Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0006469

Physical Location & Seq

Genomic Coordinates Forward (+)

3650032 .. 3656717

Gene Structure 6686 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ46472

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2676 bp

ATGGCGTTTACTGGTGTTTCAATGTGCGGCCAAGAGCAGCTAGACTTGTCCAGAGACGGCAGCCACTACAGTCTCTCCACCGGAATACTGCCGTCGCTCGGCGCCAGGTCTAGTAATCGGAGAGTCAAGCTCAGCCGGTTTATTGTTTCGCCTTACGACCGGCGATACAGAATTTGGGAGACATTTCTTGTTGTTCTGGTCATCTACACTGCTTGGGTCTCCCCCTTCGAATTTGGCTTTCTTAACAAACCACAGGGACCACTTTCCATTATTGACAATATTGTCAATGGCTTCTTTGCTATAGATATTGTTCTTACATTCTTTGTGGCATACCTGGATAAGGCTACATATCTACTTGTTGACGATCAAAAGAAGATTGCTTGGAAGTACGCAAGTACGTGGTTGATCTTTGATGTCATATCCACAATTCCTACTGAACTTGCTAGGAAGATTTCCCCTAAACCTTTCCGGTCATATGGCTTTTTCAACATGCTCCGTCTTTGGCGTCTTAGAAGAGTTAGTGCTCTATTTTCCAGATTGGAGAAAGATAGGAACTATAATTACTTTTGGGTTCGTTGTGCAAAACTTATTTGTGTCACCCTGTTTGCAGTTCACTGTGCTGGATGCTTCTATTACCTTCTTGCAGCCCGTTACCGTGACCCCAAGAACACATGGATTGGAAAAACAATGGAAGACTTTCTTCATCAAAGTCTGTGGATTCGATATGTGACTTCAGTGTATTGGTCTATCACTACACTCACAACCGTTGGCTACGGAGATTTGCATCCTGTCAATACAAGGGAGATGATCTTTGACATATTCTACATGCTTTTCAACCTTGGCTTGACATCATACTTGATTGGAAATATGACCAACTTGGTTGTCCATGGGACCAGTCGAACACGAAAATTTAGGGATACCATACAAGCTGCCTCCAGTTTCGCACAGAGGAACCAACTGCCTGTTCGCCTGCAGGATCAGATGCTTGCCCACTTGTGTCTGAAGTTCAGGACAGATGCAGAGGGACTCCAGCAACAAGAGACTCTTGATTCACTTCCGAAAGCGATACGCTCAAGTATTTCACATTATCTGTTTTACTCTCTTGTCGATAAGGTGTACTTGTTTCATGGGGTTTCAAATGACTTGCTTTTTCAGTTGGTCTCAGAGATGAAAGCCGAGTACTTTCCTCCCAAAGAAGATGTAATATTGCAGAATGAAGCACCAACAGATTTCTACATACTTGTCACTGGTGCTGCGGATTTAGTGGTTCTCAAGAATGGAGTTGAACAGGTCATTGGTGAGGCAAAAACTGGTGATCTCATTGGTGAAATTGGGGTACTCTGTTATAGGCCACAGCTTTTTACCGTTAGGACCAAAAGATTGAGTCAGCTACTCCGGCTGAACCGAACTACATTCTTAAGCATAGTTCAAGCTAATGTTGGGGATGGAACTATAATAATGAATAACCTCCTCCAGCATTTGAAAGAGGTCAAGGATCCATATATGGAGGGAGTTTTGCTGGAGACTGAGAACATGCTAGCACGTGGTAGACTGGATCTACCTCTCAGTCTATGCTTTGCAGCAATTAGAGGAGATGACTTGCTGTTGCATCAGTTGTTGAGACGGGGTTTGGATCCGAATGAATCAGACAATAATGGCAGAACAGCTCTGCATATAGCTGCATCCCAAGGTAGTGAGAACTGTGTGCTTCTACTATTGGACTATGGAGCACTTCCTAACAGTAGAGACTCGGAGGGAAGTGTACCACTTTGGGAGGCAATACAGAAGGATCATGAATCGATTGCCAAACTGCTGATGGACAACGGGGCAACTTTGACTGTTGGGGATGTTGGTCACTTTGCTTGCACTGCTGCTGAGCAAAACAGGTTGGACTTGCTCAATGAAATAGTTCAACATGGAGGAGATGTCACAGCTCCTAGAAGCAATGGAACCACAGCTCTCCATGTCGCAGTGTCCGAAGACAACATTGAAATTGTGAAATTCCTTTTGGACAAAGGGGCAGATATTGACAAACCAGACCTCCATGGCTGGACCGCGAGAGCTCTAGCCGACCAACAAGGACATGAAGAGATAAAGAACCTTTTTCAATCTGGAAAAGAGCCCAAAGCTCAATCTGTTATCACCATTCCTACTGTGCAAAAGCCTGGAATTCGCTTCCTAGGGAGATTTACAAGCGAGCCAAACATCCATCCTCCTTCCCAGGAAGGCTCATTTCTAGTAGCAGATGGGGGATCATGGGGTCGAAGCCGCCCAAGGCGTAGGACTAACAACTTCCACAACTCACTTTTTGGGATGATGTCAGCTGCACACACAGGGGAGAAGGATTTGTTCTTCTCCTCAATTAGGGAGACCGTCGAAACCACTCCTGAGGGTATTGGGAGTAATCCTAACCCTGCTAGAGTGACAATTAGTTGCCCTGAAAATAGTGAAGTTTCAGGGAAACTTGTATTACTTCCTGCCAGTTTTGAGGAGTTACTCGAGGTTGGTGCTAAGAAATTCGGTTTCACTCCGGCTAAAGTTCTGAGCAAAGATGGAGGAGCTGAAATTGACGATATAGAGGTGATTAGAGACGGTGATCATCTTATTTTTGTCAGTGGCCAAGATGAACCTCAAGAATGTACTAGCGAAGATACTCCGACTAAAAAAGATGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008324 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010035 GO:0010053 GO:0010054 GO:0010107 GO:0010119 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016049 GO:0021700 GO:0022622 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0034220 GO:0040007 GO:0042221 GO:0042391 GO:0042802 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071804 GO:0071805 GO:0071944 GO:0080147 GO:0090333 GO:0090558 GO:0090627 GO:0098655 GO:0098660 GO:0098662 GO:0099094 GO:0099402 GO:1901700 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

891

Amino Acids

100.02

Weight (kDa)

6.2

Isoelectric Point (pI)

40.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	56 - 301	3.1e-44	Ion transport protein
Ion_trans_2	PF07885	204 - 294	3.7e-13	Ion channel
cNMP_binding	PF00027	394 - 479	2.6e-14	Cyclic nucleotide-binding domain
Ank_2	PF12796	524 - 614	1.2e-16	Ankyrin repeats (3 copies)
Ank_4	PF13637	524 - 573	7.3e-09	Ankyrin repeats (many copies)
Ank_5	PF13857	539 - 590	4e-08	Ankyrin repeats (many copies)
Ank	PF00023	552 - 583	4.1e-07	Ankyrin repeat
Ank_2	PF12796	623 - 710	5.9e-12	Ankyrin repeats (3 copies)
Ank_4	PF13637	623 - 670	1.2e-07	Ankyrin repeats (many copies)
Ank_5	PF13857	637 - 690	9.6e-08	Ankyrin repeats (many copies)
Ank	PF00023	649 - 680	3.8e-08	Ankyrin repeat
KHA	PF11834	807 - 870	6.6e-24	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006469)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26650 AT2G26650
fragaria_vesca	FvH4_1g03960 FvH4_1g03960 FvH4_1g03960 FvH4_1g03960 FvH4_1g03960 FvH4_3g32370
