Prupe.7G258300_v2.0.a1

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0016719
Physical Location & Seq
Forward (+)
21562094 .. 21566548
4455 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G258300.2

Sequence Viewer

Length: 690 bp
ATGCCTCGCGCTTCTAAAAGAAAAGCAGCTCCATCAACTTCGACTTCCGTCACATCCTGCGACGCCGTTCCACCGCGTGCCGGTAAATGTAAATCAAGAAAGGTGGTAGACCGGATAGGAAGCCTTTTTGAATTGTATGCAAACCAGTCGTTAAACATGATTGACCCAGAAGGAATTGAGGCTCTTTGTTCGGATTTGGGTGTGGACCATACTAATGTTAGAATACTGATGCTTGCTTGGAAAATGAAAGCTGAAAAGCAGGGCTACTTTTCCAGGGACGAGTGGCAAAGAGGCTTGAAAGAGCTACATGTCGACACTATTTCTAAATTAAAGAAAGCACTCCCGGGTTTGGAGAAAGAGTTGATGACCCCACCCAAATTTGAAGATTTTTATGCATATGCATTTCGATACTGCTTGACAGAAGAAAGGCAAAAGAGTGTAGACATTGAGACTGTATGTGAATTACTGAATCTCGTATTGGGTCCCCTATACCGTCTCCAGGTTGATTTATTAATTAAGTATTTGAAGGTCCAAAGTGAGTACAGAGCATTAAATATGGATCAGTGGTTACATTTTTACCGTTTTTGCAAGGAGATAAGTTTTCCGGACCTTCAAAACTATGATTCGGATCAAGCATGGCCTGTGATTATTGATAATTTTGTTGAATGGATGAGAGACGAGCAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

230

Amino Acids

26.65

Weight (kDa)

6.34

Isoelectric Point (pI)

