Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G56120 |
| fragaria_vesca | FvH4_1g05380 |
| malus_domestica | MD02G1062400.v1.1 |
| pyrus_communis | pycom02g04360 |
| rosa_chinensis | RchiOBHm_Chr2g0091071 |
| rosa_laevigata | RLG00000016174 |
| rosa_multiflora | Rmu_sc0005171.1_g000009 |
| rosa_roxburghii | Rroxscaffold_2G00150620 |
| rosa_rugosa | Rorug02G0012300 |
| rosa_samantha | Rh2AG058400 Rh2BG056800 Rh2CG059000 Rh2DG058300 |
| rosa_wichuraiana | Rw2G005080 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 827 |
| Acc65I | GGTACC | 1 cut(s) | 284 |
| AccB1I | GGYRCC | 1 cut(s) | 284 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 547 |
| AccBSI | CCGCTC | 2 cut(s) | 271, 652 |
| AccI | GTMKAC | 1 cut(s) | 320 |
| AciI | CCGC | 3 cut(s) | 269, 652, 774 |
| AclI | AACGTT | 1 cut(s) | 13 |
| AclWI | GGATC | 2 cut(s) | 260, 457 |
| AcuI | CTGAAG | 1 cut(s) | 145 |
| AfaI | GTAC | 3 cut(s) | 221, 286, 682 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 547, 787 |
| AgsI | TTSAA | 2 cut(s) | 77, 472 |
| AjnI | CCWGG | 1 cut(s) | 314 |
| AleI | CACNNNNGTG | 2 cut(s) | 32, 43 |
| AluBI | AGCT | 2 cut(s) | 690, 725 |
| AluI | AGCT | 2 cut(s) | 690, 725 |
| Alw26I | GTCTC | 4 cut(s) | 53, 65, 606, 731 |
| AlwI | GGATC | 2 cut(s) | 260, 457 |
| Ama87I | CYCGRG | 2 cut(s) | 445, 703 |
| Asp718I | GGTACC | 1 cut(s) | 284 |
| AsuC2I | CCSGG | 2 cut(s) | 704, 705 |
| AvaI | CYCGRG | 2 cut(s) | 445, 703 |
| BanI | GGYRCC | 1 cut(s) | 284 |
| BanII | GRGCYC | 1 cut(s) | 710 |
| BbsI | GAAGAC | 1 cut(s) | 810 |
| BccI | CCATC | 2 cut(s) | 192, 193 |
| BciT130I | CCWGG | 1 cut(s) | 316 |
| BciVI | GTATCC | 1 cut(s) | 406 |
| BcnI | CCSGG | 2 cut(s) | 704, 705 |
| BcoDI | GTCTC | 4 cut(s) | 53, 65, 606, 731 |
| BfaI | CTAG | 3 cut(s) | 500, 663, 722 |
| BfmI | CTRYAG | 1 cut(s) | 327 |
| BfuAI | ACCTGC | 1 cut(s) | 827 |
| BfuI | GTATCC | 1 cut(s) | 406 |
| BglII | AGATCT | 1 cut(s) | 130 |
| Bme1390I | CCNGG | 3 cut(s) | 316, 704, 705 |
| BmeT110I | CYCGRG | 2 cut(s) | 445, 703 |
| BmiI | GGNNCC | 4 cut(s) | 24, 286, 312, 701 |
| BmrFI | CCNGG | 3 cut(s) | 316, 704, 705 |
| BmrI | ACTGGG | 2 cut(s) | 89, 426 |
| BmsI | GCATC | 1 cut(s) | 794 |
| BmuI | ACTGGG | 2 cut(s) | 89, 426 |
| BpiI | GAAGAC | 1 cut(s) | 810 |
| BpuEI | CTTGAG | 1 cut(s) | 155 |
| BpuMI | CCSGG | 2 cut(s) | 704, 705 |
| BsaI | GGTCTC | 1 cut(s) | 65 |
| BsaJI | CCNNGG | 2 cut(s) | 314, 703 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 547, 787 |
