Rroxscaffold_2G00150620

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016610
Physical Location & Seq
Reverse (-)
87989926 .. 87993720
3795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00150620.1

Sequence Viewer

Length: 1476 bp
ATGTTGGATGAGAGTAAATTTGATGTGCATTTGAAATTGTGGGCGCTTCGGATTCCTCGTGAGCTTTGCAAGGTCGCCACTCGAATACTAAATGGATATTTGCTTGATAAACCCCGCATTAAGCCGATTACGGAGGACCCAACATGTGAAAGAAACCGTTACATGATATTATCGGAGAGGGTGCAAGATTCCGACCTATCTATTATTCCGGATAGAATTCTCAATGAACTGAAGGATTTATGCAAGATTGAAGTAGTTCCATATTCGTTGACTCTCGGGTACTCATATTGGGGTGCAGACCATATTTTGAAGCAGATTCTGCCCCCGGAGTGGAGGTTCCTTCATCTTTTGAAACAATACAATAACCGTCACGTTGCCCATCTGAATATACATGATGAGTTAATTCCCTACAAGGATGTGATTGCCAAAGTTATTTATGATAAGAATTATCCAAGAATCAAGACAATTGTCAATAAAGTTGGATCTATTTCAAATGAGTTTCGCGTGCCGAAGTTTGAAGTTTTAGCAGGAGAAAAAGATATGGTTACAGAAGTGAAACAATATGGAGCGACTTTCAAGCTTGATTACAGCTTGGTCTATTGGAATTCAAGATTGGAACATGAACATATAAGGTTGGTTTCTCAGTTTCGAGCTGGGGAAGTCATATGTGATATGTTCGCTGGTATTGGCCCTTTTGCTATTCCTGCCGCACAGAAAGGATGCATCGTGTATGCAAATGACTTAAATCCGGATAGCATTCATTATCTGAAGATCAATGCAGAGATTAACAAGGTTGGTGACCATGTTCACGCATACAATCTTGATGCGAGAGAATTTGTTTCTCAGTTGATGACAGTGCCGGACTGTGGGAAAGAATTGAATTCAGATGCGTCCATGCTCAAATCTTGCGAGACATGTACCAATCAGGGTAGTCAGGAATCAGAATCAGGCAATGGAATGCTAAACATTGAGGCAAAAGAGGTACTAGATGGTGTTACTTCTAATGTGGACGGTCTACAAAGTTCTAGTAGGAATACAGATGCTTCAGTACCTCCAGTTAAAAGACCTGCAGATGTTTGTCAATCAGAGAATGGAAGTGTTAATGGCACAAACGTTCTTGTAGCTGGTAGGAGCAAAGGAAACAAGAATAAGAGAATAAGAGGCTCTGAGATCATCAATGTCAAGACTTGGGAGCATGTTGATCATGTGATAATGAACCTTCCGGCTTCTGCTCTTGAATTTCTAGATGCATTTAAAGGGGTGATCCAGAGAAAATATTGGAGGGAAACTCTTCCTTTGATTCACTGCTATTGCTTCATCCGAGCAAGTGAAACGCAAGAATATATTATTTCCGAGGCAGAGGCTGCTTTGAAGGCCCATATAAAAGACCCAATATTTCATAGGGTTAGGGATGTTGCTCCAAACAAGGCAATGTTTTGCTTAAGCTTTAGGCTGCCAGAAGCGTGCCTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Met_10

Protein Analysis

491

Amino Acids

55.78

Weight (kDa)

