Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0008496

Physical Location & Seq

Genomic Coordinates Reverse (-)

10371196 .. 10377110

Gene Structure 5915 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1128300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3690 bp

ATGGCAACTCCTCTAGGAAAAGTGTTTGATAAAATTTCAAGTTTTGCCACACCTTGGAGAAGGAGCAACACTACAACTCCTCAGGGAAGAAGAATCTTTCATAGGGATGTTGAAAGAGATCAGTTCCAGTATGGAAATGGTCATTGCCTCTCCTCCTACTACAGCGTCTTCGTCGCCCGCCTCGCCATCATGGTTATGCTAGCAATTTTAATAGGGCTACTAACCATACTGACATGGCATTTCACAAGGATCTACACTGCAAAATCGCTGAGCAACTTAGCATACGGGCTTCGCTATGAACTTCTGCAACGTCCTGTGTTAAGGATGTGGAACATCTTAAATTCCACGGCAGAAATAACAGCAGCGCAGGTTAAGCTCTCGGAGTATGTGATCAAACGGAACAGCAACCCTACCAGTCAAGCAGAACAAGTGGAGCTATATGAATCAATGAGGGCTGTGACGTGGGCAATGTTCGCGAGCCGTAAGGCTCTCAATGCAATCACTATAAACTACAGAAATGGTTTTGTCCAGGCATTCCACAGAGATCACAGGAGCAACAATACATACTACATCTACTCTGATCTCGCAAACTATTCGATCAGCGCAAGTGGTGCGTATGATGCTAATGCACTGTCAACACATCAGGCGTGGAGTGATCACTCCATACACGGCAACATTTCTGCAAAATGGTACCGCGAGCCACTTGATCCCGTCACAGGGGAGAAGACAGGGAAAGCAACCCAAATTCAACCGGATGATCTGATCAACATTGCAGGGCTTTCACAAGTACCGGATGGTGTGGCCACTTGGCACGTGGGGGTGAGCAAGTATTCGGATTCACCACTTCTTTCGGCTGCACTGGCAGTTTCCGACCCTTCAAATAAAAGAGTAGTGGCGGTTGTGGGTGTTACTACAGCTCTGTACAGTGTAGGGCAACTGATGAAAGAGCTTGTTGAGTTTCACAGTGGACATATTTACTTAACTTCTCAAGAAGGTTACTTGCTTGCTACGTCAACAAATGCTCCACTTTTGAGGAATTCGACGAGAGGGCCTAAGCTCATGATGGCTGTCGATTCTGAGGATCGCATAATACACTCAGGAGCAGAATGGTTGCAGCGAGCCTATGGTAACAAGTTTCCTCCTAGTCACGAGGTTCATGTTGAGAATGCCAGGCTTGGAAACCAGCAGTATTACATCGACTCGTTTTTTCTAAATTTAAAGAGACTTCCTCTGGTAGGGGTTATCATCATTCCGCGAAAATATATAATGGGGAAGGTAGACGAAAGAGCGTTCAAAACGTTGGTGATACTGATATCTGCATCCTTTTGCATTCTGGTGATTGGATGTGTTTGCATTTTCATACTCACGAATGGAGTTTCAAAGGAGATGAAGCTGAGGGCAGAACTGATAAGTCACCTTGATGCAAGAAGAAGGGCTGAGGCCTCTAGTAACTACAAAAGCCAATTTCTAGCCAATATGAGTCATGAACTGAGGACACCAATGGCGGCGGTGATTGGGCTGCTGGACATTATGATCAGTGATGATTGCCTTACAAATGAACAATACTCCACAGTTACTCAGATCAGAAAATGCTCGACAGCTCTACTTCGGCTTCTCAACAACATATTGGATCTCAGCAAGGTTGAATCTGGAAAAATGGTGTTGGAAGAAGCCGAGTTTGATCTGGGAAGAGAACTCGAGGGGCTCTTCGACATGTTCTCCGTGCAGTGCATTAACCACAATGTGGAGACTGTTTTAGATCTCTCTGATGATATTCCGAAATTAGTTCGAGGGGACTCTGCAAGAGTTGTTCAAATATTTGCTAATCTAATCAGCAACTCTATCAAGTTCACTACATCGGGTCACGTTATACTTAGAGGATCATGCGAGAACTTCAACATTTCTGGTGACACCAGGAGAATCCTTCTTGATCAGAAAAATTTGTGGCCGTCTCATAAGGCAAAGCTGAAGCAAAGCAACCATGTAAAAAAGGCTTCCAACAAGGACGACAACAAAATGATTCTTTGGTTCGAAGTTGATGACACTGGTTGCGGAATCGATCCGAGTAAATGGGAGTCTGTTTTTGAAAGCTTTGAGCAAGCTGATCCCTCAACAACTCGAACGCATGGGGGAACTGGACTTGGACTTTGCATTGTACGAACATTGGTTAACAAGATGGGTGGAGAAATCAAGGTTGTAAAGAAGGACGGGCAGGGTACCTTAATGCAATTTTACTTGGTTCTCAATACGCCTGCAGATGGCACGGAGCAACATTGTCAAGTAGATTTTGCAAAGAAAAATGTAGTGGTGCTGCTTGCACTACACGGCAGCATGGGGAGGTTGATTATGTCCCAGTGGTTGCGAAAAAAAGGAGTGTTCACTCTGGCAACATCTGAGTGGAATGAACTGACACAAATTCTTCGAGAACTCTTTCATAACAGGAATTCATCTCATAAAAACAGTTTCGAAAAACAGTATTCGCTGAGTGAATCTTTGAGAGGTGAAATACGAAATACACGTGACATGAGGAACCCAGCTTTCGTCATGGTTGTCGATATAGGGCTGCTTGACCTGAGCACAGATATATGGAAGGAGCAGCTTAACTTCCTTGATAAATACTCCGGGAAAGCAAACTTTGCATGGATGCTGAATCATGATACATCCAACGCTGTCAAGATGGAGCTCCGAAGAAAAGGGTATGTTTTGATGGTTAATAAACCGATGTACAAGGCGAAAATGGTTCATATTTTGGAAACTGTCATGACAGAGAGAGATCTTGACACGCAGAGGAGATCCGCAACCAGTTTGACAAACAGCACAAAAGAAGGTGACTTGCACGAATGCCTTGAGATCGATTCTACTCATTTTTATGTTCCCAGTAGCTCTGATGATTCTGATATATCTGAAAAGAACGACTCTAATTCTGAAAACACATCTCATGTCGAAGAAAAGCAAAGAGACGGGATCATGAAACCTAGTTCCCCGCAGTACCAGGCGGTTAACAGCTGCTTAGTTGAACTCACAAGCGTATGCTCGAAAGACAATAATTCAAGGAAAGAAGACACAGAAGATACGGAACATAAAGCAATGAGCAACAATCAAGCATCCCATGCAACAAAATCACAATGTGAAAACTCGGAAGTTCAAGAACATCGTTCGATCACCAGCAGTCCTAAAGAGAATGGTTGTTCATATAAAAGTAGAACTACAAATCAACAGAAATCTCTCGAAGGCGTGCGCATTCTACTCGCAGAAGATACACCGGTACTGCAGAGAGTTGCAACTATAATGCTCGAAAAAATGGGGGCTACTGTGATTGCTGTGGCTGATGGACTGCAGGCAGTAGATGCTCTCAACTGTGTGAACAAATCAGAAGATTGTAGAAAGGAATTTCACTCGAAAGATGGAGATACGAACTTGGATAATCAAACATGGGGTTTCCGTCCATTCGACTTGATTCTAATGGACTGTCAGATGCCGAAGATGGACGGTTATGAAGCAACGAAGGCAATCAGGAGATCGGAGGAGGGGACCGATTCGCACATTCCGATCGTTGCCCTAACAGCTCATGCAATGTCATCAGATGAAGCAAAGTGCTTGGAGGTGGGGATGGATGCTTATCTGACAAAGCCAATTGACTACAAGTTGATGGTGTCTACCATCCTTTCACTCACTAGAACAACAACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005034 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009791 GO:0009927 GO:0009987 GO:0010035 GO:0010154 GO:0010375 GO:0010431 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0021700 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0032504 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071695 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HATPase_c HisKA Response_reg

Protein Analysis

1230

Amino Acids

138.02

Weight (kDa)

6.96

Isoelectric Point (pI)

39.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HisKA	PF00512	486 - 551	4.8e-17	His Kinase A (phospho-acceptor) domain
