ATCG00280

One of the components of the core complex of photosystem II (PSII). It binds chlorophyll and helps catalyze the primary light-induced photochemical processes of PSII. PSII is a light- driven water plastoquinone oxidoreductase, using light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
Pt
OG0018721
Physical Location & Seq
Forward (+)
33720 .. 35141
1422 bp
Loading structure...
UTR
Exon/CDS
Intron
ATCG00280.1

Sequence Viewer

Length: 1422 bp
ATGAAAACCTTATATTCCCTGAGGAGGTTCTACCACGTGGAAACGCTCTTTAATGGAACTTTAGCTTTAGCTGGTCGTGACCAAGAAACCACCGGTTTCGCTTGGTGGGCCGGGAATGCCCGACTTATCAATTTATCTGGTAAACTTTTGGGAGCTCATGTAGCCCATGCCGGATTAATCGTATTCTGGGCCGGAGCAATGAACTTATTTGAAGTGGCCCATTTTGTACCTGAAAAGCCCATGTATGAACAAGGATTAATTTTACTTCCCCACCTAGCCACTTTAGGATGGGGGGTAGGTCCTGGGGGAGAAGTTATAGACACCTTTCCGTACTTTGTATCTGGAGTACTTCACTTAATTTCTTCTGCAGTTTTGGGCTTTGGCGGTATTTATCATGCACTTCTGGGACCCGAAACTCTTGAAGAATCTTTTCCCTTTTTCGGTTATGTATGGAAAGATAGAAATAAAATGACCACCATTTTGGGTATTCACTTAATTTTGTTAGGTGTAGGTGCCTTTCTTCTAGTATTCAAGGCTCTCTATTTTGGGGGCGTATATGATACCTGGGCTCCGGGAGGGGGGGATGTAAGAAAAATTACAAACTTGACTCTTAGCCCAAGTGTTATATTTGGTTATTTACTAAAATCTCCCTTTGGGGGAGAAGGATGGATTGTTAGTGTGGACGATTTGGAAGATATAATTGGAGGACATGTATGGTTAGGTTCCATTTGTATATTTGGTGGAATCTGGCATATCTTAACCAAGCCTTTTGCATGGGCTCGCCGCGCACTTGTATGGTCTGGGGAGGCTTACTTGTCTTATAGTTTAGCTGCTTTATCTGTTTGTGGTTTCATTGCTTGTTGTTTTGTCTGGTTTAATAATACCGCTTACCCTAGTGAGTTTTACGGACCTACGGGGCCAGAAGCTTCTCAAGCTCAAGCATTTACTTTTCTAGTTAGAGACCAACGTCTTGGAGCTAACGTGGGGTCTGCTCAAGGACCTACAGGTTTAGGTAAATACTTAATGCGTTCTCCGACTGGAGAAGTTATTTTTGGAGGAGAAACAATGCGTTTTTGGGATCTGCGTGCTCCCTGGTTAGAACCTTTAAGGGGTCCTAACGGTTTGGACTTGAGTAGGTTGAAAAAAGACATACAACCTTGGCAAGAACGACGTTCGGCAGAATATATGACTCATGCTCCTTTAGGTTCGTTAAATTCTGTAGGGGGCGTAGCTACTGAGATCAATGCAGTCAATTATGTCTCTCCGAGAAGTTGGTTATCTACCTCTCATTTTGTTCTAGGATTTTTCCTATTCGTGGGTCATTTATGGCACGCGGGAAGGGCTCGGGCAGCGGCAGCGGGATTTGAAAAAGGAATTGATCGTGATTTTGAACCTGTTCTTTCCATGACTCCTCTTAACTAA

Protein Analysis

473

Amino Acids

51.87

Weight (kDa)

6.71

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSII PF00421 31 - 469 6.2e-132 Photosystem II protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018721)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00280
