Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G56120 |
| fragaria_vesca | FvH4_1g05380 |
| malus_domestica | MD02G1062400.v1.1 |
| pyrus_communis | pycom02g04360 |
| rosa_chinensis | RchiOBHm_Chr2g0091071 |
| rosa_laevigata | RLG00000016174 |
| rosa_multiflora | Rmu_sc0005171.1_g000009 |
| rosa_roxburghii | Rroxscaffold_2G00150620 |
| rosa_rugosa | Rorug02G0012300 |
| rosa_samantha | Rh2AG058400 Rh2BG056800 Rh2CG059000 Rh2DG058300 |
| rosa_wichuraiana | Rw2G005080 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 287, 1081 |
| Acc65I | GGTACC | 1 cut(s) | 988 |
| AccB1I | GGYRCC | 1 cut(s) | 988 |
| AccIII | TCCGGA | 2 cut(s) | 208, 751 |
| AciI | CCGC | 1 cut(s) | 711 |
| AclWI | GGATC | 3 cut(s) | 243, 493, 1264 |
| AcsI | RAATTY | 6 cut(s) | 17, 500, 607, 836, 883, 1244 |
| AcuI | CTGAAG | 2 cut(s) | 251, 1035 |
| AdeI | CACNNNGTG | 1 cut(s) | 1411 |
| AfaI | GTAC | 5 cut(s) | 87, 281, 922, 990, 1056 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 365, 869 |
| AflII | CTTAAG | 1 cut(s) | 1447 |
| AflIII | ACRYGT | 1 cut(s) | 917 |
| AhlI | ACTAGT | 1 cut(s) | 143 |
| AjiI | CACGTC | 1 cut(s) | 25 |
| AjnI | CCWGG | 1 cut(s) | 359 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 599, 631 |
| AluBI | AGCT | 6 cut(s) | 64, 326, 583, 594, 1064, 1452 |
| AluI | AGCT | 6 cut(s) | 64, 326, 583, 594, 1064, 1452 |
| Alw21I | GWGCWC | 1 cut(s) | 66 |
| Alw26I | GTCTC | 1 cut(s) | 908 |
| AlwI | GGATC | 3 cut(s) | 243, 493, 1264 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 863, 1370 |
| Ama87I | CYCGRG | 1 cut(s) | 81 |
| Aor13HI | TCCGGA | 2 cut(s) | 208, 751 |
| AoxI | GGCC | 2 cut(s) | 691, 1380 |
| ApeKI | GCWGC | 2 cut(s) | 1370, 1459 |
| ApoI | RAATTY | 6 cut(s) | 17, 500, 607, 836, 883, 1244 |
| Asp718I | GGTACC | 1 cut(s) | 988 |
| AspLEI | GCGC | 1 cut(s) | 46 |
| AspS9I | GGNCC | 3 cut(s) | 136, 181, 692 |
| AsuC2I | CCSGG | 1 cut(s) | 1229 |
| AsuHPI | GGTGA | 3 cut(s) | 812, 1279, 1423 |
| AvaI | CYCGRG | 1 cut(s) | 81 |
| AvaII | GGWCC | 2 cut(s) | 136, 181 |
| BanI | GGYRCC | 1 cut(s) | 988 |
| BanII | GRGCYC | 1 cut(s) | 66 |
| BauI | CACGAG | 2 cut(s) | 57, 114 |
| Bbv12I | GWGCWC | 1 cut(s) | 66 |
| BbvI | GCAGC | 2 cut(s) | 1357, 1446 |
| BccI | CCATC | 3 cut(s) | 390, 847, 1103 |
| BciT130I | CCWGG | 1 cut(s) | 361 |
