MD02G1188900.v1.1

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007417
Physical Location & Seq
Reverse (-)
17257096 .. 17261825
4730 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1188900.v1.1.491

Sequence Viewer

Length: 1431 bp
ATGGAAATAATTTACCAGCCCTATATTGATCCGGAGTTCGAATACCTCATTGAAAGAATCTATCCTCCATGGGTTTGTATCGATAATGACTCATACAAAGATTGCACGCTTGTGAAGGTTGACAGTGCAAACAAGCACGGGATTTTACTGGAAATGGTCCAAGTTTTGACTGATCTTGATCTTCTTATTTCTAAGTCGTACATATCATCCGATGGTGGATGGTTTATGGACGTGTTCCACGTGACGGACCAACTCGGAAACAAAATCACTGACGAAAGCCTCATACTCTATATTCAGCAGGCACTGTGTGCAAGGAGAGGTGAGATTTCAAAACAGGCGAGAGTATGCCTACGAAGAGAAGTGACACCGGAACTGAAGGACTTGGCCTTAGAGATGACCAGCAAGGACCGACCGGGACTCATGTCCGAAATATCAGCGGTCCTTGTGGAGTTGGGCTGCCACATTACTGCTGCCATGGCTTGGACCCACAGTGCCCGGGCAGCCGGCATTTTCTACATCGAAGACGGGCTGAAAGGAGGGCCAATTACGGACCCAGTAAGACTTGCCCATGTAGAGGAGCAGCTGGAGAATGTGATTGAGGCCCATCATGAGGAGGGTGAGAAGAGGAGCGTAAAGCTGACCACCCCGGCGGGCGGCCACACCCACACGGAGCGGCGGCTACACCAGTTGATGTATGCTGATGACGACTACGAAAGCTGTCGTGGTTGCGACGGAGGTGGTAGTAGTGCACACAAGAGGGGTTGCGATCGGACACATGTGAACATCGAGAGTTGTAAGGAGAAAGGGTATTGGGTTGTGAACGTGAGCTGCCGAGACCGTCCTAAGCTACTGTTTGACACGGTGTGCGCCCTAACCGACTTGCAGTACGTGGTGTTCCATGCGGTGGTTTCCTCCAGGGGCACCATGGCTGATCAGGAGTATTTTGTACGGCACAAGGACGGATACACTTTGAACACAGAGAGTGAAAGACACAAGCTTACCCTGTGCTTGATTGCGGCAATAGAGAGGAGGATCTCACAGGGACTGAGGCTAGACCTCTGTGCCAAAAACCGAATGGGGCTGTTTTCTGACATCACTAGGGTTCTGAGAGAGTACGGGTTATCAATATCAAGATTCTCACTTGGAATACACGGTGAGAAAGCTGTAGGATCAATCTATGTCACAGATGCCTCAGGACACGATATAAGTCCGAGCACGGTGGAGATGGTGACAAAGGAGATTGGAGAATCCGTCATCGCAGTTCACAAATCACAGAAGTGGGCTCTTCAGGGTTCCTCGTCGAGGACGAGCCAGAAGGCAGCCAAAAGAAGAGAAGAAGAAGAAGAAGAAAGACCAAGATTTTCACTTGGAGGCTTGGTTTGGTCTCATCTGGAGCGTCTTTCCAGCAGTTTTGGACCCATAAGATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0016597 GO:0031406 GO:0036094 GO:0043167 GO:0043168 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT ACT_6

Protein Analysis

477

Amino Acids

53.54

Weight (kDa)

5.97

Isoelectric Point (pI)

