Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0015953

Physical Location & Seq

Genomic Coordinates Reverse (-)

1239816 .. 1244470

Gene Structure 4655 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G001480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2178 bp

ATGTCGGGGTGGGATGAAGGAGCGGTGTACTACAGCGACCAAGCGAGTCGCTGGGCGGCGACACCGACGACCCCGGAAAAATTCAAGGAGTTCATCCGAAACTTCGAGACCCAGTCAAGCACGAATGTCTTCCCTTACAGAGAGAGCCTCCTCCACAACCCTAAGCACCTCGTCGTCGACATGGAGGACCTCGGCGACTTCGACTCCGATCTCCCCGCCAAGCTCCGCTCCGCCCCCGCCGATTACTTGCCTCTGTTTGAGAACGCCGCCGGACAGGTTTTGGCGAATTTGAAGACGAAGGTGGCCGGAAGCGAAGGCCAATTGGAGGAACCGGTGCCGGAGGATGTTCAGATTTTGTTGACCTCCAGAGAAGATACAACTTCCATGCGCAAGCTTGGGGCTCAAAGTATATCAAAACTCATCAAGATATCTGGGATCACGATAGCTGCATCAAGAGTCAAGGCAAAGGCTACTTATGTGATTGTGATGTGTAAGAACTGTAAAAATGTGCAAAGGGTTCCTTGTCGACCAGGCCTTGGTGGGGCAATTGTACCTCGCACGTGTAACCATATTCCTCAGCCTGGAGAAGAACCTTGTCCACTTGATCCATGGGTTGTGGTTCCTGATAAGAGCAAGTATGTTGATCAGCAGACCTTAAAATTGCAAGAGAATCCGGAGGATGTGCCTACTGGAGAGCTTCCAAGAAATATGCTTCTATCTGTGGACCGCCATCTTGTTCAGACAATTGTGCCTGGCACAAGATTAACAATCATGGGAATTTATAGTATCTATCAAGCTTCCAATTCTTCCACCAACCACAAAGGAGCAGTTGCTGTTAGACAGCCTTACATCAGAGTAGTAGGAATCGAAGAAGCAAATGATGCCAACTCAAGAGGTCCCGCTGCTTTCACAGCTGAAGAGATAGAAGAATTTAAGAAATTTGCTTCAGAACCTGATGTATATAAAAGCATATGCTCAAAGATTGCCCCCTCTATCTTTGGTCACGAAGATGTAAAGAAGGCCATGGCTTGTCTTCTTTTTGGGGGATCTAGAAAGCATTTGCCTGATGGTGTGAAGCTAAGAGGTGATATCAATGTCTTACTTTTGGGGGATCCATCTACTGCTAAATCACAGTTTCTCAAGTTTGTTGAGAAAACAGCTCCTGTAGCTGTTTATACTTCCGGGAAAGGCTCATCTGCAGCTGGTCTTACAGCTTCTGTGATCAGAGATAACAGCTCTCGAGAGTTTTATCTTGAAGGAGGAGCCATGGTTTTGGCAGATGGAGGTGTTGTGTGTATTGATGAGTTTGACAAAATGAGACCGGAGGACAGGGTTGCCATTCATGAAGCCATGGAGCAGCAAACCATTTCCATTGCCAAAGCAGGAATAACAACAGTTCTTAATTCCAGAACCTCTGTTCTTGCAGCTGCTAACCCTCCATCTGGACGTTATGATGATCTTAAGACTGCTCAGGATAACATAGATCTGCAGACAACAATTCTTTCTAGATTTGATTTGATCTTCATTGTTAAAGACATCAGGATGTATAGTCAGGACAAGATAATAGCAAGTCATGTAATAAAGGTTCATGCATCTGCTGGGGCAGCAGTGGGTGATAAGAGGGAGGTTTCTAAGGAGAAAGAAGAGAATTGGCTGAAGAGGTACCTTCAATACTGTCGAACTGAGTGTCATCCCCGTTTGTCAGAGTCTGCATCCAAATCACTGCAGAACCATTATGTCAAAATTAGACAGGACATGAGACAGCAGGCAAATGAAACTGGAGAGGCTGCTGCAATACCAATCACTGTAAGGCAGCTTGAAGCTATAGTGAGGTTGAGTGAGTCGCTTGCAAAAATGAAACTGTCCCATGTTGCTACCGAGGAGAACGTGACTGAAGCAGTAAGACTTTTTACTGTATCCACAATGGATGCAGCAAGATCTGGAATTTATCAACAAGTCAATATTAGTCCTGAGATGGCAAATGAAATTAAGCAAGCTGAATCTCATATTAAGAGAAGAGTAGGCATTGGGAACCACATTTCGGAGAGAAAGTTGGTTGATGAGCTCACAAGAATGGGGATGAATGAATCCATTGTGAGAAGAGCTCTTATAATTATGCACCAGAGAGATGAAGTCGAATACAAGGGAGAAAGACGCCTGATTTTACGTAAATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 ko05206 map03030 map04110 map04111 map04113 map05206

