(s) 448, 756, 1033, 1058, 1364
BssAI RCCGGY 1 cut(s) 348
BssECI CCNNGG 3 cut(s) 40, 697, 1012
BssMI GATC 6 cut(s) 227, 521, 553, 621, 893, 1498
BssNI GRCGYC 1 cut(s) 1218
BssT1I CCWWGG 1 cut(s) 40
Bst2UI CCWGG 2 cut(s) 1007, 1014
Bst4CI ACNGT 6 cut(s) 530, 772, 790, 934, 958, 1354
Bst6I CTCTTC 3 cut(s) 27, 48, 167
BstACI GRCGYC 1 cut(s) 1218
BstAFI CTTAAG 1 cut(s) 1424
BstAPI GCANNNNNTGC 1 cut(s) 79
BstAUI TGTACA 1 cut(s) 1374
BstC8I GCNNGC 3 cut(s) 821, 825, 1121
BstDEI CTNAG 8 cut(s) 108, 129, 244, 261, 412, 618, 1243, 1433
BstDSI CCRYGG 1 cut(s) 40
BstEII GGTNACC 2 cut(s) 356, 1001
BstENI CCTNNNNNAGG 1 cut(s) 366
BstF5I GGATG 1 cut(s) 400
BstHHI GCGC 1 cut(s) 1154
BstKTI GATC 6 cut(s) 230, 524, 556, 624, 896, 1501
BstMAI GTCTC 1 cut(s) 1451
BstMBI GATC 6 cut(s) 227, 521, 553, 621, 893, 1498
BstMWI GCNNNNNNNGC 5 cut(s) 79, 404, 833, 1076, 1429
BstNI CCWGG 2 cut(s) 1007, 1014
BstNSI RCATGY 2 cut(s) 568, 823
BstPI GGTNACC 2 cut(s) 356, 1001
BstSCI CCNGG 2 cut(s) 1005, 1012
BstSFI CTRYAG 3 cut(s) 903, 1185, 1467
BstV1I GCAGC 8 cut(s) 282, 649, 1004, 1278, 1281, 1419, 1450, 1453
BstX2I RGATCY 5 cut(s) 227, 553, 621, 893, 1498
BstXI CCANNNNNNTGG 2 cut(s) 542, 1097
BstYI RGATCY 5 cut(s) 227, 553, 621, 893, 1498
BsuI GTATCC 1 cut(s) 877
BsuRI GGCC 5 cut(s) 407, 696, 1010, 1165, 1345
BtgI CCRYGG 1 cut(s) 40
BtsCI GGATG 1 cut(s) 400
BtsIMutI CAGTG 2 cut(s) 105, 963
Cac8I GCNNGC 3 cut(s) 821, 825, 1121
CciI TCATGA 1 cut(s) 1266
CfoI GCGC 1 cut(s) 1154
Cfr10I RCCGGY 1 cut(s) 348
Cfr13I GGNCC 5 cut(s) 281, 351, 445, 1009, 1254
CpoI CGGWCCG 1 cut(s) 351
CseI GACGC 2 cut(s) 189, 1207
Csp6I GTAC 3 cut(s) 451, 580, 1375
CspAI ACCGGT 1 cut(s) 348
CspCI CAANNNNNGTGG 4 cut(s) 626, 661, 791, 826
CspI CGGWCCG 1 cut(s) 351
CviQI GTAC 3 cut(s) 451, 580, 1375
DdeI CTNAG 8 cut(s) 108, 129, 244, 261, 412, 618, 1243, 1433
DpnI GATC 6 cut(s) 229, 523, 555, 623, 895, 1500
DpnII GATC 6 cut(s) 227, 521, 553, 621, 893, 1498
DraI TTTAAA 2 cut(s) 739, 1522
DrdI GACNNNNNNGTC 1 cut(s) 199
DriI GACNNNNNGTC 1 cut(s) 287
DseDI GACNNNNNNGTC 1 cut(s) 199
EaeI YGGCCR 2 cut(s) 1163, 1343
Eam1104I CTCTTC 3 cut(s) 27, 48, 167
Eam1105I GACNNNNNGTC 1 cut(s) 287
EarI CTCTTC 3 cut(s) 27, 48, 167
Eco130I CCWWGG 1 cut(s) 40
Eco47I GGWCC 4 cut(s) 281, 351, 445, 1254
Eco57I CTGAAG 3 cut(s) 135, 180, 191
Eco91I GGTNACC 2 cut(s) 356, 1001
EcoNI CCTNNNNNAGG 1 cut(s) 366
EcoO65I GGTNACC 2 cut(s) 356, 1001
EcoRI GAATTC 1 cut(s) 1320
