AT5G65890

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007417
Physical Location & Seq
Forward (+)
26354853 .. 26357905
3053 bp
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UTR
Exon/CDS
Intron
AT5G65890.1

Sequence Viewer

Length: 1434 bp
ATGATGGAAATAGCTTATCAGCCTCGGATTGATTCCGAGATTGAATCACTCGTCGAGAGAATCAATCCTCCAAGGGTTTGTGTAGACAACGACTCTGACCCAGAGTGCACACTTATTAAGGTAGATAGCGCGAACAAGTACGGGATATTACTGGACATGGTTCAAGTGTTAGCCGATCTTGATCTCGTTATCTCCAAATGTTACATCTCATCTGATGGCGAATGGTTCATGGATGTGTTTCACGTGACAGACCAACTCGGGAATAAGCTCACCGACCGAAGCCTTATTCTCTACATTCAACAGGCAATATGTTCTAGTAGAACGGGAGGTATAACAAAGGAGATGCAGAGTAATCTAAAACGGGAGGTGCAACAGCGCCACGTGTCAACAGAACACACGGCCTTTGAGATAACCGGAATCAATAGACCGGGTCTACTATCCGAAATATCTGCCGTACTTTCCGACATTGGATGTCACGTGACGGCTGCTGTTGCATGGACCCACCACGAGCGAGCGGCTATGGTGATTTATCTAGAAGACGGGTTTAACGGTGGGCCCATTATCGACCCAATAAGAAAGGCCCAAGTAAAGGATCATCTGGATACAGTCATGGAGGCCCATCACATAGTTGGCGATGTGTCTCATGTCGTCGTGAGGGTCGTCGAGGCTAAAGGAGTTCCGGTCGGGTGGGCTCACACTGAGCGACGACTTCACGAGCTAATGTACGGAGAAGGAGATTACGAGAATTGCTTCGACTGCGATTGTTTCGGAGACAGGTGCGATGCTTTGTGGAGAGGAAGATGCGAGAGGATACACGTGACAATCGAAGCATGTAATGGCTATTCAATGGTGAATGTTAAGTGTAGAGACAGACCAAAACTACTTTTCGACACGGTGTGTGCTTTGAAAGAGTTACAATTCGTTGTGTTTCACGCCGTCGCCGGAGCTAAAGGCTCCACTGCAGAGCAAGAATATTTCATAAGAAAGAAGAACGGGGGCACGTTAGAAACGGAAGGGCAAAGAGAGAGACTAAGGCATTGTTTGGTTGCTGCGATTTCGAGACGTGCTTCACAAGGATTGAAACTCGAGATTCGGACAGAGAACAAGATGGGTTTGTTGTCGGATGTGACTAGAGTTGTGAGAGAAAACGGTTTATCGATAACGAGAGCTGAGATGTGCACGCAAGGTGAGATTGCGGTTGGCTCGTTTTATGTTACGGATGTGAATGGTGGTGAGACGGGTCCTAGTGAGGTTGAAGCGGTTGTACGAGAATTGGGAGGAGCGGTTGTGTCAGCGGTTAAAGGGGTTGGAATGATGCCGAGGAGGATTGGTTCGACGAGTGATAGTGTGGAGCAGGACAAAGCTAAGTCGTCTATTGGGAGAATGTTTTGGTCTAAATTAGAGAGGTTGTCAACGTCTATAAGATCGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0016597 GO:0031406 GO:0036094 GO:0043167 GO:0043168 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT ACT_6

Protein Analysis

477

Amino Acids

52.98

Weight (kDa)

5.81

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 362 - 426 4.6e-08 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 515, 1283
AccI GTMKAC 2 cut(s) 84, 433
AccII CGCG 1 cut(s) 131
