AT5G64850

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008656
Physical Location & Seq
Forward (+)
25921402 .. 25923418
2017 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64850.1

Sequence Viewer

Length: 345 bp
ATGGAAGATCGAAAAGAGAACAAGAACTCGCCATGGCTATCAGTACCACAGTTTGGAGATTGGGATCAAAAAGGAGGATCAATTGATTACTCAATGGATTTCTCAAAGATCAGAGAGATGAGGAAACTCAACAAGAGAGATGCTTCTCGAGCCAGTCTTGGCAACGAGGAAGAACTCATTAACCCTTTTCATGATCAGCCTCCTGTTGATACTGCCAAGCCTAAGAAGCTAACTACGGTTCACAGTGACAACAACAACCGCAATGAGTTCTCTCACCATCACCCACACTCTCCTTCTAGGAGGAGAAGAATCTTCAGCTGCTTCAACTGTTGCGTTAAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AvrRpt-cleavage

Protein Analysis

114

Amino Acids

13.32

Weight (kDa)

9.12

Isoelectric Point (pI)

56.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AvrRpt-cleavage PF05627 12 - 42 1.6e-08 Cleavage site for pathogenic type III effector avirulence factor Avr
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 53
AciI CCGC 1 cut(s) 259
AclWI GGATC 2 cut(s) 72, 85
AcuI CTGAAG 1 cut(s) 298
AfaI GTAC 1 cut(s) 45
AfiI CCNNNNNNNGG 1 cut(s) 53
AgsI TTSAA 1 cut(s) 325
AluBI AGCT 3 cut(s) 229, 318, 341
AluI AGCT 3 cut(s) 229, 318, 341
AlwI GGATC 2 cut(s) 72, 85
Ama87I CYCGRG 1 cut(s) 147
ApeKI GCWGC 1 cut(s) 318
AsuHPI GGTGA 2 cut(s) 266, 272
AvaI CYCGRG 1 cut(s) 147
BbvI GCAGC 1 cut(s) 305
BccI CCATC 1 cut(s) 285
BclI TGATCA 1 cut(s) 193
BfaI CTAG 1 cut(s) 297
BisI GCNGC 1 cut(s) 319
BlsI GCNGC 1 cut(s) 320
BmeT110I CYCGRG 1 cut(s) 147
BmsI GCATC 1 cut(s) 130
BsaBI GATNNNNATC 1 cut(s) 63
BsaJI CCNNGG 1 cut(s) 32
Bsc4I CCNNNNNNNGG 1 cut(s) 53
Bse1I ACTGG 1 cut(s) 153
Bse3DI GCAATG 1 cut(s) 268
Bse8I GATNNNNATC 1 cut(s) 63
BseDI CCNNGG 1 cut(s) 32
BseJI GATNNNNATC 1 cut(s) 63
BseLI CCNNNNNNNGG 1 cut(s) 53
BseMI GCAATG 1 cut(s) 268
BseNI ACTGG 1 cut(s) 153
BseRI GAGGAG 1 cut(s) 316
BseXI GCAGC 1 cut(s) 305
BsiHKCI CYCGRG 1 cut(s) 147
BslI CCNNNNNNNGG 1 cut(s) 53
BsoBI CYCGRG 1 cut(s) 147
Bsp143I GATC 5 cut(s) 7, 64, 77, 108, 193
Bsp19I CCATGG 1 cut(s) 32
BspACI CCGC 1 cut(s) 259
BspHI TCATGA 1 cut(s) 190
BspPI GGATC 2 cut(s) 72, 85
BsrDI GCAATG 1 cut(s) 268
BsrI ACTGG 1 cut(s) 153
BssECI CCNNGG 1 cut(s) 32
BssMI GATC 5 cut(s) 7, 64, 77, 108, 193
BssT1I CCWWGG 1 cut(s) 32
Bst4CI ACNGT 4 cut(s) 51, 238, 245, 329
BstDEI CTNAG 1 cut(s) 222
BstDSI CCRYGG 1 cut(s) 32
BstKTI GATC 5 cut(s) 10, 67, 80, 111, 196
BstMBI GATC 5 cut(s) 7, 64, 77, 108, 193
BstMWI GCNNNNNNNGC 2 cut(s) 149, 226
BstV1I GCAGC 1 cut(s) 305
BtgI CCRYGG 1 cut(s) 32
BtsIMutI CAGTG 1 cut(s) 250
CciI TCATGA 1 cut(s) 190
Csp6I GTAC 1 cut(s) 44
CviAII CATG 2 cut(s) 33, 191
CviJI RGCY 7 cut(s) 37, 152, 199, 220, 229, 318, 341
