FvH4_2g01330

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014916
Physical Location & Seq
Forward (+)
1267870 .. 1272050
4181 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g01330.t3

Sequence Viewer

Length: 1539 bp
ATGTTGCCAAGGAGCCGAACTCTTCCAAGCAGAATCCATCATGGAGCTGTGGAGGAGAGGCATGATGTCAGGCACTATTTGCAAGTAGAAGTTCAACCTAAAGTCGGTGAATCTGAAGCTATAAACCCTCAGTCGAACTATTCCAAATGCTTCGATGATGATGGTCGCCTTAAGCGAACAGGAACTTTTTGGACTTCAACATCACATATTATCACTGCAGTTATAGGATCTGGAGTTCTATCGTTAGCATGGGCAATTGCACAGCTTGGTTGGGTTGCAGGACCAACTGTCCTCGCTCTTTTTGCCATTGTCAATCTGTACACTTCCAATCTCCTAGCCATGTGCTATCGAGCAGGTGACCCTGTCACGGGACAGAGAAATTATACATACATGGATGCTGTCAAGGCCAACTTAGGAGGAAGAAGAGTCATGTTATGTGGATTAGTCCAGTACTTGAATTTGTTTGGGGTAGCAATTGGATATACCATTGCATCTTCTGTCAGCATGATGGCAATAAAGAGATCAAACTGCTATCACAAAAGTGGAGGGAAAGATCCATGTCATATGTCAAGCAATGGTTATATGATAACATTTGGAATCATAGAGGTGATATTTTCACAAATCAAAGATTTTAATGAAGTATGGTGGCTTTCTATAGTGGCAGCCATTATGTCTTTCACATACTCTACTGTTGGCCTTGGACTTGGCATTGGCAAAGTTGCAGGAAATGGGGGTTTCAAAGGAAGTCTATTGGGTATTAGCATAGGGACAGTTACTCATTCTGGAACAGTAGTCACATCTACAGAAAAGATGTGGAGGACTATGCAAGCTCTTGGCGCTATTGCATTTGCTTACTCCTATTCTTTGGTCCTCATCGAAATACAGGATACTATAAGATCTCCTCCTGCAGAACATAAGACCATGAAGAAGGCAACTGTATTCAGCATCACTGTCACAACAGTCTTTTATATGCTCTGTGGATGTTTTGGGTACGCGGCGTTTGGTGATCTTGCCCCAGGAAACCTCTTAACTGGCTTTGGATTCTATAACCCCTACTGGCTCCTGGACATTGCAAATGTTGCAATTGTTGTCCACCTAGTAGGTGCATTTCAGGTCTTCTGCCAGCCATTATTTGCATTTGTGGAGAAATGGAGTGCACAAAAATGGCCAAAAAGCGACTTTGTGACAGCCGAGTATGACATACCTATTCCCTTCTATGGTGTCTACCAACTCAACCTGTTTCGCTTGGTATGGAGGACCCTTTTTGTTATATTGACTACCCTCATATCCATGCTCCTACCATTCTTCAATGATGTAGTTGGGATACTTGGAGCCTTTGGATTCTGGCCATTAACTGTTTATTTTCCGGTTGAGATGTACATTGCTCAACAGAAGATTGAGAAATGGAGTAGTAGATGGCTTGGACTTCAGATGCTAAGTATGAGTTGTCTAGTCGTCTCCATAGTTGCTGCAGTTGGCTCTTTCGCCGGTGTAGTTCTAGATCTGAAGTCTTATAAACCCTTCAAAACTACTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

513

Amino Acids

56.61

Weight (kDa)

