Rmu_sc0003572.1_g000003

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003572.1
OG0016719
Physical Location & Seq
Forward (+)
8989 .. 11886
2898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003572.1_g000003.1.cds

Sequence Viewer

Length: 606 bp
atgcctcgtgccacttcgaaaagaaaagcgccaccgtcgacctccgcctccgtcacctcctccgacgtcgtttcgactcgtcccggtaaaaaaggcaagaaggaggtagacaagataggcagcctctttgatgtgtacgcaaacaagaccttggacatgattgacccagaaggaattgtttctctttgttcgcatttgggagtggaccatactgatgtcagaatcctgatgcttgcttggtctaagaaagcactcccaggtctggaaaaagagttgatggccacacccaaatttgaagatttctatgcttttgcatttcaatactgcttgacagaagacaggcaaaagagtgttgatatcgaaactgtttgtgaattactgagtcttgtgcttggtcccaaataccgttcccaggttgatgtattaataaagtatctgaaggtccaaagtgagtacagggcattaaatgcggatcagtggagacatttttaccgtttttgcaaggagataagtttcccagatctcgaagactatgattctgatcaagcatggcctgtgataattgataatcttgttgaatggatgaaagagaacaagcaaagctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

201

Amino Acids

23.01

Weight (kDa)

5.97

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016719)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01430 FvH4_1g01430
malus_domestica MD15G1157200.v1.1
prunus_persica Prupe.7G258300_v2.0.a1 Prupe.7G258300_v2.0.a1
pyrus_communis pycom15g14100
rosa_chinensis RchiOBHm_Chr2g0086341
rosa_laevigata RLG00000015751
rosa_multiflora Rmu_sc0003572.1_g000003 Rmu_sc0004510.1_g000020
rosa_roxburghii Rroxscaffold_2G00154680
rosa_rugosa Rorug01G0466700
rosa_samantha Rh2AG016900
rosa_wichuraiana Rw2G001430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 69
AccI GTMKAC 2 cut(s) 38, 108
AciI CCGC 2 cut(s) 45, 470
AclWI GGATC 1 cut(s) 480
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 1 cut(s) 290
AcuI CTGAAG 1 cut(s) 458
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 2 cut(s) 137, 455
AfiI CCNNNNNNNGG 2 cut(s) 262, 412
AgsI TTSAA 3 cut(s) 296, 320, 578
AjnI CCWGG 2 cut(s) 256, 411
AluBI AGCT 1 cut(s) 603
AluI AGCT 1 cut(s) 603
Alw26I GTCTC 1 cut(s) 475
AlwI GGATC 1 cut(s) 480
AoxI GGCC 2 cut(s) 279, 551
ApeKI GCWGC 1 cut(s) 120
ApoI RAATTY 1 cut(s) 290
AseI ATTAAT 1 cut(s) 425
Asp700I GAANNNNTTC 1 cut(s) 178
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 3 cut(s) 205, 395, 442
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 46
AsuII TTCGAA 1 cut(s) 17
AvaII GGWCC 3 cut(s) 205, 395, 442
BalI TGGCCA 1 cut(s) 281
BauI CACGAG 1 cut(s) 6
BbsI GAAGAC 2 cut(s) 342, 534
BbvI GCAGC 1 cut(s) 132
BccI CCATC 1 cut(s) 271
BciT130I CCWGG 2 cut(s) 258, 413
BclI TGATCA 1 cut(s) 541
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 1 cut(s) 475
BfaI CTAG 1 cut(s) 604
BfoI RGCGCY 1 cut(s) 32
BglII AGATCT 1 cut(s) 520
BisI GCNGC 1 cut(s) 121
BlsI GCNGC 1 cut(s) 122
Bme1390I CCNGG 3 cut(s) 84, 258, 413
Bme18I GGWCC 3 cut(s) 205, 395, 442
BmgT120I GGNCC 3 cut(s) 205, 395, 442
BmiI GGNNCC 1 cut(s) 397
BmrFI CCNGG 3 cut(s) 84, 258, 413
BmsI GCATC 1 cut(s) 219
BpiI GAAGAC 2 cut(s) 342, 534
Bpu14I TTCGAA 1 cut(s) 17
BpuMI CCSGG 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 540
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 3 cut(s) 150, 256, 411
