MD15G1157200.v1.1

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016719
Physical Location & Seq
Reverse (-)
11666957 .. 11670645
3689 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1157200.v1.1.491

Sequence Viewer

Length: 696 bp
ATGCCTCGCGCTTCTAAGAGAAAAGCACCTCCACCAACTTCGCCTTCCCTCGCGTCCTGCGACGTCGTTTCGAAGCGCGCCGCAACAGGTAAAGGCAGACCAAGAAAGGATGTAGACAAAATAGATAGCCTCTTTGAATCGTATGCAAACCAGTCGTTAAACATGATTGACCCAACAGGAATCGAGGCTCTTTGTTCGGACTTGAAAGTGGACCATACCGATGTTAGAATACTGATGCTTGCTTGGAAAATGAGAGCTGCAAAGCAGGGCTACTTTTCAAAGGATGAGTGGCAAAGAGGCTTGAAAGATCTACATGCTGACACTATCCCTAAATTAAAGAAAGCACTACCGGGATTGGAGAAAGAGTTGACAACAGCGTCCAAATTTGAAGATTTCTATGCTTTTGCATTTCAATACTGCTTGACAGAGGAAAGGCAAAAGAGTGTAGACATTGAGACTGTGTGTGAATTACTGAATCTCGTACTGGGTCCTCGATACCAGCTCCAGGTTGATTTATTGATCAAGTATTTGAAGGTCCAAACTGAGTACAGGGCACTAAATATGGATCAGTGGATGCATTTTTACCGTTTTTGCAATGAGATAAGTTTTCCAGACCTTAAAAATTATGATTCTGATCAAGCATGGCCGGTGATTTTTGATAACTTTGTTGAATGGGTGAGAGACAAGCAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

232

Amino Acids

26.77

Weight (kDa)