HATPase_c	PF02518	598 - 747	1.1e-19	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg	PF00072	1144 - 1220	2.1e-17	Response regulator receiver domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008496)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17820
fragaria_vesca	FvH4_1g11530 FvH4_1g11530 FvH4_1g11530 FvH4_1g11530 FvH4_1g11530 FvH4_1g11530
malus_domestica	MD02G1128300.v1.1 MD15G1243500.v1.1
prunus_persica	Prupe.7G170700_v2.0.a1 Prupe.7G170700_v2.0.a1
pyrus_communis	pycom02g10030
rosa_chinensis	RchiOBHm_Chr2g0099171
rosa_laevigata	RLG00000016852
rosa_multiflora	Rmu_sc0009113.1_g000004
rosa_roxburghii	Rroxscaffold_2G00143390
rosa_rugosa	Rorug02G0077300 Rorug02G0077400
rosa_samantha	Rh2AG126300 Rh2BG129500 Rh2CG130500 Rh2DG130900
rosa_wichuraiana	Rw2G009740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	3239
Acc36I	ACCTGC	1 cut(s)	358
Acc65I	GGTACC	2 cut(s)	690, 2216
AccB1I	GGYRCC	2 cut(s)	690, 2216
AccB7I	CCANNNNNTGG	2 cut(s)	54, 1744
AccI	GTMKAC	2 cut(s)	1278, 3656
AccII	CGCG	3 cut(s)	476, 696, 1255
AclI	AACGTT	1 cut(s)	1298
AclWI	GGATC	9 cut(s)	257, 701, 1089, 1638, 1888, 2054, 2099, 2787, 2972
AcoI	YGGCCR	2 cut(s)	801, 1946
AcsI	RAATTY	9 cut(s)	33, 340, 744, 1036, 1213, 1939, 2415, 2443, 3389
AcuI	CTGAAG	1 cut(s)	1988
AcvI	CACGTG	2 cut(s)	814, 2519
AdeI	CACNNNGTG	2 cut(s)	1744, 3128
AfaI	GTAC	8 cut(s)	692, 789, 923, 2157, 2218, 2726, 2990, 3267
AfiI	CCNNNNNNNGG	6 cut(s)	54, 716, 717, 1235, 1744, 2258
AflIII	ACRYGT	2 cut(s)	1713, 2516
AgeI	ACCGGT	1 cut(s)	3262
AjiI	CACGTC	1 cut(s)	462
AjnI	CCWGG	4 cut(s)	528, 1169, 1913, 2991
Alw21I	GWGCWC	2 cut(s)	2579, 2685
Alw26I	GTCTC	4 cut(s)	1216, 1742, 1956, 2952
AlwI	GGATC	9 cut(s)	257, 701, 1089, 1638, 1888, 2054, 2099, 2787, 2972
Ama87I	CYCGRG	1 cut(s)	1697
AoxI	GGCC	4 cut(s)	801, 1049, 1440, 1946
ApeKI	GCWGC	9 cut(s)	362, 854, 1114, 1519, 2311, 2328, 2563, 2596, 3006
ApoI	RAATTY	9 cut(s)	33, 340, 744, 1036, 1213, 1939, 2415, 2443, 3389
ArsI	GACNNNNNNTTYG	2 cut(s)	2130, 2162
AsiGI	ACCGGT	1 cut(s)	3262
Asp700I	GAANNNNTTC	1 cut(s)	2430
Asp718I	GGTACC	2 cut(s)	690, 2216
AspLEI	GCGC	3 cut(s)	367, 605, 3240
AspS9I	GGNCC	2 cut(s)	1049, 3531
AsuC2I	CCSGG	1 cut(s)	2623
AsuII	TTCGAA	2 cut(s)	2031, 2466
AsuNHI	GCTAGC	1 cut(s)	199
AvaI	CYCGRG	1 cut(s)	1697
AvaII	GGWCC	1 cut(s)	3531
AxyI	CCTNAGG	1 cut(s)	81
BaeI	ACNNNNGTAYC	2 cut(s)	3249, 3282
BalI	TGGCCA	1 cut(s)	803
BanI	GGYRCC	2 cut(s)	690, 2216
BanII	GRGCYC	2 cut(s)	1707, 2685
