Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008496

Physical Location & Seq

Genomic Coordinates Reverse (-)

19999610 .. 20005701

Gene Structure 6092 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1243500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3693 bp

ATGGCAACTCCTCTAGGAAAAGTGTTCGATAAAATTTCAGGTTTTGCCACACCTTGGAGAAAGAACAGTACTACAACTCCTCGGGGCAGAAGAATCTTTCATAGGGATGTTGAAAGAGACCAGTTCCAGTATGGAAATGCTCACTGCCTCTCCTCCTACTACAGCGTCTTTGTCGCCCGCCTTGCCATCATGGTTATGCTAGCCATTTTGATAGGGCTACTTACCATACTGACATGGCATTTCACAAGGATCTACACTGCAAAATCTCTAAGCAACTTAGCGTACGGGCTTCGCTACGAACTTCTGCAACGTCCAATTTTAAGGATGTGGAACATCTTGAATTCCACAGCTGAGATAACAGCAGCACAGGTTAAGCTCTCGGAGTATGTGATCAAACGGAACAGCAACCCTACTAGTCAAGCAGAACAAGTTGAGCTATATGCCTCGATGAGGGCTGTGACGTGGGCAATGTTTGTGAGTCGGAAGGCTCTGAATGCAATCACTATAAATTACAGAAATGGTTTTGTCCAGGCATTCCACAGAGACCACAGGAGCAACAACACATACTATATCTACTCTGATCTCGCAAACTATTCGATCAGCGCAAGTGGTGTGTACGATGCTAATATGCTGTCAACACATAAGGAGTGGAGTGATCAGTCCATACACGGCAACATTTCTGCAAAATGGTACCGCGAGCCACTTGACCCAGTCACAGGGGAGAAGACAGGGAAAGCAACCCAAATTCAGCCGGATGACCTCATCAACATTGCAGGGCTGTCACAAGTGCCGGATGGTGTGGCAACGTGGCACGTGGCAGTGAGCAAGTACTCGGATTCACCACTGCTTTCAGCTGCGCTGGCAGTTTCTGACCCTTCAAATAAAAGCATAGTGGCAGTTGTGGGTGTTACTACAGCTCTGTACAGTGTAGGGCAGCTGATGAAAGAGCTTGTAGAGTTTCACAGTGGACATATTTATTTAACTTCTCAGGAAGGTTACTTGCTTGCTACGTCAACAAATGCTCCTCTTTTGAGGAACTCGACGAGAGGCCCTAAGCTCATGATGGCTGTTGATTCTGAGGATCACATCATACACGCAGGAGCGGAGTGGTTACAGCGAGCCTATGGTGACAATTTTCCTCCTAGTCATGAGATTCATGTTGAGAATGCCAGGCTTGGAAACCAGCAGTATTACATCGACTCGTTTTTTCTGAAGTTAAAGAAACTTCCTCTGGTAGGAGTTATCATCATTCCGAGAAAATATATAATGGGAAAGGTAGATGAAAGAGCGTTGAAAACGTTGGTTATACTGATATCTGCATCCTTATGCATTCTGGTGATTGGATGTGTTTGTATTTTCATACTCACAAATGGAGTTTCAAAGGAAATGAAACTGAGGGCAGAGCTGATAAGTCACCTTGATGCAAGAAGAAGGGCCGAGGCATCTAGCAACTACAAAAGCCAATTTCTAGCCAATATGAGTCATGAACTGAGGACGCCAATGGCGGCAGTGATTGGGCTGCTGGACATTCTGATCAGTGATGATTGCCTTACAAATGAGCAATACTCCACAGTTACTCAGATCAGAAAATGCTCGACCGCTCTACTTCGGCTTCTCAACAACATATTAGATCTCAGCAAGGTTGAATCTGGAAAACTGGTGTTGGAAGAAGCCGAGTTTGATCTGGGAAGAGAACTCGAGGGGCTCTTCGACATGTTCTCTGTGCAGTGCAATAAGCACAATGTGGAGACTGTTTTAGATCTCTCTGATGATATGCCGAAATTAGTTCGAGGGGACTCTGCAAGAGTTGTTCAAATATTTGCTAATCTAATCAGTAACTCTATCAAGTTCACTACATCGGGTCACGTTATACTTAGAGGATCGTGTGAGAACTTGAACACTTCCGGTGACACCAGGAGAATCCTTCATGATCAGAAAAATTTGCCACCGTCTCATAAGGCAAAGCTGAAGCAAGGCAACCATGTTAAAAATGCTCCCAAGAAAGACAACAACAAAATGGTTCTTTGGTTCGAAGTTGATGACACTGGTTGCGGAATTGATCCGAGTAAATGGGAATCTGTTTTTGAAAGCTTTGAGCAAGCTGATCCCTCAACAACTCGAACACATGGTGGAACTGGACTTGGACTGTGCATTGTACGAACATTGGTTAACAAGATGGGTGGAGAAATCAAGGTTGTGAAGAAGGACGGGCAGGGTACTTTAATGCAATTTTACTTGGTTCTCAATACGCCTGCAGATGGCACGGAGCAACATTGTCAAGTGGATTTTGCAAAGCAAAATGTAGTGGTGCTGCTTGCACTACACGGCAGCATGGGGAGACTGATTATGTCCCAGTGGTTACGGAAAAATGGAGTGTTCACTTTGGCAACATCTGAGTGGAATGAACTGACACAAATTCTTCGAGAACTCTTTCATAGCAGGAATTCATCTCTTAAAAACAGCTTTGAAACACAGTATTCGCTGAGTGAATCTCTGAGAGGTGAAATACGAAACACATATGACATGAGGAACCCAGCTTTTCTCATGGTTGTCGATATAGGGCTGCTTGACTTGAGCACAGATATATGGAAGGAACAGCTTAACTTCCTTGACAAGTACTCGGGCAAAGCAAAGTTTGCATGGATACTGAATCATGATACATCCAACGCCGTGAAGATGGAGCTCCGAAGAAAAGGGTATATTTTGATGGTTAATAAACCGCTGTACAAGGCGAAAATGGTTCATATTTTGGAAGCTGTGATGAAAGAGAGAAATCTTGAAACGCAGAGTAGAAATGCAAATGGTTTGAGAACTACCACGAAAGAAGGTGACTTGCATGAATGCCTTGAGATTGATTCTATTCACTTCGATGTTGTCTTCAGCTCTGATGATTCTGATATATCCGAAAAGAACAACTCTAATTCTGAAAACACCTTTCATGACGAAGAAAAACAAAGAGACGGGATCATGAAACCCAGTTCATCGCAGTACCAAGAGGTTAACAGCTGCTTAGTTGAACTCACGCGCGTATGCTCGAAAGACAATAATTCAAGGAAAGAAGACATGGAAGACACAGAACATAAAGTAATGAGCAGTACTAGTCAAGCATCCCATGCAACAGAACCTCAATGCGAAAACTCAAAATTTCAAGAACAGAGTTCGACCACCAGCAGTTCTAAAGAGAATGGTAGTTCGTATACAAGTAGGGCGATGAATCAACAAAAATCTCTTGAAGGCGTGCGCATTCTTCTTGCAGAAGATACACCGGTACTGCAGAGAGTTGCAACCATAATGCTCGAAAAAATGGGGGCTACTGTGATTGCTGTGGCTGATGGACTGCAGGCAGTAGATGCTCTGAACTGTATGATCACTGCAGAAGATCGTGGAAGTGAATTTCACTCGAAAGACGGAGATACGAATTCAGATAATCAAAGTTGGGGTTTCCGTCCATTCGACTTGATTCTAATGGATTGTCAGATGCCGAAGATGGACGGTTATGAAGCAACGAAAGCAATCAGGAAATCGGAGGAGGGGACGGATTCACACATTCCGATCGTTGCCCTAACGGCTCATGCAATGTCATCAGATGAAGCAAAGTGCTTGGAGGTGGGGATGGATGCTTATTTAACAAAGCCAATTGACTACAAGTTGATGGTGTCTACCATTCTTTCACTCACTAGAACCACAGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005034 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009791 GO:0009927 GO:0009987 GO:0010035 GO:0010154 GO:0010375 GO:0010431 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0021700 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0032504 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071695 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HATPase_c HisKA Response_reg

Protein Analysis

1231

Amino Acids

137.79

Weight (kDa)

6.54

Isoelectric Point (pI)

37.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HisKA	PF00512	486 - 551	1.7e-17	His Kinase A (phospho-acceptor) domain
