Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G56120 |
| fragaria_vesca | FvH4_1g05380 |
| malus_domestica | MD02G1062400.v1.1 |
| pyrus_communis | pycom02g04360 |
| rosa_chinensis | RchiOBHm_Chr2g0091071 |
| rosa_laevigata | RLG00000016174 |
| rosa_multiflora | Rmu_sc0005171.1_g000009 |
| rosa_roxburghii | Rroxscaffold_2G00150620 |
| rosa_rugosa | Rorug02G0012300 |
| rosa_samantha | Rh2AG058400 Rh2BG056800 Rh2CG059000 Rh2DG058300 |
| rosa_wichuraiana | Rw2G005080 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 906 |
| Acc36I | ACCTGC | 1 cut(s) | 1249 |
| AccB1I | GGYRCC | 1 cut(s) | 1482 |
| AccI | GTMKAC | 1 cut(s) | 612 |
| AccII | CGCG | 1 cut(s) | 60 |
| AccIII | TCCGGA | 1 cut(s) | 763 |
| AciI | CCGC | 1 cut(s) | 115 |
| AclWI | GGATC | 6 cut(s) | 1276, 1312, 1312, 1325, 1400, 1413 |
| AcsI | RAATTY | 2 cut(s) | 17, 619 |
| AcuI | CTGAAG | 5 cut(s) | 153, 251, 803, 935, 1071 |
| AcyI | GRCGYC | 1 cut(s) | 903 |
| AfaI | GTAC | 3 cut(s) | 281, 1002, 1068 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1444 |
| AflII | CTTAAG | 1 cut(s) | 1459 |
| AflIII | ACRYGT | 1 cut(s) | 687 |
| AjiI | CACGTC | 1 cut(s) | 25 |
| AjnI | CCWGG | 1 cut(s) | 606 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 611, 643 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 321, 353 |
| AluBI | AGCT | 8 cut(s) | 64, 587, 595, 668, 986, 1095, 1142, 1464 |
| AluI | AGCT | 8 cut(s) | 64, 587, 595, 668, 986, 1095, 1142, 1464 |
| Alw21I | GWGCWC | 2 cut(s) | 30, 66 |
| Alw26I | GTCTC | 2 cut(s) | 838, 910 |
| Alw44I | GTGCAC | 1 cut(s) | 26 |
| AlwI | GGATC | 6 cut(s) | 1276, 1312, 1312, 1325, 1400, 1413 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 899, 1010, 1382 |
| Ama87I | CYCGRG | 1 cut(s) | 332 |
| Aor13HI | TCCGGA | 1 cut(s) | 763 |
| ApaLI | GTGCAC | 1 cut(s) | 26 |
| ApeKI | GCWGC | 3 cut(s) | 584, 722, 983 |
| ApoI | RAATTY | 2 cut(s) | 17, 619 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 255, 417 |
| AspS9I | GGNCC | 3 cut(s) | 181, 704, 1181 |
| AsuC2I | CCSGG | 1 cut(s) | 327 |
| AsuHPI | GGTGA | 1 cut(s) | 938 |
| AsuII | TTCGAA | 1 cut(s) | 49 |
| AsuNHI | GCTAGC | 1 cut(s) | 979 |
| AvaI | CYCGRG | 1 cut(s) | 332 |
| AvaII | GGWCC | 3 cut(s) | 181, 704, 1181 |
| BaeGI | GKGCMC | 2 cut(s) | 30, 46 |
