Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G56120 |
| fragaria_vesca | FvH4_1g05380 |
| malus_domestica | MD02G1062400.v1.1 |
| pyrus_communis | pycom02g04360 |
| rosa_chinensis | RchiOBHm_Chr2g0091071 |
| rosa_laevigata | RLG00000016174 |
| rosa_multiflora | Rmu_sc0005171.1_g000009 |
| rosa_roxburghii | Rroxscaffold_2G00150620 |
| rosa_rugosa | Rorug02G0012300 |
| rosa_samantha | Rh2AG058400 Rh2BG056800 Rh2CG059000 Rh2DG058300 |
| rosa_wichuraiana | Rw2G005080 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1368 |
| Acc36I | ACCTGC | 1 cut(s) | 1183 |
| AccB1I | GGYRCC | 1 cut(s) | 1416 |
| AccI | GTMKAC | 1 cut(s) | 546 |
| AccII | CGCG | 1 cut(s) | 60 |
| AccIII | TCCGGA | 1 cut(s) | 697 |
| AclWI | GGATC | 4 cut(s) | 1210, 1246, 1334, 1347 |
| AcsI | RAATTY | 2 cut(s) | 17, 553 |
| AcuI | CTGAAG | 5 cut(s) | 153, 251, 737, 869, 1005 |
| AcvI | CACGTG | 1 cut(s) | 1159 |
| AcyI | GRCGYC | 1 cut(s) | 1365 |
| AfaI | GTAC | 2 cut(s) | 936, 1002 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1378 |
| AflII | CTTAAG | 1 cut(s) | 1393 |
| AflIII | ACRYGT | 1 cut(s) | 621 |
| AgsI | TTSAA | 9 cut(s) | 34, 251, 290, 440, 467, 526, 558, 694, 1190 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 315 |
| AjiI | CACGTC | 1 cut(s) | 25 |
| AjnI | CCWGG | 1 cut(s) | 540 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 545, 577 |
| AluBI | AGCT | 7 cut(s) | 64, 529, 602, 917, 1029, 1076, 1398 |
| AluI | AGCT | 7 cut(s) | 64, 529, 602, 917, 1029, 1076, 1398 |
| Alw21I | GWGCWC | 2 cut(s) | 30, 66 |
| Alw26I | GTCTC | 3 cut(s) | 772, 844, 1072 |
| Alw44I | GTGCAC | 1 cut(s) | 26 |
| AlwI | GGATC | 4 cut(s) | 1210, 1246, 1334, 1347 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 833, 944, 1316 |
| Ama87I | CYCGRG | 1 cut(s) | 312 |
| Aor13HI | TCCGGA | 1 cut(s) | 697 |
| ApaLI | GTGCAC | 1 cut(s) | 26 |
| ApeKI | GCWGC | 1 cut(s) | 656 |
| ApoI | RAATTY | 2 cut(s) | 17, 553 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 255, 351 |
| AspS9I | GGNCC | 4 cut(s) | 181, 278, 638, 1115 |
| AsuC2I | CCSGG | 1 cut(s) | 115 |
| AsuHPI | GGTGA | 1 cut(s) | 872 |
| AsuII | TTCGAA | 1 cut(s) | 49 |
| AsuNHI | GCTAGC | 1 cut(s) | 913 |
| AvaI | CYCGRG | 1 cut(s) | 312 |
| AvaII | GGWCC | 4 cut(s) | 181, 278, 638, 1115 |
