RLG00000015751

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016719
Physical Location & Seq
Reverse (-)
1182525 .. 1185545
3021 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015751

Sequence Viewer

Length: 693 bp
ATGCCTCGCGCCACTTCGAAAAGAAAAGCGCCACCGTCGACCTCCGCCTCCGTCACCTCCTCCGACGTCGTTTCCACTCGTCCCGGTAAAAAAGGCAAGAAGGAGGTAGACAAGATAGGCAGCCTCTTTGATGTGTACGCAAACAAGACCTTGGACATGATTGACCCAGAAGGAATTGTTTCTCTTTGTTCGCATTTGGGAGTGGACCATACTGATGTCAGAATCCTGATGCTTGCTTGGAAAATGAAAGCAGAAAAGCAAGGCTATTTTTCGAGGGAAGAGTGGCGAAGAGGCTTGAAAGATTTGAATGCTGACACCATTGCTAAATTGAAGAAAGCACTCCCAGGTCTGGAAAAAGAGTTGATGGCCACACCCAAATTTGAAGATTTCTATGCTTTTGCATTTCAATACTGCTTGACAGAAGACAGGCAAAAGAGTGTTGATATCGAGACCGTTTGTGAATTACTGAGTCTCGTGCTTGGTCCCAAATACCGTTCCCAGGTTGATGTATTAATAAAGTATCTGAAGGTCCAAAGTGAGTACAGGGCATTAAATGCGGATCAGTGGAGACATTTTTACCGTTTTTGCAAGGAGATAAGTTTCCCAGATCTCGAAGACTATGATTCTGATCAAGCATGGCCTGTGATAATTGATAATCTTGTTGAATGGATGAAAGAGAACAAGCAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

231

Amino Acids

26.49

Weight (kDa)

