Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0015953

Physical Location & Seq

Genomic Coordinates Forward (+)

789347 .. 793776

Gene Structure 4430 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g01490.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2211 bp

ATGTCCGGGTGGGACGAAGGAGCGGTGTACTACAGCGACCAAGCGCAGTCGCTCGGGGGCGACACCGACGAACCCGGAAGCGCCGCCGCGAGCCGCCACTCGGTCCTCCAGAAATTCAAGGAGTTTCTCCGAAACTTCGAGTCCCAGTCAAGCACGAATGTCTTCCCTTACAGAGAGAGCCTCCTCCAAAACCCTAAGCACCTCGTCGTCGACATGGAGGATCTCGGCGACTTCGACTCCGATCTCCCCGCCAAGCTCCGCTCCGCCCCCGCCGATTACTTGCCCCTGTTTGAGAACGCCGCCGGGCAGGTTTTGGCAAATTTGAAGACGAAAGTGACCGGAAGTGAAGGCCAATTGGAAGAGCCGGTGCCGGAGGATGTTCAGATTTTGTTGACCTCCAGAGAAGATACAACTTCCATGCGCAAGCTTGGGGCACAAAGTATATCGAAGCTCATTAAGATATCTGGCATCACGATAGCTGCATCGAGGGTCAAAGCGAAGGCGACTTATGTTATCGTGATGTGTAAGAACTGTAAAAATGTGCAAAGGGTTCCTTGTCGACCAGGCCTTGGTGGTGCAATTGTCCCTCGCACGTGTAACCATATTCCTCAGCCTGGAGAGGAACCTTGTCCACTTGATCCATGGGTTGTGGTTCCTGATAAGAGCAAGTATGTTGATCAGCAAACATTAAAATTGCAAGAGAATCCAGAGGATGTGCCTACTGGAGAGCTTCCAAGAAATATGCTTCTATCTGTGGACCGCCATCTTGTTCAGACAATTGTGCCTGGCACAAGATTAACAATCATGGGAATTTATAGTATCTATCAAGCTTCCAATTCTTCCACCAACCACAAAGGAGCAGTTGCTGTTAGACAGCCCTACATCCGAGTAGTAGGAATTGAAGAAGCAAATGCTGCCAACTCAAGAGGTCCTGCTGCTTTCACAGCTGATGAGATAGAAGAATTTAAAAAATTTGCTGCAGAACCTGATGTATATAAAAATATATGCTCAAAGATTGCCCCTTCTATCTTTGGTCACGAAGATGTAAAGAAGGCCGTGGCTTGTCTTCTTTTTGGAGGATCAAGAAAGCATTTGCCTGATGGTGTGAAGCTAAGAGGTGATATAAATGTCTTGCTTTTGGGGGATCCATCTACTGCTAAATCACAGTTTCTCAAGTTTGTTGAGAAGACAGCTCCTGTAGCTGTTTATACTTCCGGGAAAGGCTCATCTGCGGCTGGTCTTACAGCATCTGTGATCAGAGATAACAGCTCTCGAGAGTTTTATCTTGAAGGAGGGGCCATGGTTTTGGCGGATGGAGGTGTTGTCTGTATTGATGAGTTTGATAAAATGAGACCGGAGGATAGGGTTGCTATTCATGAAGCGATGGAGCAGCAAACAATATCCATTGCCAAAGCAGGAATAACGACAGTTCTTAATTCTAGAACCTCTGTTCTTGCAGCTGCTAACCCTCCATCTGGACGTTATGATGATCTTAAGACCGCACAGGATAACATAGATCTGCAGACAACAATTCTTTCTAGATTTGATATGATCTTCATTGTTAAAGACATCAGGATGTATAGCCAGGATAAGATAATTGCAAGCCATGTAATAAAGGTTCATGCCTCTGCCGGGGCAGCAGTGGGTGACAAGAGGGAGGTTTCTAAGGAGAAGGAAGAGAATTGGCTGAAGAGGTACCTACAATACTGTCGAACCGAGTGTCATCCCCGTTTGTCAGAGTCTGCTTGCAAATCCCTGCAGAACCATTACGTCAAAATCAGACAGGACATGAGACAGCAGGCAAATGAAACGGGATCGGCTGCTGCAATACCAATCACTGTAAGGCAGCTTGAAGCTATAGTGAGGTTGAGTGAGTCGCTTGCAAAAATGAAACTATCCCATGTTGCTACCGAGGAAAATGTGACTGAAGCAGTAAGACTCTTTACCGTATCCACAATGGACGCAGCAAGGTCTGGAATTTATCAACAAGTCAATATTAGTCCCGAGATGGCAAATGAAATTAAGCAAGCTGAATCTCATATCAAGAGAAGAGTAGGCATCGGGAACCACATTTCGGAGCGAAAGTTGGTTGATGAGCTCACAAGAATGGGGATGAACGAGTCCATTGTGAGAAGAGCTCTTATAATTATGCACCAGAGAGATGAAGTCGAATACAAGGGAGAAAGACGCCTGATTCTACGTAAATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 ko05206 map03030 map04110 map04111 map04113 map05206