7.58

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 111 - 225 6e-29 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016719)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01430 FvH4_1g01430
malus_domestica MD15G1157200.v1.1
prunus_persica Prupe.7G258300_v2.0.a1 Prupe.7G258300_v2.0.a1
pyrus_communis pycom15g14100
rosa_chinensis RchiOBHm_Chr2g0086341
rosa_laevigata RLG00000015751
rosa_multiflora Rmu_sc0003572.1_g000003 Rmu_sc0004510.1_g000020
rosa_roxburghii Rroxscaffold_2G00154680
rosa_rugosa Rorug01G0466700
rosa_samantha Rh2AG016900
rosa_wichuraiana Rw2G001430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 376
AatII GACGTC 1 cut(s) 66
AccI GTMKAC 2 cut(s) 114, 447
AccII CGCG 3 cut(s) 9, 53, 78
AciI CCGC 1 cut(s) 81
AclWI GGATC 1 cut(s) 573
AcoI YGGCCR 1 cut(s) 644
AcsI RAATTY 1 cut(s) 383
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 2 cut(s) 483, 548
AfiI CCNNNNNNNGG 1 cut(s) 505
AgsI TTSAA 8 cut(s) 137, 205, 279, 304, 389, 413, 532, 671
AjnI CCWGG 1 cut(s) 504
AjuI GAANNNNNNNTTGG 2 cut(s) 28, 60
AluBI AGCT 3 cut(s) 257, 502, 693
AluI AGCT 3 cut(s) 257, 502, 693
Alw26I GTCTC 2 cut(s) 449, 675
AlwI GGATC 1 cut(s) 573
AoxI GGCC 1 cut(s) 644
ApeKI GCWGC 1 cut(s) 257
ApoI RAATTY 1 cut(s) 383
AspLEI GCGC 3 cut(s) 11, 78, 80
AspS9I GGNCC 3 cut(s) 211, 488, 535
AsuC2I CCSGG 1 cut(s) 351
AsuHPI GGTGA 2 cut(s) 661, 688
AsuII TTCGAA 1 cut(s) 71
AvaII GGWCC 3 cut(s) 211, 488, 535
BaeGI GKGCMC 1 cut(s) 556
BbvI GCAGC 1 cut(s) 244
BcgI CGANNNNNNTGC 2 cut(s) 135, 169
BciT130I CCWGG 1 cut(s) 506
BclI TGATCA 2 cut(s) 519, 634
BcnI CCSGG 1 cut(s) 351
BcoDI GTCTC 2 cut(s) 449, 675
BglII AGATCT 1 cut(s) 307
BisI GCNGC 2 cut(s) 81, 258
BlsI GCNGC 2 cut(s) 82, 259
Bme1390I CCNGG 2 cut(s) 351, 506
Bme18I GGWCC 3 cut(s) 211, 488, 535
BmgT120I GGNCC 3 cut(s) 211, 488, 535
BmiI GGNNCC 1 cut(s) 489
BmrFI CCNGG 2 cut(s) 351, 506
BmrI ACTGGG 1 cut(s) 494
BmsI GCATC 2 cut(s) 225, 564
BmuI ACTGGG 1 cut(s) 494
BpmI CTGGAG 1 cut(s) 488
Bpu14I TTCGAA 1 cut(s) 71
BpuMI CCSGG 1 cut(s) 351
BsaBI GATNNNNATC 1 cut(s) 633
BsaHI GRCGYC 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 350, 380
Bsc4I CCNNNNNNNGG 1 cut(s) 505
Bse118I RCCGGY 1 cut(s) 646
Bse1I ACTGG 2 cut(s) 151, 489
Bse3DI GCAATG 1 cut(s) 601
Bse8I GATNNNNATC 1 cut(s) 633
BseBI CCWGG 1 cut(s) 506
BseGI GGATG 3 cut(s) 115, 289, 579
BseJI GATNNNNATC 1 cut(s) 633
BseLI CCNNNNNNNGG 1 cut(s) 505
BseMI GCAATG 1 cut(s) 601
BseMII CTCAG 1 cut(s) 534
BseNI ACTGG 2 cut(s) 151, 489
BsePI GCGCGC 1 cut(s) 76
BseSI GKGCMC 1 cut(s) 556
BseXI GCAGC 1 cut(s) 244
Bsh1236I CGCG 3 cut(s) 9, 53, 78
BshFI GGCC 1 cut(s) 646
BsiSI CCGG 2 cut(s) 350, 647
BslI CCNNNNNNNGG 1 cut(s) 505
BsmAI GTCTC 2 cut(s) 449, 675
BsnI GGCC 1 cut(s) 646
