Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0006469

Physical Location & Seq

Genomic Coordinates Forward (+)

2103040 .. 2109623

Gene Structure 6584 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g03960.t5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2247 bp

ATGTCATTTCCACAATTCCTTCTGAACTTGCTAGGAAGATCTCCCCTAAACCTTTCAGGTCTTATGGCTTTTTCAACATGCTCCGGCTTTGGCGTCTTAGAAGAGTTAGTGCTCTATTTTCCAGATTGGAGAAAGATAGGAACTATAACTACTTTTGGGTTCGTTGTGCAAAGCTTATTTGTTGCTGGATGCTTCTATTACCTTCTTGCAGCGCGTTACCGCGACCCCAGTAACACCTGGATTGGAAAATCAATGGAAGACTTTCTTCATCAAAGTATATGGATTCGATACGTGACTTCAGTTTATTGGTCTATCACTACACTCACAACAGTTGGCTATGGAGATTTGCATCCTGTCAATACAAGGGAGATGATCTTTGACATATTCTACATGCTTTTCAACCTTGGATTGACATCATACTTGATTGGAAATATGACCAACTTGGTTGTCCATGGGACTAGTCGAACACGAAAATTTAGGGATACCATACAAGCTGCCTCCAGTTTTGCACAGAGGAACCAACTGCCTGTTCGCCTGCAGGATCAAATGCTTGCTCATTTGTGTCTGAAGTTCAGGACAGATGCAGAGGGACTCCAGCAACAAGAGACTCTTGATTCACTTCCTAAAGCCATCCGCTCAAGTATCTCACATTATCTGTTTTACTCTCTTGTCGATAAGGTGTACTTGTTTCGTGGGGTTTCAAATGACTTGCTTTTTCAGTTGGTCTCAGAGATGAAAGCCGAGTACTTTCCTCCCAAAGAAGACATAATATTGCAGAATGAAGCACCTACAGATTTCTACATACTTGTAACTGGTGCTGCGGATTTAGTGGTACTCAAGAATGGAGCTGAACAGGTCATTGGTGAGGCAAAAACTGGTGATCTCATTGGTGAAATTGGGGTACTTTGTTATAGGCCACAGCTCTTTACAGTAAGGACCAAAAGATTGAGTCAGCTACTCCGGCTCAACAGAACCAAATTTTTAAGCATAGTGCAAGCTAATGTTGGAGATGGAACTATAATAATGAATAACCTCCTCGAGCGTTTGAAAGAGACGTCCAAGGATCCATATATGGAGGGAGTTTTGTTGGAGACTGAGAACATGCTAGCTCGTGGTAGATTGGATCTACCTCTCAGTCTATGCTTTGCAGCAATGAGAGGAGATGACTTGCTGTTGCATCAGTTGTTGAGACGGGGTTTGGATCCGAATGAATCCGACAACAATGGAAGAACAGCTCTGCATATAGCTGCATCCCAAGGAAGTGAGAACTGTGTGCTTCTACTATTGGACTATGGAGCACTTCCTAACAGTAGAGACTCGGAGGGAAATGTACCACTGTGGGAGGCGATACAGAAGGATCATGAAACGATTGCGAAATTGCTCGTAGACAATGGTGCAACTTTGAATGCTGGGGATGTTGGTCATTTCGCTTGCATTGCTACTGAGGAAAACAGGTTGGACTTGCTCAAAGAAATAGTTCGACATGGAGGTGATGTCACAACTCCTAGAGGCAATGGAACCACGGCTCTCCATGTCGCAGTGTCCGAAGACAACATTGAAATTGTGAAATTCCTTGTGGAAAAGGGGGCTGATATCGACAAGCCAGACCTCCATGGCTGGACTGCAAGAACTCTAGCTGACCAGCAAGGACATGAAGAGATAAAAAGCCTTTTTCAATCGAGAAAAGAGACCAAAGATCAATCTGTTATAAGCATTCCTACTGAGCAAAAGACTGGAATTCGCTTCCTTGGGAGATTTACAAGTGAGCCAAACATCCATCCTGCCTCCCAGGAAGGCTCATTTATAGTACCAGATGGGGGATCATTGGGTCGAAGCCGCCCAAGGCGTAGGACTAACAACTTTCATAACTCACTTTTCGGGATGATGTCAGCTGCACACACAGGGGAGAAGGATTTGTTCTTCTCTGTCAGGAAAACCACCGAAACCACTCCTAAGGGTACTGGGAATAATCCTAACCCTGCTAGAGTGACAATTAGTTGCCCTGAAAAGGGTGAAGTTTCAGGGAAACTTGTATTACTTCCTGACAGTTTTGAGGAGTTACTGGAGGTTGGTGCCAAGAAATTTGGTCTCACTCCGGCTAAAGTTCTGTGCAAAGATGGAGGAGCTGAAATCGACGATATAGAGGTGATTAGAGACGGTGATCATCTTATTTTTGTCAGTGGCCAAGATGAATCAGAACCTCAAGAATGTAATGGAGAAGATACTCCAACTAAAAAAGATGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008324 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010035 GO:0010053 GO:0010054 GO:0010107 GO:0010119 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016049 GO:0021700 GO:0022622 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0034220 GO:0040007 GO:0042221 GO:0042391 GO:0042802 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071804 GO:0071805 GO:0071944 GO:0080147 GO:0090333 GO:0090558 GO:0090627 GO:0098655 GO:0098660 GO:0098662 GO:0099094 GO:0099402 GO:1901700 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

749

Amino Acids

83.23

Weight (kDa)

5.57

Isoelectric Point (pI)

37.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
cNMP_binding	PF00027	67 - 152	1.2e-15	Cyclic nucleotide-binding domain
Ank_2	PF12796	198 - 289	3.2e-18	Ankyrin repeats (3 copies)
Ank_4	PF13637	198 - 247	1.9e-09	Ankyrin repeats (many copies)
Ank_5	PF13857	213 - 264	1.5e-08	Ankyrin repeats (many copies)
Ank	PF00023	226 - 257	2.4e-07	Ankyrin repeat
Ank_4	PF13637	293 - 344	1.6e-06	Ankyrin repeats (many copies)
Ank_2	PF12796	297 - 375	9e-12	Ankyrin repeats (3 copies)
Ank_5	PF13857	311 - 364	6.3e-08	Ankyrin repeats (many copies)
Ank	PF00023	323 - 354	4.5e-08	Ankyrin repeat
KHA	PF11834	480 - 543	4e-24	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006469)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26650 AT2G26650
fragaria_vesca	FvH4_1g03960 FvH4_1g03960 FvH4_1g03960 FvH4_1g03960 FvH4_1g03960 FvH4_3g32370
malus_domestica	MD02G1037900.v1.1 MD15G1178200.v1.1
prunus_persica	Prupe.7G237400_v2.0.a1 Prupe.7G237400_v2.0.a1 Prupe.7G237400_v2.0.a1
pyrus_communis	pycom15g15990
rosa_chinensis	RchiOBHm_Chr2g0089421
