Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0015953

Physical Location & Seq

Genomic Coordinates Reverse (-)

11678865 .. 11683738

Gene Structure 4874 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1157500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2202 bp

ATGTCAGGGTGGGACGAAGGAGCGATCTATTACAGCGACCAAGCGCAGTCTCTCGGCGGCGATGGAGGAGACCCAGGAAGCAACGCCGCAGCTGCCACCCGCCACTCGGTCCTCCAGAAATTCAAGGAATTCATCCGAAGCTTTGAGACCCAGAAAAATGTGTTCTCCTACAGGGAGAGCCTCCTCCACAACCCTAAGTACCTTCTCGTCGACATTGAAGACCTCGATTCTTTCGACGCCGACCTCGTCGCAAAGCTCCGCTCTGCCCCCTCCGATTACTTGCCTCTGTTTGAGAACGCCGCCGGTCAGGTTTTGGCGAATTTGAAGACGAAAGTTCCCGGAAACGAAGGGAAATTGGAGGAACGGGTGCCGGAGGATGTTCAGATCTTGCTGACCTCCAAAGAGGACCCGGCGTCCATGCGCCAGCTCGGGGCACAATGCATATCAAAACTCATCAAGGTTTCCGGGATCACTATAGCTGCTTCGAGGGTCAAGGCGAAGGCGACTTATGTGATTGTCATGTGTAAGAACTGTAAAAATGTGCAGAGGGTACCTTGCCGGCCAGGTCTTGGTGGGGCAATCGTCCCTCGCTCTTGCAACCATATTCCTCAGCCTGGAGAAGAGCCTTGTCCGCTTGATCCGTGGGTTGTGGTTCCTGATAAGAGCAAGTATGTTGATCAGCAAACCTTGAAATTGCAAGAGAATCCAGAGGATGTACCTACCGGAGAGCTTCCAAGAAATATGCTTCTATCTGTGGACCGTCATCTTGTTCAGACAATTGTGCCTGGCACAAGATTAACCATCATGGGCATTTATAGCATCTATCAAGCTTCCAATTCTGCCACATCTCACAAGGGAGCAGTTGCTGTTAGGCAGCCTTACATCAGAGTGGTAGGAATAGAAGAAGCAACTGATGCCAACTCTCGTGGTCCCTCTGCTTTTACACAAGACGAGATAGAAGAGTTCAAGAGGTTTGCTTCAGAAGATGCTTATAAAAATATATGCTCGAAAATTGCTCCCTCTATATTTGGTCATGAAGATGTAAAGAAGGCTGTGGCGTGTCTTCTGTTCGGGGGATCTAGAAAGCATTTGCCTGATGGTGTGAAGCTAAGAGGTGATATTAATGTCTTACTTTTGGGGGATCCATCTACTGCTAAATCACAGTTTCTCAAGTTTGTCGAGAAGACAGCTCCGGTGGCCGTATATACTTCAGGAAAAGGGTCATCAGCTGCTGGTCTTACAGCTTCTGTTATCAGGGATAGCAGTTCTCGTGAGTTTTATCTTGAAGGAGGAGCCATGGTTTTAGCGGATGGAGGTGTTGTCTGTATTGACGAGTTTGACAAAATGAGATCAGAGGATAGGGTTGCTATTCATGAAGCCATGGAGCAGCAAACCATATCCATTGCCAAAGCAGGAATAACAACCGTTCTTAATTCTAGAACCTCTGTGCTTGCAGCTGCTAACCCTCCATCTGGACGTTATGACGATCTTAAGACTGCTCAGGATAATATCGATCTGCAGACGACAATTCTTTCGAGATTTGACTTGATATTCATTGTTAAAGATATCAGGATGTACAGTCAGGATAAGATTATAGCAAGTCATATCATAAAGGTTCATGCGTCTGCTGGTGCAGCACTGGGTGAAGGTAGAGTAGTTTCTAAAGAAGAGAATTGGCTGAAGAGGTACATACAATATTGTCGAACTGAATGCCACCCCCGTTTATCAGAAACTGCGTCGAGAGAGCTGCAGAGTAATTATGTGAAAATCAGACAGGACATGAGACAGCTAGCAAATGAAACTGGAGAGGCTGCTGCAGTCCCAATCACTGTAAGGCAGCTTGAAGCTATAGTGAGGTTGAGTGAGGCCCTTGCAAAAATGCAACTGCGCCACGAAGCTACTGAGGAGCATGTTCGTGAAGCAATAAGACTATTCAACGCAGCCACAATGGACGCGGCAAAGTCTGGAATACAGCAACAAGTCAATCTCACTGGTGAGATGGCGAATGAAGTTAAGCAAGCTGAAACTCAGATAAGGAGAAGAGTAGGCATTGGGAACCACATATCAGAGAGAAAGCTGATCGATGAGCTCACAAGAATGGGGATGAATGAATCCATTGTGAGAAGAGCTCTTATTATTATGCACCAGAGGGATGAAGTCGAATACAAGCGAGAAAGACGCGTTATTTTACGCAAAGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 ko05206 map03030 map04110 map04111 map04113 map05206

