Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0006469

Physical Location & Seq

Genomic Coordinates Reverse (-)

3365208 .. 3371440

Gene Structure 6233 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G003680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2544 bp

ATGGCGTTTACTGGTGTTTCAATGTGCGGCCAAGAGCAGCTAGACTTGTCCAGAGACGGCAGCCACTACAGTCTCTCCACCGGAATACTGCCGTCGCTCGGCGCCAGGTCTAGTAATCGGAGAGTCAAGCTCAGCCGGTTTATTGTTTCGCCTTACGACCGGCGATACAGAATTTGGGAGACATTTCTTGTTGTTCTGGTCATCTACACTGCTTGGGTCTCCCCCTTCGAATTTGGCTTTCTTAACAAACCACAGGGACCACTCTCCATTATTGACAATATTGTCAATGGCTTCTTTGCTATAGATATTGTTCTTACATTCTTTGTGGCTTACCTGGATAAGGCTACATATCTACTTGTTGACGATCAAAAGAAGATTGCTTGGAAGTACGCAAGTACGTGGTTGATCTTTGATGTCATATCCACAATTCCTACTGAACTTGCTAGGAAGATTTCCCCTAAACCTTTCCGGTCATATGGCTTTTTCAACATGCTCCGTCTTTGGCGTCTTAGAAGAGTTAGTGCTCTATTTTCCAGATTGGAGAAAGATAGGAACTATAATTACTTTTGGGTTCGTTGTGCAAAACTTATTTGTGTCACCCTGTTTGCGGTTCACTGTGCTGGATGCTTCTATTACCTTCTTGCAGCCCGTTACCGTGACCCCAAGAACACATGGATTGGAAAAACAATGGAAGATTTTCTTCATCAAAGTCTCTGGATTCGATATGTGACTTCAGTTTATTGGTCTATCACTACACTCACAACCGTTGGCTACGGAGATTTGCATCCTGTCAATACAAGGGAGATGATCTTTGACATATTCTACATGCTTTTCAACCTTGGCTTGACATCATACTTGATTGGAAATATGACCAACTTGGTTGTCCATGGGACCAGTCGAACACGAAAATTTAGGGATACCATACAAGCTGCCTCCAGTTTTGCACAAAGGAACCAACTGCCTGTTCGCCTGCAGGATCAGATGCTTGCCCACTTGTGTCTGAAGTTCAGGACAGATGCAGAGGGACTCCAGCAACAAGAGACTCTTGATTCACTTCCGAAAGCCATACGCTCAAGTATTTCACATTATCTGTTTTACTCTCTTGTCGATAAGGTGTACTTGTTTCATGGGGTTTCAAATGACTTGCTTTTTCAGTTGGTCTCAGAGATGAAAGCTGAGTATTTTCCTCCCAAAGAAGATGTAATATTGCAGAATGAAGCACCTACAGATTTCTACATACTTGTCACTGGTGCTGCGGATTTAGTGGTCCTCAAGAATGGAGTTGAACAGGTCATTGGTGAGGCAAAAACTGGTGATCTTATTGGTGAAATTGGGGTACTCTGTTATAGGCCACAGCTTTTTACCGTTAGGACCAAAAGATTGAGTCAGCTACTCCGGCTGAACCGAACTACATTCTTAAGCATAGTTCAAGCTAATGTTGGGGATGGAACTATAATAATGAATAACCTCCTCCAGCATTTGAAAGAGGTCAAGGATCCATATATGGAGGGAGTTTTGCTGGAGACTGAGAACATGCTAGCACGTGGTAGACTGGATCTACCTCTCAGTCTATGCTTTGCAGCAATTAGAGGAGATGACTTGCTGTTGCATCAGTTGTTGAGACGGGGTTTGGATCCGAATGAATCAGACAATAATGGCAGAACAGCTCTGCATATAGCTGCATCCCAAGGAAGTGAGAACTGTGTGCTTCTACTATTGGACTATGGAGCACTTCCTAACAGTAGAGACTCGGAGGGAAGTGTACCACTTTGGGAGGCAATACAGAAGGATCATGAATCGATTGCCAAACTGCTGATGGACAACGGTGCAACTTTGAATGCTGGGGATGTTGGTCACTTTGCTTGCACTGCTGCTGAGCAAAACAGGTTGGACTTGCTCAATGAAATAGTTCAACATGGAGGAGATGTCACAGCTCCTAGAAGCAATGGAACCACAGCTCTCCATGTCGCAGTGTCCGAAGACAACATTGAAATTGTGAAATTCCTTTTGGACAAAGGGGCAGATATTGACAAACCAGACCTCCATGGCTGGACCGCGAGAGCTCTAGCCGACCAACAAGGACATGAAGAGATAAAGAACCTTTTTCAATCTAGAAAAGAGCCCGAAGCTCAATCTGTTATCACCATTCCTACTGTGCAAAAGCCTGGAATTCGCTTCCTTGGGAGATTTACAAGCGAGCCAAACATCCATCCTCCTTCCCAGGAAGGCTCATTTCTAGTAGCAGATGGGGGATCATGGGGTCGAAGCCGCCCAAGGCGTAGGACTAACAACTTCCACAACTCACTTTTTGGGATGATGTCAGCTGCACACACAGGGGAGAAGGATTTGTTCTTCTCCTCAATTAGGGAGACCGTCGAAACCACTCCTAAGAGTATTGGGAGTAATCCTAACCCTGCTAGAGTGACAATTAGTTGCCCTGAAAATAGTGAAGTTTCAGGGAAACTTGTATTACTTCCTGCAGTTTTGAGGAGTTACTCGAGGTTGGTGCTAAGAAATTCGGTTTCACTCGGGCTAAAGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008324 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010035 GO:0010053 GO:0010054 GO:0010107 GO:0010119 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016049 GO:0021700 GO:0022622 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0034220 GO:0040007 GO:0042221 GO:0042391 GO:0042802 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071804 GO:0071805 GO:0071944 GO:0080147 GO:0090333 GO:0090558 GO:0090627 GO:0098655 GO:0098660 GO:0098662 GO:0099094 GO:0099402 GO:1901700 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

847

Amino Acids

95.46

Weight (kDa)

8.45

Isoelectric Point (pI)

40.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	56 - 301	2.8e-44	Ion transport protein
Ion_trans_2	PF07885	204 - 294	3.5e-13	Ion channel
cNMP_binding	PF00027	394 - 479	2.4e-14	Cyclic nucleotide-binding domain
Ank_2	PF12796	524 - 615	4.8e-17	Ankyrin repeats (3 copies)
Ank_4	PF13637	524 - 573	6.8e-09	Ankyrin repeats (many copies)
Ank_5	PF13857	539 - 590	3.4e-08	Ankyrin repeats (many copies)
Ank	PF00023	552 - 583	3.9e-07	Ankyrin repeat
Ank_2	PF12796	623 - 701	1.4e-11	Ankyrin repeats (3 copies)
Ank_4	PF13637	623 - 670	1.1e-07	Ankyrin repeats (many copies)
Ank_5	PF13857	637 - 690	9.1e-08	Ankyrin repeats (many copies)
Ank	PF00023	649 - 680	3.6e-08	Ankyrin repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006469)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26650 AT2G26650
fragaria_vesca	FvH4_1g03960 FvH4_1g03960 FvH4_1g03960 FvH4_1g03960 FvH4_1g03960 FvH4_3g32370
malus_domestica	MD02G1037900.v1.1 MD15G1178200.v1.1
prunus_persica	Prupe.7G237400_v2.0.a1 Prupe.7G237400_v2.0.a1 Prupe.7G237400_v2.0.a1