rosa_laevigata RLG00000022754
rosa_multiflora Rmu_sc0006153.1_g000042
rosa_roxburghii Rroxscaffold_7G00181930
rosa_rugosa RorugMtG0000600
rosa_samantha Rh1BG017200 Rh5AG281200 Rh7DG262700
rosa_wichuraiana Rw0G001310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 512
AccII CGCG 2 cut(s) 786, 1334
AciI CCGC 6 cut(s) 384, 784, 885, 1334, 1352, 1358
AclWI GGATC 1 cut(s) 1086
AcsI RAATTY 1 cut(s) 1213
AcvI CACGTG 1 cut(s) 37
AfaI GTAC 3 cut(s) 228, 332, 348
AfiI CCNNNNNNNGG 6 cut(s) 24, 440, 578, 656, 1109, 1315
AflIII ACRYGT 1 cut(s) 709
AgeI ACCGGT 1 cut(s) 92
AgsI TTSAA 6 cut(s) 212, 422, 532, 1141, 1367, 1391
AjnI CCWGG 3 cut(s) 301, 563, 1091
AjuI GAANNNNNNNTTGG 4 cut(s) 684, 716, 1035, 1067
AluBI AGCT 8 cut(s) 65, 71, 155, 830, 926, 935, 977, 1232
AluI AGCT 8 cut(s) 65, 71, 155, 830, 926, 935, 977, 1232
Alw21I GWGCWC 2 cut(s) 157, 1090
Alw26I GTCTC 2 cut(s) 954, 1264
AlwI GGATC 1 cut(s) 1086
Ama87I CYCGRG 1 cut(s) 1344
AoxI GGCC 4 cut(s) 108, 189, 216, 917
ApeKI GCWGC 3 cut(s) 830, 1349, 1355
ApoI RAATTY 1 cut(s) 1213
ArsI GACNNNNNNTTYG 2 cut(s) 463, 495
AseI ATTAAT 2 cut(s) 176, 257
AsiGI ACCGGT 1 cut(s) 92
Asp700I GAANNNNTTC 2 cut(s) 429, 1395
AspLEI GCGC 1 cut(s) 788
AspS9I GGNCC 9 cut(s) 108, 189, 217, 299, 407, 908, 917, 998, 1112
AsuC2I CCSGG 2 cut(s) 112, 573
AvaI CYCGRG 1 cut(s) 1344
AvaII GGWCC 5 cut(s) 299, 407, 908, 998, 1112
AxyI CCTNAGG 1 cut(s) 20
BanI GGYRCC 1 cut(s) 512
BanII GRGCYC 4 cut(s) 157, 571, 781, 1345
BbrPI CACGTG 1 cut(s) 37
Bbv12I GWGCWC 2 cut(s) 157, 1090
BbvI GCAGC 3 cut(s) 817, 1361, 1367
BccI CCATC 2 cut(s) 282, 660
BciT130I CCWGG 3 cut(s) 303, 565, 1093
BcnI CCSGG 2 cut(s) 112, 573
BcoDI GTCTC 2 cut(s) 954, 1264
BfaI CTAG 5 cut(s) 275, 524, 894, 953, 1298
BfmI CTRYAG 3 cut(s) 366, 1002, 1218
BisI GCNGC 5 cut(s) 784, 831, 1350, 1353, 1356
BlsI GCNGC 5 cut(s) 785, 832, 1351, 1354, 1357
BmcAI AGTACT 1 cut(s) 348
Bme1390I CCNGG 5 cut(s) 112, 303, 565, 573, 1093
Bme18I GGWCC 5 cut(s) 299, 407, 908, 998, 1112
BmeT110I CYCGRG 1 cut(s) 1344
BmgT120I GGNCC 9 cut(s) 108, 189, 217, 299, 407, 908, 917, 998, 1112
BmiI GGNNCC 7 cut(s) 408, 409, 514, 570, 724, 918, 1113
BmrFI CCNGG 5 cut(s) 112, 303, 565, 573, 1093