| BcnI | CCSGG | 1 cut(s) | 1229 |
| BcoDI | GTCTC | 1 cut(s) | 908 |
| BcuI | ACTAGT | 1 cut(s) | 143 |
| BfaI | CTAG | 4 cut(s) | 144, 275, 1032, 1250 |
| BfoI | RGCGCY | 1 cut(s) | 47 |
| BfrI | CTTAAG | 1 cut(s) | 1447 |
| BfuAI | ACCTGC | 2 cut(s) | 287, 1081 |
| BisI | GCNGC | 3 cut(s) | 711, 1371, 1460 |
| BlsI | GCNGC | 3 cut(s) | 712, 1372, 1461 |
| BmcAI | AGTACT | 1 cut(s) | 87 |
| Bme1390I | CCNGG | 2 cut(s) | 361, 1229 |
| Bme18I | GGWCC | 2 cut(s) | 136, 181 |
| BmeT110I | CYCGRG | 1 cut(s) | 81 |
| BmgBI | CACGTC | 1 cut(s) | 25 |
| BmgT120I | GGNCC | 3 cut(s) | 136, 181, 692 |
| BmiI | GGNNCC | 2 cut(s) | 138, 990 |
| BmrFI | CCNGG | 2 cut(s) | 361, 1229 |
| BmsI | GCATC | 7 cut(s) | 713, 817, 880, 954, 956, 1036, 1243 |
| BoxI | GACNNNNGTC | 1 cut(s) | 470 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1279, 1311 |
| BpmI | CTGGAG | 2 cut(s) | 382, 1044 |
| BpuEI | CTTGAG | 1 cut(s) | 887 |
| BpuMI | CCSGG | 1 cut(s) | 1229 |
| BsaJI | CCNNGG | 1 cut(s) | 359 |
| BsaWI | WCCGGW | 2 cut(s) | 208, 751 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 355, 360, 385, 390, 525, 555 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 365, 869 |
| Bse1I | ACTGG | 1 cut(s) | 1135 |
| Bse3DI | GCAATG | 2 cut(s) | 961, 1443 |
| BseAI | TCCGGA | 2 cut(s) | 208, 751 |
| BseBI | CCWGG | 1 cut(s) | 361 |
| BseDI | CCNNGG | 1 cut(s) | 359 |
| BseGI | GGATG | 4 cut(s) | 424, 728, 1323, 1423 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 365, 869 |
| BseMI | GCAATG | 2 cut(s) | 961, 1443 |
| BseMII | CTCAG | 4 cut(s) | 235, 659, 1164, 1371 |
| BseNI | ACTGG | 1 cut(s) | 1135 |
| BseRI | GAGGAG | 1 cut(s) | 1291 |
| BseXI | GCAGC | 2 cut(s) | 1357, 1446 |
| BsgI | GTGCAG | 1 cut(s) | 315 |
| BshFI | GGCC | 2 cut(s) | 693, 1382 |
| BshNI | GGYRCC | 1 cut(s) | 988 |
| BsiHKAI | GWGCWC | 1 cut(s) | 66 |
| BsiHKCI | CYCGRG | 1 cut(s) | 81 |
| BsiSI | CCGG | 3 cut(s) | 209, 752, 1229 |
| BslFI | GGGAC | 1 cut(s) | 1304 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 365, 869 |
| BsmAI | GTCTC | 1 cut(s) | 908 |
| BsmFI | GGGAC | 1 cut(s) | 1304 |
| BsmI | GAATGC | 1 cut(s) | 759 |
| BsnI | GGCC | 2 cut(s) | 693, 1382 |
| BsoBI | CYCGRG | 1 cut(s) | 81 |
| Bsp1286I | GDGCHC | 1 cut(s) | 66 |
| Bsp13I | TCCGGA | 2 cut(s) | 208, 751 |
| Bsp143I | GATC | 7 cut(s) | 235, 485, 774, 1018, 1176, 1207, 1269 |