pyrus_communis	pycom15g15990
rosa_chinensis	RchiOBHm_Chr2g0089421
rosa_laevigata	RLG00000016026
rosa_multiflora	Rmu_sc0000197.1_g000013
rosa_roxburghii	Rroxscaffold_2G00151950
rosa_rugosa	Rorug02G0000500 Rorug02G0000600 Rorug02G0000700
rosa_samantha	Rh2AG045800 Rh2BG044300 Rh2CG046200 Rh2DG045700
rosa_wichuraiana	Rw2G003680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	281
AccB1I	GGYRCC	1 cut(s)	101
AccI	GTMKAC	1 cut(s)	1549
AccII	CGCG	1 cut(s)	2057
AciI	CCGC	5 cut(s)	27, 608, 1256, 2055, 2269
AclWI	GGATC	8 cut(s)	984, 1490, 1503, 1563, 1628, 1641, 1797, 2260
AcoI	YGGCCR	1 cut(s)	28
AcsI	RAATTY	6 cut(s)	171, 230, 908, 2000, 2169, 2515
AcuI	CTGAAG	2 cut(s)	717, 1022
AcvI	CACGTG	1 cut(s)	1544
AcyI	GRCGYC	2 cut(s)	102, 505
AfaI	GTAC	5 cut(s)	389, 397, 1118, 1338, 1764
AfiI	CCNNNNNNNGG	4 cut(s)	98, 340, 607, 2364
AflII	CTTAAG	1 cut(s)	1417
AjnI	CCWGG	4 cut(s)	104, 333, 2164, 2220
AjuI	GAANNNNNNNTTGG	6 cut(s)	948, 980, 1278, 1310, 1991, 2023
Alw21I	GWGCWC	3 cut(s)	526, 1732, 2065
AlwI	GGATC	8 cut(s)	984, 1490, 1503, 1563, 1628, 1641, 1797, 2260
Ama87I	CYCGRG	2 cut(s)	2497, 2528
AoxI	GGCC	2 cut(s)	28, 1349
ApeKI	GCWGC	9 cut(s)	37, 60, 644, 929, 1253, 1580, 1679, 1871, 2324
ApoI	RAATTY	6 cut(s)	171, 230, 908, 2000, 2169, 2515
Asp700I	GAANNNNTTC	2 cut(s)	696, 1908
AspLEI	GCGC	1 cut(s)	104
AspS9I	GGNCC	5 cut(s)	257, 891, 1267, 1371, 2052
AsuHPI	GGTGA	5 cut(s)	589, 1310, 1325, 1337, 2134
AsuII	TTCGAA	1 cut(s)	228
AsuNHI	GCTAGC	1 cut(s)	1537
AvaI	CYCGRG	2 cut(s)	2497, 2528
AvaII	GGWCC	5 cut(s)	257, 891, 1267, 1371, 2052
BamHI	GGATCC	2 cut(s)	1495, 1633
BanI	GGYRCC	1 cut(s)	101
BanII	GRGCYC	2 cut(s)	2065, 2124
BbrPI	CACGTG	1 cut(s)	1544
BbsI	GAAGAC	1 cut(s)	1986
Bbv12I	GWGCWC	3 cut(s)	526, 1732, 2065
BbvI	GCAGC	9 cut(s)	49, 72, 656, 916, 1240, 1592, 1666, 1858, 2311
BccI	CCATC	4 cut(s)	1439, 1810, 2217, 2240
BceAI	ACGGC	2 cut(s)	73, 76
BcgI	CGANNNNNNTGC	2 cut(s)	2488, 2522
BciT130I	CCWGG	4 cut(s)	106, 335, 2166, 2222
BciVI	GTATCC	1 cut(s)	910
BfaI	CTAG	9 cut(s)	41, 111, 444, 1538, 1938, 2066, 2112, 2237, 2418
BfmI	CTRYAG	5 cut(s)	67, 300, 971, 1224, 2478
BfoI	RGCGCY	1 cut(s)	105
BfrI	CTTAAG	1 cut(s)	1417
BfuI	GTATCC	1 cut(s)	910
BlpI	GCTNAGC	2 cut(s)	131, 1875
Bme1390I	CCNGG	4 cut(s)	106, 335, 2166, 2222