| BaeGI | GKGCMC | 4 cut(s) | 30, 46, 965, 1061 |
| BamHI | GGATCC | 1 cut(s) | 1339 |
| BanI | GGYRCC | 1 cut(s) | 1416 |
| BanII | GRGCYC | 1 cut(s) | 66 |
| BbrPI | CACGTG | 1 cut(s) | 1159 |
| BbsI | GAAGAC | 1 cut(s) | 141 |
| Bbv12I | GWGCWC | 2 cut(s) | 30, 66 |
| BbvI | GCAGC | 1 cut(s) | 668 |
| BccI | CCATC | 2 cut(s) | 336, 793 |
| BciT130I | CCWGG | 1 cut(s) | 542 |
| BclI | TGATCA | 1 cut(s) | 1153 |
| BcnI | CCSGG | 1 cut(s) | 115 |
| BcoDI | GTCTC | 3 cut(s) | 772, 844, 1072 |
| BfaI | CTAG | 2 cut(s) | 584, 914 |
| BfmI | CTRYAG | 1 cut(s) | 654 |
| BfrI | CTTAAG | 1 cut(s) | 1393 |
| BfuAI | ACCTGC | 1 cut(s) | 1183 |
| BglII | AGATCT | 1 cut(s) | 1122 |
| BisI | GCNGC | 1 cut(s) | 657 |
| BlsI | GCNGC | 1 cut(s) | 658 |
| Bme1390I | CCNGG | 2 cut(s) | 115, 542 |
| Bme18I | GGWCC | 4 cut(s) | 181, 278, 638, 1115 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 315 |
| BmeT110I | CYCGRG | 1 cut(s) | 312 |
| BmgBI | CACGTC | 1 cut(s) | 25 |
| BmgT120I | GGNCC | 4 cut(s) | 181, 278, 638, 1115 |
| BmiI | GGNNCC | 5 cut(s) | 182, 976, 1093, 1341, 1418 |
| BmrFI | CCNGG | 2 cut(s) | 115, 542 |
| BmsI | GCATC | 5 cut(s) | 659, 715, 763, 931, 1189 |
| BmtI | GCTAGC | 1 cut(s) | 917 |
| BpiI | GAAGAC | 1 cut(s) | 141 |
| BpmI | CTGGAG | 1 cut(s) | 1092 |
| Bpu14I | TTCGAA | 1 cut(s) | 49 |
| BpuMI | CCSGG | 1 cut(s) | 115 |
| Bsa29I | ATCGAT | 1 cut(s) | 723 |
| BsaAI | YACGTR | 1 cut(s) | 1159 |
| BsaHI | GRCGYC | 1 cut(s) | 1365 |
| BsaJI | CCNNGG | 2 cut(s) | 113, 1247 |
| BsaWI | WCCGGW | 1 cut(s) | 697 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1378 |
| Bse1I | ACTGG | 1 cut(s) | 554 |
| Bse3DI | GCAATG | 2 cut(s) | 1255, 1389 |
| BseAI | TCCGGA | 1 cut(s) | 697 |
| BseBI | CCWGG | 1 cut(s) | 542 |
| BseCI | ATCGAT | 1 cut(s) | 723 |
| BseDI | CCNNGG | 2 cut(s) | 113, 1247 |
| BseGI | GGATG | 3 cut(s) | 13, 370, 674 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1378 |
| BseMI | GCAATG | 2 cut(s) | 1255, 1389 |
| BseMII | CTCAG | 3 cut(s) | 165, 605, 1110 |
| BseNI | ACTGG | 1 cut(s) | 554 |
| BseSI | GKGCMC | 4 cut(s) | 30, 46, 965, 1061 |
| BseXI | GCAGC | 1 cut(s) | 668 |
| BseYI | CCCAGC | 1 cut(s) | 602 |
| Bsh1236I | CGCG | 1 cut(s) | 60 |
| Bsh1285I | CGRYCG | 1 cut(s) | 751 |