HATPase_c	PF02518	598 - 747	1.7e-19	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg	PF00072	1145 - 1221	2.4e-17	Response regulator receiver domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008496)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17820
fragaria_vesca	FvH4_1g11530 FvH4_1g11530 FvH4_1g11530 FvH4_1g11530 FvH4_1g11530 FvH4_1g11530
malus_domestica	MD02G1128300.v1.1 MD15G1243500.v1.1
prunus_persica	Prupe.7G170700_v2.0.a1 Prupe.7G170700_v2.0.a1
pyrus_communis	pycom02g10030
rosa_chinensis	RchiOBHm_Chr2g0099171
rosa_laevigata	RLG00000016852
rosa_multiflora	Rmu_sc0009113.1_g000004
rosa_roxburghii	Rroxscaffold_2G00143390
rosa_rugosa	Rorug02G0077300 Rorug02G0077400
rosa_samantha	Rh2AG126300 Rh2BG129500 Rh2CG130500 Rh2DG130900
rosa_wichuraiana	Rw2G009740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	3242
Acc65I	GGTACC	1 cut(s)	690
AccB1I	GGYRCC	1 cut(s)	690
AccB7I	CCANNNNNTGG	1 cut(s)	54
AccBSI	CCGCTC	2 cut(s)	1103, 1601
AccI	GTMKAC	2 cut(s)	3197, 3659
AccII	CGCG	3 cut(s)	696, 3025, 3027
AciI	CCGC	7 cut(s)	178, 694, 1103, 1505, 1599, 2052, 2720
AclI	AACGTT	1 cut(s)	1298
AclWI	GGATC	6 cut(s)	257, 1089, 1888, 2054, 2099, 2972
AcsI	RAATTY	9 cut(s)	33, 340, 744, 1939, 2415, 2443, 3143, 3392, 3418
AcuI	CTGAAG	3 cut(s)	1232, 1988, 2863
AcvI	CACGTG	1 cut(s)	814
AcyI	GRCGYC	1 cut(s)	1496
AdeI	CACNNNGTG	2 cut(s)	1744, 2129
AfiI	CCNNNNNNNGG	5 cut(s)	54, 450, 716, 1235, 2258
AflIII	ACRYGT	1 cut(s)	1713
AgeI	ACCGGT	1 cut(s)	3265
AhlI	ACTAGT	2 cut(s)	413, 3098
AjiI	CACGTC	1 cut(s)	462
AjnI	CCWGG	3 cut(s)	528, 1169, 1913
Alw21I	GWGCWC	2 cut(s)	2579, 2685
Alw26I	GTCTC	6 cut(s)	111, 537, 1742, 1956, 2332, 2952
AlwI	GGATC	6 cut(s)	257, 1089, 1888, 2054, 2099, 2972
AlwNI	CAGNNNCTG	1 cut(s)	869
Ama87I	CYCGRG	3 cut(s)	81, 1697, 2620
AoxI	GGCC	2 cut(s)	1048, 1434
ApeKI	GCWGC	8 cut(s)	362, 854, 934, 1519, 2311, 2328, 2563, 3006
ApoI	RAATTY	9 cut(s)	33, 340, 744, 1939, 2415, 2443, 3143, 3392, 3418
AsiGI	ACCGGT	1 cut(s)	3265
Asp700I	GAANNNNTTC	1 cut(s)	2430
Asp718I	GGTACC	1 cut(s)	690
AspLEI	GCGC	4 cut(s)	605, 859, 3027, 3243
AspS9I	GGNCC	2 cut(s)	1049, 1434
AsuHPI	GGTGA	7 cut(s)	831, 1139, 1348, 1406, 1919, 2513, 2840
AsuII	TTCGAA	1 cut(s)	2031
AsuNHI	GCTAGC	1 cut(s)	199
AvaI	CYCGRG	3 cut(s)	81, 1697, 2620
BaeI	ACNNNNGTAYC	2 cut(s)	3252, 3285
BanI	GGYRCC	1 cut(s)	690
BanII	GRGCYC	2 cut(s)	1707, 2685
BbrPI	CACGTG	1 cut(s)	814
BbsI	GAAGAC	4 cut(s)	731, 2869, 3066, 3075
