FvH4_1g09860

N-acylphosphatidylethanolamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0006839
Physical Location & Seq
Reverse (-)
5364336 .. 5366758
2423 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g09860.t2

Sequence Viewer

Length: 864 bp
ATGGCGCGAAGAATAATGGAATGGGCGGGTCGACCCGACTACATGGGCGGCATTCCAAGAAAGATAGTGATCGGAGTCGTCGGAACCTTCGCGAAAACCTGGGTCAACCTTCTCAACTCCACCACCGTTCACAATGCCGATACGCTTCTCAACCTCGTCCGCTCTCGCCCTAAGGGCCTCCCTCTCATCACCGTCAGCAACCACATGTCGACTCTGGACGACCCTCTTCTGTGGGGCATCAAGGGCTTTCCGATTTGTGATGCCAATTTGACGCGGTGGGTTTTGGCCGCCGAAGATATCTGCTTTAAAAGCTCGCCGCTTTCGTACTTTTTCCGGCTAGGGAAGTGTATACCGATTACGAGAGGCGATGGGATCTATCAGGAGCATATGAATGAAGCTCTTGAGAGATTGAGTGAAGGAGATTGGTTGCACACTTTCCCTGAAGGGAAGGTGTACCAGGAAGATGCACCTATAAGAAGATTGAAATGGGGGACTGCTAGTCTCATTGCTCGCGCTCCAGTAACCCCAATAGTTTTGCCAATTGTACATAGAGGGTTCGACAAGGTGATGCCTGATAAATCTTCCCGTGGTAAAAGGCCTCCCTTTCCATTGCTTAATAAGGAAATCAAGATATTTATTGGTGAGCCGATGAAATTTGACATTCCTAAAATGAAGCAGGTGGCTATCTCTATGTCTCCTAAAACGTCACATCCTTCTTTGGGATGGCCAAGCCTTTGCCCAGGTGGTTTGGATGAGGCAGCACAAAGATGCCTGTACAGTGCAATATCAGAGAAAATTCAAAATGCCTTGGAGAGATTACGAATTTTTGGTACAAGTTATAAAAAAGATCGAAATGCATGCTAG

Protein Analysis

288

Amino Acids

32.24

Weight (kDa)

