Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0008496

Physical Location & Seq

Genomic Coordinates Reverse (-)

6288062 .. 6293924

Gene Structure 5863 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g11530.t6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3639 bp

ATGGGAGCTCTGGTGCTGAGGAAGTTGTGTGATAAAGTTTCTAGCTTTGCTATATTTCCATGGAGAAGAAGCAACACTAACAGTGCTGCTACAAGCCCCGAAGGCCAGGGGAGAAGAATCTTTCATAGAGATGTTGAAAGAGACCAGTTCCAGTATGCAAATGCTTACTGTCTTTCGTCATACTACAGTGTCTTTGTGGCTCGCCTTGCCATCATGGTTATGCTAGCTATTCTAATAGGGCTGTTAACTATACTGACATGGCATTTCACAAGAATATACACAGCAAAATCACTGAGCAACTTAGCTTATGGCCTTCGTTACGAGCTTCTGCAACGCCCCATTTTGCGGATGTGGAACATCTTGAATTCGACGGCAGAGATTACAACAGCGCAGGTGAAGCTTTCGGAGTATGTGATCAGAAAGTATAGCAAGCCTGCTACTCAAGCAGAACAAGTTGAGCTCTATGAATCGATGAGGGCTATAACGTGGGCAATGTTTGCTAGTCGAAAAGCTCTTAGTGCCATTACTATCAACTACAGAAATGGTTTTGTTCAGGCATTCCATAGAGATCACAGGAGTAACAACTCGTTTTATATCTACTCTGATCTTGCAAACTATTCAATCACTTCCACTGAGGCTTATGGTGTTGATATGTTCTCAACACGTGAGGCATGGAATGATCAGTCCATACATGGCAATACTTCTGCAAAGTGGTTCCGCGAACCACTCGATCCTGTCACAGGTGAGAAGATAGGGAAAGCAACCCAAATTCAGCCAGATGATCTGATCAACATAGCAGGCCTTTCACAAGTACCAGATGGTGTGGCCACTTGGCATGTGGCGGTAAGCAAGTATTCGGATTCACCATTGCTTTCAGCAGCACTGCCGGTTTCTGATCCTTCCAATAAAAGCATAGTGGCTGTGGTGGGTGTTACCACAGCACTGTATAGTGTTGGACAACTGATGAAAGAGCTTGTTGAGTTTCACAGTGGGCATATATATTTGACATCTCAAGAAGGTTACCTACTTTCTACTTCCACAAATGCTCCTCTTTTGACAAATACTACAAAAGGACCGAAGCTTATGATGGCTACAGATTCGGAGGATCACACAATAAGAATGGGATCTGAGTGGTTACAAAGAGCATATGGCAACAAGTTTCCTCCTAGGGATCAGGAGGTTCATGTTGAAAATGCCATGCTAGGACATGAGCAATATTACATCGACTCATTTTTTCTGAAGTTAAAGAGACTTCCTCTGGTAGGGGTTATCATCATTCCAAGAAAATATATAATGGGGAAGGTGGATGAGAGAGCCTTCAAAACGTTGGTGATCTTGATATCTGCATCATTTTGTATCCTAGTCACTGGATGTGTATGCATTTTCATACTGACAAATGGAGTATCAAAGGAAATGAAACTAAGGGCAGAGCTGATAAGTCATCTTGATGCGAGGAGACGGGCAGAGGCATCTAGCAACTATAAAAGCCAATTTCTAGCAAACATGAGTCATGAATTAAGGACACCAATGGCTGCAGTCATTGGATTACTGGACATTCTTATCAGCGATGATTGCCTTACAAATGAACAGTATTCCACAGTAACTCAGATTAGAAAATGCTCAACGGCTCTACTTCGGCTTCTTAACAATATACTGGATCTGAGCAAGGTTGAATCTGGAAAACTGGTGCTGGAAGAAGCTGAGTTTGACTTGGGAAGAGAACTTGAAGGTCTATTTGATATGTTCTCTGTGCAGTGCATCAACCACAATGTGGAGACTGTTTTAGATCTTTCTGATGATATGCCAAGATTAGTACAAGGAGACTCGGCAAGAGTTGTTCAAATTTTTGCTAATCTAATCAGCAATTCTATCAAGTTCACTACATCTGGTCACATAATACTTAGAGGATCGTGTGAGAACTTGGATGCTTCTGGTGATACCAGGAGAATCAGTCTCGATCAGAAAAGTTTACGGTCTTCACATAAGGCAAAGTTGAAGCAAGGGAACAATGCAAAGAAGAACCCCAAGAAAGATAACAAAATGGTTCTTTTGTTTGAAGTTGATGACACTGGTTGCGGAATTGATGCTAGTAAATGGGAGTCTGTCTTTGAAAGCTTTGAGCAAGCTGATCCCTCAACAACTCGAACGCATGGCGGCACTGGTCTTGGGCTATGCATTGTGCGGACAATGGTTAACAAGATGGGTGGAGATATCAAGGTTGTAAAGAAGGATGGGCCAGGAACTCTAATGCAGTTGTACTTGGTTCTGAATACACCTGCAGATGGCACGGAGCAATATTGTCAAGCAGATTTTTCAAAGCATAGTGTAGTGGTGCTGCTTGCACTACATGGCAGCATGGGCAGATTGATTATGTCCCGGTGGTTACGCAAAAATGGAGTGTTCACATTGGCAGCATCCGAGTGGAATGAACTGACACAAATGCTTAGAGAACTCTTTAAAGGCAGAAACTCAGATCATAGGAATATCTTTGAAACACAGCATTCTCTAAATGAATCCTTGAAAGGTGAAATTCGAAGTATACATGATATAAGGAACCCAGTTTTTGTCATGGTTATCGATATAGGAGTGCTCGACTTGAGCACAGATATATGGAAGGAACAGCTTAATTTCCTTGACAAATACTCTGGGAAAGCCACGTTTGCATGGATGCTGAATCATGATACCTCCAATGCCATAAAGATGGAGCTTCGCAGAAGAGGGAATGTATTGATGGTTAACAAGCCTTTATACAAAGCAAAAATGGCTCACATTTTGGAAGCTGTCATAAAAGAGAGAAATTTTGAAACACATGAATGCCTTGAGATTGATTCTACTCACTTTGATGTTGGCTGCAGCTCTGATGAATCTGATAAATGTGAAAAGAACAACTCCAGCTCCAAAAGTTCATTTCATATTGGTGAAAGACGAAGAGACAGGGTTGTGAAAAATTGTTCGTCACAGTACCAGACTATTAACAGCTGCTTAGTCGAACTGACAGAGGTATTCCCAAAGGACAGTAGTTTGAGAAAAGAAGATGTAGAGCCTAGACTAATGAGCACCAGTCAAGTATCCCATGCTACACAACCTCAATGTGAGGATTCAAAACTTAAAGAACAGCTATCAGTCACCAGGAGTCCTAGGGAGAATGGCAATGCATACACTAGGAAACCTGTGAATCAACCGAAGTCTCTTCAAGGCCTACGCATACTGCTTGCCGAAGACACACCAGTACTGCAGAGAGTTGCAAGTATAATGCTAGAAAAGATGGGAGCTACAGTAATTGCTGTGGCTGATGGACTGCAGGCAGTAGATGCTTTGAGTTGTATGCTCGATGCAGAAAATTGTAGGCAGGACATAAACGATGGACACGAAAATGAAAGTTTGGTTCCGCCCCCATATGACTTGGTTCTAATGGATTGTCAGATGCCAAAGATGGATGGTTATGAAGCAACAAAAGCAATCAGGAAATCCGAAGAGGGAACCAGTTTGCATATTCCAATCGTTGCTCTAACAGCTCATGCAATGTCATCAGATGAAGCAAAATGCTTGGAGGTGGGGATGGATGCTTATCTAACAAAGCCGATTGATTACAAGTTGATGGTGTCTACCATTCTCTCACTCACTAAAACAACAGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005034 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009791 GO:0009927 GO:0009987 GO:0010035 GO:0010154 GO:0010375 GO:0010431 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0021700 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0032504 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071695 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HATPase_c HisKA Response_reg