Pfam Domains

Protein Families

MCM MCM_N MCM_OB Mg_chelatase

Protein Analysis

737

Amino Acids

81.19

Weight (kDa)

7.9

Isoelectric Point (pI)

48.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	37 - 118	5.1e-12	MCM N-terminal domain
MCM_OB	PF17207	140 - 275	1.1e-34	MCM OB domain
MCM	PF00493	319 - 541	4.8e-102	MCM P-loop domain
MCM_lid	PF17855	562 - 651	8.3e-28	MCM AAA-lid domain
MCM5_C	PF21933	676 - 734	7.1e-17	MCM5, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0015953)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G07690 AT2G07690
fragaria_vesca	FvH4_1g01490
malus_domestica	MD02G1011900.v1.1 MD15G1157500.v1.1
prunus_persica	Prupe.7G252700_v2.0.a1
pyrus_communis	pycom15g14130
rosa_chinensis	RchiOBHm_Chr2g0086411
rosa_laevigata	RLG00000015756
rosa_multiflora	Rmu_sc0005127.1_g000006
rosa_roxburghii	Rroxscaffold_2G00154600
rosa_rugosa	Rorug01G0467200
rosa_samantha	Rh2AG017800 Rh2DG019100
rosa_wichuraiana	Rw2G001480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2144
Acc16I	TGCGCA	1 cut(s)	422
Acc36I	ACCTGC	1 cut(s)	298
Acc65I	GGTACC	1 cut(s)	1695
AccB1I	GGYRCC	2 cut(s)	367, 1695
AccB7I	CCANNNNNTGG	1 cut(s)	569
AccBSI	CCGCTC	2 cut(s)	23, 261
AccI	GTMKAC	2 cut(s)	210, 559
AccII	CGCG	1 cut(s)	89
AclWI	GGATC	6 cut(s)	228, 632, 1087, 1139, 1152, 1822
AcsI	RAATTY	6 cut(s)	113, 319, 810, 962, 971, 1977
AcuI	CTGAAG	2 cut(s)	1709, 1947
AcvI	CACGTG	1 cut(s)	594
AcyI	GRCGYC	1 cut(s)	2188
AfaI	GTAC	2 cut(s)	29, 1697
AfiI	CCNNNNNNNGG	6 cut(s)	100, 569, 614, 1475, 1632, 2074
AflII	CTTAAG	1 cut(s)	1493
AflIII	ACRYGT	1 cut(s)	593
AgsI	TTSAA	5 cut(s)	118, 325, 902, 1289, 1853
AjnI	CCWGG	4 cut(s)	562, 613, 784, 1584
Alw21I	GWGCWC	2 cut(s)	2100, 2140
Alw26I	GTCTC	2 cut(s)	1345, 1786
AlwI	GGATC	6 cut(s)	228, 632, 1087, 1139, 1152, 1822
AlwNI	CAGNNNCTG	5 cut(s)	866, 986, 1196, 1250, 1742
Ama87I	CYCGRG	3 cut(s)	53, 1272, 2003
AoxI	GGCC	4 cut(s)	349, 565, 1053, 1296
ApoI	RAATTY	6 cut(s)	113, 319, 810, 962, 971, 1977
ArsI	GACNNNNNNTTYG	2 cut(s)	1705, 1737