Bme18I	GGWCC	5 cut(s)	257, 891, 1267, 1371, 2052
BmeT110I	CYCGRG	2 cut(s)	2497, 2528
BmgT120I	GGNCC	5 cut(s)	257, 891, 1267, 1371, 2052
BmiI	GGNNCC	7 cut(s)	103, 258, 892, 953, 1497, 1635, 1951
BmrFI	CCNGG	4 cut(s)	106, 335, 2166, 2222
BmsI	GCATC	6 cut(s)	614, 793, 972, 1006, 1618, 1691
BmtI	GCTAGC	1 cut(s)	1541
BpiI	GAAGAC	1 cut(s)	1986
BplI	GAGNNNNNCTC	2 cut(s)	114, 146
BpmI	CTGGAG	4 cut(s)	919, 1013, 1457, 1541
Bpu1102I	GCTNAGC	2 cut(s)	131, 1875
Bpu14I	TTCGAA	1 cut(s)	228
BpuEI	CTTGAG	2 cut(s)	1057, 1256
Bsa29I	ATCGAT	1 cut(s)	1799
BsaAI	YACGTR	2 cut(s)	399, 1544
BsaBI	GATNNNNATC	1 cut(s)	783
BsaHI	GRCGYC	2 cut(s)	102, 505
BsaI	GGTCTC	3 cut(s)	223, 1165, 2363
BsaJI	CCNNGG	7 cut(s)	838, 886, 1687, 2044, 2179, 2220, 2273
BsaWI	WCCGGW	2 cut(s)	80, 468
BsaXI	ACNNNNNCTCC	6 cut(s)	59, 89, 1911, 1941, 2025, 2055
Bsc4I	CCNNNNNNNGG	4 cut(s)	98, 340, 607, 2364
Bse118I	RCCGGY	2 cut(s)	135, 159
Bse1I	ACTGG	6 cut(s)	16, 894, 936, 1252, 1315, 1557
Bse3DI	GCAATG	1 cut(s)	1951
Bse8I	GATNNNNATC	1 cut(s)	783
BseBI	CCWGG	4 cut(s)	106, 335, 2166, 2222
BseCI	ATCGAT	1 cut(s)	1799
BseDI	CCNNGG	7 cut(s)	838, 886, 1687, 2044, 2179, 2220, 2273
BseGI	GGATG	8 cut(s)	629, 784, 1450, 1682, 1852, 2205, 2209, 2319
BseJI	GATNNNNATC	1 cut(s)	783
BseLI	CCNNNNNNNGG	4 cut(s)	98, 340, 607, 2364
BseMI	GCAATG	1 cut(s)	1951
BseMII	CTCAG	6 cut(s)	145, 1167, 1176, 1518, 1579, 1866
BseNI	ACTGG	6 cut(s)	16, 894, 936, 1252, 1315, 1557
BseRI	GAGGAG	5 cut(s)	1460, 1605, 1935, 2347, 2503
BseXI	GCAGC	9 cut(s)	49, 72, 656, 916, 1240, 1592, 1666, 1858, 2311
BseYI	CCCAGC	1 cut(s)	1841
BsgI	GTGCAG	1 cut(s)	2310
Bsh1236I	CGCG	1 cut(s)	2057
Bsh1285I	CGRYCG	1 cut(s)	160
BshFI	GGCC	2 cut(s)	30, 1351
BshNI	GGYRCC	1 cut(s)	101
BshVI	ATCGAT	1 cut(s)	1799
BsiEI	CGRYCG	1 cut(s)	160
BsiHKAI	GWGCWC	3 cut(s)	526, 1732, 2065
BsiHKCI	CYCGRG	2 cut(s)	2497, 2528
BsiSI	CCGG	5 cut(s)	81, 136, 160, 469, 1396
BslFI	GGGAC	3 cut(s)	270, 904, 1038
BslI	CCNNNNNNNGG	4 cut(s)	98, 340, 607, 2364
BsmBI	CGTCTC	2 cut(s)	48, 1615
BsmFI	GGGAC	3 cut(s)	270, 904, 1038
BsmI	GAATGC	1 cut(s)	1843
BsnI	GGCC	2 cut(s)	30, 1351
Bso31I	GGTCTC	3 cut(s)	223, 1165, 2363
BsoBI	CYCGRG	2 cut(s)	2497, 2528
Bsp119I	TTCGAA	1 cut(s)	228
Bsp1286I	GDGCHC	4 cut(s)	526, 1732, 2065, 2124
Bsp1720I	GCTNAGC	2 cut(s)	131, 1875
