Rorug02G0154300

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007417
Physical Location & Seq
Forward (+)
13698940 .. 13701354
2415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0154300.1

Sequence Viewer

Length: 1464 bp
ATGGGGGATTTATGCGAGGATTTGTGGGTTGAGATTTTGGGTAGACTATCATCGGAGAAGGACTTGGTGCCATGCATGAGCGTGTCCAGGTTATGGCACCGAATAACATCTGGTCTTTGGGTGCAGAGATTTTGGGCGCAGTCCCCTCCTTTGGGTCTTTTCTTCCGCACCGCCACCACCAGCACCACTACCGTAGACAAGGTATTGCATCGTATGAATTATATCTCTTTATATAATTATCATGCCATGGAAGATAACCTGCAAAGCACTCTTATTTGCGGAGGACAATTTTATAACAAAGAATCGTATAGGTCCAAAAAGTCTCATTGGCAGTATTTTGATCGACAATGGTATAAGCCGGGTGCCACTGCTGAATCCACTGATCGTGATGAGTACCTAAATGGTTGCAATGGTTTAGTTTTGTTAATGAAATCAGCAACTTATCAGTTTTATGTTTGCAATCCCATCACAAGACAACAAGTTGCCATACCTAAAGCCTGTGTCCACCAAAACCATGATGATCAGCATTTTTGTGCTGCTTTGGCTTTTGACCCTTCCGAATCTGCTCATCACTTCAGAGTTGTTAGAATTGATTATTCTGCATCCTACTCAACTGTGCTGGATATATTCTCGTCTGAATATGGACTGTGGGTTCGACATTGTCTGCAAATTGACCCAGGTTTTACTGAGGGATTCAAAAGCTCCAAGTTGTGCAGGCATTTTGTTTACTTGCGGGGCACGCTATACAGCATAGCCCTATCCTGGAACCTTTTATGCATTGACCTCAATACTGTTGCAGCTTCTGCCCTTGAACTTCCTGTTCCAGAGAATGAAAAGACCGGAACCATGGGATGTCTTGGGGTGTCGATGAATAGTCTCTGCTATATGAAGCGAATGCTCGATAATTCGAAGGACTTTCACAATCTGGCAGTTTGGTATAATGATGATGAATCTGGGGAATGGATTCTCAGATATAGTGTTTCTTGTCGCATATTGGGATACGGATTCCAGTCTCTAGGTTATGACTACGATGATTCCATGGAACCCTGTGCAATAAGTCCAACTTCAGATCTCTTGTTCTTTGGCAATCTCAACTTGATTTGTTGTATCCACCTTAAAAGCGAAAAGATGAAATTCGTTTGCGAAACAATCTCTAAAATAGATCCACCTGCTTCTTTCTTGACATTACGTGCATGCTTCGTACCTTTCTACACTCAGTTGAACAAGAGGAATGACGTCTCCACTCCATTAGCAACCGCTTGTGAGCGCACAAAAATTTTAAGTGTGGAAGCACCATACGGGAAACAAGAAGATGGTGAATCAGTTGAAGTAATAAATGTTAAGTTATATGATAGTTTGCAAAAAAAGCCATCCCCGTCATTGGTTTATCCCAGTGAAATCATGGGAATGCATTTAGAGCTTGAACATATCCCCCAATTTCTACTTGATACAATGGCAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0016597 GO:0031406 GO:0036094 GO:0043167 GO:0043168 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT ACT_6

Protein Analysis

487

Amino Acids

55.65

Weight (kDa)