malus_domestica	MD02G1037900.v1.1 MD15G1178200.v1.1
prunus_persica	Prupe.7G237400_v2.0.a1 Prupe.7G237400_v2.0.a1 Prupe.7G237400_v2.0.a1
pyrus_communis	pycom15g15990
rosa_chinensis	RchiOBHm_Chr2g0089421
rosa_laevigata	RLG00000016026
rosa_multiflora	Rmu_sc0000197.1_g000013
rosa_roxburghii	Rroxscaffold_2G00151950
rosa_rugosa	Rorug02G0000500 Rorug02G0000600 Rorug02G0000700
rosa_samantha	Rh2AG045800 Rh2BG044300 Rh2CG046200 Rh2DG045700
rosa_wichuraiana	Rw2G003680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	281
AccB1I	GGYRCC	1 cut(s)	101
AccI	GTMKAC	1 cut(s)	1549
AccII	CGCG	1 cut(s)	2057
AciI	CCGC	4 cut(s)	27, 1256, 2055, 2269
AclWI	GGATC	8 cut(s)	984, 1490, 1503, 1563, 1628, 1641, 1797, 2260
AcoI	YGGCCR	2 cut(s)	28, 2617
AcsI	RAATTY	6 cut(s)	171, 230, 908, 2000, 2169, 2516
AcuI	CTGAAG	2 cut(s)	717, 1022
AcvI	CACGTG	1 cut(s)	1544
AcyI	GRCGYC	2 cut(s)	102, 505
AfaI	GTAC	7 cut(s)	389, 397, 1118, 1181, 1338, 1764, 2641
AfiI	CCNNNNNNNGG	3 cut(s)	98, 340, 2364
AflII	CTTAAG	1 cut(s)	1417
AjnI	CCWGG	4 cut(s)	104, 333, 2164, 2220
AjuI	GAANNNNNNNTTGG	6 cut(s)	948, 980, 1278, 1310, 1991, 2023
Alw21I	GWGCWC	3 cut(s)	526, 1732, 2065
AlwI	GGATC	8 cut(s)	984, 1490, 1503, 1563, 1628, 1641, 1797, 2260
Ama87I	CYCGRG	1 cut(s)	2498
AoxI	GGCC	3 cut(s)	28, 1349, 2617
ApeKI	GCWGC	9 cut(s)	37, 60, 644, 929, 1253, 1580, 1679, 1871, 2324
ApoI	RAATTY	6 cut(s)	171, 230, 908, 2000, 2169, 2516
Asp700I	GAANNNNTTC	2 cut(s)	696, 1908
AspA2I	CCTAGG	1 cut(s)	2179
AspLEI	GCGC	1 cut(s)	104
AspS9I	GGNCC	4 cut(s)	257, 891, 1371, 2052
AsuHPI	GGTGA	7 cut(s)	589, 1310, 1325, 1337, 2134, 2593, 2606
AsuII	TTCGAA	1 cut(s)	228
AsuNHI	GCTAGC	1 cut(s)	1537
AvaI	CYCGRG	1 cut(s)	2498
AvaII	GGWCC	4 cut(s)	257, 891, 1371, 2052
AvrII	CCTAGG	1 cut(s)	2179
AxyI	CCTNAGG	1 cut(s)	2388
BalI	TGGCCA	1 cut(s)	2619
BamHI	GGATCC	2 cut(s)	1495, 1633
BanI	GGYRCC	1 cut(s)	101
BanII	GRGCYC	2 cut(s)	2065, 2124
BbrPI	CACGTG	1 cut(s)	1544
BbsI	GAAGAC	2 cut(s)	699, 1986
Bbv12I	GWGCWC	3 cut(s)	526, 1732, 2065
BbvI	GCAGC	9 cut(s)	49, 72, 656, 916, 1240, 1592, 1666, 1858, 2311
BccI	CCATC	5 cut(s)	1439, 1810, 2217, 2240, 2546
BceAI	ACGGC	2 cut(s)	73, 76
BcgI	CGANNNNNNTGC	2 cut(s)	2489, 2523
BciT130I	CCWGG	4 cut(s)	106, 335, 2166, 2222
BciVI	GTATCC	1 cut(s)	910
BclI	TGATCA	1 cut(s)	2596
BfmI	CTRYAG	3 cut(s)	67, 300, 971