9.02

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 2 - 356 1.4e-86 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09220 AT5G63850
fragaria_vesca FvH4_2g01330
malus_domestica MD05G1120400.v1.1
prunus_persica Prupe.4G277700_v2.0.a1 Prupe.8G164200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0252251
rosa_laevigata RLG00000015401
rosa_multiflora Rmu_sc0006252.1_g000018 Rmu_sc0009799.1_g000021
rosa_roxburghii Rroxscaffold_7G00216210
rosa_rugosa Rorug05G0506400
rosa_samantha Rh6AG020200 Rh6BG015800 Rh6CG013000
rosa_wichuraiana Rw6G001660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1515
AarI CACCTGC 1 cut(s) 344
Acc36I ACCTGC 1 cut(s) 344
AccI GTMKAC 1 cut(s) 1224
AccII CGCG 1 cut(s) 995
AciI CCGC 1 cut(s) 995
AclWI GGATC 2 cut(s) 235, 548
AcoI YGGCCR 2 cut(s) 1166, 1346
AcsI RAATTY 1 cut(s) 457
AcuI CTGAAG 3 cut(s) 135, 1412, 1526
AfaI GTAC 4 cut(s) 320, 452, 992, 1379
AfiI CCNNNNNNNGG 4 cut(s) 104, 367, 368, 1217
AflII CTTAAG 1 cut(s) 170
AgsI TTSAA 6 cut(s) 95, 198, 457, 739, 1309, 1525
AhdI GACNNNNNGTC 1 cut(s) 287
AjnI CCWGG 2 cut(s) 1015, 1062
AleI CACNNNNGTG 1 cut(s) 540
AloI GAACNNNNNNTCC 2 cut(s) 219, 251
AluBI AGCT 4 cut(s) 47, 119, 265, 830
AluI AGCT 4 cut(s) 47, 119, 265, 830
Alw21I GWGCWC 1 cut(s) 1159
Alw26I GTCTC 1 cut(s) 1462
Alw44I GTGCAC 1 cut(s) 1155
AlwI GGATC 2 cut(s) 235, 548
AoxI GGCC 4 cut(s) 405, 694, 1166, 1346
ApaLI GTGCAC 1 cut(s) 1155
ApeKI GCWGC 2 cut(s) 662, 1469
ApoI RAATTY 1 cut(s) 457
AspLEI GCGC 1 cut(s) 839
AspS9I GGNCC 3 cut(s) 281, 868, 1257
AsuHPI GGTGA 4 cut(s) 119, 368, 619, 1016
AvaII GGWCC 3 cut(s) 281, 868, 1257
BaeGI GKGCMC 1 cut(s) 1159
BalI TGGCCA 2 cut(s) 1168, 1348
BbsI GAAGAC 1 cut(s) 1108
Bbv12I GWGCWC 1 cut(s) 1159
BbvI GCAGC 2 cut(s) 674, 1456
BccI CCATC 4 cut(s) 45, 155, 502, 1410
BciT130I CCWGG 2 cut(s) 1017, 1064
BciVI GTATCC 2 cut(s) 880, 1317
BcoDI GTCTC 1 cut(s) 1462
BfaI CTAG 4 cut(s) 335, 1097, 1451, 1499
BfmI CTRYAG 5 cut(s) 216, 654, 801, 906, 1470
BfoI RGCGCY 1 cut(s) 840
BfrI CTTAAG 1 cut(s) 170
BfuAI ACCTGC 1 cut(s) 344
BfuI GTATCC 2 cut(s) 880, 1317
BglII AGATCT 2 cut(s) 896, 1501
BisI GCNGC 3 cut(s) 663, 996, 1470
BlsI GCNGC 3 cut(s) 664, 997, 1471
BmcAI AGTACT 1 cut(s) 452
Bme1390I CCNGG 2 cut(s) 1017, 1064
Bme18I GGWCC 3 cut(s) 281, 868, 1257
BmeRI GACNNNNNGTC 1 cut(s) 287
BmgT120I GGNCC 3 cut(s) 281, 868, 1257
BmiI GGNNCC 4 cut(s) 14, 1061, 1259, 1333
BmrFI CCNGG 2 cut(s) 1017, 1064
BmsI GCATC 4 cut(s) 385, 500, 954, 1422
BpiI GAAGAC 1 cut(s) 1108
BplI GAGNNNNNCTC 1 cut(s) 36
BpmI CTGGAG 1 cut(s) 252
BsaJI CCNNGG 3 cut(s) 8, 697, 1015
BsaWI WCCGGW 1 cut(s) 1366
Bsc4I CCNNNNNNNGG 4 cut(s) 104, 367, 368, 1217
Bse118I RCCGGY 1 cut(s) 1487
Bse1I ACTGG 3 cut(s) 448, 1036, 1061