Pfam Domains

Protein Families

MCM MCM_N MCM_OB Mg_chelatase

Protein Analysis

725

Amino Acids

80.3

Weight (kDa)

7.92

Isoelectric Point (pI)

47.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	26 - 107	1.2e-11	MCM N-terminal domain
MCM_OB	PF17207	129 - 264	1.1e-34	MCM OB domain
MCM	PF00493	308 - 530	9e-102	MCM P-loop domain
MCM_lid	PF17855	551 - 640	4.6e-28	MCM AAA-lid domain
MCM5_C	PF21933	665 - 723	7e-17	MCM5, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0015953)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G07690 AT2G07690
fragaria_vesca	FvH4_1g01490
malus_domestica	MD02G1011900.v1.1 MD15G1157500.v1.1
prunus_persica	Prupe.7G252700_v2.0.a1
pyrus_communis	pycom15g14130
rosa_chinensis	RchiOBHm_Chr2g0086411
rosa_laevigata	RLG00000015756
rosa_multiflora	Rmu_sc0005127.1_g000006
rosa_roxburghii	Rroxscaffold_2G00154600
rosa_rugosa	Rorug01G0467200
rosa_samantha	Rh2AG017800 Rh2DG019100
rosa_wichuraiana	Rw2G001480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2111
Acc16I	TGCGCA	1 cut(s)	389
Acc65I	GGTACC	1 cut(s)	1662
AccB1I	GGYRCC	2 cut(s)	334, 1662
AccB7I	CCANNNNNTGG	1 cut(s)	536
AccBSI	CCGCTC	2 cut(s)	23, 228
AccI	GTMKAC	2 cut(s)	177, 526
AccIII	TCCGGA	1 cut(s)	673
AciI	CCGC	9 cut(s)	23, 56, 216, 226, 231, 237, 267, 727, 900
AclWI	GGATC	5 cut(s)	443, 599, 1054, 1106, 1119
AcoI	YGGCCR	1 cut(s)	303
AcsI	RAATTY	6 cut(s)	80, 286, 777, 929, 938, 1944
AcuI	CTGAAG	4 cut(s)	930, 936, 1676, 1914
AcvI	CACGTG	1 cut(s)	561
AcyI	GRCGYC	1 cut(s)	2155
AfaI	GTAC	3 cut(s)	29, 552, 1664
AfiI	CCNNNNNNNGG	6 cut(s)	325, 536, 541, 581, 1442, 2041
AflII	CTTAAG	1 cut(s)	1460
AflIII	ACRYGT	1 cut(s)	560
AgeI	ACCGGT	1 cut(s)	331
AgsI	TTSAA	5 cut(s)	85, 292, 1256, 1670, 1820
AjnI	CCWGG	3 cut(s)	529, 580, 751
Alw21I	GWGCWC	2 cut(s)	2067, 2107
Alw26I	GTCTC	3 cut(s)	101, 1312, 1753
AlwI	GGATC	5 cut(s)	443, 599, 1054, 1106, 1119
AlwNI	CAGNNNCTG	5 cut(s)	833, 953, 1163, 1217, 1709
Ama87I	CYCGRG	1 cut(s)	1239
Aor13HI	TCCGGA	1 cut(s)	673
AoxI	GGCC	4 cut(s)	303, 316, 532, 1020