AciI CCGC 5 cut(s) 515, 1196, 1259, 1283, 1295
AclWI GGATC 1 cut(s) 600
AcvI CACGTG 4 cut(s) 244, 382, 478, 817
AdeI CACNNNGTG 1 cut(s) 897
AfaI GTAC 4 cut(s) 140, 456, 725, 1266
AfiI CCNNNNNNNGG 1 cut(s) 589
AflIII ACRYGT 2 cut(s) 381, 814
AgsI TTSAA 7 cut(s) 44, 164, 299, 846, 907, 1081, 1256
AjiI CACGTC 1 cut(s) 1064
AloI GAACNNNNNNTCC 2 cut(s) 1315, 1347
AluBI AGCT 6 cut(s) 14, 268, 718, 947, 1169, 1364
AluI AGCT 6 cut(s) 14, 268, 718, 947, 1169, 1364
Alw21I GWGCWC 2 cut(s) 110, 1181
Alw26I GTCTC 6 cut(s) 645, 765, 861, 1021, 1054, 1229
Alw44I GTGCAC 2 cut(s) 106, 1177
AlwI GGATC 1 cut(s) 600
Ama87I CYCGRG 2 cut(s) 257, 1085
AoxI GGCC 4 cut(s) 399, 554, 579, 615
ApaI GGGCCC 1 cut(s) 558
ApaLI GTGCAC 2 cut(s) 106, 1177
ApeKI GCWGC 2 cut(s) 485, 1049
Asp700I GAANNNNTTC 1 cut(s) 749
AspLEI GCGC 2 cut(s) 131, 378
AspS9I GGNCC 6 cut(s) 498, 554, 555, 580, 616, 1241
AsuC2I CCSGG 1 cut(s) 429
AsuHPI GGTGA 5 cut(s) 262, 535, 862, 1199, 1244
AvaI CYCGRG 2 cut(s) 257, 1085
AvaII GGWCC 2 cut(s) 498, 1241
BaeGI GKGCMC 4 cut(s) 110, 558, 1001, 1181
BanII GRGCYC 2 cut(s) 558, 694
BarI GAAGNNNNNNTAC 2 cut(s) 1248, 1280
BauI CACGAG 2 cut(s) 506, 713
BbrPI CACGTG 4 cut(s) 244, 382, 478, 817
BbsI GAAGAC 1 cut(s) 543
Bbv12I GWGCWC 2 cut(s) 110, 1181
BbvI GCAGC 2 cut(s) 472, 1036
BccI CCATC 3 cut(s) 209, 627, 1102
BceAI ACGGC 4 cut(s) 414, 437, 498, 920
BcgI CGANNNNNNTGC 2 cut(s) 431, 465
BciVI GTATCC 2 cut(s) 595, 804
BcnI CCSGG 1 cut(s) 429
BcoDI GTCTC 6 cut(s) 645, 765, 861, 1021, 1054, 1229
BfaI CTAG 4 cut(s) 315, 533, 1131, 1245
BfmI CTRYAG 1 cut(s) 960
BfoI RGCGCY 1 cut(s) 379
BfuI GTATCC 2 cut(s) 595, 804
BisI GCNGC 3 cut(s) 486, 516, 1050
BlsI GCNGC 3 cut(s) 487, 517, 1051
Bme1390I CCNGG 1 cut(s) 429
Bme18I GGWCC 2 cut(s) 498, 1241
BmeT110I CYCGRG 2 cut(s) 257, 1085
BmgBI CACGTC 1 cut(s) 1064
BmgT120I GGNCC 6 cut(s) 498, 554, 555, 580, 616, 1241
BmiI GGNNCC 4 cut(s) 500, 556, 955, 1242
BmrFI CCNGG 1 cut(s) 429
BmsI GCATC 4 cut(s) 333, 772, 791, 1305
BpiI GAAGAC 1 cut(s) 543
BpuMI CCSGG 1 cut(s) 429
Bsa29I ATCGAT 1 cut(s) 1157
BsaAI YACGTR 4 cut(s) 244, 382, 478, 817
BsaBI GATNNNNATC 1 cut(s) 180
