Rroxscaffold_2G00135040

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007417
Physical Location & Seq
Forward (+)
72676998 .. 72680703
3706 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00135040.1

Sequence Viewer

Length: 1377 bp
ATGCAGAGATTGCACCCTCGTGAAGTTTCCAAGCCCTGCAGGGCTGCAGGGTTAACAATTCCATGCAAGGTTGACAGTGCAAACAAGCACGGGATTTTACTGGAGATGGTCCAAGTTTTGACAGATCTTGATCTTGTTATTTCCAAATCTTATATCTCCTCTGATGGTGGATGGTTTATGGATGTGTTCCATGTGACGGATCAACTCGGCAACAAAATCACTGACGAGAGCATCATACTTTATATTCAGCAGGCACTTTGTGCTAAGAGGGGAGTGATTGCAAAAGAGGCAAGAATATGCTTAGGAAAAGAAGTGGCACCGGGAGCGAAAGATTTAGCCCTAGAAATGACCACCAAGGACCGACCGGGCTTAATGTCCGAGATCTCCGCGGTCCTGGTTGAGCTCGGCTGCAACATAACTGCTGCCGTGGCTTGGACCCACAGCACCCGAGCGGCGAGTATCTTCTATGTCGAAGATGGGCTGGACGGAGGGCCCATCACGGACCCAATACGGCTAGCAAATGTCCAAGAGCAGCTAGAAAATGTAATTGAAGCCCATCACGAGGAGGGCGAGAAGAGGAGTGTGAGGCTTACAACCCCCGCGGTCGGACGCACCCACACGGAGCGGCGGCTTCACCAATTGATGTACGGGGATGATGACTACGAGATGTGTCAGGGTTGCGATGGCGGTGATAAAGGACACAAGAAGGGTTGTGACCGAACCCATGTGTCGATAGAGAGTTGCAAAGAGAAAGGGTATTGGATTGTCAATGTAAGAAGCAAAGACCGTCCTAAGCTGTTGTTTGACACGGTTTGTGCTCTCACTGACTTGGAATACGTTGTGTTCCATGCTCTGGTTTCTTCCAGGGGCACCATGGCTGATCAGGAATATTTTGTAAGACACAAGGATGGCTACACTCTGAACGCAGAGAGTGAAAGGCAAAAGCTCACTCTATGTTTAGTTGCTGCAATAGAGAGAAGGATCTCAGAAGGATTGAGACTTGACCTCTGTGCCAAAAACCGGATGGGACTATTGTCAGACATCACAAGGGTTTTTCGAGAGAGTGGTTTATCAATATCAAGAGTCGATGTTGGAACATATGGTGAGAGAGCAGTGGGATCAATCTATGTCACAGATGCTTCAGGTCACGATGTGAATCTGAACACAGTGGAATTGGTGACAAATAAGATTGGAGGATCTGCCATTGCGGTTCACAAATCACAGAAGTGGGCTGCTCATGGTTCTTCCTCAAGGACAAGCCAGAGCGTACCAAACACGAGAGTAGAAGATGAAGGAAAACCAAGATCGTCATTTGGAAGTTTGCTCTGGGCTCAACTGGAGCGCCTTTCAAGCAGCTTTGGACCCATTAGATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0016597 GO:0031406 GO:0036094 GO:0043167 GO:0043168 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT ACT_6

Protein Analysis

458

Amino Acids

50.47

Weight (kDa)

6.58

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 114 - 163 7.5e-07 ACT domain
ACT PF01842 337 - 385 6e-06 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 316, 869
AccB7I CCANNNNNTGG 1 cut(s) 853
AccBSI CCGCTC 2 cut(s) 452, 625
AccII CGCG 2 cut(s) 389, 602
