Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G56120 |
| fragaria_vesca | FvH4_1g05380 |
| malus_domestica | MD02G1062400.v1.1 |
| pyrus_communis | pycom02g04360 |
| rosa_chinensis | RchiOBHm_Chr2g0091071 |
| rosa_laevigata | RLG00000016174 |
| rosa_multiflora | Rmu_sc0005171.1_g000009 |
| rosa_roxburghii | Rroxscaffold_2G00150620 |
| rosa_rugosa | Rorug02G0012300 |
| rosa_samantha | Rh2AG058400 Rh2BG056800 Rh2CG059000 Rh2DG058300 |
| rosa_wichuraiana | Rw2G005080 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 287, 1006 |
| Acc65I | GGTACC | 1 cut(s) | 913 |
| AccB1I | GGYRCC | 1 cut(s) | 913 |
| AccI | GTMKAC | 1 cut(s) | 946 |
| AccII | CGCG | 1 cut(s) | 435 |
| AccIII | TCCGGA | 3 cut(s) | 208, 679, 1197 |
| AciI | CCGC | 2 cut(s) | 115, 639 |
| AclI | AACGTT | 1 cut(s) | 1044 |
| AclWI | GGATC | 2 cut(s) | 421, 1189 |
| AcsI | RAATTY | 5 cut(s) | 17, 216, 535, 764, 811 |
| AcuI | CTGAAG | 3 cut(s) | 251, 719, 1305 |
| AfaI | GTAC | 3 cut(s) | 281, 850, 915 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 797, 878 |
| AflII | CTTAAG | 1 cut(s) | 1372 |
| AflIII | ACRYGT | 2 cut(s) | 143, 845 |
| AgsI | TTSAA | 9 cut(s) | 34, 251, 449, 508, 540, 811, 978, 1169, 1303 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 527, 559 |
| AluBI | AGCT | 6 cut(s) | 64, 511, 522, 584, 1055, 1377 |
| AluI | AGCT | 6 cut(s) | 64, 511, 522, 584, 1055, 1377 |
| Alw26I | GTCTC | 1 cut(s) | 836 |
| AlwI | GGATC | 2 cut(s) | 421, 1189 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 791, 1295 |
| Ama87I | CYCGRG | 1 cut(s) | 275 |
| Aor13HI | TCCGGA | 3 cut(s) | 208, 679, 1197 |
| AoxI | GGCC | 2 cut(s) | 619, 1305 |
| ApeKI | GCWGC | 2 cut(s) | 1295, 1384 |
| ApoI | RAATTY | 5 cut(s) | 17, 216, 535, 764, 811 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 255, 1221 |
| Asp718I | GGTACC | 1 cut(s) | 913 |
| AspLEI | GCGC | 2 cut(s) | 46, 296 |
| AspS9I | GGNCC | 3 cut(s) | 136, 620, 1306 |
| AsuHPI | GGTGA | 2 cut(s) | 740, 1204 |
| AvaI | CYCGRG | 1 cut(s) | 275 |
| AvaII | GGWCC | 1 cut(s) | 136 |
| BanI | GGYRCC | 1 cut(s) | 913 |
| BauI | CACGAG | 1 cut(s) | 57 |
| BbvI | GCAGC | 2 cut(s) | 1282, 1371 |
| BccI | CCATC | 3 cut(s) | 318, 775, 1028 |
| BclI | TGATCA | 2 cut(s) | 1104, 1132 |
| BcoDI | GTCTC | 1 cut(s) | 836 |
| BfaI | CTAG | 2 cut(s) | 957, 1175 |
| BfmI | CTRYAG | 1 cut(s) | 999 |
| BfoI | RGCGCY | 1 cut(s) | 47 |
| BfrI | CTTAAG | 1 cut(s) | 1372 |
| BfuAI | ACCTGC | 2 cut(s) | 287, 1006 |
| BisI | GCNGC | 3 cut(s) | 639, 1296, 1385 |
| BlsI | GCNGC | 3 cut(s) | 640, 1297, 1386 |
| Bme18I | GGWCC | 1 cut(s) | 136 |