BfoI	RGCGCY	1 cut(s)	105
BfrI	CTTAAG	1 cut(s)	1417
BfuI	GTATCC	1 cut(s)	910
BlnI	CCTAGG	1 cut(s)	2179
BlpI	GCTNAGC	2 cut(s)	131, 1875
BmcAI	AGTACT	1 cut(s)	1181
Bme1390I	CCNGG	4 cut(s)	106, 335, 2166, 2222
Bme18I	GGWCC	4 cut(s)	257, 891, 1371, 2052
BmeT110I	CYCGRG	1 cut(s)	2498
BmgT120I	GGNCC	4 cut(s)	257, 891, 1371, 2052
BmiI	GGNNCC	7 cut(s)	103, 258, 892, 953, 1497, 1635, 1951
BmrFI	CCNGG	4 cut(s)	106, 335, 2166, 2222
BmsI	GCATC	6 cut(s)	614, 793, 972, 1006, 1618, 1691
BmtI	GCTAGC	1 cut(s)	1541
BpiI	GAAGAC	2 cut(s)	699, 1986
BplI	GAGNNNNNCTC	2 cut(s)	114, 146
BpmI	CTGGAG	4 cut(s)	919, 1013, 1457, 1541
Bpu1102I	GCTNAGC	2 cut(s)	131, 1875
Bpu14I	TTCGAA	1 cut(s)	228
BpuEI	CTTGAG	3 cut(s)	1057, 1256, 2616
Bsa29I	ATCGAT	1 cut(s)	1799
BsaAI	YACGTR	2 cut(s)	399, 1544
BsaBI	GATNNNNATC	1 cut(s)	783
BsaHI	GRCGYC	2 cut(s)	102, 505
BsaI	GGTCTC	3 cut(s)	223, 1165, 2363
BsaJI	CCNNGG	7 cut(s)	838, 886, 1687, 2044, 2179, 2220, 2273
BsaWI	WCCGGW	2 cut(s)	80, 468
BsaXI	ACNNNNNCTCC	6 cut(s)	59, 89, 1911, 1941, 2025, 2055
Bsc4I	CCNNNNNNNGG	3 cut(s)	98, 340, 2364
Bse118I	RCCGGY	2 cut(s)	135, 159
Bse1I	ACTGG	7 cut(s)	16, 894, 936, 1252, 1315, 1557, 2481
Bse21I	CCTNAGG	1 cut(s)	2388
Bse3DI	GCAATG	1 cut(s)	1951
Bse8I	GATNNNNATC	1 cut(s)	783
BseBI	CCWGG	4 cut(s)	106, 335, 2166, 2222
BseCI	ATCGAT	1 cut(s)	1799
BseDI	CCNNGG	7 cut(s)	838, 886, 1687, 2044, 2179, 2220, 2273
BseGI	GGATG	8 cut(s)	629, 784, 1450, 1682, 1852, 2205, 2209, 2319
BseJI	GATNNNNATC	1 cut(s)	783
BseLI	CCNNNNNNNGG	3 cut(s)	98, 340, 2364
BseMI	GCAATG	1 cut(s)	1951
BseMII	CTCAG	7 cut(s)	145, 1176, 1518, 1579, 1866, 2379, 2534
BseNI	ACTGG	7 cut(s)	16, 894, 936, 1252, 1315, 1557, 2481
BseRI	GAGGAG	6 cut(s)	1460, 1605, 1935, 2347, 2504, 2571
BseXI	GCAGC	9 cut(s)	49, 72, 656, 916, 1240, 1592, 1666, 1858, 2311
BsgI	GTGCAG	1 cut(s)	2310
Bsh1236I	CGCG	1 cut(s)	2057
Bsh1285I	CGRYCG	1 cut(s)	160
BshFI	GGCC	3 cut(s)	30, 1351, 2619
BshNI	GGYRCC	1 cut(s)	101
BshVI	ATCGAT	1 cut(s)	1799
BsiEI	CGRYCG	1 cut(s)	160
BsiHKAI	GWGCWC	3 cut(s)	526, 1732, 2065
BsiHKCI	CYCGRG	1 cut(s)	2498
BsiSI	CCGG	6 cut(s)	81, 136, 160, 469, 1396, 2531
BslFI	GGGAC	3 cut(s)	270, 904, 1038
BslI	CCNNNNNNNGG	3 cut(s)	98, 340, 2364
BsmBI	CGTCTC	3 cut(s)	48, 1615, 2583
BsmFI	GGGAC	3 cut(s)	270, 904, 1038
BsnI	GGCC	3 cut(s)	30, 1351, 2619