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 412
Bse8I GATNNNNATC 1 cut(s) 540
BseBI CCWGG 2 cut(s) 258, 413
BseDI CCNNGG 3 cut(s) 150, 256, 411
BseGI GGATG 1 cut(s) 588
BseJI GATNNNNATC 1 cut(s) 540
BseLI CCNNNNNNNGG 2 cut(s) 262, 412
BseMII CTCAG 1 cut(s) 371
BseRI GAGGAG 1 cut(s) 49
BseXI GCAGC 1 cut(s) 132
BshFI GGCC 2 cut(s) 281, 553
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 2 cut(s) 66, 381
BslI CCNNNNNNNGG 2 cut(s) 262, 412
BsmAI GTCTC 1 cut(s) 475
BsmFI GGGAC 2 cut(s) 66, 381
BsnI GGCC 2 cut(s) 281, 553
Bsp119I TTCGAA 1 cut(s) 17
Bsp143I GATC 3 cut(s) 472, 520, 541
BspACI CCGC 2 cut(s) 45, 470
BspANI GGCC 2 cut(s) 281, 553
BspCNI CTCAG 1 cut(s) 372
BspLI GGNNCC 1 cut(s) 397
BspPI GGATC 1 cut(s) 480
BspT104I TTCGAA 1 cut(s) 17
BssECI CCNNGG 3 cut(s) 150, 256, 411
BssMI GATC 3 cut(s) 472, 520, 541
BssNI GRCGYC 1 cut(s) 66
BssSI CACGAG 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 150
Bst2BI CACGAG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 258, 413
Bst4CI ACNGT 4 cut(s) 36, 367, 407, 494
BstACI GRCGYC 1 cut(s) 66
BstAPI GCANNNNNTGC 1 cut(s) 467
BstBI TTCGAA 1 cut(s) 17
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 2 cut(s) 243, 380
BstF5I GGATG 1 cut(s) 588
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 3 cut(s) 475, 523, 544
BstMAI GTCTC 1 cut(s) 475
BstMBI GATC 3 cut(s) 472, 520, 541
BstMWI GCNNNNNNNGC 1 cut(s) 467
BstNI CCWGG 2 cut(s) 258, 413
BstSCI CCNGG 3 cut(s) 82, 256, 411
BstV1I GCAGC 1 cut(s) 132
BstV2I GAAGAC 2 cut(s) 342, 534
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
BsuRI GGCC 2 cut(s) 281, 553
BtsCI GGATG 1 cut(s) 588
BtsIMutI CAGTG 1 cut(s) 482
Cac8I GCNNGC 1 cut(s) 234
CfoI GCGC 1 cut(s) 31
Cfr13I GGNCC 3 cut(s) 205, 395, 442
Csp6I GTAC 2 cut(s) 136, 454
CviAII CATG 2 cut(s) 157, 549
CviJI RGCY 4 cut(s) 123, 281, 553, 603
CviKI_1 RGCY 4 cut(s) 123, 281, 553, 603
CviQI GTAC 2 cut(s) 136, 454
DdeI CTNAG 2 cut(s) 243, 380
DpnI GATC 3 cut(s) 474, 522, 543
DpnII GATC 3 cut(s) 472, 520, 541
EaeI YGGCCR 1 cut(s) 279
EciI GGCGGA 1 cut(s) 34
Eco130I CCWWGG 1 cut(s) 150
Eco32I GATATC 1 cut(s) 358
Eco47I GGWCC 3 cut(s) 205, 395, 442
Eco57I CTGAAG 1 cut(s) 458
EcoRII CCWGG 2 cut(s) 256, 411
EcoRV GATATC 1 cut(s) 358
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 2 cut(s) 160, 552
FaiI YATR 5 cut(s) 158, 210, 306, 534, 550
FaqI GGGAC 2 cut(s) 66, 381
FatI CATG 2 cut(s) 156, 548
FbaI TGATCA 1 cut(s) 541
FblI GTMKAC 2 cut(s) 38, 108
Fnu4HI GCNGC 1 cut(s) 121
FokI GGATG 1 cut(s) 595
Fsp4HI GCNGC 1 cut(s) 121
FspBI CTAG 1 cut(s) 604
GlaI GCGC 1 cut(s) 30
GluI GCNGC 1 cut(s) 121
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 2 cut(s) 281, 553
HapII CCGG 1 cut(s) 84
HhaI GCGC 1 cut(s) 31
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 2 cut(s) 160, 552
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HinfI GANTC 4 cut(s) 76, 222, 382, 536
HpaII CCGG 1 cut(s) 84
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 4 cut(s) 39, 109, 136, 205
Hpy188I TCNGA 4 cut(s) 64, 221, 438, 541
Hpy188III TCNNGA 3 cut(s) 226, 263, 524
