Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0006469

Physical Location & Seq

Genomic Coordinates Forward (+)

14026663 .. 14033419

Gene Structure 6757 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1178200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2625 bp

ATGGAGAGCAATAGCAGCACCAGAGGAGTCTTTAGCGTCTCAATGTGCGGCCAAGAGCAGATTGAGCTCTCCAGAGACGGCAGCCACTACAGTCTCTCAACTGGGATTTTGCCTTCCCTCGGTGCAAGGTCGAGCAACCGACGCGTGAAACTCGGCAGGTTCATTGTCTCGCCGTACGACCGCCGCTACAGGATATGGGAGACATTTCTTGTTGTTCTGGTCATCTATACTGCTTGGGTGTCGCCGTTCGAGTTTGGTTTCCTTAAAGGACCAGGGGGACCGCTCTCCATTACTGATAATGTTGTCAATGGTTTCTTTGCTATTGATATCATTCTCACATTCTTTGTGGCTTACCTGGACAAGACCACATATTTACTTGTTGACGATCGAAAAAAGATTGGTTGGAAGTATGCAAGATCCTGGTTGATTTTCGATGTCATATCCACAATCCCATCTGAACTTGCTACGAGGATCTTTCCTAAATCTCTGCAGTCTTATGGCTTTTTCAACATGCTTCGTCTTTGGCGTCTACGAAGAGTTAGTGCCTTATTTTCCAGATTGGAGAAAGATAGGAATTATAATTACTTTTGGGTTCGCTGTGCAAAACTTATTTGTGTCACTCTTTTTGCCATCCATTGTGCTGGATGCTTCTATTACCTTATAGCTGCACGCAATCATGACCCTGCGAAAACATGGATGGGAGCTGAAATCCTAGGACAAAGCCTGTGGATTCGGTATGTGACTGCAGTTTATTGGTCAATCACAACGCTAACAACAGTTGGATATGGAGACCTGCATCCAGTCAATGCACGTGAGATGATCTTTGACATATTCTACATGTTCTTCAACCTGGGATTGACGTCATACTTAATTGGAAATATGACCAACTTGGTTGTCCACGGGACCAGTCGAACTAGAAAATTTAGGGATACCATACAAGCTGCCTCCAGTTTTGCGCAGAGGAATCAACTGCCTATACGCCTGCAGGATCAGATGCTTGCACACTTGTGTCTGAAGTTCAGAACAGATTCTGAAGGACTGCAGCAACAAGAGACCCTTGATTCCCTTCCTAAAGCCATCCGCTCGAGTATTTCACATTATCTGTTTTACTCTCTTGTAGATAAGGTGTACTTGTTTCATGGGGTTTCAAATGACTTGCTTTTTCAGTTGGTCTCGGAGATGAAAGCCGAGTATTTTCCTCCCAAAGAAGATGTAATCTTGCAGAACGAGGCACCCACAGATTTCTACCTACTTGTCACTGGTGCGGCGGATTTAGTGGTTCTCAAACATGGAGTTGAACAGGTCATTGGTGAGGCAAAATCTGGTGATCTTATTGGTGAGATCGGGGTACTTTGTTACAGACCACAGCTCTTTACAGTTAGAACCAAAAGATTGAGTCAGCTTCTACGGCTGAATCGTACTACATTCTTAAATATAGTTCAAGCTAATGTTGGAGATGGGACTGTGATCATGAATAATCTCCTGCAGCATTTGAAAGACCTCAAGGACCCAATTATGGAAGGAGTTCTGTCGGAGACGGAGAACATGCTAGCTCGGGGTAGAATGGACCTGCCTCTCAGTCTATGCTTTGCAGCAATCAGAGGAGACGACTTGTTGTTAAATCAGTTGCTGAAACGAGGTCTGGATCCAAATGAATCGGATAATAACGGGAGGTCTGCTCTGCATATAGCATCATCGAAAGGAAGCGAAAACTGCGTTCTTCTTCTGCTAGACTACGGGGCGGATCCTAAGAGTAGAGACTCAGATGGGAATGTACCACTTTGGGAGGCAATACTGAATGGTCATGAGCAAATTGCCAAGCTGCTGTTAGACAATGGTGCAAACTTGAACTCAGGGGATGTTGGTCAATTTGCTTGCACTGCTGCCGAGCAAAACAGGTTGGACTTGCTCAAGGAAATTGCCCGACATGGCGGGGATGTCACGCGTCCGAAAAGCAACGGAACCACAGCTCTGCATGTTGCAGTATCTGAAGACAATGTAGACATTGTCAAATACCTCTTGGACCAAGGCGCTGACATTGATAAACCCGACCTCCATGGCTGGACCCCGAGAGCCCTAGCGGAACAGCAAGGACACGAAGACATAAAGACCCTTTTCCACTCTAGTAAAGAACCCAAAGTCGCCATTACCATTCCCGAACACAAGAGTGGAATCCGGTTCCTTGGGAGGTTTACGAGCGAGCCAACTATCCACCCTCCACCCACAGACAGCTCGTTTTCAGGAGCGGAGGGAGGATCATGCGGCAGAAATCGCCCGAGGCGTAGGACTAATAACTTCCACAACTCTCTGTTTGGCATGATGTCAGCTGCCCACACCGGGGAGAAAGACTTGTTTCTCTCCGTTGCGGAGACGAAAAGTCCTAAGAGCTACGTAAGCAACCCTGCTGCTAGGGTGACAATTAGTTGCCCTGAAAAGGGTGAAGTGAAAGGCAAACTTGTGCTGCTCCCACTGAGTTATGAGGAGTTGCTTGAGATGGGGGCAAAGAAATTCGGACTCTCACCGGCGAAAATCGTGAGCAAAGATGGAGCCGAAATCGACGATATTGATGTAATTAGAGATGGTGATCATCTTGTTTTTGTAAGTGCTGGTGAATCACAACAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008324 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010035 GO:0010053 GO:0010054 GO:0010107 GO:0010119 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016049 GO:0021700 GO:0022622 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0034220 GO:0040007 GO:0042221 GO:0042391 GO:0042802 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071804 GO:0071805 GO:0071944 GO:0080147 GO:0090333 GO:0090558 GO:0090627 GO:0098655 GO:0098660 GO:0098662 GO:0099094 GO:0099402 GO:1901700 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

875

Amino Acids

97.73

Weight (kDa)

7.31

Isoelectric Point (pI)

38.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	63 - 305	1e-43	Ion transport protein
Ion_trans_2	PF07885	209 - 298	3.6e-13	Ion channel
cNMP_binding	PF00027	398 - 483	9.1e-14	Cyclic nucleotide-binding domain
Ank_2	PF12796	528 - 619	2.4e-18	Ankyrin repeats (3 copies)
Ank_4	PF13637	528 - 577	4.7e-08	Ankyrin repeats (many copies)
Ank_5	PF13857	543 - 594	6.5e-08	Ankyrin repeats (many copies)
Ank	PF00023	556 - 587	7e-06	Ankyrin repeat
Ank_2	PF12796	627 - 709	8.7e-13	Ankyrin repeats (3 copies)
Ank_4	PF13637	627 - 674	1.8e-06	Ankyrin repeats (many copies)
Ank_5	PF13857	642 - 694	7.7e-07	Ankyrin repeats (many copies)
Ank	PF00023	653 - 684	1.5e-08	Ankyrin repeat
KHA	PF11834	804 - 866	2.1e-24	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006469)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26650 AT2G26650
fragaria_vesca	FvH4_1g03960 FvH4_1g03960 FvH4_1g03960 FvH4_1g03960 FvH4_1g03960 FvH4_3g32370