| BmeT110I | CYCGRG | 1 cut(s) | 275 |
| BmgT120I | GGNCC | 3 cut(s) | 136, 620, 1306 |
| BmiI | GGNNCC | 3 cut(s) | 138, 870, 915 |
| BmsI | GCATC | 5 cut(s) | 641, 745, 808, 961, 1168 |
| BoxI | GACNNNNGTC | 1 cut(s) | 398 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1204, 1236 |
| BpmI | CTGGAG | 1 cut(s) | 969 |
| BsaBI | GATNNNNATC | 1 cut(s) | 418 |
| BsaWI | WCCGGW | 3 cut(s) | 208, 679, 1197 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 797, 878 |
| Bse1I | ACTGG | 1 cut(s) | 986 |
| Bse3DI | GCAATG | 3 cut(s) | 889, 1234, 1368 |
| Bse8I | GATNNNNATC | 1 cut(s) | 418 |
| BseAI | TCCGGA | 3 cut(s) | 208, 679, 1197 |
| BseGI | GGATG | 5 cut(s) | 13, 352, 656, 1248, 1348 |
| BseJI | GATNNNNATC | 1 cut(s) | 418 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 797, 878 |
| BseMI | GCAATG | 3 cut(s) | 889, 1234, 1368 |
| BseMII | CTCAG | 2 cut(s) | 587, 1089 |
| BseNI | ACTGG | 1 cut(s) | 986 |
| BseXI | GCAGC | 2 cut(s) | 1282, 1371 |
| BseYI | CCCAGC | 1 cut(s) | 584 |
| Bsh1236I | CGCG | 1 cut(s) | 435 |
| BshFI | GGCC | 2 cut(s) | 621, 1307 |
| BshNI | GGYRCC | 1 cut(s) | 913 |
| BsiHKCI | CYCGRG | 1 cut(s) | 275 |
| BsiSI | CCGG | 4 cut(s) | 209, 680, 1154, 1198 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 797, 878 |
| BsmAI | GTCTC | 1 cut(s) | 836 |
| BsmI | GAATGC | 2 cut(s) | 687, 894 |
| BsnI | GGCC | 2 cut(s) | 621, 1307 |
| BsoBI | CYCGRG | 1 cut(s) | 275 |
| Bsp13I | TCCGGA | 3 cut(s) | 208, 679, 1197 |
| Bsp143I | GATC | 5 cut(s) | 413, 702, 1104, 1132, 1194 |
| BspACI | CCGC | 2 cut(s) | 115, 639 |
| BspANI | GGCC | 2 cut(s) | 621, 1307 |
| BspCNI | CTCAG | 2 cut(s) | 586, 1090 |
| BspEI | TCCGGA | 3 cut(s) | 208, 679, 1197 |
| BspFNI | CGCG | 1 cut(s) | 435 |
| BspLI | GGNNCC | 3 cut(s) | 138, 870, 915 |
| BspMAI | CTGCAG | 1 cut(s) | 1003 |
| BspMI | ACCTGC | 2 cut(s) | 287, 1006 |
| BspPI | GGATC | 2 cut(s) | 421, 1189 |
| BspT107I | GGYRCC | 1 cut(s) | 913 |
| BspTI | CTTAAG | 1 cut(s) | 1372 |
| BsrDI | GCAATG | 3 cut(s) | 889, 1234, 1368 |
| BsrI | ACTGG | 1 cut(s) | 986 |
| BssMI | GATC | 5 cut(s) | 413, 702, 1104, 1132, 1194 |
| BssSI | CACGAG | 1 cut(s) | 57 |
| Bst2BI | CACGAG | 1 cut(s) | 57 |
| Bst4CI | ACNGT | 4 cut(s) | 158, 898, 944, 1030 |
| Bst6I | CTCTTC | 1 cut(s) | 1227 |
| BstAFI | CTTAAG | 1 cut(s) | 1372 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1295 |
| BstC8I | GCNNGC | 2 cut(s) | 437, 1396 |
| BstDEI | CTNAG | 2 cut(s) | 573, 1098 |
| BstEII | GGTNACC | 1 cut(s) | 728 |
| BstF5I | GGATG | 5 cut(s) | 13, 352, 656, 1248, 1348 |
| BstFNI | CGCG | 1 cut(s) | 435 |