ApoI	RAATTY	6 cut(s)	80, 286, 777, 929, 938, 1944
ArsI	GACNNNNNNTTYG	2 cut(s)	1672, 1704
AsiGI	ACCGGT	1 cut(s)	331
Asp700I	GAANNNNTTC	1 cut(s)	128
Asp718I	GGTACC	1 cut(s)	1662
AspLEI	GCGC	1 cut(s)	390
AspS9I	GGNCC	3 cut(s)	187, 724, 896
AsuC2I	CCSGG	2 cut(s)	74, 1183
AsuHPI	GGTGA	2 cut(s)	1097, 1625
AvaI	CYCGRG	1 cut(s)	1239
AvaII	GGWCC	3 cut(s)	187, 724, 896
BamHI	GGATCC	1 cut(s)	1111
BanI	GGYRCC	2 cut(s)	334, 1662
BanII	GRGCYC	3 cut(s)	403, 2067, 2107
BbrPI	CACGTG	1 cut(s)	561
BbsI	GAAGAC	3 cut(s)	121, 299, 1025
Bbv12I	GWGCWC	2 cut(s)	2067, 2107
BbvCI	CCTCAGC	1 cut(s)	576
BccI	CCATC	6 cut(s)	738, 1061, 1123, 1274, 1447, 1969
BciT130I	CCWGG	3 cut(s)	531, 582, 753
BciVI	GTATCC	1 cut(s)	1927
BclI	TGATCA	2 cut(s)	643, 1221
BcnI	CCSGG	2 cut(s)	74, 1183
BcoDI	GTCTC	3 cut(s)	101, 1312, 1753
BfaI	CTAG	2 cut(s)	1050, 1506
BfmI	CTRYAG	6 cut(s)	31, 1164, 1197, 1487, 1724, 1824
BfrI	CTTAAG	1 cut(s)	1460
BfuI	GTATCC	1 cut(s)	1927
BglII	AGATCT	2 cut(s)	1483, 1937
Bme1390I	CCNGG	5 cut(s)	74, 531, 582, 753, 1183
Bme18I	GGWCC	3 cut(s)	187, 724, 896
BmeT110I	CYCGRG	1 cut(s)	1239
BmgT120I	GGNCC	3 cut(s)	187, 724, 896
BmiI	GGNNCC	9 cut(s)	330, 336, 519, 621, 898, 1113, 1264, 1664, 2033
BmrFI	CCNGG	5 cut(s)	74, 531, 582, 753, 1183
BmrI	ACTGGG	1 cut(s)	106
BmsI	GCATC	5 cut(s)	458, 871, 1601, 1721, 1918
BmuI	ACTGGG	1 cut(s)	106
BpiI	GAAGAC	3 cut(s)	121, 299, 1025
BplI	GAGNNNNNCTC	4 cut(s)	132, 164, 2089, 2121
BpmI	CTGGAG	4 cut(s)	349, 603, 711, 1800
Bpu10I	CCTNAGC	3 cut(s)	162, 576, 1470
BpuEI	CTTGAG	2 cut(s)	874, 1124
BpuMI	CCSGG	2 cut(s)	74, 1183
BsaAI	YACGTR	2 cut(s)	561, 2168
BsaHI	GRCGYC	1 cut(s)	2155
BsaI	GGTCTC	2 cut(s)	101, 1312
BsaJI	CCNNGG	8 cut(s)	72, 190, 535, 608, 1023, 1266, 1350, 1878
BsaWI	WCCGGW	3 cut(s)	331, 673, 1321
BsaXI	ACNNNNNCTCC	8 cut(s)	188, 218, 1254, 1275, 1284, 1305, 2036, 2066
Bsc4I	CCNNNNNNNGG	6 cut(s)	325, 536, 541, 581, 1442, 2041
Bse118I	RCCGGY	1 cut(s)	331
Bse1I	ACTGG	3 cut(s)	112, 694, 1783
Bse3DI	GCAATG	1 cut(s)	1371
BseAI	TCCGGA	1 cut(s)	673
BseBI	CCWGG	3 cut(s)	531, 582, 753