| BspACI | CCGC | 1 cut(s) | 711 |
| BspANI | GGCC | 2 cut(s) | 693, 1382 |
| BspCNI | CTCAG | 4 cut(s) | 234, 658, 1165, 1370 |
| BspEI | TCCGGA | 2 cut(s) | 208, 751 |
| BspLI | GGNNCC | 2 cut(s) | 138, 990 |
| BspMI | ACCTGC | 2 cut(s) | 287, 1081 |
| BspPI | GGATC | 3 cut(s) | 243, 493, 1264 |
| BspT107I | GGYRCC | 1 cut(s) | 988 |
| BspTI | CTTAAG | 1 cut(s) | 1447 |
| BsrDI | GCAATG | 2 cut(s) | 961, 1443 |
| BsrI | ACTGG | 1 cut(s) | 1135 |
| BssECI | CCNNGG | 1 cut(s) | 359 |
| BssMI | GATC | 7 cut(s) | 235, 485, 774, 1018, 1176, 1207, 1269 |
| BssSI | CACGAG | 2 cut(s) | 57, 114 |
| Bst2BI | CACGAG | 2 cut(s) | 57, 114 |
| Bst2UI | CCWGG | 1 cut(s) | 361 |
| Bst4CI | ACNGT | 4 cut(s) | 158, 869, 973, 1121 |
| Bst6I | CTCTTC | 2 cut(s) | 1096, 1302 |
| BstAFI | CTTAAG | 1 cut(s) | 1447 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1370 |
| BstC8I | GCNNGC | 1 cut(s) | 1471 |
| BstDEI | CTNAG | 5 cut(s) | 221, 327, 645, 1173, 1357 |
| BstEII | GGTNACC | 1 cut(s) | 800 |
| BstF5I | GGATG | 4 cut(s) | 424, 728, 1323, 1423 |
| BstH2I | RGCGCY | 1 cut(s) | 47 |
| BstHHI | GCGC | 1 cut(s) | 46 |
| BstKTI | GATC | 7 cut(s) | 238, 488, 777, 1021, 1179, 1210, 1272 |
| BstMAI | GTCTC | 1 cut(s) | 908 |
| BstMBI | GATC | 7 cut(s) | 235, 485, 774, 1018, 1176, 1207, 1269 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 707, 1146, 1370, 1379 |
| BstNI | CCWGG | 1 cut(s) | 361 |
| BstNSI | RCATGY | 3 cut(s) | 921, 1205, 1473 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 470 |
| BstPI | GGTNACC | 1 cut(s) | 800 |
| BstSCI | CCNGG | 2 cut(s) | 359, 1227 |
| BstV1I | GCAGC | 2 cut(s) | 1357, 1446 |
| BstX2I | RGATCY | 2 cut(s) | 235, 485 |
| BstYI | RGATCY | 2 cut(s) | 235, 485 |
| BsuRI | GGCC | 2 cut(s) | 693, 1382 |
| BtrI | CACGTC | 1 cut(s) | 25 |
| BtsCI | GGATG | 4 cut(s) | 424, 728, 1323, 1423 |
| BtsI | GCAGTG | 2 cut(s) | 864, 1309 |
| BtsIMutI | CAGTG | 5 cut(s) | 229, 864, 874, 1309, 1365 |
| BveI | ACCTGC | 2 cut(s) | 287, 1081 |
| Cac8I | GCNNGC | 1 cut(s) | 1471 |
| CaiI | CAGNNNCTG | 2 cut(s) | 863, 1370 |
| CfoI | GCGC | 1 cut(s) | 46 |
| Cfr13I | GGNCC | 3 cut(s) | 136, 181, 692 |
| Csp6I | GTAC | 5 cut(s) | 86, 280, 921, 989, 1055 |
| CviQI | GTAC | 5 cut(s) | 86, 280, 921, 989, 1055 |
| DdeI | CTNAG | 5 cut(s) | 221, 327, 645, 1173, 1357 |