BsaJI CCNNGG 3 cut(s) 23, 71, 1319
BsaWI WCCGGW 2 cut(s) 413, 679
BsaXI ACNNNNNCTCC 8 cut(s) 666, 696, 1269, 1272, 1299, 1302, 1315, 1345
Bsc4I CCNNNNNNNGG 1 cut(s) 589
Bse1I ACTGG 1 cut(s) 156
Bse8I GATNNNNATC 1 cut(s) 180
BseCI ATCGAT 1 cut(s) 1157
BseDI CCNNGG 3 cut(s) 23, 71, 1319
BseGI GGATG 4 cut(s) 238, 476, 1129, 1225
BseJI GATNNNNATC 1 cut(s) 180
BseLI CCNNNNNNNGG 1 cut(s) 589
BseMII CTCAG 2 cut(s) 690, 1161
BseNI ACTGG 1 cut(s) 156
BseRI GAGGAG 2 cut(s) 1293, 1336
BseSI GKGCMC 4 cut(s) 110, 558, 1001, 1181
BseXI GCAGC 2 cut(s) 472, 1036
Bsh1236I CGCG 1 cut(s) 131
Bsh1285I CGRYCG 2 cut(s) 277, 684
BshFI GGCC 4 cut(s) 401, 556, 581, 617
BshVI ATCGAT 1 cut(s) 1157
BsiEI CGRYCG 2 cut(s) 277, 684
BsiHKAI GWGCWC 2 cut(s) 110, 1181
BsiHKCI CYCGRG 2 cut(s) 257, 1085
BsiSI CCGG 4 cut(s) 414, 428, 680, 942
BslI CCNNNNNNNGG 1 cut(s) 589
BsmAI GTCTC 6 cut(s) 645, 765, 861, 1021, 1054, 1229
BsmBI CGTCTC 2 cut(s) 1054, 1229
BsnI GGCC 4 cut(s) 401, 556, 581, 617
BsoBI CYCGRG 2 cut(s) 257, 1085
Bsp120I GGGCCC 1 cut(s) 554
Bsp1286I GDGCHC 5 cut(s) 110, 558, 694, 1001, 1181
Bsp143I GATC 4 cut(s) 175, 181, 592, 1424
BspACI CCGC 5 cut(s) 515, 1196, 1259, 1283, 1295
BspANI GGCC 4 cut(s) 401, 556, 581, 617
BspCNI CTCAG 2 cut(s) 691, 1162
BspDI ATCGAT 1 cut(s) 1157
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 4 cut(s) 500, 556, 955, 1242
BspMAI CTGCAG 1 cut(s) 964
BspPI GGATC 1 cut(s) 600
BsrBI CCGCTC 2 cut(s) 515, 1283
BsrI ACTGG 1 cut(s) 156
BssECI CCNNGG 3 cut(s) 23, 71, 1319
BssMI GATC 4 cut(s) 175, 181, 592, 1424
BssSI CACGAG 2 cut(s) 506, 713
BssT1I CCWWGG 1 cut(s) 71
Bst2BI CACGAG 2 cut(s) 506, 713
Bst4CI ACNGT 4 cut(s) 551, 607, 895, 1151
BstBAI YACGTR 4 cut(s) 244, 382, 478, 817
BstC8I GCNNGC 2 cut(s) 513, 1181
BstDEI CTNAG 4 cut(s) 699, 1031, 1170, 1365
BstF5I GGATG 4 cut(s) 238, 476, 1129, 1225
BstFNI CGCG 1 cut(s) 131
BstH2I RGCGCY 1 cut(s) 379
BstHHI GCGC 2 cut(s) 131, 378
BstKTI GATC 4 cut(s) 178, 184, 595, 1427
BstMAI GTCTC 6 cut(s) 645, 765, 861, 1021, 1054, 1229
BstMBI GATC 4 cut(s) 175, 181, 592, 1424
BstMCI CGRYCG 2 cut(s) 277, 684
BstMWI GCNNNNNNNGC 2 cut(s) 491, 756