rosa_laevigata	RLG00000016026
rosa_multiflora	Rmu_sc0000197.1_g000013
rosa_roxburghii	Rroxscaffold_2G00151950
rosa_rugosa	Rorug02G0000500 Rorug02G0000600 Rorug02G0000700
rosa_samantha	Rh2AG045800 Rh2BG044300 Rh2CG046200 Rh2DG045700
rosa_wichuraiana	Rw2G003680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1709
AatII	GACGTC	1 cut(s)	1058
AccB1I	GGYRCC	1 cut(s)	2072
AccBSI	CCGCTC	1 cut(s)	636
AccI	GTMKAC	1 cut(s)	1386
AccII	CGCG	2 cut(s)	214, 222
AciI	CCGC	4 cut(s)	220, 634, 821, 1837
AclWI	GGATC	8 cut(s)	549, 1058, 1071, 1131, 1196, 1209, 1365, 1828
AcoI	YGGCCR	1 cut(s)	2182
AcsI	RAATTY	5 cut(s)	473, 977, 1568, 1737, 2081
AcuI	CTGAAG	2 cut(s)	282, 587
AcyI	GRCGYC	2 cut(s)	93, 1055
AfaI	GTAC	7 cut(s)	683, 746, 834, 903, 1332, 1809, 1960
AfiI	CCNNNNNNNGG	3 cut(s)	1817, 2008, 2009
AgsI	TTSAA	7 cut(s)	75, 400, 702, 1048, 1405, 1559, 1676
AhlI	ACTAGT	1 cut(s)	458
AjnI	CCWGG	2 cut(s)	236, 1788
AjuI	GAANNNNNNNTTGG	4 cut(s)	513, 545, 843, 875
Alw21I	GWGCWC	2 cut(s)	114, 1300
Alw26I	GTCTC	9 cut(s)	599, 730, 1046, 1085, 1183, 1308, 1682, 2093, 2148
AlwI	GGATC	8 cut(s)	549, 1058, 1071, 1131, 1196, 1209, 1365, 1828
Ama87I	CYCGRG	1 cut(s)	1037
AoxI	GGCC	2 cut(s)	914, 2182
ApeKI	GCWGC	6 cut(s)	209, 494, 818, 1148, 1247, 1892
ApoI	RAATTY	5 cut(s)	473, 977, 1568, 1737, 2081
Asp700I	GAANNNNTTC	2 cut(s)	261, 1476
AspLEI	GCGC	1 cut(s)	214
AspS9I	GGNCC	1 cut(s)	936
AsuHPI	GGTGA	7 cut(s)	875, 890, 902, 1502, 2024, 2158, 2171
AsuNHI	GCTAGC	1 cut(s)	1105
AvaI	CYCGRG	1 cut(s)	1037
AvaII	GGWCC	1 cut(s)	936
AxyI	CCTNAGG	1 cut(s)	1953
BalI	TGGCCA	1 cut(s)	2184
BamHI	GGATCC	2 cut(s)	1063, 1201
BanI	GGYRCC	1 cut(s)	2072
BauI	CACGAG	1 cut(s)	1110
BbsI	GAAGAC	3 cut(s)	264, 768, 1554
Bbv12I	GWGCWC	2 cut(s)	114, 1300
BbvI	GCAGC	6 cut(s)	221, 481, 805, 1160, 1234, 1879
BccI	CCATC	5 cut(s)	638, 1004, 1785, 1808, 2111
BceAI	ACGGC	1 cut(s)	1539
BciT130I	CCWGG	2 cut(s)	238, 1790
BciVI	GTATCC	1 cut(s)	475
BclI	TGATCA	1 cut(s)	2161
BcoDI	GTCTC	9 cut(s)	599, 730, 1046, 1085, 1183, 1308, 1682, 2093, 2148
BcuI	ACTAGT	1 cut(s)	458
BfaI	CTAG	6 cut(s)	32, 459, 1106, 1506, 1634, 1983
BfmI	CTRYAG	2 cut(s)	536, 789
BfuI	GTATCC	1 cut(s)	475