40.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016719)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01430 FvH4_1g01430
malus_domestica MD15G1157200.v1.1
prunus_persica Prupe.7G258300_v2.0.a1 Prupe.7G258300_v2.0.a1
pyrus_communis pycom15g14100
rosa_chinensis RchiOBHm_Chr2g0086341
rosa_laevigata RLG00000015751
rosa_multiflora Rmu_sc0003572.1_g000003 Rmu_sc0004510.1_g000020
rosa_roxburghii Rroxscaffold_2G00154680
rosa_rugosa Rorug01G0466700
rosa_samantha Rh2AG016900
rosa_wichuraiana Rw2G001430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 108, 312, 441
AccII CGCG 2 cut(s) 9, 76
AccIII TCCGGA 1 cut(s) 604
AciI CCGC 1 cut(s) 74
AclWI GGATC 2 cut(s) 567, 636
AcsI RAATTY 1 cut(s) 377
AcyI GRCGYC 1 cut(s) 63
AdeI CACNNNGTG 1 cut(s) 77
AfaI GTAC 1 cut(s) 542
AfiI CCNNNNNNNGG 3 cut(s) 80, 349, 499
AflIII ACRYGT 1 cut(s) 307
AgsI TTSAA 6 cut(s) 131, 298, 383, 526, 614, 665
AjnI CCWGG 2 cut(s) 272, 498
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AluBI AGCT 4 cut(s) 29, 251, 304, 687
AluI AGCT 4 cut(s) 29, 251, 304, 687
Alw26I GTCTC 3 cut(s) 443, 500, 669
AlwI GGATC 2 cut(s) 567, 636
Ama87I CYCGRG 1 cut(s) 343
Aor13HI TCCGGA 1 cut(s) 604
AoxI GGCC 1 cut(s) 638
ApeKI GCWGC 1 cut(s) 26
ApoI RAATTY 1 cut(s) 377
AseI ATTAAT 1 cut(s) 512
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 4 cut(s) 205, 482, 529, 607
AsuC2I CCSGG 2 cut(s) 344, 345
AvaI CYCGRG 1 cut(s) 343
AvaII GGWCC 4 cut(s) 205, 482, 529, 607
BbvI GCAGC 1 cut(s) 38
BccI CCATC 1 cut(s) 40
BceAI ACGGC 1 cut(s) 50
BcgI CGANNNNNNTGC 2 cut(s) 129, 163
BciT130I CCWGG 2 cut(s) 274, 500
BcnI CCSGG 2 cut(s) 344, 345
BcoDI GTCTC 3 cut(s) 443, 500, 669
BisI GCNGC 1 cut(s) 27
BlsI GCNGC 1 cut(s) 28
Bme1390I CCNGG 4 cut(s) 274, 344, 345, 500
Bme18I GGWCC 4 cut(s) 205, 482, 529, 607
BmeT110I CYCGRG 1 cut(s) 343
BmgT120I GGNCC 4 cut(s) 205, 482, 529, 607
BmiI GGNNCC 2 cut(s) 483, 484
BmrFI CCNGG 4 cut(s) 274, 344, 345, 500
BmsI GCATC 1 cut(s) 219
BoxI GACNNNNGTC 1 cut(s) 47
BpmI CTGGAG 1 cut(s) 482
BpuMI CCSGG 2 cut(s) 344, 345
BsaBI GATNNNNATC 1 cut(s) 627
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 2 cut(s) 273, 343
BsaWI WCCGGW 2 cut(s) 111, 604
BsaXI ACNNNNNCTCC 2 cut(s) 344, 374
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 349, 499
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 1 cut(s) 145
Bse8I GATNNNNATC 1 cut(s) 627
BseAI TCCGGA 1 cut(s) 604
BseBI CCWGG 2 cut(s) 274, 500
BseDI CCNNGG 2 cut(s) 273, 343
BseGI GGATG 2 cut(s) 53, 675
BseJI GATNNNNATC 1 cut(s) 627
BseLI CCNNNNNNNGG 3 cut(s) 80, 349, 499
BseNI ACTGG 1 cut(s) 145
BseXI GCAGC 1 cut(s) 38
Bsh1236I CGCG 2 cut(s) 9, 76
BshFI GGCC 1 cut(s) 640
BsiHKCI CYCGRG 1 cut(s) 343
BsiSI CCGG 4 cut(s) 81, 112, 344, 605