Pfam Domains

Protein Families

MCM MCM_N MCM_OB Mg_chelatase

Protein Analysis

734

Amino Acids

80.99

Weight (kDa)

8.13

Isoelectric Point (pI)

47.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	39 - 125	7.9e-13	MCM N-terminal domain
MCM_OB	PF17207	140 - 275	2.7e-34	MCM OB domain
MCM	PF00493	319 - 540	1.3e-100	MCM P-loop domain
MCM_lid	PF17855	559 - 648	1.1e-28	MCM AAA-lid domain
MCM5_C	PF21933	673 - 731	8.1e-18	MCM5, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015953)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G07690 AT2G07690
fragaria_vesca	FvH4_1g01490
malus_domestica	MD02G1011900.v1.1 MD15G1157500.v1.1
prunus_persica	Prupe.7G252700_v2.0.a1
pyrus_communis	pycom15g14130
rosa_chinensis	RchiOBHm_Chr2g0086411
rosa_laevigata	RLG00000015756
rosa_multiflora	Rmu_sc0005127.1_g000006
rosa_roxburghii	Rroxscaffold_2G00154600
rosa_rugosa	Rorug01G0467200
rosa_samantha	Rh2AG017800 Rh2DG019100
rosa_wichuraiana	Rw2G001480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	993
Acc65I	GGTACC	1 cut(s)	550
AccB1I	GGYRCC	2 cut(s)	367, 550
AccB7I	CCANNNNNTGG	1 cut(s)	569
AccBSI	CCGCTC	1 cut(s)	261
AccI	GTMKAC	1 cut(s)	210
AccII	CGCG	2 cut(s)	1955, 2181
AciI	CCGC	8 cut(s)	57, 87, 100, 259, 300, 632, 1307, 1955
AclWI	GGATC	5 cut(s)	476, 632, 1084, 1136, 1149
AcoI	YGGCCR	2 cut(s)	560, 1197
AcsI	RAATTY	3 cut(s)	119, 128, 319
AcuI	CTGAAG	3 cut(s)	963, 1194, 1700
AcyI	GRCGYC	2 cut(s)	237, 413
AdeI	CACNNNGTG	1 cut(s)	1643
AfaI	GTAC	5 cut(s)	200, 552, 717, 1577, 1688
AfiI	CCNNNNNNNGG	5 cut(s)	106, 430, 569, 614, 1472
AflII	CTTAAG	1 cut(s)	1490
AflIII	ACRYGT	1 cut(s)	2179
AgsI	TTSAA	8 cut(s)	124, 218, 325, 691, 967, 1286, 1844, 1936
AhdI	GACNNNNNGTC	1 cut(s)	412
AjnI	CCWGG	4 cut(s)	73, 562, 613, 784
Alw21I	GWGCWC	2 cut(s)	2091, 2131
Alw26I	GTCTC	4 cut(s)	54, 63, 140, 1777
AlwI	GGATC	5 cut(s)	476, 632, 1084, 1136, 1149
AlwNI	CAGNNNCTG	4 cut(s)	866, 1232, 1247, 1733
Ama87I	CYCGRG	1 cut(s)	428
AoxI	GGCC	3 cut(s)	560, 1197, 1866