Bsp19I	CCATGG	2 cut(s)	886, 2044
BspACI	CCGC	5 cut(s)	27, 608, 1256, 2055, 2269
BspANI	GGCC	2 cut(s)	30, 1351
BspCNI	CTCAG	6 cut(s)	144, 1168, 1175, 1519, 1578, 1867
BspDI	ATCGAT	1 cut(s)	1799
BspFNI	CGCG	1 cut(s)	2057
BspHI	TCATGA	1 cut(s)	1792
BspLI	GGNNCC	7 cut(s)	103, 258, 892, 953, 1497, 1635, 1951
BspMAI	CTGCAG	2 cut(s)	975, 2482
BspOI	GCTAGC	1 cut(s)	1541
BspPI	GGATC	8 cut(s)	984, 1490, 1503, 1563, 1628, 1641, 1797, 2260
BspT104I	TTCGAA	1 cut(s)	228
BspT107I	GGYRCC	1 cut(s)	101
BspTI	CTTAAG	1 cut(s)	1417
BspTNI	GGTCTC	3 cut(s)	223, 1165, 2363
BsrDI	GCAATG	1 cut(s)	1951
BsrFI	RCCGGY	2 cut(s)	135, 159
BsrI	ACTGG	6 cut(s)	16, 894, 936, 1252, 1315, 1557
BssAI	RCCGGY	2 cut(s)	135, 159
BssECI	CCNNGG	7 cut(s)	838, 886, 1687, 2044, 2179, 2220, 2273
BssNI	GRCGYC	2 cut(s)	102, 505
BssT1I	CCWWGG	6 cut(s)	838, 886, 1687, 2044, 2179, 2273
Bst2UI	CCWGG	4 cut(s)	106, 335, 2166, 2222
Bst6I	CTCTTC	2 cut(s)	508, 2082
BstACI	GRCGYC	2 cut(s)	102, 505
BstAFI	CTTAAG	1 cut(s)	1417
BstBAI	YACGTR	2 cut(s)	399, 1544
BstBI	TTCGAA	1 cut(s)	228
BstC8I	GCNNGC	5 cut(s)	971, 987, 1539, 1864, 2198
BstDEI	CTNAG	9 cut(s)	131, 509, 1162, 1176, 1527, 1565, 1875, 2388, 2510
BstDSI	CCRYGG	2 cut(s)	886, 2044
BstENI	CCTNNNNNAGG	2 cut(s)	338, 2362
BstF5I	GGATG	8 cut(s)	629, 784, 1450, 1682, 1852, 2205, 2209, 2319
BstFNI	CGCG	1 cut(s)	2057
BstH2I	RGCGCY	1 cut(s)	105
BstHHI	GCGC	1 cut(s)	104
BstMCI	CGRYCG	1 cut(s)	160
BstMWI	GCNNNNNNNGC	2 cut(s)	1396, 1868
BstNI	CCWGG	4 cut(s)	106, 335, 2166, 2222
BstNSI	RCATGY	3 cut(s)	493, 829, 1537
BstSCI	CCNGG	4 cut(s)	104, 333, 2164, 2220
BstSFI	CTRYAG	5 cut(s)	67, 300, 971, 1224, 2478
BstUI	CGCG	1 cut(s)	2057
BstV1I	GCAGC	9 cut(s)	49, 72, 656, 916, 1240, 1592, 1666, 1858, 2311
BstV2I	GAAGAC	1 cut(s)	1986
BstX2I	RGATCY	3 cut(s)	1495, 1555, 1633
BstYI	RGATCY	3 cut(s)	1495, 1555, 1633
Bsu15I	ATCGAT	1 cut(s)	1799
BsuI	GTATCC	1 cut(s)	910
BsuRI	GGCC	2 cut(s)	30, 1351
BsuTUI	ATCGAT	1 cut(s)	1799
BtgI	CCRYGG	2 cut(s)	886, 2044
BtsCI	GGATG	8 cut(s)	629, 784, 1450, 1682, 1852, 2205, 2209, 2319
BtsI	GCAGTG	3 cut(s)	207, 1866, 1977
BtsIMutI	CAGTG	5 cut(s)	207, 613, 1245, 1866, 1977
Cac8I	GCNNGC	5 cut(s)	971, 987, 1539, 1864, 2198
CciI	TCATGA	1 cut(s)	1792
CfoI	GCGC	1 cut(s)	104
Cfr10I	RCCGGY	2 cut(s)	135, 159