Hpy8I GTNNAC 4 cut(s) 39, 109, 136, 205
Hpy99I CGWCG 3 cut(s) 40, 68, 71
HpyAV CCTTC 3 cut(s) 94, 164, 433
HpyCH4III ACNGT 4 cut(s) 36, 367, 407, 494
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 2 cut(s) 314, 501
HpyF10VI GCNNNNNNNGC 1 cut(s) 467
HpyF3I CTNAG 2 cut(s) 243, 380
HpySE526I ACGT 1 cut(s) 66
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 2 cut(s) 160, 552
HspAI GCGC 1 cut(s) 29
Ksp22I TGATCA 1 cut(s) 541
Kzo9I GATC 3 cut(s) 472, 520, 541
Lsp1109I GCAGC 1 cut(s) 132
LweI GCATC 1 cut(s) 219
MaeI CTAG 1 cut(s) 604
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 1 cut(s) 52
MalI GATC 3 cut(s) 474, 522, 543
MboI GATC 3 cut(s) 472, 520, 541
MboII GAAGA 3 cut(s) 308, 347, 539
MflI RGATCY 1 cut(s) 520
MlsI TGGCCA 1 cut(s) 281
MluCI AATT 4 cut(s) 174, 290, 374, 561
MluNI TGGCCA 1 cut(s) 281
MlyI GAGTC 2 cut(s) 70, 391
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 7 cut(s) 15, 52, 58, 67, 70, 97, 134
Mox20I TGGCCA 1 cut(s) 281
MroXI GAANNNNTTC 1 cut(s) 178
MscI TGGCCA 1 cut(s) 281
MseI TTAA 2 cut(s) 425, 464
MslI CAYNNNNRTG 1 cut(s) 213
Msp20I TGGCCA 1 cut(s) 281
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 3 cut(s) 84, 258, 413
MvaI CCWGG 2 cut(s) 258, 413
MwoI GCNNNNNNNGC 1 cut(s) 467
NciI CCSGG 1 cut(s) 84
NdeII GATC 3 cut(s) 472, 520, 541
NlaIII CATG 2 cut(s) 160, 552
NlaIV GGNNCC 1 cut(s) 397
NmuCI GTSAC 1 cut(s) 52
NspV TTCGAA 1 cut(s) 17
PdmI GAANNNNTTC 1 cut(s) 178
PfeI GAWTC 2 cut(s) 222, 536
PkrI GCNGC 1 cut(s) 122
PleI GAGTC 2 cut(s) 70, 390
PpsI GAGTC 2 cut(s) 70, 390
PshBI ATTAAT 1 cut(s) 425
Psp6I CCWGG 2 cut(s) 256, 411
PspGI CCWGG 2 cut(s) 256, 411
PspN4I GGNNCC 1 cut(s) 397
PspPI GGNCC 3 cut(s) 205, 395, 442
PsuI RGATCY 1 cut(s) 520
RsaI GTAC 2 cut(s) 137, 455
RsaNI GTAC 2 cut(s) 136, 454
RseI CAYNNNNRTG 1 cut(s) 213
SalI GTCGAC 1 cut(s) 37
SaqAI TTAA 2 cut(s) 425, 464
SatI GCNGC 1 cut(s) 121
Sau3AI GATC 3 cut(s) 472, 520, 541
Sau96I GGNCC 3 cut(s) 205, 395, 442
SchI GAGTC 2 cut(s) 70, 391
ScrFI CCNGG 3 cut(s) 84, 258, 413
SetI ASST 9 cut(s) 44, 59, 69, 108, 152, 262, 417, 444, 605
SfaNI GCATC 1 cut(s) 219
SfuI TTCGAA 1 cut(s) 17
SinI GGWCC 3 cut(s) 205, 395, 442
SmiMI CAYNNNNRTG 1 cut(s) 213
Sse9I AATT 4 cut(s) 174, 290, 374, 561
SsiI CCGC 2 cut(s) 45, 470
SspMI CTAG 1 cut(s) 604
StyD4I CCNGG 3 cut(s) 82, 256, 411
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 4 cut(s) 36, 367, 407, 494
TaiI ACGT 1 cut(s) 69
TaqI TCGA 5 cut(s) 17, 38, 74, 360, 525
TasI AATT 4 cut(s) 174, 290, 374, 561
TatI WGTACW 1 cut(s) 453
TfiI GAWTC 2 cut(s) 222, 536
Tru1I TTAA 2 cut(s) 425, 464
Tru9I TTAA 2 cut(s) 425, 464
TscAI CASTG 1 cut(s) 482
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 1 cut(s) 120
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 1 cut(s) 599
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 1 cut(s) 482
VpaK11BI GGWCC 3 cut(s) 205, 395, 442
VspI ATTAAT 1 cut(s) 425
XapI RAATTY 1 cut(s) 290
XmiI GTMKAC 2 cut(s) 38, 108
XmnI GAANNNNTTC 1 cut(s) 178
XspI CTAG 1 cut(s) 604
ZraI GACGTC 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.