ApoI	RAATTY	3 cut(s)	119, 128, 319
ArsI	GACNNNNNNTTYG	2 cut(s)	1516, 1548
AseI	ATTAAT	1 cut(s)	1122
Asp718I	GGTACC	1 cut(s)	550
AspLEI	GCGC	3 cut(s)	46, 423, 1890
AspS9I	GGNCC	5 cut(s)	109, 406, 757, 929, 1867
AsuC2I	CCSGG	3 cut(s)	339, 410, 466
AsuHPI	GGTGA	3 cut(s)	1127, 1655, 2006
AsuNHI	GCTAGC	1 cut(s)	1789
AvaI	CYCGRG	1 cut(s)	428
AvaII	GGWCC	4 cut(s)	109, 406, 757, 929
BaeGI	GKGCMC	1 cut(s)	436
BamHI	GGATCC	1 cut(s)	1141
BanI	GGYRCC	2 cut(s)	367, 550
BanII	GRGCYC	2 cut(s)	2091, 2131
BauI	CACGAG	2 cut(s)	924, 1269
BbsI	GAAGAC	4 cut(s)	225, 332, 1055, 1190
Bbv12I	GWGCWC	2 cut(s)	2091, 2131
BbvCI	CCTCAGC	1 cut(s)	609
BccI	CCATC	7 cut(s)	56, 809, 1091, 1153, 1304, 1477, 1993
BceAI	ACGGC	1 cut(s)	1184
BcgI	CGANNNNNNTGC	4 cut(s)	1692, 1726, 1729, 1763
BciT130I	CCWGG	4 cut(s)	75, 564, 615, 786
BclI	TGATCA	1 cut(s)	676
BcnI	CCSGG	3 cut(s)	339, 410, 466
BcoDI	GTCTC	4 cut(s)	54, 63, 140, 1777
BfaI	CTAG	3 cut(s)	1080, 1437, 1790
BfmI	CTRYAG	6 cut(s)	169, 474, 1517, 1748, 1815, 1848
BfrI	CTTAAG	1 cut(s)	1490
BglII	AGATCT	1 cut(s)	384
Bme1390I	CCNGG	7 cut(s)	75, 339, 410, 466, 564, 615, 786
Bme18I	GGWCC	4 cut(s)	109, 406, 757, 929
BmeRI	GACNNNNNGTC	1 cut(s)	412
BmeT110I	CYCGRG	1 cut(s)	428
BmgT120I	GGNCC	5 cut(s)	109, 406, 757, 929, 1867
BmiI	GGNNCC	8 cut(s)	369, 408, 552, 654, 931, 1143, 1294, 2057
BmrFI	CCNGG	7 cut(s)	75, 339, 410, 466, 564, 615, 786
BmrI	ACTGGG	1 cut(s)	1649
BmsI	GCATC	3 cut(s)	828, 904, 976
BmtI	GCTAGC	1 cut(s)	1793
BmuI	ACTGGG	1 cut(s)	1649
BpiI	GAAGAC	4 cut(s)	225, 332, 1055, 1190
BplI	GAGNNNNNCTC	2 cut(s)	2113, 2145
BpmI	CTGGAG	3 cut(s)	98, 636, 1824
Bpu10I	CCTNAGC	2 cut(s)	609, 1500
BpuEI	CTTGAG	1 cut(s)	1154
BpuMI	CCSGG	3 cut(s)	339, 410, 466
Bsa29I	ATCGAT	2 cut(s)	1512, 2082
BsaHI	GRCGYC	2 cut(s)	237, 413
BsaI	GGTCTC	2 cut(s)	63, 140
BsaJI	CCNNGG	4 cut(s)	73, 641, 1296, 1380
BsaWI	WCCGGW	2 cut(s)	722, 1192
BsaXI	ACNNNNNCTCC	4 cut(s)	1284, 1305, 1314, 1335
Bsc4I	CCNNNNNNNGG	5 cut(s)	106, 430, 569, 614, 1472