| BshNI | GGYRCC | 1 cut(s) | 1416 |
| BshVI | ATCGAT | 1 cut(s) | 723 |
| BsiEI | CGRYCG | 1 cut(s) | 751 |
| BsiHKAI | GWGCWC | 2 cut(s) | 30, 66 |
| BsiHKCI | CYCGRG | 1 cut(s) | 312 |
| BsiSI | CCGG | 2 cut(s) | 115, 698 |
| BslFI | GGGAC | 2 cut(s) | 322, 1376 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1378 |
| BsmAI | GTCTC | 3 cut(s) | 772, 844, 1072 |
| BsmBI | CGTCTC | 1 cut(s) | 1072 |
| BsmFI | GGGAC | 2 cut(s) | 322, 1376 |
| BsmI | GAATGC | 3 cut(s) | 705, 991, 1330 |
| BsoBI | CYCGRG | 1 cut(s) | 312 |
| Bsp119I | TTCGAA | 1 cut(s) | 49 |
| Bsp1286I | GDGCHC | 5 cut(s) | 30, 46, 66, 965, 1061 |
| Bsp13I | TCCGGA | 1 cut(s) | 697 |
| Bsp143I | GATC | 8 cut(s) | 369, 720, 748, 1122, 1153, 1215, 1238, 1339 |
| Bsp68I | TCGCGA | 1 cut(s) | 60 |
| BspCNI | CTCAG | 3 cut(s) | 166, 604, 1111 |
| BspDI | ATCGAT | 1 cut(s) | 723 |
| BspEI | TCCGGA | 1 cut(s) | 697 |
| BspFNI | CGCG | 1 cut(s) | 60 |
| BspHI | TCATGA | 1 cut(s) | 340 |
| BspLI | GGNNCC | 5 cut(s) | 182, 976, 1093, 1341, 1418 |
| BspMAI | CTGCAG | 1 cut(s) | 658 |
| BspMI | ACCTGC | 1 cut(s) | 1183 |
| BspOI | GCTAGC | 1 cut(s) | 917 |
| BspPI | GGATC | 4 cut(s) | 1210, 1246, 1334, 1347 |
| BspQI | GCTCTTC | 1 cut(s) | 1191 |
| BspT104I | TTCGAA | 1 cut(s) | 49 |
| BspT107I | GGYRCC | 1 cut(s) | 1416 |
| BspTI | CTTAAG | 1 cut(s) | 1393 |
| BsrDI | GCAATG | 2 cut(s) | 1255, 1389 |
| BsrI | ACTGG | 1 cut(s) | 554 |
| BssECI | CCNNGG | 2 cut(s) | 113, 1247 |
| BssMI | GATC | 8 cut(s) | 369, 720, 748, 1122, 1153, 1215, 1238, 1339 |
| BssNI | GRCGYC | 1 cut(s) | 1365 |
| BssT1I | CCWWGG | 1 cut(s) | 1247 |
| Bst2UI | CCWGG | 1 cut(s) | 542 |
| Bst4CI | ACNGT | 1 cut(s) | 815 |
| Bst6I | CTCTTC | 2 cut(s) | 1191, 1248 |
| BstACI | GRCGYC | 1 cut(s) | 1365 |
| BstAFI | CTTAAG | 1 cut(s) | 1393 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 299, 1316 |
| BstBAI | YACGTR | 1 cut(s) | 1159 |
| BstBI | TTCGAA | 1 cut(s) | 49 |
| BstC8I | GCNNGC | 2 cut(s) | 62, 915 |
| BstDEI | CTNAG | 4 cut(s) | 174, 591, 1101, 1119 |
| BstF5I | GGATG | 3 cut(s) | 13, 370, 674 |
| BstFNI | CGCG | 1 cut(s) | 60 |
| BstKTI | GATC | 8 cut(s) | 372, 723, 751, 1125, 1156, 1218, 1241, 1342 |
| BstMAI | GTCTC | 3 cut(s) | 772, 844, 1072 |
| BstMBI | GATC | 8 cut(s) | 369, 720, 748, 1122, 1153, 1215, 1238, 1339 |