Cfr13I	GGNCC	5 cut(s)	257, 891, 1267, 1371, 2052
ClaI	ATCGAT	1 cut(s)	1799
CseI	GACGC	1 cut(s)	494
Csp6I	GTAC	5 cut(s)	388, 396, 1117, 1337, 1763
CviQI	GTAC	5 cut(s)	388, 396, 1117, 1337, 1763
DdeI	CTNAG	9 cut(s)	131, 509, 1162, 1176, 1527, 1565, 1875, 2388, 2510
DinI	GGCGCC	1 cut(s)	103
DrdI	GACNNNNNNGTC	1 cut(s)	281
DseDI	GACNNNNNNGTC	1 cut(s)	281
EaeI	YGGCCR	1 cut(s)	28
Eam1104I	CTCTTC	2 cut(s)	508, 2082
EarI	CTCTTC	2 cut(s)	508, 2082
Ecl136II	GAGCTC	1 cut(s)	2063
Eco130I	CCWWGG	6 cut(s)	838, 886, 1687, 2044, 2179, 2273
Eco24I	GRGCYC	2 cut(s)	2065, 2124
Eco31I	GGTCTC	3 cut(s)	223, 1165, 2363
Eco47I	GGWCC	5 cut(s)	257, 891, 1267, 1371, 2052
Eco53kI	GAGCTC	1 cut(s)	2063
Eco57I	CTGAAG	2 cut(s)	717, 1022
Eco72I	CACGTG	1 cut(s)	1544
Eco88I	CYCGRG	2 cut(s)	2497, 2528
EcoICRI	GAGCTC	1 cut(s)	2063
EcoNI	CCTNNNNNAGG	2 cut(s)	338, 2362
EcoRI	GAATTC	1 cut(s)	2169
EcoRII	CCWGG	4 cut(s)	104, 333, 2164, 2220
EcoT14I	CCWWGG	6 cut(s)	838, 886, 1687, 2044, 2179, 2273
EcoT38I	GRGCYC	2 cut(s)	2065, 2124
EgeI	GGCGCC	1 cut(s)	103
EheI	GGCGCC	1 cut(s)	103
ErhI	CCWWGG	6 cut(s)	838, 886, 1687, 2044, 2179, 2273
Esp3I	CGTCTC	2 cut(s)	48, 1615
FalI	AAGNNNNNCTT	6 cut(s)	684, 716, 1029, 1061, 1103, 1135
FaqI	GGGAC	3 cut(s)	270, 904, 1038
FauNDI	CATATG	1 cut(s)	475
FblI	GTMKAC	1 cut(s)	1549
FokI	GGATG	8 cut(s)	636, 771, 1457, 1669, 1859, 2192, 2196, 2326
FriOI	GRGCYC	2 cut(s)	2065, 2124
FspBI	CTAG	9 cut(s)	41, 111, 444, 1538, 1938, 2066, 2112, 2237, 2418
GlaI	GCGC	1 cut(s)	103
GsaI	CCCAGC	1 cut(s)	1845
GsuI	CTGGAG	4 cut(s)	919, 1013, 1457, 1541
HaeII	RGCGCY	1 cut(s)	105
HaeIII	GGCC	2 cut(s)	30, 1351
HapII	CCGG	5 cut(s)	81, 136, 160, 469, 1396
HgaI	GACGC	1 cut(s)	494
HhaI	GCGC	1 cut(s)	104
Hin1I	GRCGYC	2 cut(s)	102, 505
Hin6I	GCGC	1 cut(s)	102
HinP1I	GCGC	1 cut(s)	102
HincII	GTYRAC	1 cut(s)	361
HindII	GTYRAC	1 cut(s)	361
HinfI	GANTC	9 cut(s)	123, 718, 1026, 1042, 1049, 1384, 1643, 1748, 1796
HpaII	CCGG	5 cut(s)	81, 136, 160, 469, 1396
HphI	GGTGA	5 cut(s)	589, 1310, 1325, 1337, 2134
Hpy166II	GTNNAC	6 cut(s)	9, 361, 613, 1117, 1550, 1763
Hpy188III	TCNNGA	8 cut(s)	51, 534, 715, 1009, 1046, 1273, 1793, 2112
Hpy8I	GTNNAC	6 cut(s)	9, 361, 613, 1117, 1550, 1763
Hpy99I	CGWCG	2 cut(s)	97, 2378
HpyAV	CCTTC	6 cut(s)	235, 647, 1780, 2219, 2226, 2335
HpyCH4IV	ACGT	2 cut(s)	398, 1543