| Bse1I | ACTGG | 4 cut(s) | 84, 421, 510, 524 |
| BseBI | CCWGG | 1 cut(s) | 316 |
| BseDI | CCNNGG | 2 cut(s) | 314, 703 |
| BseGI | GGATG | 2 cut(s) | 204, 809 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 547, 787 |
| BseMII | CTCAG | 2 cut(s) | 104, 677 |
| BseNI | ACTGG | 4 cut(s) | 84, 421, 510, 524 |
| BseRI | GAGGAG | 1 cut(s) | 168 |
| BshNI | GGYRCC | 1 cut(s) | 284 |
| BsiHKCI | CYCGRG | 2 cut(s) | 445, 703 |
| BsiSI | CCGG | 1 cut(s) | 704 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 547, 787 |
| BsmAI | GTCTC | 4 cut(s) | 53, 65, 606, 731 |
| BsmI | GAATGC | 1 cut(s) | 640 |
| Bso31I | GGTCTC | 1 cut(s) | 65 |
| BsoBI | CYCGRG | 2 cut(s) | 445, 703 |
| Bsp1286I | GDGCHC | 1 cut(s) | 710 |
| Bsp143I | GATC | 4 cut(s) | 130, 250, 265, 449 |
| BspACI | CCGC | 3 cut(s) | 269, 652, 774 |
| BspCNI | CTCAG | 2 cut(s) | 103, 678 |
| BspLI | GGNNCC | 4 cut(s) | 24, 286, 312, 701 |
| BspMI | ACCTGC | 1 cut(s) | 827 |
| BspPI | GGATC | 2 cut(s) | 260, 457 |
| BspT107I | GGYRCC | 1 cut(s) | 284 |
| BspTNI | GGTCTC | 1 cut(s) | 65 |
| BsrBI | CCGCTC | 2 cut(s) | 271, 652 |
| BsrI | ACTGG | 4 cut(s) | 84, 421, 510, 524 |
| BssECI | CCNNGG | 2 cut(s) | 314, 703 |
| BssMI | GATC | 4 cut(s) | 130, 250, 265, 449 |
| BssNAI | GTATAC | 1 cut(s) | 321 |
| Bst1107I | GTATAC | 1 cut(s) | 321 |
| Bst2UI | CCWGG | 1 cut(s) | 316 |
| Bst4CI | ACNGT | 1 cut(s) | 331 |
| Bst6I | CTCTTC | 1 cut(s) | 564 |
| BstDEI | CTNAG | 3 cut(s) | 90, 394, 686 |
| BstEII | GGTNACC | 1 cut(s) | 839 |
| BstF5I | GGATG | 2 cut(s) | 204, 809 |
| BstKTI | GATC | 4 cut(s) | 133, 253, 268, 452 |
| BstMAI | GTCTC | 4 cut(s) | 53, 65, 606, 731 |
| BstMBI | GATC | 4 cut(s) | 130, 250, 265, 449 |
| BstNI | CCWGG | 1 cut(s) | 316 |
| BstPI | GGTNACC | 1 cut(s) | 839 |
| BstSCI | CCNGG | 3 cut(s) | 314, 702, 703 |
| BstSFI | CTRYAG | 1 cut(s) | 327 |
| BstV2I | GAAGAC | 1 cut(s) | 810 |
| BstX2I | RGATCY | 1 cut(s) | 130 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 34 |
| BstYI | RGATCY | 1 cut(s) | 130 |
| BstZ17I | GTATAC | 1 cut(s) | 321 |
| BsuI | GTATCC | 1 cut(s) | 406 |
| BtgZI | GCGATG | 1 cut(s) | 681 |
| BtsCI | GGATG | 2 cut(s) | 204, 809 |
| BtsIMutI | CAGTG | 1 cut(s) | 253 |
| BveI | ACCTGC | 1 cut(s) | 827 |
| Cfr9I | CCCGGG | 1 cut(s) | 703 |
| CseI | GACGC | 1 cut(s) | 136 |
| Csp6I | GTAC | 3 cut(s) | 220, 285, 681 |
| CviAII | CATG | 1 cut(s) | 274 |
| CviJI | RGCY | 7 cut(s) | 88, 159, 487, 551, 690, 708, 725 |