Bse118I	RCCGGY	2 cut(s)	302, 558
Bse1I	ACTGG	3 cut(s)	1644, 1807, 1996
Bse3DI	GCAATG	1 cut(s)	1401
BseBI	CCWGG	4 cut(s)	75, 564, 615, 786
BseCI	ATCGAT	2 cut(s)	1512, 2082
BseDI	CCNNGG	4 cut(s)	73, 641, 1296, 1380
BseGI	GGATG	7 cut(s)	132, 382, 718, 1315, 1578, 2109, 2158
BseLI	CCNNNNNNNGG	5 cut(s)	106, 430, 569, 614, 1472
BseMI	GCAATG	1 cut(s)	1401
BseMII	CTCAG	4 cut(s)	623, 1514, 1893, 2042
BseNI	ACTGG	3 cut(s)	1644, 1807, 1996
BseRI	GAGGAG	4 cut(s)	81, 173, 1305, 1919
BseSI	GKGCMC	1 cut(s)	436
BsgI	GTGCAG	2 cut(s)	563, 1653
Bsh1236I	CGCG	2 cut(s)	1955, 2181
BshFI	GGCC	3 cut(s)	562, 1199, 1868
BshNI	GGYRCC	2 cut(s)	367, 550
BshVI	ATCGAT	2 cut(s)	1512, 2082
BsiHKAI	GWGCWC	2 cut(s)	2091, 2131
BsiHKCI	CYCGRG	1 cut(s)	428
BsiSI	CCGG	8 cut(s)	303, 339, 371, 410, 465, 559, 723, 1193
BslFI	GGGAC	4 cut(s)	26, 569, 915, 1805
BslI	CCNNNNNNNGG	5 cut(s)	106, 430, 569, 614, 1472
BsmAI	GTCTC	4 cut(s)	54, 63, 140, 1777
BsmFI	GGGAC	4 cut(s)	26, 569, 915, 1805
BsmI	GAATGC	1 cut(s)	1715
BsnI	GGCC	3 cut(s)	562, 1199, 1868
Bso31I	GGTCTC	2 cut(s)	63, 140
BsoBI	CYCGRG	1 cut(s)	428
Bsp1286I	GDGCHC	3 cut(s)	436, 2091, 2131
Bsp1407I	TGTACA	1 cut(s)	1575
Bsp19I	CCATGG	2 cut(s)	1296, 1380
BspACI	CCGC	8 cut(s)	57, 87, 100, 259, 300, 632, 1307, 1955
BspANI	GGCC	3 cut(s)	562, 1199, 1868
BspCNI	CTCAG	4 cut(s)	622, 1513, 1894, 2041
BspDI	ATCGAT	2 cut(s)	1512, 2082
BspFNI	CGCG	2 cut(s)	1955, 2181
BspHI	TCATGA	2 cut(s)	1033, 1372
BspLI	GGNNCC	8 cut(s)	369, 408, 552, 654, 931, 1143, 1294, 2057
BspMAI	CTGCAG	3 cut(s)	1521, 1752, 1819
BspOI	GCTAGC	1 cut(s)	1793
BspPI	GGATC	5 cut(s)	476, 632, 1084, 1136, 1149
BspQI	GCTCTTC	2 cut(s)	615, 2119
BspT107I	GGYRCC	2 cut(s)	367, 550
BspTI	CTTAAG	1 cut(s)	1490
BspTNI	GGTCTC	2 cut(s)	63, 140
BsrBI	CCGCTC	1 cut(s)	261
BsrDI	GCAATG	1 cut(s)	1401
BsrFI	RCCGGY	2 cut(s)	302, 558
BsrGI	TGTACA	1 cut(s)	1575
BsrI	ACTGG	3 cut(s)	1644, 1807, 1996
BssAI	RCCGGY	2 cut(s)	302, 558
BssECI	CCNNGG	4 cut(s)	73, 641, 1296, 1380