56.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 354 - 408 2.3e-06 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 920
AccB7I CCANNNNNTGG 2 cut(s) 480, 904
AccBSI CCGCTC 1 cut(s) 673
AccIII TCCGGA 1 cut(s) 31
AciI CCGC 7 cut(s) 437, 650, 654, 673, 676, 902, 1016
AclWI GGATC 3 cut(s) 23, 1040, 1177
AcoI YGGCCR 1 cut(s) 655
AcuI CTGAAG 2 cut(s) 395, 1271
AcvI CACGTG 1 cut(s) 241
AdeI CACNNNGTG 2 cut(s) 308, 864
AfaI GTAC 4 cut(s) 200, 887, 948, 1115
AfiI CCNNNNNNNGG 7 cut(s) 244, 480, 574, 610, 653, 904, 1302
AflIII ACRYGT 2 cut(s) 231, 775
AgsI TTSAA 3 cut(s) 53, 330, 973
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 1 cut(s) 914
AleI CACNNNNGTG 2 cut(s) 110, 1276
AluBI AGCT 7 cut(s) 583, 637, 717, 828, 847, 997, 1163
AluI AGCT 7 cut(s) 583, 637, 717, 828, 847, 997, 1163
Alw21I GWGCWC 2 cut(s) 751, 1217
Alw26I GTCTC 2 cut(s) 828, 1389
Alw44I GTGCAC 1 cut(s) 747
AlwI GGATC 3 cut(s) 23, 1040, 1177
AlwNI CAGNNNCTG 2 cut(s) 304, 1045
Ama87I CYCGRG 1 cut(s) 495
Aor13HI TCCGGA 1 cut(s) 31
AoxI GGCC 4 cut(s) 384, 539, 600, 655
ApaLI GTGCAC 1 cut(s) 747
ApeKI GCWGC 6 cut(s) 456, 470, 500, 580, 828, 1319
AspLEI GCGC 1 cut(s) 869
AspS9I GGNCC 9 cut(s) 157, 247, 406, 439, 483, 539, 550, 601, 1415
AsuC2I CCSGG 4 cut(s) 414, 496, 497, 647
AsuHPI GGTGA 4 cut(s) 332, 629, 1166, 1240
AsuII TTCGAA 1 cut(s) 39
AvaI CYCGRG 1 cut(s) 495
AvaII GGWCC 7 cut(s) 157, 247, 406, 439, 483, 550, 1415
AxyI CCTNAGG 1 cut(s) 1192
BaeGI GKGCMC 3 cut(s) 496, 751, 923
BanI GGYRCC 1 cut(s) 920
BanII GRGCYC 1 cut(s) 1285
BbrPI CACGTG 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 2 cut(s) 751, 1217
BbvI GCAGC 6 cut(s) 443, 457, 512, 592, 815, 1331
BccI CCATC 4 cut(s) 206, 213, 612, 1219
BceAI ACGGC 1 cut(s) 965
BciT130I CCWGG 1 cut(s) 916
BciVI GTATCC 1 cut(s) 956
BclI TGATCA 1 cut(s) 931
BcnI CCSGG 4 cut(s) 414, 496, 497, 647
BcoDI GTCTC 2 cut(s) 828, 1389
BfaI CTAG 2 cut(s) 1052, 1098
BfmI CTRYAG 1 cut(s) 1164
BfuI GTATCC 1 cut(s) 956
Bme1390I CCNGG 5 cut(s) 414, 496, 497, 647, 916
Bme18I GGWCC 7 cut(s) 157, 247, 406, 439, 483, 550, 1415
BmeT110I CYCGRG 1 cut(s) 495
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 9 cut(s) 157, 247, 406, 439, 483, 539, 550, 601, 1415
BmiI GGNNCC 5 cut(s) 485, 552, 922, 1294, 1417
BmrFI CCNGG 5 cut(s) 414, 496, 497, 647, 916
BmrI ACTGGG 1 cut(s) 548
BmsI GCATC 1 cut(s) 1177
BmuI ACTGGG 1 cut(s) 548
BoxI GACNNNNGTC 1 cut(s) 421
BpiI GAAGAC 1 cut(s) 528
BpmI CTGGAG 3 cut(s) 605, 898, 1412
Bpu10I CCTNAGC 1 cut(s) 843
Bpu14I TTCGAA 1 cut(s) 39
BpuMI CCSGG 4 cut(s) 414, 496, 497, 647
