FvH4_1g01430

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0016719
Physical Location & Seq
Reverse (-)
724944 .. 728632
3689 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g01430.t2

Sequence Viewer

Length: 693 bp
ATGCCTCGCGCCACTTCGAAAAGAAAAGCGCCGCCGTCGACCTCCGCCTCCGTCACATCCTCCGACGTCGTTTCGACTCGTCCAGGTAAAAGAGGCAAAAAGGATGTAGACAAGATAGGGGACCTATTTGATGTGTACGTTAACAAGAGCCTCGACATGATTGACCCAGAAGGAATTGTGTCTTTTTGTTCGCATTTGGGAGTGGACCATACTGATGTCAGAATCCTGATGCTTGCTTGGAAAATGAAAGCGGAAAAGCAAGGATACTTTTCCAGGGAAGAGTGGCGAAGAGGCTTGAAAGATTTGAACGCTGACACCATAGCTAAATTGAAGAAAGCACTCCCAGGTCTGGAAAAAGAGTTGATGGCCACACCCAAATTTGAAGATTTCTATGCTTTTGCATTTCAATACTGCTTGACAGAAGACAGACAAAAGAGTGTTGATATCGAGACTGTGTGTGAATTACTGAATCTTGTGCTGGGTCCCAAATACCGATCCCAGGTTGATATATTAACCAAGTATCTGAAGGTCCAAAGTGAGTACAGGGCATTAAATGCTGATCAGTGGAGACATTTTTACCGTTTTTGCAAGGAGATAAGTTTCCCAGACCTCGAAGACTATGATTCTGATCAAGCATGGCCTGTGATAATTGATAATTTTGTTGAATGGATGAAAGAAAATAAGCAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

231

Amino Acids

26.64

Weight (kDa)