AciI CCGC 9 cut(s) 387, 389, 452, 600, 602, 625, 628, 687, 1208
AclWI GGATC 4 cut(s) 207, 989, 1126, 1204
AcuI CTGAAG 1 cut(s) 1125
AdeI CACNNNGTG 2 cut(s) 260, 1153
AfaI GTAC 2 cut(s) 647, 1269
AfiI CCNNNNNNNGG 6 cut(s) 196, 432, 562, 605, 853, 1020
AgsI TTSAA 2 cut(s) 551, 1350
AjnI CCWGG 2 cut(s) 393, 863
AleI CACNNNNGTG 2 cut(s) 18, 1225
AluBI AGCT 5 cut(s) 403, 535, 796, 946, 1356
AluI AGCT 5 cut(s) 403, 535, 796, 946, 1356
Alw21I GWGCWC 2 cut(s) 405, 820
Alw26I GTCTC 1 cut(s) 991
AlwI GGATC 4 cut(s) 207, 989, 1126, 1204
Ama87I CYCGRG 1 cut(s) 447
AoxI GGCC 1 cut(s) 491
ApaI GGGCCC 1 cut(s) 495
ApeKI GCWGC 7 cut(s) 44, 408, 422, 532, 965, 1232, 1353
AspLEI GCGC 1 cut(s) 1344
AspS9I GGNCC 8 cut(s) 109, 358, 391, 435, 491, 492, 502, 1361
AsuC2I CCSGG 2 cut(s) 321, 366
AsuHPI GGTGA 4 cut(s) 626, 701, 1115, 1189
AsuNHI GCTAGC 1 cut(s) 514
AvaI CYCGRG 1 cut(s) 447
AvaII GGWCC 6 cut(s) 109, 358, 391, 435, 502, 1361
BaeGI GKGCMC 2 cut(s) 495, 872
BanI GGYRCC 2 cut(s) 316, 869
BanII GRGCYC 3 cut(s) 405, 495, 1333
BauI CACGAG 3 cut(s) 18, 560, 1276
Bbv12I GWGCWC 2 cut(s) 405, 820
BbvI GCAGC 7 cut(s) 31, 395, 409, 544, 952, 1219, 1365
BccI CCATC 9 cut(s) 100, 158, 165, 470, 503, 564, 677, 902, 1018
BceAI ACGGC 2 cut(s) 410, 527
BciT130I CCWGG 2 cut(s) 395, 865
BclI TGATCA 1 cut(s) 880
BcnI CCSGG 2 cut(s) 321, 366
BcoDI GTCTC 1 cut(s) 991
BfaI CTAG 3 cut(s) 341, 515, 536
BfmI CTRYAG 2 cut(s) 37, 45
BfoI RGCGCY 1 cut(s) 1345
BglII AGATCT 2 cut(s) 124, 381
Bme1390I CCNGG 4 cut(s) 321, 366, 395, 865
Bme18I GGWCC 6 cut(s) 109, 358, 391, 435, 502, 1361
BmeT110I CYCGRG 1 cut(s) 447
BmgT120I GGNCC 8 cut(s) 109, 358, 391, 435, 491, 492, 502, 1361
BmiI GGNNCC 6 cut(s) 318, 437, 493, 504, 871, 1363
BmrFI CCNGG 4 cut(s) 321, 366, 395, 865
BmsI GCATC 2 cut(s) 240, 1126
BmtI GCTAGC 1 cut(s) 518
BoxI GACNNNNGTC 1 cut(s) 1033
BpmI CTGGAG 2 cut(s) 122, 1358
Bpu10I CCTNAGC 2 cut(s) 301, 792
BpuEI CTTGAG 1 cut(s) 1234
BpuMI CCSGG 2 cut(s) 321, 366
BsaBI GATNNNNATC 2 cut(s) 129, 1155
BsaJI CCNNGG 6 cut(s) 354, 387, 426, 600, 864, 873
BsaWI WCCGGW 1 cut(s) 1020