Asp700I	GAANNNNTTC	1 cut(s)	161
Asp718I	GGTACC	1 cut(s)	1695
AspLEI	GCGC	3 cut(s)	46, 83, 423
AspS9I	GGNCC	4 cut(s)	103, 757, 929, 1296
AsuC2I	CCSGG	5 cut(s)	7, 75, 304, 1216, 1633
AsuHPI	GGTGA	2 cut(s)	1130, 1658
AvaI	CYCGRG	3 cut(s)	53, 1272, 2003
AvaII	GGWCC	3 cut(s)	103, 757, 929
BaeGI	GKGCMC	1 cut(s)	436
BamHI	GGATCC	1 cut(s)	1144
BanI	GGYRCC	2 cut(s)	367, 1695
BanII	GRGCYC	2 cut(s)	2100, 2140
BbrPI	CACGTG	1 cut(s)	594
BbsI	GAAGAC	4 cut(s)	154, 332, 1058, 1193
Bbv12I	GWGCWC	2 cut(s)	2100, 2140
BbvCI	CCTCAGC	1 cut(s)	609
BccI	CCATC	7 cut(s)	771, 1094, 1156, 1307, 1378, 1480, 2002
BceAI	ACGGC	1 cut(s)	1040
BciT130I	CCWGG	4 cut(s)	564, 615, 786, 1586
BciVI	GTATCC	1 cut(s)	1960
BclI	TGATCA	2 cut(s)	676, 1254
BcnI	CCSGG	5 cut(s)	7, 75, 304, 1216, 1633
BcoDI	GTCTC	2 cut(s)	1345, 1786
BfaI	CTAG	2 cut(s)	1440, 1539
BfmI	CTRYAG	6 cut(s)	31, 978, 1197, 1520, 1757, 1857
BfoI	RGCGCY	1 cut(s)	84
BfrI	CTTAAG	1 cut(s)	1493
BfuAI	ACCTGC	1 cut(s)	298
BfuI	GTATCC	1 cut(s)	1960
BglII	AGATCT	1 cut(s)	1516
Bme1390I	CCNGG	9 cut(s)	7, 75, 304, 564, 615, 786, 1216, 1586, 1633
Bme18I	GGWCC	3 cut(s)	103, 757, 929
BmeT110I	CYCGRG	3 cut(s)	53, 1272, 2003
BmgT120I	GGNCC	4 cut(s)	103, 757, 929, 1296
BmiI	GGNNCC	8 cut(s)	369, 552, 624, 654, 1146, 1297, 1697, 2066
BmrFI	CCNGG	9 cut(s)	7, 75, 304, 564, 615, 786, 1216, 1586, 1633
BmrI	ACTGGG	1 cut(s)	139
BmsI	GCATC	4 cut(s)	477, 491, 1256, 2067
BmuI	ACTGGG	1 cut(s)	139
BpiI	GAAGAC	4 cut(s)	154, 332, 1058, 1193
BplI	GAGNNNNNCTC	4 cut(s)	165, 197, 2122, 2154
BpmI	CTGGAG	4 cut(s)	92, 382, 636, 744
Bpu10I	CCTNAGC	2 cut(s)	195, 609
BpuEI	CTTGAG	2 cut(s)	907, 1157
BpuMI	CCSGG	5 cut(s)	7, 75, 304, 1216, 1633
BsaAI	YACGTR	2 cut(s)	594, 2201
BsaHI	GRCGYC	1 cut(s)	2188
BsaI	GGTCTC	1 cut(s)	1345
BsaJI	CCNNGG	6 cut(s)	568, 641, 1056, 1299, 1632, 1911
BsaWI	WCCGGW	2 cut(s)	338, 1354
BsaXI	ACNNNNNCTCC	6 cut(s)	221, 251, 1308, 1338, 2069, 2099
Bsc4I	CCNNNNNNNGG	6 cut(s)	100, 569, 614, 1475, 1632, 2074
Bse118I	RCCGGY	1 cut(s)	364