| BamHI | GGATCC | 2 cut(s) | 1317, 1405 |
| BanI | GGYRCC | 1 cut(s) | 1482 |
| BanII | GRGCYC | 1 cut(s) | 66 |
| BbsI | GAAGAC | 1 cut(s) | 141 |
| Bbv12I | GWGCWC | 2 cut(s) | 30, 66 |
| BbvCI | CCTCAGC | 1 cut(s) | 987 |
| BbvI | GCAGC | 3 cut(s) | 596, 734, 995 |
| BccI | CCATC | 4 cut(s) | 402, 859, 938, 1115 |
| BciT130I | CCWGG | 1 cut(s) | 608 |
| BclI | TGATCA | 1 cut(s) | 1219 |
| BcnI | CCSGG | 1 cut(s) | 327 |
| BcoDI | GTCTC | 2 cut(s) | 838, 910 |
| BfaI | CTAG | 2 cut(s) | 650, 980 |
| BfmI | CTRYAG | 2 cut(s) | 720, 1137 |
| BfrI | CTTAAG | 1 cut(s) | 1459 |
| BfuAI | ACCTGC | 1 cut(s) | 1249 |
| BglII | AGATCT | 1 cut(s) | 1188 |
| BisI | GCNGC | 3 cut(s) | 585, 723, 984 |
| BlsI | GCNGC | 3 cut(s) | 586, 724, 985 |
| Bme1390I | CCNGG | 2 cut(s) | 327, 608 |
| Bme18I | GGWCC | 3 cut(s) | 181, 704, 1181 |
| BmeT110I | CYCGRG | 1 cut(s) | 332 |
| BmgBI | CACGTC | 1 cut(s) | 25 |
| BmgT120I | GGNCC | 3 cut(s) | 181, 704, 1181 |
| BmiI | GGNNCC | 7 cut(s) | 182, 339, 1042, 1159, 1319, 1407, 1484 |
| BmrFI | CCNGG | 2 cut(s) | 327, 608 |
| BmsI | GCATC | 4 cut(s) | 725, 829, 997, 1255 |
| BmtI | GCTAGC | 1 cut(s) | 983 |
| BpiI | GAAGAC | 1 cut(s) | 141 |
| Bpu10I | CCTNAGC | 1 cut(s) | 987 |
| Bpu14I | TTCGAA | 1 cut(s) | 49 |
| BpuMI | CCSGG | 1 cut(s) | 327 |
| BsaBI | GATNNNNATC | 1 cut(s) | 948 |
| BsaHI | GRCGYC | 1 cut(s) | 903 |
| BsaJI | CCNNGG | 1 cut(s) | 1313 |
| BsaWI | WCCGGW | 1 cut(s) | 763 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1444 |
| Bse1I | ACTGG | 1 cut(s) | 620 |
| Bse3DI | GCAATG | 2 cut(s) | 1321, 1455 |
| Bse8I | GATNNNNATC | 1 cut(s) | 948 |
| BseAI | TCCGGA | 1 cut(s) | 763 |
| BseBI | CCWGG | 1 cut(s) | 608 |
| BseDI | CCNNGG | 1 cut(s) | 1313 |
| BseGI | GGATG | 4 cut(s) | 13, 436, 740, 1435 |
| BseJI | GATNNNNATC | 1 cut(s) | 948 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1444 |
| BseMI | GCAATG | 2 cut(s) | 1321, 1455 |
| BseMII | CTCAG | 4 cut(s) | 165, 671, 978, 1176 |
| BseNI | ACTGG | 1 cut(s) | 620 |
| BseSI | GKGCMC | 2 cut(s) | 30, 46 |
| BseXI | GCAGC | 3 cut(s) | 596, 734, 995 |
| BseYI | CCCAGC | 1 cut(s) | 668 |
| BsgI | GTGCAG | 1 cut(s) | 315 |
| Bsh1236I | CGCG | 1 cut(s) | 60 |
| Bsh1285I | CGRYCG | 1 cut(s) | 817 |