Bsc4I CCNNNNNNNGG 6 cut(s) 196, 432, 562, 605, 853, 1020
Bse1I ACTGG 2 cut(s) 105, 1341
Bse3DI GCAATG 1 cut(s) 1203
Bse8I GATNNNNATC 2 cut(s) 129, 1155
BseBI CCWGG 2 cut(s) 395, 865
BseDI CCNNGG 6 cut(s) 354, 387, 426, 600, 864, 873
BseGI GGATG 5 cut(s) 176, 187, 658, 913, 1029
BseJI GATNNNNATC 2 cut(s) 129, 1155
BseLI CCNNNNNNNGG 6 cut(s) 196, 432, 562, 605, 853, 1020
BseMI GCAATG 1 cut(s) 1203
BseMII CTCAG 1 cut(s) 999
BseNI ACTGG 2 cut(s) 105, 1341
BseRI GAGGAG 3 cut(s) 148, 578, 592
BseSI GKGCMC 2 cut(s) 495, 872
BseXI GCAGC 7 cut(s) 31, 395, 409, 544, 952, 1219, 1365
Bsh1236I CGCG 2 cut(s) 389, 602
Bsh1285I CGRYCG 2 cut(s) 365, 606
BshFI GGCC 1 cut(s) 493
BshNI GGYRCC 2 cut(s) 316, 869
BsiEI CGRYCG 2 cut(s) 365, 606
BsiHKAI GWGCWC 2 cut(s) 405, 820
BsiHKCI CYCGRG 1 cut(s) 447
BsiSI CCGG 3 cut(s) 320, 365, 1021
BslFI GGGAC 1 cut(s) 1041
BslI CCNNNNNNNGG 6 cut(s) 196, 432, 562, 605, 853, 1020
BsmAI GTCTC 1 cut(s) 991
BsmFI GGGAC 1 cut(s) 1041
BsnI GGCC 1 cut(s) 493
BsoBI CYCGRG 1 cut(s) 447
Bsp120I GGGCCC 1 cut(s) 491
Bsp1286I GDGCHC 5 cut(s) 405, 495, 820, 872, 1333
Bsp19I CCATGG 1 cut(s) 873
BspACI CCGC 9 cut(s) 387, 389, 452, 600, 602, 625, 628, 687, 1208
BspANI GGCC 1 cut(s) 493
BspCNI CTCAG 1 cut(s) 998
BspFNI CGCG 2 cut(s) 389, 602
BspLI GGNNCC 6 cut(s) 318, 437, 493, 504, 871, 1363
BspMAI CTGCAG 2 cut(s) 41, 49
BspOI GCTAGC 1 cut(s) 518
BspPI GGATC 4 cut(s) 207, 989, 1126, 1204
BspT107I GGYRCC 2 cut(s) 316, 869
BsrBI CCGCTC 2 cut(s) 452, 625
BsrDI GCAATG 1 cut(s) 1203
BsrI ACTGG 2 cut(s) 105, 1341
BssECI CCNNGG 6 cut(s) 354, 387, 426, 600, 864, 873
BssSI CACGAG 3 cut(s) 18, 560, 1276
BssT1I CCWWGG 2 cut(s) 354, 873
Bst2BI CACGAG 3 cut(s) 18, 560, 1276
Bst2UI CCWGG 2 cut(s) 395, 865
Bst4CI ACNGT 4 cut(s) 77, 788, 811, 1168
Bst6I CTCTTC 1 cut(s) 569
BstAPI GCANNNNNTGC 2 cut(s) 10, 260
BstC8I GCNNGC 2 cut(s) 252, 516
BstDEI CTNAG 4 cut(s) 264, 301, 792, 985
BstDSI CCRYGG 4 cut(s) 387, 426, 600, 873
BstF5I GGATG 5 cut(s) 176, 187, 658, 913, 1029
BstFNI CGCG 2 cut(s) 389, 602
BstH2I RGCGCY 1 cut(s) 1345
BstHHI GCGC 1 cut(s) 1344
BstMAI GTCTC 1 cut(s) 991
BstMCI CGRYCG 2 cut(s) 365, 606