9.58

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransferase PF01553 56 - 182 1.4e-17 Acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 840
AarI CACCTGC 1 cut(s) 667
Acc36I ACCTGC 1 cut(s) 667
AccBSI CCGCTC 1 cut(s) 162
AccI GTMKAC 3 cut(s) 31, 209, 349
AccII CGCG 4 cut(s) 7, 92, 274, 513
AciI CCGC 6 cut(s) 26, 48, 160, 274, 288, 317
AclWI GGATC 1 cut(s) 380
AcoI YGGCCR 2 cut(s) 285, 725
AcsI RAATTY 3 cut(s) 653, 795, 822
AcuI CTGAAG 1 cut(s) 462
AfaI GTAC 5 cut(s) 326, 455, 546, 776, 832
AfiI CCNNNNNNNGG 1 cut(s) 719
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 2 cut(s) 484, 800
AhdI GACNNNNNGTC 1 cut(s) 498
AjnI CCWGG 3 cut(s) 98, 456, 739
AluBI AGCT 2 cut(s) 312, 398
AluI AGCT 2 cut(s) 312, 398
Alw26I GTCTC 2 cut(s) 506, 699
AlwI GGATC 1 cut(s) 380
AoxI GGCC 4 cut(s) 175, 285, 596, 725
ApeKI GCWGC 1 cut(s) 758
ApoI RAATTY 3 cut(s) 653, 795, 822
AspLEI GCGC 2 cut(s) 7, 515
AspS9I GGNCC 1 cut(s) 175
AsuHPI GGTGA 3 cut(s) 181, 577, 653
AxyI CCTNAGG 1 cut(s) 171
BalI TGGCCA 1 cut(s) 727
BbvI GCAGC 1 cut(s) 770
BccI CCATC 2 cut(s) 362, 717
BcgI CGANNNNNNTGC 1 cut(s) 840
BciT130I CCWGG 3 cut(s) 100, 458, 741
BcoDI GTCTC 2 cut(s) 506, 699
BfaI CTAG 3 cut(s) 338, 498, 862
BfuAI ACCTGC 1 cut(s) 667
BglI GCCNNNNNGGC 1 cut(s) 174
BisI GCNGC 4 cut(s) 49, 288, 317, 759
BlsI GCNGC 4 cut(s) 50, 289, 318, 760
Bme1390I CCNGG 3 cut(s) 100, 458, 741
BmeRI GACNNNNNGTC 1 cut(s) 498
BmgT120I GGNCC 1 cut(s) 175
BmiI GGNNCC 1 cut(s) 85
BmrFI CCNGG 3 cut(s) 100, 458, 741
BmsI GCATC 5 cut(s) 246, 250, 454, 558, 758
BpmI CTGGAG 1 cut(s) 501
BpuEI CTTGAG 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 68
BsaJI CCNNGG 4 cut(s) 99, 586, 739, 807
Bsc4I CCNNNNNNNGG 1 cut(s) 719
Bse1I ACTGG 1 cut(s) 518
Bse21I CCTNAGG 1 cut(s) 171
Bse3DI GCAATG 2 cut(s) 504, 608
Bse8I GATNNNNATC 1 cut(s) 68
BseBI CCWGG 3 cut(s) 100, 458, 741
BseDI CCNNGG 4 cut(s) 99, 586, 739, 807
BseGI GGATG 3 cut(s) 709, 728, 757
BseJI GATNNNNATC 1 cut(s) 68
BseLI CCNNNNNNNGG 1 cut(s) 719
BseMI GCAATG 2 cut(s) 504, 608
BseNI ACTGG 1 cut(s) 518
BseXI GCAGC 1 cut(s) 770
Bsh1236I CGCG 4 cut(s) 7, 92, 274, 513
BshFI GGCC 4 cut(s) 177, 287, 598, 727
BsiSI CCGG 1 cut(s) 334
BslFI GGGAC 1 cut(s) 505
BslI CCNNNNNNNGG 1 cut(s) 719
BsmAI GTCTC 2 cut(s) 506, 699
BsmFI GGGAC 1 cut(s) 505
BsmI GAATGC 1 cut(s) 51
BsnI GGCC 4 cut(s) 177, 287, 598, 727
Bsp1407I TGTACA 2 cut(s) 544, 774
Bsp143I GATC 3 cut(s) 69, 372, 847
Bsp68I TCGCGA 1 cut(s) 92
BspACI CCGC 6 cut(s) 26, 48, 160, 274, 288, 317
BspANI GGCC 4 cut(s) 177, 287, 598, 727
BspFNI CGCG 4 cut(s) 7, 92, 274, 513
BspLI GGNNCC 1 cut(s) 85
BspMI ACCTGC 1 cut(s) 667
BspPI GGATC 1 cut(s) 380
BsrBI CCGCTC 1 cut(s) 162
BsrDI GCAATG 2 cut(s) 504, 608
BsrGI TGTACA 2 cut(s) 544, 774
BsrI ACTGG 1 cut(s) 518
BssECI CCNNGG 4 cut(s) 99, 586, 739, 807
BssMI GATC 3 cut(s) 69, 372, 847
BssNAI GTATAC 1 cut(s) 350
BssT1I CCWWGG 1 cut(s) 807
Bst1107I GTATAC 1 cut(s) 350
Bst2UI CCWGG 3 cut(s) 100, 458, 741
Bst4CI ACNGT 3 cut(s) 127, 193, 779
Bst6I CTCTTC 1 cut(s) 231