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 556
Bsp143I GATC 4 cut(s) 307, 519, 565, 634
BspACI CCGC 1 cut(s) 81
BspANI GGCC 1 cut(s) 646
BspCNI CTCAG 1 cut(s) 535
BspFNI CGCG 3 cut(s) 9, 53, 78
BspLI GGNNCC 1 cut(s) 489
BspPI GGATC 1 cut(s) 573
BspT104I TTCGAA 1 cut(s) 71
BsrDI GCAATG 1 cut(s) 601
BsrFI RCCGGY 1 cut(s) 646
BsrI ACTGG 2 cut(s) 151, 489
BssAI RCCGGY 1 cut(s) 646
BssHII GCGCGC 1 cut(s) 76
BssMI GATC 4 cut(s) 307, 519, 565, 634
BssNI GRCGYC 1 cut(s) 63
Bst2UI CCWGG 1 cut(s) 506
Bst4CI ACNGT 2 cut(s) 460, 587
BstACI GRCGYC 1 cut(s) 63
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 2 cut(s) 78, 240
BstDEI CTNAG 2 cut(s) 15, 543
BstF5I GGATG 3 cut(s) 115, 289, 579
BstFNI CGCG 3 cut(s) 9, 53, 78
BstHHI GCGC 3 cut(s) 11, 78, 80
BstKTI GATC 4 cut(s) 310, 522, 568, 637
BstMAI GTCTC 2 cut(s) 449, 675
BstMBI GATC 4 cut(s) 307, 519, 565, 634
BstNI CCWGG 1 cut(s) 506
BstNSI RCATGY 1 cut(s) 317
BstSCI CCNGG 2 cut(s) 349, 504
BstSLI GKGCMC 1 cut(s) 556
BstUI CGCG 3 cut(s) 9, 53, 78
BstV1I GCAGC 1 cut(s) 244
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BsuRI GGCC 1 cut(s) 646
BtsCI GGATG 3 cut(s) 115, 289, 579
BtsIMutI CAGTG 1 cut(s) 575
Cac8I GCNNGC 2 cut(s) 78, 240
CfoI GCGC 3 cut(s) 11, 78, 80
Cfr10I RCCGGY 1 cut(s) 646
Cfr13I GGNCC 3 cut(s) 211, 488, 535
CseI GACGC 2 cut(s) 42, 366
Csp6I GTAC 2 cut(s) 482, 547
CviAII CATG 3 cut(s) 163, 314, 642
CviJI RGCY 8 cut(s) 129, 188, 257, 270, 300, 502, 646, 693
CviKI_1 RGCY 8 cut(s) 129, 188, 257, 270, 300, 502, 646, 693
CviQI GTAC 2 cut(s) 482, 547
DdeI CTNAG 2 cut(s) 15, 543
DpnI GATC 4 cut(s) 309, 521, 567, 636
DpnII GATC 4 cut(s) 307, 519, 565, 634
DrdI GACNNNNNNGTC 1 cut(s) 376
DseDI GACNNNNNNGTC 1 cut(s) 376
EaeI YGGCCR 1 cut(s) 644
Eco47I GGWCC 3 cut(s) 211, 488, 535
EcoO109I RGGNCCY 1 cut(s) 488
EcoRII CCWGG 1 cut(s) 504
EcoT22I ATGCAT 1 cut(s) 579
FaeI CATG 3 cut(s) 166, 317, 645
FaiI YATR 8 cut(s) 144, 164, 216, 315, 399, 563, 627, 643
FatI CATG 3 cut(s) 162, 313, 641
FbaI TGATCA 2 cut(s) 519, 634
FblI GTMKAC 2 cut(s) 114, 447
Fnu4HI GCNGC 2 cut(s) 81, 258
FokI GGATG 3 cut(s) 122, 296, 586
Fsp4HI GCNGC 2 cut(s) 81, 258
GlaI GCGC 3 cut(s) 10, 77, 79
GluI GCNGC 2 cut(s) 81, 258
GsuI CTGGAG 1 cut(s) 488
HaeIII GGCC 1 cut(s) 646
HapII CCGG 2 cut(s) 350, 647
HgaI GACGC 2 cut(s) 42, 366
HhaI GCGC 3 cut(s) 11, 78, 80
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 3 cut(s) 166, 317, 645
Hin6I GCGC 3 cut(s) 9, 76, 78
HinP1I GCGC 3 cut(s) 9, 76, 78
HincII GTYRAC 1 cut(s) 369
HindII GTYRAC 1 cut(s) 369
HinfI GANTC 4 cut(s) 137, 180, 475, 629
HpaII CCGG 2 cut(s) 350, 647
HphI GGTGA 2 cut(s) 661, 688
Hpy166II GTNNAC 4 cut(s) 115, 211, 369, 448
Hpy188I TCNGA 2 cut(s) 199, 634
Hpy188III TCNNGA 1 cut(s) 611