EcoRII CCWGG 2 cut(s) 1005, 1012
EcoT14I CCWWGG 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 40
FalI AAGNNNNNCTT 4 cut(s) 395, 427, 1160, 1192
FaqI GGGAC 2 cut(s) 781, 1187
FblI GTMKAC 2 cut(s) 1113, 1221
Fnu4HI GCNGC 8 cut(s) 296, 663, 993, 1292, 1295, 1408, 1464, 1467
FokI GGATG 1 cut(s) 407
Fsp4HI GCNGC 8 cut(s) 296, 663, 993, 1292, 1295, 1408, 1464, 1467
FspBI CTAG 3 cut(s) 335, 1061, 1527
GlaI GCGC 1 cut(s) 1153
GluI GCNGC 8 cut(s) 296, 663, 993, 1292, 1295, 1408, 1464, 1467
HaeIII GGCC 5 cut(s) 407, 696, 1010, 1165, 1345
HapII CCGG 2 cut(s) 349, 354
HgaI GACGC 2 cut(s) 189, 1207
HhaI GCGC 1 cut(s) 1154
Hin1I GRCGYC 1 cut(s) 1218
Hin6I GCGC 1 cut(s) 1152
HinP1I GCGC 1 cut(s) 1152
HinfI GANTC 4 cut(s) 33, 110, 597, 1038
HpaII CCGG 2 cut(s) 349, 354
HphI GGTGA 4 cut(s) 368, 619, 1013, 1447
Hpy166II GTNNAC 4 cut(s) 1090, 1114, 1222, 1357
Hpy188I TCNGA 4 cut(s) 115, 621, 701, 951
Hpy188III TCNNGA 5 cut(s) 881, 1024, 1267, 1496, 1504
Hpy8I GTNNAC 4 cut(s) 1090, 1114, 1222, 1357
HpyAV CCTTC 2 cut(s) 685, 919
HpyCH4III ACNGT 6 cut(s) 530, 772, 790, 934, 958, 1354
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 404, 833, 1076, 1429
HpyF3I CTNAG 8 cut(s) 108, 129, 244, 261, 412, 618, 1243, 1433
Hsp92I GRCGYC 1 cut(s) 1218
HspAI GCGC 1 cut(s) 1152
Kzo9I GATC 6 cut(s) 227, 521, 553, 621, 893, 1498
LmnI GCTCC 6 cut(s) 44, 816, 833, 1149, 1328, 1404
Lsp1109I GCAGC 8 cut(s) 282, 649, 1004, 1278, 1281, 1419, 1450, 1453
LweI GCATC 3 cut(s) 385, 1419, 1446
MaeI CTAG 3 cut(s) 335, 1061, 1527
MalI GATC 6 cut(s) 229, 523, 555, 623, 895, 1500
MboI GATC 6 cut(s) 227, 521, 553, 621, 893, 1498
MboII GAAGA 7 cut(s) 14, 65, 184, 432, 934, 1294, 1402
MfeI CAATTG 1 cut(s) 474
MflI RGATCY 5 cut(s) 227, 553, 621, 893, 1498
MlsI TGGCCA 2 cut(s) 1165, 1345
MluCI AATT 5 cut(s) 184, 379, 474, 876, 1320
MluNI TGGCCA 2 cut(s) 1165, 1345
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 2 cut(s) 118, 1297
Mox20I TGGCCA 2 cut(s) 1165, 1345
MscI TGGCCA 2 cut(s) 1165, 1345
MseI TTAA 5 cut(s) 312, 633, 738, 1425, 1521
MslI CAYNNNNRTG 4 cut(s) 540, 605, 1159, 1286
Msp20I TGGCCA 2 cut(s) 1165, 1345
MspCI CTTAAG 1 cut(s) 1424
MspI CCGG 2 cut(s) 349, 354
MspR9I CCNGG 2 cut(s) 1007, 1014
MunI CAATTG 1 cut(s) 474
MvaI CCWGG 2 cut(s) 1007, 1014
MwoI GCNNNNNNNGC 5 cut(s) 79, 404, 833, 1076, 1429
NcoI CCATGG 1 cut(s) 40
NdeII GATC 6 cut(s) 227, 521, 553, 621, 893, 1498
NlaIV GGNNCC 2 cut(s) 1011, 1330