BglII	AGATCT	1 cut(s)	38
BisI	GCNGC	7 cut(s)	210, 495, 819, 1149, 1248, 1837, 1893
BlsI	GCNGC	7 cut(s)	211, 496, 820, 1150, 1249, 1838, 1894
BmcAI	AGTACT	1 cut(s)	746
Bme1390I	CCNGG	2 cut(s)	238, 1790
Bme18I	GGWCC	1 cut(s)	936
BmeT110I	CYCGRG	1 cut(s)	1037
BmgT120I	GGNCC	1 cut(s)	936
BmiI	GGNNCC	5 cut(s)	518, 1065, 1203, 1519, 2074
BmrFI	CCNGG	2 cut(s)	238, 1790
BmrI	ACTGGG	2 cut(s)	222, 1971
BmsI	GCATC	5 cut(s)	179, 358, 571, 1186, 1259
BmtI	GCTAGC	1 cut(s)	1109
BmuI	ACTGGG	2 cut(s)	222, 1971
BpiI	GAAGAC	3 cut(s)	264, 768, 1554
BpmI	CTGGAG	3 cut(s)	484, 578, 2084
BpuEI	CTTGAG	3 cut(s)	622, 821, 2187
BsaAI	YACGTR	1 cut(s)	292
BsaBI	GATNNNNATC	2 cut(s)	348, 412
BsaHI	GRCGYC	2 cut(s)	93, 1055
BsaI	GGTCTC	3 cut(s)	730, 1682, 2093
BsaJI	CCNNGG	9 cut(s)	403, 451, 1059, 1255, 1521, 1612, 1747, 1788, 1841
BsaXI	ACNNNNNCTCC	2 cut(s)	1479, 1509
Bsc4I	CCNNNNNNNGG	3 cut(s)	1817, 2008, 2009
Bse1I	ACTGG	7 cut(s)	228, 501, 817, 880, 1738, 1966, 2067
Bse21I	CCTNAGG	1 cut(s)	1953
Bse3DI	GCAATG	3 cut(s)	1158, 1434, 1519
Bse8I	GATNNNNATC	2 cut(s)	348, 412
BseBI	CCWGG	2 cut(s)	238, 1790
BseDI	CCNNGG	9 cut(s)	403, 451, 1059, 1255, 1521, 1612, 1747, 1788, 1841
BseGI	GGATG	8 cut(s)	194, 349, 630, 1250, 1420, 1773, 1777, 1887
BseJI	GATNNNNATC	2 cut(s)	348, 412
BseLI	CCNNNNNNNGG	3 cut(s)	1817, 2008, 2009
BseMI	GCAATG	3 cut(s)	1158, 1434, 1519
BseMII	CTCAG	5 cut(s)	741, 1086, 1147, 1434, 1713
BseNI	ACTGG	7 cut(s)	228, 501, 817, 880, 1738, 1966, 2067
BseRI	GAGGAG	4 cut(s)	1025, 1173, 2069, 2136
BseXI	GCAGC	6 cut(s)	221, 481, 805, 1160, 1234, 1879
BseYI	CCCAGC	1 cut(s)	1409
BsgI	GTGCAG	1 cut(s)	1878
Bsh1236I	CGCG	2 cut(s)	214, 222
BshFI	GGCC	2 cut(s)	916, 2184
BshNI	GGYRCC	1 cut(s)	2072
BsiHKAI	GWGCWC	2 cut(s)	114, 1300
BsiHKCI	CYCGRG	1 cut(s)	1037
BsiSI	CCGG	3 cut(s)	84, 961, 2096
BslFI	GGGAC	2 cut(s)	469, 603
BslI	CCNNNNNNNGG	3 cut(s)	1817, 2008, 2009
BsmAI	GTCTC	9 cut(s)	599, 730, 1046, 1085, 1183, 1308, 1682, 2093, 2148
BsmBI	CGTCTC	3 cut(s)	1046, 1183, 2148
BsmFI	GGGAC	2 cut(s)	469, 603
BsmI	GAATGC	2 cut(s)	1411, 1713
BsnI	GGCC	2 cut(s)	916, 2184
Bso31I	GGTCTC	3 cut(s)	730, 1682, 2093