Bso31I	GGTCTC	3 cut(s)	223, 1165, 2363
BsoBI	CYCGRG	1 cut(s)	2498
Bsp119I	TTCGAA	1 cut(s)	228
Bsp1286I	GDGCHC	4 cut(s)	526, 1732, 2065, 2124
Bsp1720I	GCTNAGC	2 cut(s)	131, 1875
Bsp19I	CCATGG	2 cut(s)	886, 2044
BspACI	CCGC	4 cut(s)	27, 1256, 2055, 2269
BspANI	GGCC	3 cut(s)	30, 1351, 2619
BspCNI	CTCAG	7 cut(s)	144, 1175, 1519, 1578, 1867, 2380, 2535
BspDI	ATCGAT	1 cut(s)	1799
BspFNI	CGCG	1 cut(s)	2057
BspHI	TCATGA	1 cut(s)	1792
BspLI	GGNNCC	7 cut(s)	103, 258, 892, 953, 1497, 1635, 1951
BspMAI	CTGCAG	1 cut(s)	975
BspOI	GCTAGC	1 cut(s)	1541
BspPI	GGATC	8 cut(s)	984, 1490, 1503, 1563, 1628, 1641, 1797, 2260
BspT104I	TTCGAA	1 cut(s)	228
BspT107I	GGYRCC	1 cut(s)	101
BspTI	CTTAAG	1 cut(s)	1417
BspTNI	GGTCTC	3 cut(s)	223, 1165, 2363
BsrDI	GCAATG	1 cut(s)	1951
BsrFI	RCCGGY	2 cut(s)	135, 159
BsrI	ACTGG	7 cut(s)	16, 894, 936, 1252, 1315, 1557, 2481
BssAI	RCCGGY	2 cut(s)	135, 159
BssECI	CCNNGG	7 cut(s)	838, 886, 1687, 2044, 2179, 2220, 2273
BssNI	GRCGYC	2 cut(s)	102, 505
BssT1I	CCWWGG	6 cut(s)	838, 886, 1687, 2044, 2179, 2273
Bst2UI	CCWGG	4 cut(s)	106, 335, 2166, 2222
Bst6I	CTCTTC	2 cut(s)	508, 2082
BstACI	GRCGYC	2 cut(s)	102, 505
BstAFI	CTTAAG	1 cut(s)	1417
BstBAI	YACGTR	2 cut(s)	399, 1544
BstBI	TTCGAA	1 cut(s)	228
BstC8I	GCNNGC	5 cut(s)	971, 987, 1539, 1864, 2198
BstDEI	CTNAG	9 cut(s)	131, 509, 1162, 1527, 1565, 1875, 2388, 2511, 2543
BstDSI	CCRYGG	2 cut(s)	886, 2044
BstENI	CCTNNNNNAGG	2 cut(s)	338, 2362
BstF5I	GGATG	8 cut(s)	629, 784, 1450, 1682, 1852, 2205, 2209, 2319
BstFNI	CGCG	1 cut(s)	2057
BstH2I	RGCGCY	1 cut(s)	105
BstHHI	GCGC	1 cut(s)	104
BstMCI	CGRYCG	1 cut(s)	160
BstMWI	GCNNNNNNNGC	2 cut(s)	1396, 1868
BstNI	CCWGG	4 cut(s)	106, 335, 2166, 2222
BstNSI	RCATGY	3 cut(s)	493, 829, 1537
BstSCI	CCNGG	4 cut(s)	104, 333, 2164, 2220
BstSFI	CTRYAG	3 cut(s)	67, 300, 971
BstUI	CGCG	1 cut(s)	2057
BstV1I	GCAGC	9 cut(s)	49, 72, 656, 916, 1240, 1592, 1666, 1858, 2311
BstV2I	GAAGAC	2 cut(s)	699, 1986
BstX2I	RGATCY	3 cut(s)	1495, 1555, 1633
BstYI	RGATCY	3 cut(s)	1495, 1555, 1633
Bsu15I	ATCGAT	1 cut(s)	1799
Bsu36I	CCTNAGG	1 cut(s)	2388
BsuI	GTATCC	1 cut(s)	910
BsuRI	GGCC	3 cut(s)	30, 1351, 2619
BsuTUI	ATCGAT	1 cut(s)	1799
BtgI	CCRYGG	2 cut(s)	886, 2044
BtsCI	GGATG	8 cut(s)	629, 784, 1450, 1682, 1852, 2205, 2209, 2319
BtsI	GCAGTG	3 cut(s)	207, 1866, 1977