BssNI	GRCGYC	2 cut(s)	237, 413
BssSI	CACGAG	2 cut(s)	924, 1269
BssT1I	CCWWGG	2 cut(s)	1296, 1380
Bst2BI	CACGAG	2 cut(s)	924, 1269
Bst2UI	CCWGG	4 cut(s)	75, 564, 615, 786
Bst4CI	ACNGT	6 cut(s)	533, 761, 1164, 1426, 1580, 1831
Bst6I	CTCTTC	6 cut(s)	615, 954, 1662, 1676, 2035, 2119
BstACI	GRCGYC	2 cut(s)	237, 413
BstAFI	CTTAAG	1 cut(s)	1490
BstAPI	GCANNNNNTGC	1 cut(s)	914
BstAUI	TGTACA	1 cut(s)	1575
BstC8I	GCNNGC	5 cut(s)	425, 560, 1452, 1791, 2019
BstDEI	CTNAG	6 cut(s)	195, 609, 1109, 1500, 1902, 2028
BstDSI	CCRYGG	3 cut(s)	641, 1296, 1380
BstF5I	GGATG	7 cut(s)	132, 382, 718, 1315, 1578, 2109, 2158
BstFNI	CGCG	2 cut(s)	1955, 2181
BstHHI	GCGC	3 cut(s)	46, 423, 1890
BstMAI	GTCTC	4 cut(s)	54, 63, 140, 1777
BstMWI	GCNNNNNNNGC	6 cut(s)	92, 631, 816, 914, 1196, 1634
BstNI	CCWGG	4 cut(s)	75, 564, 615, 786
BstNSI	RCATGY	1 cut(s)	1913
BstSCI	CCNGG	7 cut(s)	73, 337, 408, 464, 562, 613, 784
BstSFI	CTRYAG	6 cut(s)	169, 474, 1517, 1748, 1815, 1848
BstSLI	GKGCMC	1 cut(s)	436
BstUI	CGCG	2 cut(s)	1955, 2181
BstV2I	GAAGAC	4 cut(s)	225, 332, 1055, 1190
BstX2I	RGATCY	3 cut(s)	384, 1076, 1141
BstYI	RGATCY	3 cut(s)	384, 1076, 1141
Bsu15I	ATCGAT	2 cut(s)	1512, 2082
BsuRI	GGCC	3 cut(s)	562, 1199, 1868
BsuTUI	ATCGAT	2 cut(s)	1512, 2082
BtgI	CCRYGG	3 cut(s)	641, 1296, 1380
BtgZI	GCGATG	1 cut(s)	75
BtsCI	GGATG	7 cut(s)	132, 382, 718, 1315, 1578, 2109, 2158
BtsIMutI	CAGTG	3 cut(s)	1637, 1827, 1989
Cac8I	GCNNGC	5 cut(s)	425, 560, 1452, 1791, 2019
CaiI	CAGNNNCTG	4 cut(s)	866, 1232, 1247, 1733
CciI	TCATGA	2 cut(s)	1033, 1372
CfoI	GCGC	3 cut(s)	46, 423, 1890
Cfr10I	RCCGGY	2 cut(s)	302, 558
Cfr13I	GGNCC	5 cut(s)	109, 406, 757, 929, 1867
ClaI	ATCGAT	2 cut(s)	1512, 2082
CseI	GACGC	6 cut(s)	245, 402, 1611, 1725, 1961, 2187
Csp6I	GTAC	5 cut(s)	199, 551, 716, 1576, 1687
CspCI	CAANNNNNGTGG	2 cut(s)	907, 942
CviQI	GTAC	5 cut(s)	199, 551, 716, 1576, 1687
DdeI	CTNAG	6 cut(s)	195, 609, 1109, 1500, 1902, 2028
DraIII	CACNNNGTG	1 cut(s)	1643
