FvH4_1g04221

Transmembrane protein 19-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0015870
Physical Location & Seq
Forward (+)
2236863 .. 2238652
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04221.t1

Sequence Viewer

Length: 864 bp
ATGGACTCGCATCTAATCCAGCCCTTGATCGCCTTCCTAATCTCGGCCTTCGTGGCCGCCCGTGCCTACCGCCGGAAATCACTCGACTTTTCCGGCGCAGTCGCCGGCCTCGCCGTCATGACTCTCCACATTGCAGTCGGATACAGATACGGAGCCGTGTTGCTCGTCTTCTTCTTCACGTCGTCGAAGCTCACCAAGGTTGGCGAAGAGAAGAAGCGCCGGGTTGACCCGGAATTCAAAGAAGGCGGTCAACGCAATTGGATACAGGTGCTATCGAATGCAGGGATTTCATCAGTGTTGGTTGTGGTGCTGTGGAGGCTGACGGGGTTGGAAGAGAGATGCTTGGACTCGAAGGAGTCGGTTTTGGTTACGGCACTGATGGGAGGGGTTATAGGACACTTCTCTTGCTGCAATGGAGACACTTGGTCTTCTGAGCTTGGAATTCTTAGTGATGATCAGCCTCGTTTGATTACTACTTTCAAGCCTGTGCGGAAAGGTACCAATGGCGGAGTTACTAAAGCAGGATTACTGGCAGCTGCTGCAGCAGGGTCTGTTATCGGATTGGCGTTTGTGCTTGTTGGCTTTCTGACTACAAAATGTACATATGATGTAGCACTGAAGCAGCTGTTTGTGATACCTCTTTCTACCCTTGCTGGACTTTGTGGTAGTGTCATTGATTCTCTTCTGGGAGCAACACTGCAATTCAGTGGATTCTGTACTGTTCGTAACAAGGTTGTTGGCAAACCAGGACCTACTGTCAAGAAGATATCTGGCCTCAATTTTCTTGACAACAATGCCGTAAATGTTGTGTCTATCCTGATAACAACAATCCTCACTTCCATTGCCTGCATTTACGTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF92

Protein Analysis

288

Amino Acids

30.58

Weight (kDa)

9.38

Isoelectric Point (pI)