BsoBI	CYCGRG	1 cut(s)	1037
Bsp1286I	GDGCHC	2 cut(s)	114, 1300
Bsp19I	CCATGG	2 cut(s)	451, 1612
BspACI	CCGC	4 cut(s)	220, 634, 821, 1837
BspANI	GGCC	2 cut(s)	916, 2184
BspCNI	CTCAG	5 cut(s)	740, 1087, 1146, 1435, 1714
BspFNI	CGCG	2 cut(s)	214, 222
BspHI	TCATGA	1 cut(s)	1360
BspLI	GGNNCC	5 cut(s)	518, 1065, 1203, 1519, 2074
BspMAI	CTGCAG	1 cut(s)	540
BspOI	GCTAGC	1 cut(s)	1109
BspPI	GGATC	8 cut(s)	549, 1058, 1071, 1131, 1196, 1209, 1365, 1828
BspT107I	GGYRCC	1 cut(s)	2072
BspTNI	GGTCTC	3 cut(s)	730, 1682, 2093
BsrBI	CCGCTC	1 cut(s)	636
BsrDI	GCAATG	3 cut(s)	1158, 1434, 1519
BsrI	ACTGG	7 cut(s)	228, 501, 817, 880, 1738, 1966, 2067
BssECI	CCNNGG	9 cut(s)	403, 451, 1059, 1255, 1521, 1612, 1747, 1788, 1841
BssNI	GRCGYC	2 cut(s)	93, 1055
BssSI	CACGAG	1 cut(s)	1110
BssT1I	CCWWGG	7 cut(s)	403, 451, 1059, 1255, 1612, 1747, 1841
Bst2BI	CACGAG	1 cut(s)	1110
Bst2UI	CCWGG	2 cut(s)	238, 1790
Bst4CI	ACNGT	7 cut(s)	331, 931, 1271, 1310, 1338, 2048, 2159
Bst6I	CTCTTC	2 cut(s)	96, 1650
BstACI	GRCGYC	2 cut(s)	93, 1055
BstBAI	YACGTR	1 cut(s)	292
BstC8I	GCNNGC	5 cut(s)	536, 552, 996, 1107, 1432
BstDEI	CTNAG	7 cut(s)	97, 727, 1095, 1133, 1443, 1722, 1953
BstDSI	CCRYGG	3 cut(s)	451, 1521, 1612
BstF5I	GGATG	8 cut(s)	194, 349, 630, 1250, 1420, 1773, 1777, 1887
BstFNI	CGCG	2 cut(s)	214, 222
BstHHI	GCGC	1 cut(s)	214
BstMAI	GTCTC	9 cut(s)	599, 730, 1046, 1085, 1183, 1308, 1682, 2093, 2148
BstMWI	GCNNNNNNNGC	2 cut(s)	961, 1436
BstNI	CCWGG	2 cut(s)	238, 1790
BstNSI	RCATGY	3 cut(s)	81, 394, 1105
BstSCI	CCNGG	2 cut(s)	236, 1788
BstSFI	CTRYAG	2 cut(s)	536, 789
BstUI	CGCG	2 cut(s)	214, 222
BstV1I	GCAGC	6 cut(s)	221, 481, 805, 1160, 1234, 1879
BstV2I	GAAGAC	3 cut(s)	264, 768, 1554
BstX2I	RGATCY	4 cut(s)	38, 1063, 1123, 1201
BstYI	RGATCY	4 cut(s)	38, 1063, 1123, 1201
Bsu36I	CCTNAGG	1 cut(s)	1953
BsuI	GTATCC	1 cut(s)	475
BsuRI	GGCC	2 cut(s)	916, 2184
BtgI	CCRYGG	3 cut(s)	451, 1521, 1612
BtsCI	GGATG	8 cut(s)	194, 349, 630, 1250, 1420, 1773, 1777, 1887
BtsI	GCAGTG	1 cut(s)	1545
BtsIMutI	CAGTG	3 cut(s)	1334, 1545, 2185
Cac8I	GCNNGC	5 cut(s)	536, 552, 996, 1107, 1432
CciI	TCATGA	1 cut(s)	1360
CfoI	GCGC	1 cut(s)	214
