FvH4_1g04721

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010264
Physical Location & Seq
Forward (+)
2496599 .. 2499376
2778 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04721.t2

Sequence Viewer

Length: 1383 bp
ATGGTGTCAACAATTCTGTCATCAACCACATCCTCATTTCTTTCCAATTCCAACCCTTACTCTGATGCCTTTCCCACCTTCAACAAATCCAGACCACCCTTGGAACTTTCCAGAAGACCCACCCTCAACTTCCCCAAATCCCTCAAGCTTGTTCCTTTCAGGGTCTTCAGCTCTGCTGGCCCAATTGAGGATGGCTCAGCTGAGCAGTTTCTGCAGAACAATTCAATTGGGGACTTCATGAGGTTCAAGACTGGGGCAGATGGAGGTACTGGGGAGTTGCAGACTGCTGTTGTGAGTTACAAGAAGAAGTTCCCTTGGTCTCTTTTGAAGCCTTTTCTTCAGGTTGATTTGGTTTCGACGATTCATATAGCGGATAAAGAGTACTTCTCAACCCTCCAAAAGGAGCTTGAGTCCTATGATGCTGTTCTTTTTGAGATGGTGACTAGCAAAGAGAGTTTAGAGAATAGAAGAAACCTTGTTGCCACGAAAAGACTCAAAGTCAAAAGTTCACGCTCAAGAAGCTTTAACATTCTGGGATGCATTCAGCGGCAGATGGCTCATTTTCTGAGTCTTGATTTCCAGCTAGACTGTATGGACTATGAGTCTGAGAAATGGTATCATGCAGATCTCGATTTTGAGACTTTCAAGTTGCTTCAGGATGAAAAAGGCGAAAGCTTCTTCTCCTTTGCAAGGGATATGACAATTAGATCCACGAAAGCTATGATGAAACCTGAAATTCCAGAAGACCTTGATCCTTGGAGATCTAAGCTTTTATGGGCCTCACGTGTTCTTCCTATGCCACTTGTTGGCCTCCTCATTATTGGAGGTGTTTGTGCAGACACGGGCAGTCAGCCATCAAAATTTCCAGAACTAGAGGCCTTATCCAGGCTTGATTTTGGTGGTGCAATGAAGGTCTTCTTAGCCAAAAGGTTAACATCTGAATTCACGCTGGCGACAGCTGATGTAGAAGAGAGTTCTGTGATCATTGGTGAAAGAAACAGAGCTTGCATGGAAGCTCTCACAAAAGCAATTGACGATGGGCACAATAGGATTGCAATACTATACGGAGGTGGTCACATGCCTGATCTAGGAAGGAGATTACAAGAGTTGGATCTGATCCCTTCTCGGGCACAGTGGGTAACGGCGTGGTCCATAAAGAACAGGAACTTAAACAGCAATTCCCTTCCATTTCTGAAGACAATGGCTGAAGTTTCGGGTTGGCCTTTGAATCGCTACCAGACTTTGGCATTGCTTATATTTTCATCAGTGCTCGCAATAGATCTCTGGTTCTGGGAGCTCTTTTTTGGCACTGCTGTCGACTTTTTCTCTCAAATTGGTTCACAGGTTTCGCAGTTTATTGATAATGCAGATATGATATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

461

Amino Acids

51.97

Weight (kDa)