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1396, 1868
HpyF3I	CTNAG	9 cut(s)	131, 509, 1162, 1176, 1527, 1565, 1875, 2388, 2510
HpySE526I	ACGT	2 cut(s)	398, 1543
Hsp92I	GRCGYC	2 cut(s)	102, 505
HspAI	GCGC	1 cut(s)	102
KasI	GGCGCC	1 cut(s)	101
LmnI	GCTCC	3 cut(s)	498, 1727, 1939
Lsp1109I	GCAGC	9 cut(s)	49, 72, 656, 916, 1240, 1592, 1666, 1858, 2311
LweI	GCATC	6 cut(s)	614, 793, 972, 1006, 1618, 1691
MaeI	CTAG	9 cut(s)	41, 111, 444, 1538, 1938, 2066, 2112, 2237, 2418
MaeII	ACGT	2 cut(s)	398, 1543
MaeIII	GTNAC	9 cut(s)	595, 650, 656, 727, 1243, 1853, 1927, 2422, 2492
MboII	GAAGA	9 cut(s)	385, 460, 525, 692, 704, 1208, 1991, 2099, 2344
MflI	RGATCY	3 cut(s)	1495, 1555, 1633
MhlI	GDGCHC	4 cut(s)	526, 1732, 2065, 2124
Mly113I	GGCGCC	1 cut(s)	102
MlyI	GAGTC	5 cut(s)	132, 1020, 1036, 1393, 1742
MmeI	TCCRAC	1 cut(s)	1869
MroXI	GAANNNNTTC	2 cut(s)	696, 1908
MseI	TTAA	2 cut(s)	243, 1418
MspA1I	CMGCKG	1 cut(s)	2324
MspCI	CTTAAG	1 cut(s)	1417
MspI	CCGG	5 cut(s)	81, 136, 160, 469, 1396
MspR9I	CCNGG	4 cut(s)	106, 335, 2166, 2222
Mva1269I	GAATGC	1 cut(s)	1843
MvaI	CCWGG	4 cut(s)	106, 335, 2166, 2222
MvnI	CGCG	1 cut(s)	2057
MwoI	GCNNNNNNNGC	2 cut(s)	1396, 1868
NarI	GGCGCC	1 cut(s)	102
NcoI	CCATGG	2 cut(s)	886, 2044
NdeI	CATATG	1 cut(s)	475
NheI	GCTAGC	1 cut(s)	1537
NlaIV	GGNNCC	7 cut(s)	103, 258, 892, 953, 1497, 1635, 1951
NmeAIII	GCCGAG	1 cut(s)	78
NmuCI	GTSAC	7 cut(s)	595, 656, 727, 1243, 1853, 1927, 2422
NspI	RCATGY	3 cut(s)	493, 829, 1537
NspV	TTCGAA	1 cut(s)	228
PaeR7I	CTCGAG	1 cut(s)	2497
PagI	TCATGA	1 cut(s)	1792
PcsI	WCGNNNNNNNCGW	1 cut(s)	1974
PctI	GAATGC	1 cut(s)	1843
PdmI	GAANNNNTTC	2 cut(s)	696, 1908
PfeI	GAWTC	4 cut(s)	718, 1049, 1643, 1796
PleI	GAGTC	5 cut(s)	131, 1020, 1036, 1392, 1742
PluTI	GGCGCC	1 cut(s)	105
PmaCI	CACGTG	1 cut(s)	1544
PmlI	CACGTG	1 cut(s)	1544
PpsI	GAGTC	5 cut(s)	131, 1020, 1036, 1392, 1742
Ppu21I	YACGTR	2 cut(s)	399, 1544
Psp124BI	GAGCTC	1 cut(s)	2065
Psp6I	CCWGG	4 cut(s)	104, 333, 2164, 2220
PspCI	CACGTG	1 cut(s)	1544
PspFI	CCCAGC	1 cut(s)	1841
PspGI	CCWGG	4 cut(s)	104, 333, 2164, 2220
PspN4I	GGNNCC	7 cut(s)	103, 258, 892, 953, 1497, 1635, 1951
PspPI	GGNCC	5 cut(s)	257, 891, 1267, 1371, 2052
PspXI	VCTCGAGB	1 cut(s)	2497
PsrI	GAACNNNNNNTAC	2 cut(s)	545, 577
PstI	CTGCAG	2 cut(s)	975, 2482