| CviKI_1 | RGCY | 7 cut(s) | 88, 159, 487, 551, 690, 708, 725 |
| CviQI | GTAC | 3 cut(s) | 220, 285, 681 |
| DdeI | CTNAG | 3 cut(s) | 90, 394, 686 |
| DpnI | GATC | 4 cut(s) | 132, 252, 267, 451 |
| DpnII | GATC | 4 cut(s) | 130, 250, 265, 449 |
| Eam1104I | CTCTTC | 1 cut(s) | 564 |
| EarI | CTCTTC | 1 cut(s) | 564 |
| Eco24I | GRGCYC | 1 cut(s) | 710 |
| Eco31I | GGTCTC | 1 cut(s) | 65 |
| Eco57I | CTGAAG | 1 cut(s) | 145 |
| Eco88I | CYCGRG | 2 cut(s) | 445, 703 |
| Eco91I | GGTNACC | 1 cut(s) | 839 |
| EcoO65I | GGTNACC | 1 cut(s) | 839 |
| EcoRII | CCWGG | 1 cut(s) | 314 |
| EcoT22I | ATGCAT | 2 cut(s) | 640, 809 |
| EcoT38I | GRGCYC | 1 cut(s) | 710 |
| FaeI | CATG | 1 cut(s) | 277 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 364, 396, 578, 610 |
| FatI | CATG | 1 cut(s) | 273 |
| FauI | CCCGC | 1 cut(s) | 645 |
| FblI | GTMKAC | 1 cut(s) | 320 |
| FokI | GGATG | 2 cut(s) | 211, 816 |
| FriOI | GRGCYC | 1 cut(s) | 710 |
| FspBI | CTAG | 3 cut(s) | 500, 663, 722 |
| HapII | CCGG | 1 cut(s) | 704 |
| HgaI | GACGC | 1 cut(s) | 136 |
| Hin1II | CATG | 1 cut(s) | 277 |
| HincII | GTYRAC | 1 cut(s) | 795 |
| HindII | GTYRAC | 1 cut(s) | 795 |
| HinfI | GANTC | 3 cut(s) | 468, 537, 599 |
| HpaI | GTTAAC | 1 cut(s) | 795 |
| HpaII | CCGG | 1 cut(s) | 704 |
| Hpy166II | GTNNAC | 4 cut(s) | 103, 241, 321, 795 |
| Hpy188I | TCNGA | 4 cut(s) | 93, 152, 164, 687 |
| Hpy188III | TCNNGA | 2 cut(s) | 361, 447 |
| Hpy8I | GTNNAC | 4 cut(s) | 103, 241, 321, 795 |
| HpyAV | CCTTC | 2 cut(s) | 199, 427 |
| HpyCH4III | ACNGT | 1 cut(s) | 331 |
| HpyCH4IV | ACGT | 1 cut(s) | 13 |
| HpyCH4V | TGCA | 8 cut(s) | 277, 292, 525, 638, 660, 719, 807, 836 |
| HpyF3I | CTNAG | 3 cut(s) | 90, 394, 686 |
| HpySE526I | ACGT | 1 cut(s) | 13 |
| Hsp92II | CATG | 1 cut(s) | 277 |
| KpnI | GGTACC | 1 cut(s) | 288 |
| KspAI | GTTAAC | 1 cut(s) | 795 |
| Kzo9I | GATC | 4 cut(s) | 130, 250, 265, 449 |
| LweI | GCATC | 1 cut(s) | 794 |
| MaeI | CTAG | 3 cut(s) | 500, 663, 722 |
| MaeII | ACGT | 1 cut(s) | 13 |
| MaeIII | GTNAC | 4 cut(s) | 353, 477, 515, 839 |
| MalI | GATC | 4 cut(s) | 132, 252, 267, 451 |
| MbiI | CCGCTC | 2 cut(s) | 271, 652 |
| MboI | GATC | 4 cut(s) | 130, 250, 265, 449 |
| MboII | GAAGA | 6 cut(s) | 19, 126, 179, 572, 581, 810 |
| MflI | RGATCY | 1 cut(s) | 130 |
| MhlI | GDGCHC | 1 cut(s) | 710 |
| MlyI | GAGTC | 1 cut(s) | 608 |
| MmeI | TCCRAC | 2 cut(s) | 514, 697 |