Protein Analysis

1213

Amino Acids

135.86

Weight (kDa)

7.17

Isoelectric Point (pI)

39.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HisKA	PF00512	355 - 420	1.4e-17	His Kinase A (phospho-acceptor) domain
HATPase_c	PF02518	467 - 615	5.6e-18	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg	PF00072	929 - 1063	7.8e-19	Response regulator receiver domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008496)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17820
fragaria_vesca	FvH4_1g11530 FvH4_1g11530 FvH4_1g11530 FvH4_1g11530 FvH4_1g11530 FvH4_1g11530
malus_domestica	MD02G1128300.v1.1 MD15G1243500.v1.1
prunus_persica	Prupe.7G170700_v2.0.a1 Prupe.7G170700_v2.0.a1
pyrus_communis	pycom02g10030
rosa_chinensis	RchiOBHm_Chr2g0099171
rosa_laevigata	RLG00000016852
rosa_multiflora	Rmu_sc0009113.1_g000004
rosa_roxburghii	Rroxscaffold_2G00143390
rosa_rugosa	Rorug02G0077300 Rorug02G0077400
rosa_samantha	Rh2AG126300 Rh2BG129500 Rh2CG130500 Rh2DG130900
rosa_wichuraiana	Rw2G009740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	382, 2284
Acc36I	ACCTGC	2 cut(s)	382, 2284
AccB7I	CCANNNNNTGG	1 cut(s)	1771
AccI	GTMKAC	2 cut(s)	2539, 3605
AccII	CGCG	1 cut(s)	720
AciI	CCGC	7 cut(s)	346, 718, 842, 2076, 2154, 2182, 3389
AclI	AACGTT	1 cut(s)	1325
AclWI	GGATC	8 cut(s)	725, 890, 1113, 1132, 1179, 1665, 1915, 2123
AcoI	YGGCCR	1 cut(s)	825
AcsI	RAATTY	5 cut(s)	364, 768, 1842, 2529, 2797
AcuI	CTGAAG	1 cut(s)	1259
AcvI	CACGTG	1 cut(s)	665
AdeI	CACNNNGTG	1 cut(s)	1771
AfaI	GTAC	5 cut(s)	813, 1815, 2258, 2963, 3231
AfiI	CCNNNNNNNGG	5 cut(s)	345, 740, 1262, 1771, 2282
AflIII	ACRYGT	1 cut(s)	662
AjnI	CCWGG	4 cut(s)	105, 1940, 2236, 3128
Alw21I	GWGCWC	5 cut(s)	10, 462, 2592, 2603, 3059
Alw26I	GTCTC	8 cut(s)	135, 1243, 1450, 1769, 1815, 1958, 2925, 3192
AlwI	GGATC	8 cut(s)	725, 890, 1113, 1132, 1179, 1665, 1915, 2123
AoxI	GGCC	6 cut(s)	103, 310, 799, 825, 2234, 3196
ApeKI	GCWGC	9 cut(s)	86, 878, 1532, 2335, 2352, 2411, 2850, 2853, 2979
ApoI	RAATTY	5 cut(s)	364, 768, 1842, 2529, 2797
AspA2I	CCTAGG	2 cut(s)	1166, 3137
AspLEI	GCGC	1 cut(s)	391
AspS9I	GGNCC	2 cut(s)	1073, 2234