BstNSI RCATGY 1 cut(s) 834
BstSCI CCNGG 1 cut(s) 427
BstSFI CTRYAG 1 cut(s) 960
BstSLI GKGCMC 4 cut(s) 110, 558, 1001, 1181
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 2 cut(s) 472, 1036
BstV2I GAAGAC 1 cut(s) 543
Bsu15I ATCGAT 1 cut(s) 1157
BsuI GTATCC 2 cut(s) 595, 804
BsuRI GGCC 4 cut(s) 401, 556, 581, 617
BsuTUI ATCGAT 1 cut(s) 1157
BtgZI GCGATG 2 cut(s) 648, 795
BtrI CACGTC 1 cut(s) 1064
BtsCI GGATG 4 cut(s) 238, 476, 1129, 1225
BtsI GCAGTG 1 cut(s) 957
BtsIMutI CAGTG 2 cut(s) 696, 957
Cac8I GCNNGC 2 cut(s) 513, 1181
CfoI GCGC 2 cut(s) 131, 378
Cfr13I GGNCC 6 cut(s) 498, 554, 555, 580, 616, 1241
ClaI ATCGAT 1 cut(s) 1157
Csp6I GTAC 4 cut(s) 139, 455, 724, 1265
CviAII CATG 6 cut(s) 157, 229, 495, 610, 644, 831
CviQI GTAC 4 cut(s) 139, 455, 724, 1265
DdeI CTNAG 4 cut(s) 699, 1031, 1170, 1365
DpnI GATC 4 cut(s) 177, 183, 594, 1426
DpnII GATC 4 cut(s) 175, 181, 592, 1424
DraIII CACNNNGTG 1 cut(s) 897
Eco130I CCWWGG 1 cut(s) 71
Eco24I GRGCYC 2 cut(s) 558, 694
Eco47I GGWCC 2 cut(s) 498, 1241
Eco72I CACGTG 4 cut(s) 244, 382, 478, 817
Eco88I CYCGRG 2 cut(s) 257, 1085
EcoO109I RGGNCCY 1 cut(s) 1241
EcoT14I CCWWGG 1 cut(s) 71
EcoT38I GRGCYC 2 cut(s) 558, 694
ErhI CCWWGG 1 cut(s) 71
Esp3I CGTCTC 2 cut(s) 1054, 1229
FaeI CATG 6 cut(s) 160, 232, 498, 613, 647, 834
FatI CATG 6 cut(s) 156, 228, 494, 609, 643, 830
FblI GTMKAC 2 cut(s) 84, 433
Fnu4HI GCNGC 3 cut(s) 486, 516, 1050
FokI GGATG 4 cut(s) 245, 483, 1136, 1232
FriOI GRGCYC 2 cut(s) 558, 694
Fsp4HI GCNGC 3 cut(s) 486, 516, 1050
FspBI CTAG 4 cut(s) 315, 533, 1131, 1245
GlaI GCGC 2 cut(s) 130, 377
GluI GCNGC 3 cut(s) 486, 516, 1050
HaeII RGCGCY 1 cut(s) 379
HaeIII GGCC 4 cut(s) 401, 556, 581, 617
HapII CCGG 4 cut(s) 414, 428, 680, 942
HhaI GCGC 2 cut(s) 131, 378
Hin1II CATG 6 cut(s) 160, 232, 498, 613, 647, 834
Hin6I GCGC 2 cut(s) 129, 376
HinP1I GCGC 2 cut(s) 129, 376
HincII GTYRAC 2 cut(s) 387, 1413
HindII GTYRAC 2 cut(s) 387, 1413
HinfI GANTC 6 cut(s) 32, 44, 60, 92, 417, 1090
HpaII CCGG 4 cut(s) 414, 428, 680, 942
HphI GGTGA 5 cut(s) 262, 535, 862, 1199, 1244
Hpy166II GTNNAC 6 cut(s) 85, 108, 387, 434, 1179, 1413