malus_domestica	MD02G1037900.v1.1 MD15G1178200.v1.1
prunus_persica	Prupe.7G237400_v2.0.a1 Prupe.7G237400_v2.0.a1 Prupe.7G237400_v2.0.a1
pyrus_communis	pycom15g15990
rosa_chinensis	RchiOBHm_Chr2g0089421
rosa_laevigata	RLG00000016026
rosa_multiflora	Rmu_sc0000197.1_g000013
rosa_roxburghii	Rroxscaffold_2G00151950
rosa_rugosa	Rorug02G0000500 Rorug02G0000600 Rorug02G0000700
rosa_samantha	Rh2AG045800 Rh2BG044300 Rh2CG046200 Rh2DG045700
rosa_wichuraiana	Rw2G003680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	579
AatII	GACGTC	1 cut(s)	863
Acc16I	TGCGCA	1 cut(s)	957
Acc36I	ACCTGC	3 cut(s)	147, 801, 1578
AccB1I	GGYRCC	1 cut(s)	1231
AccBSI	CCGCTC	3 cut(s)	283, 1083, 2246
AccI	GTMKAC	2 cut(s)	529, 2001
AccII	CGCG	2 cut(s)	144, 1945
AclWI	GGATC	8 cut(s)	411, 479, 996, 1640, 1653, 1739, 1752, 2263
AcoI	YGGCCR	1 cut(s)	49
AcsI	RAATTY	2 cut(s)	920, 2507
AcuI	CTGAAG	3 cut(s)	1034, 1053, 2010
AcvI	CACGTG	1 cut(s)	812
AcyI	GRCGYC	2 cut(s)	526, 860
AfaI	GTAC	5 cut(s)	176, 1130, 1350, 1420, 1776
AfiI	CCNNNNNNNGG	6 cut(s)	119, 1516, 2337, 2338, 2434, 2435
AflIII	ACRYGT	3 cut(s)	142, 837, 1943
AgsI	TTSAA	7 cut(s)	508, 847, 1149, 1298, 1442, 1495, 1849
AhdI	GACNNNNNGTC	1 cut(s)	2376
AjnI	CCWGG	4 cut(s)	271, 354, 419, 849
AjuI	GAANNNNNNNTTGG	2 cut(s)	1290, 1322
AleI	CACNNNNGTG	2 cut(s)	1006, 2166
Alw21I	GWGCWC	1 cut(s)	69
AlwI	GGATC	8 cut(s)	411, 479, 996, 1640, 1653, 1739, 1752, 2263
AlwNI	CAGNNNCTG	3 cut(s)	1031, 1630, 1988
Ama87I	CYCGRG	4 cut(s)	1084, 1554, 2068, 2275
AoxI	GGCC	1 cut(s)	49
ApoI	RAATTY	2 cut(s)	920, 2507
Asp700I	GAANNNNTTC	2 cut(s)	1027, 1524
AspA2I	CCTAGG	1 cut(s)	712
AspLEI	GCGC	2 cut(s)	958, 2033
AspS9I	GGNCC	7 cut(s)	269, 278, 903, 1507, 1567, 2023, 2064
AsuC2I	CCSGG	1 cut(s)	2338
AsuHPI	GGTGA	8 cut(s)	1322, 1337, 1349, 2425, 2450, 2511, 2594, 2621
AsuNHI	GCTAGC	1 cut(s)	1549
AvaI	CYCGRG	4 cut(s)	1084, 1554, 2068, 2275
AvaII	GGWCC	7 cut(s)	269, 278, 903, 1507, 1567, 2023, 2064
AvrII	CCTAGG	1 cut(s)	712
BamHI	GGATCC	2 cut(s)	1645, 1744
BanI	GGYRCC	1 cut(s)	1231
BanII	GRGCYC	2 cut(s)	69, 2077
BbrPI	CACGTG	1 cut(s)	812
BbsI	GAAGAC	2 cut(s)	1998, 2106
Bbv12I	GWGCWC	1 cut(s)	69
BccI	CCATC	9 cut(s)	460, 638, 691, 1085, 1451, 1760, 2488, 2537, 2573