| DpnI | GATC | 7 cut(s) | 237, 487, 776, 1020, 1178, 1209, 1271 |
| DpnII | GATC | 7 cut(s) | 235, 485, 774, 1018, 1176, 1207, 1269 |
| DraIII | CACNNNGTG | 1 cut(s) | 1411 |
| Eam1104I | CTCTTC | 2 cut(s) | 1096, 1302 |
| EarI | CTCTTC | 2 cut(s) | 1096, 1302 |
| Ecl136II | GAGCTC | 1 cut(s) | 64 |
| Eco24I | GRGCYC | 1 cut(s) | 66 |
| Eco47I | GGWCC | 2 cut(s) | 136, 181 |
| Eco53kI | GAGCTC | 1 cut(s) | 64 |
| Eco57I | CTGAAG | 2 cut(s) | 251, 1035 |
| Eco88I | CYCGRG | 1 cut(s) | 81 |
| Eco91I | GGTNACC | 1 cut(s) | 800 |
| EcoICRI | GAGCTC | 1 cut(s) | 64 |
| EcoO109I | RGGNCCY | 1 cut(s) | 136 |
| EcoO65I | GGTNACC | 1 cut(s) | 800 |
| EcoRI | GAATTC | 2 cut(s) | 607, 883 |
| EcoRII | CCWGG | 1 cut(s) | 359 |
| EcoT22I | ATGCAT | 2 cut(s) | 728, 1258 |
| EcoT38I | GRGCYC | 1 cut(s) | 66 |
| FaqI | GGGAC | 1 cut(s) | 1304 |
| FauNDI | CATATG | 1 cut(s) | 668 |
| Fnu4HI | GCNGC | 3 cut(s) | 711, 1371, 1460 |
| FokI | GGATG | 4 cut(s) | 431, 735, 1310, 1430 |
| FriOI | GRGCYC | 1 cut(s) | 66 |
| Fsp4HI | GCNGC | 3 cut(s) | 711, 1371, 1460 |
| FspBI | CTAG | 4 cut(s) | 144, 275, 1032, 1250 |
| GlaI | GCGC | 1 cut(s) | 45 |
| GluI | GCNGC | 3 cut(s) | 711, 1371, 1460 |
| GsuI | CTGGAG | 2 cut(s) | 382, 1044 |
| HaeII | RGCGCY | 1 cut(s) | 47 |
| HaeIII | GGCC | 2 cut(s) | 693, 1382 |
| HapII | CCGG | 3 cut(s) | 209, 752, 1229 |
| HhaI | GCGC | 1 cut(s) | 46 |
| Hin6I | GCGC | 1 cut(s) | 44 |
| HinP1I | GCGC | 1 cut(s) | 44 |
| HincII | GTYRAC | 1 cut(s) | 270 |
| HindII | GTYRAC | 1 cut(s) | 270 |
| HindIII | AAGCTT | 2 cut(s) | 581, 1450 |
| HinfI | GANTC | 7 cut(s) | 52, 188, 350, 459, 941, 1222, 1306 |
| HpaII | CCGG | 3 cut(s) | 209, 752, 1229 |
| HphI | GGTGA | 3 cut(s) | 812, 1279, 1423 |
| Hpy166II | GTNNAC | 4 cut(s) | 227, 270, 811, 1015 |
| Hpy188I | TCNGA | 9 cut(s) | 51, 175, 193, 387, 889, 1009, 1093, 1174, 1328 |
| Hpy8I | GTNNAC | 4 cut(s) | 227, 270, 811, 1015 |
| Hpy99I | CGWCG | 1 cut(s) | 977 |
| HpyAV | CCTTC | 3 cut(s) | 172, 226, 1372 |
| HpyCH4III | ACNGT | 4 cut(s) | 158, 869, 973, 1121 |
| HpyCH4IV | ACGT | 2 cut(s) | 24, 375 |
| HpyCH4V | TGCA | 8 cut(s) | 28, 69, 296, 726, 737, 782, 1076, 1256 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 707, 1146, 1370, 1379 |
| HpyF3I | CTNAG | 5 cut(s) | 221, 327, 645, 1173, 1357 |