Cfr13I	GGNCC	1 cut(s)	936
CseI	GACGC	1 cut(s)	82
Csp6I	GTAC	7 cut(s)	682, 745, 833, 902, 1331, 1808, 1959
CviAII	CATG	9 cut(s)	78, 391, 452, 1102, 1361, 1484, 1532, 1613, 1652
CviQI	GTAC	7 cut(s)	682, 745, 833, 902, 1331, 1808, 1959
DdeI	CTNAG	7 cut(s)	97, 727, 1095, 1133, 1443, 1722, 1953
EaeI	YGGCCR	1 cut(s)	2182
Eam1104I	CTCTTC	2 cut(s)	96, 1650
EarI	CTCTTC	2 cut(s)	96, 1650
Eco130I	CCWWGG	7 cut(s)	403, 451, 1059, 1255, 1612, 1747, 1841
Eco31I	GGTCTC	3 cut(s)	730, 1682, 2093
Eco32I	GATATC	1 cut(s)	1594
Eco47I	GGWCC	1 cut(s)	936
Eco57I	CTGAAG	2 cut(s)	282, 587
Eco81I	CCTNAGG	1 cut(s)	1953
Eco88I	CYCGRG	1 cut(s)	1037
EcoRI	GAATTC	1 cut(s)	1737
EcoRII	CCWGG	2 cut(s)	236, 1788
EcoRV	GATATC	1 cut(s)	1594
EcoT14I	CCWWGG	7 cut(s)	403, 451, 1059, 1255, 1612, 1747, 1841
ErhI	CCWWGG	7 cut(s)	403, 451, 1059, 1255, 1612, 1747, 1841
Esp3I	CGTCTC	3 cut(s)	1046, 1183, 2148
FaeI	CATG	9 cut(s)	81, 394, 455, 1105, 1364, 1487, 1535, 1616, 1655
FalI	AAGNNNNNCTT	6 cut(s)	249, 281, 594, 626, 668, 700
FaqI	GGGAC	2 cut(s)	469, 603
FatI	CATG	9 cut(s)	77, 390, 451, 1101, 1360, 1483, 1531, 1612, 1651
FbaI	TGATCA	1 cut(s)	2161
FblI	GTMKAC	1 cut(s)	1386
Fnu4HI	GCNGC	7 cut(s)	210, 495, 819, 1149, 1248, 1837, 1893
FokI	GGATG	8 cut(s)	201, 336, 617, 1237, 1427, 1760, 1764, 1894
Fsp4HI	GCNGC	7 cut(s)	210, 495, 819, 1149, 1248, 1837, 1893
FspBI	CTAG	6 cut(s)	32, 459, 1106, 1506, 1634, 1983
GlaI	GCGC	1 cut(s)	213
GluI	GCNGC	7 cut(s)	210, 495, 819, 1149, 1248, 1837, 1893
GsaI	CCCAGC	1 cut(s)	1413
GsuI	CTGGAG	3 cut(s)	484, 578, 2084
HaeIII	GGCC	2 cut(s)	916, 2184
HapII	CCGG	3 cut(s)	84, 961, 2096
HgaI	GACGC	1 cut(s)	82
HhaI	GCGC	1 cut(s)	214
Hin1I	GRCGYC	2 cut(s)	93, 1055
Hin1II	CATG	9 cut(s)	81, 394, 455, 1105, 1364, 1487, 1535, 1616, 1655
Hin6I	GCGC	1 cut(s)	212
HinP1I	GCGC	1 cut(s)	212
HindIII	AAGCTT	1 cut(s)	172
HinfI	GANTC	8 cut(s)	283, 591, 607, 614, 949, 1211, 1316, 2192
HpaII	CCGG	3 cut(s)	84, 961, 2096
HphI	GGTGA	7 cut(s)	875, 890, 902, 1502, 2024, 2158, 2171
Hpy166II	GTNNAC	2 cut(s)	682, 1387
Hpy188I	TCNGA	8 cut(s)	24, 567, 730, 1206, 1216, 1321, 1546, 2197
Hpy8I	GTNNAC	2 cut(s)	682, 1387
Hpy99I	CGWCG	1 cut(s)	2138