Bse3DI GCAATG 4 cut(s) 486, 580, 1068, 1380
BseBI CCWGG 2 cut(s) 1017, 1064
BseDI CCNNGG 3 cut(s) 8, 697, 1015
BseGI GGATG 2 cut(s) 400, 986
BseLI CCNNNNNNNGG 4 cut(s) 104, 367, 368, 1217
BseMI GCAATG 4 cut(s) 486, 580, 1068, 1380
BseMII CTCAG 1 cut(s) 143
BseNI ACTGG 3 cut(s) 448, 1036, 1061
BseRI GAGGAG 2 cut(s) 68, 891
BseSI GKGCMC 1 cut(s) 1159
BseXI GCAGC 2 cut(s) 674, 1456
Bsh1236I CGCG 1 cut(s) 995
BshFI GGCC 4 cut(s) 407, 696, 1168, 1348
BsiHKAI GWGCWC 1 cut(s) 1159
BsiSI CCGG 2 cut(s) 1367, 1488
BslFI GGGAC 2 cut(s) 384, 781
BslI CCNNNNNNNGG 4 cut(s) 104, 367, 368, 1217
BsmAI GTCTC 1 cut(s) 1462
BsmBI CGTCTC 1 cut(s) 1462
BsmFI GGGAC 2 cut(s) 384, 781
BsnI GGCC 4 cut(s) 407, 696, 1168, 1348
Bsp1286I GDGCHC 1 cut(s) 1159
Bsp1407I TGTACA 2 cut(s) 318, 1377
Bsp143I GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
BspACI CCGC 1 cut(s) 995
BspANI GGCC 4 cut(s) 407, 696, 1168, 1348
BspCNI CTCAG 1 cut(s) 142
BspFNI CGCG 1 cut(s) 995
BspLI GGNNCC 4 cut(s) 14, 1061, 1259, 1333
BspMAI CTGCAG 3 cut(s) 220, 910, 1474
BspMI ACCTGC 1 cut(s) 344
BspPI GGATC 2 cut(s) 235, 548
BspTI CTTAAG 1 cut(s) 170
BsrDI GCAATG 4 cut(s) 486, 580, 1068, 1380
BsrFI RCCGGY 1 cut(s) 1487
BsrGI TGTACA 2 cut(s) 318, 1377
BsrI ACTGG 3 cut(s) 448, 1036, 1061
BssAI RCCGGY 1 cut(s) 1487
BssECI CCNNGG 3 cut(s) 8, 697, 1015
BssMI GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
BssT1I CCWWGG 2 cut(s) 8, 697
Bst2UI CCWGG 2 cut(s) 1017, 1064
Bst4CI ACNGT 8 cut(s) 289, 691, 772, 790, 937, 952, 961, 1357
Bst6I CTCTTC 2 cut(s) 27, 418
BstAFI CTTAAG 1 cut(s) 170
BstAPI GCANNNNNTGC 2 cut(s) 79, 1079
BstAUI TGTACA 2 cut(s) 318, 1377
BstC8I GCNNGC 2 cut(s) 828, 1124
BstDEI CTNAG 3 cut(s) 129, 412, 1436
BstEII GGTNACC 1 cut(s) 356
BstF5I GGATG 2 cut(s) 400, 986
BstFNI CGCG 1 cut(s) 995
BstH2I RGCGCY 1 cut(s) 840
BstHHI GCGC 1 cut(s) 839
BstKTI GATC 6 cut(s) 230, 524, 556, 899, 1009, 1504
BstMAI GTCTC 1 cut(s) 1462
BstMBI GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
BstMWI GCNNNNNNNGC 5 cut(s) 79, 302, 404, 836, 1079
BstNI CCWGG 2 cut(s) 1017, 1064
BstPI GGTNACC 1 cut(s) 356
BstSCI CCNGG 2 cut(s) 1015, 1062
BstSFI CTRYAG 5 cut(s) 216, 654, 801, 906, 1470
BstSLI GKGCMC 1 cut(s) 1159
BstUI CGCG 1 cut(s) 995
BstV1I GCAGC 2 cut(s) 674, 1456
BstV2I GAAGAC 1 cut(s) 1108
BstX2I RGATCY 4 cut(s) 227, 553, 896, 1501
BstYI RGATCY 4 cut(s) 227, 553, 896, 1501
BsuI GTATCC 2 cut(s) 880, 1317
BsuRI GGCC 4 cut(s) 407, 696, 1168, 1348
BtsCI GGATG 2 cut(s) 400, 986
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 2 cut(s) 213, 948
BveI ACCTGC 1 cut(s) 344
Cac8I GCNNGC 2 cut(s) 828, 1124
CfoI GCGC 1 cut(s) 839
Cfr10I RCCGGY 1 cut(s) 1487
Cfr13I GGNCC 3 cut(s) 281, 868, 1257