| HpySE526I | ACGT | 2 cut(s) | 24, 375 |
| HspAI | GCGC | 1 cut(s) | 44 |
| Kpn2I | TCCGGA | 2 cut(s) | 208, 751 |
| KpnI | GGTACC | 1 cut(s) | 992 |
| Kzo9I | GATC | 7 cut(s) | 235, 485, 774, 1018, 1176, 1207, 1269 |
| LmnI | GCTCC | 4 cut(s) | 569, 1137, 1198, 1429 |
| Lsp1109I | GCAGC | 2 cut(s) | 1357, 1446 |
| LweI | GCATC | 7 cut(s) | 713, 817, 880, 954, 956, 1036, 1243 |
| MaeI | CTAG | 4 cut(s) | 144, 275, 1032, 1250 |
| MaeII | ACGT | 2 cut(s) | 24, 375 |
| MaeIII | GTNAC | 7 cut(s) | 158, 330, 371, 547, 800, 1000, 1126 |
| MalI | GATC | 7 cut(s) | 237, 487, 776, 1020, 1178, 1209, 1271 |
| MboI | GATC | 7 cut(s) | 235, 485, 774, 1018, 1176, 1207, 1269 |
| MboII | GAAGA | 4 cut(s) | 345, 784, 1113, 1289 |
| MfeI | CAATTG | 2 cut(s) | 468, 848 |
| MflI | RGATCY | 2 cut(s) | 235, 485 |
| MhlI | GDGCHC | 1 cut(s) | 66 |
| MmeI | TCCRAC | 2 cut(s) | 216, 463 |
| Mph1103I | ATGCAT | 2 cut(s) | 728, 1258 |
| MroI | TCCGGA | 2 cut(s) | 208, 751 |
| MseI | TTAA | 7 cut(s) | 120, 404, 746, 789, 1188, 1448, 1480 |
| MspCI | CTTAAG | 1 cut(s) | 1447 |
| MspI | CCGG | 3 cut(s) | 209, 752, 1229 |
| MspR9I | CCNGG | 2 cut(s) | 361, 1229 |
| MunI | CAATTG | 2 cut(s) | 468, 848 |
| Mva1269I | GAATGC | 1 cut(s) | 759 |
| MvaI | CCWGG | 1 cut(s) | 361 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 707, 1146, 1370, 1379 |
| NciI | CCSGG | 1 cut(s) | 1229 |
| NdeI | CATATG | 1 cut(s) | 668 |
| NdeII | GATC | 7 cut(s) | 235, 485, 774, 1018, 1176, 1207, 1269 |
| NlaIV | GGNNCC | 2 cut(s) | 138, 990 |
| NmuCI | GTSAC | 2 cut(s) | 371, 800 |
| NsiI | ATGCAT | 2 cut(s) | 728, 1258 |
| NspI | RCATGY | 3 cut(s) | 921, 1205, 1473 |
| PaeI | GCATGC | 1 cut(s) | 1473 |
| PaeR7I | CTCGAG | 1 cut(s) | 81 |
| PciI | ACATGT | 1 cut(s) | 917 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 55 |
| PctI | GAATGC | 1 cut(s) | 759 |
| PfeI | GAWTC | 7 cut(s) | 52, 188, 350, 459, 941, 1222, 1306 |
| PkrI | GCNGC | 3 cut(s) | 712, 1372, 1461 |
| PpuMI | RGGWCCY | 1 cut(s) | 136 |
| PscI | ACATGT | 1 cut(s) | 917 |
| PshAI | GACNNNNGTC | 1 cut(s) | 470 |
| Psp124BI | GAGCTC | 1 cut(s) | 66 |
| Psp5II | RGGWCCY | 1 cut(s) | 136 |
| Psp6I | CCWGG | 1 cut(s) | 359 |
| PspEI | GGTNACC | 1 cut(s) | 800 |
| PspGI | CCWGG | 1 cut(s) | 359 |
| PspN4I | GGNNCC | 2 cut(s) | 138, 990 |