BseDI	CCNNGG	8 cut(s)	72, 190, 535, 608, 1023, 1266, 1350, 1878
BseGI	GGATG	9 cut(s)	19, 93, 349, 685, 1548, 1690, 1712, 1933, 2085
BseLI	CCNNNNNNNGG	6 cut(s)	325, 536, 541, 581, 1442, 2041
BseMI	GCAATG	1 cut(s)	1371
BseMII	CTCAG	4 cut(s)	590, 1484, 1674, 1962
BseNI	ACTGG	3 cut(s)	112, 694, 1783
BseRI	GAGGAG	3 cut(s)	140, 1275, 1895
BseYI	CCCAGC	2 cut(s)	51, 1598
BshFI	GGCC	4 cut(s)	305, 318, 534, 1022
BshNI	GGYRCC	2 cut(s)	334, 1662
BshTI	ACCGGT	1 cut(s)	331
BsiHKAI	GWGCWC	2 cut(s)	2067, 2107
BsiHKCI	CYCGRG	1 cut(s)	1239
BsiSI	CCGG	8 cut(s)	74, 270, 306, 332, 338, 674, 1182, 1322
BslFI	GGGAC	2 cut(s)	882, 1849
BslI	CCNNNNNNNGG	6 cut(s)	325, 536, 541, 581, 1442, 2041
BsmAI	GTCTC	3 cut(s)	101, 1312, 1753
BsmFI	GGGAC	2 cut(s)	882, 1849
BsnI	GGCC	4 cut(s)	305, 318, 534, 1022
Bso31I	GGTCTC	2 cut(s)	101, 1312
BsoBI	CYCGRG	1 cut(s)	1239
Bsp1286I	GDGCHC	3 cut(s)	403, 2067, 2107
Bsp13I	TCCGGA	1 cut(s)	673
Bsp19I	CCATGG	4 cut(s)	608, 1023, 1266, 1350
BspACI	CCGC	9 cut(s)	23, 56, 216, 226, 231, 237, 267, 727, 900
BspANI	GGCC	4 cut(s)	305, 318, 534, 1022
BspCNI	CTCAG	4 cut(s)	589, 1483, 1675, 1963
BspEI	TCCGGA	1 cut(s)	673
BspHI	TCATGA	1 cut(s)	1342
BspLI	GGNNCC	9 cut(s)	330, 336, 519, 621, 898, 1113, 1264, 1664, 2033
BspMAI	CTGCAG	3 cut(s)	1201, 1491, 1728
BspPI	GGATC	5 cut(s)	443, 599, 1054, 1106, 1119
BspQI	GCTCTTC	1 cut(s)	2095
BspT107I	GGYRCC	2 cut(s)	334, 1662
BspTI	CTTAAG	1 cut(s)	1460
BspTNI	GGTCTC	2 cut(s)	101, 1312
BsrBI	CCGCTC	2 cut(s)	23, 228
BsrDI	GCAATG	1 cut(s)	1371
BsrFI	RCCGGY	1 cut(s)	331
BsrI	ACTGG	3 cut(s)	112, 694, 1783
BssAI	RCCGGY	1 cut(s)	331
BssECI	CCNNGG	8 cut(s)	72, 190, 535, 608, 1023, 1266, 1350, 1878
BssNI	GRCGYC	1 cut(s)	2155
BssT1I	CCWWGG	5 cut(s)	535, 608, 1023, 1266, 1350
Bst2UI	CCWGG	3 cut(s)	531, 582, 753
Bst4CI	ACNGT	7 cut(s)	500, 1134, 1396, 1676, 1807, 1863, 1915
Bst6I	CTCTTC	5 cut(s)	912, 1638, 1652, 2011, 2095
BstACI	GRCGYC	1 cut(s)	2155
BstAFI	CTTAAG	1 cut(s)	1460
BstAPI	GCANNNNNTGC	1 cut(s)	881
BstBAI	YACGTR	2 cut(s)	561, 2168
BstC8I	GCNNGC	4 cut(s)	392, 1767, 1848, 1995