6.84

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 89 - 203 1.4e-28 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016719)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01430 FvH4_1g01430
malus_domestica MD15G1157200.v1.1
prunus_persica Prupe.7G258300_v2.0.a1 Prupe.7G258300_v2.0.a1
pyrus_communis pycom15g14100
rosa_chinensis RchiOBHm_Chr2g0086341
rosa_laevigata RLG00000015751
rosa_multiflora Rmu_sc0003572.1_g000003 Rmu_sc0004510.1_g000020
rosa_roxburghii Rroxscaffold_2G00154680
rosa_rugosa Rorug01G0466700
rosa_samantha Rh2AG016900
rosa_wichuraiana Rw2G001430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 69
AccI GTMKAC 2 cut(s) 38, 108
AccII CGCG 1 cut(s) 9
AciI CCGC 3 cut(s) 32, 45, 251
AclWI GGATC 1 cut(s) 489
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 1 cut(s) 377
AcuI CTGAAG 1 cut(s) 545
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 2 cut(s) 137, 542
AfiI CCNNNNNNNGG 2 cut(s) 349, 499
AgsI TTSAA 6 cut(s) 298, 307, 331, 383, 407, 665
AjnI CCWGG 4 cut(s) 82, 272, 343, 498
AluBI AGCT 2 cut(s) 323, 690
AluI AGCT 2 cut(s) 323, 690
Alw26I GTCTC 2 cut(s) 443, 562
AlwI GGATC 1 cut(s) 489
AoxI GGCC 2 cut(s) 366, 638
ApoI RAATTY 1 cut(s) 377
AspLEI GCGC 2 cut(s) 11, 31
AspS9I GGNCC 4 cut(s) 121, 205, 482, 529
AsuII TTCGAA 1 cut(s) 17
AvaII GGWCC 4 cut(s) 121, 205, 482, 529
BalI TGGCCA 1 cut(s) 368
BbsI GAAGAC 2 cut(s) 429, 621
BccI CCATC 1 cut(s) 358
BceAI ACGGC 1 cut(s) 19
BciT130I CCWGG 4 cut(s) 84, 274, 345, 500
BciVI GTATCC 1 cut(s) 257
BclI TGATCA 2 cut(s) 559, 628
BcoDI GTCTC 2 cut(s) 443, 562
BfoI RGCGCY 1 cut(s) 32
BfuI GTATCC 1 cut(s) 257
BisI GCNGC 1 cut(s) 32
BlsI GCNGC 1 cut(s) 33
Bme1390I CCNGG 4 cut(s) 84, 274, 345, 500
Bme18I GGWCC 4 cut(s) 121, 205, 482, 529
BmgT120I GGNCC 4 cut(s) 121, 205, 482, 529
BmiI GGNNCC 3 cut(s) 122, 483, 484
BmrFI CCNGG 4 cut(s) 84, 274, 345, 500
BmsI GCATC 1 cut(s) 219
BpiI GAAGAC 2 cut(s) 429, 621
Bpu14I TTCGAA 1 cut(s) 17
BsaBI GATNNNNATC 1 cut(s) 627
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 3 cut(s) 273, 343, 498
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 499
Bse8I GATNNNNATC 1 cut(s) 627
BseBI CCWGG 4 cut(s) 84, 274, 345, 500
BseDI CCNNGG 3 cut(s) 273, 343, 498
BseGI GGATG 3 cut(s) 56, 109, 675
BseJI GATNNNNATC 1 cut(s) 627
BseLI CCNNNNNNNGG 2 cut(s) 349, 499
BseYI CCCAGC 1 cut(s) 478
Bsh1236I CGCG 1 cut(s) 9
BshFI GGCC 2 cut(s) 368, 640
BslFI GGGAC 2 cut(s) 134, 468
BslI CCNNNNNNNGG 2 cut(s) 349, 499
BsmAI GTCTC 2 cut(s) 443, 562
BsmFI GGGAC 2 cut(s) 134, 468
BsnI GGCC 2 cut(s) 368, 640
Bsp119I TTCGAA 1 cut(s) 17
Bsp143I GATC 3 cut(s) 494, 559, 628
BspACI CCGC 3 cut(s) 32, 45, 251
BspANI GGCC 2 cut(s) 368, 640
BspFNI CGCG 1 cut(s) 9
BspLI GGNNCC 3 cut(s) 122, 483, 484
BspPI GGATC 1 cut(s) 489
BspT104I TTCGAA 1 cut(s) 17
BssECI CCNNGG 3 cut(s) 273, 343, 498
BssMI GATC 3 cut(s) 494, 559, 628
BssNI GRCGYC 1 cut(s) 66
Bst2UI CCWGG 4 cut(s) 84, 274, 345, 500
Bst4CI ACNGT 2 cut(s) 454, 581
Bst6I CTCTTC 2 cut(s) 273, 283
BstACI GRCGYC 1 cut(s) 66
BstAPI GCANNNNNTGC 1 cut(s) 554
BstBI TTCGAA 1 cut(s) 17
BstC8I GCNNGC 1 cut(s) 234
BstF5I GGATG 3 cut(s) 56, 109, 675
BstFNI CGCG 1 cut(s) 9
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 2 cut(s) 11, 31
BstKTI GATC 3 cut(s) 497, 562, 631
BstMAI GTCTC 2 cut(s) 443, 562
BstMBI GATC 3 cut(s) 494, 559, 628
BstMWI GCNNNNNNNGC 1 cut(s) 554
BstNI CCWGG 4 cut(s) 84, 274, 345, 500
BstSCI CCNGG 4 cut(s) 82, 272, 343, 498
BstUI CGCG 1 cut(s) 9
BstV2I GAAGAC 2 cut(s) 429, 621
BsuI GTATCC 1 cut(s) 257
BsuRI GGCC 2 cut(s) 368, 640
BtsCI GGATG 3 cut(s) 56, 109, 675
BtsIMutI CAGTG 1 cut(s) 569
Cac8I GCNNGC 1 cut(s) 234
CfoI GCGC 2 cut(s) 11, 31
Cfr13I GGNCC 4 cut(s) 121, 205, 482, 529
Csp6I GTAC 2 cut(s) 136, 541
CviAII CATG 2 cut(s) 157, 636
CviJI RGCY 6 cut(s) 150, 294, 323, 368, 640, 690
CviKI_1 RGCY 6 cut(s) 150, 294, 323, 368, 640, 690
CviQI GTAC 2 cut(s) 136, 541
DpnI GATC 3 cut(s) 496, 561, 630
DpnII GATC 3 cut(s) 494, 559, 628
EaeI YGGCCR 1 cut(s) 366
Eam1104I CTCTTC 2 cut(s) 273, 283