| BshNI | GGYRCC | 1 cut(s) | 1482 |
| BsiEI | CGRYCG | 1 cut(s) | 817 |
| BsiHKAI | GWGCWC | 2 cut(s) | 30, 66 |
| BsiHKCI | CYCGRG | 1 cut(s) | 332 |
| BsiSI | CCGG | 2 cut(s) | 326, 764 |
| BslFI | GGGAC | 1 cut(s) | 342 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1444 |
| BsmAI | GTCTC | 2 cut(s) | 838, 910 |
| BsmBI | CGTCTC | 1 cut(s) | 910 |
| BsmFI | GGGAC | 1 cut(s) | 342 |
| BsmI | GAATGC | 3 cut(s) | 771, 1057, 1396 |
| BsoBI | CYCGRG | 1 cut(s) | 332 |
| Bsp119I | TTCGAA | 1 cut(s) | 49 |
| Bsp1286I | GDGCHC | 3 cut(s) | 30, 46, 66 |
| Bsp13I | TCCGGA | 1 cut(s) | 763 |
| Bsp143I | GATC | 9 cut(s) | 435, 786, 814, 1188, 1219, 1281, 1304, 1317, 1405 |
| Bsp68I | TCGCGA | 1 cut(s) | 60 |
| BspACI | CCGC | 1 cut(s) | 115 |
| BspCNI | CTCAG | 4 cut(s) | 166, 670, 979, 1177 |
| BspEI | TCCGGA | 1 cut(s) | 763 |
| BspFNI | CGCG | 1 cut(s) | 60 |
| BspHI | TCATGA | 1 cut(s) | 406 |
| BspLI | GGNNCC | 7 cut(s) | 182, 339, 1042, 1159, 1319, 1407, 1484 |
| BspMAI | CTGCAG | 1 cut(s) | 724 |
| BspMI | ACCTGC | 1 cut(s) | 1249 |
| BspOI | GCTAGC | 1 cut(s) | 983 |
| BspPI | GGATC | 6 cut(s) | 1276, 1312, 1312, 1325, 1400, 1413 |
| BspQI | GCTCTTC | 1 cut(s) | 1257 |
| BspT104I | TTCGAA | 1 cut(s) | 49 |
| BspT107I | GGYRCC | 1 cut(s) | 1482 |
| BspTI | CTTAAG | 1 cut(s) | 1459 |
| BsrDI | GCAATG | 2 cut(s) | 1321, 1455 |
| BsrI | ACTGG | 1 cut(s) | 620 |
| BssECI | CCNNGG | 1 cut(s) | 1313 |
| BssMI | GATC | 9 cut(s) | 435, 786, 814, 1188, 1219, 1281, 1304, 1317, 1405 |
| BssNI | GRCGYC | 1 cut(s) | 903 |
| BssT1I | CCWWGG | 1 cut(s) | 1313 |
| Bst2UI | CCWGG | 1 cut(s) | 608 |
| Bst4CI | ACNGT | 3 cut(s) | 370, 383, 881 |
| Bst6I | CTCTTC | 2 cut(s) | 1257, 1314 |
| BstACI | GRCGYC | 1 cut(s) | 903 |
| BstAFI | CTTAAG | 1 cut(s) | 1459 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 319, 1382 |
| BstBI | TTCGAA | 1 cut(s) | 49 |
| BstC8I | GCNNGC | 2 cut(s) | 62, 981 |
| BstDEI | CTNAG | 5 cut(s) | 174, 657, 987, 1167, 1185 |
| BstF5I | GGATG | 4 cut(s) | 13, 436, 740, 1435 |
| BstFNI | CGCG | 1 cut(s) | 60 |
| BstKTI | GATC | 9 cut(s) | 438, 789, 817, 1191, 1222, 1284, 1307, 1320, 1408 |
| BstMAI | GTCTC | 2 cut(s) | 838, 910 |
| BstMBI | GATC | 9 cut(s) | 435, 786, 814, 1188, 1219, 1281, 1304, 1317, 1405 |