BstDEI	CTNAG	7 cut(s)	162, 576, 1079, 1470, 1632, 1683, 1971
BstDSI	CCRYGG	4 cut(s)	608, 1023, 1266, 1350
BstF5I	GGATG	9 cut(s)	19, 93, 349, 685, 1548, 1690, 1712, 1933, 2085
BstHHI	GCGC	1 cut(s)	390
BstMAI	GTCTC	3 cut(s)	101, 1312, 1753
BstMWI	GCNNNNNNNGC	4 cut(s)	881, 911, 1166, 1604
BstNI	CCWGG	3 cut(s)	531, 582, 753
BstSCI	CCNGG	5 cut(s)	72, 529, 580, 751, 1181
BstSFI	CTRYAG	6 cut(s)	31, 1164, 1197, 1487, 1724, 1824
BstSNI	TACGTA	1 cut(s)	2168
BstV2I	GAAGAC	3 cut(s)	121, 299, 1025
BstX2I	RGATCY	4 cut(s)	1046, 1111, 1483, 1937
BstXI	CCANNNNNNTGG	1 cut(s)	1273
BstYI	RGATCY	4 cut(s)	1046, 1111, 1483, 1937
BsuI	GTATCC	1 cut(s)	1927
BsuRI	GGCC	4 cut(s)	305, 318, 534, 1022
BtgI	CCRYGG	4 cut(s)	608, 1023, 1266, 1350
BtsCI	GGATG	9 cut(s)	19, 93, 349, 685, 1548, 1690, 1712, 1933, 2085
BtsI	GCAGTG	2 cut(s)	1614, 1721
BtsIMutI	CAGTG	3 cut(s)	1614, 1721, 1803
Cac8I	GCNNGC	4 cut(s)	392, 1767, 1848, 1995
CaiI	CAGNNNCTG	5 cut(s)	833, 953, 1163, 1217, 1709
CciI	TCATGA	1 cut(s)	1342
CfoI	GCGC	1 cut(s)	390
Cfr10I	RCCGGY	1 cut(s)	331
Cfr13I	GGNCC	3 cut(s)	187, 724, 896
CseI	GACGC	1 cut(s)	2163
Csp6I	GTAC	3 cut(s)	28, 551, 1663
CspAI	ACCGGT	1 cut(s)	331
CviQI	GTAC	3 cut(s)	28, 551, 1663
DdeI	CTNAG	7 cut(s)	162, 576, 1079, 1470, 1632, 1683, 1971
EaeI	YGGCCR	1 cut(s)	303
Eam1104I	CTCTTC	5 cut(s)	912, 1638, 1652, 2011, 2095
EarI	CTCTTC	5 cut(s)	912, 1638, 1652, 2011, 2095
EciI	GGCGGA	1 cut(s)	220
Ecl136II	GAGCTC	2 cut(s)	2065, 2105
Eco105I	TACGTA	1 cut(s)	2168
Eco130I	CCWWGG	5 cut(s)	535, 608, 1023, 1266, 1350
Eco147I	AGGCCT	1 cut(s)	534
Eco24I	GRGCYC	3 cut(s)	403, 2067, 2107
Eco31I	GGTCTC	2 cut(s)	101, 1312
Eco32I	GATATC	2 cut(s)	429, 1090
Eco47I	GGWCC	3 cut(s)	187, 724, 896
Eco53kI	GAGCTC	2 cut(s)	2065, 2105
Eco57I	CTGAAG	4 cut(s)	930, 936, 1676, 1914
Eco72I	CACGTG	1 cut(s)	561
Eco88I	CYCGRG	1 cut(s)	1239
EcoICRI	GAGCTC	2 cut(s)	2065, 2105
EcoO109I	RGGNCCY	2 cut(s)	187, 896
EcoRII	CCWGG	3 cut(s)	529, 580, 751
EcoRV	GATATC	2 cut(s)	429, 1090
EcoT14I	CCWWGG	5 cut(s)	535, 608, 1023, 1266, 1350