BceAI	ACGGC	4 cut(s)	94, 157, 229, 1424
BciT130I	CCWGG	4 cut(s)	273, 356, 421, 851
BciVI	GTATCC	1 cut(s)	922
BclI	TGATCA	2 cut(s)	1467, 2584
BcnI	CCSGG	1 cut(s)	2338
BfaI	CTAG	7 cut(s)	713, 915, 1550, 1730, 2078, 2124, 2409
BfmI	CTRYAG	7 cut(s)	88, 187, 488, 744, 983, 1040, 1484
BfoI	RGCGCY	1 cut(s)	2034
BfuAI	ACCTGC	3 cut(s)	147, 801, 1578
BfuI	GTATCC	1 cut(s)	922
BlnI	CCTAGG	1 cut(s)	712
Bme1390I	CCNGG	5 cut(s)	273, 356, 421, 851, 2338
Bme18I	GGWCC	7 cut(s)	269, 278, 903, 1507, 1567, 2023, 2064
BmeRI	GACNNNNNGTC	1 cut(s)	2376
BmeT110I	CYCGRG	4 cut(s)	1084, 1554, 2068, 2275
BmgT120I	GGNCC	7 cut(s)	269, 278, 903, 1507, 1567, 2023, 2064
BmrFI	CCNGG	5 cut(s)	273, 356, 421, 851, 2338
BmrI	ACTGGG	1 cut(s)	111
BmsI	GCATC	4 cut(s)	635, 805, 984, 1700
BmtI	GCTAGC	1 cut(s)	1553
BmuI	ACTGGG	1 cut(s)	111
BpiI	GAAGAC	2 cut(s)	1998, 2106
BplI	GAGNNNNNCTC	2 cut(s)	1663, 1695
BpmI	CTGGAG	2 cut(s)	55, 931
BpuEI	CTTGAG	3 cut(s)	1487, 1895, 2510
BpuMI	CCSGG	1 cut(s)	2338
BsaAI	YACGTR	2 cut(s)	812, 2392
BsaBI	GATNNNNATC	1 cut(s)	2583
BsaHI	GRCGYC	2 cut(s)	526, 860
BsaI	GGTCTC	3 cut(s)	783, 1046, 1177
BsaWI	WCCGGW	1 cut(s)	2175
BsaXI	ACNNNNNCTCC	2 cut(s)	2037, 2067
Bsc4I	CCNNNNNNNGG	6 cut(s)	119, 1516, 2337, 2338, 2434, 2435
Bse118I	RCCGGY	1 cut(s)	2521
Bse1I	ACTGG	5 cut(s)	106, 800, 906, 948, 1264
Bse8I	GATNNNNATC	1 cut(s)	2583
BseBI	CCWGG	4 cut(s)	273, 356, 421, 851
BseGI	GGATG	7 cut(s)	630, 650, 702, 796, 1077, 1864, 1942
BseJI	GATNNNNATC	1 cut(s)	2583
BseLI	CCNNNNNNNGG	6 cut(s)	119, 1516, 2337, 2338, 2434, 2435
BseMII	CTCAG	4 cut(s)	1591, 1776, 1866, 2462
BseNI	ACTGG	5 cut(s)	106, 800, 906, 948, 1264
BseRI	GAGGAG	3 cut(s)	39, 1617, 2495
BsgI	GTGCAG	1 cut(s)	651
Bsh1236I	CGCG	2 cut(s)	144, 1945
Bsh1285I	CGRYCG	2 cut(s)	181, 388
BshFI	GGCC	1 cut(s)	51
BshNI	GGYRCC	1 cut(s)	1231
BsiEI	CGRYCG	2 cut(s)	181, 388
BsiHKAI	GWGCWC	1 cut(s)	69
BsiHKCI	CYCGRG	4 cut(s)	1084, 1554, 2068, 2275
BsiSI	CCGG	3 cut(s)	2176, 2337, 2522
BsiWI	CGTACG	1 cut(s)	174
BslFI	GGGAC	3 cut(s)	291, 916, 1474
BslI	CCNNNNNNNGG	6 cut(s)	119, 1516, 2337, 2338, 2434, 2435
BsmBI	CGTCTC	5 cut(s)	43, 69, 1529, 1599, 2363
BsmFI	GGGAC	3 cut(s)	291, 916, 1474
BsnI	GGCC	1 cut(s)	51
Bso31I	GGTCTC	3 cut(s)	783, 1046, 1177
BsoBI	CYCGRG	4 cut(s)	1084, 1554, 2068, 2275