| PspPI | GGNCC | 3 cut(s) | 136, 181, 692 |
| PspPPI | RGGWCCY | 1 cut(s) | 136 |
| PspXI | VCTCGAGB | 1 cut(s) | 81 |
| PstNI | CAGNNNCTG | 2 cut(s) | 863, 1370 |
| PsuI | RGATCY | 2 cut(s) | 235, 485 |
| RsaI | GTAC | 5 cut(s) | 87, 281, 922, 990, 1056 |
| RsaNI | GTAC | 5 cut(s) | 86, 280, 921, 989, 1055 |
| SacI | GAGCTC | 1 cut(s) | 66 |
| SaqAI | TTAA | 7 cut(s) | 120, 404, 746, 789, 1188, 1448, 1480 |
| SatI | GCNGC | 3 cut(s) | 711, 1371, 1460 |
| Sau3AI | GATC | 7 cut(s) | 235, 485, 774, 1018, 1176, 1207, 1269 |
| Sau96I | GGNCC | 3 cut(s) | 136, 181, 692 |
| ScaI | AGTACT | 1 cut(s) | 87 |
| ScrFI | CCNGG | 2 cut(s) | 361, 1229 |
| SduI | GDGCHC | 1 cut(s) | 66 |
| SfaNI | GCATC | 7 cut(s) | 713, 817, 880, 954, 956, 1036, 1243 |
| Sfr274I | CTCGAG | 1 cut(s) | 81 |
| SinI | GGWCC | 2 cut(s) | 136, 181 |
| SlaI | CTCGAG | 1 cut(s) | 81 |
| SmlI | CTYRAG | 3 cut(s) | 81, 902, 1447 |
| SmoI | CTYRAG | 3 cut(s) | 81, 902, 1447 |
| SpeI | ACTAGT | 1 cut(s) | 143 |
| SphI | GCATGC | 1 cut(s) | 1473 |
| SsiI | CCGC | 1 cut(s) | 711 |
| SspI | AATATT | 2 cut(s) | 1081, 1401 |
| SspMI | CTAG | 4 cut(s) | 144, 275, 1032, 1250 |
| SstI | GAGCTC | 1 cut(s) | 66 |
| StyD4I | CCNGG | 2 cut(s) | 359, 1227 |
| TaaI | ACNGT | 4 cut(s) | 158, 869, 973, 1121 |
| TaiI | ACGT | 2 cut(s) | 27, 378 |
| TaqI | TCGA | 4 cut(s) | 82, 652, 975, 1206 |
| TatI | WGTACW | 1 cut(s) | 85 |
| TauI | GCSGC | 1 cut(s) | 713 |
| TfiI | GAWTC | 7 cut(s) | 52, 188, 350, 459, 941, 1222, 1306 |
| Tru1I | TTAA | 7 cut(s) | 120, 404, 746, 789, 1188, 1448, 1480 |
| Tru9I | TTAA | 7 cut(s) | 120, 404, 746, 789, 1188, 1448, 1480 |
| TscAI | CASTG | 5 cut(s) | 229, 864, 874, 1316, 1365 |
| TseFI | GTSAC | 2 cut(s) | 371, 800 |
| TseI | GCWGC | 2 cut(s) | 1370, 1459 |
| Tsp45I | GTSAC | 2 cut(s) | 371, 800 |
| TspDTI | ATGAA | 4 cut(s) | 513, 639, 1235, 1312 |
| TspGWI | ACGGA | 1 cut(s) | 146 |
| TspRI | CASTG | 5 cut(s) | 229, 864, 874, 1316, 1365 |
| Vha464I | CTTAAG | 1 cut(s) | 1447 |
| VpaK11BI | GGWCC | 2 cut(s) | 136, 181 |
| XapI | RAATTY | 6 cut(s) | 17, 500, 607, 836, 883, 1244 |
| XbaI | TCTAGA | 1 cut(s) | 1249 |
| XceI | RCATGY | 3 cut(s) | 921, 1205, 1473 |
| XhoI | CTCGAG | 1 cut(s) | 81 |
| XspI | CTAG | 4 cut(s) | 144, 275, 1032, 1250 |
| ZrmI | AGTACT | 1 cut(s) | 87 |
| Zsp2I | ATGCAT | 2 cut(s) | 728, 1258 |