BstMWI GCNNNNNNNGC 6 cut(s) 10, 260, 287, 323, 428, 1350
BstNI CCWGG 2 cut(s) 395, 865
BstPAI GACNNNNGTC 1 cut(s) 1033
BstSCI CCNGG 4 cut(s) 319, 364, 393, 863
BstSFI CTRYAG 2 cut(s) 37, 45
BstSLI GKGCMC 2 cut(s) 495, 872
BstUI CGCG 2 cut(s) 389, 602
BstV1I GCAGC 7 cut(s) 31, 395, 409, 544, 952, 1219, 1365
BstX2I RGATCY 4 cut(s) 124, 381, 981, 1196
BstYI RGATCY 4 cut(s) 124, 381, 981, 1196
BsuRI GGCC 1 cut(s) 493
BtgI CCRYGG 4 cut(s) 387, 426, 600, 873
BtgZI GCGATG 1 cut(s) 696
BtsCI GGATG 5 cut(s) 176, 187, 658, 913, 1029
BtsI GCAGTG 1 cut(s) 1119
BtsIMutI CAGTG 5 cut(s) 82, 219, 822, 1119, 1173
Cac8I GCNNGC 2 cut(s) 252, 516
CfoI GCGC 1 cut(s) 1344
Cfr13I GGNCC 8 cut(s) 109, 358, 391, 435, 491, 492, 502, 1361
Cfr42I CCGCGG 2 cut(s) 390, 603
CseI GACGC 1 cut(s) 618
Csp6I GTAC 2 cut(s) 646, 1268
CviAII CATG 6 cut(s) 63, 191, 725, 848, 874, 1238
CviQI GTAC 2 cut(s) 646, 1268
DdeI CTNAG 4 cut(s) 264, 301, 792, 985
DraIII CACNNNGTG 2 cut(s) 260, 1153
Eam1104I CTCTTC 1 cut(s) 569
EarI CTCTTC 1 cut(s) 569
Ecl136II GAGCTC 1 cut(s) 403
Eco130I CCWWGG 2 cut(s) 354, 873
Eco24I GRGCYC 3 cut(s) 405, 495, 1333
Eco47I GGWCC 6 cut(s) 109, 358, 391, 435, 502, 1361
Eco53kI GAGCTC 1 cut(s) 403
Eco57I CTGAAG 1 cut(s) 1125
Eco88I CYCGRG 1 cut(s) 447
EcoICRI GAGCTC 1 cut(s) 403
EcoO109I RGGNCCY 1 cut(s) 491
EcoRII CCWGG 2 cut(s) 393, 863
EcoT14I CCWWGG 2 cut(s) 354, 873
EcoT38I GRGCYC 3 cut(s) 405, 495, 1333
ErhI CCWWGG 2 cut(s) 354, 873
FaeI CATG 6 cut(s) 66, 194, 728, 851, 877, 1241
FaqI GGGAC 1 cut(s) 1041
FatI CATG 6 cut(s) 62, 190, 724, 847, 873, 1237
FauI CCCGC 1 cut(s) 607
FauNDI CATATG 1 cut(s) 1099
FbaI TGATCA 1 cut(s) 880
FokI GGATG 5 cut(s) 183, 194, 665, 920, 1036
FriOI GRGCYC 3 cut(s) 405, 495, 1333
FspBI CTAG 3 cut(s) 341, 515, 536
GlaI GCGC 1 cut(s) 1343
GsuI CTGGAG 2 cut(s) 122, 1358
HaeII RGCGCY 1 cut(s) 1345
HaeIII GGCC 1 cut(s) 493
HapII CCGG 3 cut(s) 320, 365, 1021
HgaI GACGC 1 cut(s) 618
HhaI GCGC 1 cut(s) 1344
Hin1II CATG 6 cut(s) 66, 194, 728, 851, 877, 1241
Hin6I GCGC 1 cut(s) 1342
HinP1I GCGC 1 cut(s) 1342
HincII GTYRAC 2 cut(s) 54, 73
HindII GTYRAC 2 cut(s) 54, 73