Bsa29I ATCGAT 1 cut(s) 81
BsaAI YACGTR 2 cut(s) 241, 889
BsaBI GATNNNNATC 1 cut(s) 177
BsaI GGTCTC 2 cut(s) 828, 1389
BsaJI CCNNGG 6 cut(s) 68, 474, 495, 645, 915, 924
BsaWI WCCGGW 2 cut(s) 31, 367
BsaXI ACNNNNNCTCC 6 cut(s) 791, 821, 1236, 1266, 1362, 1392
Bsc4I CCNNNNNNNGG 7 cut(s) 244, 480, 574, 610, 653, 904, 1302
Bse118I RCCGGY 1 cut(s) 503
Bse1I ACTGG 3 cut(s) 153, 554, 685
Bse21I CCTNAGG 1 cut(s) 1192
Bse8I GATNNNNATC 1 cut(s) 177
BseAI TCCGGA 1 cut(s) 31
BseBI CCWGG 1 cut(s) 916
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 6 cut(s) 68, 474, 495, 645, 915, 924
BseGI GGATG 2 cut(s) 206, 224
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 7 cut(s) 244, 480, 574, 610, 653, 904, 1302
BseMII CTCAG 3 cut(s) 1037, 1097, 1206
BseNI ACTGG 3 cut(s) 153, 554, 685
BseRI GAGGAG 4 cut(s) 590, 626, 640, 1042
BseSI GKGCMC 3 cut(s) 496, 751, 923
BseXI GCAGC 6 cut(s) 443, 457, 512, 592, 815, 1331
Bsh1285I CGRYCG 2 cut(s) 413, 769
BshFI GGCC 4 cut(s) 386, 541, 602, 657
BshNI GGYRCC 1 cut(s) 920
BshVI ATCGAT 1 cut(s) 81
BsiEI CGRYCG 2 cut(s) 413, 769
BsiHKAI GWGCWC 2 cut(s) 751, 1217
BsiHKCI CYCGRG 1 cut(s) 495
BsiSI CCGG 6 cut(s) 32, 368, 413, 496, 504, 647
BslFI GGGAC 2 cut(s) 429, 1056
BslI CCNNNNNNNGG 7 cut(s) 244, 480, 574, 610, 653, 904, 1302
BsmAI GTCTC 2 cut(s) 828, 1389
BsmFI GGGAC 2 cut(s) 429, 1056
BsnI GGCC 4 cut(s) 386, 541, 602, 657
Bso31I GGTCTC 2 cut(s) 828, 1389
BsoBI CYCGRG 1 cut(s) 495
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 5 cut(s) 496, 751, 923, 1217, 1285
Bsp13I TCCGGA 1 cut(s) 31
Bsp143I GATC 8 cut(s) 28, 172, 178, 766, 931, 1032, 1169, 1424
Bsp19I CCATGG 3 cut(s) 68, 474, 924
BspACI CCGC 7 cut(s) 437, 650, 654, 673, 676, 902, 1016
BspANI GGCC 4 cut(s) 386, 541, 602, 657
BspCNI CTCAG 3 cut(s) 1038, 1098, 1205
BspDI ATCGAT 1 cut(s) 81
BspEI TCCGGA 1 cut(s) 31
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 5 cut(s) 485, 552, 922, 1294, 1417
BspPI GGATC 3 cut(s) 23, 1040, 1177
BspQI GCTCTTC 1 cut(s) 1290
BspT104I TTCGAA 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 920
BspTNI GGTCTC 2 cut(s) 828, 1389
BsrBI CCGCTC 1 cut(s) 673
BsrFI RCCGGY 1 cut(s) 503
BsrI ACTGG 3 cut(s) 153, 554, 685
BssAI RCCGGY 1 cut(s) 503
BssECI CCNNGG 6 cut(s) 68, 474, 495, 645, 915, 924
BssMI GATC 8 cut(s) 28, 172, 178, 766, 931, 1032, 1169, 1424
BssT1I CCWWGG 3 cut(s) 68, 474, 924
Bst2UI CCWGG 1 cut(s) 916
Bst4CI ACNGT 8 cut(s) 125, 306, 491, 839, 852, 862, 1154, 1219
Bst6I CTCTTC 4 cut(s) 349, 617, 1290, 1324
BstAPI GCANNNNNTGC 1 cut(s) 308
BstBAI YACGTR 2 cut(s) 241, 889