BstAUI TGTACA 2 cut(s) 544, 774
BstC8I GCNNGC 3 cut(s) 314, 511, 859
BstDEI CTNAG 1 cut(s) 171
BstDSI CCRYGG 1 cut(s) 586
BstF5I GGATG 3 cut(s) 709, 728, 757
BstFNI CGCG 4 cut(s) 7, 92, 274, 513
BstHHI GCGC 2 cut(s) 7, 515
BstKTI GATC 3 cut(s) 72, 375, 850
BstMAI GTCTC 2 cut(s) 506, 699
BstMBI GATC 3 cut(s) 69, 372, 847
BstMWI GCNNNNNNNGC 3 cut(s) 174, 243, 309
BstNI CCWGG 3 cut(s) 100, 458, 741
BstNSI RCATGY 2 cut(s) 208, 861
BstSCI CCNGG 3 cut(s) 98, 456, 739
BstUI CGCG 4 cut(s) 7, 92, 274, 513
BstV1I GCAGC 1 cut(s) 770
BstX2I RGATCY 1 cut(s) 372
BstYI RGATCY 1 cut(s) 372
BstZ17I GTATAC 1 cut(s) 350
Bsu36I CCTNAGG 1 cut(s) 171
BsuRI GGCC 4 cut(s) 177, 287, 598, 727
BtgI CCRYGG 1 cut(s) 586
BtgZI GCGATG 1 cut(s) 381
BtsCI GGATG 3 cut(s) 709, 728, 757
BtsIMutI CAGTG 1 cut(s) 784
BtuMI TCGCGA 1 cut(s) 92
BveI ACCTGC 1 cut(s) 667
Cac8I GCNNGC 3 cut(s) 314, 511, 859
CfoI GCGC 2 cut(s) 7, 515
Cfr13I GGNCC 1 cut(s) 175
CseI GACGC 1 cut(s) 280
Csp6I GTAC 5 cut(s) 325, 454, 545, 775, 831
CviAII CATG 3 cut(s) 43, 205, 858
CviQI GTAC 5 cut(s) 325, 454, 545, 775, 831
DdeI CTNAG 1 cut(s) 171
DpnI GATC 3 cut(s) 71, 374, 849
DpnII GATC 3 cut(s) 69, 372, 847
DraI TTTAAA 1 cut(s) 307
DriI GACNNNNNGTC 1 cut(s) 498
EaeI YGGCCR 2 cut(s) 285, 725
Eam1104I CTCTTC 1 cut(s) 231
Eam1105I GACNNNNNGTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 807
Eco147I AGGCCT 1 cut(s) 598
Eco32I GATATC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 462
Eco81I CCTNAGG 1 cut(s) 171
EcoO109I RGGNCCY 1 cut(s) 175
EcoRII CCWGG 3 cut(s) 98, 456, 739
EcoRV GATATC 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 807
EcoT22I ATGCAT 1 cut(s) 859
ErhI CCWWGG 1 cut(s) 807
FaeI CATG 3 cut(s) 46, 208, 861
FaqI GGGAC 1 cut(s) 505
FatI CATG 3 cut(s) 42, 204, 857
FauI CCCGC 1 cut(s) 19
FauNDI CATATG 1 cut(s) 387
FblI GTMKAC 3 cut(s) 31, 209, 349
Fnu4HI GCNGC 4 cut(s) 49, 288, 317, 759
FokI GGATG 3 cut(s) 696, 735, 764
Fsp4HI GCNGC 4 cut(s) 49, 288, 317, 759
FspBI CTAG 3 cut(s) 338, 498, 862
GlaI GCGC 2 cut(s) 6, 514
GluI GCNGC 4 cut(s) 49, 288, 317, 759
GsuI CTGGAG 1 cut(s) 501
HaeIII GGCC 4 cut(s) 177, 287, 598, 727
HapII CCGG 1 cut(s) 334
HgaI GACGC 1 cut(s) 280
HhaI GCGC 2 cut(s) 7, 515
Hin1II CATG 3 cut(s) 46, 208, 861
Hin6I GCGC 2 cut(s) 5, 513
HinP1I GCGC 2 cut(s) 5, 513
HincII GTYRAC 3 cut(s) 32, 106, 210
HindII GTYRAC 3 cut(s) 32, 106, 210
HinfI GANTC 2 cut(s) 75, 211
HpaII CCGG 1 cut(s) 334
HphI GGTGA 3 cut(s) 181, 577, 653
Hpy166II GTNNAC 6 cut(s) 32, 106, 130, 210, 350, 454
Hpy188I TCNGA 4 cut(s) 74, 83, 252, 790
Hpy188III TCNNGA 5 cut(s) 91, 215, 380, 401, 628
Hpy8I GTNNAC 6 cut(s) 32, 106, 130, 210, 350, 454
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 6 cut(s) 97, 119, 410, 437, 442, 723
HpyCH4III ACNGT 3 cut(s) 127, 193, 779
HpyCH4IV ACGT 1 cut(s) 704
HpyCH4V TGCA 4 cut(s) 430, 467, 782, 857
HpyF10VI GCNNNNNNNGC 3 cut(s) 174, 243, 309
HpyF3I CTNAG 1 cut(s) 171
HpySE526I ACGT 1 cut(s) 704
Hsp92II CATG 3 cut(s) 46, 208, 861
HspAI GCGC 2 cut(s) 5, 513
Kzo9I GATC 3 cut(s) 69, 372, 847