HinfI GANTC 2 cut(s) 1083, 1156
HpaI GTTAAC 1 cut(s) 54
HpaII CCGG 3 cut(s) 320, 365, 1021
HphI GGTGA 4 cut(s) 626, 701, 1115, 1189
Hpy166II GTNNAC 3 cut(s) 54, 73, 1213
Hpy188I TCNGA 7 cut(s) 163, 379, 608, 921, 988, 1039, 1161
Hpy188III TCNNGA 7 cut(s) 20, 128, 560, 884, 1058, 1080, 1148
Hpy8I GTNNAC 3 cut(s) 54, 73, 1213
HpyAV CCTTC 4 cut(s) 700, 972, 983, 1286
HpyCH4III ACNGT 4 cut(s) 77, 788, 811, 1168
HpyCH4IV ACGT 1 cut(s) 837
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 260, 287, 323, 428, 1350
HpyF3I CTNAG 4 cut(s) 264, 301, 792, 985
HpySE526I ACGT 1 cut(s) 837
Hsp92II CATG 6 cut(s) 66, 194, 728, 851, 877, 1241
HspAI GCGC 1 cut(s) 1342
Ksp22I TGATCA 1 cut(s) 880
KspAI GTTAAC 1 cut(s) 54
KspI CCGCGG 2 cut(s) 390, 603
LmnI GCTCC 3 cut(s) 323, 622, 1339
Lsp1109I GCAGC 7 cut(s) 31, 395, 409, 544, 952, 1219, 1365
LweI GCATC 2 cut(s) 240, 1126
MaeI CTAG 3 cut(s) 341, 515, 536
MaeII ACGT 1 cut(s) 837
MaeIII GTNAC 5 cut(s) 193, 713, 1129, 1145, 1177
MbiI CCGCTC 2 cut(s) 452, 625
MboII GAAGA 6 cut(s) 454, 485, 586, 852, 1236, 1298
MfeI CAATTG 1 cut(s) 638
MflI RGATCY 4 cut(s) 124, 381, 981, 1196
MhlI GDGCHC 5 cut(s) 405, 495, 820, 872, 1333
MluCI AATT 4 cut(s) 57, 546, 638, 1172
MlyI GAGTC 1 cut(s) 1092
MmeI TCCRAC 2 cut(s) 586, 1072
MseI TTAA 2 cut(s) 53, 371
MslI CAYNNNNRTG 3 cut(s) 18, 906, 1225
MspA1I CMGCKG 2 cut(s) 389, 602
MspI CCGG 3 cut(s) 320, 365, 1021
MspR9I CCNGG 4 cut(s) 321, 366, 395, 865
MunI CAATTG 1 cut(s) 638
MvaI CCWGG 2 cut(s) 395, 865
MvnI CGCG 2 cut(s) 389, 602
MwoI GCNNNNNNNGC 6 cut(s) 10, 260, 287, 323, 428, 1350
NciI CCSGG 2 cut(s) 321, 366
NcoI CCATGG 1 cut(s) 873
NdeI CATATG 1 cut(s) 1099
NheI GCTAGC 1 cut(s) 514
NlaIII CATG 6 cut(s) 66, 194, 728, 851, 877, 1241
NlaIV GGNNCC 6 cut(s) 318, 437, 493, 504, 871, 1363
NmeAIII GCCGAG 2 cut(s) 186, 384
NmuCI GTSAC 5 cut(s) 193, 713, 1129, 1145, 1177
OliI CACNNNNGTG 2 cut(s) 18, 1225
PcsI WCGNNNNNNNCGW 1 cut(s) 567
PfeI GAWTC 1 cut(s) 1156
PflMI CCANNNNNTGG 1 cut(s) 853
PleI GAGTC 1 cut(s) 1091
PpsI GAGTC 1 cut(s) 1091
PshAI GACNNNNGTC 1 cut(s) 1033
Psp124BI GAGCTC 1 cut(s) 405