Bbv12I	GWGCWC	2 cut(s)	2579, 2685
BbvI	GCAGC	8 cut(s)	374, 841, 946, 1506, 2298, 2340, 2550, 2993
BceAI	ACGGC	4 cut(s)	685, 2341, 2654, 3582
BcgI	CGANNNNNNTGC	2 cut(s)	576, 610
BciT130I	CCWGG	3 cut(s)	530, 1171, 1915
BciVI	GTATCC	1 cut(s)	2637
BclI	TGATCA	5 cut(s)	390, 655, 1533, 1930, 3366
BcoDI	GTCTC	6 cut(s)	111, 537, 1742, 1956, 2332, 2952
BcuI	ACTAGT	2 cut(s)	413, 3098
BfaI	CTAG	8 cut(s)	14, 200, 414, 1143, 1446, 1469, 3099, 3678
BfmI	CTRYAG	6 cut(s)	160, 912, 2253, 3272, 3338, 3372
BfuI	GTATCC	1 cut(s)	2637
BglI	GCCNNNNNGGC	1 cut(s)	3566
BglII	AGATCT	2 cut(s)	1630, 1759
BisI	GCNGC	9 cut(s)	363, 855, 935, 1506, 1520, 2312, 2329, 2564, 3007
BlsI	GCNGC	9 cut(s)	364, 856, 936, 1507, 1521, 2313, 2330, 2565, 3008
BmcAI	AGTACT	4 cut(s)	70, 830, 2618, 3097
Bme1390I	CCNGG	3 cut(s)	530, 1171, 1915
BmeT110I	CYCGRG	3 cut(s)	81, 1697, 2620
BmgBI	CACGTC	1 cut(s)	462
BmgT120I	GGNCC	2 cut(s)	1049, 1434
BmiI	GGNNCC	2 cut(s)	692, 2531
BmrFI	CCNGG	3 cut(s)	530, 1171, 1915
BmrI	ACTGGG	3 cut(s)	704, 2347, 2970
BmsI	GCATC	8 cut(s)	610, 1328, 1411, 1451, 3116, 3340, 3468, 3607
BmtI	GCTAGC	1 cut(s)	203
BmuI	ACTGGG	3 cut(s)	704, 2347, 2970
BpiI	GAAGAC	4 cut(s)	731, 2869, 3066, 3075
BplI	GAGNNNNNCTC	4 cut(s)	1550, 1582, 2476, 2508
Bpu10I	CCTNAGC	1 cut(s)	1053
Bpu14I	TTCGAA	1 cut(s)	2031
BpuEI	CTTGAG	2 cut(s)	2593, 2867
BsaAI	YACGTR	1 cut(s)	814
BsaHI	GRCGYC	1 cut(s)	1496
BsaI	GGTCTC	2 cut(s)	111, 537
BsaJI	CCNNGG	3 cut(s)	53, 80, 1437
BsaWI	WCCGGW	2 cut(s)	1904, 3265
BsaXI	ACNNNNNCTCC	6 cut(s)	61, 91, 134, 164, 1006, 1036
Bsc4I	CCNNNNNNNGG	5 cut(s)	54, 450, 716, 1235, 2258
Bse118I	RCCGGY	1 cut(s)	3265
Bse1I	ACTGG	8 cut(s)	121, 127, 710, 1662, 2050, 2140, 2353, 2976
Bse3DI	GCAATG	3 cut(s)	474, 768, 3582
BseBI	CCWGG	3 cut(s)	530, 1171, 1915
BseDI	CCNNGG	3 cut(s)	53, 80, 1437
BseLI	CCNNNNNNNGG	5 cut(s)	54, 450, 716, 1235, 2258
BseMI	GCAATG	3 cut(s)	474, 768, 3582
BseNI	ACTGG	8 cut(s)	121, 127, 710, 1662, 2050, 2140, 2353, 2976
BseRI	GAGGAG	4 cut(s)	69, 142, 1014, 3542
BseXI	GCAGC	8 cut(s)	374, 841, 946, 1506, 2298, 2340, 2550, 2993
BseYI	CCCAGC	1 cut(s)	2533
BsgI	GTGCAG	1 cut(s)	1745
Bsh1236I	CGCG	3 cut(s)	696, 3025, 3027
Bsh1285I	CGRYCG	2 cut(s)	1599, 3555
BshFI	GGCC	2 cut(s)	1050, 1436
BshNI	GGYRCC	1 cut(s)	690
BshTI	ACCGGT	1 cut(s)	3265
BsiEI	CGRYCG	2 cut(s)	1599, 3555
BsiHKAI	GWGCWC	2 cut(s)	2579, 2685
BsiHKCI	CYCGRG	3 cut(s)	81, 1697, 2620