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 4 cut(s) 107, 300, 505, 652
BstDEI CTNAG 6 cut(s) 192, 388, 843, 1046, 1106, 1192
BstDSI CCRYGG 3 cut(s) 68, 474, 924
BstENI CCTNNNNNAGG 1 cut(s) 1300
BstF5I GGATG 2 cut(s) 206, 224
BstHHI GCGC 1 cut(s) 869
BstKTI GATC 8 cut(s) 31, 175, 181, 769, 934, 1035, 1172, 1427
BstMAI GTCTC 2 cut(s) 828, 1389
BstMBI GATC 8 cut(s) 28, 172, 178, 766, 931, 1032, 1169, 1424
BstMCI CGRYCG 2 cut(s) 413, 769
BstMWI GCNNNNNNNGC 3 cut(s) 308, 476, 500
BstNI CCWGG 1 cut(s) 916
BstNSI RCATGY 1 cut(s) 779
BstPAI GACNNNNGTC 1 cut(s) 421
BstSCI CCNGG 5 cut(s) 412, 494, 495, 645, 914
BstSFI CTRYAG 1 cut(s) 1164
BstSLI GKGCMC 3 cut(s) 496, 751, 923
BstV1I GCAGC 6 cut(s) 443, 457, 512, 592, 815, 1331
BstV2I GAAGAC 1 cut(s) 528
BstX2I RGATCY 1 cut(s) 1032
BstYI RGATCY 1 cut(s) 1032
Bsu15I ATCGAT 1 cut(s) 81
Bsu36I CCTNAGG 1 cut(s) 1192
BsuI GTATCC 1 cut(s) 956
BsuRI GGCC 4 cut(s) 386, 541, 602, 657
BsuTUI ATCGAT 1 cut(s) 81
BtgI CCRYGG 3 cut(s) 68, 474, 924
BtgZI GCGATG 1 cut(s) 1240
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 2 cut(s) 206, 224
BtsIMutI CAGTG 4 cut(s) 130, 267, 302, 496
Cac8I GCNNGC 4 cut(s) 107, 300, 505, 652
CaiI CAGNNNCTG 2 cut(s) 304, 1045
CciI TCATGA 1 cut(s) 607
CfoI GCGC 1 cut(s) 869
Cfr10I RCCGGY 1 cut(s) 503
Cfr13I GGNCC 9 cut(s) 157, 247, 406, 439, 483, 539, 550, 601, 1415
Cfr9I CCCGGG 1 cut(s) 495
ClaI ATCGAT 1 cut(s) 81
CseI GACGC 1 cut(s) 1385
Csp6I GTAC 4 cut(s) 199, 886, 947, 1114
CviAII CATG 8 cut(s) 69, 421, 475, 569, 608, 776, 899, 925
CviQI GTAC 4 cut(s) 199, 886, 947, 1114
DdeI CTNAG 6 cut(s) 192, 388, 843, 1046, 1106, 1192
DpnI GATC 8 cut(s) 30, 174, 180, 768, 933, 1034, 1171, 1426
DpnII GATC 8 cut(s) 28, 172, 178, 766, 931, 1032, 1169, 1424
DraIII CACNNNGTG 2 cut(s) 308, 864
EaeI YGGCCR 1 cut(s) 655
Eam1104I CTCTTC 4 cut(s) 349, 617, 1290, 1324
EarI CTCTTC 4 cut(s) 349, 617, 1290, 1324
Eco130I CCWWGG 3 cut(s) 68, 474, 924
Eco24I GRGCYC 1 cut(s) 1285
Eco31I GGTCTC 2 cut(s) 828, 1389
Eco47I GGWCC 7 cut(s) 157, 247, 406, 439, 483, 550, 1415
Eco57I CTGAAG 2 cut(s) 395, 1271
Eco72I CACGTG 1 cut(s) 241
Eco81I CCTNAGG 1 cut(s) 1192
Eco88I CYCGRG 1 cut(s) 495
EcoNI CCTNNNNNAGG 1 cut(s) 1300
EcoRII CCWGG 1 cut(s) 914
EcoT14I CCWWGG 3 cut(s) 68, 474, 924
EcoT38I GRGCYC 1 cut(s) 1285
ErhI CCWWGG 3 cut(s) 68, 474, 924
FaeI CATG 8 cut(s) 72, 424, 478, 572, 611, 779, 902, 928
FaqI GGGAC 2 cut(s) 429, 1056
FatI CATG 8 cut(s) 68, 420, 474, 568, 607, 775, 898, 924
FauI CCCGC 1 cut(s) 643
FbaI TGATCA 1 cut(s) 931
FokI GGATG 2 cut(s) 193, 231
FriOI GRGCYC 1 cut(s) 1285