BtsIMutI	CAGTG	7 cut(s)	207, 613, 741, 1245, 1866, 1977, 2620
Cac8I	GCNNGC	5 cut(s)	971, 987, 1539, 1864, 2198
CciI	TCATGA	1 cut(s)	1792
CfoI	GCGC	1 cut(s)	104
Cfr10I	RCCGGY	2 cut(s)	135, 159
Cfr13I	GGNCC	4 cut(s)	257, 891, 1371, 2052
ClaI	ATCGAT	1 cut(s)	1799
CseI	GACGC	1 cut(s)	494
Csp6I	GTAC	7 cut(s)	388, 396, 1117, 1180, 1337, 1763, 2640
CspCI	CAANNNNNGTGG	2 cut(s)	695, 730
CviQI	GTAC	7 cut(s)	388, 396, 1117, 1180, 1337, 1763, 2640
DdeI	CTNAG	9 cut(s)	131, 509, 1162, 1527, 1565, 1875, 2388, 2511, 2543
DinI	GGCGCC	1 cut(s)	103
DrdI	GACNNNNNNGTC	1 cut(s)	281
DseDI	GACNNNNNNGTC	1 cut(s)	281
EaeI	YGGCCR	2 cut(s)	28, 2617
Eam1104I	CTCTTC	2 cut(s)	508, 2082
EarI	CTCTTC	2 cut(s)	508, 2082
Ecl136II	GAGCTC	1 cut(s)	2063
Eco130I	CCWWGG	6 cut(s)	838, 886, 1687, 2044, 2179, 2273
Eco24I	GRGCYC	2 cut(s)	2065, 2124
Eco31I	GGTCTC	3 cut(s)	223, 1165, 2363
Eco47I	GGWCC	4 cut(s)	257, 891, 1371, 2052
Eco53kI	GAGCTC	1 cut(s)	2063
Eco57I	CTGAAG	2 cut(s)	717, 1022
Eco72I	CACGTG	1 cut(s)	1544
Eco81I	CCTNAGG	1 cut(s)	2388
Eco88I	CYCGRG	1 cut(s)	2498
EcoICRI	GAGCTC	1 cut(s)	2063
EcoNI	CCTNNNNNAGG	2 cut(s)	338, 2362
EcoRI	GAATTC	1 cut(s)	2169
EcoRII	CCWGG	4 cut(s)	104, 333, 2164, 2220
EcoT14I	CCWWGG	6 cut(s)	838, 886, 1687, 2044, 2179, 2273
EcoT38I	GRGCYC	2 cut(s)	2065, 2124
EgeI	GGCGCC	1 cut(s)	103
EheI	GGCGCC	1 cut(s)	103
ErhI	CCWWGG	6 cut(s)	838, 886, 1687, 2044, 2179, 2273
Esp3I	CGTCTC	3 cut(s)	48, 1615, 2583
FalI	AAGNNNNNCTT	6 cut(s)	684, 716, 1029, 1061, 1103, 1135
FaqI	GGGAC	3 cut(s)	270, 904, 1038
FauNDI	CATATG	1 cut(s)	475
FbaI	TGATCA	1 cut(s)	2596
FblI	GTMKAC	1 cut(s)	1549
FokI	GGATG	8 cut(s)	636, 771, 1457, 1669, 1859, 2192, 2196, 2326
FriOI	GRGCYC	2 cut(s)	2065, 2124
GlaI	GCGC	1 cut(s)	103
GsuI	CTGGAG	4 cut(s)	919, 1013, 1457, 1541
HaeII	RGCGCY	1 cut(s)	105
HaeIII	GGCC	3 cut(s)	30, 1351, 2619
HapII	CCGG	6 cut(s)	81, 136, 160, 469, 1396, 2531
HgaI	GACGC	1 cut(s)	494
HhaI	GCGC	1 cut(s)	104
Hin1I	GRCGYC	2 cut(s)	102, 505
Hin6I	GCGC	1 cut(s)	102
HinP1I	GCGC	1 cut(s)	102
HincII	GTYRAC	1 cut(s)	361
HindII	GTYRAC	1 cut(s)	361
HinfI	GANTC	9 cut(s)	123, 718, 1026, 1042, 1049, 1384, 1643, 1748, 1796
HpaII	CCGG	6 cut(s)	81, 136, 160, 469, 1396, 2531
HphI	GGTGA	7 cut(s)	589, 1310, 1325, 1337, 2134, 2593, 2606
Hpy166II	GTNNAC	6 cut(s)	9, 361, 613, 1117, 1550, 1763