| BstMCI | CGRYCG | 1 cut(s) | 751 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 66, 299, 653, 923, 1316, 1325 |
| BstNI | CCWGG | 1 cut(s) | 542 |
| BstNSI | RCATGY | 3 cut(s) | 147, 625, 1151 |
| BstSCI | CCNGG | 2 cut(s) | 113, 540 |
| BstSFI | CTRYAG | 1 cut(s) | 654 |
| BstSLI | GKGCMC | 4 cut(s) | 30, 46, 965, 1061 |
| BstUI | CGCG | 1 cut(s) | 60 |
| BstV1I | GCAGC | 1 cut(s) | 668 |
| BstV2I | GAAGAC | 1 cut(s) | 141 |
| BstX2I | RGATCY | 3 cut(s) | 1122, 1238, 1339 |
| BstYI | RGATCY | 3 cut(s) | 1122, 1238, 1339 |
| Bsu15I | ATCGAT | 1 cut(s) | 723 |
| BsuTUI | ATCGAT | 1 cut(s) | 723 |
| BtrI | CACGTC | 1 cut(s) | 25 |
| BtsCI | GGATG | 3 cut(s) | 13, 370, 674 |
| BtsIMutI | CAGTG | 2 cut(s) | 845, 1311 |
| BtuMI | TCGCGA | 1 cut(s) | 60 |
| BveI | ACCTGC | 1 cut(s) | 1183 |
| Cac8I | GCNNGC | 2 cut(s) | 62, 915 |
| CaiI | CAGNNNCTG | 3 cut(s) | 833, 944, 1316 |
| CciI | TCATGA | 1 cut(s) | 340 |
| Cfr13I | GGNCC | 4 cut(s) | 181, 278, 638, 1115 |
| ClaI | ATCGAT | 1 cut(s) | 723 |
| CseI | GACGC | 1 cut(s) | 280 |
| Csp6I | GTAC | 2 cut(s) | 935, 1001 |
| CviAII | CATG | 7 cut(s) | 144, 163, 320, 341, 569, 622, 1148 |
| CviQI | GTAC | 2 cut(s) | 935, 1001 |
| DdeI | CTNAG | 4 cut(s) | 174, 591, 1101, 1119 |
| DpnI | GATC | 8 cut(s) | 371, 722, 750, 1124, 1155, 1217, 1240, 1341 |
| DpnII | GATC | 8 cut(s) | 369, 720, 748, 1122, 1153, 1215, 1238, 1339 |
| DriI | GACNNNNNGTC | 1 cut(s) | 315 |
| Eam1104I | CTCTTC | 2 cut(s) | 1191, 1248 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 315 |
| EarI | CTCTTC | 2 cut(s) | 1191, 1248 |
| Ecl136II | GAGCTC | 1 cut(s) | 64 |
| Eco130I | CCWWGG | 1 cut(s) | 1247 |
| Eco24I | GRGCYC | 1 cut(s) | 66 |
| Eco47I | GGWCC | 4 cut(s) | 181, 278, 638, 1115 |
| Eco53kI | GAGCTC | 1 cut(s) | 64 |
| Eco57I | CTGAAG | 5 cut(s) | 153, 251, 737, 869, 1005 |
| Eco72I | CACGTG | 1 cut(s) | 1159 |
| Eco88I | CYCGRG | 1 cut(s) | 312 |
| EcoICRI | GAGCTC | 1 cut(s) | 64 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1115 |
| EcoRI | GAATTC | 1 cut(s) | 553 |
| EcoRII | CCWGG | 1 cut(s) | 540 |
| EcoT14I | CCWWGG | 1 cut(s) | 1247 |
| EcoT22I | ATGCAT | 2 cut(s) | 674, 1204 |
| EcoT38I | GRGCYC | 1 cut(s) | 66 |
| ErhI | CCWWGG | 1 cut(s) | 1247 |