Hpy188I TCNGA 9 cut(s) 27, 37, 97, 214, 442, 463, 770, 1095, 1123
Hpy188III TCNNGA 9 cut(s) 55, 179, 259, 533, 599, 652, 713, 1059, 1087
Hpy8I GTNNAC 6 cut(s) 85, 108, 387, 434, 1179, 1413
Hpy99I CGWCG 6 cut(s) 56, 653, 665, 708, 941, 1339
HpyAV CCTTC 2 cut(s) 725, 1007
HpyCH4III ACNGT 4 cut(s) 551, 607, 895, 1151
HpyCH4IV ACGT 7 cut(s) 243, 381, 477, 816, 1001, 1063, 1415
HpyCH4V TGCA 6 cut(s) 108, 346, 370, 494, 962, 1179
HpyF10VI GCNNNNNNNGC 2 cut(s) 491, 756
HpyF3I CTNAG 4 cut(s) 699, 1031, 1170, 1365
HpySE526I ACGT 7 cut(s) 243, 381, 477, 816, 1001, 1063, 1415
Hsp92II CATG 6 cut(s) 160, 232, 498, 613, 647, 834
HspAI GCGC 2 cut(s) 129, 376
Kzo9I GATC 4 cut(s) 175, 181, 592, 1424
LmnI GCTCC 4 cut(s) 944, 959, 1280, 1351
Lsp1109I GCAGC 2 cut(s) 472, 1036
LweI GCATC 4 cut(s) 333, 772, 791, 1305
MaeI CTAG 4 cut(s) 315, 533, 1131, 1245
MaeII ACGT 7 cut(s) 243, 381, 477, 816, 1001, 1063, 1415
MaeIII GTNAC 8 cut(s) 200, 244, 473, 478, 817, 912, 1126, 1213
MalI GATC 4 cut(s) 177, 183, 594, 1426
MbiI CCGCTC 2 cut(s) 515, 1283
MboI GATC 4 cut(s) 175, 181, 592, 1424
MboII GAAGA 3 cut(s) 548, 810, 1000
MhlI GDGCHC 5 cut(s) 110, 558, 694, 1001, 1181
MluCI AATT 4 cut(s) 745, 917, 1271, 1397
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 3 cut(s) 486, 1101, 1288
MroXI GAANNNNTTC 1 cut(s) 749
MseI TTAA 4 cut(s) 117, 546, 858, 1299
MslI CAYNNNNRTG 1 cut(s) 233
MspA1I CMGCKG 1 cut(s) 1295
MspI CCGG 4 cut(s) 414, 428, 680, 942
MspR9I CCNGG 1 cut(s) 429
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 2 cut(s) 491, 756
NciI CCSGG 1 cut(s) 429
NdeII GATC 4 cut(s) 175, 181, 592, 1424
NlaIII CATG 6 cut(s) 160, 232, 498, 613, 647, 834
NlaIV GGNNCC 4 cut(s) 500, 556, 955, 1242
NmeAIII GCCGAG 1 cut(s) 1344
NmuCI GTSAC 5 cut(s) 244, 473, 478, 817, 1126
NspI RCATGY 1 cut(s) 834
PaeR7I CTCGAG 1 cut(s) 1085
PcsI WCGNNNNNNNCGW 2 cut(s) 657, 822
PdmI GAANNNNTTC 1 cut(s) 749
PfeI GAWTC 5 cut(s) 32, 44, 60, 417, 1090
PflFI GACNNNGTC 1 cut(s) 429
PkrI GCNGC 3 cut(s) 487, 517, 1051
PleI GAGTC 1 cut(s) 86
PmaCI CACGTG 4 cut(s) 244, 382, 478, 817
PmlI CACGTG 4 cut(s) 244, 382, 478, 817