HpyAV	CCTTC	5 cut(s)	29, 212, 1348, 1787, 1903
HpyCH4III	ACNGT	7 cut(s)	331, 931, 1271, 1310, 1338, 2048, 2159
HpyCH4IV	ACGT	2 cut(s)	291, 1055
HpyF10VI	GCNNNNNNNGC	2 cut(s)	961, 1436
HpyF3I	CTNAG	7 cut(s)	97, 727, 1095, 1133, 1443, 1722, 1953
HpySE526I	ACGT	2 cut(s)	291, 1055
Hsp92I	GRCGYC	2 cut(s)	93, 1055
Hsp92II	CATG	9 cut(s)	81, 394, 455, 1105, 1364, 1487, 1535, 1616, 1655
HspAI	GCGC	1 cut(s)	212
Ksp22I	TGATCA	1 cut(s)	2161
LmnI	GCTCC	4 cut(s)	86, 845, 1295, 2123
Lsp1109I	GCAGC	6 cut(s)	221, 481, 805, 1160, 1234, 1879
LweI	GCATC	5 cut(s)	179, 358, 571, 1186, 1259
MaeI	CTAG	6 cut(s)	32, 459, 1106, 1506, 1634, 1983
MaeII	ACGT	2 cut(s)	291, 1055
MaeIII	GTNAC	7 cut(s)	215, 230, 292, 808, 1495, 1987, 2058
MbiI	CCGCTC	1 cut(s)	636
MflI	RGATCY	4 cut(s)	38, 1063, 1123, 1201
MhlI	GDGCHC	2 cut(s)	114, 1300
MlsI	TGGCCA	1 cut(s)	2184
MluNI	TGGCCA	1 cut(s)	2184
MlyI	GAGTC	4 cut(s)	585, 601, 958, 1310
MmeI	TCCRAC	4 cut(s)	985, 1068, 1239, 1437
Mox20I	TGGCCA	1 cut(s)	2184
MroXI	GAANNNNTTC	2 cut(s)	261, 1476
MscI	TGGCCA	1 cut(s)	2184
MseI	TTAA	1 cut(s)	983
MslI	CAYNNNNRTG	1 cut(s)	1488
Msp20I	TGGCCA	1 cut(s)	2184
MspA1I	CMGCKG	1 cut(s)	1892
MspI	CCGG	3 cut(s)	84, 961, 2096
MspR9I	CCNGG	2 cut(s)	238, 1790
Mva1269I	GAATGC	2 cut(s)	1411, 1713
MvaI	CCWGG	2 cut(s)	238, 1790
MvnI	CGCG	2 cut(s)	214, 222
MwoI	GCNNNNNNNGC	2 cut(s)	961, 1436
NcoI	CCATGG	2 cut(s)	451, 1612
NheI	GCTAGC	1 cut(s)	1105
NlaIII	CATG	9 cut(s)	81, 394, 455, 1105, 1364, 1487, 1535, 1616, 1655
NlaIV	GGNNCC	5 cut(s)	518, 1065, 1203, 1519, 2074
NmeAIII	GCCGAG	1 cut(s)	766
NmuCI	GTSAC	3 cut(s)	292, 1495, 1987
NspI	RCATGY	3 cut(s)	81, 394, 1105
PaeR7I	CTCGAG	1 cut(s)	1037
PagI	TCATGA	1 cut(s)	1360
PcsI	WCGNNNNNNNCGW	1 cut(s)	1542
PctI	GAATGC	2 cut(s)	1411, 1713
PdmI	GAANNNNTTC	2 cut(s)	261, 1476
PfeI	GAWTC	4 cut(s)	283, 614, 1211, 2192
PkrI	GCNGC	7 cut(s)	211, 496, 820, 1150, 1249, 1838, 1894
PleI	GAGTC	4 cut(s)	585, 601, 957, 1310
PpsI	GAGTC	4 cut(s)	585, 601, 957, 1310
Ppu21I	YACGTR	1 cut(s)	292
PsiI	TTATAA	1 cut(s)	1709
Psp6I	CCWGG	2 cut(s)	236, 1788
PspFI	CCCAGC	1 cut(s)	1409
PspGI	CCWGG	2 cut(s)	236, 1788