BauI	CACGAG	1 cut(s)	1148
BbrPI	CACGTG	2 cut(s)	814, 2519
BbsI	GAAGAC	3 cut(s)	160, 731, 3066
Bbv12I	GWGCWC	2 cut(s)	2579, 2685
BbvCI	CCTCAGC	2 cut(s)	1394, 1437
BbvI	GCAGC	9 cut(s)	374, 841, 1126, 1506, 2298, 2340, 2550, 2608, 2993
BceAI	ACGGC	5 cut(s)	363, 465, 685, 1933, 2341
BcgI	CGANNNNNNTGC	4 cut(s)	576, 610, 3229, 3263
BciT130I	CCWGG	4 cut(s)	530, 1171, 1915, 2993
BclI	TGATCA	5 cut(s)	390, 655, 762, 1533, 1930
BcnI	CCSGG	1 cut(s)	2623
BcoDI	GTCTC	4 cut(s)	1216, 1742, 1956, 2952
BfaI	CTAG	7 cut(s)	14, 200, 1143, 1446, 1469, 2976, 3675
BfmI	CTRYAG	6 cut(s)	160, 511, 912, 2253, 3269, 3335
BfuAI	ACCTGC	1 cut(s)	358
BglII	AGATCT	2 cut(s)	1759, 2773
BlpI	GCTNAGC	1 cut(s)	269
Bme1390I	CCNGG	5 cut(s)	530, 1171, 1915, 2623, 2993
Bme18I	GGWCC	1 cut(s)	3531
BmeT110I	CYCGRG	1 cut(s)	1697
BmgBI	CACGTC	1 cut(s)	462
BmgT120I	GGNCC	2 cut(s)	1049, 3531
BmiI	GGNNCC	4 cut(s)	692, 2218, 2531, 3532
BmrFI	CCNGG	5 cut(s)	530, 1171, 1915, 2623, 2993
BmrI	ACTGGG	2 cut(s)	2347, 2871
BmsI	GCATC	8 cut(s)	610, 1328, 1411, 2634, 3113, 3337, 3465, 3604
BmtI	GCTAGC	1 cut(s)	203
BmuI	ACTGGG	2 cut(s)	2347, 2871
BpiI	GAAGAC	3 cut(s)	160, 731, 3066
BplI	GAGNNNNNCTC	2 cut(s)	1213, 1245
Bpu10I	CCTNAGC	4 cut(s)	1053, 1394, 1437, 2573
Bpu1102I	GCTNAGC	1 cut(s)	269
Bpu14I	TTCGAA	2 cut(s)	2031, 2466
BpuEI	CTTGAG	2 cut(s)	972, 2867
BpuMI	CCSGG	1 cut(s)	2623
Bsa29I	ATCGAT	2 cut(s)	2058, 2853
BsaAI	YACGTR	2 cut(s)	814, 2519
BsaBI	GATNNNNATC	1 cut(s)	3618
BsaJI	CCNNGG	2 cut(s)	53, 345
BsaWI	WCCGGW	3 cut(s)	751, 790, 3262
BsaXI	ACNNNNNCTCC	6 cut(s)	61, 91, 1006, 1036, 1703, 1733
Bsc4I	CCNNNNNNNGG	6 cut(s)	54, 716, 717, 1235, 1744, 2258
Bse118I	RCCGGY	1 cut(s)	3262
Bse1I	ACTGG	8 cut(s)	127, 414, 864, 2050, 2140, 2353, 2802, 2877
Bse21I	CCTNAGG	1 cut(s)	81
Bse3DI	GCAATG	5 cut(s)	142, 474, 768, 3093, 3579
Bse8I	GATNNNNATC	1 cut(s)	3618
BseBI	CCWGG	4 cut(s)	530, 1171, 1915, 2993
BseCI	ATCGAT	2 cut(s)	2058, 2853
BseDI	CCNNGG	2 cut(s)	53, 345
BseJI	GATNNNNATC	1 cut(s)	3618
BseLI	CCNNNNNNNGG	6 cut(s)	54, 716, 717, 1235, 1744, 2258
BseMI	GCAATG	5 cut(s)	142, 474, 768, 3093, 3579
BseNI	ACTGG	8 cut(s)	127, 414, 864, 2050, 2140, 2353, 2802, 2877
BseRI	GAGGAG	4 cut(s)	69, 142, 2803, 3539
BseXI	GCAGC	9 cut(s)	374, 841, 1126, 1506, 2298, 2340, 2550, 2608, 2993
BseYI	CCCAGC	1 cut(s)	2533
BsgI	GTGCAG	2 cut(s)	840, 1745
Bsh1236I	CGCG	3 cut(s)	476, 696, 1255