EcoT22I	ATGCAT	1 cut(s)	1594
EcoT38I	GRGCYC	3 cut(s)	403, 2067, 2107
ErhI	CCWWGG	5 cut(s)	535, 608, 1023, 1266, 1350
FalI	AAGNNNNNCTT	2 cut(s)	505, 537
FaqI	GGGAC	2 cut(s)	882, 1849
FauI	CCCGC	3 cut(s)	223, 244, 907
FauNDI	CATATG	1 cut(s)	971
FbaI	TGATCA	2 cut(s)	643, 1221
FblI	GTMKAC	2 cut(s)	177, 526
FokI	GGATG	9 cut(s)	26, 80, 356, 692, 1555, 1677, 1699, 1940, 2092
FriOI	GRGCYC	3 cut(s)	403, 2067, 2107
FspBI	CTAG	2 cut(s)	1050, 1506
FspI	TGCGCA	1 cut(s)	389
GlaI	GCGC	1 cut(s)	389
GsaI	CCCAGC	2 cut(s)	55, 1602
GsuI	CTGGAG	4 cut(s)	349, 603, 711, 1800
HaeIII	GGCC	4 cut(s)	305, 318, 534, 1022
HapII	CCGG	8 cut(s)	74, 270, 306, 332, 338, 674, 1182, 1322
HgaI	GACGC	1 cut(s)	2163
HhaI	GCGC	1 cut(s)	390
Hin1I	GRCGYC	1 cut(s)	2155
Hin6I	GCGC	1 cut(s)	388
HinP1I	GCGC	1 cut(s)	388
HincII	GTYRAC	3 cut(s)	178, 360, 527
HindII	GTYRAC	3 cut(s)	178, 360, 527
HindIII	AAGCTT	2 cut(s)	392, 795
HinfI	GANTC	9 cut(s)	46, 203, 456, 670, 864, 1706, 1841, 2000, 2087
HpaII	CCGG	8 cut(s)	74, 270, 306, 332, 338, 674, 1182, 1322
HphI	GGTGA	2 cut(s)	1097, 1625
Hpy166II	GTNNAC	6 cut(s)	28, 178, 360, 527, 599, 724
Hpy188I	TCNGA	9 cut(s)	98, 208, 351, 741, 854, 949, 1226, 1705, 2044
Hpy8I	GTNNAC	6 cut(s)	28, 178, 360, 527, 599, 724
Hpy99I	CGWCG	3 cut(s)	70, 176, 179
HpyAV	CCTTC	6 cut(s)	11, 292, 308, 1012, 1250, 1676
HpyCH4III	ACNGT	7 cut(s)	500, 1134, 1396, 1676, 1807, 1863, 1915
HpyCH4IV	ACGT	4 cut(s)	560, 1447, 1887, 2167
HpyF10VI	GCNNNNNNNGC	4 cut(s)	881, 911, 1166, 1604
HpyF3I	CTNAG	7 cut(s)	162, 576, 1079, 1470, 1632, 1683, 1971
HpySE526I	ACGT	4 cut(s)	560, 1447, 1887, 2167
Hsp92I	GRCGYC	1 cut(s)	2155
HspAI	GCGC	1 cut(s)	388
Kpn2I	TCCGGA	1 cut(s)	673
KpnI	GGTACC	1 cut(s)	1666
Ksp22I	TGATCA	2 cut(s)	643, 1221
LguI	GCTCTTC	1 cut(s)	2095
LmnI	GCTCC	7 cut(s)	20, 228, 233, 824, 1165, 1262, 1354
LweI	GCATC	5 cut(s)	458, 871, 1601, 1721, 1918
MaeI	CTAG	2 cut(s)	1050, 1506
MaeII	ACGT	4 cut(s)	560, 1447, 1887, 2167
MaeIII	GTNAC	3 cut(s)	563, 1001, 1888
MbiI	CCGCTC	2 cut(s)	23, 228
MfeI	CAATTG	3 cut(s)	320, 546, 744