AsuC2I	CCSGG	1 cut(s)	2377
AsuHPI	GGTGA	8 cut(s)	406, 755, 855, 1342, 1946, 2537, 2930, 3118
AsuII	TTCGAA	1 cut(s)	2533
AsuNHI	GCTAGC	1 cut(s)	223
AvaII	GGWCC	1 cut(s)	1073
AvrII	CCTAGG	2 cut(s)	1166, 3137
BalI	TGGCCA	1 cut(s)	827
BanII	GRGCYC	2 cut(s)	10, 462
BarI	GAAGNNNNNNTAC	2 cut(s)	1008, 1040
BbrPI	CACGTG	1 cut(s)	665
BbsI	GAAGAC	2 cut(s)	1968, 3225
Bbv12I	GWGCWC	5 cut(s)	10, 462, 2592, 2603, 3059
BbvCI	CCTCAGC	1 cut(s)	17
BbvI	GCAGC	9 cut(s)	73, 890, 1519, 2322, 2364, 2423, 2837, 2865, 2966
BceAI	ACGGC	2 cut(s)	387, 1641
BciT130I	CCWGG	4 cut(s)	107, 1942, 2238, 3130
BciVI	GTATCC	2 cut(s)	1367, 3079
BclI	TGATCA	3 cut(s)	414, 679, 786
BcnI	CCSGG	1 cut(s)	2377
BcoDI	GTCTC	8 cut(s)	135, 1243, 1450, 1769, 1815, 1958, 2925, 3192
BfmI	CTRYAG	9 cut(s)	184, 535, 1092, 1533, 2277, 2851, 3233, 3273, 3299
BfuAI	ACCTGC	2 cut(s)	382, 2284
BfuI	GTATCC	2 cut(s)	1367, 3079
BglI	GCCNNNNNGGC	1 cut(s)	102
BglII	AGATCT	1 cut(s)	1786
BlnI	CCTAGG	2 cut(s)	1166, 3137
BmcAI	AGTACT	1 cut(s)	3231
Bme1390I	CCNGG	5 cut(s)	107, 1942, 2238, 2377, 3130
Bme18I	GGWCC	1 cut(s)	1073
BmgT120I	GGNCC	2 cut(s)	1073, 2234
BmiI	GGNNCC	4 cut(s)	716, 2555, 3387, 3481
BmrFI	CCNGG	5 cut(s)	107, 1942, 2238, 2377, 3130
BmrI	ACTGGG	1 cut(s)	2552
BmtI	GCTAGC	1 cut(s)	227
BmuI	ACTGGG	1 cut(s)	2552
BpiI	GAAGAC	2 cut(s)	1968, 3225
BplI	GAGNNNNNCTC	2 cut(s)	1240, 1272
BpmI	CTGGAG	1 cut(s)	2875
Bpu10I	CCTNAGC	1 cut(s)	17
Bpu14I	TTCGAA	1 cut(s)	2533
BpuEI	CTTGAG	4 cut(s)	426, 996, 2617, 2840
BpuMI	CCSGG	1 cut(s)	2377
Bsa29I	ATCGAT	2 cut(s)	470, 2577
BsaAI	YACGTR	1 cut(s)	665
BsaBI	GATNNNNATC	1 cut(s)	3567
BsaI	GGTCTC	1 cut(s)	135
BsaJI	CCNNGG	4 cut(s)	59, 106, 1166, 3137
BsaXI	ACNNNNNCTCC	9 cut(s)	27, 1030, 1060, 1936, 1966, 2879, 2909, 3261, 3291
Bsc4I	CCNNNNNNNGG	5 cut(s)	345, 740, 1262, 1771, 2282
Bse118I	RCCGGY	1 cut(s)	886
Bse3DI	GCAATG	4 cut(s)	498, 866, 3157, 3528
Bse8I	GATNNNNATC	1 cut(s)	3567
BseBI	CCWGG	4 cut(s)	107, 1942, 2238, 3130
BseCI	ATCGAT	2 cut(s)	470, 2577
BseDI	CCNNGG	4 cut(s)	59, 106, 1166, 3137
BseJI	GATNNNNATC	1 cut(s)	3567
BseLI	CCNNNNNNNGG	5 cut(s)	345, 740, 1262, 1771, 2282
BseMI	GCAATG	4 cut(s)	498, 866, 3157, 3528