Bsh1285I	CGRYCG	1 cut(s)	3552
BshFI	GGCC	4 cut(s)	803, 1051, 1442, 1948
BshNI	GGYRCC	2 cut(s)	690, 2216
BshTI	ACCGGT	1 cut(s)	3262
BshVI	ATCGAT	2 cut(s)	2058, 2853
BsiEI	CGRYCG	1 cut(s)	3552
BsiHKAI	GWGCWC	2 cut(s)	2579, 2685
BsiHKCI	CYCGRG	1 cut(s)	1697
BsiSI	CCGG	4 cut(s)	752, 791, 2622, 3263
BslFI	GGGAC	3 cut(s)	1808, 2335, 3544
BslI	CCNNNNNNNGG	6 cut(s)	54, 716, 717, 1235, 1744, 2258
BsmAI	GTCTC	4 cut(s)	1216, 1742, 1956, 2952
BsmBI	CGTCTC	2 cut(s)	1956, 2952
BsmFI	GGGAC	3 cut(s)	1808, 2335, 3544
BsmI	GAATGC	5 cut(s)	533, 1171, 1329, 2846, 3240
BsnI	GGCC	4 cut(s)	803, 1051, 1442, 1948
BsoBI	CYCGRG	1 cut(s)	1697
Bsp119I	TTCGAA	2 cut(s)	2031, 2466
Bsp1286I	GDGCHC	3 cut(s)	1707, 2579, 2685
Bsp1407I	TGTACA	2 cut(s)	921, 2724
Bsp1720I	GCTNAGC	1 cut(s)	269
Bsp68I	TCGCGA	1 cut(s)	476
BspANI	GGCC	4 cut(s)	803, 1051, 1442, 1948
BspDI	ATCGAT	2 cut(s)	2058, 2853
BspFNI	CGCG	3 cut(s)	476, 696, 1255
BspHI	TCATGA	5 cut(s)	1059, 1483, 2653, 2760, 2967
BspLI	GGNNCC	4 cut(s)	692, 2218, 2531, 3532
BspMAI	CTGCAG	3 cut(s)	2257, 3273, 3339
BspMI	ACCTGC	1 cut(s)	358
BspOI	GCTAGC	1 cut(s)	203
BspPI	GGATC	9 cut(s)	257, 701, 1089, 1638, 1888, 2054, 2099, 2787, 2972
BspQI	GCTCTTC	1 cut(s)	1712
BspT104I	TTCGAA	2 cut(s)	2031, 2466
BspT107I	GGYRCC	2 cut(s)	690, 2216
BsrDI	GCAATG	5 cut(s)	142, 474, 768, 3093, 3579
BsrFI	RCCGGY	1 cut(s)	3262
BsrGI	TGTACA	2 cut(s)	921, 2724
BsrI	ACTGG	8 cut(s)	127, 414, 864, 2050, 2140, 2353, 2802, 2877
BssAI	RCCGGY	1 cut(s)	3262
BssECI	CCNNGG	2 cut(s)	53, 345
BssSI	CACGAG	1 cut(s)	1148
BssT1I	CCWWGG	1 cut(s)	53
Bst2BI	CACGAG	1 cut(s)	1148
Bst2UI	CCWGG	4 cut(s)	530, 1171, 1915, 2993
Bst6I	CTCTTC	2 cut(s)	1684, 1712
BstAPI	GCANNNNNTGC	4 cut(s)	611, 2636, 3110, 3347
BstAUI	TGTACA	2 cut(s)	921, 2724
BstBAI	YACGTR	2 cut(s)	814, 2519
BstBI	TTCGAA	2 cut(s)	2031, 2466
BstDSI	CCRYGG	1 cut(s)	345
BstENI	CCTNNNNNAGG	1 cut(s)	1233
BstFNI	CGCG	3 cut(s)	476, 696, 1255
BstHHI	GCGC	3 cut(s)	367, 605, 3240
BstMAI	GTCTC	4 cut(s)	1216, 1742, 1956, 2952
BstMCI	CGRYCG	1 cut(s)	3552
BstNI	CCWGG	4 cut(s)	530, 1171, 1915, 2993
BstNSI	RCATGY	1 cut(s)	1717
BstSCI	CCNGG	5 cut(s)	528, 1169, 1913, 2621, 2991
BstSFI	CTRYAG	6 cut(s)	160, 511, 912, 2253, 3269, 3335
BstUI	CGCG	3 cut(s)	476, 696, 1255
BstV1I	GCAGC	9 cut(s)	374, 841, 1126, 1506, 2298, 2340, 2550, 2608, 2993
BstV2I	GAAGAC	3 cut(s)	160, 731, 3066
BstX2I	RGATCY	5 cut(s)	249, 1630, 1759, 2773, 2792