BsiSI	CCGG	4 cut(s)	752, 791, 1905, 3266
BsiWI	CGTACG	1 cut(s)	282
BslFI	GGGAC	3 cut(s)	1808, 2335, 3547
BslI	CCNNNNNNNGG	5 cut(s)	54, 450, 716, 1235, 2258
BsmAI	GTCTC	6 cut(s)	111, 537, 1742, 1956, 2332, 2952
BsmBI	CGTCTC	2 cut(s)	1956, 2952
BsmFI	GGGAC	3 cut(s)	1808, 2335, 3547
BsmI	GAATGC	6 cut(s)	499, 533, 1171, 1329, 2846, 3243
BsnI	GGCC	2 cut(s)	1050, 1436
Bso31I	GGTCTC	2 cut(s)	111, 537
BsoBI	CYCGRG	3 cut(s)	81, 1697, 2620
Bsp119I	TTCGAA	1 cut(s)	2031
Bsp1286I	GDGCHC	3 cut(s)	1707, 2579, 2685
Bsp1407I	TGTACA	2 cut(s)	921, 2724
BspACI	CCGC	7 cut(s)	178, 694, 1103, 1505, 1599, 2052, 2720
BspANI	GGCC	2 cut(s)	1050, 1436
BspFNI	CGCG	3 cut(s)	696, 3025, 3027
BspHI	TCATGA	7 cut(s)	1059, 1147, 1483, 1927, 2653, 2938, 2967
BspLI	GGNNCC	2 cut(s)	692, 2531
BspMAI	CTGCAG	4 cut(s)	2257, 3276, 3342, 3376
BspOI	GCTAGC	1 cut(s)	203
BspPI	GGATC	6 cut(s)	257, 1089, 1888, 2054, 2099, 2972
BspQI	GCTCTTC	1 cut(s)	1712
BspT104I	TTCGAA	1 cut(s)	2031
BspT107I	GGYRCC	1 cut(s)	690
BspTNI	GGTCTC	2 cut(s)	111, 537
BsrBI	CCGCTC	2 cut(s)	1103, 1601
BsrDI	GCAATG	3 cut(s)	474, 768, 3582
BsrFI	RCCGGY	1 cut(s)	3265
BsrGI	TGTACA	2 cut(s)	921, 2724
BsrI	ACTGG	8 cut(s)	121, 127, 710, 1662, 2050, 2140, 2353, 2976
BssAI	RCCGGY	1 cut(s)	3265
BssECI	CCNNGG	3 cut(s)	53, 80, 1437
BssNAI	GTATAC	1 cut(s)	3198
BssNI	GRCGYC	1 cut(s)	1496
BssT1I	CCWWGG	1 cut(s)	53
Bst1107I	GTATAC	1 cut(s)	3198
Bst2UI	CCWGG	3 cut(s)	530, 1171, 1915
Bst6I	CTCTTC	2 cut(s)	1684, 1712
BstACI	GRCGYC	1 cut(s)	1496
BstAPI	GCANNNNNTGC	3 cut(s)	2636, 3113, 3350
BstAUI	TGTACA	2 cut(s)	921, 2724
BstBAI	YACGTR	1 cut(s)	814
BstBI	TTCGAA	1 cut(s)	2031
BstENI	CCTNNNNNAGG	2 cut(s)	448, 1233
BstFNI	CGCG	3 cut(s)	696, 3025, 3027
BstHHI	GCGC	4 cut(s)	605, 859, 3027, 3243
BstMAI	GTCTC	6 cut(s)	111, 537, 1742, 1956, 2332, 2952
BstMCI	CGRYCG	2 cut(s)	1599, 3555
BstMWI	GCNNNNNNNGC	8 cut(s)	182, 494, 860, 2636, 3113, 3350, 3509, 3566
BstNI	CCWGG	3 cut(s)	530, 1171, 1915
BstNSI	RCATGY	1 cut(s)	1717
BstSCI	CCNGG	3 cut(s)	528, 1169, 1913
BstSFI	CTRYAG	6 cut(s)	160, 912, 2253, 3272, 3338, 3372
BstUI	CGCG	3 cut(s)	696, 3025, 3027
BstV1I	GCAGC	8 cut(s)	374, 841, 946, 1506, 2298, 2340, 2550, 2993
BstV2I	GAAGAC	4 cut(s)	731, 2869, 3066, 3075
BstX2I	RGATCY	3 cut(s)	249, 1630, 1759
BstYI	RGATCY	3 cut(s)	249, 1630, 1759
BstZ17I	GTATAC	1 cut(s)	3198
BsuI	GTATCC	1 cut(s)	2637
BsuRI	GGCC	2 cut(s)	1050, 1436