BseMII	CTCAG	8 cut(s)	8, 284, 624, 1119, 1619, 1652, 1692, 2484
BseRI	GAGGAG	2 cut(s)	1038, 1468
BseXI	GCAGC	9 cut(s)	73, 890, 1519, 2322, 2364, 2423, 2837, 2865, 2966
BsgI	GTGCAG	1 cut(s)	1772
Bsh1236I	CGCG	1 cut(s)	720
BshFI	GGCC	6 cut(s)	105, 312, 801, 827, 2236, 3198
BshVI	ATCGAT	2 cut(s)	470, 2577
BsiHKAI	GWGCWC	5 cut(s)	10, 462, 2592, 2603, 3059
BsiSI	CCGG	2 cut(s)	887, 2377
BslFI	GGGAC	1 cut(s)	2359
BslI	CCNNNNNNNGG	5 cut(s)	345, 740, 1262, 1771, 2282
BsmAI	GTCTC	8 cut(s)	135, 1243, 1450, 1769, 1815, 1958, 2925, 3192
BsmBI	CGTCTC	1 cut(s)	1450
BsmFI	GGGAC	1 cut(s)	2359
BsmI	GAATGC	3 cut(s)	557, 2500, 2819
BsnI	GGCC	6 cut(s)	105, 312, 801, 827, 2236, 3198
Bso31I	GGTCTC	1 cut(s)	135
Bsp119I	TTCGAA	1 cut(s)	2533
Bsp1286I	GDGCHC	5 cut(s)	10, 462, 2592, 2603, 3059
Bsp19I	CCATGG	1 cut(s)	59
BspACI	CCGC	7 cut(s)	346, 718, 842, 2076, 2154, 2182, 3389
BspANI	GGCC	6 cut(s)	105, 312, 801, 827, 2236, 3198
BspCNI	CTCAG	8 cut(s)	9, 285, 625, 1120, 1618, 1653, 1693, 2483
BspDI	ATCGAT	2 cut(s)	470, 2577
BspFNI	CGCG	1 cut(s)	720
BspHI	TCATGA	2 cut(s)	1510, 2677
BspLI	GGNNCC	4 cut(s)	716, 2555, 3387, 3481
BspMAI	CTGCAG	5 cut(s)	1537, 2281, 2855, 3237, 3303
BspMI	ACCTGC	2 cut(s)	382, 2284
BspOI	GCTAGC	1 cut(s)	227
BspPI	GGATC	8 cut(s)	725, 890, 1113, 1132, 1179, 1665, 1915, 2123
BspT104I	TTCGAA	1 cut(s)	2533
BspTNI	GGTCTC	1 cut(s)	135
BsrDI	GCAATG	4 cut(s)	498, 866, 3157, 3528
BsrFI	RCCGGY	1 cut(s)	886
BssAI	RCCGGY	1 cut(s)	886
BssECI	CCNNGG	4 cut(s)	59, 106, 1166, 3137
BssNAI	GTATAC	1 cut(s)	2540
BssT1I	CCWWGG	3 cut(s)	59, 1166, 3137
Bst1107I	GTATAC	1 cut(s)	2540
Bst2UI	CCWGG	4 cut(s)	107, 1942, 2238, 3130
Bst6I	CTCTTC	5 cut(s)	1711, 2710, 2923, 3195, 3468
BstAPI	GCANNNNNTGC	2 cut(s)	497, 3311
BstBAI	YACGTR	1 cut(s)	665
BstBI	TTCGAA	1 cut(s)	2533
BstC8I	GCNNGC	9 cut(s)	202, 225, 431, 435, 799, 2124, 2340, 3213, 3303
BstDSI	CCRYGG	1 cut(s)	59
BstEII	GGTNACC	1 cut(s)	1019
BstENI	CCTNNNNNAGG	2 cut(s)	738, 1260
BstFNI	CGCG	1 cut(s)	720
BstHHI	GCGC	1 cut(s)	391
BstMAI	GTCTC	8 cut(s)	135, 1243, 1450, 1769, 1815, 1958, 2925, 3192
BstNI	CCWGG	4 cut(s)	107, 1942, 2238, 3130
BstNSI	RCATGY	1 cut(s)	839
BstPI	GGTNACC	1 cut(s)	1019
BstSCI	CCNGG	5 cut(s)	105, 1940, 2236, 2375, 3128