BstYI	RGATCY	5 cut(s)	249, 1630, 1759, 2773, 2792
Bsu15I	ATCGAT	2 cut(s)	2058, 2853
Bsu36I	CCTNAGG	1 cut(s)	81
BsuRI	GGCC	4 cut(s)	803, 1051, 1442, 1948
BsuTUI	ATCGAT	2 cut(s)	2058, 2853
BtgI	CCRYGG	1 cut(s)	345
BtrI	CACGTC	1 cut(s)	462
BtsI	GCAGTG	2 cut(s)	255, 1733
BtsIMutI	CAGTG	9 cut(s)	255, 629, 857, 931, 970, 1543, 1733, 2043, 2360
BtuMI	TCGCGA	1 cut(s)	476
BveI	ACCTGC	1 cut(s)	358
CciI	TCATGA	5 cut(s)	1059, 1483, 2653, 2760, 2967
CfoI	GCGC	3 cut(s)	367, 605, 3240
Cfr10I	RCCGGY	1 cut(s)	3262
Cfr13I	GGNCC	2 cut(s)	1049, 3531
ClaI	ATCGAT	2 cut(s)	2058, 2853
CseI	GACGC	1 cut(s)	154
Csp6I	GTAC	8 cut(s)	691, 788, 922, 2156, 2217, 2725, 2989, 3266
CspAI	ACCGGT	1 cut(s)	3262
CspCI	CAANNNNNGTGG	2 cut(s)	2161, 2196
CviQI	GTAC	8 cut(s)	691, 788, 922, 2156, 2217, 2725, 2989, 3266
DraI	TTTAAA	1 cut(s)	1218
DraIII	CACNNNGTG	2 cut(s)	1744, 3128
EaeI	YGGCCR	2 cut(s)	801, 1946
Eam1104I	CTCTTC	2 cut(s)	1684, 1712
EarI	CTCTTC	2 cut(s)	1684, 1712
Ecl136II	GAGCTC	1 cut(s)	2683
Eco130I	CCWWGG	1 cut(s)	53
Eco147I	AGGCCT	1 cut(s)	1442
Eco24I	GRGCYC	2 cut(s)	1707, 2685
Eco32I	GATATC	1 cut(s)	1314
Eco47I	GGWCC	1 cut(s)	3531
Eco53kI	GAGCTC	1 cut(s)	2683
Eco57I	CTGAAG	1 cut(s)	1988
Eco72I	CACGTG	2 cut(s)	814, 2519
Eco81I	CCTNAGG	1 cut(s)	81
Eco88I	CYCGRG	1 cut(s)	1697
EcoICRI	GAGCTC	1 cut(s)	2683
EcoNI	CCTNNNNNAGG	1 cut(s)	1233
EcoO109I	RGGNCCY	1 cut(s)	1049
EcoRI	GAATTC	2 cut(s)	1036, 2443
EcoRII	CCWGG	4 cut(s)	528, 1169, 1913, 2991
EcoRV	GATATC	1 cut(s)	1314
EcoT14I	CCWWGG	1 cut(s)	53
EcoT38I	GRGCYC	2 cut(s)	1707, 2685
ErhI	CCWWGG	1 cut(s)	53
Esp3I	CGTCTC	2 cut(s)	1956, 2952
FaqI	GGGAC	3 cut(s)	1808, 2335, 3544
FauI	CCCGC	2 cut(s)	185, 2991
FbaI	TGATCA	5 cut(s)	390, 655, 762, 1533, 1930
FblI	GTMKAC	2 cut(s)	1278, 3656
FriOI	GRGCYC	2 cut(s)	1707, 2685
FspAI	RTGCGCAY	1 cut(s)	3239
FspBI	CTAG	7 cut(s)	14, 200, 1143, 1446, 1469, 2976, 3675
FspI	TGCGCA	1 cut(s)	3239
GlaI	GCGC	3 cut(s)	366, 604, 3239
GsaI	CCCAGC	1 cut(s)	2537
HaeIII	GGCC	4 cut(s)	803, 1051, 1442, 1948
HapII	CCGG	4 cut(s)	752, 791, 2622, 3263
HgaI	GACGC	1 cut(s)	154
HhaI	GCGC	3 cut(s)	367, 605, 3240
Hin6I	GCGC	3 cut(s)	365, 603, 3238
HinP1I	GCGC	3 cut(s)	365, 603, 3238
HincII	GTYRAC	4 cut(s)	636, 1014, 2170, 3001
HindII	GTYRAC	4 cut(s)	636, 1014, 2170, 3001
HindIII	AAGCTT	1 cut(s)	2089
HpaI	GTTAAC	2 cut(s)	2170, 3001
HpaII	CCGG	4 cut(s)	752, 791, 2622, 3263