| Esp3I | CGTCTC | 1 cut(s) | 1072 |
| FaeI | CATG | 7 cut(s) | 147, 166, 323, 344, 572, 625, 1151 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 1404 |
| FaqI | GGGAC | 2 cut(s) | 322, 1376 |
| FatI | CATG | 7 cut(s) | 143, 162, 319, 340, 568, 621, 1147 |
| FauNDI | CATATG | 1 cut(s) | 614 |
| FbaI | TGATCA | 1 cut(s) | 1153 |
| FblI | GTMKAC | 1 cut(s) | 546 |
| Fnu4HI | GCNGC | 1 cut(s) | 657 |
| FokI | GGATG | 3 cut(s) | 20, 377, 681 |
| FriOI | GRGCYC | 1 cut(s) | 66 |
| Fsp4HI | GCNGC | 1 cut(s) | 657 |
| FspBI | CTAG | 2 cut(s) | 584, 914 |
| GluI | GCNGC | 1 cut(s) | 657 |
| GsaI | CCCAGC | 1 cut(s) | 606 |
| GsuI | CTGGAG | 1 cut(s) | 1092 |
| HapII | CCGG | 2 cut(s) | 115, 698 |
| HgaI | GACGC | 1 cut(s) | 280 |
| Hin1I | GRCGYC | 1 cut(s) | 1365 |
| Hin1II | CATG | 7 cut(s) | 147, 166, 323, 344, 572, 625, 1151 |
| HincII | GTYRAC | 1 cut(s) | 745 |
| HindII | GTYRAC | 1 cut(s) | 745 |
| HindIII | AAGCTT | 2 cut(s) | 527, 1396 |
| HpaII | CCGG | 2 cut(s) | 115, 698 |
| HphI | GGTGA | 1 cut(s) | 872 |
| Hpy166II | GTNNAC | 3 cut(s) | 28, 547, 745 |
| Hpy8I | GTNNAC | 3 cut(s) | 28, 547, 745 |
| Hpy99I | CGWCG | 2 cut(s) | 26, 1370 |
| HpyAV | CCTTC | 4 cut(s) | 226, 903, 1029, 1406 |
| HpyCH4III | ACNGT | 1 cut(s) | 815 |
| HpyCH4IV | ACGT | 4 cut(s) | 24, 1065, 1158, 1365 |
| HpyCH4V | TGCA | 9 cut(s) | 28, 69, 243, 656, 672, 683, 728, 761, 1202 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 66, 299, 653, 923, 1316, 1325 |
| HpyF3I | CTNAG | 4 cut(s) | 174, 591, 1101, 1119 |
| HpySE526I | ACGT | 4 cut(s) | 24, 1065, 1158, 1365 |
| Hsp92I | GRCGYC | 1 cut(s) | 1365 |
| Hsp92II | CATG | 7 cut(s) | 147, 166, 323, 344, 572, 625, 1151 |
| Kpn2I | TCCGGA | 1 cut(s) | 697 |
| Ksp22I | TGATCA | 1 cut(s) | 1153 |
| Kzo9I | GATC | 8 cut(s) | 369, 720, 748, 1122, 1153, 1215, 1238, 1339 |
| LguI | GCTCTTC | 1 cut(s) | 1191 |
| LmnI | GCTCC | 4 cut(s) | 515, 565, 1073, 1144 |
| Lsp1109I | GCAGC | 1 cut(s) | 668 |
| LweI | GCATC | 5 cut(s) | 659, 715, 763, 931, 1189 |
| MaeI | CTAG | 2 cut(s) | 584, 914 |
| MaeII | ACGT | 4 cut(s) | 24, 1065, 1158, 1365 |
| MaeIII | GTNAC | 4 cut(s) | 158, 493, 617, 860 |
| MalI | GATC | 8 cut(s) | 371, 722, 750, 1124, 1155, 1217, 1240, 1341 |