Bsp1286I	GDGCHC	2 cut(s)	69, 2077
Bsp19I	CCATGG	1 cut(s)	2056
BspANI	GGCC	1 cut(s)	51
BspCNI	CTCAG	4 cut(s)	1590, 1775, 1865, 2463
BspFNI	CGCG	2 cut(s)	144, 1945
BspHI	TCATGA	3 cut(s)	676, 1470, 1804
BspMAI	CTGCAG	5 cut(s)	492, 748, 987, 1044, 1488
BspMI	ACCTGC	3 cut(s)	147, 801, 1578
BspOI	GCTAGC	1 cut(s)	1553
BspPI	GGATC	8 cut(s)	411, 479, 996, 1640, 1653, 1739, 1752, 2263
BspT107I	GGYRCC	1 cut(s)	1231
BspTNI	GGTCTC	3 cut(s)	783, 1046, 1177
BsrBI	CCGCTC	3 cut(s)	283, 1083, 2246
BsrFI	RCCGGY	1 cut(s)	2521
BsrI	ACTGG	5 cut(s)	106, 800, 906, 948, 1264
BssAI	RCCGGY	1 cut(s)	2521
BssNI	GRCGYC	2 cut(s)	526, 860
BssT1I	CCWWGG	4 cut(s)	712, 2026, 2056, 2182
Bst2UI	CCWGG	4 cut(s)	273, 356, 421, 851
Bst4CI	ACNGT	4 cut(s)	92, 778, 1378, 1465
Bst6I	CTCTTC	1 cut(s)	529
BstACI	GRCGYC	2 cut(s)	526, 860
BstBAI	YACGTR	2 cut(s)	812, 2392
BstC8I	GCNNGC	6 cut(s)	670, 983, 999, 1551, 1876, 2201
BstDEI	CTNAG	6 cut(s)	1577, 1749, 1762, 1852, 2382, 2471
BstDSI	CCRYGG	2 cut(s)	898, 2056
BstF5I	GGATG	7 cut(s)	630, 650, 702, 796, 1077, 1864, 1942
BstFNI	CGCG	2 cut(s)	144, 1945
BstH2I	RGCGCY	1 cut(s)	2034
BstHHI	GCGC	2 cut(s)	958, 2033
BstMCI	CGRYCG	2 cut(s)	181, 388
BstMWI	GCNNNNNNNGC	8 cut(s)	15, 64, 141, 1408, 1713, 1880, 2271, 2394
BstNI	CCWGG	4 cut(s)	273, 356, 421, 851
BstNSI	RCATGY	4 cut(s)	514, 841, 1549, 1979
BstSCI	CCNGG	5 cut(s)	271, 354, 419, 849, 2336
BstSFI	CTRYAG	7 cut(s)	88, 187, 488, 744, 983, 1040, 1484
BstSNI	TACGTA	1 cut(s)	2392
BstUI	CGCG	2 cut(s)	144, 1945
BstV2I	GAAGAC	2 cut(s)	1998, 2106
BstX2I	RGATCY	4 cut(s)	416, 471, 1645, 1744
BstXI	CCANNNNNNTGG	1 cut(s)	641
BstYI	RGATCY	4 cut(s)	416, 471, 1645, 1744
BsuI	GTATCC	1 cut(s)	922
BsuRI	GGCC	1 cut(s)	51
BtgI	CCRYGG	2 cut(s)	898, 2056
BtsCI	GGATG	7 cut(s)	630, 650, 702, 796, 1077, 1864, 1942
BtsI	GCAGTG	1 cut(s)	1878
BtsIMutI	CAGTG	3 cut(s)	1257, 1878, 2468
BveI	ACCTGC	3 cut(s)	147, 801, 1578
Cac8I	GCNNGC	6 cut(s)	670, 983, 999, 1551, 1876, 2201
CaiI	CAGNNNCTG	3 cut(s)	1031, 1630, 1988
CciI	TCATGA	3 cut(s)	676, 1470, 1804
CfoI	GCGC	2 cut(s)	958, 2033
Cfr10I	RCCGGY	1 cut(s)	2521
Cfr13I	GGNCC	7 cut(s)	269, 278, 903, 1507, 1567, 2023, 2064
CseI	GACGC	4 cut(s)	25, 150, 515, 1934
Csp6I	GTAC	5 cut(s)	175, 1129, 1349, 1419, 1775
CspCI	CAANNNNNGTGG	2 cut(s)	707, 742