| BstMCI | CGRYCG | 1 cut(s) | 817 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 66, 319, 719, 989, 1382, 1391 |
| BstNI | CCWGG | 1 cut(s) | 608 |
| BstNSI | RCATGY | 3 cut(s) | 147, 691, 1217 |
| BstSCI | CCNGG | 2 cut(s) | 325, 606 |
| BstSFI | CTRYAG | 2 cut(s) | 720, 1137 |
| BstSLI | GKGCMC | 2 cut(s) | 30, 46 |
| BstUI | CGCG | 1 cut(s) | 60 |
| BstV1I | GCAGC | 3 cut(s) | 596, 734, 995 |
| BstV2I | GAAGAC | 1 cut(s) | 141 |
| BstX2I | RGATCY | 4 cut(s) | 1188, 1304, 1317, 1405 |
| BstYI | RGATCY | 4 cut(s) | 1188, 1304, 1317, 1405 |
| BtrI | CACGTC | 1 cut(s) | 25 |
| BtsCI | GGATG | 4 cut(s) | 13, 436, 740, 1435 |
| BtsIMutI | CAGTG | 1 cut(s) | 1377 |
| BtuMI | TCGCGA | 1 cut(s) | 60 |
| BveI | ACCTGC | 1 cut(s) | 1249 |
| Cac8I | GCNNGC | 2 cut(s) | 62, 981 |
| CaiI | CAGNNNCTG | 3 cut(s) | 899, 1010, 1382 |
| CciI | TCATGA | 1 cut(s) | 406 |
| Cfr13I | GGNCC | 3 cut(s) | 181, 704, 1181 |
| Csp6I | GTAC | 3 cut(s) | 280, 1001, 1067 |
| CviAII | CATG | 8 cut(s) | 144, 163, 386, 407, 635, 688, 1214, 1225 |
| CviQI | GTAC | 3 cut(s) | 280, 1001, 1067 |
| DdeI | CTNAG | 5 cut(s) | 174, 657, 987, 1167, 1185 |
| DpnI | GATC | 9 cut(s) | 437, 788, 816, 1190, 1221, 1283, 1306, 1319, 1407 |
| DpnII | GATC | 9 cut(s) | 435, 786, 814, 1188, 1219, 1281, 1304, 1317, 1405 |
| Eam1104I | CTCTTC | 2 cut(s) | 1257, 1314 |
| EarI | CTCTTC | 2 cut(s) | 1257, 1314 |
| Ecl136II | GAGCTC | 1 cut(s) | 64 |
| Eco130I | CCWWGG | 1 cut(s) | 1313 |
| Eco24I | GRGCYC | 1 cut(s) | 66 |
| Eco47I | GGWCC | 3 cut(s) | 181, 704, 1181 |
| Eco53kI | GAGCTC | 1 cut(s) | 64 |
| Eco57I | CTGAAG | 5 cut(s) | 153, 251, 803, 935, 1071 |
| Eco88I | CYCGRG | 1 cut(s) | 332 |
| EcoICRI | GAGCTC | 1 cut(s) | 64 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1181 |
| EcoRI | GAATTC | 1 cut(s) | 619 |
| EcoRII | CCWGG | 1 cut(s) | 606 |
| EcoT14I | CCWWGG | 1 cut(s) | 1313 |
| EcoT22I | ATGCAT | 2 cut(s) | 740, 1270 |
| EcoT38I | GRGCYC | 1 cut(s) | 66 |
| ErhI | CCWWGG | 1 cut(s) | 1313 |
| Esp3I | CGTCTC | 1 cut(s) | 910 |
| FaeI | CATG | 8 cut(s) | 147, 166, 389, 410, 638, 691, 1217, 1228 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 1470 |
| FaqI | GGGAC | 1 cut(s) | 342 |
| FatI | CATG | 8 cut(s) | 143, 162, 385, 406, 634, 687, 1213, 1224 |