BoxI GACNNNNGTC 1 cut(s) 966
BpmI CTGGAG 2 cut(s) 363, 1059
BpuEI CTTGAG 4 cut(s) 915, 921, 978, 1150
BpuMI CCSGG 2 cut(s) 112, 573
BsaAI YACGTR 1 cut(s) 37
BsaI GGTCTC 1 cut(s) 954
BsaJI CCNNGG 4 cut(s) 302, 564, 1091, 1157
BsaWI WCCGGW 1 cut(s) 92
Bsc4I CCNNNNNNNGG 6 cut(s) 24, 440, 578, 656, 1109, 1315
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 1 cut(s) 1042
Bse21I CCTNAGG 1 cut(s) 20
Bse3DI GCAATG 2 cut(s) 204, 852
BseBI CCWGG 3 cut(s) 303, 565, 1093
BseDI CCNNGG 4 cut(s) 302, 564, 1091, 1157
BseGI GGATG 3 cut(s) 293, 589, 671
BseLI CCNNNNNNNGG 6 cut(s) 24, 440, 578, 656, 1109, 1315
BseMI GCAATG 2 cut(s) 204, 852
BseMII CTCAG 2 cut(s) 11, 1227
BseNI ACTGG 1 cut(s) 1042
BseRI GAGGAG 3 cut(s) 37, 1071, 1401
BseXI GCAGC 3 cut(s) 817, 1361, 1367
Bsh1236I CGCG 2 cut(s) 786, 1334
BshFI GGCC 4 cut(s) 110, 191, 218, 919
BshNI GGYRCC 1 cut(s) 512
BshTI ACCGGT 1 cut(s) 92
BsiHKAI GWGCWC 2 cut(s) 157, 1090
BsiHKCI CYCGRG 1 cut(s) 1344
BsiSI CCGG 5 cut(s) 93, 111, 171, 192, 572
BslFI GGGAC 1 cut(s) 420
BslI CCNNNNNNNGG 6 cut(s) 24, 440, 578, 656, 1109, 1315
BsmAI GTCTC 2 cut(s) 954, 1264
BsmFI GGGAC 1 cut(s) 420
BsmI GAATGC 1 cut(s) 121
BsnI GGCC 4 cut(s) 110, 191, 218, 919
Bso31I GGTCTC 1 cut(s) 954
BsoBI CYCGRG 1 cut(s) 1344
Bsp1286I GDGCHC 5 cut(s) 157, 571, 781, 1090, 1345
Bsp143I GATC 3 cut(s) 1078, 1239, 1378
BspACI CCGC 6 cut(s) 384, 784, 885, 1334, 1352, 1358
BspANI GGCC 4 cut(s) 110, 191, 218, 919
BspCNI CTCAG 2 cut(s) 12, 1228
BspFNI CGCG 2 cut(s) 786, 1334
BspLI GGNNCC 7 cut(s) 408, 409, 514, 570, 724, 918, 1113
BspMAI CTGCAG 1 cut(s) 370
BspPI GGATC 1 cut(s) 1086
BspT107I GGYRCC 1 cut(s) 512
BspTNI GGTCTC 1 cut(s) 954
BsrDI GCAATG 2 cut(s) 204, 852
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 1 cut(s) 1042
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 4 cut(s) 302, 564, 1091, 1157
BssMI GATC 3 cut(s) 1078, 1239, 1378
BssT1I CCWWGG 1 cut(s) 1157
Bst2UI CCWGG 3 cut(s) 303, 565, 1093
Bst4CI ACNGT 1 cut(s) 1121
BstBAI YACGTR 1 cut(s) 37
BstC8I GCNNGC 3 cut(s) 781, 1086, 1332
BstDEI CTNAG 3 cut(s) 20, 611, 1236
BstF5I GGATG 3 cut(s) 293, 589, 671
BstFNI CGCG 2 cut(s) 786, 1334
BstHHI GCGC 1 cut(s) 788
BstKTI GATC 3 cut(s) 1081, 1242, 1381