BslFI GGGAC 2 cut(s) 290, 468
BslI CCNNNNNNNGG 3 cut(s) 80, 349, 499
BsmAI GTCTC 3 cut(s) 443, 500, 669
BsmBI CGTCTC 2 cut(s) 500, 669
BsmFI GGGAC 2 cut(s) 290, 468
BsnI GGCC 1 cut(s) 640
BsoBI CYCGRG 1 cut(s) 343
Bsp13I TCCGGA 1 cut(s) 604
Bsp143I GATC 2 cut(s) 559, 628
BspACI CCGC 1 cut(s) 74
BspANI GGCC 1 cut(s) 640
BspEI TCCGGA 1 cut(s) 604
BspFNI CGCG 2 cut(s) 9, 76
BspLI GGNNCC 2 cut(s) 483, 484
BspPI GGATC 2 cut(s) 567, 636
BsrFI RCCGGY 1 cut(s) 80
BsrI ACTGG 1 cut(s) 145
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 2 cut(s) 273, 343
BssMI GATC 2 cut(s) 559, 628
BssNI GRCGYC 1 cut(s) 63
Bst2UI CCWGG 2 cut(s) 274, 500
Bst4CI ACNGT 3 cut(s) 454, 494, 581
BstACI GRCGYC 1 cut(s) 63
BstC8I GCNNGC 2 cut(s) 78, 234
BstF5I GGATG 2 cut(s) 53, 675
BstFNI CGCG 2 cut(s) 9, 76
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 2 cut(s) 562, 631
BstMAI GTCTC 3 cut(s) 443, 500, 669
BstMBI GATC 2 cut(s) 559, 628
BstNI CCWGG 2 cut(s) 274, 500
BstNSI RCATGY 1 cut(s) 311
BstPAI GACNNNNGTC 1 cut(s) 47
BstSCI CCNGG 4 cut(s) 272, 342, 343, 498
BstUI CGCG 2 cut(s) 9, 76
BstV1I GCAGC 1 cut(s) 38
BsuRI GGCC 1 cut(s) 640
BtsCI GGATG 2 cut(s) 53, 675
BtsIMutI CAGTG 1 cut(s) 569
Cac8I GCNNGC 2 cut(s) 78, 234
CfoI GCGC 1 cut(s) 11
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 4 cut(s) 205, 482, 529, 607
Cfr9I CCCGGG 1 cut(s) 343
CseI GACGC 1 cut(s) 71
Csp6I GTAC 1 cut(s) 541
CspCI CAANNNNNGTGG 2 cut(s) 84, 119
CviAII CATG 3 cut(s) 157, 308, 636
CviJI RGCY 9 cut(s) 29, 123, 182, 251, 264, 294, 304, 640, 687
CviKI_1 RGCY 9 cut(s) 29, 123, 182, 251, 264, 294, 304, 640, 687
CviQI GTAC 1 cut(s) 541
DpnI GATC 2 cut(s) 561, 630
DpnII GATC 2 cut(s) 559, 628
DraIII CACNNNGTG 1 cut(s) 77
Eco47I GGWCC 4 cut(s) 205, 482, 529, 607
Eco88I CYCGRG 1 cut(s) 343
EcoO109I RGGNCCY 1 cut(s) 482
EcoRII CCWGG 2 cut(s) 272, 498
EcoT22I ATGCAT 2 cut(s) 397, 403
Esp3I CGTCTC 2 cut(s) 500, 669
FaeI CATG 3 cut(s) 160, 311, 639
FaqI GGGAC 2 cut(s) 290, 468
FatI CATG 3 cut(s) 156, 307, 635
FauNDI CATATG 1 cut(s) 397
FblI GTMKAC 3 cut(s) 108, 312, 441
Fnu4HI GCNGC 1 cut(s) 27
FokI GGATG 2 cut(s) 40, 682
Fsp4HI GCNGC 1 cut(s) 27
GlaI GCGC 1 cut(s) 10
GluI GCNGC 1 cut(s) 27
GsuI CTGGAG 1 cut(s) 482
HaeIII GGCC 1 cut(s) 640
HapII CCGG 4 cut(s) 81, 112, 344, 605
HgaI GACGC 1 cut(s) 71
HhaI GCGC 1 cut(s) 11
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 3 cut(s) 160, 311, 639
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HinfI GANTC 2 cut(s) 469, 623
HpaII CCGG 4 cut(s) 81, 112, 344, 605
Hpy166II GTNNAC 4 cut(s) 109, 205, 313, 442
Hpy188I TCNGA 2 cut(s) 193, 628
Hpy188III TCNNGA 2 cut(s) 96, 605
Hpy8I GTNNAC 4 cut(s) 109, 205, 313, 442
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 3 cut(s) 164, 520, 620