PsuI	RGATCY	3 cut(s)	1495, 1555, 1633
PvuII	CAGCTG	1 cut(s)	2324
RsaI	GTAC	5 cut(s)	389, 397, 1118, 1338, 1764
RsaNI	GTAC	5 cut(s)	388, 396, 1117, 1337, 1763
SacI	GAGCTC	1 cut(s)	2065
SaqAI	TTAA	2 cut(s)	243, 1418
Sau96I	GGNCC	5 cut(s)	257, 891, 1267, 1371, 2052
SbfI	CCTGCAGG	1 cut(s)	975
SchI	GAGTC	5 cut(s)	132, 1020, 1036, 1393, 1742
ScrFI	CCNGG	4 cut(s)	106, 335, 2166, 2222
SdaI	CCTGCAGG	1 cut(s)	975
SduI	GDGCHC	4 cut(s)	526, 1732, 2065, 2124
SfaNI	GCATC	6 cut(s)	614, 793, 972, 1006, 1618, 1691
SfcI	CTRYAG	5 cut(s)	67, 300, 971, 1224, 2478
SfoI	GGCGCC	1 cut(s)	103
Sfr274I	CTCGAG	1 cut(s)	2497
SfuI	TTCGAA	1 cut(s)	228
SinI	GGWCC	5 cut(s)	257, 891, 1267, 1371, 2052
SlaI	CTCGAG	1 cut(s)	2497
SmlI	CTYRAG	4 cut(s)	1072, 1271, 1417, 2497
SmoI	CTYRAG	4 cut(s)	1072, 1271, 1417, 2497
Sse8387I	CCTGCAGG	1 cut(s)	975
SsiI	CCGC	5 cut(s)	27, 608, 1256, 2055, 2269
SspDI	GGCGCC	1 cut(s)	101
SspI	AATATT	2 cut(s)	280, 1206
SspMI	CTAG	9 cut(s)	41, 111, 444, 1538, 1938, 2066, 2112, 2237, 2418
SstI	GAGCTC	1 cut(s)	2065
StyD4I	CCNGG	4 cut(s)	104, 333, 2164, 2220
StyI	CCWWGG	6 cut(s)	838, 886, 1687, 2044, 2179, 2273
TaiI	ACGT	2 cut(s)	401, 1546
TaqI	TCGA	8 cut(s)	228, 721, 898, 1107, 1799, 2263, 2376, 2498
TatI	WGTACW	1 cut(s)	1116
TauI	GCSGC	2 cut(s)	30, 2271
TfiI	GAWTC	4 cut(s)	718, 1049, 1643, 1796
Tru1I	TTAA	2 cut(s)	243, 1418
Tru9I	TTAA	2 cut(s)	243, 1418
TscAI	CASTG	5 cut(s)	214, 620, 1252, 1873, 1977
TseFI	GTSAC	7 cut(s)	595, 656, 727, 1243, 1853, 1927, 2422
TseI	GCWGC	9 cut(s)	37, 60, 644, 929, 1253, 1580, 1679, 1871, 2324
Tsp45I	GTSAC	7 cut(s)	595, 656, 727, 1243, 1853, 1927, 2422
TspDTI	ATGAA	9 cut(s)	692, 1115, 1184, 1230, 1475, 1656, 1809, 1917, 2100
TspGWI	ACGGA	2 cut(s)	485, 789
TspRI	CASTG	5 cut(s)	214, 620, 1252, 1873, 1977
Vha464I	CTTAAG	1 cut(s)	1417
VpaK11BI	GGWCC	5 cut(s)	257, 891, 1267, 1371, 2052
XagI	CCTNNNNNAGG	2 cut(s)	338, 2362
XapI	RAATTY	6 cut(s)	171, 230, 908, 2000, 2169, 2515
XbaI	TCTAGA	1 cut(s)	2111
XceI	RCATGY	3 cut(s)	493, 829, 1537
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1813
XhoI	CTCGAG	1 cut(s)	2497
XmiI	GTMKAC	1 cut(s)	1549
XmnI	GAANNNNTTC	2 cut(s)	696, 1908
XspI	CTAG	9 cut(s)	41, 111, 444, 1538, 1938, 2066, 2112, 2237, 2418

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.