Csp6I GTAC 4 cut(s) 319, 451, 991, 1378
CviQI GTAC 4 cut(s) 319, 451, 991, 1378
DdeI CTNAG 3 cut(s) 129, 412, 1436
DpnI GATC 6 cut(s) 229, 523, 555, 898, 1008, 1503
DpnII GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
DriI GACNNNNNGTC 1 cut(s) 287
EaeI YGGCCR 2 cut(s) 1166, 1346
Eam1104I CTCTTC 2 cut(s) 27, 418
Eam1105I GACNNNNNGTC 1 cut(s) 287
EarI CTCTTC 2 cut(s) 27, 418
Eco130I CCWWGG 2 cut(s) 8, 697
Eco47I GGWCC 3 cut(s) 281, 868, 1257
Eco57I CTGAAG 3 cut(s) 135, 1412, 1526
Eco91I GGTNACC 1 cut(s) 356
EcoO109I RGGNCCY 1 cut(s) 1257
EcoO65I GGTNACC 1 cut(s) 356
EcoRII CCWGG 2 cut(s) 1015, 1062
EcoT14I CCWWGG 2 cut(s) 8, 697
ErhI CCWWGG 2 cut(s) 8, 697
Esp3I CGTCTC 1 cut(s) 1462
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FaqI GGGAC 2 cut(s) 384, 781
FauNDI CATATG 1 cut(s) 564
FblI GTMKAC 1 cut(s) 1224
Fnu4HI GCNGC 3 cut(s) 663, 996, 1470
FokI GGATG 2 cut(s) 407, 993
Fsp4HI GCNGC 3 cut(s) 663, 996, 1470
FspBI CTAG 4 cut(s) 335, 1097, 1451, 1499
GlaI GCGC 1 cut(s) 838
GluI GCNGC 3 cut(s) 663, 996, 1470
GsuI CTGGAG 1 cut(s) 252
HaeII RGCGCY 1 cut(s) 840
HaeIII GGCC 4 cut(s) 407, 696, 1168, 1348
HapII CCGG 2 cut(s) 1367, 1488
HhaI GCGC 1 cut(s) 839
Hin6I GCGC 1 cut(s) 837
HinP1I GCGC 1 cut(s) 837
HinfI GANTC 6 cut(s) 33, 110, 426, 597, 1041, 1341
HpaII CCGG 2 cut(s) 1367, 1488
HphI GGTGA 4 cut(s) 119, 368, 619, 1016
Hpy166II GTNNAC 4 cut(s) 321, 1093, 1157, 1225
Hpy188I TCNGA 3 cut(s) 115, 1431, 1506
Hpy188III TCNNGA 3 cut(s) 231, 783, 1499
Hpy8I GTNNAC 4 cut(s) 321, 1093, 1157, 1225
HpyAV CCTTC 3 cut(s) 922, 1222, 1531
HpyCH4III ACNGT 8 cut(s) 289, 691, 772, 790, 937, 952, 961, 1357
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 302, 404, 836, 1079
HpyF3I CTNAG 3 cut(s) 129, 412, 1436
HspAI GCGC 1 cut(s) 837
Kzo9I GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
LmnI GCTCC 5 cut(s) 12, 44, 1065, 1299, 1331
Lsp1109I GCAGC 2 cut(s) 674, 1456
LweI GCATC 4 cut(s) 385, 500, 954, 1422
MaeI CTAG 4 cut(s) 335, 1097, 1451, 1499
MaeIII GTNAC 6 cut(s) 356, 364, 772, 793, 952, 1183
MalI GATC 6 cut(s) 229, 523, 555, 898, 1008, 1503
MboI GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
MboII GAAGA 8 cut(s) 14, 432, 435, 486, 937, 1108, 1297, 1405
MfeI CAATTG 3 cut(s) 255, 474, 1083
MflI RGATCY 4 cut(s) 227, 553, 896, 1501
MhlI GDGCHC 1 cut(s) 1159
MlsI TGGCCA 2 cut(s) 1168, 1348
MluCI AATT 5 cut(s) 255, 379, 457, 474, 1083
MluNI TGGCCA 2 cut(s) 1168, 1348
MlyI GAGTC 1 cut(s) 435
Mox20I TGGCCA 2 cut(s) 1168, 1348
MscI TGGCCA 2 cut(s) 1168, 1348
MseI TTAA 4 cut(s) 171, 633, 1028, 1352
MslI CAYNNNNRTG 4 cut(s) 540, 605, 1162, 1289
Msp20I TGGCCA 2 cut(s) 1168, 1348
MspCI CTTAAG 1 cut(s) 170
MspI CCGG 2 cut(s) 1367, 1488