Bse1I	ACTGG	2 cut(s)	145, 727
Bse3DI	GCAATG	1 cut(s)	1404
BseBI	CCWGG	4 cut(s)	564, 615, 786, 1586
BseDI	CCNNGG	6 cut(s)	568, 641, 1056, 1299, 1632, 1911
BseGI	GGATG	7 cut(s)	382, 718, 882, 1318, 1581, 1723, 2118
BseLI	CCNNNNNNNGG	6 cut(s)	100, 569, 614, 1475, 1632, 2074
BseMI	GCAATG	1 cut(s)	1404
BseMII	CTCAG	1 cut(s)	623
BseNI	ACTGG	2 cut(s)	145, 727
BseRI	GAGGAG	1 cut(s)	173
BseSI	GKGCMC	1 cut(s)	436
Bsh1236I	CGCG	1 cut(s)	89
BshFI	GGCC	4 cut(s)	351, 567, 1055, 1298
BshNI	GGYRCC	2 cut(s)	367, 1695
BsiHKAI	GWGCWC	2 cut(s)	2100, 2140
BsiHKCI	CYCGRG	3 cut(s)	53, 1272, 2003
BsiSI	CCGG	9 cut(s)	6, 75, 303, 339, 365, 371, 1215, 1355, 1632
BslFI	GGGAC	4 cut(s)	26, 127, 569, 1986
BslI	CCNNNNNNNGG	6 cut(s)	100, 569, 614, 1475, 1632, 2074
BsmAI	GTCTC	2 cut(s)	1345, 1786
BsmFI	GGGAC	4 cut(s)	26, 127, 569, 1986
BsnI	GGCC	4 cut(s)	351, 567, 1055, 1298
Bso31I	GGTCTC	1 cut(s)	1345
BsoBI	CYCGRG	3 cut(s)	53, 1272, 2003
Bsp1286I	GDGCHC	3 cut(s)	436, 2100, 2140
Bsp19I	CCATGG	2 cut(s)	641, 1299
BspANI	GGCC	4 cut(s)	351, 567, 1055, 1298
BspCNI	CTCAG	1 cut(s)	622
BspFNI	CGCG	1 cut(s)	89
BspHI	TCATGA	1 cut(s)	1375
BspLI	GGNNCC	8 cut(s)	369, 552, 624, 654, 1146, 1297, 1697, 2066
BspMAI	CTGCAG	3 cut(s)	982, 1524, 1761
BspMI	ACCTGC	1 cut(s)	298
BspPI	GGATC	6 cut(s)	228, 632, 1087, 1139, 1152, 1822
BspQI	GCTCTTC	2 cut(s)	354, 2128
BspT107I	GGYRCC	2 cut(s)	367, 1695
BspTI	CTTAAG	1 cut(s)	1493
BspTNI	GGTCTC	1 cut(s)	1345
BsrBI	CCGCTC	2 cut(s)	23, 261
BsrDI	GCAATG	1 cut(s)	1404
BsrFI	RCCGGY	1 cut(s)	364
BsrI	ACTGG	2 cut(s)	145, 727
BssAI	RCCGGY	1 cut(s)	364
BssECI	CCNNGG	6 cut(s)	568, 641, 1056, 1299, 1632, 1911
BssNI	GRCGYC	1 cut(s)	2188
BssT1I	CCWWGG	3 cut(s)	568, 641, 1299
Bst2UI	CCWGG	4 cut(s)	564, 615, 786, 1586
Bst4CI	ACNGT	6 cut(s)	533, 1167, 1429, 1709, 1840, 1948
Bst6I	CTCTTC	5 cut(s)	354, 1671, 1685, 2044, 2128
BstACI	GRCGYC	1 cut(s)	2188
BstAFI	CTTAAG	1 cut(s)	1493
BstAPI	GCANNNNNTGC	1 cut(s)	914
BstBAI	YACGTR	2 cut(s)	594, 2201
BstC8I	GCNNGC	7 cut(s)	91, 425, 1603, 1747, 1800, 1881, 2028