| FauI | CCCGC | 1 cut(s) | 122 |
| FauNDI | CATATG | 1 cut(s) | 680 |
| FbaI | TGATCA | 1 cut(s) | 1219 |
| FblI | GTMKAC | 1 cut(s) | 612 |
| Fnu4HI | GCNGC | 3 cut(s) | 585, 723, 984 |
| FokI | GGATG | 4 cut(s) | 20, 443, 747, 1442 |
| FriOI | GRGCYC | 1 cut(s) | 66 |
| Fsp4HI | GCNGC | 3 cut(s) | 585, 723, 984 |
| FspBI | CTAG | 2 cut(s) | 650, 980 |
| GluI | GCNGC | 3 cut(s) | 585, 723, 984 |
| GsaI | CCCAGC | 1 cut(s) | 672 |
| HapII | CCGG | 2 cut(s) | 326, 764 |
| Hin1I | GRCGYC | 1 cut(s) | 903 |
| Hin1II | CATG | 8 cut(s) | 147, 166, 389, 410, 638, 691, 1217, 1228 |
| HincII | GTYRAC | 1 cut(s) | 811 |
| HindII | GTYRAC | 1 cut(s) | 811 |
| HindIII | AAGCTT | 2 cut(s) | 593, 1462 |
| HpaII | CCGG | 2 cut(s) | 326, 764 |
| HphI | GGTGA | 1 cut(s) | 938 |
| Hpy166II | GTNNAC | 4 cut(s) | 28, 613, 811, 1027 |
| Hpy8I | GTNNAC | 4 cut(s) | 28, 613, 811, 1027 |
| Hpy99I | CGWCG | 1 cut(s) | 26 |
| HpyAV | CCTTC | 6 cut(s) | 226, 351, 724, 969, 1095, 1472 |
| HpyCH4III | ACNGT | 3 cut(s) | 370, 383, 881 |
| HpyCH4IV | ACGT | 2 cut(s) | 24, 903 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 66, 319, 719, 989, 1382, 1391 |
| HpyF3I | CTNAG | 5 cut(s) | 174, 657, 987, 1167, 1185 |
| HpySE526I | ACGT | 2 cut(s) | 24, 903 |
| Hsp92I | GRCGYC | 1 cut(s) | 903 |
| Hsp92II | CATG | 8 cut(s) | 147, 166, 389, 410, 638, 691, 1217, 1228 |
| Kpn2I | TCCGGA | 1 cut(s) | 763 |
| Ksp22I | TGATCA | 1 cut(s) | 1219 |
| Kzo9I | GATC | 9 cut(s) | 435, 786, 814, 1188, 1219, 1281, 1304, 1317, 1405 |
| LguI | GCTCTTC | 1 cut(s) | 1257 |
| LmnI | GCTCC | 3 cut(s) | 581, 631, 1210 |
| Lsp1109I | GCAGC | 3 cut(s) | 596, 734, 995 |
| LweI | GCATC | 4 cut(s) | 725, 829, 997, 1255 |
| MaeI | CTAG | 2 cut(s) | 650, 980 |
| MaeII | ACGT | 2 cut(s) | 24, 903 |
| MaeIII | GTNAC | 4 cut(s) | 377, 559, 683, 926 |
| MalI | GATC | 9 cut(s) | 437, 788, 816, 1190, 1221, 1283, 1306, 1319, 1407 |
| MboI | GATC | 9 cut(s) | 435, 786, 814, 1188, 1219, 1281, 1304, 1317, 1405 |
| MboII | GAAGA | 6 cut(s) | 146, 796, 928, 931, 1244, 1301 |
| MfeI | CAATTG | 3 cut(s) | 480, 501, 860 |
| MflI | RGATCY | 4 cut(s) | 1188, 1304, 1317, 1405 |
| MhlI | GDGCHC | 3 cut(s) | 30, 46, 66 |
| MlyI | GAGTC | 1 cut(s) | 324 |
| MmeI | TCCRAC | 1 cut(s) | 475 |