26.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF92 PF01940 14 - 275 1.2e-78 Integral membrane protein DUF92
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015870)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19930 AT5G19930
fragaria_vesca FvH4_1g04221
malus_domestica MD02G1061500.v1.1
prunus_persica Prupe.7G235100_v2.0.a1
pyrus_communis pycom15g16280
rosa_chinensis RchiOBHm_Chr2g0089771
rosa_laevigata RLG00000016054
rosa_roxburghii Rroxscaffold_2G00151630
rosa_rugosa Rorug02G0003100
rosa_samantha Rh2AG048300 Rh2BG047100 Rh2CG049200 Rh2DG048600
rosa_wichuraiana Rw2G004210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 497
AccB1I GGYRCC 1 cut(s) 497
AciI CCGC 5 cut(s) 57, 70, 246, 490, 507
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 2 cut(s) 233, 441
AcuI CTGAAG 1 cut(s) 638
AfaI GTAC 3 cut(s) 499, 601, 718
AfiI CCNNNNNNNGG 2 cut(s) 43, 72
AgsI TTSAA 2 cut(s) 238, 481
AhdI GACNNNNNGTC 2 cut(s) 424, 755
AjiI CACGTC 1 cut(s) 180
AjnI CCWGG 1 cut(s) 745
AluBI AGCT 4 cut(s) 190, 436, 536, 625
AluI AGCT 4 cut(s) 190, 436, 536, 625
Alw26I GTCTC 1 cut(s) 411
AlwNI CAGNNNCTG 2 cut(s) 539, 551
AoxI GGCC 4 cut(s) 45, 54, 106, 772
ApeKI GCWGC 6 cut(s) 408, 533, 536, 539, 542, 622
ApoI RAATTY 2 cut(s) 233, 441
Asp718I GGTACC 1 cut(s) 497
AspLEI GCGC 2 cut(s) 98, 219
AspS9I GGNCC 1 cut(s) 749
AsuC2I CCSGG 2 cut(s) 221, 230
AsuHPI GGTGA 1 cut(s) 184
AvaII GGWCC 1 cut(s) 749
BanI GGYRCC 1 cut(s) 497
BbsI GAAGAC 2 cut(s) 160, 420
BbvI GCAGC 6 cut(s) 395, 523, 526, 545, 554, 634
BccI CCATC 1 cut(s) 373
BceAI ACGGC 4 cut(s) 98, 140, 387, 782
BciT130I CCWGG 1 cut(s) 747
BciVI GTATCC 2 cut(s) 134, 255
BclI TGATCA 1 cut(s) 454
BcnI CCSGG 2 cut(s) 221, 230
BcoDI GTCTC 1 cut(s) 411
BfmI CTRYAG 1 cut(s) 540
BfoI RGCGCY 1 cut(s) 220
BfuI GTATCC 2 cut(s) 134, 255
BglI GCCNNNNNGGC 1 cut(s) 53
BisI GCNGC 7 cut(s) 57, 409, 534, 537, 540, 543, 623
BlsI GCNGC 7 cut(s) 58, 410, 535, 538, 541, 544, 624
Bme1390I CCNGG 3 cut(s) 221, 230, 747
Bme18I GGWCC 1 cut(s) 749
BmeRI GACNNNNNGTC 2 cut(s) 424, 755
BmgBI CACGTC 1 cut(s) 180
BmgT120I GGNCC 1 cut(s) 749
BmiI GGNNCC 2 cut(s) 154, 499
BmrFI CCNGG 3 cut(s) 221, 230, 747
BmsI GCATC 2 cut(s) 19, 329
BpiI GAAGAC 2 cut(s) 160, 420
BpuMI CCSGG 2 cut(s) 221, 230
BsaJI CCNNGG 1 cut(s) 195
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 72
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 1 cut(s) 534
Bse3DI GCAATG 3 cut(s) 129, 418, 840
BseBI CCWGG 1 cut(s) 747
BseDI CCNNGG 1 cut(s) 195
BseLI CCNNNNNNNGG 2 cut(s) 43, 72
BseMI GCAATG 3 cut(s) 129, 418, 840
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 1 cut(s) 534
BseXI GCAGC 6 cut(s) 395, 523, 526, 545, 554, 634
BshFI GGCC 4 cut(s) 47, 56, 108, 774
BshNI GGYRCC 1 cut(s) 497
BsiSI CCGG 5 cut(s) 73, 93, 105, 220, 230
BslI CCNNNNNNNGG 2 cut(s) 43, 72
BsmAI GTCTC 1 cut(s) 411
BsmI GAATGC 1 cut(s) 283
BsnI GGCC 4 cut(s) 47, 56, 108, 774
Bsp1407I TGTACA 1 cut(s) 599
Bsp143I GATC 2 cut(s) 27, 454