Hpy188III	TCNNGA	9 cut(s)	51, 534, 1009, 1046, 1273, 1793, 2112, 2387, 2633
Hpy8I	GTNNAC	6 cut(s)	9, 361, 613, 1117, 1550, 1763
Hpy99I	CGWCG	2 cut(s)	97, 2378
HpyAV	CCTTC	6 cut(s)	235, 647, 1780, 2219, 2226, 2335
HpyCH4IV	ACGT	2 cut(s)	398, 1543
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1396, 1868
HpyF3I	CTNAG	9 cut(s)	131, 509, 1162, 1527, 1565, 1875, 2388, 2511, 2543
HpySE526I	ACGT	2 cut(s)	398, 1543
Hsp92I	GRCGYC	2 cut(s)	102, 505
HspAI	GCGC	1 cut(s)	102
KasI	GGCGCC	1 cut(s)	101
Ksp22I	TGATCA	1 cut(s)	2596
LmnI	GCTCC	4 cut(s)	498, 1727, 1939, 2558
Lsp1109I	GCAGC	9 cut(s)	49, 72, 656, 916, 1240, 1592, 1666, 1858, 2311
LweI	GCATC	6 cut(s)	614, 793, 972, 1006, 1618, 1691
MaeII	ACGT	2 cut(s)	398, 1543
MaeIII	GTNAC	9 cut(s)	595, 650, 656, 727, 1243, 1853, 1927, 2422, 2493
MflI	RGATCY	3 cut(s)	1495, 1555, 1633
MhlI	GDGCHC	4 cut(s)	526, 1732, 2065, 2124
MlsI	TGGCCA	1 cut(s)	2619
MluNI	TGGCCA	1 cut(s)	2619
Mly113I	GGCGCC	1 cut(s)	102
MlyI	GAGTC	5 cut(s)	132, 1020, 1036, 1393, 1742
MmeI	TCCRAC	1 cut(s)	1869
Mox20I	TGGCCA	1 cut(s)	2619
MroXI	GAANNNNTTC	2 cut(s)	696, 1908
MscI	TGGCCA	1 cut(s)	2619
MseI	TTAA	2 cut(s)	243, 1418
Msp20I	TGGCCA	1 cut(s)	2619
MspA1I	CMGCKG	1 cut(s)	2324
MspCI	CTTAAG	1 cut(s)	1417
MspI	CCGG	6 cut(s)	81, 136, 160, 469, 1396, 2531
MspR9I	CCNGG	4 cut(s)	106, 335, 2166, 2222
MvaI	CCWGG	4 cut(s)	106, 335, 2166, 2222
MvnI	CGCG	1 cut(s)	2057
MwoI	GCNNNNNNNGC	2 cut(s)	1396, 1868
NarI	GGCGCC	1 cut(s)	102
NcoI	CCATGG	2 cut(s)	886, 2044
NdeI	CATATG	1 cut(s)	475
NheI	GCTAGC	1 cut(s)	1537
NlaIV	GGNNCC	7 cut(s)	103, 258, 892, 953, 1497, 1635, 1951
NmeAIII	GCCGAG	2 cut(s)	78, 1201
NmuCI	GTSAC	7 cut(s)	595, 656, 727, 1243, 1853, 1927, 2422
NspI	RCATGY	3 cut(s)	493, 829, 1537
NspV	TTCGAA	1 cut(s)	228
PaeR7I	CTCGAG	1 cut(s)	2498
PagI	TCATGA	1 cut(s)	1792
PcsI	WCGNNNNNNNCGW	1 cut(s)	1974
PdmI	GAANNNNTTC	2 cut(s)	696, 1908
PfeI	GAWTC	4 cut(s)	718, 1049, 1643, 1796
PleI	GAGTC	5 cut(s)	131, 1020, 1036, 1392, 1742
PluTI	GGCGCC	1 cut(s)	105
PmaCI	CACGTG	1 cut(s)	1544
PmlI	CACGTG	1 cut(s)	1544
PpsI	GAGTC	5 cut(s)	131, 1020, 1036, 1392, 1742
Ppu21I	YACGTR	2 cut(s)	399, 1544
Psp124BI	GAGCTC	1 cut(s)	2065
Psp6I	CCWGG	4 cut(s)	104, 333, 2164, 2220
PspCI	CACGTG	1 cut(s)	1544
PspGI	CCWGG	4 cut(s)	104, 333, 2164, 2220
PspN4I	GGNNCC	7 cut(s)	103, 258, 892, 953, 1497, 1635, 1951