7.6

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 109 - 223 3.2e-27 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016719)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01430 FvH4_1g01430
malus_domestica MD15G1157200.v1.1
prunus_persica Prupe.7G258300_v2.0.a1 Prupe.7G258300_v2.0.a1
pyrus_communis pycom15g14100
rosa_chinensis RchiOBHm_Chr2g0086341
rosa_laevigata RLG00000015751
rosa_multiflora Rmu_sc0003572.1_g000003 Rmu_sc0004510.1_g000020
rosa_roxburghii Rroxscaffold_2G00154680
rosa_rugosa Rorug01G0466700
rosa_samantha Rh2AG016900
rosa_wichuraiana Rw2G001430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 69
AccI GTMKAC 2 cut(s) 38, 108
AccII CGCG 1 cut(s) 9
AciI CCGC 2 cut(s) 45, 557
AclWI GGATC 1 cut(s) 567
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 1 cut(s) 377
AcuI CTGAAG 1 cut(s) 545
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 2 cut(s) 137, 542
AfiI CCNNNNNNNGG 2 cut(s) 349, 499
AgsI TTSAA 6 cut(s) 298, 307, 331, 383, 407, 665
AjnI CCWGG 2 cut(s) 343, 498
AluBI AGCT 1 cut(s) 690
AluI AGCT 1 cut(s) 690
Alw26I GTCTC 3 cut(s) 443, 476, 562
AlwI GGATC 1 cut(s) 567
AoxI GGCC 2 cut(s) 366, 638
ApeKI GCWGC 1 cut(s) 120
ApoI RAATTY 1 cut(s) 377
AseI ATTAAT 1 cut(s) 512
Asp700I GAANNNNTTC 1 cut(s) 178
AspLEI GCGC 2 cut(s) 11, 31
AspS9I GGNCC 3 cut(s) 205, 482, 529
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 46
AsuII TTCGAA 1 cut(s) 17
AvaII GGWCC 3 cut(s) 205, 482, 529
BalI TGGCCA 1 cut(s) 368
BauI CACGAG 1 cut(s) 473
BbsI GAAGAC 2 cut(s) 429, 621
BbvI GCAGC 1 cut(s) 132
BccI CCATC 1 cut(s) 358
BciT130I CCWGG 2 cut(s) 345, 500
BclI TGATCA 1 cut(s) 628
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 3 cut(s) 443, 476, 562
BfaI CTAG 1 cut(s) 691
BfoI RGCGCY 1 cut(s) 32
BglII AGATCT 1 cut(s) 607
BisI GCNGC 1 cut(s) 121
BlsI GCNGC 1 cut(s) 122
Bme1390I CCNGG 3 cut(s) 84, 345, 500
Bme18I GGWCC 3 cut(s) 205, 482, 529
BmgT120I GGNCC 3 cut(s) 205, 482, 529
BmiI GGNNCC 1 cut(s) 484
BmrFI CCNGG 3 cut(s) 84, 345, 500
BmsI GCATC 1 cut(s) 219
BpiI GAAGAC 2 cut(s) 429, 621
Bpu14I TTCGAA 1 cut(s) 17
BpuMI CCSGG 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 627
BsaHI GRCGYC 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 3 cut(s) 150, 343, 498
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 499
Bse3DI GCAATG 1 cut(s) 318
Bse8I GATNNNNATC 1 cut(s) 627
BseBI CCWGG 2 cut(s) 345, 500
BseDI CCNNGG 3 cut(s) 150, 343, 498
BseGI GGATG 1 cut(s) 675
BseJI GATNNNNATC 1 cut(s) 627
BseLI CCNNNNNNNGG 2 cut(s) 349, 499
BseMI GCAATG 1 cut(s) 318
BseMII CTCAG 1 cut(s) 458
BseRI GAGGAG 1 cut(s) 49
BseXI GCAGC 1 cut(s) 132
Bsh1236I CGCG 1 cut(s) 9
BshFI GGCC 2 cut(s) 368, 640
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 2 cut(s) 66, 468
BslI CCNNNNNNNGG 2 cut(s) 349, 499
BsmAI GTCTC 3 cut(s) 443, 476, 562
BsmFI GGGAC 2 cut(s) 66, 468
BsmI GAATGC 1 cut(s) 313
BsnI GGCC 2 cut(s) 368, 640
Bso31I GGTCTC 1 cut(s) 443
Bsp119I TTCGAA 1 cut(s) 17
Bsp143I GATC 3 cut(s) 559, 607, 628
BspACI CCGC 2 cut(s) 45, 557