BspACI CCGC 5 cut(s) 57, 70, 246, 490, 507
BspANI GGCC 4 cut(s) 47, 56, 108, 774
BspCNI CTCAG 1 cut(s) 424
BspHI TCATGA 1 cut(s) 117
BspLI GGNNCC 2 cut(s) 154, 499
BspMAI CTGCAG 1 cut(s) 544
BspT107I GGYRCC 1 cut(s) 497
BsrDI GCAATG 3 cut(s) 129, 418, 840
BsrFI RCCGGY 1 cut(s) 104
BsrGI TGTACA 1 cut(s) 599
BsrI ACTGG 1 cut(s) 534
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 2 cut(s) 27, 454
BssT1I CCWWGG 1 cut(s) 195
Bst2UI CCWGG 1 cut(s) 747
Bst4CI ACNGT 2 cut(s) 721, 757
Bst6I CTCTTC 3 cut(s) 201, 327, 687
BstAPI GCANNNNNTGC 1 cut(s) 539
BstAUI TGTACA 1 cut(s) 599
BstC8I GCNNGC 2 cut(s) 106, 847
BstDEI CTNAG 2 cut(s) 432, 446
BstH2I RGCGCY 1 cut(s) 220
BstHHI GCGC 2 cut(s) 98, 219
BstKTI GATC 2 cut(s) 30, 457
BstMAI GTCTC 1 cut(s) 411
BstMBI GATC 2 cut(s) 27, 454
BstMWI GCNNNNNNNGC 7 cut(s) 53, 62, 110, 252, 316, 539, 542
BstNI CCWGG 1 cut(s) 747
BstSCI CCNGG 3 cut(s) 219, 228, 745
BstSFI CTRYAG 1 cut(s) 540
BstV1I GCAGC 6 cut(s) 395, 523, 526, 545, 554, 634
BstV2I GAAGAC 2 cut(s) 160, 420
BsuI GTATCC 2 cut(s) 134, 255
BsuRI GGCC 4 cut(s) 47, 56, 108, 774
BtrI CACGTC 1 cut(s) 180
BtsI GCAGTG 1 cut(s) 695
BtsIMutI CAGTG 5 cut(s) 300, 374, 614, 695, 712
Cac8I GCNNGC 2 cut(s) 106, 847
CaiI CAGNNNCTG 2 cut(s) 539, 551
CciI TCATGA 1 cut(s) 117
CfoI GCGC 2 cut(s) 98, 219
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 1 cut(s) 749
Csp6I GTAC 3 cut(s) 498, 600, 717
CviAII CATG 1 cut(s) 118
CviQI GTAC 3 cut(s) 498, 600, 717
DdeI CTNAG 2 cut(s) 432, 446
DpnI GATC 2 cut(s) 29, 456
DpnII GATC 2 cut(s) 27, 454
DriI GACNNNNNGTC 2 cut(s) 424, 755
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 3 cut(s) 201, 327, 687
Eam1105I GACNNNNNGTC 2 cut(s) 424, 755
EarI CTCTTC 3 cut(s) 201, 327, 687
EciI GGCGGA 1 cut(s) 522
Eco130I CCWWGG 1 cut(s) 195
Eco32I GATATC 1 cut(s) 768
Eco47I GGWCC 1 cut(s) 749
Eco57I CTGAAG 1 cut(s) 638
EcoO109I RGGNCCY 1 cut(s) 749
EcoRI GAATTC 2 cut(s) 233, 441
EcoRII CCWGG 1 cut(s) 745
EcoRV GATATC 1 cut(s) 768
EcoT14I CCWWGG 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 1 cut(s) 121
FaiI YATR 4 cut(s) 119, 392, 604, 606
FatI CATG 1 cut(s) 117
FauNDI CATATG 1 cut(s) 604
FbaI TGATCA 1 cut(s) 454
Fnu4HI GCNGC 7 cut(s) 57, 409, 534, 537, 540, 543, 623
Fsp4HI GCNGC 7 cut(s) 57, 409, 534, 537, 540, 543, 623
GlaI GCGC 2 cut(s) 97, 218
GluI GCNGC 7 cut(s) 57, 409, 534, 537, 540, 543, 623
HaeII RGCGCY 1 cut(s) 220
HaeIII GGCC 4 cut(s) 47, 56, 108, 774
HapII CCGG 5 cut(s) 73, 93, 105, 220, 230
HhaI GCGC 2 cut(s) 98, 219
Hin1II CATG 1 cut(s) 121
Hin6I GCGC 2 cut(s) 96, 217
HinP1I GCGC 2 cut(s) 96, 217
HincII GTYRAC 2 cut(s) 226, 251
HindII GTYRAC 2 cut(s) 226, 251
HinfI GANTC 6 cut(s) 5, 121, 347, 356, 677, 711
HpaII CCGG 5 cut(s) 73, 93, 105, 220, 230
HphI GGTGA 1 cut(s) 184
Hpy166II GTNNAC 2 cut(s) 226, 251
Hpy188I TCNGA 4 cut(s) 140, 433, 560, 588
Hpy188III TCNNGA 4 cut(s) 118, 760, 785, 817
Hpy8I GTNNAC 2 cut(s) 226, 251
Hpy99I CGWCG 2 cut(s) 184, 187
HpyAV CCTTC 4 cut(s) 43, 58, 236, 346