6.77

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW2_N_2 PF25133 124 - 179 9.9e-14 TYW2 N-terminal domain
TRM5-TYW2_MTfase PF02475 183 - 281 6.1e-25 TRM5/TYW2 methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016610)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56120
fragaria_vesca FvH4_1g05380
malus_domestica MD02G1062400.v1.1
pyrus_communis pycom02g04360
rosa_chinensis RchiOBHm_Chr2g0091071
rosa_laevigata RLG00000016174
rosa_multiflora Rmu_sc0005171.1_g000009
rosa_roxburghii Rroxscaffold_2G00150620
rosa_rugosa Rorug02G0012300
rosa_samantha Rh2AG058400 Rh2BG056800 Rh2CG059000 Rh2DG058300
rosa_wichuraiana Rw2G005080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1075
AccI GTMKAC 1 cut(s) 1015
AccII CGCG 1 cut(s) 504
AccIII TCCGGA 2 cut(s) 208, 748
AciI CCGC 2 cut(s) 115, 708
AclI AACGTT 1 cut(s) 1113
AclWI GGATC 2 cut(s) 490, 1258
AcsI RAATTY 6 cut(s) 17, 216, 604, 833, 880, 1238
AcuI CTGAAG 3 cut(s) 251, 788, 1029
AfaI GTAC 4 cut(s) 281, 919, 984, 1050
AfiI CCNNNNNNNGG 2 cut(s) 330, 866
AflII CTTAAG 1 cut(s) 1441
AflIII ACRYGT 2 cut(s) 143, 914
AjuI GAANNNNNNNTTGG 2 cut(s) 596, 628
AloI GAACNNNNNNTCC 2 cut(s) 320, 352
AluBI AGCT 6 cut(s) 64, 580, 591, 653, 1124, 1446
AluI AGCT 6 cut(s) 64, 580, 591, 653, 1124, 1446
Alw26I GTCTC 1 cut(s) 905
AlwI GGATC 2 cut(s) 490, 1258
AlwNI CAGNNNCTG 2 cut(s) 319, 1364
Ama87I CYCGRG 1 cut(s) 275
Aor13HI TCCGGA 2 cut(s) 208, 748
AoxI GGCC 2 cut(s) 688, 1374
ApeKI GCWGC 2 cut(s) 1364, 1453
ApoI RAATTY 6 cut(s) 17, 216, 604, 833, 880, 1238
Asp700I GAANNNNTTC 2 cut(s) 255, 1290
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 3 cut(s) 136, 689, 1375
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 809, 1273
AvaI CYCGRG 1 cut(s) 275
AvaII GGWCC 1 cut(s) 136
BauI CACGAG 1 cut(s) 57
BbvI GCAGC 2 cut(s) 1351, 1440
BccI CCATC 2 cut(s) 387, 983
BclI TGATCA 1 cut(s) 1201
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 1 cut(s) 905
BfaI CTAG 3 cut(s) 986, 1026, 1244
BfmI CTRYAG 1 cut(s) 1068
BfoI RGCGCY 1 cut(s) 47
BfrI CTTAAG 1 cut(s) 1441
BfuAI ACCTGC 1 cut(s) 1075
BisI GCNGC 3 cut(s) 708, 1365, 1454
BlsI GCNGC 3 cut(s) 709, 1366, 1455
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 136
BmeT110I CYCGRG 1 cut(s) 275
BmgT120I GGNCC 3 cut(s) 136, 689, 1375
BmiI GGNNCC 2 cut(s) 138, 338
BmrFI CCNGG 1 cut(s) 326
BmsI GCATC 6 cut(s) 710, 732, 814, 877, 1030, 1237
BoxI GACNNNNGTC 1 cut(s) 467
BplI GAGNNNNNCTC 2 cut(s) 1273, 1305
BpmI CTGGAG 1 cut(s) 1038
BpuMI CCSGG 1 cut(s) 326
BsaJI CCNNGG 2 cut(s) 324, 1353