BstMAI GTCTC 2 cut(s) 954, 1264
BstMBI GATC 3 cut(s) 1078, 1239, 1378
BstMWI GCNNNNNNNGC 8 cut(s) 107, 116, 161, 785, 932, 1340, 1349, 1355
BstNI CCWGG 3 cut(s) 303, 565, 1093
BstNSI RCATGY 1 cut(s) 713
BstPAI GACNNNNGTC 1 cut(s) 966
BstSCI CCNGG 5 cut(s) 110, 301, 563, 571, 1091
BstSFI CTRYAG 3 cut(s) 366, 1002, 1218
BstUI CGCG 2 cut(s) 786, 1334
BstV1I GCAGC 3 cut(s) 817, 1361, 1367
BstX2I RGATCY 1 cut(s) 1078
BstXI CCANNNNNNTGG 2 cut(s) 481, 971
BstYI RGATCY 1 cut(s) 1078
Bsu36I CCTNAGG 1 cut(s) 20
BsuRI GGCC 4 cut(s) 110, 191, 218, 919
BtsCI GGATG 3 cut(s) 293, 589, 671
Cac8I GCNNGC 3 cut(s) 781, 1086, 1332
CfoI GCGC 1 cut(s) 788
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 9 cut(s) 108, 189, 217, 299, 407, 908, 917, 998, 1112
Csp6I GTAC 3 cut(s) 227, 331, 347
CspAI ACCGGT 1 cut(s) 92
CviAII CATG 8 cut(s) 158, 167, 241, 395, 710, 774, 1193, 1405
CviQI GTAC 3 cut(s) 227, 331, 347
DdeI CTNAG 3 cut(s) 20, 611, 1236
DpnI GATC 3 cut(s) 1080, 1241, 1380
DpnII GATC 3 cut(s) 1078, 1239, 1378
Ecl136II GAGCTC 1 cut(s) 155
Eco130I CCWWGG 1 cut(s) 1157
Eco24I GRGCYC 4 cut(s) 157, 571, 781, 1345
Eco31I GGTCTC 1 cut(s) 954
Eco47I GGWCC 5 cut(s) 299, 407, 908, 998, 1112
Eco53kI GAGCTC 1 cut(s) 155
Eco72I CACGTG 1 cut(s) 37
Eco81I CCTNAGG 1 cut(s) 20
Eco88I CYCGRG 1 cut(s) 1344
EcoICRI GAGCTC 1 cut(s) 155
EcoO109I RGGNCCY 4 cut(s) 299, 407, 998, 1112
EcoRII CCWGG 3 cut(s) 301, 563, 1091
EcoT14I CCWWGG 1 cut(s) 1157
EcoT38I GRGCYC 4 cut(s) 157, 571, 781, 1345
ErhI CCWWGG 1 cut(s) 1157
FaeI CATG 8 cut(s) 161, 170, 244, 398, 713, 777, 1196, 1408
FaqI GGGAC 1 cut(s) 420
FatI CATG 8 cut(s) 157, 166, 240, 394, 709, 773, 1192, 1404
FauI CCCGC 2 cut(s) 1327, 1351
Fnu4HI GCNGC 5 cut(s) 784, 831, 1350, 1353, 1356
FokI GGATG 3 cut(s) 300, 596, 678
FriOI GRGCYC 4 cut(s) 157, 571, 781, 1345
Fsp4HI GCNGC 5 cut(s) 784, 831, 1350, 1353, 1356
FspBI CTAG 5 cut(s) 275, 524, 894, 953, 1298
GlaI GCGC 1 cut(s) 787
GluI GCNGC 5 cut(s) 784, 831, 1350, 1353, 1356
GsuI CTGGAG 2 cut(s) 363, 1059
HaeIII GGCC 4 cut(s) 110, 191, 218, 919
HapII CCGG 5 cut(s) 93, 111, 171, 192, 572
HhaI GCGC 1 cut(s) 788
Hin1II CATG 8 cut(s) 161, 170, 244, 398, 713, 777, 1196, 1408