HpyCH4III ACNGT 2 cut(s) 721, 757
HpyCH4IV ACGT 2 cut(s) 179, 855
HpyCH4V TGCA 6 cut(s) 134, 281, 411, 542, 700, 849
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 62, 110, 252, 316, 539, 542
HpyF3I CTNAG 2 cut(s) 432, 446
HpySE526I ACGT 2 cut(s) 179, 855
Hsp92II CATG 1 cut(s) 121
HspAI GCGC 2 cut(s) 96, 217
KpnI GGTACC 1 cut(s) 501
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 454
Kzo9I GATC 2 cut(s) 27, 454
LmnI GCTCC 2 cut(s) 152, 689
Lsp1109I GCAGC 6 cut(s) 395, 523, 526, 545, 554, 634
LweI GCATC 2 cut(s) 19, 329
MaeII ACGT 2 cut(s) 179, 855
MaeIII GTNAC 3 cut(s) 367, 511, 725
MalI GATC 2 cut(s) 29, 456
MboI GATC 2 cut(s) 27, 454
MboII GAAGA 9 cut(s) 160, 163, 166, 218, 223, 344, 420, 674, 775
MfeI CAATTG 1 cut(s) 256
MluCI AATT 5 cut(s) 233, 256, 441, 701, 778
MlyI GAGTC 3 cut(s) 115, 341, 365
MmeI TCCRAC 2 cut(s) 118, 309
MnlI CCTC 7 cut(s) 119, 309, 377, 471, 648, 785, 842
MroNI GCCGGC 1 cut(s) 104
MspA1I CMGCKG 2 cut(s) 536, 625
MspI CCGG 5 cut(s) 73, 93, 105, 220, 230
MspR9I CCNGG 3 cut(s) 221, 230, 747
MunI CAATTG 1 cut(s) 256
Mva1269I GAATGC 1 cut(s) 283
MvaI CCWGG 1 cut(s) 747
MwoI GCNNNNNNNGC 7 cut(s) 53, 62, 110, 252, 316, 539, 542
NaeI GCCGGC 1 cut(s) 106
NciI CCSGG 2 cut(s) 221, 230
NdeI CATATG 1 cut(s) 604
NdeII GATC 2 cut(s) 27, 454
NgoMIV GCCGGC 1 cut(s) 104
NlaIII CATG 1 cut(s) 121
NlaIV GGNNCC 2 cut(s) 154, 499
NmeAIII GCCGAG 1 cut(s) 23
PagI TCATGA 1 cut(s) 117
PctI GAATGC 1 cut(s) 283
PdiI GCCGGC 1 cut(s) 106
PfeI GAWTC 2 cut(s) 677, 711
PkrI GCNGC 7 cut(s) 58, 410, 535, 538, 541, 544, 624
PleI GAGTC 3 cut(s) 115, 341, 364
PpsI GAGTC 3 cut(s) 115, 341, 364
PpuMI RGGWCCY 1 cut(s) 749
Psp5II RGGWCCY 1 cut(s) 749
Psp6I CCWGG 1 cut(s) 745
PspGI CCWGG 1 cut(s) 745
PspN4I GGNNCC 2 cut(s) 154, 499
PspPI GGNCC 1 cut(s) 749
PspPPI RGGWCCY 1 cut(s) 749
PstI CTGCAG 1 cut(s) 544
PstNI CAGNNNCTG 2 cut(s) 539, 551
PvuII CAGCTG 2 cut(s) 536, 625
RsaI GTAC 3 cut(s) 499, 601, 718
RsaNI GTAC 3 cut(s) 498, 600, 717
SatI GCNGC 7 cut(s) 57, 409, 534, 537, 540, 543, 623
Sau3AI GATC 2 cut(s) 27, 454
Sau96I GGNCC 1 cut(s) 749
SchI GAGTC 3 cut(s) 115, 341, 365
ScrFI CCNGG 3 cut(s) 221, 230, 747
SfaNI GCATC 2 cut(s) 19, 329
SfcI CTRYAG 1 cut(s) 540
SfiI GGCCNNNNNGGCC 1 cut(s) 53
SinI GGWCC 1 cut(s) 749
Sse9I AATT 5 cut(s) 233, 256, 441, 701, 778
SsiI CCGC 5 cut(s) 57, 70, 246, 490, 507
StyD4I CCNGG 3 cut(s) 219, 228, 745
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 2 cut(s) 721, 757
TaiI ACGT 2 cut(s) 182, 858
TaqI TCGA 4 cut(s) 84, 185, 275, 350
TasI AATT 5 cut(s) 233, 256, 441, 701, 778
TatI WGTACW 2 cut(s) 599, 716
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 2 cut(s) 677, 711
TscAI CASTG 5 cut(s) 300, 381, 621, 702, 712
TseI GCWGC 6 cut(s) 408, 533, 536, 539, 542, 622
TspDTI ATGAA 1 cut(s) 279
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 5 cut(s) 300, 381, 621, 702, 712
VpaK11BI GGWCC 1 cut(s) 749
XapI RAATTY 2 cut(s) 233, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.