5.85

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 6 - 42 9.5e-06 F-box-like
Beta-prop_KIB1-4 PF03478 128 - 279 8.2e-06 KIB1-4 beta-propeller
b-prop_At3g26010-like PF24750 129 - 334 3.4e-14 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 135 - 312 7.6e-09 F-box associated beta propeller domain
Beta-prop_AT5G49610-like PF23635 136 - 384 1.4e-07 F-box protein AT5G49610-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 294
AarI CACCTGC 1 cut(s) 1177
AatII GACGTC 1 cut(s) 1239
Acc36I ACCTGC 2 cut(s) 267, 1177
AccB1I GGYRCC 3 cut(s) 67, 96, 362
AccB7I CCANNNNNTGG 1 cut(s) 93
AccI GTMKAC 2 cut(s) 43, 195
AciI CCGC 5 cut(s) 166, 171, 279, 733, 1257
AclWI GGATC 1 cut(s) 1157
AcsI RAATTY 2 cut(s) 1133, 1275
AcuI CTGAAG 2 cut(s) 559, 1050
AcyI GRCGYC 1 cut(s) 1236
AfaI GTAC 2 cut(s) 395, 1203
AfiI CCNNNNNNNGG 5 cut(s) 93, 151, 152, 762, 1381
AgsI TTSAA 5 cut(s) 697, 812, 1222, 1328, 1424
AjnI CCWGG 3 cut(s) 86, 676, 761
AloI GAACNNNNNNTCC 2 cut(s) 636, 668
AluBI AGCT 3 cut(s) 702, 800, 1420
AluI AGCT 3 cut(s) 702, 800, 1420
Alw26I GTCTC 4 cut(s) 327, 881, 1017, 1243
AlwI GGATC 1 cut(s) 1157
AlwNI CAGNNNCTG 1 cut(s) 803
ApeKI GCWGC 2 cut(s) 536, 797
ApoI RAATTY 2 cut(s) 1133, 1275
ArsI GACNNNNNNTTYG 2 cut(s) 636, 668
Asp700I GAANNNNTTC 1 cut(s) 963
AspLEI GCGC 2 cut(s) 139, 1269
AspS9I GGNCC 1 cut(s) 312
AsuC2I CCSGG 1 cut(s) 360
AsuHPI GGTGA 1 cut(s) 1328
AsuII TTCGAA 1 cut(s) 908
AvaII GGWCC 1 cut(s) 312
BaeGI GKGCMC 1 cut(s) 740
BanI GGYRCC 3 cut(s) 67, 96, 362
BbvI GCAGC 2 cut(s) 523, 809
BccI CCATC 3 cut(s) 473, 1307, 1378
BciT130I CCWGG 3 cut(s) 88, 678, 763
BciVI GTATCC 2 cut(s) 992, 1118
BclI TGATCA 1 cut(s) 520
BcnI CCSGG 1 cut(s) 360
BcoDI GTCTC 4 cut(s) 327, 881, 1017, 1243
BfaI CTAG 1 cut(s) 1016
BfuAI ACCTGC 2 cut(s) 267, 1177
BfuI GTATCC 2 cut(s) 992, 1118
BglII AGATCT 1 cut(s) 1069
BisI GCNGC 2 cut(s) 537, 798
BlsI GCNGC 2 cut(s) 538, 799
Bme1390I CCNGG 4 cut(s) 88, 360, 678, 763
Bme18I GGWCC 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 312
BmiI GGNNCC 6 cut(s) 69, 98, 364, 767, 844, 1044
BmrFI CCNGG 4 cut(s) 88, 360, 678, 763
BmrI ACTGGG 1 cut(s) 1386
BmsI GCATC 2 cut(s) 217, 611
BmuI ACTGGG 1 cut(s) 1386
Bpu14I TTCGAA 1 cut(s) 908
BpuMI CCSGG 1 cut(s) 360
BsaAI YACGTR 1 cut(s) 1190
BsaHI GRCGYC 1 cut(s) 1236
BsaJI CCNNGG 4 cut(s) 246, 676, 846, 1038
BsaWI WCCGGW 1 cut(s) 839
Bsc4I CCNNNNNNNGG 5 cut(s) 93, 151, 152, 762, 1381
Bse1I ACTGG 2 cut(s) 1009, 1392
Bse3DI GCAATG 2 cut(s) 415, 1464
BseBI CCWGG 3 cut(s) 88, 678, 763
BseDI CCNNGG 4 cut(s) 246, 676, 846, 1038