PpsI GAGTC 1 cut(s) 86
Ppu21I YACGTR 4 cut(s) 244, 382, 478, 817
PpuMI RGGWCCY 1 cut(s) 1241
Psp5II RGGWCCY 1 cut(s) 1241
PspCI CACGTG 4 cut(s) 244, 382, 478, 817
PspN4I GGNNCC 4 cut(s) 500, 556, 955, 1242
PspOMI GGGCCC 1 cut(s) 554
PspPI GGNCC 6 cut(s) 498, 554, 555, 580, 616, 1241
PspPPI RGGWCCY 1 cut(s) 1241
PstI CTGCAG 1 cut(s) 964
PsyI GACNNNGTC 1 cut(s) 429
RsaI GTAC 4 cut(s) 140, 456, 725, 1266
RsaNI GTAC 4 cut(s) 139, 455, 724, 1265
RseI CAYNNNNRTG 1 cut(s) 233
SaqAI TTAA 4 cut(s) 117, 546, 858, 1299
SatI GCNGC 3 cut(s) 486, 516, 1050
Sau3AI GATC 4 cut(s) 175, 181, 592, 1424
Sau96I GGNCC 6 cut(s) 498, 554, 555, 580, 616, 1241
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 1 cut(s) 429
SduI GDGCHC 5 cut(s) 110, 558, 694, 1001, 1181
SfaNI GCATC 4 cut(s) 333, 772, 791, 1305
SfcI CTRYAG 1 cut(s) 960
Sfr274I CTCGAG 1 cut(s) 1085
SinI GGWCC 2 cut(s) 498, 1241
SlaI CTCGAG 1 cut(s) 1085
SmiMI CAYNNNNRTG 1 cut(s) 233
SmlI CTYRAG 1 cut(s) 1085
SmoI CTYRAG 1 cut(s) 1085
Sse9I AATT 4 cut(s) 745, 917, 1271, 1397
SsiI CCGC 5 cut(s) 515, 1196, 1259, 1283, 1295
SspI AATATT 1 cut(s) 974
SspMI CTAG 4 cut(s) 315, 533, 1131, 1245
StyD4I CCNGG 1 cut(s) 427
StyI CCWWGG 1 cut(s) 71
TaaI ACNGT 4 cut(s) 551, 607, 895, 1151
TaiI ACGT 7 cut(s) 246, 384, 480, 819, 1004, 1066, 1418
TaqII GACCGA 1 cut(s) 291
TasI AATT 4 cut(s) 745, 917, 1271, 1397
TauI GCSGC 1 cut(s) 518
TfiI GAWTC 5 cut(s) 32, 44, 60, 417, 1090
Tru1I TTAA 4 cut(s) 117, 546, 858, 1299
Tru9I TTAA 4 cut(s) 117, 546, 858, 1299
TscAI CASTG 2 cut(s) 703, 964
TseFI GTSAC 5 cut(s) 244, 473, 478, 817, 1126
TseI GCWGC 2 cut(s) 485, 1049
Tsp45I GTSAC 5 cut(s) 244, 473, 478, 817, 1126
TspDTI ATGAA 2 cut(s) 217, 967
TspGWI ACGGA 3 cut(s) 741, 1025, 1232
TspRI CASTG 2 cut(s) 703, 964
Tth111I GACNNNGTC 1 cut(s) 429
VneI GTGCAC 2 cut(s) 106, 1177
VpaK11BI GGWCC 2 cut(s) 498, 1241
XbaI TCTAGA 1 cut(s) 532
XceI RCATGY 1 cut(s) 834
XcmI CCANNNNNNNNNTGG 1 cut(s) 626
XhoI CTCGAG 1 cut(s) 1085
XmiI GTMKAC 2 cut(s) 84, 433
XmnI GAANNNNTTC 1 cut(s) 749
XspI CTAG 4 cut(s) 315, 533, 1131, 1245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.