| MnlI | CCTC | 7 cut(s) | 66, 171, 328, 333, 982, 1080, 1172 |
| Mph1103I | ATGCAT | 2 cut(s) | 740, 1270 |
| MroI | TCCGGA | 1 cut(s) | 763 |
| MroXI | GAANNNNTTC | 2 cut(s) | 255, 417 |
| MseI | TTAA | 8 cut(s) | 120, 365, 486, 1077, 1200, 1365, 1460, 1492 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1229 |
| MspA1I | CMGCKG | 1 cut(s) | 986 |
| MspCI | CTTAAG | 1 cut(s) | 1459 |
| MspI | CCGG | 2 cut(s) | 326, 764 |
| MspR9I | CCNGG | 2 cut(s) | 327, 608 |
| MunI | CAATTG | 3 cut(s) | 480, 501, 860 |
| Mva1269I | GAATGC | 3 cut(s) | 771, 1057, 1396 |
| MvaI | CCWGG | 1 cut(s) | 608 |
| MvnI | CGCG | 1 cut(s) | 60 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 66, 319, 719, 989, 1382, 1391 |
| NciI | CCSGG | 1 cut(s) | 327 |
| NdeI | CATATG | 1 cut(s) | 680 |
| NdeII | GATC | 9 cut(s) | 435, 786, 814, 1188, 1219, 1281, 1304, 1317, 1405 |
| NheI | GCTAGC | 1 cut(s) | 979 |
| NlaIII | CATG | 8 cut(s) | 147, 166, 389, 410, 638, 691, 1217, 1228 |
| NlaIV | GGNNCC | 7 cut(s) | 182, 339, 1042, 1159, 1319, 1407, 1484 |
| NmuCI | GTSAC | 2 cut(s) | 683, 926 |
| NruI | TCGCGA | 1 cut(s) | 60 |
| NsiI | ATGCAT | 2 cut(s) | 740, 1270 |
| NspI | RCATGY | 3 cut(s) | 147, 691, 1217 |
| NspV | TTCGAA | 1 cut(s) | 49 |
| PaeR7I | CTCGAG | 1 cut(s) | 332 |
| PagI | TCATGA | 1 cut(s) | 406 |
| PciI | ACATGT | 1 cut(s) | 687 |
| PciSI | GCTCTTC | 1 cut(s) | 1257 |
| PctI | GAATGC | 3 cut(s) | 771, 1057, 1396 |
| PdmI | GAANNNNTTC | 2 cut(s) | 255, 417 |
| PfoI | TCCNGGA | 1 cut(s) | 325 |
| PkrI | GCNGC | 3 cut(s) | 586, 724, 985 |
| Ple19I | CGATCG | 1 cut(s) | 817 |
| PleI | GAGTC | 1 cut(s) | 324 |
| PpsI | GAGTC | 1 cut(s) | 324 |
| PpuMI | RGGWCCY | 1 cut(s) | 1181 |
| PscI | ACATGT | 1 cut(s) | 687 |
| Psp124BI | GAGCTC | 1 cut(s) | 66 |
| Psp5II | RGGWCCY | 1 cut(s) | 1181 |
| Psp6I | CCWGG | 1 cut(s) | 606 |
| PspFI | CCCAGC | 1 cut(s) | 668 |
| PspGI | CCWGG | 1 cut(s) | 606 |
| PspN4I | GGNNCC | 7 cut(s) | 182, 339, 1042, 1159, 1319, 1407, 1484 |
| PspPI | GGNCC | 3 cut(s) | 181, 704, 1181 |
| PspPPI | RGGWCCY | 1 cut(s) | 1181 |
| PspXI | VCTCGAGB | 1 cut(s) | 332 |
| PstI | CTGCAG | 1 cut(s) | 724 |
| PstNI | CAGNNNCTG | 3 cut(s) | 899, 1010, 1382 |
| PsuI | RGATCY | 4 cut(s) | 1188, 1304, 1317, 1405 |