BtgZI	GCGATG	2 cut(s)	2967, 3224
BtrI	CACGTC	1 cut(s)	462
BtsI	GCAGTG	7 cut(s)	142, 255, 825, 842, 1515, 1733, 3369
CaiI	CAGNNNCTG	1 cut(s)	869
CciI	TCATGA	7 cut(s)	1059, 1147, 1483, 1927, 2653, 2938, 2967
CfoI	GCGC	4 cut(s)	605, 859, 3027, 3243
Cfr10I	RCCGGY	1 cut(s)	3265
Cfr13I	GGNCC	2 cut(s)	1049, 1434
CseI	GACGC	2 cut(s)	154, 1504
CspAI	ACCGGT	1 cut(s)	3265
CspCI	CAANNNNNGTGG	2 cut(s)	2161, 2196
DraIII	CACNNNGTG	2 cut(s)	1744, 2129
Eam1104I	CTCTTC	2 cut(s)	1684, 1712
EarI	CTCTTC	2 cut(s)	1684, 1712
Ecl136II	GAGCTC	1 cut(s)	2683
Eco130I	CCWWGG	1 cut(s)	53
Eco24I	GRGCYC	2 cut(s)	1707, 2685
Eco31I	GGTCTC	2 cut(s)	111, 537
Eco32I	GATATC	1 cut(s)	1314
Eco53kI	GAGCTC	1 cut(s)	2683
Eco57I	CTGAAG	3 cut(s)	1232, 1988, 2863
Eco72I	CACGTG	1 cut(s)	814
Eco88I	CYCGRG	3 cut(s)	81, 1697, 2620
EcoICRI	GAGCTC	1 cut(s)	2683
EcoNI	CCTNNNNNAGG	2 cut(s)	448, 1233
EcoO109I	RGGNCCY	1 cut(s)	1049
EcoRI	GAATTC	3 cut(s)	340, 2443, 3418
EcoRII	CCWGG	3 cut(s)	528, 1169, 1913
EcoRV	GATATC	1 cut(s)	1314
EcoT14I	CCWWGG	1 cut(s)	53
EcoT22I	ATGCAT	1 cut(s)	1331
EcoT38I	GRGCYC	2 cut(s)	1707, 2685
ErhI	CCWWGG	1 cut(s)	53
Esp3I	CGTCTC	2 cut(s)	1956, 2952
FaqI	GGGAC	3 cut(s)	1808, 2335, 3547
FauI	CCCGC	1 cut(s)	185
FauNDI	CATATG	1 cut(s)	2518
FbaI	TGATCA	5 cut(s)	390, 655, 1533, 1930, 3366
FblI	GTMKAC	2 cut(s)	3197, 3659
Fnu4HI	GCNGC	9 cut(s)	363, 855, 935, 1506, 1520, 2312, 2329, 2564, 3007
FriOI	GRGCYC	2 cut(s)	1707, 2685
Fsp4HI	GCNGC	9 cut(s)	363, 855, 935, 1506, 1520, 2312, 2329, 2564, 3007
FspAI	RTGCGCAY	1 cut(s)	3242
FspBI	CTAG	8 cut(s)	14, 200, 414, 1143, 1446, 1469, 3099, 3678
FspI	TGCGCA	1 cut(s)	3242
GlaI	GCGC	4 cut(s)	604, 858, 3026, 3242
GluI	GCNGC	9 cut(s)	363, 855, 935, 1506, 1520, 2312, 2329, 2564, 3007
GsaI	CCCAGC	1 cut(s)	2537
HaeIII	GGCC	2 cut(s)	1050, 1436
HapII	CCGG	4 cut(s)	752, 791, 1905, 3266
HgaI	GACGC	2 cut(s)	154, 1504
HhaI	GCGC	4 cut(s)	605, 859, 3027, 3243
Hin1I	GRCGYC	1 cut(s)	1496
Hin6I	GCGC	4 cut(s)	603, 857, 3025, 3241
HinP1I	GCGC	4 cut(s)	603, 857, 3025, 3241
HincII	GTYRAC	4 cut(s)	636, 1014, 2170, 3001
HindII	GTYRAC	4 cut(s)	636, 1014, 2170, 3001
HindIII	AAGCTT	1 cut(s)	2089
HpaI	GTTAAC	2 cut(s)	2170, 3001
HpaII	CCGG	4 cut(s)	752, 791, 1905, 3266
HphI	GGTGA	7 cut(s)	831, 1139, 1348, 1406, 1919, 2513, 2840
Hpy99I	CGWCG	1 cut(s)	1045
HpyAV	CCTTC	9 cut(s)	478, 885, 986, 1425, 1934, 2197, 2584, 2819, 3227