DriI	GACNNNNNGTC	1 cut(s)	412
EaeI	YGGCCR	2 cut(s)	560, 1197
Eam1104I	CTCTTC	6 cut(s)	615, 954, 1662, 1676, 2035, 2119
Eam1105I	GACNNNNNGTC	1 cut(s)	412
EarI	CTCTTC	6 cut(s)	615, 954, 1662, 1676, 2035, 2119
Ecl136II	GAGCTC	2 cut(s)	2089, 2129
Eco130I	CCWWGG	2 cut(s)	1296, 1380
Eco24I	GRGCYC	2 cut(s)	2091, 2131
Eco31I	GGTCTC	2 cut(s)	63, 140
Eco32I	GATATC	1 cut(s)	1567
Eco47I	GGWCC	4 cut(s)	109, 406, 757, 929
Eco53kI	GAGCTC	2 cut(s)	2089, 2129
Eco57I	CTGAAG	3 cut(s)	963, 1194, 1700
Eco88I	CYCGRG	1 cut(s)	428
EcoICRI	GAGCTC	2 cut(s)	2089, 2129
EcoO109I	RGGNCCY	2 cut(s)	406, 1867
EcoRI	GAATTC	1 cut(s)	128
EcoRII	CCWGG	4 cut(s)	73, 562, 613, 784
EcoRV	GATATC	1 cut(s)	1567
EcoT14I	CCWWGG	2 cut(s)	1296, 1380
EcoT22I	ATGCAT	1 cut(s)	443
EcoT38I	GRGCYC	2 cut(s)	2091, 2131
ErhI	CCWWGG	2 cut(s)	1296, 1380
FaqI	GGGAC	4 cut(s)	26, 569, 915, 1805
FauI	CCCGC	1 cut(s)	107
FbaI	TGATCA	1 cut(s)	676
FblI	GTMKAC	1 cut(s)	210
FokI	GGATG	7 cut(s)	119, 389, 725, 1322, 1585, 2116, 2165
FriOI	GRGCYC	2 cut(s)	2091, 2131
FspBI	CTAG	3 cut(s)	1080, 1437, 1790
GlaI	GCGC	3 cut(s)	45, 422, 1889
GsuI	CTGGAG	3 cut(s)	98, 636, 1824
HaeIII	GGCC	3 cut(s)	562, 1199, 1868
HapII	CCGG	8 cut(s)	303, 339, 371, 410, 465, 559, 723, 1193
HgaI	GACGC	6 cut(s)	245, 402, 1611, 1725, 1961, 2187
HhaI	GCGC	3 cut(s)	46, 423, 1890
Hin1I	GRCGYC	2 cut(s)	237, 413
Hin6I	GCGC	3 cut(s)	44, 421, 1888
HinP1I	GCGC	3 cut(s)	44, 421, 1888
HincII	GTYRAC	1 cut(s)	211
HindII	GTYRAC	1 cut(s)	211
HindIII	AAGCTT	2 cut(s)	139, 828
HinfI	GANTC	3 cut(s)	227, 703, 2111
HpaII	CCGG	8 cut(s)	303, 339, 371, 410, 465, 559, 723, 1193
HphI	GGTGA	3 cut(s)	1127, 1655, 2006
Hpy166II	GTNNAC	2 cut(s)	211, 757
Hpy8I	GTNNAC	2 cut(s)	211, 757
Hpy99I	CGWCG	4 cut(s)	212, 239, 251, 1741
HpyAV	CCTTC	7 cut(s)	11, 212, 341, 493, 1042, 1280, 1640
HpyCH4III	ACNGT	6 cut(s)	533, 761, 1164, 1426, 1580, 1831
HpyCH4IV	ACGT	1 cut(s)	1477
HpyF10VI	GCNNNNNNNGC	6 cut(s)	92, 631, 816, 914, 1196, 1634