6.07

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010264)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19540 AT5G19540
fragaria_vesca FvH4_1g04721 FvH4_1g04721 FvH4_1g04721
malus_domestica MD02G1046200.v1.1 MD15G1184300.v1.1
prunus_persica Prupe.7G231800_v2.0.a1
pyrus_communis pycom02g03870 pycom15g16590
rosa_chinensis RchiOBHm_Chr2g0090331
rosa_laevigata RLG00000016099
rosa_multiflora Rmu_sc0006235.1_g000006
rosa_roxburghii Rroxscaffold_2G00151270
rosa_rugosa Rorug02G0006600 Rorug02G0006600
rosa_samantha Rh2AG052600 Rh2BG051200 Rh2CG053400 Rh2DG052500
rosa_wichuraiana Rw2G004430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1381
AccB7I CCANNNNNTGG 2 cut(s) 806, 1243
AccI GTMKAC 1 cut(s) 1317
AciI CCGC 2 cut(s) 371, 547
AclWI GGATC 4 cut(s) 702, 746, 1111, 1119
AcsI RAATTY 3 cut(s) 735, 860, 941
AcuI CTGAAG 5 cut(s) 151, 323, 638, 1214, 1227
AcvI CACGTG 1 cut(s) 785
AfaI GTAC 2 cut(s) 268, 383
AfiI CCNNNNNNNGG 8 cut(s) 187, 400, 690, 806, 885, 1088, 1126, 1243
AflIII ACRYGT 1 cut(s) 784
AgsI TTSAA 6 cut(s) 82, 225, 247, 328, 646, 1228
AhdI GACNNNNNGTC 2 cut(s) 497, 601
AjnI CCWGG 1 cut(s) 884
Alw21I GWGCWC 2 cut(s) 1272, 1299
Alw26I GTCTC 2 cut(s) 324, 632
AlwI GGATC 4 cut(s) 702, 746, 1111, 1119
AlwNI CAGNNNCTG 1 cut(s) 211
Ama87I CYCGRG 1 cut(s) 1125
AoxI GGCC 5 cut(s) 178, 777, 808, 876, 1220
ApoI RAATTY 3 cut(s) 735, 860, 941
Asp700I GAANNNNTTC 2 cut(s) 308, 914
AspS9I GGNCC 3 cut(s) 179, 777, 1149
AsuHPI GGTGA 2 cut(s) 451, 1001
AvaI CYCGRG 1 cut(s) 1125
AvaII GGWCC 1 cut(s) 1149
BaeGI GKGCMC 2 cut(s) 1044, 1132
BanII GRGCYC 1 cut(s) 1299
BbrPI CACGTG 1 cut(s) 785
BbsI GAAGAC 5 cut(s) 121, 157, 750, 907, 1202
Bbv12I GWGCWC 2 cut(s) 1272, 1299
BccI CCATC 6 cut(s) 185, 254, 430, 547, 862, 1031
BceAI ACGGC 1 cut(s) 1158
BcgI CGANNNNNNTGC 2 cut(s) 1297, 1331
BciT130I CCWGG 1 cut(s) 886
BclI TGATCA 1 cut(s) 981
BcoDI GTCTC 2 cut(s) 324, 632
BfaI CTAG 4 cut(s) 444, 584, 872, 1088
BfmI CTRYAG 1 cut(s) 212
BglII AGATCT 3 cut(s) 625, 761, 1279
BisI GCNGC 1 cut(s) 548
BlpI GCTNAGC 2 cut(s) 196, 201
BlsI GCNGC 1 cut(s) 549
BmcAI AGTACT 1 cut(s) 383
Bme1390I CCNGG 1 cut(s) 886
Bme18I GGWCC 1 cut(s) 1149
BmeRI GACNNNNNGTC 2 cut(s) 497, 601
BmeT110I CYCGRG 1 cut(s) 1125
BmgT120I GGNCC 3 cut(s) 179, 777, 1149
BmrFI CCNGG 1 cut(s) 886
BmrI ACTGGG 2 cut(s) 261, 279
BmsI GCATC 3 cut(s) 55, 409, 527
BmuI ACTGGG 2 cut(s) 261, 279
BpiI GAAGAC 5 cut(s) 121, 157, 750, 907, 1202
BplI GAGNNNNNCTC 4 cut(s) 179, 211, 371, 403
Bpu1102I GCTNAGC 2 cut(s) 196, 201
BpuEI CTTGAG 3 cut(s) 128, 428, 499
BsaAI YACGTR 1 cut(s) 785
BsaI GGTCTC 1 cut(s) 324
BsaJI CCNNGG 3 cut(s) 99, 314, 755
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 8 cut(s) 187, 400, 690, 806, 885, 1088, 1126, 1243