MflI	RGATCY	4 cut(s)	1046, 1111, 1483, 1937
MhlI	GDGCHC	3 cut(s)	403, 2067, 2107
MlyI	GAGTC	5 cut(s)	55, 197, 465, 1715, 1850
Mph1103I	ATGCAT	1 cut(s)	1594
MroI	TCCGGA	1 cut(s)	673
MroXI	GAANNNNTTC	1 cut(s)	128
MseI	TTAA	8 cut(s)	656, 764, 933, 1401, 1461, 1530, 1989, 2010
MslI	CAYNNNNRTG	3 cut(s)	1008, 1541, 2072
MspA1I	CMGCKG	4 cut(s)	902, 914, 1202, 1427
MspCI	CTTAAG	1 cut(s)	1460
MspI	CCGG	8 cut(s)	74, 270, 306, 332, 338, 674, 1182, 1322
MspR9I	CCNGG	5 cut(s)	74, 531, 582, 753, 1183
MunI	CAATTG	3 cut(s)	320, 546, 744
MvaI	CCWGG	3 cut(s)	531, 582, 753
MwoI	GCNNNNNNNGC	4 cut(s)	881, 911, 1166, 1604
NciI	CCSGG	2 cut(s)	74, 1183
NcoI	CCATGG	4 cut(s)	608, 1023, 1266, 1350
NdeI	CATATG	1 cut(s)	971
NlaIV	GGNNCC	9 cut(s)	330, 336, 519, 621, 898, 1113, 1264, 1664, 2033
NmeAIII	GCCGAG	1 cut(s)	171
NmuCI	GTSAC	2 cut(s)	1001, 1888
NsbI	TGCGCA	1 cut(s)	389
NsiI	ATGCAT	1 cut(s)	1594
PaeR7I	CTCGAG	1 cut(s)	1239
PagI	TCATGA	1 cut(s)	1342
PceI	AGGCCT	1 cut(s)	534
PciSI	GCTCTTC	1 cut(s)	2095
PcsI	WCGNNNNNNNCGW	1 cut(s)	198
PdmI	GAANNNNTTC	1 cut(s)	128
PfeI	GAWTC	4 cut(s)	670, 864, 2000, 2087
PflFI	GACNNNGTC	1 cut(s)	112
PflMI	CCANNNNNTGG	1 cut(s)	536
PfoI	TCCNGGA	1 cut(s)	1181
PinAI	ACCGGT	1 cut(s)	331
PleI	GAGTC	5 cut(s)	54, 197, 464, 1714, 1849
PmaCI	CACGTG	1 cut(s)	561
PmlI	CACGTG	1 cut(s)	561
PpsI	GAGTC	5 cut(s)	54, 197, 464, 1714, 1849
Ppu21I	YACGTR	2 cut(s)	561, 2168
PpuMI	RGGWCCY	2 cut(s)	187, 896
PsiI	TTATAA	1 cut(s)	2111
Psp124BI	GAGCTC	2 cut(s)	2067, 2107
Psp5II	RGGWCCY	2 cut(s)	187, 896
Psp6I	CCWGG	3 cut(s)	529, 580, 751
PspCI	CACGTG	1 cut(s)	561
PspFI	CCCAGC	2 cut(s)	51, 1598
PspGI	CCWGG	3 cut(s)	529, 580, 751
PspN4I	GGNNCC	9 cut(s)	330, 336, 519, 621, 898, 1113, 1264, 1664, 2033
PspPI	GGNCC	3 cut(s)	187, 724, 896
PspPPI	RGGWCCY	2 cut(s)	187, 896
PsrI	GAACNNNNNNTAC	2 cut(s)	1569, 1601
PstI	CTGCAG	3 cut(s)	1201, 1491, 1728
PstNI	CAGNNNCTG	5 cut(s)	833, 953, 1163, 1217, 1709
PsuI	RGATCY	4 cut(s)	1046, 1111, 1483, 1937
PsyI	GACNNNGTC	1 cut(s)	112
PvuII	CAGCTG	3 cut(s)	914, 1202, 1427