| MboI | GATC | 8 cut(s) | 369, 720, 748, 1122, 1153, 1215, 1238, 1339 |
| MboII | GAAGA | 6 cut(s) | 146, 730, 862, 865, 1178, 1235 |
| MfeI | CAATTG | 3 cut(s) | 414, 435, 794 |
| MflI | RGATCY | 3 cut(s) | 1122, 1238, 1339 |
| MhlI | GDGCHC | 5 cut(s) | 30, 46, 66, 965, 1061 |
| MlyI | GAGTC | 1 cut(s) | 304 |
| MmeI | TCCRAC | 1 cut(s) | 409 |
| Mph1103I | ATGCAT | 2 cut(s) | 674, 1204 |
| MroI | TCCGGA | 1 cut(s) | 697 |
| MroXI | GAANNNNTTC | 2 cut(s) | 255, 351 |
| MseI | TTAA | 7 cut(s) | 120, 420, 1011, 1134, 1299, 1394, 1426 |
| MspCI | CTTAAG | 1 cut(s) | 1393 |
| MspI | CCGG | 2 cut(s) | 115, 698 |
| MspR9I | CCNGG | 2 cut(s) | 115, 542 |
| MunI | CAATTG | 3 cut(s) | 414, 435, 794 |
| Mva1269I | GAATGC | 3 cut(s) | 705, 991, 1330 |
| MvaI | CCWGG | 1 cut(s) | 542 |
| MvnI | CGCG | 1 cut(s) | 60 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 66, 299, 653, 923, 1316, 1325 |
| NciI | CCSGG | 1 cut(s) | 115 |
| NdeI | CATATG | 1 cut(s) | 614 |
| NdeII | GATC | 8 cut(s) | 369, 720, 748, 1122, 1153, 1215, 1238, 1339 |
| NheI | GCTAGC | 1 cut(s) | 913 |
| NlaIII | CATG | 7 cut(s) | 147, 166, 323, 344, 572, 625, 1151 |
| NlaIV | GGNNCC | 5 cut(s) | 182, 976, 1093, 1341, 1418 |
| NmuCI | GTSAC | 2 cut(s) | 617, 860 |
| NruI | TCGCGA | 1 cut(s) | 60 |
| NsiI | ATGCAT | 2 cut(s) | 674, 1204 |
| NspI | RCATGY | 3 cut(s) | 147, 625, 1151 |
| NspV | TTCGAA | 1 cut(s) | 49 |
| PaeR7I | CTCGAG | 1 cut(s) | 312 |
| PagI | TCATGA | 1 cut(s) | 340 |
| PciI | ACATGT | 1 cut(s) | 621 |
| PciSI | GCTCTTC | 1 cut(s) | 1191 |
| PctI | GAATGC | 3 cut(s) | 705, 991, 1330 |
| PdmI | GAANNNNTTC | 2 cut(s) | 255, 351 |
| PkrI | GCNGC | 1 cut(s) | 658 |
| Ple19I | CGATCG | 1 cut(s) | 751 |
| PleI | GAGTC | 1 cut(s) | 304 |
| PmaCI | CACGTG | 1 cut(s) | 1159 |
| PmlI | CACGTG | 1 cut(s) | 1159 |
| PpsI | GAGTC | 1 cut(s) | 304 |
| Ppu21I | YACGTR | 1 cut(s) | 1159 |
| PpuMI | RGGWCCY | 1 cut(s) | 1115 |
| PscI | ACATGT | 1 cut(s) | 621 |
| Psp124BI | GAGCTC | 1 cut(s) | 66 |
| Psp5II | RGGWCCY | 1 cut(s) | 1115 |
| Psp6I | CCWGG | 1 cut(s) | 540 |
| PspCI | CACGTG | 1 cut(s) | 1159 |
| PspFI | CCCAGC | 1 cut(s) | 602 |
| PspGI | CCWGG | 1 cut(s) | 540 |
| PspN4I | GGNNCC | 5 cut(s) | 182, 976, 1093, 1341, 1418 |
| PspPI | GGNCC | 4 cut(s) | 181, 278, 638, 1115 |