Bse1I ACTGG 2 cut(s) 256, 274
Bse3DI GCAATG 2 cut(s) 912, 1247
BseBI CCWGG 1 cut(s) 886
BseDI CCNNGG 3 cut(s) 99, 314, 755
BseGI GGATG 4 cut(s) 29, 196, 542, 664
BseLI CCNNNNNNNGG 8 cut(s) 187, 400, 690, 806, 885, 1088, 1126, 1243
BseMI GCAATG 2 cut(s) 912, 1247
BseMII CTCAG 4 cut(s) 192, 210, 557, 597
BseNI ACTGG 2 cut(s) 256, 274
BseRI GAGGAG 1 cut(s) 803
BseSI GKGCMC 2 cut(s) 1044, 1132
BsgI GTGCAG 1 cut(s) 855
BshFI GGCC 5 cut(s) 180, 779, 810, 878, 1222
BsiHKAI GWGCWC 2 cut(s) 1272, 1299
BsiHKCI CYCGRG 1 cut(s) 1125
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 8 cut(s) 187, 400, 690, 806, 885, 1088, 1126, 1243
BsmAI GTCTC 2 cut(s) 324, 632
BsmFI GGGAC 1 cut(s) 245
BsmI GAATGC 1 cut(s) 540
BsnI GGCC 5 cut(s) 180, 779, 810, 878, 1222
Bso31I GGTCTC 1 cut(s) 324
BsoBI CYCGRG 1 cut(s) 1125
Bsp1286I GDGCHC 4 cut(s) 1044, 1132, 1272, 1299
Bsp143I GATC 9 cut(s) 625, 707, 751, 761, 981, 1084, 1111, 1116, 1279
Bsp1720I GCTNAGC 2 cut(s) 196, 201
BspACI CCGC 2 cut(s) 371, 547
BspANI GGCC 5 cut(s) 180, 779, 810, 878, 1222
BspCNI CTCAG 4 cut(s) 193, 209, 558, 598
BspHI TCATGA 1 cut(s) 237
BspMAI CTGCAG 1 cut(s) 216
BspPI GGATC 4 cut(s) 702, 746, 1111, 1119
BspTNI GGTCTC 1 cut(s) 324
BsrDI GCAATG 2 cut(s) 912, 1247
BsrI ACTGG 2 cut(s) 256, 274
BssECI CCNNGG 3 cut(s) 99, 314, 755
BssMI GATC 9 cut(s) 625, 707, 751, 761, 981, 1084, 1111, 1116, 1279
BssT1I CCWWGG 3 cut(s) 99, 314, 755
Bst2UI CCWGG 1 cut(s) 886
Bst4CI ACNGT 2 cut(s) 590, 1134
Bst6I CTCTTC 1 cut(s) 963
BstAPI GCANNNNNTGC 1 cut(s) 211
BstBAI YACGTR 1 cut(s) 785
BstC8I GCNNGC 4 cut(s) 178, 951, 1006, 1272
BstDEI CTNAG 6 cut(s) 196, 201, 566, 606, 765, 919
BstENI CCTNNNNNAGG 4 cut(s) 398, 688, 883, 1086
BstF5I GGATG 4 cut(s) 29, 196, 542, 664
BstKTI GATC 9 cut(s) 628, 710, 754, 764, 984, 1087, 1114, 1119, 1282
BstMAI GTCTC 2 cut(s) 324, 632
BstMBI GATC 9 cut(s) 625, 707, 751, 761, 981, 1084, 1111, 1116, 1279
BstMWI GCNNNNNNNGC 3 cut(s) 177, 211, 519
BstNI CCWGG 1 cut(s) 886
BstNSI RCATGY 1 cut(s) 1081
BstSCI CCNGG 1 cut(s) 884
BstSFI CTRYAG 1 cut(s) 212
BstSLI GKGCMC 2 cut(s) 1044, 1132
BstV2I GAAGAC 5 cut(s) 121, 157, 750, 907, 1202
BstX2I RGATCY 5 cut(s) 625, 707, 761, 1111, 1279
BstYI RGATCY 5 cut(s) 625, 707, 761, 1111, 1279
BsuRI GGCC 5 cut(s) 180, 779, 810, 878, 1222
BtsCI GGATG 4 cut(s) 29, 196, 542, 664
BtsI GCAGTG 1 cut(s) 1308
BtsIMutI CAGTG 3 cut(s) 1139, 1272, 1308
Cac8I GCNNGC 4 cut(s) 178, 951, 1006, 1272
CaiI CAGNNNCTG 1 cut(s) 211
CciI TCATGA 1 cut(s) 237
Cfr13I GGNCC 3 cut(s) 179, 777, 1149
Csp6I GTAC 2 cut(s) 267, 382
CviAII CATG 4 cut(s) 238, 620, 1009, 1078
CviQI GTAC 2 cut(s) 267, 382
DdeI CTNAG 6 cut(s) 196, 201, 566, 606, 765, 919