Hpy99I	CGWCG	2 cut(s)	176, 1045
HpyCH4IV	ACGT	7 cut(s)	310, 461, 813, 1010, 1298, 1866, 2518
HpySE526I	ACGT	7 cut(s)	310, 461, 813, 1010, 1298, 1866, 2518
HspAI	GCGC	3 cut(s)	365, 603, 3238
KpnI	GGTACC	2 cut(s)	694, 2220
Ksp22I	TGATCA	5 cut(s)	390, 655, 762, 1533, 1930
KspAI	GTTAAC	2 cut(s)	2170, 3001
LguI	GCTCTTC	1 cut(s)	1712
LmnI	GCTCC	9 cut(s)	63, 433, 552, 1027, 1100, 2266, 2593, 2680, 2688
Lsp1109I	GCAGC	9 cut(s)	374, 841, 1126, 1506, 2298, 2340, 2550, 2608, 2993
LweI	GCATC	8 cut(s)	610, 1328, 1411, 2634, 3113, 3337, 3465, 3604
MaeI	CTAG	7 cut(s)	14, 200, 1143, 1446, 1469, 2976, 3675
MaeII	ACGT	7 cut(s)	310, 461, 813, 1010, 1298, 1866, 2518
MfeI	CAATTG	1 cut(s)	3633
MflI	RGATCY	5 cut(s)	249, 1630, 1759, 2773, 2792
MhlI	GDGCHC	3 cut(s)	1707, 2579, 2685
MlsI	TGGCCA	1 cut(s)	803
MluNI	TGGCCA	1 cut(s)	803
MlyI	GAGTC	5 cut(s)	1193, 1489, 1790, 2084, 2909
MmeI	TCCRAC	4 cut(s)	894, 1644, 2022, 2688
Mox20I	TGGCCA	1 cut(s)	803
MroXI	GAANNNNTTC	1 cut(s)	2430
MscI	TGGCCA	1 cut(s)	803
MslI	CAYNNNNRTG	6 cut(s)	105, 194, 1334, 1419, 2395, 2868
Msp20I	TGGCCA	1 cut(s)	803
MspA1I	CMGCKG	1 cut(s)	3006
MspI	CCGG	4 cut(s)	752, 791, 2622, 3263
MspR9I	CCNGG	5 cut(s)	530, 1171, 1915, 2623, 2993
MunI	CAATTG	1 cut(s)	3633
Mva1269I	GAATGC	5 cut(s)	533, 1171, 1329, 2846, 3240
MvaI	CCWGG	4 cut(s)	530, 1171, 1915, 2993
MvnI	CGCG	3 cut(s)	476, 696, 1255
NciI	CCSGG	1 cut(s)	2623
NheI	GCTAGC	1 cut(s)	199
NlaIV	GGNNCC	4 cut(s)	692, 2218, 2531, 3532
NmeAIII	GCCGAG	1 cut(s)	1699
NmuCI	GTSAC	8 cut(s)	457, 712, 1145, 1412, 1862, 1907, 2519, 2828
NruI	TCGCGA	1 cut(s)	476
NsbI	TGCGCA	1 cut(s)	3239
NspI	RCATGY	1 cut(s)	1717
NspV	TTCGAA	2 cut(s)	2031, 2466
PaeR7I	CTCGAG	1 cut(s)	1697
PagI	TCATGA	5 cut(s)	1059, 1483, 2653, 2760, 2967
PceI	AGGCCT	1 cut(s)	1442
PciI	ACATGT	1 cut(s)	1713
PciSI	GCTCTTC	1 cut(s)	1712
PcsI	WCGNNNNNNNCGW	2 cut(s)	2515, 3545
PctI	GAATGC	5 cut(s)	533, 1171, 1329, 2846, 3240
PdmI	GAANNNNTTC	1 cut(s)	2430
PflMI	CCANNNNNTGG	2 cut(s)	54, 1744
PfoI	TCCNGGA	1 cut(s)	2621
PinAI	ACCGGT	1 cut(s)	3262
Ple19I	CGATCG	1 cut(s)	3552
PleI	GAGTC	5 cut(s)	1193, 1488, 1790, 2083, 2909
PmaCI	CACGTG	2 cut(s)	814, 2519
PmlI	CACGTG	2 cut(s)	814, 2519
PpsI	GAGTC	5 cut(s)	1193, 1488, 1790, 2083, 2909
Ppu21I	YACGTR	2 cut(s)	814, 2519
PscI	ACATGT	1 cut(s)	1713
Psp124BI	GAGCTC	1 cut(s)	2685
Psp1406I	AACGTT	1 cut(s)	1298
Psp6I	CCWGG	4 cut(s)	528, 1169, 1913, 2991