BstSFI	CTRYAG	9 cut(s)	184, 535, 1092, 1533, 2277, 2851, 3233, 3273, 3299
BstUI	CGCG	1 cut(s)	720
BstV1I	GCAGC	9 cut(s)	73, 890, 1519, 2322, 2364, 2423, 2837, 2865, 2966
BstV2I	GAAGAC	2 cut(s)	1968, 3225
BstX2I	RGATCY	3 cut(s)	1124, 1657, 1786
BstXI	CCANNNNNNTGG	1 cut(s)	2701
BstYI	RGATCY	3 cut(s)	1124, 1657, 1786
BstZ17I	GTATAC	1 cut(s)	2540
Bsu15I	ATCGAT	2 cut(s)	470, 2577
BsuI	GTATCC	2 cut(s)	1367, 3079
BsuRI	GGCC	6 cut(s)	105, 312, 801, 827, 2236, 3198
BsuTUI	ATCGAT	2 cut(s)	470, 2577
BtgI	CCRYGG	1 cut(s)	59
BtgZI	GCGATG	1 cut(s)	1581
BtsI	GCAGTG	2 cut(s)	881, 1760
BveI	ACCTGC	2 cut(s)	382, 2284
Cac8I	GCNNGC	9 cut(s)	202, 225, 431, 435, 799, 2124, 2340, 3213, 3303
CciI	TCATGA	2 cut(s)	1510, 2677
CfoI	GCGC	1 cut(s)	391
Cfr10I	RCCGGY	1 cut(s)	886
Cfr13I	GGNCC	2 cut(s)	1073, 2234
ClaI	ATCGAT	2 cut(s)	470, 2577
Csp6I	GTAC	5 cut(s)	812, 1814, 2257, 2962, 3230
CspCI	CAANNNNNGTGG	2 cut(s)	2185, 2220
CviQI	GTAC	5 cut(s)	812, 1814, 2257, 2962, 3230
DraI	TTTAAA	1 cut(s)	2458
DraIII	CACNNNGTG	1 cut(s)	1771
EaeI	YGGCCR	1 cut(s)	825
Eam1104I	CTCTTC	5 cut(s)	1711, 2710, 2923, 3195, 3468
EarI	CTCTTC	5 cut(s)	1711, 2710, 2923, 3195, 3468
EciI	GGCGGA	1 cut(s)	3378
Ecl136II	GAGCTC	2 cut(s)	8, 460
Eco130I	CCWWGG	3 cut(s)	59, 1166, 3137
Eco147I	AGGCCT	2 cut(s)	801, 3198
Eco24I	GRGCYC	2 cut(s)	10, 462
Eco31I	GGTCTC	1 cut(s)	135
Eco32I	GATATC	2 cut(s)	1341, 2212
Eco47I	GGWCC	1 cut(s)	1073
Eco53kI	GAGCTC	2 cut(s)	8, 460
Eco57I	CTGAAG	1 cut(s)	1259
Eco72I	CACGTG	1 cut(s)	665
Eco91I	GGTNACC	1 cut(s)	1019
EcoICRI	GAGCTC	2 cut(s)	8, 460
EcoNI	CCTNNNNNAGG	2 cut(s)	738, 1260
EcoO65I	GGTNACC	1 cut(s)	1019
EcoRI	GAATTC	1 cut(s)	364
EcoRII	CCWGG	4 cut(s)	105, 1940, 2236, 3128
EcoRV	GATATC	2 cut(s)	1341, 2212
EcoT14I	CCWWGG	3 cut(s)	59, 1166, 3137
EcoT22I	ATGCAT	3 cut(s)	1382, 2177, 3157
EcoT38I	GRGCYC	2 cut(s)	10, 462
ErhI	CCWWGG	3 cut(s)	59, 1166, 3137
Esp3I	CGTCTC	1 cut(s)	1450
FaqI	GGGAC	1 cut(s)	2359
FauNDI	CATATG	2 cut(s)	1147, 3397
FbaI	TGATCA	3 cut(s)	414, 679, 786
FblI	GTMKAC	2 cut(s)	2539, 3605
FriOI	GRGCYC	2 cut(s)	10, 462
GlaI	GCGC	1 cut(s)	390
GsuI	CTGGAG	1 cut(s)	2875
HaeIII	GGCC	6 cut(s)	105, 312, 801, 827, 2236, 3198