CviQI	GTAC	5 cut(s)	175, 1129, 1349, 1419, 1775
DdeI	CTNAG	6 cut(s)	1577, 1749, 1762, 1852, 2382, 2471
DriI	GACNNNNNGTC	1 cut(s)	2376
EaeI	YGGCCR	1 cut(s)	49
Eam1104I	CTCTTC	1 cut(s)	529
Eam1105I	GACNNNNNGTC	1 cut(s)	2376
EarI	CTCTTC	1 cut(s)	529
EciI	GGCGGA	2 cut(s)	1283, 1757
Ecl136II	GAGCTC	1 cut(s)	67
Eco105I	TACGTA	1 cut(s)	2392
Eco130I	CCWWGG	4 cut(s)	712, 2026, 2056, 2182
Eco24I	GRGCYC	2 cut(s)	69, 2077
Eco31I	GGTCTC	3 cut(s)	783, 1046, 1177
Eco32I	GATATC	1 cut(s)	328
Eco47I	GGWCC	7 cut(s)	269, 278, 903, 1507, 1567, 2023, 2064
Eco53kI	GAGCTC	1 cut(s)	67
Eco57I	CTGAAG	3 cut(s)	1034, 1053, 2010
Eco72I	CACGTG	1 cut(s)	812
Eco88I	CYCGRG	4 cut(s)	1084, 1554, 2068, 2275
EcoICRI	GAGCTC	1 cut(s)	67
EcoO109I	RGGNCCY	1 cut(s)	1507
EcoRII	CCWGG	4 cut(s)	271, 354, 419, 849
EcoRV	GATATC	1 cut(s)	328
EcoT14I	CCWWGG	4 cut(s)	712, 2026, 2056, 2182
EcoT38I	GRGCYC	2 cut(s)	69, 2077
ErhI	CCWWGG	4 cut(s)	712, 2026, 2056, 2182
Esp3I	CGTCTC	5 cut(s)	43, 69, 1529, 1599, 2363
FalI	AAGNNNNNCTT	6 cut(s)	1041, 1073, 1115, 1147, 2439, 2471
FaqI	GGGAC	3 cut(s)	291, 916, 1474
FauI	CCCGC	1 cut(s)	1925
FbaI	TGATCA	2 cut(s)	1467, 2584
FblI	GTMKAC	2 cut(s)	529, 2001
FokI	GGATG	7 cut(s)	617, 657, 709, 783, 1064, 1871, 1949
FriOI	GRGCYC	2 cut(s)	69, 2077
FspBI	CTAG	7 cut(s)	713, 915, 1550, 1730, 2078, 2124, 2409
FspI	TGCGCA	1 cut(s)	957
GlaI	GCGC	2 cut(s)	957, 2032
GsuI	CTGGAG	2 cut(s)	55, 931
HaeII	RGCGCY	1 cut(s)	2034
HaeIII	GGCC	1 cut(s)	51
HapII	CCGG	3 cut(s)	2176, 2337, 2522
HgaI	GACGC	4 cut(s)	25, 150, 515, 1934
HhaI	GCGC	2 cut(s)	958, 2033
Hin1I	GRCGYC	2 cut(s)	526, 860
Hin6I	GCGC	2 cut(s)	956, 2031
HinP1I	GCGC	2 cut(s)	956, 2031
HincII	GTYRAC	1 cut(s)	382
HindII	GTYRAC	1 cut(s)	382
HpaII	CCGG	3 cut(s)	2176, 2337, 2522
HphI	GGTGA	8 cut(s)	1322, 1337, 1349, 2425, 2450, 2511, 2594, 2621
Hpy166II	GTNNAC	6 cut(s)	382, 530, 898, 1129, 2002, 2193
Hpy188III	TCNNGA	9 cut(s)	72, 555, 677, 1471, 1643, 1805, 2156, 2241, 2533
Hpy8I	GTNNAC	6 cut(s)	382, 530, 898, 1129, 2002, 2193
Hpy99I	CGWCG	2 cut(s)	144, 2561
HpyAV	CCTTC	4 cut(s)	123, 1028, 1076, 1514
HpyCH4III	ACNGT	4 cut(s)	92, 778, 1378, 1465
HpyCH4IV	ACGT	3 cut(s)	811, 860, 2391
HpyF10VI	GCNNNNNNNGC	8 cut(s)	15, 64, 141, 1408, 1713, 1880, 2271, 2394