MspR9I CCNGG 2 cut(s) 1017, 1064
MunI CAATTG 3 cut(s) 255, 474, 1083
MvaI CCWGG 2 cut(s) 1017, 1064
MvnI CGCG 1 cut(s) 995
MwoI GCNNNNNNNGC 5 cut(s) 79, 302, 404, 836, 1079
NdeI CATATG 1 cut(s) 564
NdeII GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
NlaIV GGNNCC 4 cut(s) 14, 1061, 1259, 1333
NmeAIII GCCGAG 1 cut(s) 1216
NmuCI GTSAC 5 cut(s) 356, 364, 793, 952, 1183
OliI CACNNNNGTG 1 cut(s) 540
PaqCI CACCTGC 1 cut(s) 344
PcsI WCGNNNNNNNCGW 1 cut(s) 172
PfeI GAWTC 5 cut(s) 33, 110, 597, 1041, 1341
PflFI GACNNNGTC 1 cut(s) 362
PfoI TCCNGGA 1 cut(s) 1062
PkrI GCNGC 3 cut(s) 664, 997, 1471
PleI GAGTC 1 cut(s) 434
PpsI GAGTC 1 cut(s) 434
PpuMI RGGWCCY 1 cut(s) 1257
PsiI TTATAA 1 cut(s) 1515
Psp5II RGGWCCY 1 cut(s) 1257
Psp6I CCWGG 2 cut(s) 1015, 1062
PspEI GGTNACC 1 cut(s) 356
PspGI CCWGG 2 cut(s) 1015, 1062
PspN4I GGNNCC 4 cut(s) 14, 1061, 1259, 1333
PspPI GGNCC 3 cut(s) 281, 868, 1257
PspPPI RGGWCCY 1 cut(s) 1257
PstI CTGCAG 3 cut(s) 220, 910, 1474
PsuI RGATCY 4 cut(s) 227, 553, 896, 1501
PsyI GACNNNGTC 1 cut(s) 362
RsaI GTAC 4 cut(s) 320, 452, 992, 1379
RsaNI GTAC 4 cut(s) 319, 451, 991, 1378
RseI CAYNNNNRTG 4 cut(s) 540, 605, 1162, 1289
SaqAI TTAA 4 cut(s) 171, 633, 1028, 1352
SatI GCNGC 3 cut(s) 663, 996, 1470
Sau3AI GATC 6 cut(s) 227, 521, 553, 896, 1006, 1501
Sau96I GGNCC 3 cut(s) 281, 868, 1257
ScaI AGTACT 1 cut(s) 452
SchI GAGTC 1 cut(s) 435
ScrFI CCNGG 2 cut(s) 1017, 1064
SduI GDGCHC 1 cut(s) 1159
SfaNI GCATC 4 cut(s) 385, 500, 954, 1422
SfcI CTRYAG 5 cut(s) 216, 654, 801, 906, 1470
SgrAI CRCCGGYG 1 cut(s) 1487
SinI GGWCC 3 cut(s) 281, 868, 1257
SmiMI CAYNNNNRTG 4 cut(s) 540, 605, 1162, 1289
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 5 cut(s) 255, 379, 457, 474, 1083
SsiI CCGC 1 cut(s) 995
SspMI CTAG 4 cut(s) 335, 1097, 1451, 1499
StyD4I CCNGG 2 cut(s) 1015, 1062
StyI CCWWGG 2 cut(s) 8, 697
TaaI ACNGT 8 cut(s) 289, 691, 772, 790, 937, 952, 961, 1357
TaqI TCGA 4 cut(s) 134, 153, 349, 876
TasI AATT 5 cut(s) 255, 379, 457, 474, 1083
TatI WGTACW 3 cut(s) 318, 450, 1377
TauI GCSGC 1 cut(s) 998
TfiI GAWTC 5 cut(s) 33, 110, 597, 1041, 1341
Tru1I TTAA 4 cut(s) 171, 633, 1028, 1352
Tru9I TTAA 4 cut(s) 171, 633, 1028, 1352
TscAI CASTG 2 cut(s) 220, 955
TseFI GTSAC 5 cut(s) 356, 364, 793, 952, 1183
TseI GCWGC 2 cut(s) 662, 1469
Tsp45I GTSAC 5 cut(s) 356, 364, 793, 952, 1183
TspDTI ATGAA 2 cut(s) 651, 938
TspRI CASTG 2 cut(s) 220, 955
Tth111I GACNNNGTC 1 cut(s) 362
Vha464I CTTAAG 1 cut(s) 170
VneI GTGCAC 1 cut(s) 1155
VpaK11BI GGWCC 3 cut(s) 281, 868, 1257
XapI RAATTY 1 cut(s) 457
XbaI TCTAGA 1 cut(s) 1498
XmiI GTMKAC 1 cut(s) 1224
XspI CTAG 4 cut(s) 335, 1097, 1451, 1499
ZrmI AGTACT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.