BsaWI WCCGGW 2 cut(s) 208, 748
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 2 cut(s) 330, 866
Bse1I ACTGG 1 cut(s) 1055
Bse3DI GCAATG 2 cut(s) 958, 1437
BseAI TCCGGA 2 cut(s) 208, 748
BseDI CCNNGG 2 cut(s) 324, 1353
BseGI GGATG 5 cut(s) 13, 421, 725, 1317, 1417
BseLI CCNNNNNNNGG 2 cut(s) 330, 866
BseMI GCAATG 2 cut(s) 958, 1437
BseMII CTCAG 3 cut(s) 656, 857, 1158
BseNI ACTGG 1 cut(s) 1055
BseXI GCAGC 2 cut(s) 1351, 1440
BseYI CCCAGC 1 cut(s) 653
BsgI GTGCAG 1 cut(s) 315
Bsh1236I CGCG 1 cut(s) 504
BshFI GGCC 2 cut(s) 690, 1376
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 5 cut(s) 209, 326, 749, 860, 1223
BslI CCNNNNNNNGG 2 cut(s) 330, 866
BsmAI GTCTC 1 cut(s) 905
BsmI GAATGC 2 cut(s) 756, 963
BsnI GGCC 2 cut(s) 690, 1376
BsoBI CYCGRG 1 cut(s) 275
Bsp13I TCCGGA 2 cut(s) 208, 748
Bsp143I GATC 5 cut(s) 482, 771, 1170, 1201, 1263
BspACI CCGC 2 cut(s) 115, 708
BspANI GGCC 2 cut(s) 690, 1376
BspCNI CTCAG 3 cut(s) 655, 856, 1159
BspEI TCCGGA 2 cut(s) 208, 748
BspFNI CGCG 1 cut(s) 504
BspLI GGNNCC 2 cut(s) 138, 338
BspMAI CTGCAG 1 cut(s) 1072
BspMI ACCTGC 1 cut(s) 1075
BspPI GGATC 2 cut(s) 490, 1258
BspTI CTTAAG 1 cut(s) 1441
BsrDI GCAATG 2 cut(s) 958, 1437
BsrI ACTGG 1 cut(s) 1055
BssECI CCNNGG 2 cut(s) 324, 1353
BssMI GATC 5 cut(s) 482, 771, 1170, 1201, 1263
BssSI CACGAG 1 cut(s) 57
Bst2BI CACGAG 1 cut(s) 57
Bst4CI ACNGT 5 cut(s) 158, 368, 856, 866, 1013
Bst6I CTCTTC 1 cut(s) 1296
BstAFI CTTAAG 1 cut(s) 1441
BstAPI GCANNNNNTGC 2 cut(s) 319, 1364
BstC8I GCNNGC 2 cut(s) 506, 1465
BstDEI CTNAG 3 cut(s) 642, 843, 1167
BstEII GGTNACC 1 cut(s) 797
BstF5I GGATG 5 cut(s) 13, 421, 725, 1317, 1417
BstFNI CGCG 1 cut(s) 504
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 5 cut(s) 485, 774, 1173, 1204, 1266
BstMAI GTCTC 1 cut(s) 905
BstMBI GATC 5 cut(s) 482, 771, 1170, 1201, 1263
BstMWI GCNNNNNNNGC 4 cut(s) 319, 704, 1364, 1373
BstNSI RCATGY 3 cut(s) 147, 918, 1199
BstPAI GACNNNNGTC 1 cut(s) 467
BstPI GGTNACC 1 cut(s) 797
BstSCI CCNGG 1 cut(s) 324
BstSFI CTRYAG 1 cut(s) 1068
BstUI CGCG 1 cut(s) 504
BstV1I GCAGC 2 cut(s) 1351, 1440
BstX2I RGATCY 1 cut(s) 482
BstYI RGATCY 1 cut(s) 482
BsuRI GGCC 2 cut(s) 690, 1376
BtsCI GGATG 5 cut(s) 13, 421, 725, 1317, 1417
BtsI GCAGTG 1 cut(s) 1303
BtsIMutI CAGTG 2 cut(s) 861, 1303
BveI ACCTGC 1 cut(s) 1075
Cac8I GCNNGC 2 cut(s) 506, 1465
CaiI CAGNNNCTG 2 cut(s) 319, 1364
CfoI GCGC 1 cut(s) 46
Cfr13I GGNCC 3 cut(s) 136, 689, 1375
CseI GACGC 1 cut(s) 879
Csp6I GTAC 4 cut(s) 280, 918, 983, 1049
CviAII CATG 9 cut(s) 144, 163, 392, 620, 803, 895, 915, 1196, 1205