HpyF3I	CTNAG	6 cut(s)	1577, 1749, 1762, 1852, 2382, 2471
HpySE526I	ACGT	3 cut(s)	811, 860, 2391
Hsp92I	GRCGYC	2 cut(s)	526, 860
HspAI	GCGC	2 cut(s)	956, 2031
Ksp22I	TGATCA	2 cut(s)	1467, 2584
LmnI	GCTCC	4 cut(s)	701, 2243, 2469, 2546
LweI	GCATC	4 cut(s)	635, 805, 984, 1700
MaeI	CTAG	7 cut(s)	713, 915, 1550, 1730, 2078, 2124, 2409
MaeII	ACGT	3 cut(s)	811, 860, 2391
MaeIII	GTNAC	6 cut(s)	616, 739, 1255, 1355, 1939, 2413
MbiI	CCGCTC	3 cut(s)	283, 1083, 2246
MboII	GAAGA	7 cut(s)	546, 835, 1220, 1712, 1715, 2003, 2111
MflI	RGATCY	4 cut(s)	416, 471, 1645, 1744
MhlI	GDGCHC	2 cut(s)	69, 2077
MluI	ACGCGT	2 cut(s)	142, 1943
MlyI	GAGTC	4 cut(s)	36, 1405, 1754, 2508
MmeI	TCCRAC	5 cut(s)	383, 760, 1432, 1512, 1881
MroXI	GAANNNNTTC	2 cut(s)	1027, 1524
MseI	TTAA	4 cut(s)	264, 869, 1430, 1619
MslI	CAYNNNNRTG	2 cut(s)	1006, 2166
MspA1I	CMGCKG	1 cut(s)	2327
MspI	CCGG	3 cut(s)	2176, 2337, 2522
MspR9I	CCNGG	5 cut(s)	273, 356, 421, 851, 2338
MvaI	CCWGG	4 cut(s)	273, 356, 421, 851
MvnI	CGCG	2 cut(s)	144, 1945
MwoI	GCNNNNNNNGC	8 cut(s)	15, 64, 141, 1408, 1713, 1880, 2271, 2394
NciI	CCSGG	1 cut(s)	2338
NcoI	CCATGG	1 cut(s)	2056
NheI	GCTAGC	1 cut(s)	1549
NmeAIII	GCCGAG	3 cut(s)	132, 1213, 1912
NmuCI	GTSAC	5 cut(s)	616, 739, 1255, 1939, 2413
NsbI	TGCGCA	1 cut(s)	957
NspI	RCATGY	4 cut(s)	514, 841, 1549, 1979
OliI	CACNNNNGTG	2 cut(s)	1006, 2166
PaeR7I	CTCGAG	1 cut(s)	1084
PagI	TCATGA	3 cut(s)	676, 1470, 1804
PciI	ACATGT	1 cut(s)	837
PcsI	WCGNNNNNNNCGW	3 cut(s)	523, 1414, 2278
PdmI	GAANNNNTTC	2 cut(s)	1027, 1524
PfeI	GAWTC	8 cut(s)	730, 964, 1028, 1061, 1414, 1655, 2172, 2612
Pfl23II	CGTACG	1 cut(s)	174
PflFI	GACNNNGTC	1 cut(s)	2006
Ple19I	CGATCG	1 cut(s)	388
PleI	GAGTC	4 cut(s)	35, 1404, 1754, 2508
PmaCI	CACGTG	1 cut(s)	812
PmlI	CACGTG	1 cut(s)	812
PpsI	GAGTC	4 cut(s)	35, 1404, 1754, 2508
Ppu21I	YACGTR	2 cut(s)	812, 2392
PpuMI	RGGWCCY	1 cut(s)	1507
PscI	ACATGT	1 cut(s)	837
PsiI	TTATAA	1 cut(s)	579
Psp124BI	GAGCTC	1 cut(s)	69
Psp5II	RGGWCCY	1 cut(s)	1507
Psp6I	CCWGG	4 cut(s)	271, 354, 419, 849
PspCI	CACGTG	1 cut(s)	812
PspGI	CCWGG	4 cut(s)	271, 354, 419, 849
PspLI	CGTACG	1 cut(s)	174
PspPI	GGNCC	7 cut(s)	269, 278, 903, 1507, 1567, 2023, 2064
PspPPI	RGGWCCY	1 cut(s)	1507
PspXI	VCTCGAGB	1 cut(s)	1084
PstI	CTGCAG	5 cut(s)	492, 748, 987, 1044, 1488