HapII	CCGG	2 cut(s)	887, 2377
HhaI	GCGC	1 cut(s)	391
Hin6I	GCGC	1 cut(s)	389
HinP1I	GCGC	1 cut(s)	389
HincII	GTYRAC	3 cut(s)	246, 2194, 2737
HindII	GTYRAC	3 cut(s)	246, 2194, 2737
HindIII	AAGCTT	3 cut(s)	398, 1079, 2113
HpaI	GTTAAC	3 cut(s)	246, 2194, 2737
HpaII	CCGG	2 cut(s)	887, 2377
HphI	GGTGA	8 cut(s)	406, 755, 855, 1342, 1946, 2537, 2930, 3118
Hpy166II	GTNNAC	8 cut(s)	246, 1878, 1970, 2194, 2403, 2540, 2737, 3606
Hpy8I	GTNNAC	8 cut(s)	246, 1878, 1970, 2194, 2403, 2540, 2737, 3606
Hpy99I	CGWCG	1 cut(s)	373
HpyAV	CCTTC	9 cut(s)	95, 323, 909, 1010, 1294, 1327, 1721, 2221, 2608
HpyCH4IV	ACGT	4 cut(s)	485, 664, 1325, 2657
HpySE526I	ACGT	4 cut(s)	485, 664, 1325, 2657
HspAI	GCGC	1 cut(s)	389
Ksp22I	TGATCA	3 cut(s)	414, 679, 786
KspAI	GTTAAC	3 cut(s)	246, 2194, 2737
LmnI	GCTCC	6 cut(s)	5, 1051, 2290, 2704, 2900, 3269
Lsp1109I	GCAGC	9 cut(s)	73, 890, 1519, 2322, 2364, 2423, 2837, 2865, 2966
MaeII	ACGT	4 cut(s)	485, 664, 1325, 2657
MflI	RGATCY	3 cut(s)	1124, 1657, 1786
MhlI	GDGCHC	5 cut(s)	10, 462, 2592, 2603, 3059
MlsI	TGGCCA	1 cut(s)	827
MluNI	TGGCCA	1 cut(s)	827
MlyI	GAGTC	5 cut(s)	1220, 1516, 1817, 2108, 3142
MmeI	TCCRAC	1 cut(s)	934
Mox20I	TGGCCA	1 cut(s)	827
Mph1103I	ATGCAT	3 cut(s)	1382, 2177, 3157
MscI	TGGCCA	1 cut(s)	827
MslI	CAYNNNNRTG	9 cut(s)	129, 218, 1446, 2419, 2699, 2812, 2841, 2916, 3372
Msp20I	TGGCCA	1 cut(s)	827
MspA1I	CMGCKG	1 cut(s)	2979
MspI	CCGG	2 cut(s)	887, 2377
MspR9I	CCNGG	5 cut(s)	107, 1942, 2238, 2377, 3130
Mva1269I	GAATGC	3 cut(s)	557, 2500, 2819
MvaI	CCWGG	4 cut(s)	107, 1942, 2238, 3130
MvnI	CGCG	1 cut(s)	720
NciI	CCSGG	1 cut(s)	2377
NcoI	CCATGG	1 cut(s)	59
NdeI	CATATG	2 cut(s)	1147, 3397
NheI	GCTAGC	1 cut(s)	223
NlaIV	GGNNCC	4 cut(s)	716, 2555, 3387, 3481
NmeAIII	GCCGAG	1 cut(s)	1805
NmuCI	GTSAC	5 cut(s)	736, 1363, 1889, 2955, 3124
NsiI	ATGCAT	3 cut(s)	1382, 2177, 3157
NspI	RCATGY	1 cut(s)	839
NspV	TTCGAA	1 cut(s)	2533
PagI	TCATGA	2 cut(s)	1510, 2677
PaqCI	CACCTGC	2 cut(s)	382, 2284
PceI	AGGCCT	2 cut(s)	801, 3198
PcsI	WCGNNNNNNNCGW	1 cut(s)	3366
PctI	GAATGC	3 cut(s)	557, 2500, 2819
PflMI	CCANNNNNTGG	1 cut(s)	1771
PleI	GAGTC	5 cut(s)	1220, 1515, 1817, 2107, 3141
PmaCI	CACGTG	1 cut(s)	665