BseGI GGATG 3 cut(s) 602, 857, 1370
BseLI CCNNNNNNNGG 5 cut(s) 93, 151, 152, 762, 1381
BseMI GCAATG 2 cut(s) 415, 1464
BseMII CTCAG 3 cut(s) 678, 982, 1229
BseNI ACTGG 2 cut(s) 1009, 1392
BseSI GKGCMC 1 cut(s) 740
BseXI GCAGC 2 cut(s) 523, 809
BsgI GTGCAG 2 cut(s) 143, 733
BshNI GGYRCC 3 cut(s) 67, 96, 362
BsiSI CCGG 2 cut(s) 359, 840
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 5 cut(s) 93, 151, 152, 762, 1381
BsmAI GTCTC 4 cut(s) 327, 881, 1017, 1243
BsmBI CGTCTC 1 cut(s) 1243
BsmFI GGGAC 1 cut(s) 127
BsmI GAATGC 2 cut(s) 900, 1413
Bsp119I TTCGAA 1 cut(s) 908
Bsp1286I GDGCHC 1 cut(s) 740
Bsp143I GATC 5 cut(s) 340, 382, 520, 1069, 1162
Bsp19I CCATGG 3 cut(s) 246, 846, 1038
BspACI CCGC 5 cut(s) 166, 171, 279, 733, 1257
BspCNI CTCAG 3 cut(s) 679, 981, 1228
BspLI GGNNCC 6 cut(s) 69, 98, 364, 767, 844, 1044
BspMI ACCTGC 2 cut(s) 267, 1177
BspPI GGATC 1 cut(s) 1157
BspT104I TTCGAA 1 cut(s) 908
BspT107I GGYRCC 3 cut(s) 67, 96, 362
BsrDI GCAATG 2 cut(s) 415, 1464
BsrI ACTGG 2 cut(s) 1009, 1392
BssECI CCNNGG 4 cut(s) 246, 676, 846, 1038
BssMI GATC 5 cut(s) 340, 382, 520, 1069, 1162
BssNI GRCGYC 1 cut(s) 1236
BssT1I CCWWGG 3 cut(s) 246, 846, 1038
Bst2UI CCWGG 3 cut(s) 88, 678, 763
Bst4CI ACNGT 4 cut(s) 193, 616, 648, 793
BstACI GRCGYC 1 cut(s) 1236
BstAPI GCANNNNNTGC 1 cut(s) 803
BstBAI YACGTR 1 cut(s) 1190
BstBI TTCGAA 1 cut(s) 908
BstC8I GCNNGC 3 cut(s) 716, 740, 1195
BstDEI CTNAG 3 cut(s) 687, 968, 1215
BstDSI CCRYGG 3 cut(s) 246, 846, 1038
BstF5I GGATG 3 cut(s) 602, 857, 1370
BstHHI GCGC 2 cut(s) 139, 1269
BstKTI GATC 5 cut(s) 343, 385, 523, 1072, 1165
BstMAI GTCTC 4 cut(s) 327, 881, 1017, 1243
BstMBI GATC 5 cut(s) 340, 382, 520, 1069, 1162
BstMWI GCNNNNNNNGC 5 cut(s) 542, 739, 803, 1366, 1417
BstNI CCWGG 3 cut(s) 88, 678, 763
BstNSI RCATGY 1 cut(s) 1197
BstSCI CCNGG 4 cut(s) 86, 358, 676, 761
BstSLI GKGCMC 1 cut(s) 740
BstV1I GCAGC 2 cut(s) 523, 809
BstX2I RGATCY 2 cut(s) 1069, 1162
BstYI RGATCY 2 cut(s) 1069, 1162
BsuI GTATCC 2 cut(s) 992, 1118
BtgI CCRYGG 3 cut(s) 246, 846, 1038
BtsCI GGATG 3 cut(s) 602, 857, 1370
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 3 cut(s) 366, 378, 1399
BveI ACCTGC 2 cut(s) 267, 1177
Cac8I GCNNGC 3 cut(s) 716, 740, 1195
CaiI CAGNNNCTG 1 cut(s) 803
CfoI GCGC 2 cut(s) 139, 1269
Cfr13I GGNCC 1 cut(s) 312
Csp6I GTAC 2 cut(s) 394, 1202
CviAII CATG 9 cut(s) 72, 76, 242, 247, 515, 847, 1039, 1194, 1402
CviJI RGCY 8 cut(s) 358, 497, 545, 702, 755, 800, 1369, 1420
CviKI_1 RGCY 8 cut(s) 358, 497, 545, 702, 755, 800, 1369, 1420
CviQI GTAC 2 cut(s) 394, 1202