| Mph1103I | ATGCAT | 2 cut(s) | 640, 809 |
| MseI | TTAA | 3 cut(s) | 305, 440, 794 |
| MslI | CAYNNNNRTG | 4 cut(s) | 32, 43, 407, 812 |
| MspI | CCGG | 1 cut(s) | 704 |
| MspR9I | CCNGG | 3 cut(s) | 316, 704, 705 |
| Mva1269I | GAATGC | 1 cut(s) | 640 |
| MvaI | CCWGG | 1 cut(s) | 316 |
| NciI | CCSGG | 2 cut(s) | 704, 705 |
| NdeII | GATC | 4 cut(s) | 130, 250, 265, 449 |
| NlaIII | CATG | 1 cut(s) | 277 |
| NlaIV | GGNNCC | 4 cut(s) | 24, 286, 312, 701 |
| NmuCI | GTSAC | 1 cut(s) | 515 |
| NsiI | ATGCAT | 2 cut(s) | 640, 809 |
| OliI | CACNNNNGTG | 2 cut(s) | 32, 43 |
| PctI | GAATGC | 1 cut(s) | 640 |
| PfeI | GAWTC | 2 cut(s) | 468, 537 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 547 |
| PleI | GAGTC | 1 cut(s) | 607 |
| PpsI | GAGTC | 1 cut(s) | 607 |
| Psp1406I | AACGTT | 1 cut(s) | 13 |
| Psp6I | CCWGG | 1 cut(s) | 314 |
| PspEI | GGTNACC | 1 cut(s) | 839 |
| PspGI | CCWGG | 1 cut(s) | 314 |
| PspN4I | GGNNCC | 4 cut(s) | 24, 286, 312, 701 |
| PsuI | RGATCY | 1 cut(s) | 130 |
| RsaI | GTAC | 3 cut(s) | 221, 286, 682 |
| RsaNI | GTAC | 3 cut(s) | 220, 285, 681 |
| RseI | CAYNNNNRTG | 4 cut(s) | 32, 43, 407, 812 |
| SaqAI | TTAA | 3 cut(s) | 305, 440, 794 |
| Sau3AI | GATC | 4 cut(s) | 130, 250, 265, 449 |
| SchI | GAGTC | 1 cut(s) | 608 |
| ScrFI | CCNGG | 3 cut(s) | 316, 704, 705 |
| SduI | GDGCHC | 1 cut(s) | 710 |
| SfaNI | GCATC | 1 cut(s) | 794 |
| SfcI | CTRYAG | 1 cut(s) | 327 |
| SmaI | CCCGGG | 1 cut(s) | 705 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 32, 43, 407, 812 |
| SmlI | CTYRAG | 1 cut(s) | 170 |
| SmoI | CTYRAG | 1 cut(s) | 170 |
| SsiI | CCGC | 3 cut(s) | 269, 652, 774 |
| SspI | AATATT | 1 cut(s) | 747 |
| SspMI | CTAG | 3 cut(s) | 500, 663, 722 |
| StyD4I | CCNGG | 3 cut(s) | 314, 702, 703 |
| TaaI | ACNGT | 1 cut(s) | 331 |
| TaiI | ACGT | 1 cut(s) | 16 |
| TatI | WGTACW | 1 cut(s) | 219 |
| TfiI | GAWTC | 2 cut(s) | 468, 537 |
| Tru1I | TTAA | 3 cut(s) | 305, 440, 794 |
| Tru9I | TTAA | 3 cut(s) | 305, 440, 794 |
| TscAI | CASTG | 1 cut(s) | 253 |
| TseFI | GTSAC | 1 cut(s) | 515 |
| Tsp45I | GTSAC | 1 cut(s) | 515 |
| TspDTI | ATGAA | 3 cut(s) | 130, 635, 642 |
| TspGWI | ACGGA | 2 cut(s) | 211, 816 |
| TspMI | CCCGGG | 1 cut(s) | 703 |
| TspRI | CASTG | 1 cut(s) | 253 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 547 |
| XmaI | CCCGGG | 1 cut(s) | 703 |
| XmiI | GTMKAC | 1 cut(s) | 320 |
| XspI | CTAG | 3 cut(s) | 500, 663, 722 |
| Zsp2I | ATGCAT | 2 cut(s) | 640, 809 |