HpyCH4IV	ACGT	7 cut(s)	310, 461, 806, 813, 1010, 1298, 1866
HpyF10VI	GCNNNNNNNGC	8 cut(s)	182, 494, 860, 2636, 3113, 3350, 3509, 3566
HpySE526I	ACGT	7 cut(s)	310, 461, 806, 813, 1010, 1298, 1866
Hsp92I	GRCGYC	1 cut(s)	1496
HspAI	GCGC	4 cut(s)	603, 857, 3025, 3241
KpnI	GGTACC	1 cut(s)	694
Ksp22I	TGATCA	5 cut(s)	390, 655, 1533, 1930, 3366
KspAI	GTTAAC	2 cut(s)	2170, 3001
LguI	GCTCTTC	1 cut(s)	1712
LmnI	GCTCC	7 cut(s)	552, 1027, 1100, 1999, 2266, 2680, 2688
Lsp1109I	GCAGC	8 cut(s)	374, 841, 946, 1506, 2298, 2340, 2550, 2993
LweI	GCATC	8 cut(s)	610, 1328, 1411, 1451, 3116, 3340, 3468, 3607
MaeI	CTAG	8 cut(s)	14, 200, 414, 1143, 1446, 1469, 3099, 3678
MaeII	ACGT	7 cut(s)	310, 461, 806, 813, 1010, 1298, 1866
MbiI	CCGCTC	2 cut(s)	1103, 1601
MfeI	CAATTG	1 cut(s)	3636
MflI	RGATCY	3 cut(s)	249, 1630, 1759
MhlI	GDGCHC	3 cut(s)	1707, 2579, 2685
MlyI	GAGTC	4 cut(s)	487, 1193, 1489, 1790
MmeI	TCCRAC	3 cut(s)	461, 1644, 2688
Mph1103I	ATGCAT	1 cut(s)	1331
MroXI	GAANNNNTTC	1 cut(s)	2430
MslI	CAYNNNNRTG	7 cut(s)	105, 194, 1324, 1334, 1419, 2395, 2868
MspA1I	CMGCKG	5 cut(s)	350, 854, 937, 2722, 3006
MspI	CCGG	4 cut(s)	752, 791, 1905, 3266
MspR9I	CCNGG	3 cut(s)	530, 1171, 1915
MunI	CAATTG	1 cut(s)	3636
Mva1269I	GAATGC	6 cut(s)	499, 533, 1171, 1329, 2846, 3243
MvaI	CCWGG	3 cut(s)	530, 1171, 1915
MvnI	CGCG	3 cut(s)	696, 3025, 3027
MwoI	GCNNNNNNNGC	8 cut(s)	182, 494, 860, 2636, 3113, 3350, 3509, 3566
NdeI	CATATG	1 cut(s)	2518
NheI	GCTAGC	1 cut(s)	199
NlaIV	GGNNCC	2 cut(s)	692, 2531
NmeAIII	GCCGAG	2 cut(s)	1462, 1699
NmuCI	GTSAC	8 cut(s)	457, 712, 780, 1127, 1412, 1862, 1907, 2828
NsbI	TGCGCA	1 cut(s)	3242
NsiI	ATGCAT	1 cut(s)	1331
NspI	RCATGY	1 cut(s)	1717
NspV	TTCGAA	1 cut(s)	2031
PaeR7I	CTCGAG	1 cut(s)	1697
PagI	TCATGA	7 cut(s)	1059, 1147, 1483, 1927, 2653, 2938, 2967
PciI	ACATGT	1 cut(s)	1713
PciSI	GCTCTTC	1 cut(s)	1712
PctI	GAATGC	6 cut(s)	499, 533, 1171, 1329, 2846, 3243
PdmI	GAANNNNTTC	1 cut(s)	2430
Pfl23II	CGTACG	1 cut(s)	282
PflFI	GACNNNGTC	1 cut(s)	710
PflMI	CCANNNNNTGG	1 cut(s)	54
PinAI	ACCGGT	1 cut(s)	3265
PkrI	GCNGC	9 cut(s)	364, 856, 936, 1507, 1521, 2313, 2330, 2565, 3008
Ple19I	CGATCG	1 cut(s)	3555
PleI	GAGTC	4 cut(s)	486, 1193, 1488, 1790
PmaCI	CACGTG	1 cut(s)	814
PmlI	CACGTG	1 cut(s)	814
PpsI	GAGTC	4 cut(s)	486, 1193, 1488, 1790
Ppu21I	YACGTR	1 cut(s)	814
PscI	ACATGT	1 cut(s)	1713
Psp124BI	GAGCTC	1 cut(s)	2685