| BstH2I | RGCGCY | 1 cut(s) | 47 |
| BstHHI | GCGC | 2 cut(s) | 46, 296 |
| BstKTI | GATC | 5 cut(s) | 416, 705, 1107, 1135, 1197 |
| BstMAI | GTCTC | 1 cut(s) | 836 |
| BstMBI | GATC | 5 cut(s) | 413, 702, 1104, 1132, 1194 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 635, 1071, 1295, 1304 |
| BstNSI | RCATGY | 3 cut(s) | 147, 849, 1130 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 398 |
| BstPI | GGTNACC | 1 cut(s) | 728 |
| BstSFI | CTRYAG | 1 cut(s) | 999 |
| BstUI | CGCG | 1 cut(s) | 435 |
| BstV1I | GCAGC | 2 cut(s) | 1282, 1371 |
| BstX2I | RGATCY | 1 cut(s) | 413 |
| BstYI | RGATCY | 1 cut(s) | 413 |
| BsuRI | GGCC | 2 cut(s) | 621, 1307 |
| BtsCI | GGATG | 5 cut(s) | 13, 352, 656, 1248, 1348 |
| BtsI | GCAGTG | 1 cut(s) | 792 |
| BtsIMutI | CAGTG | 1 cut(s) | 792 |
| BveI | ACCTGC | 2 cut(s) | 287, 1006 |
| Cac8I | GCNNGC | 2 cut(s) | 437, 1396 |
| CaiI | CAGNNNCTG | 2 cut(s) | 791, 1295 |
| CfoI | GCGC | 2 cut(s) | 46, 296 |
| Cfr13I | GGNCC | 3 cut(s) | 136, 620, 1306 |
| Csp6I | GTAC | 3 cut(s) | 280, 849, 914 |
| CviAII | CATG | 9 cut(s) | 144, 163, 323, 551, 734, 826, 846, 1127, 1136 |
| CviQI | GTAC | 3 cut(s) | 280, 849, 914 |
| DdeI | CTNAG | 2 cut(s) | 573, 1098 |
| DpnI | GATC | 5 cut(s) | 415, 704, 1106, 1134, 1196 |
| DpnII | GATC | 5 cut(s) | 413, 702, 1104, 1132, 1194 |
| Eam1104I | CTCTTC | 1 cut(s) | 1227 |
| EarI | CTCTTC | 1 cut(s) | 1227 |
| Eco47I | GGWCC | 1 cut(s) | 136 |
| Eco57I | CTGAAG | 3 cut(s) | 251, 719, 1305 |
| Eco88I | CYCGRG | 1 cut(s) | 275 |
| Eco91I | GGTNACC | 1 cut(s) | 728 |
| EcoO109I | RGGNCCY | 1 cut(s) | 136 |
| EcoO65I | GGTNACC | 1 cut(s) | 728 |
| EcoRI | GAATTC | 3 cut(s) | 216, 535, 811 |
| EcoT22I | ATGCAT | 2 cut(s) | 656, 1183 |
| FaeI | CATG | 9 cut(s) | 147, 166, 326, 554, 737, 829, 849, 1130, 1139 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 1383 |
| FatI | CATG | 9 cut(s) | 143, 162, 322, 550, 733, 825, 845, 1126, 1135 |
| FauI | CCCGC | 1 cut(s) | 122 |
| FauNDI | CATATG | 1 cut(s) | 596 |
| FbaI | TGATCA | 2 cut(s) | 1104, 1132 |
| FblI | GTMKAC | 1 cut(s) | 946 |
| Fnu4HI | GCNGC | 3 cut(s) | 639, 1296, 1385 |
| FokI | GGATG | 5 cut(s) | 20, 359, 663, 1235, 1355 |
| Fsp4HI | GCNGC | 3 cut(s) | 639, 1296, 1385 |
| FspBI | CTAG | 2 cut(s) | 957, 1175 |
| GlaI | GCGC | 2 cut(s) | 45, 295 |
| GluI | GCNGC | 3 cut(s) | 639, 1296, 1385 |
| GsaI | CCCAGC | 1 cut(s) | 588 |
| GsuI | CTGGAG | 1 cut(s) | 969 |
| HaeII | RGCGCY | 1 cut(s) | 47 |
| HaeIII | GGCC | 2 cut(s) | 621, 1307 |
| HapII | CCGG | 4 cut(s) | 209, 680, 1154, 1198 |
| HhaI | GCGC | 2 cut(s) | 46, 296 |