RsaI	GTAC	3 cut(s)	29, 552, 1664
RsaNI	GTAC	3 cut(s)	28, 551, 1663
RseI	CAYNNNNRTG	3 cut(s)	1008, 1541, 2072
SacI	GAGCTC	2 cut(s)	2067, 2107
SalI	GTCGAC	2 cut(s)	176, 525
SapI	GCTCTTC	1 cut(s)	2095
SaqAI	TTAA	8 cut(s)	656, 764, 933, 1401, 1461, 1530, 1989, 2010
Sau96I	GGNCC	3 cut(s)	187, 724, 896
SchI	GAGTC	5 cut(s)	55, 197, 465, 1715, 1850
ScrFI	CCNGG	5 cut(s)	74, 531, 582, 753, 1183
SduI	GDGCHC	3 cut(s)	403, 2067, 2107
SfaNI	GCATC	5 cut(s)	458, 871, 1601, 1721, 1918
SfcI	CTRYAG	6 cut(s)	31, 1164, 1197, 1487, 1724, 1824
Sfr274I	CTCGAG	1 cut(s)	1239
SinI	GGWCC	3 cut(s)	187, 724, 896
SlaI	CTCGAG	1 cut(s)	1239
SmiMI	CAYNNNNRTG	3 cut(s)	1008, 1541, 2072
SmlI	CTYRAG	4 cut(s)	889, 1139, 1239, 1460
SmoI	CTYRAG	4 cut(s)	889, 1139, 1239, 1460
SnaBI	TACGTA	1 cut(s)	2168
SseBI	AGGCCT	1 cut(s)	534
SsiI	CCGC	9 cut(s)	23, 56, 216, 226, 231, 237, 267, 727, 900
SspI	AATATT	1 cut(s)	1963
SspMI	CTAG	2 cut(s)	1050, 1506
SstI	GAGCTC	2 cut(s)	2067, 2107
StuI	AGGCCT	1 cut(s)	534
StyD4I	CCNGG	5 cut(s)	72, 529, 580, 751, 1181
StyI	CCWWGG	5 cut(s)	535, 608, 1023, 1266, 1350
TaaI	ACNGT	7 cut(s)	500, 1134, 1396, 1676, 1807, 1863, 1915
TaiI	ACGT	4 cut(s)	563, 1450, 1890, 2170
TaqI	TCGA	8 cut(s)	105, 177, 201, 526, 867, 1240, 1678, 2136
TatI	WGTACW	1 cut(s)	27
TauI	GCSGC	2 cut(s)	59, 269
TfiI	GAWTC	4 cut(s)	670, 864, 2000, 2087
Tru1I	TTAA	8 cut(s)	656, 764, 933, 1401, 1461, 1530, 1989, 2010
Tru9I	TTAA	8 cut(s)	656, 764, 933, 1401, 1461, 1530, 1989, 2010
TscAI	CASTG	3 cut(s)	1614, 1728, 1810
TseFI	GTSAC	2 cut(s)	1001, 1888
Tsp45I	GTSAC	2 cut(s)	1001, 1888
TspRI	CASTG	3 cut(s)	1614, 1728, 1810
Tth111I	GACNNNGTC	1 cut(s)	112
Van91I	CCANNNNNTGG	1 cut(s)	536
Vha464I	CTTAAG	1 cut(s)	1460
VpaK11BI	GGWCC	3 cut(s)	187, 724, 896
XapI	RAATTY	6 cut(s)	80, 286, 777, 929, 938, 1944
XbaI	TCTAGA	2 cut(s)	1049, 1505
XcmI	CCANNNNNNNNNTGG	1 cut(s)	606
XhoI	CTCGAG	1 cut(s)	1239
XmiI	GTMKAC	2 cut(s)	177, 526
XmnI	GAANNNNTTC	1 cut(s)	128
XspI	CTAG	2 cut(s)	1050, 1506
Zsp2I	ATGCAT	1 cut(s)	1594

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.