PspPI	GGNCC	4 cut(s)	257, 891, 1371, 2052
PspXI	VCTCGAGB	1 cut(s)	2498
PsrI	GAACNNNNNNTAC	2 cut(s)	545, 577
PstI	CTGCAG	1 cut(s)	975
PsuI	RGATCY	3 cut(s)	1495, 1555, 1633
PvuII	CAGCTG	1 cut(s)	2324
RsaI	GTAC	7 cut(s)	389, 397, 1118, 1181, 1338, 1764, 2641
RsaNI	GTAC	7 cut(s)	388, 396, 1117, 1180, 1337, 1763, 2640
SacI	GAGCTC	1 cut(s)	2065
SaqAI	TTAA	2 cut(s)	243, 1418
Sau96I	GGNCC	4 cut(s)	257, 891, 1371, 2052
SbfI	CCTGCAGG	1 cut(s)	975
ScaI	AGTACT	1 cut(s)	1181
SchI	GAGTC	5 cut(s)	132, 1020, 1036, 1393, 1742
ScrFI	CCNGG	4 cut(s)	106, 335, 2166, 2222
SdaI	CCTGCAGG	1 cut(s)	975
SduI	GDGCHC	4 cut(s)	526, 1732, 2065, 2124
SfaNI	GCATC	6 cut(s)	614, 793, 972, 1006, 1618, 1691
SfcI	CTRYAG	3 cut(s)	67, 300, 971
SfoI	GGCGCC	1 cut(s)	103
Sfr274I	CTCGAG	1 cut(s)	2498
SfuI	TTCGAA	1 cut(s)	228
SinI	GGWCC	4 cut(s)	257, 891, 1371, 2052
SlaI	CTCGAG	1 cut(s)	2498
SmlI	CTYRAG	5 cut(s)	1072, 1271, 1417, 2498, 2631
SmoI	CTYRAG	5 cut(s)	1072, 1271, 1417, 2498, 2631
Sse8387I	CCTGCAGG	1 cut(s)	975
SsiI	CCGC	4 cut(s)	27, 1256, 2055, 2269
SspDI	GGCGCC	1 cut(s)	101
SspI	AATATT	2 cut(s)	280, 1206
SstI	GAGCTC	1 cut(s)	2065
StyD4I	CCNGG	4 cut(s)	104, 333, 2164, 2220
StyI	CCWWGG	6 cut(s)	838, 886, 1687, 2044, 2179, 2273
TaiI	ACGT	2 cut(s)	401, 1546
TaqI	TCGA	8 cut(s)	228, 721, 898, 1107, 1799, 2263, 2376, 2499
TatI	WGTACW	3 cut(s)	1116, 1179, 2639
TauI	GCSGC	2 cut(s)	30, 2271
TfiI	GAWTC	4 cut(s)	718, 1049, 1643, 1796
Tru1I	TTAA	2 cut(s)	243, 1418
Tru9I	TTAA	2 cut(s)	243, 1418
TscAI	CASTG	7 cut(s)	214, 620, 741, 1252, 1873, 1977, 2620
TseFI	GTSAC	7 cut(s)	595, 656, 727, 1243, 1853, 1927, 2422
TseI	GCWGC	9 cut(s)	37, 60, 644, 929, 1253, 1580, 1679, 1871, 2324
Tsp45I	GTSAC	7 cut(s)	595, 656, 727, 1243, 1853, 1927, 2422
TspGWI	ACGGA	2 cut(s)	485, 789
TspRI	CASTG	7 cut(s)	214, 620, 741, 1252, 1873, 1977, 2620
Vha464I	CTTAAG	1 cut(s)	1417
VpaK11BI	GGWCC	4 cut(s)	257, 891, 1371, 2052
XagI	CCTNNNNNAGG	2 cut(s)	338, 2362
XapI	RAATTY	6 cut(s)	171, 230, 908, 2000, 2169, 2516
XceI	RCATGY	3 cut(s)	493, 829, 1537
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1813
XhoI	CTCGAG	1 cut(s)	2498
XmaJI	CCTAGG	1 cut(s)	2179
XmiI	GTMKAC	1 cut(s)	1549
XmnI	GAANNNNTTC	2 cut(s)	696, 1908
ZrmI	AGTACT	1 cut(s)	1181

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.