| Hin1II | CATG | 9 cut(s) | 147, 166, 326, 554, 737, 829, 849, 1130, 1139 |
| Hin6I | GCGC | 2 cut(s) | 44, 294 |
| HinP1I | GCGC | 2 cut(s) | 44, 294 |
| HincII | GTYRAC | 1 cut(s) | 270 |
| HindII | GTYRAC | 1 cut(s) | 270 |
| HindIII | AAGCTT | 2 cut(s) | 509, 1375 |
| HinfI | GANTC | 5 cut(s) | 52, 188, 387, 875, 1231 |
| HpaII | CCGG | 4 cut(s) | 209, 680, 1154, 1198 |
| HphI | GGTGA | 2 cut(s) | 740, 1204 |
| Hpy166II | GTNNAC | 4 cut(s) | 270, 739, 940, 947 |
| Hpy8I | GTNNAC | 4 cut(s) | 270, 739, 940, 947 |
| HpyAV | CCTTC | 3 cut(s) | 226, 1160, 1297 |
| HpyCH4III | ACNGT | 4 cut(s) | 158, 898, 944, 1030 |
| HpyCH4IV | ACGT | 3 cut(s) | 303, 936, 1044 |
| HpyCH4V | TGCA | 9 cut(s) | 28, 69, 184, 243, 654, 665, 710, 1001, 1181 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 635, 1071, 1295, 1304 |
| HpyF3I | CTNAG | 2 cut(s) | 573, 1098 |
| HpySE526I | ACGT | 3 cut(s) | 303, 936, 1044 |
| Hsp92II | CATG | 9 cut(s) | 147, 166, 326, 554, 737, 829, 849, 1130, 1139 |
| HspAI | GCGC | 2 cut(s) | 44, 294 |
| Kpn2I | TCCGGA | 3 cut(s) | 208, 679, 1197 |
| KpnI | GGTACC | 1 cut(s) | 917 |
| Ksp22I | TGATCA | 2 cut(s) | 1104, 1132 |
| Kzo9I | GATC | 5 cut(s) | 413, 702, 1104, 1132, 1194 |
| LmnI | GCTCC | 4 cut(s) | 497, 1062, 1123, 1354 |
| Lsp1109I | GCAGC | 2 cut(s) | 1282, 1371 |
| LweI | GCATC | 5 cut(s) | 641, 745, 808, 961, 1168 |
| MaeI | CTAG | 2 cut(s) | 957, 1175 |
| MaeII | ACGT | 3 cut(s) | 303, 936, 1044 |
| MaeIII | GTNAC | 5 cut(s) | 158, 299, 475, 728, 925 |
| MalI | GATC | 5 cut(s) | 415, 704, 1106, 1134, 1196 |
| MboI | GATC | 5 cut(s) | 413, 702, 1104, 1132, 1194 |
| MboII | GAAGA | 2 cut(s) | 712, 1214 |
| MfeI | CAATTG | 1 cut(s) | 396 |
| MflI | RGATCY | 1 cut(s) | 413 |
| MlyI | GAGTC | 1 cut(s) | 884 |
| MmeI | TCCRAC | 2 cut(s) | 216, 391 |
| Mph1103I | ATGCAT | 2 cut(s) | 656, 1183 |
| MroI | TCCGGA | 3 cut(s) | 208, 679, 1197 |
| MroXI | GAANNNNTTC | 2 cut(s) | 255, 1221 |
| MseI | TTAA | 8 cut(s) | 120, 332, 674, 717, 990, 1373, 1401, 1405 |
| MspCI | CTTAAG | 1 cut(s) | 1372 |
| MspI | CCGG | 4 cut(s) | 209, 680, 1154, 1198 |
| MunI | CAATTG | 1 cut(s) | 396 |
| Mva1269I | GAATGC | 2 cut(s) | 687, 894 |
| MvnI | CGCG | 1 cut(s) | 435 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 635, 1071, 1295, 1304 |
| NdeI | CATATG | 1 cut(s) | 596 |
| NdeII | GATC | 5 cut(s) | 413, 702, 1104, 1132, 1194 |
| NlaIII | CATG | 9 cut(s) | 147, 166, 326, 554, 737, 829, 849, 1130, 1139 |
| NlaIV | GGNNCC | 3 cut(s) | 138, 870, 915 |
| NmuCI | GTSAC | 2 cut(s) | 299, 728 |
| NsiI | ATGCAT | 2 cut(s) | 656, 1183 |
| NspI | RCATGY | 3 cut(s) | 147, 849, 1130 |