DdeI CTNAG 3 cut(s) 687, 968, 1215
DpnI GATC 5 cut(s) 342, 384, 522, 1071, 1164
DpnII GATC 5 cut(s) 340, 382, 520, 1069, 1162
Eco130I CCWWGG 3 cut(s) 246, 846, 1038
Eco47I GGWCC 1 cut(s) 312
Eco57I CTGAAG 2 cut(s) 559, 1050
EcoRII CCWGG 3 cut(s) 86, 676, 761
EcoT14I CCWWGG 3 cut(s) 246, 846, 1038
EcoT22I ATGCAT 3 cut(s) 77, 779, 1413
ErhI CCWWGG 3 cut(s) 246, 846, 1038
Esp3I CGTCTC 1 cut(s) 1243
FaeI CATG 9 cut(s) 75, 79, 245, 250, 518, 850, 1042, 1197, 1405
FalI AAGNNNNNCTT 2 cut(s) 1048, 1080
FaqI GGGAC 1 cut(s) 127
FatI CATG 9 cut(s) 71, 75, 241, 246, 514, 846, 1038, 1193, 1401
FauI CCCGC 1 cut(s) 726
FbaI TGATCA 1 cut(s) 520
FblI GTMKAC 2 cut(s) 43, 195
Fnu4HI GCNGC 2 cut(s) 537, 798
FokI GGATG 3 cut(s) 589, 864, 1357
Fsp4HI GCNGC 2 cut(s) 537, 798
FspBI CTAG 1 cut(s) 1016
GlaI GCGC 2 cut(s) 138, 1268
GluI GCNGC 2 cut(s) 537, 798
HapII CCGG 2 cut(s) 359, 840
HhaI GCGC 2 cut(s) 139, 1269
Hin1I GRCGYC 1 cut(s) 1236
Hin1II CATG 9 cut(s) 75, 79, 245, 250, 518, 850, 1042, 1197, 1405
Hin6I GCGC 2 cut(s) 137, 1267
HinP1I GCGC 2 cut(s) 137, 1267
HinfI GANTC 9 cut(s) 302, 374, 560, 693, 950, 964, 1005, 1034, 1319
HpaII CCGG 2 cut(s) 359, 840
HphI GGTGA 1 cut(s) 1328
Hpy166II GTNNAC 4 cut(s) 44, 196, 505, 727
Hpy188I TCNGA 6 cut(s) 55, 559, 578, 637, 971, 1069
Hpy188III TCNNGA 3 cut(s) 386, 824, 1180
Hpy8I GTNNAC 4 cut(s) 44, 196, 505, 727
HpyAV CCTTC 3 cut(s) 52, 564, 904
HpyCH4III ACNGT 4 cut(s) 193, 616, 648, 793
HpyCH4IV ACGT 2 cut(s) 1189, 1236
HpyF10VI GCNNNNNNNGC 5 cut(s) 542, 739, 803, 1366, 1417
HpyF3I CTNAG 3 cut(s) 687, 968, 1215
HpySE526I ACGT 2 cut(s) 1189, 1236
Hsp92I GRCGYC 1 cut(s) 1236
Hsp92II CATG 9 cut(s) 75, 79, 245, 250, 518, 850, 1042, 1197, 1405
HspAI GCGC 2 cut(s) 137, 1267
Ksp22I TGATCA 1 cut(s) 520
Kzo9I GATC 5 cut(s) 340, 382, 520, 1069, 1162
LmnI GCTCC 1 cut(s) 707
Lsp1109I GCAGC 2 cut(s) 523, 809
LweI GCATC 2 cut(s) 217, 611
MaeI CTAG 1 cut(s) 1016
MaeII ACGT 2 cut(s) 1189, 1236
MalI GATC 5 cut(s) 342, 384, 522, 1071, 1164
MboI GATC 5 cut(s) 340, 382, 520, 1069, 1162
MboII GAAGA 3 cut(s) 154, 263, 1322
MflI RGATCY 2 cut(s) 1069, 1162
MhlI GDGCHC 1 cut(s) 740
MluCI AATT 9 cut(s) 217, 235, 287, 588, 669, 904, 1133, 1275, 1436
MmeI TCCRAC 1 cut(s) 1085
MnlI CCTC 6 cut(s) 10, 156, 275, 682, 794, 1221
Mph1103I ATGCAT 3 cut(s) 77, 779, 1413
MroXI GAANNNNTTC 1 cut(s) 963
MseI TTAA 4 cut(s) 425, 1116, 1280, 1341
MslI CAYNNNNRTG 3 cut(s) 80, 531, 1406
MspI CCGG 2 cut(s) 359, 840
MspR9I CCNGG 4 cut(s) 88, 360, 678, 763
Mva1269I GAATGC 2 cut(s) 900, 1413