CviQI GTAC 4 cut(s) 280, 918, 983, 1049
DdeI CTNAG 3 cut(s) 642, 843, 1167
DpnI GATC 5 cut(s) 484, 773, 1172, 1203, 1265
DpnII GATC 5 cut(s) 482, 771, 1170, 1201, 1263
DraI TTTAAA 1 cut(s) 1255
Eam1104I CTCTTC 1 cut(s) 1296
EarI CTCTTC 1 cut(s) 1296
Eco47I GGWCC 1 cut(s) 136
Eco57I CTGAAG 3 cut(s) 251, 788, 1029
Eco88I CYCGRG 1 cut(s) 275
Eco91I GGTNACC 1 cut(s) 797
EcoO109I RGGNCCY 1 cut(s) 136
EcoO65I GGTNACC 1 cut(s) 797
EcoRI GAATTC 3 cut(s) 216, 604, 880
EcoT22I ATGCAT 2 cut(s) 725, 1252
FaeI CATG 9 cut(s) 147, 166, 395, 623, 806, 898, 918, 1199, 1208
FalI AAGNNNNNCTT 1 cut(s) 1452
FatI CATG 9 cut(s) 143, 162, 391, 619, 802, 894, 914, 1195, 1204
FauI CCCGC 1 cut(s) 122
FauNDI CATATG 1 cut(s) 665
FbaI TGATCA 1 cut(s) 1201
FblI GTMKAC 1 cut(s) 1015
Fnu4HI GCNGC 3 cut(s) 708, 1365, 1454
FokI GGATG 5 cut(s) 20, 428, 732, 1304, 1424
Fsp4HI GCNGC 3 cut(s) 708, 1365, 1454
FspBI CTAG 3 cut(s) 986, 1026, 1244
GlaI GCGC 1 cut(s) 45
GluI GCNGC 3 cut(s) 708, 1365, 1454
GsaI CCCAGC 1 cut(s) 657
GsuI CTGGAG 1 cut(s) 1038
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 2 cut(s) 690, 1376
HapII CCGG 5 cut(s) 209, 326, 749, 860, 1223
HgaI GACGC 1 cut(s) 879
HhaI GCGC 1 cut(s) 46
Hin1II CATG 9 cut(s) 147, 166, 395, 623, 806, 898, 918, 1199, 1208
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HindIII AAGCTT 2 cut(s) 578, 1444
HinfI GANTC 8 cut(s) 52, 188, 271, 316, 456, 938, 944, 1300
HpaII CCGG 5 cut(s) 209, 326, 749, 860, 1223
HphI GGTGA 2 cut(s) 809, 1273
Hpy166II GTNNAC 4 cut(s) 270, 808, 1009, 1016
Hpy8I GTNNAC 4 cut(s) 270, 808, 1009, 1016
HpyAV CCTTC 4 cut(s) 226, 350, 1229, 1366
HpyCH4III ACNGT 5 cut(s) 158, 368, 856, 866, 1013
HpyCH4IV ACGT 2 cut(s) 372, 1113
HpyF10VI GCNNNNNNNGC 4 cut(s) 319, 704, 1364, 1373
HpyF3I CTNAG 3 cut(s) 642, 843, 1167
HpySE526I ACGT 2 cut(s) 372, 1113
Hsp92II CATG 9 cut(s) 147, 166, 395, 623, 806, 898, 918, 1199, 1208
HspAI GCGC 1 cut(s) 44
Kpn2I TCCGGA 2 cut(s) 208, 748
Ksp22I TGATCA 1 cut(s) 1201
Kzo9I GATC 5 cut(s) 482, 771, 1170, 1201, 1263
LmnI GCTCC 4 cut(s) 566, 1131, 1192, 1423
Lsp1109I GCAGC 2 cut(s) 1351, 1440
LweI GCATC 6 cut(s) 710, 732, 814, 877, 1030, 1237
MaeI CTAG 3 cut(s) 986, 1026, 1244
MaeII ACGT 2 cut(s) 372, 1113
MaeIII GTNAC 5 cut(s) 158, 368, 544, 797, 994
MalI GATC 5 cut(s) 484, 773, 1172, 1203, 1265
MboI GATC 5 cut(s) 482, 771, 1170, 1201, 1263
MboII GAAGA 2 cut(s) 781, 1283
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 1 cut(s) 482
MlyI GAGTC 1 cut(s) 265
MmeI TCCRAC 2 cut(s) 216, 460
Mph1103I ATGCAT 2 cut(s) 725, 1252