HpyCH4III ACNGT 3 cut(s) 454, 494, 581
HpyCH4V TGCA 4 cut(s) 140, 395, 401, 588
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 3 cut(s) 160, 311, 639
HspAI GCGC 1 cut(s) 9
KflI GGGWCCC 1 cut(s) 482
Kpn2I TCCGGA 1 cut(s) 604
Kzo9I GATC 2 cut(s) 559, 628
LmnI GCTCC 1 cut(s) 34
Lsp1109I GCAGC 1 cut(s) 38
LweI GCATC 1 cut(s) 219
MaeIII GTNAC 2 cut(s) 49, 567
MalI GATC 2 cut(s) 561, 630
MboI GATC 2 cut(s) 559, 628
MboII GAAGA 2 cut(s) 395, 434
MluCI AATT 7 cut(s) 131, 174, 326, 377, 461, 513, 655
MnlI CCTC 3 cut(s) 15, 172, 284
Mph1103I ATGCAT 2 cut(s) 397, 403
MroI TCCGGA 1 cut(s) 604
MseI TTAA 5 cut(s) 152, 329, 512, 516, 551
MslI CAYNNNNRTG 1 cut(s) 213
MspI CCGG 4 cut(s) 81, 112, 344, 605
MspR9I CCNGG 4 cut(s) 274, 344, 345, 500
MvaI CCWGG 2 cut(s) 274, 500
MvnI CGCG 2 cut(s) 9, 76
NciI CCSGG 2 cut(s) 344, 345
NdeI CATATG 1 cut(s) 397
NdeII GATC 2 cut(s) 559, 628
NlaIII CATG 3 cut(s) 160, 311, 639
NlaIV GGNNCC 2 cut(s) 483, 484
NmuCI GTSAC 1 cut(s) 49
NsiI ATGCAT 2 cut(s) 397, 403
NspI RCATGY 1 cut(s) 311
PacI TTAATTAA 1 cut(s) 516
PciI ACATGT 1 cut(s) 307
PfeI GAWTC 2 cut(s) 469, 623
PkrI GCNGC 1 cut(s) 28
PpuMI RGGWCCY 1 cut(s) 482
PscI ACATGT 1 cut(s) 307
PshAI GACNNNNGTC 1 cut(s) 47
PshBI ATTAAT 1 cut(s) 512
Psp5II RGGWCCY 1 cut(s) 482
Psp6I CCWGG 2 cut(s) 272, 498
PspGI CCWGG 2 cut(s) 272, 498
PspN4I GGNNCC 2 cut(s) 483, 484
PspPI GGNCC 4 cut(s) 205, 482, 529, 607
PspPPI RGGWCCY 1 cut(s) 482
RsaI GTAC 1 cut(s) 542
RsaNI GTAC 1 cut(s) 541
RseI CAYNNNNRTG 1 cut(s) 213
SalI GTCGAC 1 cut(s) 311
SaqAI TTAA 5 cut(s) 152, 329, 512, 516, 551
SatI GCNGC 1 cut(s) 27
Sau3AI GATC 2 cut(s) 559, 628
Sau96I GGNCC 4 cut(s) 205, 482, 529, 607
ScrFI CCNGG 4 cut(s) 274, 344, 345, 500
SetI ASST 8 cut(s) 31, 105, 253, 306, 504, 531, 612, 689
SfaNI GCATC 1 cut(s) 219
SinI GGWCC 4 cut(s) 205, 482, 529, 607
SmaI CCCGGG 1 cut(s) 345
SmiMI CAYNNNNRTG 1 cut(s) 213
Sse9I AATT 7 cut(s) 131, 174, 326, 377, 461, 513, 655
SsiI CCGC 1 cut(s) 74
StyD4I CCNGG 4 cut(s) 272, 342, 343, 498
TaaI ACNGT 3 cut(s) 454, 494, 581
TaqI TCGA 3 cut(s) 41, 312, 406
TasI AATT 7 cut(s) 131, 174, 326, 377, 461, 513, 655
TatI WGTACW 1 cut(s) 540
TfiI GAWTC 2 cut(s) 469, 623
Tru1I TTAA 5 cut(s) 152, 329, 512, 516, 551
Tru9I TTAA 5 cut(s) 152, 329, 512, 516, 551
TscAI CASTG 1 cut(s) 569
TseFI GTSAC 1 cut(s) 49
TseI GCWGC 1 cut(s) 26
Tsp45I GTSAC 1 cut(s) 49
TspDTI ATGAA 1 cut(s) 260
TspGWI ACGGA 1 cut(s) 37
TspMI CCCGGG 1 cut(s) 343
TspRI CASTG 1 cut(s) 569
VpaK11BI GGWCC 4 cut(s) 205, 482, 529, 607
VspI ATTAAT 1 cut(s) 512
XapI RAATTY 1 cut(s) 377
XceI RCATGY 1 cut(s) 311
XmaI CCCGGG 1 cut(s) 343
XmiI GTMKAC 3 cut(s) 108, 312, 441
Zsp2I ATGCAT 2 cut(s) 397, 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.