Hin6I GCGC 1 cut(s) 786
HinP1I GCGC 1 cut(s) 786
HindIII AAGCTT 1 cut(s) 924
HinfI GANTC 5 cut(s) 425, 607, 744, 1189, 1408
HpaII CCGG 5 cut(s) 93, 111, 171, 192, 572
Hpy166II GTNNAC 2 cut(s) 143, 682
Hpy188I TCNGA 2 cut(s) 1035, 1266
Hpy188III TCNNGA 4 cut(s) 77, 342, 419, 1382
Hpy8I GTNNAC 2 cut(s) 143, 682
Hpy99I CGWCG 1 cut(s) 1173
HpyAV CCTTC 2 cut(s) 656, 1332
HpyCH4III ACNGT 1 cut(s) 1121
HpyCH4IV ACGT 4 cut(s) 36, 967, 981, 1171
HpyCH4V TGCA 4 cut(s) 368, 398, 773, 1247
HpyF10VI GCNNNNNNNGC 8 cut(s) 107, 116, 161, 785, 932, 1340, 1349, 1355
HpyF3I CTNAG 3 cut(s) 20, 611, 1236
HpySE526I ACGT 4 cut(s) 36, 967, 981, 1171
Hsp92II CATG 8 cut(s) 161, 170, 244, 398, 713, 777, 1196, 1408
HspAI GCGC 1 cut(s) 786
KflI GGGWCCC 1 cut(s) 407
Kzo9I GATC 3 cut(s) 1078, 1239, 1378
LmnI GCTCC 6 cut(s) 152, 194, 574, 974, 1093, 1201
Lsp1109I GCAGC 3 cut(s) 817, 1361, 1367
MaeI CTAG 5 cut(s) 275, 524, 894, 953, 1298
MaeII ACGT 4 cut(s) 36, 967, 981, 1171
MaeIII GTNAC 1 cut(s) 77
MalI GATC 3 cut(s) 1080, 1241, 1380
MboI GATC 3 cut(s) 1078, 1239, 1378
MboII GAAGA 4 cut(s) 354, 434, 512, 704
MflI RGATCY 1 cut(s) 1078
MhlI GDGCHC 5 cut(s) 157, 571, 781, 1090, 1345
MluCI AATT 9 cut(s) 130, 258, 357, 495, 594, 699, 1213, 1252, 1374
MlyI GAGTC 3 cut(s) 601, 1183, 1402
MmeI TCCRAC 1 cut(s) 1058
MnlI CCTC 8 cut(s) 15, 18, 569, 698, 799, 1049, 1294, 1422
MroXI GAANNNNTTC 2 cut(s) 429, 1395
MslI CAYNNNNRTG 1 cut(s) 793
MspA1I CMGCKG 2 cut(s) 1352, 1358
MspI CCGG 5 cut(s) 93, 111, 171, 192, 572
MspR9I CCNGG 5 cut(s) 112, 303, 565, 573, 1093
Mva1269I GAATGC 1 cut(s) 121
MvaI CCWGG 3 cut(s) 303, 565, 1093
MvnI CGCG 2 cut(s) 786, 1334
MwoI GCNNNNNNNGC 8 cut(s) 107, 116, 161, 785, 932, 1340, 1349, 1355
NciI CCSGG 2 cut(s) 112, 573
NdeII GATC 3 cut(s) 1078, 1239, 1378
NlaIII CATG 8 cut(s) 161, 170, 244, 398, 713, 777, 1196, 1408
NlaIV GGNNCC 7 cut(s) 408, 409, 514, 570, 724, 918, 1113
NmuCI GTSAC 1 cut(s) 77
NspI RCATGY 1 cut(s) 713
PciI ACATGT 1 cut(s) 709
PctI GAATGC 1 cut(s) 121
PdmI GAANNNNTTC 2 cut(s) 429, 1395
PfeI GAWTC 2 cut(s) 425, 744
PfoI TCCNGGA 1 cut(s) 571
PinAI ACCGGT 1 cut(s) 92
PkrI GCNGC 5 cut(s) 785, 832, 1351, 1354, 1357
PleI GAGTC 3 cut(s) 601, 1183, 1402