FspBI CTAG 2 cut(s) 1052, 1098
GlaI GCGC 1 cut(s) 868
GsuI CTGGAG 3 cut(s) 605, 898, 1412
HaeIII GGCC 4 cut(s) 386, 541, 602, 657
HapII CCGG 6 cut(s) 32, 368, 413, 496, 504, 647
HgaI GACGC 1 cut(s) 1385
HhaI GCGC 1 cut(s) 869
Hin1II CATG 8 cut(s) 72, 424, 478, 572, 611, 779, 902, 928
Hin6I GCGC 1 cut(s) 867
HinP1I GCGC 1 cut(s) 867
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HindIII AAGCTT 1 cut(s) 995
HinfI GANTC 5 cut(s) 57, 89, 417, 1134, 1247
HpaII CCGG 6 cut(s) 32, 368, 413, 496, 504, 647
HphI GGTGA 4 cut(s) 332, 629, 1166, 1240
Hpy166II GTNNAC 5 cut(s) 121, 749, 781, 820, 1264
Hpy188I TCNGA 7 cut(s) 211, 257, 427, 771, 1090, 1107, 1212
Hpy188III TCNNGA 8 cut(s) 32, 176, 608, 787, 935, 1131, 1194, 1391
Hpy8I GTNNAC 5 cut(s) 121, 749, 781, 820, 1264
Hpy99I CGWCG 2 cut(s) 734, 1303
HpyAV CCTTC 3 cut(s) 109, 370, 1309
HpyCH4III ACNGT 8 cut(s) 125, 306, 491, 839, 852, 862, 1154, 1219
HpyCH4IV ACGT 4 cut(s) 231, 240, 822, 888
HpyCH4V TGCA 5 cut(s) 105, 128, 311, 749, 883
HpyF10VI GCNNNNNNNGC 3 cut(s) 308, 476, 500
HpyF3I CTNAG 6 cut(s) 192, 388, 843, 1046, 1106, 1192
HpySE526I ACGT 4 cut(s) 231, 240, 822, 888
Hsp92II CATG 8 cut(s) 72, 424, 478, 572, 611, 779, 902, 928
HspAI GCGC 1 cut(s) 867
Kpn2I TCCGGA 1 cut(s) 31
KroI GCCGGC 1 cut(s) 503
KroNI GCCGGC 1 cut(s) 505
Ksp22I TGATCA 1 cut(s) 931
Kzo9I GATC 8 cut(s) 28, 172, 178, 766, 931, 1032, 1169, 1424
LguI GCTCTTC 1 cut(s) 1290
LmnI GCTCC 4 cut(s) 577, 627, 670, 1393
Lsp1109I GCAGC 6 cut(s) 443, 457, 512, 592, 815, 1331
LweI GCATC 1 cut(s) 1177
MaeI CTAG 2 cut(s) 1052, 1098
MaeII ACGT 4 cut(s) 231, 240, 822, 888
MaeIII GTNAC 4 cut(s) 241, 361, 1180, 1228
MalI GATC 8 cut(s) 30, 174, 180, 768, 933, 1034, 1171, 1426
MbiI CCGCTC 1 cut(s) 673
MboI GATC 8 cut(s) 28, 172, 178, 766, 931, 1032, 1169, 1424
MflI RGATCY 1 cut(s) 1032
MhlI GDGCHC 5 cut(s) 496, 751, 923, 1217, 1285
MluCI AATT 2 cut(s) 9, 543
MlyI GAGTC 2 cut(s) 83, 411
MroI TCCGGA 1 cut(s) 31
MroNI GCCGGC 1 cut(s) 503
MslI CAYNNNNRTG 2 cut(s) 110, 1276
MspA1I CMGCKG 2 cut(s) 437, 583
MspI CCGG 6 cut(s) 32, 368, 413, 496, 504, 647
MspR9I CCNGG 5 cut(s) 414, 496, 497, 647, 916
MvaI CCWGG 1 cut(s) 916
MwoI GCNNNNNNNGC 3 cut(s) 308, 476, 500
NaeI GCCGGC 1 cut(s) 505
NciI CCSGG 4 cut(s) 414, 496, 497, 647
NcoI CCATGG 3 cut(s) 68, 474, 924
NdeII GATC 8 cut(s) 28, 172, 178, 766, 931, 1032, 1169, 1424
NgoMIV GCCGGC 1 cut(s) 503
NlaIII CATG 8 cut(s) 72, 424, 478, 572, 611, 779, 902, 928
NlaIV GGNNCC 5 cut(s) 485, 552, 922, 1294, 1417
NmeAIII GCCGAG 1 cut(s) 857
NmuCI GTSAC 4 cut(s) 241, 361, 1180, 1228