BspANI GGCC 2 cut(s) 368, 640
BspCNI CTCAG 1 cut(s) 459
BspFNI CGCG 1 cut(s) 9
BspLI GGNNCC 1 cut(s) 484
BspPI GGATC 1 cut(s) 567
BspT104I TTCGAA 1 cut(s) 17
BspTNI GGTCTC 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 318
BssECI CCNNGG 3 cut(s) 150, 343, 498
BssMI GATC 3 cut(s) 559, 607, 628
BssNI GRCGYC 1 cut(s) 66
BssSI CACGAG 1 cut(s) 473
BssT1I CCWWGG 1 cut(s) 150
Bst2BI CACGAG 1 cut(s) 473
Bst2UI CCWGG 2 cut(s) 345, 500
Bst4CI ACNGT 4 cut(s) 36, 454, 494, 581
Bst6I CTCTTC 2 cut(s) 273, 283
BstACI GRCGYC 1 cut(s) 66
BstAPI GCANNNNNTGC 1 cut(s) 554
BstBI TTCGAA 1 cut(s) 17
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 1 cut(s) 467
BstF5I GGATG 1 cut(s) 675
BstFNI CGCG 1 cut(s) 9
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 2 cut(s) 11, 31
BstKTI GATC 3 cut(s) 562, 610, 631
BstMAI GTCTC 3 cut(s) 443, 476, 562
BstMBI GATC 3 cut(s) 559, 607, 628
BstMWI GCNNNNNNNGC 1 cut(s) 554
BstNI CCWGG 2 cut(s) 345, 500
BstSCI CCNGG 3 cut(s) 82, 343, 498
BstUI CGCG 1 cut(s) 9
BstV1I GCAGC 1 cut(s) 132
BstV2I GAAGAC 2 cut(s) 429, 621
BstX2I RGATCY 1 cut(s) 607
BstYI RGATCY 1 cut(s) 607
BsuRI GGCC 2 cut(s) 368, 640
BtsCI GGATG 1 cut(s) 675
BtsIMutI CAGTG 1 cut(s) 569
Cac8I GCNNGC 1 cut(s) 234
CfoI GCGC 2 cut(s) 11, 31
Cfr13I GGNCC 3 cut(s) 205, 482, 529
Csp6I GTAC 2 cut(s) 136, 541
CviAII CATG 2 cut(s) 157, 636
CviJI RGCY 6 cut(s) 123, 264, 294, 368, 640, 690
CviKI_1 RGCY 6 cut(s) 123, 264, 294, 368, 640, 690
CviQI GTAC 2 cut(s) 136, 541
DdeI CTNAG 1 cut(s) 467
DpnI GATC 3 cut(s) 561, 609, 630
DpnII GATC 3 cut(s) 559, 607, 628
EaeI YGGCCR 1 cut(s) 366
Eam1104I CTCTTC 2 cut(s) 273, 283
EarI CTCTTC 2 cut(s) 273, 283
EciI GGCGGA 1 cut(s) 34
Eco130I CCWWGG 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 443
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 3 cut(s) 205, 482, 529
Eco57I CTGAAG 1 cut(s) 545
EcoRII CCWGG 2 cut(s) 343, 498
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 2 cut(s) 160, 639
FaiI YATR 5 cut(s) 158, 210, 393, 621, 637
FaqI GGGAC 2 cut(s) 66, 468
FatI CATG 2 cut(s) 156, 635
FbaI TGATCA 1 cut(s) 628
FblI GTMKAC 2 cut(s) 38, 108
Fnu4HI GCNGC 1 cut(s) 121
FokI GGATG 1 cut(s) 682
Fsp4HI GCNGC 1 cut(s) 121
FspBI CTAG 1 cut(s) 691
GlaI GCGC 2 cut(s) 10, 30
GluI GCNGC 1 cut(s) 121
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 2 cut(s) 368, 640
HapII CCGG 1 cut(s) 84
HhaI GCGC 2 cut(s) 11, 31
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 2 cut(s) 160, 639
Hin6I GCGC 2 cut(s) 9, 29
HinP1I GCGC 2 cut(s) 9, 29
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HinfI GANTC 3 cut(s) 222, 469, 623
HpaII CCGG 1 cut(s) 84
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 4 cut(s) 39, 109, 136, 205
Hpy188I TCNGA 4 cut(s) 64, 221, 525, 628
Hpy188III TCNNGA 4 cut(s) 226, 350, 448, 611
Hpy8I GTNNAC 4 cut(s) 39, 109, 136, 205
Hpy99I CGWCG 3 cut(s) 40, 68, 71
HpyAV CCTTC 3 cut(s) 94, 164, 520
HpyCH4III ACNGT 4 cut(s) 36, 454, 494, 581
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 2 cut(s) 401, 588