BstDEI	CTNAG	4 cut(s)	195, 609, 1112, 1665
BstDSI	CCRYGG	3 cut(s)	641, 1056, 1299
BstF5I	GGATG	7 cut(s)	382, 718, 882, 1318, 1581, 1723, 2118
BstFNI	CGCG	1 cut(s)	89
BstH2I	RGCGCY	1 cut(s)	84
BstHHI	GCGC	3 cut(s)	46, 83, 423
BstMAI	GTCTC	2 cut(s)	1345, 1786
BstMWI	GCNNNNNNNGC	4 cut(s)	914, 944, 1199, 1637
BstNI	CCWGG	4 cut(s)	564, 615, 786, 1586
BstSCI	CCNGG	9 cut(s)	5, 73, 302, 562, 613, 784, 1214, 1584, 1631
BstSFI	CTRYAG	6 cut(s)	31, 978, 1197, 1520, 1757, 1857
BstSLI	GKGCMC	1 cut(s)	436
BstSNI	TACGTA	1 cut(s)	2201
BstUI	CGCG	1 cut(s)	89
BstV2I	GAAGAC	4 cut(s)	154, 332, 1058, 1193
BstX2I	RGATCY	3 cut(s)	220, 1144, 1516
BstXI	CCANNNNNNTGG	1 cut(s)	1306
BstYI	RGATCY	3 cut(s)	220, 1144, 1516
BsuI	GTATCC	1 cut(s)	1960
BsuRI	GGCC	4 cut(s)	351, 567, 1055, 1298
BtgI	CCRYGG	3 cut(s)	641, 1056, 1299
BtgZI	GCGATG	1 cut(s)	1397
BtsCI	GGATG	7 cut(s)	382, 718, 882, 1318, 1581, 1723, 2118
BtsI	GCAGTG	1 cut(s)	1647
BtsIMutI	CAGTG	2 cut(s)	1647, 1836
BveI	ACCTGC	1 cut(s)	298
Cac8I	GCNNGC	7 cut(s)	91, 425, 1603, 1747, 1800, 1881, 2028
CaiI	CAGNNNCTG	5 cut(s)	866, 986, 1196, 1250, 1742
CciI	TCATGA	1 cut(s)	1375
CfoI	GCGC	3 cut(s)	46, 83, 423
Cfr10I	RCCGGY	1 cut(s)	364
Cfr13I	GGNCC	4 cut(s)	103, 757, 929, 1296
CseI	GACGC	2 cut(s)	1970, 2196
Csp6I	GTAC	2 cut(s)	28, 1696
CviQI	GTAC	2 cut(s)	28, 1696
DdeI	CTNAG	4 cut(s)	195, 609, 1112, 1665
DraI	TTTAAA	1 cut(s)	967
Eam1104I	CTCTTC	5 cut(s)	354, 1671, 1685, 2044, 2128
EarI	CTCTTC	5 cut(s)	354, 1671, 1685, 2044, 2128
EciI	GGCGGA	2 cut(s)	253, 1325
Ecl136II	GAGCTC	2 cut(s)	2098, 2138
Eco105I	TACGTA	1 cut(s)	2201
Eco130I	CCWWGG	3 cut(s)	568, 641, 1299
Eco147I	AGGCCT	1 cut(s)	567
Eco24I	GRGCYC	2 cut(s)	2100, 2140
Eco31I	GGTCTC	1 cut(s)	1345
Eco32I	GATATC	1 cut(s)	462
Eco47I	GGWCC	3 cut(s)	103, 757, 929
Eco53kI	GAGCTC	2 cut(s)	2098, 2138
Eco57I	CTGAAG	2 cut(s)	1709, 1947
Eco72I	CACGTG	1 cut(s)	594
Eco88I	CYCGRG	3 cut(s)	53, 1272, 2003
EcoICRI	GAGCTC	2 cut(s)	2098, 2138
EcoO109I	RGGNCCY	1 cut(s)	929