DpnI GATC 9 cut(s) 627, 709, 753, 763, 983, 1086, 1113, 1118, 1281
DpnII GATC 9 cut(s) 625, 707, 751, 761, 981, 1084, 1111, 1116, 1279
DriI GACNNNNNGTC 2 cut(s) 497, 601
Eam1104I CTCTTC 1 cut(s) 963
Eam1105I GACNNNNNGTC 2 cut(s) 497, 601
EarI CTCTTC 1 cut(s) 963
Ecl136II GAGCTC 1 cut(s) 1297
Eco130I CCWWGG 3 cut(s) 99, 314, 755
Eco147I AGGCCT 1 cut(s) 878
Eco24I GRGCYC 1 cut(s) 1299
Eco31I GGTCTC 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 1149
Eco53kI GAGCTC 1 cut(s) 1297
Eco57I CTGAAG 5 cut(s) 151, 323, 638, 1214, 1227
Eco72I CACGTG 1 cut(s) 785
Eco88I CYCGRG 1 cut(s) 1125
EcoICRI GAGCTC 1 cut(s) 1297
EcoNI CCTNNNNNAGG 4 cut(s) 398, 688, 883, 1086
EcoRI GAATTC 1 cut(s) 941
EcoRII CCWGG 1 cut(s) 884
EcoT14I CCWWGG 3 cut(s) 99, 314, 755
EcoT22I ATGCAT 1 cut(s) 542
EcoT38I GRGCYC 1 cut(s) 1299
ErhI CCWWGG 3 cut(s) 99, 314, 755
FaeI CATG 4 cut(s) 241, 623, 1012, 1081
FalI AAGNNNNNCTT 2 cut(s) 902, 934
FaqI GGGAC 1 cut(s) 245
FatI CATG 4 cut(s) 237, 619, 1008, 1077
FbaI TGATCA 1 cut(s) 981
FblI GTMKAC 1 cut(s) 1317
Fnu4HI GCNGC 1 cut(s) 548
FokI GGATG 4 cut(s) 16, 203, 549, 671
FriOI GRGCYC 1 cut(s) 1299
Fsp4HI GCNGC 1 cut(s) 548
FspBI CTAG 4 cut(s) 444, 584, 872, 1088
GluI GCNGC 1 cut(s) 548
HaeIII GGCC 5 cut(s) 180, 779, 810, 878, 1222
Hin1II CATG 4 cut(s) 241, 623, 1012, 1081
HincII GTYRAC 3 cut(s) 9, 933, 1318
HindII GTYRAC 3 cut(s) 9, 933, 1318
HindIII AAGCTT 4 cut(s) 146, 520, 673, 767
HinfI GANTC 6 cut(s) 361, 410, 492, 568, 602, 1228
HpaI GTTAAC 1 cut(s) 933
HphI GGTGA 2 cut(s) 451, 1001
Hpy166II GTNNAC 5 cut(s) 9, 509, 933, 1318, 1340
Hpy188I TCNGA 6 cut(s) 64, 567, 607, 940, 1116, 1194
Hpy8I GTNNAC 5 cut(s) 9, 509, 933, 1318, 1340
Hpy99I CGWCG 1 cut(s) 361
HpyAV CCTTC 5 cut(s) 88, 904, 1086, 1131, 1193
HpyCH4III ACNGT 2 cut(s) 590, 1134
HpyCH4IV ACGT 1 cut(s) 784
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 211, 519
HpyF3I CTNAG 6 cut(s) 196, 201, 566, 606, 765, 919
HpySE526I ACGT 1 cut(s) 784
Hsp92II CATG 4 cut(s) 241, 623, 1012, 1081
Ksp22I TGATCA 1 cut(s) 981
KspAI GTTAAC 1 cut(s) 933
Kzo9I GATC 9 cut(s) 625, 707, 751, 761, 981, 1084, 1111, 1116, 1279
LmnI GCTCC 2 cut(s) 403, 1294
LweI GCATC 3 cut(s) 55, 409, 527
MaeI CTAG 4 cut(s) 444, 584, 872, 1088
MaeII ACGT 1 cut(s) 784
MaeIII GTNAC 4 cut(s) 296, 439, 1073, 1138
MalI GATC 9 cut(s) 627, 709, 753, 763, 983, 1086, 1113, 1118, 1281
MboI GATC 9 cut(s) 625, 707, 751, 761, 981, 1084, 1111, 1116, 1279
MfeI CAATTG 3 cut(s) 183, 225, 1029
MflI RGATCY 5 cut(s) 625, 707, 761, 1111, 1279
MhlI GDGCHC 4 cut(s) 1044, 1132, 1272, 1299
MlyI GAGTC 4 cut(s) 419, 486, 577, 611
MmeI TCCRAC 2 cut(s) 75, 1089
Mph1103I ATGCAT 1 cut(s) 542
MroXI GAANNNNTTC 2 cut(s) 308, 914