CviKI_1 RGCY 7 cut(s) 37, 152, 199, 220, 229, 318, 341
CviQI GTAC 1 cut(s) 44
DdeI CTNAG 1 cut(s) 222
DpnI GATC 5 cut(s) 9, 66, 79, 110, 195
DpnII GATC 5 cut(s) 7, 64, 77, 108, 193
Eco130I CCWWGG 1 cut(s) 32
Eco57I CTGAAG 1 cut(s) 298
Eco88I CYCGRG 1 cut(s) 147
EcoT14I CCWWGG 1 cut(s) 32
ErhI CCWWGG 1 cut(s) 32
FaeI CATG 2 cut(s) 36, 194
FaiI YATR 2 cut(s) 34, 192
FatI CATG 2 cut(s) 32, 190
FbaI TGATCA 1 cut(s) 193
Fnu4HI GCNGC 1 cut(s) 319
Fsp4HI GCNGC 1 cut(s) 319
FspBI CTAG 1 cut(s) 297
GluI GCNGC 1 cut(s) 319
Hin1II CATG 2 cut(s) 36, 194
HindIII AAGCTT 1 cut(s) 339
HinfI GANTC 1 cut(s) 309
HphI GGTGA 2 cut(s) 266, 272
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 1 cut(s) 113
Hpy188III TCNNGA 2 cut(s) 147, 191
Hpy8I GTNNAC 1 cut(s) 241
HpyAV CCTTC 1 cut(s) 303
HpyCH4III ACNGT 4 cut(s) 51, 238, 245, 329
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 226
HpyF3I CTNAG 1 cut(s) 222
Hsp92II CATG 2 cut(s) 36, 194
Ksp22I TGATCA 1 cut(s) 193
Kzo9I GATC 5 cut(s) 7, 64, 77, 108, 193
LpnPI CCDG 2 cut(s) 166, 216
Lsp1109I GCAGC 1 cut(s) 305
LweI GCATC 1 cut(s) 130
MaeI CTAG 1 cut(s) 297
MaeIII GTNAC 1 cut(s) 245
MalI GATC 5 cut(s) 9, 66, 79, 110, 195
MboI GATC 5 cut(s) 7, 64, 77, 108, 193
MboII GAAGA 4 cut(s) 17, 182, 304, 318
MfeI CAATTG 1 cut(s) 81
MluCI AATT 1 cut(s) 81
MnlI CCTC 5 cut(s) 68, 114, 160, 210, 294
MseI TTAA 3 cut(s) 180, 336, 343
MspA1I CMGCKG 1 cut(s) 318
MunI CAATTG 1 cut(s) 81
MwoI GCNNNNNNNGC 2 cut(s) 149, 226
NcoI CCATGG 1 cut(s) 32
NdeII GATC 5 cut(s) 7, 64, 77, 108, 193
NlaIII CATG 2 cut(s) 36, 194
NmuCI GTSAC 1 cut(s) 245
PaeR7I CTCGAG 1 cut(s) 147
PagI TCATGA 1 cut(s) 190
PfeI GAWTC 1 cut(s) 309
PflMI CCANNNNNTGG 1 cut(s) 53
PkrI GCNGC 1 cut(s) 320
PvuII CAGCTG 1 cut(s) 318
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
SaqAI TTAA 3 cut(s) 180, 336, 343
SatI GCNGC 1 cut(s) 319
Sau3AI GATC 5 cut(s) 7, 64, 77, 108, 193
SetI ASST 3 cut(s) 231, 320, 343
SfaNI GCATC 1 cut(s) 130
Sfr274I CTCGAG 1 cut(s) 147
SlaI CTCGAG 1 cut(s) 147
SmlI CTYRAG 1 cut(s) 147
SmoI CTYRAG 1 cut(s) 147
Sse9I AATT 1 cut(s) 81
SsiI CCGC 1 cut(s) 259
SspMI CTAG 1 cut(s) 297
StyI CCWWGG 1 cut(s) 32
TaaI ACNGT 4 cut(s) 51, 238, 245, 329
TaqI TCGA 2 cut(s) 10, 148
TasI AATT 1 cut(s) 81
TfiI GAWTC 1 cut(s) 309
Tru1I TTAA 3 cut(s) 180, 336, 343
Tru9I TTAA 3 cut(s) 180, 336, 343
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 1 cut(s) 318
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 1 cut(s) 179
TspRI CASTG 1 cut(s) 250
Van91I CCANNNNNTGG 1 cut(s) 53
XhoI CTCGAG 1 cut(s) 147
XspI CTAG 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.