| PvuI | CGATCG | 1 cut(s) | 817 |
| PvuII | CAGCTG | 1 cut(s) | 986 |
| RruI | TCGCGA | 1 cut(s) | 60 |
| RsaI | GTAC | 3 cut(s) | 281, 1002, 1068 |
| RsaNI | GTAC | 3 cut(s) | 280, 1001, 1067 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1229 |
| SacI | GAGCTC | 1 cut(s) | 66 |
| SapI | GCTCTTC | 1 cut(s) | 1257 |
| SaqAI | TTAA | 8 cut(s) | 120, 365, 486, 1077, 1200, 1365, 1460, 1492 |
| SatI | GCNGC | 3 cut(s) | 585, 723, 984 |
| Sau3AI | GATC | 9 cut(s) | 435, 786, 814, 1188, 1219, 1281, 1304, 1317, 1405 |
| Sau96I | GGNCC | 3 cut(s) | 181, 704, 1181 |
| SchI | GAGTC | 1 cut(s) | 324 |
| ScrFI | CCNGG | 2 cut(s) | 327, 608 |
| SduI | GDGCHC | 3 cut(s) | 30, 46, 66 |
| SfaNI | GCATC | 4 cut(s) | 725, 829, 997, 1255 |
| SfcI | CTRYAG | 2 cut(s) | 720, 1137 |
| Sfr274I | CTCGAG | 1 cut(s) | 332 |
| SfuI | TTCGAA | 1 cut(s) | 49 |
| SinI | GGWCC | 3 cut(s) | 181, 704, 1181 |
| SlaI | CTCGAG | 1 cut(s) | 332 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1229 |
| SmlI | CTYRAG | 2 cut(s) | 332, 1459 |
| SmoI | CTYRAG | 2 cut(s) | 332, 1459 |
| SsiI | CCGC | 1 cut(s) | 115 |
| SspI | AATATT | 4 cut(s) | 403, 835, 1295, 1413 |
| SspMI | CTAG | 2 cut(s) | 650, 980 |
| SstI | GAGCTC | 1 cut(s) | 66 |
| StyD4I | CCNGG | 2 cut(s) | 325, 606 |
| StyI | CCWWGG | 1 cut(s) | 1313 |
| TaaI | ACNGT | 3 cut(s) | 370, 383, 881 |
| TaiI | ACGT | 2 cut(s) | 27, 906 |
| TaqI | TCGA | 5 cut(s) | 21, 49, 82, 333, 664 |
| Tru1I | TTAA | 8 cut(s) | 120, 365, 486, 1077, 1200, 1365, 1460, 1492 |
| Tru9I | TTAA | 8 cut(s) | 120, 365, 486, 1077, 1200, 1365, 1460, 1492 |
| TscAI | CASTG | 1 cut(s) | 1377 |
| TseFI | GTSAC | 2 cut(s) | 683, 926 |
| TseI | GCWGC | 3 cut(s) | 584, 722, 983 |
| Tsp45I | GTSAC | 2 cut(s) | 683, 926 |
| TspGWI | ACGGA | 2 cut(s) | 108, 506 |
| TspRI | CASTG | 1 cut(s) | 1377 |
| Vha464I | CTTAAG | 1 cut(s) | 1459 |
| VneI | GTGCAC | 1 cut(s) | 26 |
| VpaK11BI | GGWCC | 3 cut(s) | 181, 704, 1181 |
| XapI | RAATTY | 2 cut(s) | 17, 619 |
| XceI | RCATGY | 3 cut(s) | 147, 691, 1217 |
| XhoI | CTCGAG | 1 cut(s) | 332 |
| XmiI | GTMKAC | 1 cut(s) | 612 |
| XmnI | GAANNNNTTC | 2 cut(s) | 255, 417 |
| XspI | CTAG | 2 cut(s) | 650, 980 |
| ZraI | GACGTC | 1 cut(s) | 904 |
| Zsp2I | ATGCAT | 2 cut(s) | 740, 1270 |