PmaCI CACGTG 1 cut(s) 37
PmlI CACGTG 1 cut(s) 37
PpsI GAGTC 3 cut(s) 601, 1183, 1402
Ppu21I YACGTR 1 cut(s) 37
PpuMI RGGWCCY 4 cut(s) 299, 407, 998, 1112
PscI ACATGT 1 cut(s) 709
PshAI GACNNNNGTC 1 cut(s) 966
PshBI ATTAAT 2 cut(s) 176, 257
Psp124BI GAGCTC 1 cut(s) 157
Psp5II RGGWCCY 4 cut(s) 299, 407, 998, 1112
Psp6I CCWGG 3 cut(s) 301, 563, 1091
PspCI CACGTG 1 cut(s) 37
PspGI CCWGG 3 cut(s) 301, 563, 1091
PspN4I GGNNCC 7 cut(s) 408, 409, 514, 570, 724, 918, 1113
PspPI GGNCC 9 cut(s) 108, 189, 217, 299, 407, 908, 917, 998, 1112
PspPPI RGGWCCY 4 cut(s) 299, 407, 998, 1112
PstI CTGCAG 1 cut(s) 370
PsuI RGATCY 1 cut(s) 1078
RsaI GTAC 3 cut(s) 228, 332, 348
RsaNI GTAC 3 cut(s) 227, 331, 347
RseI CAYNNNNRTG 1 cut(s) 793
SacI GAGCTC 1 cut(s) 157
SatI GCNGC 5 cut(s) 784, 831, 1350, 1353, 1356
Sau3AI GATC 3 cut(s) 1078, 1239, 1378
Sau96I GGNCC 9 cut(s) 108, 189, 217, 299, 407, 908, 917, 998, 1112
ScaI AGTACT 1 cut(s) 348
SchI GAGTC 3 cut(s) 601, 1183, 1402
ScrFI CCNGG 5 cut(s) 112, 303, 565, 573, 1093
SduI GDGCHC 5 cut(s) 157, 571, 781, 1090, 1345
SfcI CTRYAG 3 cut(s) 366, 1002, 1218
SinI GGWCC 5 cut(s) 299, 407, 908, 998, 1112
SmiMI CAYNNNNRTG 1 cut(s) 793
SmlI CTYRAG 4 cut(s) 930, 936, 993, 1129
SmoI CTYRAG 4 cut(s) 930, 936, 993, 1129
Sse9I AATT 9 cut(s) 130, 258, 357, 495, 594, 699, 1213, 1252, 1374
SsiI CCGC 6 cut(s) 384, 784, 885, 1334, 1352, 1358
SspMI CTAG 5 cut(s) 275, 524, 894, 953, 1298
SstI GAGCTC 1 cut(s) 157
StyD4I CCNGG 5 cut(s) 110, 301, 563, 571, 1091
StyI CCWWGG 1 cut(s) 1157
TaaI ACNGT 1 cut(s) 1121
TaiI ACGT 4 cut(s) 39, 970, 984, 1174
TasI AATT 9 cut(s) 130, 258, 357, 495, 594, 699, 1213, 1252, 1374
TatI WGTACW 1 cut(s) 346
TauI GCSGC 2 cut(s) 786, 1355
TfiI GAWTC 2 cut(s) 425, 744
TseFI GTSAC 1 cut(s) 77
TseI GCWGC 3 cut(s) 830, 1349, 1355
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 4 cut(s) 17, 215, 261, 841
TspGWI ACGGA 2 cut(s) 318, 921
VpaK11BI GGWCC 5 cut(s) 299, 407, 908, 998, 1112
VspI ATTAAT 2 cut(s) 176, 257
XapI RAATTY 1 cut(s) 1213
XceI RCATGY 1 cut(s) 713
XmnI GAANNNNTTC 2 cut(s) 429, 1395
XspI CTAG 5 cut(s) 275, 524, 894, 953, 1298
ZrmI AGTACT 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.