PspN4I	GGNNCC	5 cut(s)	518, 1065, 1203, 1519, 2074
PspPI	GGNCC	1 cut(s)	936
PspXI	VCTCGAGB	1 cut(s)	1037
PsrI	GAACNNNNNNTAC	2 cut(s)	133, 165
PstI	CTGCAG	1 cut(s)	540
PsuI	RGATCY	4 cut(s)	38, 1063, 1123, 1201
PvuII	CAGCTG	1 cut(s)	1892
RsaI	GTAC	7 cut(s)	683, 746, 834, 903, 1332, 1809, 1960
RsaNI	GTAC	7 cut(s)	682, 745, 833, 902, 1331, 1808, 1959
RseI	CAYNNNNRTG	1 cut(s)	1488
SaqAI	TTAA	1 cut(s)	983
SatI	GCNGC	7 cut(s)	210, 495, 819, 1149, 1248, 1837, 1893
Sau96I	GGNCC	1 cut(s)	936
SbfI	CCTGCAGG	1 cut(s)	540
ScaI	AGTACT	1 cut(s)	746
SchI	GAGTC	4 cut(s)	585, 601, 958, 1310
ScrFI	CCNGG	2 cut(s)	238, 1790
SdaI	CCTGCAGG	1 cut(s)	540
SduI	GDGCHC	2 cut(s)	114, 1300
SfaNI	GCATC	5 cut(s)	179, 358, 571, 1186, 1259
SfcI	CTRYAG	2 cut(s)	536, 789
Sfr274I	CTCGAG	1 cut(s)	1037
SinI	GGWCC	1 cut(s)	936
SlaI	CTCGAG	1 cut(s)	1037
SmiMI	CAYNNNNRTG	1 cut(s)	1488
SmlI	CTYRAG	4 cut(s)	637, 836, 1037, 2202
SmoI	CTYRAG	4 cut(s)	637, 836, 1037, 2202
SpeI	ACTAGT	1 cut(s)	458
Sse8387I	CCTGCAGG	1 cut(s)	540
SsiI	CCGC	4 cut(s)	220, 634, 821, 1837
SspI	AATATT	1 cut(s)	771
SspMI	CTAG	6 cut(s)	32, 459, 1106, 1506, 1634, 1983
StyD4I	CCNGG	2 cut(s)	236, 1788
StyI	CCWWGG	7 cut(s)	403, 451, 1059, 1255, 1612, 1747, 1841
TaaI	ACNGT	7 cut(s)	331, 931, 1271, 1310, 1338, 2048, 2159
TaiI	ACGT	2 cut(s)	294, 1058
TaqI	TCGA	9 cut(s)	286, 463, 672, 1038, 1480, 1596, 1679, 1831, 2133
TatI	WGTACW	2 cut(s)	681, 744
TauI	GCSGC	1 cut(s)	1839
TfiI	GAWTC	4 cut(s)	283, 614, 1211, 2192
Tru1I	TTAA	1 cut(s)	983
Tru9I	TTAA	1 cut(s)	983
TscAI	CASTG	3 cut(s)	1341, 1545, 2185
TseFI	GTSAC	3 cut(s)	292, 1495, 1987
TseI	GCWGC	6 cut(s)	209, 494, 818, 1148, 1247, 1892
Tsp45I	GTSAC	3 cut(s)	292, 1495, 1987
TspDTI	ATGAA	9 cut(s)	257, 749, 795, 1040, 1224, 1377, 1668, 1853, 2205
TspRI	CASTG	3 cut(s)	1341, 1545, 2185
VpaK11BI	GGWCC	1 cut(s)	936
XapI	RAATTY	5 cut(s)	473, 977, 1568, 1737, 2081
XceI	RCATGY	3 cut(s)	81, 394, 1105
XhoI	CTCGAG	1 cut(s)	1037
XmiI	GTMKAC	1 cut(s)	1386
XmnI	GAANNNNTTC	2 cut(s)	261, 1476
XspI	CTAG	6 cut(s)	32, 459, 1106, 1506, 1634, 1983
ZraI	GACGTC	1 cut(s)	1056
ZrmI	AGTACT	1 cut(s)	746

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.