HpyF10VI GCNNNNNNNGC 1 cut(s) 554
HpyF3I CTNAG 1 cut(s) 467
HpySE526I ACGT 1 cut(s) 66
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 2 cut(s) 160, 639
HspAI GCGC 2 cut(s) 9, 29
Ksp22I TGATCA 1 cut(s) 628
Kzo9I GATC 3 cut(s) 559, 607, 628
Lsp1109I GCAGC 1 cut(s) 132
LweI GCATC 1 cut(s) 219
MaeI CTAG 1 cut(s) 691
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 1 cut(s) 52
MalI GATC 3 cut(s) 561, 609, 630
MboI GATC 3 cut(s) 559, 607, 628
MboII GAAGA 6 cut(s) 290, 300, 343, 395, 434, 626
MflI RGATCY 1 cut(s) 607
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 5 cut(s) 174, 326, 377, 461, 648
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 1 cut(s) 478
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 9 cut(s) 15, 52, 58, 67, 70, 97, 134, 267, 284
Mox20I TGGCCA 1 cut(s) 368
MroXI GAANNNNTTC 1 cut(s) 178
MscI TGGCCA 1 cut(s) 368
MseI TTAA 2 cut(s) 512, 551
MslI CAYNNNNRTG 1 cut(s) 213
Msp20I TGGCCA 1 cut(s) 368
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 3 cut(s) 84, 345, 500
Mva1269I GAATGC 1 cut(s) 313
MvaI CCWGG 2 cut(s) 345, 500
MvnI CGCG 1 cut(s) 9
MwoI GCNNNNNNNGC 1 cut(s) 554
NciI CCSGG 1 cut(s) 84
NdeII GATC 3 cut(s) 559, 607, 628
NlaIII CATG 2 cut(s) 160, 639
NlaIV GGNNCC 1 cut(s) 484
NmuCI GTSAC 1 cut(s) 52
NspV TTCGAA 1 cut(s) 17
PctI GAATGC 1 cut(s) 313
PdmI GAANNNNTTC 1 cut(s) 178
PfeI GAWTC 2 cut(s) 222, 623
PkrI GCNGC 1 cut(s) 122
PleI GAGTC 1 cut(s) 477
PpsI GAGTC 1 cut(s) 477
PshBI ATTAAT 1 cut(s) 512
Psp6I CCWGG 2 cut(s) 343, 498
PspGI CCWGG 2 cut(s) 343, 498
PspN4I GGNNCC 1 cut(s) 484
PspPI GGNCC 3 cut(s) 205, 482, 529
PsuI RGATCY 1 cut(s) 607
RsaI GTAC 2 cut(s) 137, 542
RsaNI GTAC 2 cut(s) 136, 541
RseI CAYNNNNRTG 1 cut(s) 213
SalI GTCGAC 1 cut(s) 37
SaqAI TTAA 2 cut(s) 512, 551
SatI GCNGC 1 cut(s) 121
Sau3AI GATC 3 cut(s) 559, 607, 628
Sau96I GGNCC 3 cut(s) 205, 482, 529
SchI GAGTC 1 cut(s) 478
ScrFI CCNGG 3 cut(s) 84, 345, 500
SetI ASST 9 cut(s) 44, 59, 69, 108, 152, 349, 504, 531, 692
SfaNI GCATC 1 cut(s) 219
SfuI TTCGAA 1 cut(s) 17
SinI GGWCC 3 cut(s) 205, 482, 529
SmiMI CAYNNNNRTG 1 cut(s) 213
Sse9I AATT 5 cut(s) 174, 326, 377, 461, 648
SsiI CCGC 2 cut(s) 45, 557
SspMI CTAG 1 cut(s) 691
StyD4I CCNGG 3 cut(s) 82, 343, 498
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 4 cut(s) 36, 454, 494, 581
TaiI ACGT 1 cut(s) 69
TaqI TCGA 5 cut(s) 17, 38, 272, 447, 612
TasI AATT 5 cut(s) 174, 326, 377, 461, 648
TatI WGTACW 1 cut(s) 540
TfiI GAWTC 2 cut(s) 222, 623
Tru1I TTAA 2 cut(s) 512, 551
Tru9I TTAA 2 cut(s) 512, 551
TscAI CASTG 1 cut(s) 569
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 1 cut(s) 120
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 2 cut(s) 260, 686
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 1 cut(s) 569
VpaK11BI GGWCC 3 cut(s) 205, 482, 529
VspI ATTAAT 1 cut(s) 512
XapI RAATTY 1 cut(s) 377
XmiI GTMKAC 2 cut(s) 38, 108
XmnI GAANNNNTTC 1 cut(s) 178
XspI CTAG 1 cut(s) 691
ZraI GACGTC 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.