HpyF3I	CTNAG	6 cut(s)	195, 609, 1109, 1500, 1902, 2028
HpySE526I	ACGT	1 cut(s)	1477
Hsp92I	GRCGYC	2 cut(s)	237, 413
HspAI	GCGC	3 cut(s)	44, 421, 1888
KpnI	GGTACC	1 cut(s)	554
KroI	GCCGGC	1 cut(s)	558
KroNI	GCCGGC	1 cut(s)	560
Ksp22I	TGATCA	1 cut(s)	676
LguI	GCTCTTC	2 cut(s)	615, 2119
LmnI	GCTCC	8 cut(s)	20, 261, 857, 1021, 1195, 1292, 1384, 1906
LweI	GCATC	3 cut(s)	828, 904, 976
MaeI	CTAG	3 cut(s)	1080, 1437, 1790
MaeII	ACGT	1 cut(s)	1477
MbiI	CCGCTC	1 cut(s)	261
MfeI	CAATTG	1 cut(s)	777
MflI	RGATCY	3 cut(s)	384, 1076, 1141
MhlI	GDGCHC	3 cut(s)	436, 2091, 2131
MluI	ACGCGT	1 cut(s)	2179
Mph1103I	ATGCAT	1 cut(s)	443
MroNI	GCCGGC	1 cut(s)	558
MseI	TTAA	6 cut(s)	797, 1122, 1431, 1491, 1560, 2013
MslI	CAYNNNNRTG	4 cut(s)	887, 1038, 1914, 2096
MspA1I	CMGCKG	3 cut(s)	92, 1229, 1457
MspCI	CTTAAG	1 cut(s)	1490
MspI	CCGG	8 cut(s)	303, 339, 371, 410, 465, 559, 723, 1193
MspR9I	CCNGG	7 cut(s)	75, 339, 410, 466, 564, 615, 786
MunI	CAATTG	1 cut(s)	777
Mva1269I	GAATGC	1 cut(s)	1715
MvaI	CCWGG	4 cut(s)	75, 564, 615, 786
MvnI	CGCG	2 cut(s)	1955, 2181
MwoI	GCNNNNNNNGC	6 cut(s)	92, 631, 816, 914, 1196, 1634
NaeI	GCCGGC	1 cut(s)	560
NciI	CCSGG	3 cut(s)	339, 410, 466
NcoI	CCATGG	2 cut(s)	1296, 1380
NgoMIV	GCCGGC	1 cut(s)	558
NheI	GCTAGC	1 cut(s)	1789
NlaIV	GGNNCC	8 cut(s)	369, 408, 552, 654, 931, 1143, 1294, 2057
NmeAIII	GCCGAG	1 cut(s)	33
NsiI	ATGCAT	1 cut(s)	443
NspI	RCATGY	1 cut(s)	1913
PagI	TCATGA	2 cut(s)	1033, 1372
PciSI	GCTCTTC	2 cut(s)	615, 2119
PcsI	WCGNNNNNNNCGW	2 cut(s)	231, 243
PctI	GAATGC	1 cut(s)	1715
PdiI	GCCGGC	1 cut(s)	560
PfeI	GAWTC	3 cut(s)	227, 703, 2111
PflFI	GACNNNGTC	1 cut(s)	245
PflMI	CCANNNNNTGG	1 cut(s)	569
PfoI	TCCNGGA	2 cut(s)	337, 464
PpuMI	RGGWCCY	1 cut(s)	406
PshBI	ATTAAT	1 cut(s)	1122
PsiI	TTATAA	1 cut(s)	993
Psp124BI	GAGCTC	2 cut(s)	2091, 2131
Psp5II	RGGWCCY	1 cut(s)	406
Psp6I	CCWGG	4 cut(s)	73, 562, 613, 784
PspGI	CCWGG	4 cut(s)	73, 562, 613, 784
PspN4I	GGNNCC	8 cut(s)	369, 408, 552, 654, 931, 1143, 1294, 2057