MseI TTAA 3 cut(s) 525, 932, 1169
MspA1I CMGCKG 3 cut(s) 200, 547, 959
MspR9I CCNGG 1 cut(s) 886
MunI CAATTG 3 cut(s) 183, 225, 1029
Mva1269I GAATGC 1 cut(s) 540
MvaI CCWGG 1 cut(s) 886
MwoI GCNNNNNNNGC 3 cut(s) 177, 211, 519
NdeII GATC 9 cut(s) 625, 707, 751, 761, 981, 1084, 1111, 1116, 1279
NlaIII CATG 4 cut(s) 241, 623, 1012, 1081
NmuCI GTSAC 2 cut(s) 439, 1073
NsiI ATGCAT 1 cut(s) 542
NspI RCATGY 1 cut(s) 1081
PagI TCATGA 1 cut(s) 237
PceI AGGCCT 1 cut(s) 878
PctI GAATGC 1 cut(s) 540
PdmI GAANNNNTTC 2 cut(s) 308, 914
PfeI GAWTC 2 cut(s) 361, 1228
PflMI CCANNNNNTGG 2 cut(s) 806, 1243
PkrI GCNGC 1 cut(s) 549
PleI GAGTC 4 cut(s) 418, 486, 576, 610
PmaCI CACGTG 1 cut(s) 785
PmlI CACGTG 1 cut(s) 785
PpsI GAGTC 4 cut(s) 418, 486, 576, 610
Ppu21I YACGTR 1 cut(s) 785
PsiI TTATAA 1 cut(s) 1381
Psp124BI GAGCTC 1 cut(s) 1299
Psp6I CCWGG 1 cut(s) 884
PspCI CACGTG 1 cut(s) 785
PspGI CCWGG 1 cut(s) 884
PspPI GGNCC 3 cut(s) 179, 777, 1149
PstI CTGCAG 1 cut(s) 216
PstNI CAGNNNCTG 1 cut(s) 211
PsuI RGATCY 5 cut(s) 625, 707, 761, 1111, 1279
PvuII CAGCTG 2 cut(s) 200, 959
RsaI GTAC 2 cut(s) 268, 383
RsaNI GTAC 2 cut(s) 267, 382
SacI GAGCTC 1 cut(s) 1299
SalI GTCGAC 1 cut(s) 1316
SaqAI TTAA 3 cut(s) 525, 932, 1169
SatI GCNGC 1 cut(s) 548
Sau3AI GATC 9 cut(s) 625, 707, 751, 761, 981, 1084, 1111, 1116, 1279
Sau96I GGNCC 3 cut(s) 179, 777, 1149
ScaI AGTACT 1 cut(s) 383
SchI GAGTC 4 cut(s) 419, 486, 577, 611
ScrFI CCNGG 1 cut(s) 886
SduI GDGCHC 4 cut(s) 1044, 1132, 1272, 1299
SfaNI GCATC 3 cut(s) 55, 409, 527
SfcI CTRYAG 1 cut(s) 212
SinI GGWCC 1 cut(s) 1149
SmlI CTYRAG 3 cut(s) 143, 407, 514
SmoI CTYRAG 3 cut(s) 143, 407, 514
SseBI AGGCCT 1 cut(s) 878
SsiI CCGC 2 cut(s) 371, 547
SspMI CTAG 4 cut(s) 444, 584, 872, 1088
SstI GAGCTC 1 cut(s) 1299
StuI AGGCCT 1 cut(s) 878
StyD4I CCNGG 1 cut(s) 884
StyI CCWWGG 3 cut(s) 99, 314, 755
TaaI ACNGT 2 cut(s) 590, 1134
TaiI ACGT 1 cut(s) 787
TaqI TCGA 3 cut(s) 356, 630, 1317
TatI WGTACW 1 cut(s) 381
TauI GCSGC 1 cut(s) 550
TfiI GAWTC 2 cut(s) 361, 1228
Tru1I TTAA 3 cut(s) 525, 932, 1169
Tru9I TTAA 3 cut(s) 525, 932, 1169
TscAI CASTG 3 cut(s) 1139, 1272, 1315
TseFI GTSAC 2 cut(s) 439, 1073
Tsp45I GTSAC 2 cut(s) 439, 1073
TspDTI ATGAA 6 cut(s) 226, 353, 675, 740, 923, 1251
TspGWI ACGGA 1 cut(s) 1080
TspRI CASTG 3 cut(s) 1139, 1272, 1315
Van91I CCANNNNNTGG 2 cut(s) 806, 1243
VpaK11BI GGWCC 1 cut(s) 1149
XagI CCTNNNNNAGG 4 cut(s) 398, 688, 883, 1086
XapI RAATTY 3 cut(s) 735, 860, 941
XceI RCATGY 1 cut(s) 1081
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XmiI GTMKAC 1 cut(s) 1317
XmnI GAANNNNTTC 2 cut(s) 308, 914
XspI CTAG 4 cut(s) 444, 584, 872, 1088
ZrmI AGTACT 1 cut(s) 383
Zsp2I ATGCAT 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.