NspI RCATGY 1 cut(s) 779
NspV TTCGAA 1 cut(s) 39
OliI CACNNNNGTG 2 cut(s) 110, 1276
PagI TCATGA 1 cut(s) 607
PciI ACATGT 1 cut(s) 775
PciSI GCTCTTC 1 cut(s) 1290
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 1304
PdiI GCCGGC 1 cut(s) 505
PfeI GAWTC 3 cut(s) 57, 1134, 1247
PflMI CCANNNNNTGG 2 cut(s) 480, 904
Ple19I CGATCG 1 cut(s) 769
PleI GAGTC 2 cut(s) 83, 411
PmaCI CACGTG 1 cut(s) 241
PmlI CACGTG 1 cut(s) 241
PpsI GAGTC 2 cut(s) 83, 411
Ppu21I YACGTR 2 cut(s) 241, 889
PscI ACATGT 1 cut(s) 775
PshAI GACNNNNGTC 1 cut(s) 421
Psp6I CCWGG 1 cut(s) 914
PspCI CACGTG 1 cut(s) 241
PspGI CCWGG 1 cut(s) 914
PspN4I GGNNCC 5 cut(s) 485, 552, 922, 1294, 1417
PspPI GGNCC 9 cut(s) 157, 247, 406, 439, 483, 539, 550, 601, 1415
PsrI GAACNNNNNNTAC 2 cut(s) 878, 910
PstNI CAGNNNCTG 2 cut(s) 304, 1045
PsuI RGATCY 1 cut(s) 1032
PvuI CGATCG 1 cut(s) 769
PvuII CAGCTG 1 cut(s) 583
RsaI GTAC 4 cut(s) 200, 887, 948, 1115
RsaNI GTAC 4 cut(s) 199, 886, 947, 1114
RseI CAYNNNNRTG 2 cut(s) 110, 1276
SapI GCTCTTC 1 cut(s) 1290
Sau3AI GATC 8 cut(s) 28, 172, 178, 766, 931, 1032, 1169, 1424
Sau96I GGNCC 9 cut(s) 157, 247, 406, 439, 483, 539, 550, 601, 1415
SchI GAGTC 2 cut(s) 83, 411
ScrFI CCNGG 5 cut(s) 414, 496, 497, 647, 916
SduI GDGCHC 5 cut(s) 496, 751, 923, 1217, 1285
SfaNI GCATC 1 cut(s) 1177
SfcI CTRYAG 1 cut(s) 1164
SfuI TTCGAA 1 cut(s) 39
SinI GGWCC 7 cut(s) 157, 247, 406, 439, 483, 550, 1415
SmaI CCCGGG 1 cut(s) 497
SmiMI CAYNNNNRTG 2 cut(s) 110, 1276
SrfI GCCCGGGC 1 cut(s) 497
Sse9I AATT 2 cut(s) 9, 543
SsiI CCGC 7 cut(s) 437, 650, 654, 673, 676, 902, 1016
SspMI CTAG 2 cut(s) 1052, 1098
StyD4I CCNGG 5 cut(s) 412, 494, 495, 645, 914
StyI CCWWGG 3 cut(s) 68, 474, 924
TaaI ACNGT 8 cut(s) 125, 306, 491, 839, 852, 862, 1154, 1219
TaiI ACGT 4 cut(s) 234, 243, 825, 891
TaqI TCGA 5 cut(s) 39, 81, 519, 786, 1301
TaqII GACCGA 1 cut(s) 423
TasI AATT 2 cut(s) 9, 543
TauI GCSGC 4 cut(s) 657, 676, 679, 1019
TfiI GAWTC 3 cut(s) 57, 1134, 1247
TscAI CASTG 4 cut(s) 130, 274, 309, 496
TseFI GTSAC 4 cut(s) 241, 361, 1180, 1228
TseI GCWGC 6 cut(s) 456, 470, 500, 580, 828, 1319
Tsp45I GTSAC 4 cut(s) 241, 361, 1180, 1228
TspGWI ACGGA 6 cut(s) 260, 563, 683, 747, 975, 1240
TspMI CCCGGG 1 cut(s) 495
TspRI CASTG 4 cut(s) 130, 274, 309, 496
Van91I CCANNNNNTGG 2 cut(s) 480, 904
VneI GTGCAC 1 cut(s) 747
VpaK11BI GGWCC 7 cut(s) 157, 247, 406, 439, 483, 550, 1415
XagI CCTNNNNNAGG 1 cut(s) 1300
XceI RCATGY 1 cut(s) 779
XcmI CCANNNNNNNNNTGG 2 cut(s) 922, 1072
XmaI CCCGGG 1 cut(s) 495
XspI CTAG 2 cut(s) 1052, 1098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.