EarI CTCTTC 2 cut(s) 273, 283
EciI GGCGGA 1 cut(s) 34
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 4 cut(s) 121, 205, 482, 529
Eco57I CTGAAG 1 cut(s) 545
EcoO109I RGGNCCY 2 cut(s) 121, 482
EcoRII CCWGG 4 cut(s) 82, 272, 343, 498
EcoRV GATATC 1 cut(s) 445
FaeI CATG 2 cut(s) 160, 639
FaiI YATR 7 cut(s) 158, 210, 320, 393, 509, 621, 637
FaqI GGGAC 2 cut(s) 134, 468
FatI CATG 2 cut(s) 156, 635
FbaI TGATCA 2 cut(s) 559, 628
FblI GTMKAC 2 cut(s) 38, 108
Fnu4HI GCNGC 1 cut(s) 32
FokI GGATG 3 cut(s) 43, 116, 682
Fsp4HI GCNGC 1 cut(s) 32
GlaI GCGC 2 cut(s) 10, 30
GluI GCNGC 1 cut(s) 32
GsaI CCCAGC 1 cut(s) 482
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 2 cut(s) 368, 640
HhaI GCGC 2 cut(s) 11, 31
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 2 cut(s) 160, 639
Hin6I GCGC 2 cut(s) 9, 29
HinP1I GCGC 2 cut(s) 9, 29
HincII GTYRAC 2 cut(s) 39, 142
HindII GTYRAC 2 cut(s) 39, 142
HinfI GANTC 4 cut(s) 76, 222, 469, 623
HpaI GTTAAC 1 cut(s) 142
Hpy166II GTNNAC 5 cut(s) 39, 109, 136, 142, 205
Hpy188I TCNGA 4 cut(s) 64, 221, 525, 628
Hpy188III TCNNGA 3 cut(s) 226, 350, 448
Hpy8I GTNNAC 5 cut(s) 39, 109, 136, 142, 205
Hpy99I CGWCG 3 cut(s) 40, 68, 71
HpyAV CCTTC 2 cut(s) 164, 520
HpyCH4III ACNGT 2 cut(s) 454, 581
HpyCH4IV ACGT 2 cut(s) 66, 138
HpyCH4V TGCA 2 cut(s) 401, 588
HpyF10VI GCNNNNNNNGC 1 cut(s) 554
HpySE526I ACGT 2 cut(s) 66, 138
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 2 cut(s) 160, 639
HspAI GCGC 2 cut(s) 9, 29
KflI GGGWCCC 1 cut(s) 482
Ksp22I TGATCA 2 cut(s) 559, 628
KspAI GTTAAC 1 cut(s) 142
Kzo9I GATC 3 cut(s) 494, 559, 628
LweI GCATC 1 cut(s) 219
MaeII ACGT 2 cut(s) 66, 138
MaeIII GTNAC 1 cut(s) 52
MalI GATC 3 cut(s) 496, 561, 630
MboI GATC 3 cut(s) 494, 559, 628
MboII GAAGA 6 cut(s) 290, 300, 343, 395, 434, 626
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 6 cut(s) 174, 326, 377, 461, 648, 655
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 1 cut(s) 70
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 8 cut(s) 15, 52, 58, 70, 86, 161, 284, 620
Mox20I TGGCCA 1 cut(s) 368
MscI TGGCCA 1 cut(s) 368
MseI TTAA 3 cut(s) 141, 512, 551
MslI CAYNNNNRTG 1 cut(s) 213
Msp20I TGGCCA 1 cut(s) 368
MspR9I CCNGG 4 cut(s) 84, 274, 345, 500
MvaI CCWGG 4 cut(s) 84, 274, 345, 500
MvnI CGCG 1 cut(s) 9
MwoI GCNNNNNNNGC 1 cut(s) 554
NdeII GATC 3 cut(s) 494, 559, 628
NlaIII CATG 2 cut(s) 160, 639
NlaIV GGNNCC 3 cut(s) 122, 483, 484
NmuCI GTSAC 1 cut(s) 52
NspV TTCGAA 1 cut(s) 17
PfeI GAWTC 3 cut(s) 222, 469, 623
PkrI GCNGC 1 cut(s) 33
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PpuMI RGGWCCY 2 cut(s) 121, 482
Psp5II RGGWCCY 2 cut(s) 121, 482
Psp6I CCWGG 4 cut(s) 82, 272, 343, 498
PspFI CCCAGC 1 cut(s) 478
PspGI CCWGG 4 cut(s) 82, 272, 343, 498
PspN4I GGNNCC 3 cut(s) 122, 483, 484
PspPI GGNCC 4 cut(s) 121, 205, 482, 529
PspPPI RGGWCCY 2 cut(s) 121, 482
RsaI GTAC 2 cut(s) 137, 542
RsaNI GTAC 2 cut(s) 136, 541
RseI CAYNNNNRTG 1 cut(s) 213
SalI GTCGAC 1 cut(s) 37
SaqAI TTAA 3 cut(s) 141, 512, 551
SatI GCNGC 1 cut(s) 32
Sau3AI GATC 3 cut(s) 494, 559, 628
Sau96I GGNCC 4 cut(s) 121, 205, 482, 529
SchI GAGTC 1 cut(s) 70
ScrFI CCNGG 4 cut(s) 84, 274, 345, 500
SfaNI GCATC 1 cut(s) 219
SfuI TTCGAA 1 cut(s) 17
SinI GGWCC 4 cut(s) 121, 205, 482, 529
SmiMI CAYNNNNRTG 1 cut(s) 213
Sse9I AATT 6 cut(s) 174, 326, 377, 461, 648, 655
SsiI CCGC 3 cut(s) 32, 45, 251
StyD4I CCNGG 4 cut(s) 82, 272, 343, 498
TaaI ACNGT 2 cut(s) 454, 581
TaiI ACGT 2 cut(s) 69, 141
TaqI TCGA 6 cut(s) 17, 38, 74, 153, 447, 612
TasI AATT 6 cut(s) 174, 326, 377, 461, 648, 655
TatI WGTACW 1 cut(s) 540
TauI GCSGC 1 cut(s) 34
TfiI GAWTC 3 cut(s) 222, 469, 623
Tru1I TTAA 3 cut(s) 141, 512, 551
Tru9I TTAA 3 cut(s) 141, 512, 551
TscAI CASTG 1 cut(s) 569
TseFI GTSAC 1 cut(s) 52
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 2 cut(s) 260, 686
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 1 cut(s) 569
VpaK11BI GGWCC 4 cut(s) 121, 205, 482, 529
XapI RAATTY 1 cut(s) 377
XmiI GTMKAC 2 cut(s) 38, 108
ZraI GACGTC 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.