MroI TCCGGA 2 cut(s) 208, 748
MroXI GAANNNNTTC 2 cut(s) 255, 1290
MspCI CTTAAG 1 cut(s) 1441
MspI CCGG 5 cut(s) 209, 326, 749, 860, 1223
MspR9I CCNGG 1 cut(s) 326
MunI CAATTG 1 cut(s) 465
Mva1269I GAATGC 2 cut(s) 756, 963
MvnI CGCG 1 cut(s) 504
MwoI GCNNNNNNNGC 4 cut(s) 319, 704, 1364, 1373
NciI CCSGG 1 cut(s) 326
NdeI CATATG 1 cut(s) 665
NdeII GATC 5 cut(s) 482, 771, 1170, 1201, 1263
NlaIII CATG 9 cut(s) 147, 166, 395, 623, 806, 898, 918, 1199, 1208
NlaIV GGNNCC 2 cut(s) 138, 338
NmuCI GTSAC 2 cut(s) 368, 797
NsiI ATGCAT 2 cut(s) 725, 1252
NspI RCATGY 3 cut(s) 147, 918, 1199
PacI TTAATTAA 1 cut(s) 1474
PciI ACATGT 2 cut(s) 143, 914
PcsI WCGNNNNNNNCGW 1 cut(s) 55
PctI GAATGC 2 cut(s) 756, 963
PdmI GAANNNNTTC 2 cut(s) 255, 1290
PfeI GAWTC 7 cut(s) 52, 188, 316, 456, 938, 944, 1300
PkrI GCNGC 3 cut(s) 709, 1366, 1455
PleI GAGTC 1 cut(s) 265
PpsI GAGTC 1 cut(s) 265
PpuMI RGGWCCY 1 cut(s) 136
PscI ACATGT 2 cut(s) 143, 914
PshAI GACNNNNGTC 1 cut(s) 467
Psp1406I AACGTT 1 cut(s) 1113
Psp5II RGGWCCY 1 cut(s) 136
PspEI GGTNACC 1 cut(s) 797
PspFI CCCAGC 1 cut(s) 653
PspN4I GGNNCC 2 cut(s) 138, 338
PspPI GGNCC 3 cut(s) 136, 689, 1375
PspPPI RGGWCCY 1 cut(s) 136
PstI CTGCAG 1 cut(s) 1072
PstNI CAGNNNCTG 2 cut(s) 319, 1364
PsuI RGATCY 1 cut(s) 482
RsaI GTAC 4 cut(s) 281, 919, 984, 1050
RsaNI GTAC 4 cut(s) 280, 918, 983, 1049
SatI GCNGC 3 cut(s) 708, 1365, 1454
Sau3AI GATC 5 cut(s) 482, 771, 1170, 1201, 1263
Sau96I GGNCC 3 cut(s) 136, 689, 1375
SchI GAGTC 1 cut(s) 265
ScrFI CCNGG 1 cut(s) 326
SfaNI GCATC 6 cut(s) 710, 732, 814, 877, 1030, 1237
SfcI CTRYAG 1 cut(s) 1068
SinI GGWCC 1 cut(s) 136
SmlI CTYRAG 1 cut(s) 1441
SmoI CTYRAG 1 cut(s) 1441
SsiI CCGC 2 cut(s) 115, 708
SspI AATATT 2 cut(s) 1277, 1395
SspMI CTAG 3 cut(s) 986, 1026, 1244
StyD4I CCNGG 1 cut(s) 324
TaaI ACNGT 5 cut(s) 158, 368, 856, 866, 1013
TaiI ACGT 2 cut(s) 375, 1116
TaqI TCGA 2 cut(s) 82, 649
TauI GCSGC 1 cut(s) 710
TfiI GAWTC 7 cut(s) 52, 188, 316, 456, 938, 944, 1300
TscAI CASTG 2 cut(s) 861, 1310
TseFI GTSAC 2 cut(s) 368, 797
TseI GCWGC 2 cut(s) 1364, 1453
Tsp45I GTSAC 2 cut(s) 368, 797
TspDTI ATGAA 7 cut(s) 240, 332, 636, 749, 1229, 1306, 1388
TspGWI ACGGA 1 cut(s) 146
TspRI CASTG 2 cut(s) 861, 1310
Vha464I CTTAAG 1 cut(s) 1441
VpaK11BI GGWCC 1 cut(s) 136
XapI RAATTY 6 cut(s) 17, 216, 604, 833, 880, 1238
XbaI TCTAGA 1 cut(s) 1243
XceI RCATGY 3 cut(s) 147, 918, 1199
XmiI GTMKAC 1 cut(s) 1015
XmnI GAANNNNTTC 2 cut(s) 255, 1290
XspI CTAG 3 cut(s) 986, 1026, 1244
Zsp2I ATGCAT 2 cut(s) 725, 1252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.