MD01G1020100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008656
Physical Location & Seq
Forward (+)
8286502 .. 8290386
3885 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1020100.v1.1.491

Sequence Viewer

Length: 297 bp
ATGGAGGATCGTAAGGAGAAAAATGCACCATGGTTATCAGTGCCACAATTCGGGGACTGGGATCAGAAGGGGCAAGTGCCAGACTATTCTCTTGATTTCTCAAAAATAAGGGAAGTGAGGAAGCAAAACAAGAGAGATGTTTCGAGAGCCAGTCTTGGAAATGAAGAAGAGCTCATTGCCTCAAACACTGCTAAAGTGGGTGCTGTTCACAATGAACCCCATTACCATCAGACCCCCCACTCTCCAACTGCTCGACGGAGTATTTTCAGCTACTTCAACTGTTGTGTGAAGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AvrRpt-cleavage

Protein Analysis

99

Amino Acids

11.27

Weight (kDa)

8.62

Isoelectric Point (pI)

51.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AvrRpt-cleavage PF05627 11 - 41 4.6e-08 Cleavage site for pathogenic type III effector avirulence factor Avr
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 15, 69
AfiI CCNNNNNNNGG 1 cut(s) 50
AgsI TTSAA 1 cut(s) 277
AluBI AGCT 2 cut(s) 172, 270
AluI AGCT 2 cut(s) 172, 270
Alw21I GWGCWC 1 cut(s) 174
AlwI GGATC 2 cut(s) 15, 69
AoxI GGCC 1 cut(s) 291
BanII GRGCYC 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 174
BccI CCATC 1 cut(s) 234
BmrI ACTGGG 1 cut(s) 67
BmuI ACTGGG 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 29
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse1I ACTGG 2 cut(s) 62, 150
Bse3DI GCAATG 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 29
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMI GCAATG 1 cut(s) 174
BseNI ACTGG 2 cut(s) 62, 150
BshFI GGCC 1 cut(s) 293
BsiHKAI GWGCWC 1 cut(s) 174
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 1 cut(s) 50
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 2 cut(s) 7, 61
Bsp19I CCATGG 1 cut(s) 29
BspANI GGCC 1 cut(s) 293
BspPI GGATC 2 cut(s) 15, 69
BspQI GCTCTTC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 174
BsrI ACTGG 2 cut(s) 62, 150
BssECI CCNNGG 1 cut(s) 29
BssMI GATC 2 cut(s) 7, 61
BssT1I CCWWGG 1 cut(s) 29
Bst4CI ACNGT 1 cut(s) 281
Bst6I CTCTTC 1 cut(s) 162
BstDSI CCRYGG 1 cut(s) 29
BstKTI GATC 2 cut(s) 10, 64
BstMBI GATC 2 cut(s) 7, 61
BsuRI GGCC 1 cut(s) 293
BtgI CCRYGG 1 cut(s) 29
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 2 cut(s) 45, 186
CviAII CATG 1 cut(s) 30
CviJI RGCY 4 cut(s) 149, 172, 270, 293
CviKI_1 RGCY 4 cut(s) 149, 172, 270, 293
DpnI GATC 2 cut(s) 9, 63
DpnII GATC 2 cut(s) 7, 61
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 29
Eco147I AGGCCT 1 cut(s) 293
Eco24I GRGCYC 1 cut(s) 174
Eco53kI GAGCTC 1 cut(s) 172
EcoICRI GAGCTC 1 cut(s) 172
EcoT14I CCWWGG 1 cut(s) 29
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 29
FaeI CATG 1 cut(s) 33
FaiI YATR 1 cut(s) 31
FaqI GGGAC 1 cut(s) 68
FatI CATG 1 cut(s) 29
FriOI GRGCYC 1 cut(s) 174
HaeIII GGCC 1 cut(s) 293
Hin1II CATG 1 cut(s) 33
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 2 cut(s) 66, 231
Hpy188III TCNNGA 2 cut(s) 92, 144
Hpy8I GTNNAC 1 cut(s) 208
Hpy99I CGWCG 1 cut(s) 258
HpyAV CCTTC 2 cut(s) 61, 283
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4V TGCA 1 cut(s) 26
Hsp92II CATG 1 cut(s) 33
Kzo9I GATC 2 cut(s) 7, 61
LguI GCTCTTC 1 cut(s) 162
LpnPI CCDG 3 cut(s) 43, 93, 163
MalI GATC 2 cut(s) 9, 63
MboI GATC 2 cut(s) 7, 61
MboII GAAGA 2 cut(s) 176, 179
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 269
MnlI CCTC 2 cut(s) 111, 190
NcoI CCATGG 1 cut(s) 29
NdeII GATC 2 cut(s) 7, 61
NlaIII CATG 1 cut(s) 33
PceI AGGCCT 1 cut(s) 293
PciSI GCTCTTC 1 cut(s) 162
Psp124BI GAGCTC 1 cut(s) 174
SacI GAGCTC 1 cut(s) 174
SapI GCTCTTC 1 cut(s) 162
Sau3AI GATC 2 cut(s) 7, 61
SduI GDGCHC 1 cut(s) 174
SetI ASST 2 cut(s) 174, 272
Sse9I AATT 1 cut(s) 47
SseBI AGGCCT 1 cut(s) 293
SstI GAGCTC 1 cut(s) 174
StuI AGGCCT 1 cut(s) 293
StyI CCWWGG 1 cut(s) 29
TaaI ACNGT 1 cut(s) 281
TaqI TCGA 2 cut(s) 143, 253
TasI AATT 1 cut(s) 47
TscAI CASTG 2 cut(s) 45, 193
TspDTI ATGAA 2 cut(s) 177, 228
TspGWI ACGGA 1 cut(s) 271
TspRI CASTG 2 cut(s) 45, 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.