NmuCI GTSAC 6 cut(s) 103, 201, 356, 364, 1001, 1453
NspI RCATGY 2 cut(s) 568, 823
OliI CACNNNNGTG 1 cut(s) 1159
PaeI GCATGC 1 cut(s) 823
PagI TCATGA 1 cut(s) 1266
PciI ACATGT 1 cut(s) 564
PfeI GAWTC 3 cut(s) 33, 597, 1038
PflFI GACNNNGTC 1 cut(s) 362
PinAI ACCGGT 1 cut(s) 348
PkrI GCNGC 8 cut(s) 297, 664, 994, 1293, 1296, 1409, 1465, 1468
PleI GAGTC 1 cut(s) 118
PpsI GAGTC 1 cut(s) 118
PscI ACATGT 1 cut(s) 564
PsiI TTATAA 1 cut(s) 1512
Psp6I CCWGG 2 cut(s) 1005, 1012
PspEI GGTNACC 2 cut(s) 356, 1001
PspGI CCWGG 2 cut(s) 1005, 1012
PspN4I GGNNCC 2 cut(s) 1011, 1330
PspPI GGNCC 5 cut(s) 281, 351, 445, 1009, 1254
PstI CTGCAG 3 cut(s) 907, 1189, 1471
PsuI RGATCY 5 cut(s) 227, 553, 621, 893, 1498
PsyI GACNNNGTC 1 cut(s) 362
RsaI GTAC 3 cut(s) 452, 581, 1376
RsaNI GTAC 3 cut(s) 451, 580, 1375
RseI CAYNNNNRTG 4 cut(s) 540, 605, 1159, 1286
Rsr2I CGGWCCG 1 cut(s) 351
RsrII CGGWCCG 1 cut(s) 351
SaqAI TTAA 5 cut(s) 312, 633, 738, 1425, 1521
SatI GCNGC 8 cut(s) 296, 663, 993, 1292, 1295, 1408, 1464, 1467
Sau3AI GATC 6 cut(s) 227, 521, 553, 621, 893, 1498
Sau96I GGNCC 5 cut(s) 281, 351, 445, 1009, 1254
ScaI AGTACT 1 cut(s) 452
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 2 cut(s) 1007, 1014
SfaNI GCATC 3 cut(s) 385, 1419, 1446
SfcI CTRYAG 3 cut(s) 903, 1185, 1467
SinI GGWCC 4 cut(s) 281, 351, 445, 1254
SmiMI CAYNNNNRTG 4 cut(s) 540, 605, 1159, 1286
SmlI CTYRAG 2 cut(s) 1424, 1502
SmoI CTYRAG 2 cut(s) 1424, 1502
SphI GCATGC 1 cut(s) 823
Sse9I AATT 5 cut(s) 184, 379, 474, 876, 1320
SspMI CTAG 3 cut(s) 335, 1061, 1527
StyD4I CCNGG 2 cut(s) 1005, 1012
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 6 cut(s) 530, 772, 790, 934, 958, 1354
TasI AATT 5 cut(s) 184, 379, 474, 876, 1320
TatI WGTACW 2 cut(s) 450, 1374
TfiI GAWTC 3 cut(s) 33, 597, 1038
Tru1I TTAA 5 cut(s) 312, 633, 738, 1425, 1521
Tru9I TTAA 5 cut(s) 312, 633, 738, 1425, 1521
TscAI CASTG 2 cut(s) 112, 963
TseFI GTSAC 6 cut(s) 103, 201, 356, 364, 1001, 1453
TseI GCWGC 8 cut(s) 295, 662, 992, 1291, 1294, 1407, 1463, 1466
Tsp45I GTSAC 6 cut(s) 103, 201, 356, 364, 1001, 1453
TspDTI ATGAA 1 cut(s) 935
TspRI CASTG 2 cut(s) 112, 963
Tth111I GACNNNGTC 1 cut(s) 362
Vha464I CTTAAG 1 cut(s) 1424
VpaK11BI GGWCC 4 cut(s) 281, 351, 445, 1254
XagI CCTNNNNNAGG 1 cut(s) 366
XapI RAATTY 3 cut(s) 184, 876, 1320
XceI RCATGY 2 cut(s) 568, 823
XmiI GTMKAC 2 cut(s) 1113, 1221
XspI CTAG 3 cut(s) 335, 1061, 1527
ZrmI AGTACT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.