MvaI CCWGG 3 cut(s) 88, 678, 763
MwoI GCNNNNNNNGC 5 cut(s) 542, 739, 803, 1366, 1417
NciI CCSGG 1 cut(s) 360
NcoI CCATGG 3 cut(s) 246, 846, 1038
NdeII GATC 5 cut(s) 340, 382, 520, 1069, 1162
NlaIII CATG 9 cut(s) 75, 79, 245, 250, 518, 850, 1042, 1197, 1405
NlaIV GGNNCC 6 cut(s) 69, 98, 364, 767, 844, 1044
NsiI ATGCAT 3 cut(s) 77, 779, 1413
NspI RCATGY 1 cut(s) 1197
NspV TTCGAA 1 cut(s) 908
PaeI GCATGC 1 cut(s) 1197
PaqCI CACCTGC 1 cut(s) 1177
PctI GAATGC 2 cut(s) 900, 1413
PdmI GAANNNNTTC 1 cut(s) 963
PfeI GAWTC 9 cut(s) 302, 374, 560, 693, 950, 964, 1005, 1034, 1319
PflFI GACNNNGTC 1 cut(s) 660
PflMI CCANNNNNTGG 1 cut(s) 93
PfoI TCCNGGA 1 cut(s) 761
PkrI GCNGC 2 cut(s) 538, 799
Ppu21I YACGTR 1 cut(s) 1190
PsiI TTATAA 1 cut(s) 294
Psp6I CCWGG 3 cut(s) 86, 676, 761
PspGI CCWGG 3 cut(s) 86, 676, 761
PspN4I GGNNCC 6 cut(s) 69, 98, 364, 767, 844, 1044
PspPI GGNCC 1 cut(s) 312
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 2 cut(s) 1069, 1162
PsyI GACNNNGTC 1 cut(s) 660
RsaI GTAC 2 cut(s) 395, 1203
RsaNI GTAC 2 cut(s) 394, 1202
RseI CAYNNNNRTG 3 cut(s) 80, 531, 1406
SaqAI TTAA 4 cut(s) 425, 1116, 1280, 1341
SatI GCNGC 2 cut(s) 537, 798
Sau3AI GATC 5 cut(s) 340, 382, 520, 1069, 1162
Sau96I GGNCC 1 cut(s) 312
ScrFI CCNGG 4 cut(s) 88, 360, 678, 763
SduI GDGCHC 1 cut(s) 740
SfaNI GCATC 2 cut(s) 217, 611
SfuI TTCGAA 1 cut(s) 908
SinI GGWCC 1 cut(s) 312
SmiMI CAYNNNNRTG 3 cut(s) 80, 531, 1406
SphI GCATGC 1 cut(s) 1197
Sse9I AATT 9 cut(s) 217, 235, 287, 588, 669, 904, 1133, 1275, 1436
SsiI CCGC 5 cut(s) 166, 171, 279, 733, 1257
SspMI CTAG 1 cut(s) 1016
StyD4I CCNGG 4 cut(s) 86, 358, 676, 761
StyI CCWWGG 3 cut(s) 246, 846, 1038
TaaI ACNGT 4 cut(s) 193, 616, 648, 793
TaiI ACGT 2 cut(s) 1192, 1239
TaqI TCGA 5 cut(s) 343, 655, 866, 900, 908
TasI AATT 9 cut(s) 217, 235, 287, 588, 669, 904, 1133, 1275, 1436
TfiI GAWTC 9 cut(s) 302, 374, 560, 693, 950, 964, 1005, 1034, 1319
Tru1I TTAA 4 cut(s) 425, 1116, 1280, 1341
Tru9I TTAA 4 cut(s) 425, 1116, 1280, 1341
TscAI CASTG 3 cut(s) 373, 385, 1399
TseI GCWGC 2 cut(s) 536, 797
TspDTI ATGAA 7 cut(s) 230, 443, 846, 884, 902, 963, 1145
TspGWI ACGGA 1 cut(s) 1017
TspRI CASTG 3 cut(s) 373, 385, 1399
Tth111I GACNNNGTC 1 cut(s) 660
Van91I CCANNNNNTGG 1 cut(s) 93
VpaK11BI GGWCC 1 cut(s) 312
XapI RAATTY 2 cut(s) 1133, 1275
XceI RCATGY 1 cut(s) 1197
XcmI CCANNNNNNNNNTGG 1 cut(s) 1399
XmiI GTMKAC 2 cut(s) 43, 195
XmnI GAANNNNTTC 1 cut(s) 963
XspI CTAG 1 cut(s) 1016
ZraI GACGTC 1 cut(s) 1237
Zsp2I ATGCAT 3 cut(s) 77, 779, 1413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.