PspPI	GGNCC	5 cut(s)	109, 406, 757, 929, 1867
PspPPI	RGGWCCY	1 cut(s)	406
PstI	CTGCAG	3 cut(s)	1521, 1752, 1819
PstNI	CAGNNNCTG	4 cut(s)	866, 1232, 1247, 1733
PsuI	RGATCY	3 cut(s)	384, 1076, 1141
PsyI	GACNNNGTC	1 cut(s)	245
PvuII	CAGCTG	3 cut(s)	92, 1229, 1457
RsaI	GTAC	5 cut(s)	200, 552, 717, 1577, 1688
RsaNI	GTAC	5 cut(s)	199, 551, 716, 1576, 1687
RseI	CAYNNNNRTG	4 cut(s)	887, 1038, 1914, 2096
SacI	GAGCTC	2 cut(s)	2091, 2131
SalI	GTCGAC	1 cut(s)	209
SapI	GCTCTTC	2 cut(s)	615, 2119
SaqAI	TTAA	6 cut(s)	797, 1122, 1431, 1491, 1560, 2013
Sau96I	GGNCC	5 cut(s)	109, 406, 757, 929, 1867
ScrFI	CCNGG	7 cut(s)	75, 339, 410, 466, 564, 615, 786
SduI	GDGCHC	3 cut(s)	436, 2091, 2131
SfaNI	GCATC	3 cut(s)	828, 904, 976
SfcI	CTRYAG	6 cut(s)	169, 474, 1517, 1748, 1815, 1848
SinI	GGWCC	4 cut(s)	109, 406, 757, 929
SmiMI	CAYNNNNRTG	4 cut(s)	887, 1038, 1914, 2096
SmlI	CTYRAG	2 cut(s)	1169, 1490
SmoI	CTYRAG	2 cut(s)	1169, 1490
SsiI	CCGC	8 cut(s)	57, 87, 100, 259, 300, 632, 1307, 1955
SspI	AATATT	1 cut(s)	1697
SspMI	CTAG	3 cut(s)	1080, 1437, 1790
SstI	GAGCTC	2 cut(s)	2091, 2131
StyD4I	CCNGG	7 cut(s)	73, 337, 408, 464, 562, 613, 784
StyI	CCWWGG	2 cut(s)	1296, 1380
TaaI	ACNGT	6 cut(s)	533, 761, 1164, 1426, 1580, 1831
TaiI	ACGT	1 cut(s)	1480
TaqII	GACCGA	1 cut(s)	97
TatI	WGTACW	1 cut(s)	1575
TauI	GCSGC	4 cut(s)	60, 89, 302, 1958
TfiI	GAWTC	3 cut(s)	227, 703, 2111
Tru1I	TTAA	6 cut(s)	797, 1122, 1431, 1491, 1560, 2013
Tru9I	TTAA	6 cut(s)	797, 1122, 1431, 1491, 1560, 2013
TscAI	CASTG	3 cut(s)	1644, 1834, 1996
TspGWI	ACGGA	1 cut(s)	630
TspRI	CASTG	3 cut(s)	1644, 1834, 1996
Tth111I	GACNNNGTC	1 cut(s)	245
Van91I	CCANNNNNTGG	1 cut(s)	569
Vha464I	CTTAAG	1 cut(s)	1490
VpaK11BI	GGWCC	4 cut(s)	109, 406, 757, 929
VspI	ATTAAT	1 cut(s)	1122
XapI	RAATTY	3 cut(s)	119, 128, 319
XbaI	TCTAGA	2 cut(s)	1079, 1436
XceI	RCATGY	1 cut(s)	1913
XmiI	GTMKAC	1 cut(s)	210
XspI	CTAG	3 cut(s)	1080, 1437, 1790
Zsp2I	ATGCAT	1 cut(s)	443

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.