| PspPPI | RGGWCCY | 1 cut(s) | 1115 |
| PspXI | VCTCGAGB | 1 cut(s) | 312 |
| PstI | CTGCAG | 1 cut(s) | 658 |
| PstNI | CAGNNNCTG | 3 cut(s) | 833, 944, 1316 |
| PsuI | RGATCY | 3 cut(s) | 1122, 1238, 1339 |
| PvuI | CGATCG | 1 cut(s) | 751 |
| RruI | TCGCGA | 1 cut(s) | 60 |
| RsaI | GTAC | 2 cut(s) | 936, 1002 |
| RsaNI | GTAC | 2 cut(s) | 935, 1001 |
| SacI | GAGCTC | 1 cut(s) | 66 |
| SapI | GCTCTTC | 1 cut(s) | 1191 |
| SaqAI | TTAA | 7 cut(s) | 120, 420, 1011, 1134, 1299, 1394, 1426 |
| SatI | GCNGC | 1 cut(s) | 657 |
| Sau3AI | GATC | 8 cut(s) | 369, 720, 748, 1122, 1153, 1215, 1238, 1339 |
| Sau96I | GGNCC | 4 cut(s) | 181, 278, 638, 1115 |
| SchI | GAGTC | 1 cut(s) | 304 |
| ScrFI | CCNGG | 2 cut(s) | 115, 542 |
| SduI | GDGCHC | 5 cut(s) | 30, 46, 66, 965, 1061 |
| SfaNI | GCATC | 5 cut(s) | 659, 715, 763, 931, 1189 |
| SfcI | CTRYAG | 1 cut(s) | 654 |
| Sfr274I | CTCGAG | 1 cut(s) | 312 |
| SfuI | TTCGAA | 1 cut(s) | 49 |
| SinI | GGWCC | 4 cut(s) | 181, 278, 638, 1115 |
| SlaI | CTCGAG | 1 cut(s) | 312 |
| SmlI | CTYRAG | 2 cut(s) | 312, 1393 |
| SmoI | CTYRAG | 2 cut(s) | 312, 1393 |
| SspI | AATATT | 4 cut(s) | 337, 769, 1229, 1347 |
| SspMI | CTAG | 2 cut(s) | 584, 914 |
| SstI | GAGCTC | 1 cut(s) | 66 |
| StyD4I | CCNGG | 2 cut(s) | 113, 540 |
| StyI | CCWWGG | 1 cut(s) | 1247 |
| TaaI | ACNGT | 1 cut(s) | 815 |
| TaiI | ACGT | 4 cut(s) | 27, 1068, 1161, 1368 |
| TaqI | TCGA | 7 cut(s) | 21, 49, 82, 313, 598, 723, 921 |
| Tru1I | TTAA | 7 cut(s) | 120, 420, 1011, 1134, 1299, 1394, 1426 |
| Tru9I | TTAA | 7 cut(s) | 120, 420, 1011, 1134, 1299, 1394, 1426 |
| TscAI | CASTG | 2 cut(s) | 852, 1311 |
| TseFI | GTSAC | 2 cut(s) | 617, 860 |
| TseI | GCWGC | 1 cut(s) | 656 |
| Tsp45I | GTSAC | 2 cut(s) | 617, 860 |
| TspGWI | ACGGA | 2 cut(s) | 249, 440 |
| TspRI | CASTG | 2 cut(s) | 852, 1311 |
| Vha464I | CTTAAG | 1 cut(s) | 1393 |
| VneI | GTGCAC | 1 cut(s) | 26 |
| VpaK11BI | GGWCC | 4 cut(s) | 181, 278, 638, 1115 |
| XapI | RAATTY | 2 cut(s) | 17, 553 |
| XceI | RCATGY | 3 cut(s) | 147, 625, 1151 |
| XhoI | CTCGAG | 1 cut(s) | 312 |
| XmiI | GTMKAC | 1 cut(s) | 546 |
| XmnI | GAANNNNTTC | 2 cut(s) | 255, 351 |
| XspI | CTAG | 2 cut(s) | 584, 914 |
| ZraI | GACGTC | 1 cut(s) | 1366 |
| Zsp2I | ATGCAT | 2 cut(s) | 674, 1204 |