Hpy8I GTNNAC 4 cut(s) 115, 211, 369, 448
Hpy99I CGWCG 2 cut(s) 65, 68
HpyAV CCTTC 2 cut(s) 54, 526
HpyCH4III ACNGT 2 cut(s) 460, 587
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 5 cut(s) 146, 260, 407, 577, 594
HpyF3I CTNAG 2 cut(s) 15, 543
HpySE526I ACGT 1 cut(s) 63
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 3 cut(s) 166, 317, 645
HspAI GCGC 3 cut(s) 9, 76, 78
Ksp22I TGATCA 2 cut(s) 519, 634
Kzo9I GATC 4 cut(s) 307, 519, 565, 634
LmnI GCTCC 1 cut(s) 507
Lsp1109I GCAGC 1 cut(s) 244
LweI GCATC 2 cut(s) 225, 564
MaeII ACGT 1 cut(s) 63
MalI GATC 4 cut(s) 309, 521, 567, 636
MboI GATC 4 cut(s) 307, 519, 565, 634
MboII GAAGA 1 cut(s) 401
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 1 cut(s) 556
MluCI AATT 4 cut(s) 332, 383, 467, 622
MnlI CCTC 8 cut(s) 15, 39, 59, 140, 178, 290, 421, 501
Mph1103I ATGCAT 1 cut(s) 579
MseI TTAA 3 cut(s) 158, 335, 618
MslI CAYNNNNRTG 1 cut(s) 219
MspI CCGG 2 cut(s) 350, 647
MspR9I CCNGG 2 cut(s) 351, 506
MvaI CCWGG 1 cut(s) 506
MvnI CGCG 3 cut(s) 9, 53, 78
NciI CCSGG 1 cut(s) 351
NdeII GATC 4 cut(s) 307, 519, 565, 634
NlaIII CATG 3 cut(s) 166, 317, 645
NlaIV GGNNCC 1 cut(s) 489
NsiI ATGCAT 1 cut(s) 579
NspI RCATGY 1 cut(s) 317
NspV TTCGAA 1 cut(s) 71
PauI GCGCGC 1 cut(s) 76
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PfeI GAWTC 4 cut(s) 137, 180, 475, 629
PkrI GCNGC 2 cut(s) 82, 259
PpuMI RGGWCCY 1 cut(s) 488
Psp5II RGGWCCY 1 cut(s) 488
Psp6I CCWGG 1 cut(s) 504
PspGI CCWGG 1 cut(s) 504
PspN4I GGNNCC 1 cut(s) 489
PspPI GGNCC 3 cut(s) 211, 488, 535
PspPPI RGGWCCY 1 cut(s) 488
PsuI RGATCY 1 cut(s) 307
PteI GCGCGC 1 cut(s) 76
RsaI GTAC 2 cut(s) 483, 548
RsaNI GTAC 2 cut(s) 482, 547
RseI CAYNNNNRTG 1 cut(s) 219
SaqAI TTAA 3 cut(s) 158, 335, 618
SatI GCNGC 2 cut(s) 81, 258
Sau3AI GATC 4 cut(s) 307, 519, 565, 634
Sau96I GGNCC 3 cut(s) 211, 488, 535
ScrFI CCNGG 2 cut(s) 351, 506
SduI GDGCHC 1 cut(s) 556
SetI ASST 9 cut(s) 31, 66, 91, 259, 504, 510, 537, 618, 695
SfaNI GCATC 2 cut(s) 225, 564
SfuI TTCGAA 1 cut(s) 71
SinI GGWCC 3 cut(s) 211, 488, 535
SmiMI CAYNNNNRTG 1 cut(s) 219
Sse9I AATT 4 cut(s) 332, 383, 467, 622
SsiI CCGC 1 cut(s) 81
StyD4I CCNGG 2 cut(s) 349, 504
TaaI ACNGT 2 cut(s) 460, 587
TaiI ACGT 1 cut(s) 66
TaqI TCGA 3 cut(s) 71, 183, 493
TasI AATT 4 cut(s) 332, 383, 467, 622
TatI WGTACW 1 cut(s) 546
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 4 cut(s) 137, 180, 475, 629
Tru1I TTAA 3 cut(s) 158, 335, 618
Tru9I TTAA 3 cut(s) 158, 335, 618
TscAI CASTG 1 cut(s) 575
TseI GCWGC 1 cut(s) 257
TspRI CASTG 1 cut(s) 575
VpaK11BI GGWCC 3 cut(s) 211, 488, 535
XapI RAATTY 1 cut(s) 383
XceI RCATGY 1 cut(s) 317
XmiI GTMKAC 2 cut(s) 114, 447
ZraI GACGTC 1 cut(s) 64
Zsp2I ATGCAT 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.