EcoRII	CCWGG	4 cut(s)	562, 613, 784, 1584
EcoRV	GATATC	1 cut(s)	462
EcoT14I	CCWWGG	3 cut(s)	568, 641, 1299
EcoT38I	GRGCYC	2 cut(s)	2100, 2140
ErhI	CCWWGG	3 cut(s)	568, 641, 1299
FalI	AAGNNNNNCTT	2 cut(s)	538, 570
FaqI	GGGAC	4 cut(s)	26, 127, 569, 1986
FauI	CCCGC	2 cut(s)	256, 277
FbaI	TGATCA	2 cut(s)	676, 1254
FblI	GTMKAC	2 cut(s)	210, 559
FokI	GGATG	7 cut(s)	389, 725, 869, 1325, 1588, 1710, 2125
FriOI	GRGCYC	2 cut(s)	2100, 2140
FspBI	CTAG	2 cut(s)	1440, 1539
FspI	TGCGCA	1 cut(s)	422
GlaI	GCGC	3 cut(s)	45, 82, 422
GsuI	CTGGAG	4 cut(s)	92, 382, 636, 744
HaeII	RGCGCY	1 cut(s)	84
HaeIII	GGCC	4 cut(s)	351, 567, 1055, 1298
HapII	CCGG	9 cut(s)	6, 75, 303, 339, 365, 371, 1215, 1355, 1632
HgaI	GACGC	2 cut(s)	1970, 2196
HhaI	GCGC	3 cut(s)	46, 83, 423
Hin1I	GRCGYC	1 cut(s)	2188
Hin6I	GCGC	3 cut(s)	44, 81, 421
HinP1I	GCGC	3 cut(s)	44, 81, 421
HincII	GTYRAC	3 cut(s)	211, 393, 560
HindII	GTYRAC	3 cut(s)	211, 393, 560
HindIII	AAGCTT	2 cut(s)	425, 828
HinfI	GANTC	9 cut(s)	140, 236, 703, 1739, 1874, 1938, 2033, 2120, 2194
HpaII	CCGG	9 cut(s)	6, 75, 303, 339, 365, 371, 1215, 1355, 1632
HphI	GGTGA	2 cut(s)	1130, 1658
Hpy166II	GTNNAC	6 cut(s)	28, 211, 393, 560, 632, 757
Hpy188I	TCNGA	9 cut(s)	131, 241, 384, 774, 887, 1259, 1738, 1781, 2077
Hpy8I	GTNNAC	6 cut(s)	28, 211, 393, 560, 632, 757
Hpy99I	CGWCG	3 cut(s)	71, 209, 212
HpyAV	CCTTC	7 cut(s)	11, 341, 493, 1032, 1045, 1283, 1666
HpyCH4III	ACNGT	6 cut(s)	533, 1167, 1429, 1709, 1840, 1948
HpyCH4IV	ACGT	4 cut(s)	593, 1480, 1770, 2200
HpyF10VI	GCNNNNNNNGC	4 cut(s)	914, 944, 1199, 1637
HpyF3I	CTNAG	4 cut(s)	195, 609, 1112, 1665
HpySE526I	ACGT	4 cut(s)	593, 1480, 1770, 2200
Hsp92I	GRCGYC	1 cut(s)	2188
HspAI	GCGC	3 cut(s)	44, 81, 421
KpnI	GGTACC	1 cut(s)	1699
Ksp22I	TGATCA	2 cut(s)	676, 1254
LguI	GCTCTTC	2 cut(s)	354, 2128
LmnI	GCTCC	7 cut(s)	20, 261, 266, 857, 1198, 1387, 2077
LweI	GCATC	4 cut(s)	477, 491, 1256, 2067
MaeI	CTAG	2 cut(s)	1440, 1539
MaeII	ACGT	4 cut(s)	593, 1480, 1770, 2200
MaeIII	GTNAC	5 cut(s)	334, 596, 1034, 1646, 1921