PmlI	CACGTG	1 cut(s)	665
PpsI	GAGTC	5 cut(s)	1220, 1515, 1817, 2107, 3141
Ppu21I	YACGTR	1 cut(s)	665
Psp124BI	GAGCTC	2 cut(s)	10, 462
Psp1406I	AACGTT	1 cut(s)	1325
Psp6I	CCWGG	4 cut(s)	105, 1940, 2236, 3128
PspCI	CACGTG	1 cut(s)	665
PspEI	GGTNACC	1 cut(s)	1019
PspGI	CCWGG	4 cut(s)	105, 1940, 2236, 3128
PspN4I	GGNNCC	4 cut(s)	716, 2555, 3387, 3481
PspPI	GGNCC	2 cut(s)	1073, 2234
PstI	CTGCAG	5 cut(s)	1537, 2281, 2855, 3237, 3303
PsuI	RGATCY	3 cut(s)	1124, 1657, 1786
PvuII	CAGCTG	1 cut(s)	2979
RsaI	GTAC	5 cut(s)	813, 1815, 2258, 2963, 3231
RsaNI	GTAC	5 cut(s)	812, 1814, 2257, 2962, 3230
RseI	CAYNNNNRTG	9 cut(s)	129, 218, 1446, 2419, 2699, 2812, 2841, 2916, 3372
SacI	GAGCTC	2 cut(s)	10, 462
Sau96I	GGNCC	2 cut(s)	1073, 2234
ScaI	AGTACT	1 cut(s)	3231
SchI	GAGTC	5 cut(s)	1220, 1516, 1817, 2108, 3142
ScrFI	CCNGG	5 cut(s)	107, 1942, 2238, 2377, 3130
SduI	GDGCHC	5 cut(s)	10, 462, 2592, 2603, 3059
SfcI	CTRYAG	9 cut(s)	184, 535, 1092, 1533, 2277, 2851, 3233, 3273, 3299
SfuI	TTCGAA	1 cut(s)	2533
SinI	GGWCC	1 cut(s)	1073
SmiMI	CAYNNNNRTG	9 cut(s)	129, 218, 1446, 2419, 2699, 2812, 2841, 2916, 3372
SmlI	CTYRAG	4 cut(s)	441, 1011, 2596, 2819
SmoI	CTYRAG	4 cut(s)	441, 1011, 2596, 2819
SseBI	AGGCCT	2 cut(s)	801, 3198
SsiI	CCGC	7 cut(s)	346, 718, 842, 2076, 2154, 2182, 3389
SspI	AATATT	2 cut(s)	1217, 2297
SstI	GAGCTC	2 cut(s)	10, 462
StuI	AGGCCT	2 cut(s)	801, 3198
StyD4I	CCNGG	5 cut(s)	105, 1940, 2236, 2375, 3128
StyI	CCWWGG	3 cut(s)	59, 1166, 3137
TaiI	ACGT	4 cut(s)	488, 667, 1328, 2660
TaqII	GACCGA	1 cut(s)	1090
TatI	WGTACW	3 cut(s)	1813, 2256, 3229
TauI	GCSGC	1 cut(s)	2157
TseFI	GTSAC	5 cut(s)	736, 1363, 1889, 2955, 3124
TseI	GCWGC	9 cut(s)	86, 878, 1532, 2335, 2352, 2411, 2850, 2853, 2979
Tsp45I	GTSAC	5 cut(s)	736, 1363, 1889, 2955, 3124
TspGWI	ACGGA	1 cut(s)	2303
Van91I	CCANNNNNTGG	1 cut(s)	1771
VpaK11BI	GGWCC	1 cut(s)	1073
XagI	CCTNNNNNAGG	2 cut(s)	738, 1260
XapI	RAATTY	5 cut(s)	364, 768, 1842, 2529, 2797
XceI	RCATGY	1 cut(s)	839
XcmI	CCANNNNNNNNNTGG	1 cut(s)	835
XmaJI	CCTAGG	2 cut(s)	1166, 3137
XmiI	GTMKAC	2 cut(s)	2539, 3605
ZrmI	AGTACT	1 cut(s)	3231
Zsp2I	ATGCAT	3 cut(s)	1382, 2177, 3157

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.