PspCI	CACGTG	2 cut(s)	814, 2519
PspFI	CCCAGC	1 cut(s)	2533
PspGI	CCWGG	4 cut(s)	528, 1169, 1913, 2991
PspN4I	GGNNCC	4 cut(s)	692, 2218, 2531, 3532
PspPI	GGNCC	2 cut(s)	1049, 3531
PspXI	VCTCGAGB	1 cut(s)	1697
PstI	CTGCAG	3 cut(s)	2257, 3273, 3339
PsuI	RGATCY	5 cut(s)	249, 1630, 1759, 2773, 2792
PvuI	CGATCG	1 cut(s)	3552
PvuII	CAGCTG	1 cut(s)	3006
RruI	TCGCGA	1 cut(s)	476
RsaI	GTAC	8 cut(s)	692, 789, 923, 2157, 2218, 2726, 2990, 3267
RsaNI	GTAC	8 cut(s)	691, 788, 922, 2156, 2217, 2725, 2989, 3266
RseI	CAYNNNNRTG	6 cut(s)	105, 194, 1334, 1419, 2395, 2868
SacI	GAGCTC	1 cut(s)	2685
SapI	GCTCTTC	1 cut(s)	1712
Sau96I	GGNCC	2 cut(s)	1049, 3531
SchI	GAGTC	5 cut(s)	1193, 1489, 1790, 2084, 2909
ScrFI	CCNGG	5 cut(s)	530, 1171, 1915, 2623, 2993
SduI	GDGCHC	3 cut(s)	1707, 2579, 2685
SfaNI	GCATC	8 cut(s)	610, 1328, 1411, 2634, 3113, 3337, 3465, 3604
SfcI	CTRYAG	6 cut(s)	160, 511, 912, 2253, 3269, 3335
Sfr274I	CTCGAG	1 cut(s)	1697
SfuI	TTCGAA	2 cut(s)	2031, 2466
SinI	GGWCC	1 cut(s)	3531
SlaI	CTCGAG	1 cut(s)	1697
SmiMI	CAYNNNNRTG	6 cut(s)	105, 194, 1334, 1419, 2395, 2868
SmlI	CTYRAG	3 cut(s)	987, 1697, 2846
SmoI	CTYRAG	3 cut(s)	987, 1697, 2846
SseBI	AGGCCT	1 cut(s)	1442
SspI	AATATT	1 cut(s)	1818
SspMI	CTAG	7 cut(s)	14, 200, 1143, 1446, 1469, 2976, 3675
SstI	GAGCTC	1 cut(s)	2685
StuI	AGGCCT	1 cut(s)	1442
StyD4I	CCNGG	5 cut(s)	528, 1169, 1913, 2621, 2991
StyI	CCWWGG	1 cut(s)	53
TaiI	ACGT	7 cut(s)	313, 464, 816, 1013, 1301, 1869, 2521
TaqII	GACCGA	1 cut(s)	3548
TatI	WGTACW	2 cut(s)	921, 2724
TauI	GCSGC	1 cut(s)	1508
TscAI	CASTG	9 cut(s)	262, 636, 864, 931, 970, 1543, 1733, 2050, 2360
TseFI	GTSAC	8 cut(s)	457, 712, 1145, 1412, 1862, 1907, 2519, 2828
TseI	GCWGC	9 cut(s)	362, 854, 1114, 1519, 2311, 2328, 2563, 2596, 3006
Tsp45I	GTSAC	8 cut(s)	457, 712, 1145, 1412, 1862, 1907, 2519, 2828
TspGWI	ACGGA	5 cut(s)	412, 1711, 2279, 3089, 3431
TspRI	CASTG	9 cut(s)	262, 636, 864, 931, 970, 1543, 1733, 2050, 2360
Van91I	CCANNNNNTGG	2 cut(s)	54, 1744
VpaK11BI	GGWCC	1 cut(s)	3531
XagI	CCTNNNNNAGG	1 cut(s)	1233
XapI	RAATTY	9 cut(s)	33, 340, 744, 1036, 1213, 1939, 2415, 2443, 3389
XceI	RCATGY	1 cut(s)	1717
XcmI	CCANNNNNNNNNTGG	2 cut(s)	134, 811
XhoI	CTCGAG	1 cut(s)	1697
XmiI	GTMKAC	2 cut(s)	1278, 3656
XmnI	GAANNNNTTC	1 cut(s)	2430
XspI	CTAG	7 cut(s)	14, 200, 1143, 1446, 1469, 2976, 3675

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.