Psp1406I	AACGTT	1 cut(s)	1298
Psp6I	CCWGG	3 cut(s)	528, 1169, 1913
PspCI	CACGTG	1 cut(s)	814
PspFI	CCCAGC	1 cut(s)	2533
PspGI	CCWGG	3 cut(s)	528, 1169, 1913
PspLI	CGTACG	1 cut(s)	282
PspN4I	GGNNCC	2 cut(s)	692, 2531
PspPI	GGNCC	2 cut(s)	1049, 1434
PspXI	VCTCGAGB	1 cut(s)	1697
PstI	CTGCAG	4 cut(s)	2257, 3276, 3342, 3376
PstNI	CAGNNNCTG	1 cut(s)	869
PsuI	RGATCY	3 cut(s)	249, 1630, 1759
PsyI	GACNNNGTC	1 cut(s)	710
PvuI	CGATCG	1 cut(s)	3555
PvuII	CAGCTG	4 cut(s)	350, 854, 937, 3006
RseI	CAYNNNNRTG	7 cut(s)	105, 194, 1324, 1334, 1419, 2395, 2868
SacI	GAGCTC	1 cut(s)	2685
SapI	GCTCTTC	1 cut(s)	1712
SatI	GCNGC	9 cut(s)	363, 855, 935, 1506, 1520, 2312, 2329, 2564, 3007
Sau96I	GGNCC	2 cut(s)	1049, 1434
ScaI	AGTACT	4 cut(s)	70, 830, 2618, 3097
SchI	GAGTC	4 cut(s)	487, 1193, 1489, 1790
ScrFI	CCNGG	3 cut(s)	530, 1171, 1915
SduI	GDGCHC	3 cut(s)	1707, 2579, 2685
SfaNI	GCATC	8 cut(s)	610, 1328, 1411, 1451, 3116, 3340, 3468, 3607
SfcI	CTRYAG	6 cut(s)	160, 912, 2253, 3272, 3338, 3372
Sfr274I	CTCGAG	1 cut(s)	1697
SfuI	TTCGAA	1 cut(s)	2031
SlaI	CTCGAG	1 cut(s)	1697
SmiMI	CAYNNNNRTG	7 cut(s)	105, 194, 1324, 1334, 1419, 2395, 2868
SmlI	CTYRAG	3 cut(s)	1697, 2572, 2846
SmoI	CTYRAG	3 cut(s)	1697, 2572, 2846
SpeI	ACTAGT	2 cut(s)	413, 3098
SsiI	CCGC	7 cut(s)	178, 694, 1103, 1505, 1599, 2052, 2720
SspI	AATATT	1 cut(s)	1818
SspMI	CTAG	8 cut(s)	14, 200, 414, 1143, 1446, 1469, 3099, 3678
SstI	GAGCTC	1 cut(s)	2685
StyD4I	CCNGG	3 cut(s)	528, 1169, 1913
StyI	CCWWGG	1 cut(s)	53
TaiI	ACGT	7 cut(s)	313, 464, 809, 816, 1013, 1301, 1869
TatI	WGTACW	6 cut(s)	68, 828, 921, 2616, 2724, 3095
TauI	GCSGC	1 cut(s)	1508
TseFI	GTSAC	8 cut(s)	457, 712, 780, 1127, 1412, 1862, 1907, 2828
TseI	GCWGC	8 cut(s)	362, 854, 934, 1519, 2311, 2328, 2563, 3006
Tsp45I	GTSAC	8 cut(s)	457, 712, 780, 1127, 1412, 1862, 1907, 2828
TspGWI	ACGGA	6 cut(s)	412, 2279, 2377, 3423, 3434, 3551
Tth111I	GACNNNGTC	1 cut(s)	710
Van91I	CCANNNNNTGG	1 cut(s)	54
XagI	CCTNNNNNAGG	2 cut(s)	448, 1233
XapI	RAATTY	9 cut(s)	33, 340, 744, 1939, 2415, 2443, 3143, 3392, 3418
XceI	RCATGY	1 cut(s)	1717
XcmI	CCANNNNNNNNNTGG	1 cut(s)	128
XhoI	CTCGAG	1 cut(s)	1697
XmiI	GTMKAC	2 cut(s)	3197, 3659
XmnI	GAANNNNTTC	1 cut(s)	2430
XspI	CTAG	8 cut(s)	14, 200, 414, 1143, 1446, 1469, 3099, 3678
ZrmI	AGTACT	4 cut(s)	70, 830, 2618, 3097
Zsp2I	ATGCAT	1 cut(s)	1331

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.