LmnI GCTCC 2 cut(s) 382, 520
Lsp1109I GCAGC 1 cut(s) 770
LweI GCATC 5 cut(s) 246, 250, 454, 558, 758
MaeI CTAG 3 cut(s) 338, 498, 862
MaeII ACGT 1 cut(s) 704
MaeIII GTNAC 2 cut(s) 520, 705
MalI GATC 3 cut(s) 71, 374, 849
MbiI CCGCTC 1 cut(s) 162
MboI GATC 3 cut(s) 69, 372, 847
MboII GAAGA 6 cut(s) 21, 218, 305, 473, 489, 573
MfeI CAATTG 1 cut(s) 540
MflI RGATCY 1 cut(s) 372
MlsI TGGCCA 1 cut(s) 727
MluCI AATT 5 cut(s) 265, 540, 653, 795, 822
MluNI TGGCCA 1 cut(s) 727
MlyI GAGTC 2 cut(s) 84, 205
MmeI TCCRAC 1 cut(s) 61
MnlI CCTC 8 cut(s) 164, 188, 192, 234, 356, 545, 609, 748
Mox20I TGGCCA 1 cut(s) 727
Mph1103I ATGCAT 1 cut(s) 859
MscI TGGCCA 1 cut(s) 727
MseI TTAA 2 cut(s) 306, 615
MslI CAYNNNNRTG 2 cut(s) 390, 766
Msp20I TGGCCA 1 cut(s) 727
MspI CCGG 1 cut(s) 334
MspR9I CCNGG 3 cut(s) 100, 458, 741
MunI CAATTG 1 cut(s) 540
Mva1269I GAATGC 1 cut(s) 51
MvaI CCWGG 3 cut(s) 100, 458, 741
MvnI CGCG 4 cut(s) 7, 92, 274, 513
MwoI GCNNNNNNNGC 3 cut(s) 174, 243, 309
NdeI CATATG 1 cut(s) 387
NdeII GATC 3 cut(s) 69, 372, 847
NlaIII CATG 3 cut(s) 46, 208, 861
NlaIV GGNNCC 1 cut(s) 85
NmuCI GTSAC 1 cut(s) 705
NruI TCGCGA 1 cut(s) 92
NsiI ATGCAT 1 cut(s) 859
NspI RCATGY 2 cut(s) 208, 861
PaeI GCATGC 1 cut(s) 861
PaqCI CACCTGC 1 cut(s) 667
PceI AGGCCT 1 cut(s) 598
PciI ACATGT 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 320
PctI GAATGC 1 cut(s) 51
PkrI GCNGC 4 cut(s) 50, 289, 318, 760
PleI GAGTC 2 cut(s) 83, 205
PpsI GAGTC 2 cut(s) 83, 205
PscI ACATGT 1 cut(s) 204
PsiI TTATAA 1 cut(s) 840
Psp6I CCWGG 3 cut(s) 98, 456, 739
PspGI CCWGG 3 cut(s) 98, 456, 739
PspN4I GGNNCC 1 cut(s) 85
PspPI GGNCC 1 cut(s) 175
PsuI RGATCY 1 cut(s) 372
RruI TCGCGA 1 cut(s) 92
RsaI GTAC 5 cut(s) 326, 455, 546, 776, 832
RsaNI GTAC 5 cut(s) 325, 454, 545, 775, 831
RseI CAYNNNNRTG 2 cut(s) 390, 766
SalI GTCGAC 2 cut(s) 30, 208
SaqAI TTAA 2 cut(s) 306, 615
SatI GCNGC 4 cut(s) 49, 288, 317, 759
Sau3AI GATC 3 cut(s) 69, 372, 847
Sau96I GGNCC 1 cut(s) 175
SchI GAGTC 2 cut(s) 84, 205
ScrFI CCNGG 3 cut(s) 100, 458, 741
SfaNI GCATC 5 cut(s) 246, 250, 454, 558, 758
SmiMI CAYNNNNRTG 2 cut(s) 390, 766
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
SphI GCATGC 1 cut(s) 861
Sse9I AATT 5 cut(s) 265, 540, 653, 795, 822
SseBI AGGCCT 1 cut(s) 598
SsiI CCGC 6 cut(s) 26, 48, 160, 274, 288, 317
SspMI CTAG 3 cut(s) 338, 498, 862
StuI AGGCCT 1 cut(s) 598
StyD4I CCNGG 3 cut(s) 98, 456, 739
StyI CCWWGG 1 cut(s) 807
TaaI ACNGT 3 cut(s) 127, 193, 779
TaiI ACGT 1 cut(s) 707
TaqI TCGA 4 cut(s) 31, 209, 558, 850
TasI AATT 5 cut(s) 265, 540, 653, 795, 822
TatI WGTACW 2 cut(s) 544, 774
TauI GCSGC 3 cut(s) 51, 290, 319
Tru1I TTAA 2 cut(s) 306, 615
Tru9I TTAA 2 cut(s) 306, 615
TscAI CASTG 1 cut(s) 784
TseFI GTSAC 1 cut(s) 705
TseI GCWGC 1 cut(s) 758
Tsp45I GTSAC 1 cut(s) 705
TspDTI ATGAA 4 cut(s) 404, 408, 665, 686
TspRI CASTG 1 cut(s) 784
XapI RAATTY 3 cut(s) 653, 795, 822
XceI RCATGY 2 cut(s) 208, 861
XmiI GTMKAC 3 cut(s) 31, 209, 349
XspI CTAG 3 cut(s) 338, 498, 862
Zsp2I ATGCAT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.