PstNI	CAGNNNCTG	3 cut(s)	1031, 1630, 1988
PsuI	RGATCY	4 cut(s)	416, 471, 1645, 1744
PsyI	GACNNNGTC	1 cut(s)	2006
PvuI	CGATCG	1 cut(s)	388
PvuII	CAGCTG	1 cut(s)	2327
RsaI	GTAC	5 cut(s)	176, 1130, 1350, 1420, 1776
RsaNI	GTAC	5 cut(s)	175, 1129, 1349, 1419, 1775
RseI	CAYNNNNRTG	2 cut(s)	1006, 2166
SacI	GAGCTC	1 cut(s)	69
SaqAI	TTAA	4 cut(s)	264, 869, 1430, 1619
Sau96I	GGNCC	7 cut(s)	269, 278, 903, 1507, 1567, 2023, 2064
SbfI	CCTGCAGG	1 cut(s)	987
SchI	GAGTC	4 cut(s)	36, 1405, 1754, 2508
ScrFI	CCNGG	5 cut(s)	273, 356, 421, 851, 2338
SdaI	CCTGCAGG	1 cut(s)	987
SduI	GDGCHC	2 cut(s)	69, 2077
SfaNI	GCATC	4 cut(s)	635, 805, 984, 1700
SfcI	CTRYAG	7 cut(s)	88, 187, 488, 744, 983, 1040, 1484
Sfr274I	CTCGAG	1 cut(s)	1084
SgrAI	CRCCGGYG	1 cut(s)	2521
SinI	GGWCC	7 cut(s)	269, 278, 903, 1507, 1567, 2023, 2064
SlaI	CTCGAG	1 cut(s)	1084
SmiMI	CAYNNNNRTG	2 cut(s)	1006, 2166
SmlI	CTYRAG	4 cut(s)	1084, 1502, 1910, 2489
SmoI	CTYRAG	4 cut(s)	1084, 1502, 1910, 2489
SnaBI	TACGTA	1 cut(s)	2392
Sse8387I	CCTGCAGG	1 cut(s)	987
SspMI	CTAG	7 cut(s)	713, 915, 1550, 1730, 2078, 2124, 2409
SstI	GAGCTC	1 cut(s)	69
StyD4I	CCNGG	5 cut(s)	271, 354, 419, 849, 2336
StyI	CCWWGG	4 cut(s)	712, 2026, 2056, 2182
TaaI	ACNGT	4 cut(s)	92, 778, 1378, 1465
TaiI	ACGT	3 cut(s)	814, 863, 2394
TaqI	TCGA	8 cut(s)	131, 249, 388, 432, 910, 1085, 1697, 2556
TatI	WGTACW	1 cut(s)	1128
TauI	GCSGC	4 cut(s)	51, 186, 1268, 2265
TfiI	GAWTC	8 cut(s)	730, 964, 1028, 1061, 1414, 1655, 2172, 2612
Tru1I	TTAA	4 cut(s)	264, 869, 1430, 1619
Tru9I	TTAA	4 cut(s)	264, 869, 1430, 1619
TscAI	CASTG	3 cut(s)	1264, 1885, 2475
TseFI	GTSAC	5 cut(s)	616, 739, 1255, 1939, 2413
Tsp45I	GTSAC	5 cut(s)	616, 739, 1255, 1939, 2413
TspDTI	ATGAA	5 cut(s)	151, 1127, 1196, 1487, 1668
TspGWI	ACGGA	3 cut(s)	1553, 1974, 2350
TspRI	CASTG	3 cut(s)	1264, 1885, 2475
Tth111I	GACNNNGTC	1 cut(s)	2006
VpaK11BI	GGWCC	7 cut(s)	269, 278, 903, 1507, 1567, 2023, 2064
XapI	RAATTY	2 cut(s)	920, 2507
XceI	RCATGY	4 cut(s)	514, 841, 1549, 1979
XhoI	CTCGAG	1 cut(s)	1084
XmaJI	CCTAGG	1 cut(s)	712
XmiI	GTMKAC	2 cut(s)	529, 2001
XmnI	GAANNNNTTC	2 cut(s)	1027, 1524
XspI	CTAG	7 cut(s)	713, 915, 1550, 1730, 2078, 2124, 2409
ZraI	GACGTC	1 cut(s)	861

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.