MbiI	CCGCTC	2 cut(s)	23, 261
MfeI	CAATTG	3 cut(s)	353, 579, 777
MflI	RGATCY	3 cut(s)	220, 1144, 1516
MhlI	GDGCHC	3 cut(s)	436, 2100, 2140
MlyI	GAGTC	6 cut(s)	149, 230, 1748, 1883, 1932, 2129
MroXI	GAANNNNTTC	1 cut(s)	161
MseI	TTAA	8 cut(s)	456, 689, 797, 966, 1434, 1494, 1563, 2022
MslI	CAYNNNNRTG	3 cut(s)	1041, 1574, 2105
MspA1I	CMGCKG	2 cut(s)	947, 1460
MspCI	CTTAAG	1 cut(s)	1493
MspI	CCGG	9 cut(s)	6, 75, 303, 339, 365, 371, 1215, 1355, 1632
MspR9I	CCNGG	9 cut(s)	7, 75, 304, 564, 615, 786, 1216, 1586, 1633
MunI	CAATTG	3 cut(s)	353, 579, 777
MvaI	CCWGG	4 cut(s)	564, 615, 786, 1586
MvnI	CGCG	1 cut(s)	89
MwoI	GCNNNNNNNGC	4 cut(s)	914, 944, 1199, 1637
NciI	CCSGG	5 cut(s)	7, 75, 304, 1216, 1633
NcoI	CCATGG	2 cut(s)	641, 1299
NlaIV	GGNNCC	8 cut(s)	369, 552, 624, 654, 1146, 1297, 1697, 2066
NmeAIII	GCCGAG	1 cut(s)	204
NmuCI	GTSAC	4 cut(s)	334, 1034, 1646, 1921
NsbI	TGCGCA	1 cut(s)	422
PaeR7I	CTCGAG	1 cut(s)	1272
PagI	TCATGA	1 cut(s)	1375
PceI	AGGCCT	1 cut(s)	567
PciSI	GCTCTTC	2 cut(s)	354, 2128
PcsI	WCGNNNNNNNCGW	1 cut(s)	231
PdmI	GAANNNNTTC	1 cut(s)	161
PfeI	GAWTC	3 cut(s)	703, 2033, 2194
PflMI	CCANNNNNTGG	1 cut(s)	569
PfoI	TCCNGGA	1 cut(s)	1214
PleI	GAGTC	6 cut(s)	148, 230, 1747, 1882, 1932, 2128
PmaCI	CACGTG	1 cut(s)	594
PmlI	CACGTG	1 cut(s)	594
PpsI	GAGTC	6 cut(s)	148, 230, 1747, 1882, 1932, 2128
Ppu21I	YACGTR	2 cut(s)	594, 2201
PpuMI	RGGWCCY	1 cut(s)	929
PsiI	TTATAA	1 cut(s)	2144
Psp124BI	GAGCTC	2 cut(s)	2100, 2140
Psp5II	RGGWCCY	1 cut(s)	929
Psp6I	CCWGG	4 cut(s)	562, 613, 784, 1584
PspCI	CACGTG	1 cut(s)	594
PspGI	CCWGG	4 cut(s)	562, 613, 784, 1584
PspN4I	GGNNCC	8 cut(s)	369, 552, 624, 654, 1146, 1297, 1697, 2066
PspPI	GGNCC	4 cut(s)	103, 757, 929, 1296
PspPPI	RGGWCCY	1 cut(s)	929
PsrI	GAACNNNNNNTAC	2 cut(s)	1602, 1634
PstI	CTGCAG	3 cut(s)	982, 1524, 1761
PstNI	CAGNNNCTG	5 cut(s)	866, 986, 1196, 1250, 1742
PsuI	RGATCY	3 cut(s)	220, 1144, 1516
PvuII	CAGCTG	2 cut(s)	947, 1460
RsaI	GTAC	2 cut(s)	29, 1697
RsaNI	GTAC	2 cut(s)	28, 1696