| PacI | TTAATTAA | 1 cut(s) | 1405 |
| PciI | ACATGT | 2 cut(s) | 143, 845 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 55 |
| PctI | GAATGC | 2 cut(s) | 687, 894 |
| PdmI | GAANNNNTTC | 2 cut(s) | 255, 1221 |
| PfeI | GAWTC | 4 cut(s) | 52, 188, 387, 1231 |
| PkrI | GCNGC | 3 cut(s) | 640, 1297, 1386 |
| PleI | GAGTC | 1 cut(s) | 883 |
| PpsI | GAGTC | 1 cut(s) | 883 |
| PpuMI | RGGWCCY | 1 cut(s) | 136 |
| PscI | ACATGT | 2 cut(s) | 143, 845 |
| PshAI | GACNNNNGTC | 1 cut(s) | 398 |
| Psp1406I | AACGTT | 1 cut(s) | 1044 |
| Psp5II | RGGWCCY | 1 cut(s) | 136 |
| PspEI | GGTNACC | 1 cut(s) | 728 |
| PspFI | CCCAGC | 1 cut(s) | 584 |
| PspN4I | GGNNCC | 3 cut(s) | 138, 870, 915 |
| PspPI | GGNCC | 3 cut(s) | 136, 620, 1306 |
| PspPPI | RGGWCCY | 1 cut(s) | 136 |
| PstI | CTGCAG | 1 cut(s) | 1003 |
| PstNI | CAGNNNCTG | 2 cut(s) | 791, 1295 |
| PsuI | RGATCY | 1 cut(s) | 413 |
| RsaI | GTAC | 3 cut(s) | 281, 850, 915 |
| RsaNI | GTAC | 3 cut(s) | 280, 849, 914 |
| SaqAI | TTAA | 8 cut(s) | 120, 332, 674, 717, 990, 1373, 1401, 1405 |
| SatI | GCNGC | 3 cut(s) | 639, 1296, 1385 |
| Sau3AI | GATC | 5 cut(s) | 413, 702, 1104, 1132, 1194 |
| Sau96I | GGNCC | 3 cut(s) | 136, 620, 1306 |
| SchI | GAGTC | 1 cut(s) | 884 |
| SfaNI | GCATC | 5 cut(s) | 641, 745, 808, 961, 1168 |
| SfcI | CTRYAG | 1 cut(s) | 999 |
| SinI | GGWCC | 1 cut(s) | 136 |
| SmlI | CTYRAG | 1 cut(s) | 1372 |
| SmoI | CTYRAG | 1 cut(s) | 1372 |
| SsiI | CCGC | 2 cut(s) | 115, 639 |
| SspI | AATATT | 2 cut(s) | 1208, 1326 |
| SspMI | CTAG | 2 cut(s) | 957, 1175 |
| TaaI | ACNGT | 4 cut(s) | 158, 898, 944, 1030 |
| TaiI | ACGT | 3 cut(s) | 306, 939, 1047 |
| TaqI | TCGA | 3 cut(s) | 82, 580, 753 |
| TauI | GCSGC | 1 cut(s) | 641 |
| TfiI | GAWTC | 4 cut(s) | 52, 188, 387, 1231 |
| Tru1I | TTAA | 8 cut(s) | 120, 332, 674, 717, 990, 1373, 1401, 1405 |
| Tru9I | TTAA | 8 cut(s) | 120, 332, 674, 717, 990, 1373, 1401, 1405 |
| TscAI | CASTG | 1 cut(s) | 792 |
| TseFI | GTSAC | 2 cut(s) | 299, 728 |
| TseI | GCWGC | 2 cut(s) | 1295, 1384 |
| Tsp45I | GTSAC | 2 cut(s) | 299, 728 |
| TspDTI | ATGAA | 7 cut(s) | 240, 567, 1038, 1160, 1223, 1237, 1319 |
| TspGWI | ACGGA | 1 cut(s) | 146 |
| TspRI | CASTG | 1 cut(s) | 792 |
| Vha464I | CTTAAG | 1 cut(s) | 1372 |
| VpaK11BI | GGWCC | 1 cut(s) | 136 |
| XapI | RAATTY | 5 cut(s) | 17, 216, 535, 764, 811 |
| XbaI | TCTAGA | 1 cut(s) | 1174 |
| XceI | RCATGY | 3 cut(s) | 147, 849, 1130 |
| XmiI | GTMKAC | 1 cut(s) | 946 |
| XmnI | GAANNNNTTC | 2 cut(s) | 255, 1221 |
| XspI | CTAG | 2 cut(s) | 957, 1175 |
| Zsp2I | ATGCAT | 2 cut(s) | 656, 1183 |