Psp6I CCWGG 2 cut(s) 393, 863
PspGI CCWGG 2 cut(s) 393, 863
PspN4I GGNNCC 6 cut(s) 318, 437, 493, 504, 871, 1363
PspOMI GGGCCC 1 cut(s) 491
PspPI GGNCC 8 cut(s) 109, 358, 391, 435, 491, 492, 502, 1361
PstI CTGCAG 2 cut(s) 41, 49
PsuI RGATCY 4 cut(s) 124, 381, 981, 1196
RsaI GTAC 2 cut(s) 647, 1269
RsaNI GTAC 2 cut(s) 646, 1268
RseI CAYNNNNRTG 3 cut(s) 18, 906, 1225
SacI GAGCTC 1 cut(s) 405
SacII CCGCGG 2 cut(s) 390, 603
SaqAI TTAA 2 cut(s) 53, 371
Sau96I GGNCC 8 cut(s) 109, 358, 391, 435, 491, 492, 502, 1361
SbfI CCTGCAGG 1 cut(s) 41
SchI GAGTC 1 cut(s) 1092
ScrFI CCNGG 4 cut(s) 321, 366, 395, 865
SdaI CCTGCAGG 1 cut(s) 41
SduI GDGCHC 5 cut(s) 405, 495, 820, 872, 1333
SetI ASST 9 cut(s) 72, 405, 537, 798, 840, 948, 1008, 1147, 1358
SfaNI GCATC 2 cut(s) 240, 1126
SfcI CTRYAG 2 cut(s) 37, 45
Sfr303I CCGCGG 2 cut(s) 390, 603
SgrBI CCGCGG 2 cut(s) 390, 603
SinI GGWCC 6 cut(s) 109, 358, 391, 435, 502, 1361
SmiMI CAYNNNNRTG 3 cut(s) 18, 906, 1225
SmlI CTYRAG 1 cut(s) 1249
SmoI CTYRAG 1 cut(s) 1249
Sse8387I CCTGCAGG 1 cut(s) 41
Sse9I AATT 4 cut(s) 57, 546, 638, 1172
SsiI CCGC 9 cut(s) 387, 389, 452, 600, 602, 625, 628, 687, 1208
SspI AATATT 1 cut(s) 890
SspMI CTAG 3 cut(s) 341, 515, 536
SstI GAGCTC 1 cut(s) 405
StyD4I CCNGG 4 cut(s) 319, 364, 393, 863
StyI CCWWGG 2 cut(s) 354, 873
TaaI ACNGT 4 cut(s) 77, 788, 811, 1168
TaiI ACGT 1 cut(s) 840
TaqI TCGA 4 cut(s) 471, 731, 1057, 1086
TaqII GACCGA 2 cut(s) 375, 732
TasI AATT 4 cut(s) 57, 546, 638, 1172
TauI GCSGC 3 cut(s) 455, 628, 631
TfiI GAWTC 1 cut(s) 1156
Tru1I TTAA 2 cut(s) 53, 371
Tru9I TTAA 2 cut(s) 53, 371
TscAI CASTG 5 cut(s) 82, 226, 829, 1119, 1173
TseFI GTSAC 5 cut(s) 193, 713, 1129, 1145, 1177
TseI GCWGC 7 cut(s) 44, 408, 422, 532, 965, 1232, 1353
Tsp45I GTSAC 5 cut(s) 193, 713, 1129, 1145, 1177
TspDTI ATGAA 1 cut(s) 1305
TspGWI ACGGA 4 cut(s) 212, 501, 515, 635
TspRI CASTG 5 cut(s) 82, 226, 829, 1119, 1173
Van91I CCANNNNNTGG 1 cut(s) 853
VpaK11BI GGWCC 6 cut(s) 109, 358, 391, 435, 502, 1361
XcmI CCANNNNNNNNNTGG 2 cut(s) 871, 1021
XspI CTAG 3 cut(s) 341, 515, 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.