RseI	CAYNNNNRTG	3 cut(s)	1041, 1574, 2105
SacI	GAGCTC	2 cut(s)	2100, 2140
SalI	GTCGAC	2 cut(s)	209, 558
SapI	GCTCTTC	2 cut(s)	354, 2128
SaqAI	TTAA	8 cut(s)	456, 689, 797, 966, 1434, 1494, 1563, 2022
Sau96I	GGNCC	4 cut(s)	103, 757, 929, 1296
SchI	GAGTC	6 cut(s)	149, 230, 1748, 1883, 1932, 2129
ScrFI	CCNGG	9 cut(s)	7, 75, 304, 564, 615, 786, 1216, 1586, 1633
SduI	GDGCHC	3 cut(s)	436, 2100, 2140
SfaNI	GCATC	4 cut(s)	477, 491, 1256, 2067
SfcI	CTRYAG	6 cut(s)	31, 978, 1197, 1520, 1757, 1857
Sfr274I	CTCGAG	1 cut(s)	1272
SinI	GGWCC	3 cut(s)	103, 757, 929
SlaI	CTCGAG	1 cut(s)	1272
SmiMI	CAYNNNNRTG	3 cut(s)	1041, 1574, 2105
SmlI	CTYRAG	4 cut(s)	922, 1172, 1272, 1493
SmoI	CTYRAG	4 cut(s)	922, 1172, 1272, 1493
SnaBI	TACGTA	1 cut(s)	2201
SseBI	AGGCCT	1 cut(s)	567
SspI	AATATT	1 cut(s)	1996
SspMI	CTAG	2 cut(s)	1440, 1539
SstI	GAGCTC	2 cut(s)	2100, 2140
StuI	AGGCCT	1 cut(s)	567
StyD4I	CCNGG	9 cut(s)	5, 73, 302, 562, 613, 784, 1214, 1584, 1631
StyI	CCWWGG	3 cut(s)	568, 641, 1299
TaaI	ACNGT	6 cut(s)	533, 1167, 1429, 1709, 1840, 1948
TaiI	ACGT	4 cut(s)	596, 1483, 1773, 2203
TaqI	TCGA	9 cut(s)	138, 210, 234, 446, 485, 559, 1273, 1711, 2169
TaqII	GACCGA	1 cut(s)	91
TatI	WGTACW	1 cut(s)	27
TauI	GCSGC	5 cut(s)	86, 89, 96, 302, 1235
TfiI	GAWTC	3 cut(s)	703, 2033, 2194
Tru1I	TTAA	8 cut(s)	456, 689, 797, 966, 1434, 1494, 1563, 2022
Tru9I	TTAA	8 cut(s)	456, 689, 797, 966, 1434, 1494, 1563, 2022
TscAI	CASTG	2 cut(s)	1647, 1843
TseFI	GTSAC	4 cut(s)	334, 1034, 1646, 1921
Tsp45I	GTSAC	4 cut(s)	334, 1034, 1646, 1921
TspDTI	ATGAA	9 cut(s)	1364, 1392, 1546, 1610, 1821, 1904, 2031, 2129, 2178
TspRI	CASTG	2 cut(s)	1647, 1843
Van91I	CCANNNNNTGG	1 cut(s)	569
Vha464I	CTTAAG	1 cut(s)	1493
VpaK11BI	GGWCC	3 cut(s)	103, 757, 929
XapI	RAATTY	6 cut(s)	113, 319, 810, 962, 971, 1977
XbaI	TCTAGA	2 cut(s)	1439, 1538
XcmI	CCANNNNNNNNNTGG	1 cut(s)	639
XhoI	CTCGAG	1 cut(s)	1272
XmiI	GTMKAC	2 cut(s)	210, 559
XmnI	GAANNNNTTC	1 cut(s)	161
XspI	CTAG	2 cut(s)	1440, 1539

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.